MD03G1230200.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000210
Physical Location & Seq
Reverse (-)
31536906 .. 31538995
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1230200.v1.1.491

Sequence Viewer

Length: 762 bp
ATGGCTAGGAAGCCAAAGAAACCATCATTTTTCAAGATCCTCCTTGGTGACTTCTCTCAGGGTCTGCGTATACCACCTACGTTTGTTAGGAAGAACTTCAATAGACGATCACTTAGCAAATGTGACCTTAAAGGCCCTACCGGAATACGAAGAACTGTGGAATTCGAAGACAGGGAGAATGGCATATTCTTCCGCAATGGTTGGCAGGGTTTTGTGAAGGACCATCATTTAGAAGCTGGGGATCTTTTGGTTTTTGACTATGATGGTGAAACAAAGTTTAATGTCAAAATCTATGATAGAAGTGCGTGCGAGAAAGATGTCGAAGCAGCCAAGATGAATATAGTAAATCAAGCTAGAGTAAAAGATGAAAATGTTGATGTTGCCACTGGAAACTATAACAAAGAGCGCAAAATCAGAACGACTAGCAGAAGTAGCAAGTATCTCATTTCTAGGAAAAGACGTTATTATGTAGAAGATACATCAACTGGATCTGTCTTGTTGAAATCGGAGAATCAATGTTTTCTAGCATTTTCAAACAAGAAACATTTACTACAATGCGTGAGGATCCCGAAGCAACTAGCGGTAGCTGAAGGCCTTATGTATAAGAATACTGTAACGATTCAAGATCCAGCTGGGCGTTCATGGCTTATTCAACTCAGAATCAGAAGGCCTAGTTCTACTGATCATTTGGACATTGCAACAGGTTGGACAGATTGCGTGAAAGCGAACCGAATTTCACCCGGGGACACCATGATTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

254

Amino Acids

29.12

Weight (kDa)

9.8

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 10 - 100 3.5e-17 B3 DNA binding domain
B3 PF02362 184 - 253 5.8e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66980
fragaria_vesca FvH4_2g27400 FvH4_2g27400 FvH4_2g27400 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_3g19710 FvH4_5g30470 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g23440 FvH4_7g23440 FvH4_7g23440
malus_domestica MD03G1230100.v1.1 MD03G1230200.v1.1 MD04G1034500.v1.1 MD04G1034600.v1.1 MD08G1039500.v1.1 MD11G1250800.v1.1 MD11G1251100.v1.1 MD11G1251200.v1.1
prunus_persica Prupe.1G388000_v2.0.a1 Prupe.1G388000_v2.0.a1 Prupe.1G388100_v2.0.a1 Prupe.4G178000_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178200_v2.0.a1 Prupe.4G178300_v2.0.a1 Prupe.4G178400_v2.0.a1 Prupe.4G178500_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206800_v2.0.a1 Prupe.4G206900_v2.0.a1 Prupe.4G207000_v2.0.a1 Prupe.7G064600_v2.0.a1 Prupe.7G064600_v2.0.a1
pyrus_communis pycom03g17770 pycom04g02860 pycom04g02870 pycom08g03160 pycom11g22190 pycom11g22200 pycom11g22210 pycom11g22220
rosa_chinensis RchiOBHm_Chr1g0318651 RchiOBHm_Chr1g0318881 RchiOBHm_Chr1g0319011 RchiOBHm_Chr1g0356381 RchiOBHm_Chr3g0482191 RchiOBHm_Chr5g0033031 RchiOBHm_Chr5g0033041 RchiOBHm_Chr6g0296251 RchiOBHm_Chr6g0299611 RchiOBHm_Chr7g0208501 RchiOBHm_Chr7g0224941
rosa_laevigata RLG00000001845 RLG00000011405 RLG00000011713 RLG00000023362 RLG00000030508 RLG00000030520 RLG00000033444
rosa_multiflora Rmu_sc0000307.1_g000013 Rmu_sc0000986.1_g000010 Rmu_sc0000986.1_g000011 Rmu_sc0002895.1_g000002 Rmu_sc0003503.1_g000005 Rmu_ssc0000357.1_g000023
rosa_roxburghii Rroxscaffold_1G00046900 Rroxscaffold_1G00046920 Rroxscaffold_1G00046930 Rroxscaffold_1G00046940 Rroxscaffold_1G00047010 Rroxscaffold_1G00047020 Rroxscaffold_1G00047030 Rroxscaffold_1G00059830 Rroxscaffold_3G00234250 Rroxscaffold_4G00299610 Rroxscaffold_4G00321350 Rroxscaffold_4G00328680 Rroxscaffold_4G00329000 Rroxscaffold_6G00399560 Rroxscaffold_6G00399970 Rroxscaffold_7G00168510 Rroxscaffold_7G00171700 Rroxscaffold_7G00178360
rosa_rugosa Rorug01G0022500 Rorug01G0022800 Rorug01G0026400 Rorug03G0195600 Rorug05G0135400 Rorug06G0260100 Rorug06G0260200.1 Rorug06G0291000 Rorug06G0291100 Rorug06G0291100 Rorug07G0227000
rosa_samantha Rh1AG035600 Rh1AG038500 Rh1AG045500 Rh1BG031400 Rh1BG034700 Rh1CG034500 Rh1CG037200 Rh1CG048500 Rh1DG032800 Rh1DG035800 Rh1DG052700 Rh3AG247000 Rh3CG277700 Rh3DG274200 Rh5AG227900 Rh5AG228000 Rh5CG256800 Rh5CG256900 Rh5CG257100 Rh5DG234000 Rh5DG234200 Rh6AG401800 Rh6BG380000 Rh6BG410200 Rh6CG385400 Rh6CG415800 Rh6DG372600 Rh6DG403000 Rh7AG376300 Rh7BG362400 Rh7DG372500
rosa_wichuraiana Rw0G008540 Rw1G002780 Rw1G002970 Rw3G022090 Rw5G020870 Rw6G032430 Rw6G035160 Rw7G031430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 70
AciI CCGC 2 cut(s) 193, 581
AclWI GGATC 6 cut(s) 31, 249, 496, 559, 572, 620
AcsI RAATTY 2 cut(s) 161, 732
AcuI CTGAAG 1 cut(s) 609
AgsI TTSAA 6 cut(s) 34, 100, 502, 534, 623, 653
AluBI AGCT 4 cut(s) 236, 353, 587, 632
AluI AGCT 4 cut(s) 236, 353, 587, 632
AlwI GGATC 6 cut(s) 31, 249, 496, 559, 572, 620
AlwNI CAGNNNCTG 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 740
AoxI GGCC 3 cut(s) 133, 592, 668
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 2 cut(s) 161, 732
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 2 cut(s) 134, 220
AsuC2I CCSGG 2 cut(s) 741, 742
AsuHPI GGTGA 3 cut(s) 59, 278, 729
AsuII TTCGAA 1 cut(s) 165
AvaI CYCGRG 1 cut(s) 740
AvaII GGWCC 1 cut(s) 220
BamHI GGATCC 1 cut(s) 564
BbsI GAAGAC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 338
BccI CCATC 3 cut(s) 31, 231, 257
BclI TGATCA 1 cut(s) 682
BcnI CCSGG 2 cut(s) 741, 742
BfaI CTAG 7 cut(s) 6, 354, 423, 450, 524, 578, 672
BisI GCNGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 328
Bme1390I CCNGG 2 cut(s) 741, 742
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 740
BmgT120I GGNCC 2 cut(s) 134, 220
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 2 cut(s) 741, 742
BpiI GAAGAC 1 cut(s) 174
Bpu14I TTCGAA 1 cut(s) 165
BpuMI CCSGG 2 cut(s) 741, 742
BsaJI CCNNGG 3 cut(s) 43, 740, 741
BsaWI WCCGGW 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 391, 490
Bse3DI GCAATG 2 cut(s) 202, 693
BseDI CCNNGG 3 cut(s) 43, 740, 741
BseMI GCAATG 2 cut(s) 202, 693
BseMII CTCAG 2 cut(s) 71, 670
BseNI ACTGG 2 cut(s) 391, 490
BseXI GCAGC 1 cut(s) 338
BseYI CCCAGC 2 cut(s) 236, 632
BshFI GGCC 3 cut(s) 135, 594, 670
BsiHKCI CYCGRG 1 cut(s) 740
BsiSI CCGG 2 cut(s) 141, 741
BslFI GGGAC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 3 cut(s) 135, 594, 670
BsoBI CYCGRG 1 cut(s) 740
Bsp119I TTCGAA 1 cut(s) 165
Bsp143I GATC 7 cut(s) 36, 107, 241, 488, 564, 625, 682
BspACI CCGC 2 cut(s) 193, 581
BspANI GGCC 3 cut(s) 135, 594, 670
BspCNI CTCAG 2 cut(s) 70, 669
BspLI GGNNCC 1 cut(s) 566
BspPI GGATC 6 cut(s) 31, 249, 496, 559, 572, 620
BspT104I TTCGAA 1 cut(s) 165
BsrDI GCAATG 2 cut(s) 202, 693
BsrI ACTGG 2 cut(s) 391, 490
BssECI CCNNGG 3 cut(s) 43, 740, 741
BssMI GATC 7 cut(s) 36, 107, 241, 488, 564, 625, 682
BssNAI GTATAC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 43
Bst1107I GTATAC 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 157, 613
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 307
BstDEI CTNAG 3 cut(s) 57, 113, 656
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 7 cut(s) 39, 110, 244, 491, 567, 628, 685
BstMBI GATC 7 cut(s) 36, 107, 241, 488, 564, 625, 682
BstMWI GCNNNNNNNGC 2 cut(s) 432, 643
BstSCI CCNGG 2 cut(s) 739, 740
BstV1I GCAGC 1 cut(s) 338
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 5 cut(s) 36, 241, 488, 564, 625
BstYI RGATCY 5 cut(s) 36, 241, 488, 564, 625
BstZ17I GTATAC 1 cut(s) 71
BsuRI GGCC 3 cut(s) 135, 594, 670
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 1 cut(s) 307
CaiI CAGNNNCTG 1 cut(s) 64
CfoI GCGC 1 cut(s) 408
Cfr13I GGNCC 2 cut(s) 134, 220
Cfr9I CCCGGG 1 cut(s) 740
CviAII CATG 2 cut(s) 642, 751
DdeI CTNAG 3 cut(s) 57, 113, 656
DpnI GATC 7 cut(s) 38, 109, 243, 490, 566, 627, 684
DpnII GATC 7 cut(s) 36, 107, 241, 488, 564, 625, 682
Eco130I CCWWGG 1 cut(s) 43
Eco147I AGGCCT 2 cut(s) 594, 670
Eco47I GGWCC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 609
Eco88I CYCGRG 1 cut(s) 740
EcoO109I RGGNCCY 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 2 cut(s) 645, 754
FaqI GGGAC 1 cut(s) 758
FatI CATG 2 cut(s) 641, 750
FbaI TGATCA 1 cut(s) 682
FblI GTMKAC 1 cut(s) 70
Fnu4HI GCNGC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 327
FspBI CTAG 7 cut(s) 6, 354, 423, 450, 524, 578, 672
GlaI GCGC 1 cut(s) 407
GluI GCNGC 1 cut(s) 327
GsaI CCCAGC 2 cut(s) 240, 636
HaeIII GGCC 3 cut(s) 135, 594, 670
HapII CCGG 2 cut(s) 141, 741
HhaI GCGC 1 cut(s) 408
Hin1II CATG 2 cut(s) 645, 754
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HinfI GANTC 3 cut(s) 511, 619, 660
HpaII CCGG 2 cut(s) 141, 741
HphI GGTGA 3 cut(s) 59, 278, 729
Hpy166II GTNNAC 1 cut(s) 71
Hpy188I TCNGA 4 cut(s) 416, 508, 659, 665
Hpy188III TCNNGA 3 cut(s) 34, 568, 623
Hpy8I GTNNAC 1 cut(s) 71
HpyAV CCTTC 3 cut(s) 211, 584, 660
HpyCH4III ACNGT 2 cut(s) 157, 613
HpyCH4IV ACGT 2 cut(s) 80, 460
HpyCH4V TGCA 1 cut(s) 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 643
HpyF3I CTNAG 3 cut(s) 57, 113, 656
HpySE526I ACGT 2 cut(s) 80, 460
Hsp92II CATG 2 cut(s) 645, 754
HspAI GCGC 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 682
Kzo9I GATC 7 cut(s) 36, 107, 241, 488, 564, 625, 682
Lsp1109I GCAGC 1 cut(s) 338
MaeI CTAG 7 cut(s) 6, 354, 423, 450, 524, 578, 672
MaeII ACGT 2 cut(s) 80, 460
MaeIII GTNAC 3 cut(s) 47, 122, 613
MalI GATC 7 cut(s) 38, 109, 243, 490, 566, 627, 684
MboI GATC 7 cut(s) 36, 107, 241, 488, 564, 625, 682
MboII GAAGA 5 cut(s) 103, 162, 179, 181, 485
MflI RGATCY 5 cut(s) 36, 241, 488, 564, 625
MluCI AATT 2 cut(s) 161, 732
MmeI TCCRAC 1 cut(s) 686
MnlI CCTC 2 cut(s) 50, 555
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 2 cut(s) 129, 279
MspA1I CMGCKG 1 cut(s) 632
MspI CCGG 2 cut(s) 141, 741
MspR9I CCNGG 2 cut(s) 741, 742
MwoI GCNNNNNNNGC 2 cut(s) 432, 643
NciI CCSGG 2 cut(s) 741, 742
NdeII GATC 7 cut(s) 36, 107, 241, 488, 564, 625, 682
NlaIII CATG 2 cut(s) 645, 754
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 2 cut(s) 47, 122
NspV TTCGAA 1 cut(s) 165
PceI AGGCCT 2 cut(s) 594, 670
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 3 cut(s) 511, 619, 660
PkrI GCNGC 1 cut(s) 328
PspFI CCCAGC 2 cut(s) 236, 632
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 2 cut(s) 134, 220
PstNI CAGNNNCTG 1 cut(s) 64
PsuI RGATCY 5 cut(s) 36, 241, 488, 564, 625
PvuII CAGCTG 1 cut(s) 632
SaqAI TTAA 2 cut(s) 129, 279
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 7 cut(s) 36, 107, 241, 488, 564, 625, 682
Sau96I GGNCC 2 cut(s) 134, 220
ScrFI CCNGG 2 cut(s) 741, 742
SetI ASST 9 cut(s) 79, 83, 129, 238, 355, 463, 589, 634, 706
SfuI TTCGAA 1 cut(s) 165
SinI GGWCC 1 cut(s) 220
SmaI CCCGGG 1 cut(s) 742
Sse9I AATT 2 cut(s) 161, 732
SseBI AGGCCT 2 cut(s) 594, 670
SsiI CCGC 2 cut(s) 193, 581
SspMI CTAG 7 cut(s) 6, 354, 423, 450, 524, 578, 672
StuI AGGCCT 2 cut(s) 594, 670
StyD4I CCNGG 2 cut(s) 739, 740
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 2 cut(s) 157, 613
TaiI ACGT 2 cut(s) 83, 463
TaqI TCGA 2 cut(s) 165, 321
TasI AATT 2 cut(s) 161, 732
TfiI GAWTC 3 cut(s) 511, 619, 660
Tru1I TTAA 2 cut(s) 129, 279
Tru9I TTAA 2 cut(s) 129, 279
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 2 cut(s) 47, 122
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 2 cut(s) 47, 122
TspDTI ATGAA 3 cut(s) 350, 381, 630
TspMI CCCGGG 1 cut(s) 740
TspRI CASTG 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 2 cut(s) 161, 732
XmaI CCCGGG 1 cut(s) 740
XmiI GTMKAC 1 cut(s) 70
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 7 cut(s) 6, 354, 423, 450, 524, 578, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.