Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000141

Physical Location & Seq

Genomic Coordinates Forward (+)

30347873 .. 30349041

Gene Structure 1169 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1238600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 939 bp

ATGGAGTTCCTTAACATGAAGCTGTGGAAATATATGTGCCTTAGCTTGATCCTCACCTTGGGGGCTTGGTCTTCTGAAGCCACTTCTCGTACTCTGCAAGATGCATCTATGTATGGGAGATACGAGCAATGGTTGATTCGTTATGGTTGCGTATATAATGACATTAATGAGAAGGAAACTCCTTTCAAGATATTCAAGGAGAGTTTTGCAGACCTTACAAATGAAGAGTTCAAAGCCTCAAGAAATGGATTCAACGGACATGAGTGCTCCACGAAGACAACTTGTTTCAAGTATGAAAATGTGACTGCATCATTACCAGCAACAATGGACTGGAGAGAGAAAGGAGCAGTAACCCCCATCAAGGACCAAGGCCAATGCGGTAGCTGTTGGGCCTTTTCAGCAGTGGCCGCCACTGAAGGAATAACACAGCTTACAACTGGGAAATTGATCTCTTTGTCTGAGCAAGAGCTTGTTGATTGTGATACCGCTGGTGAAGACCAGGGATGTGAGGGTGGCTTGATGGACGACGCCTTCCAGTTCATCCAAAAAAATCACGGGATTAGCACAGAAGCTAATTACCCCTACAACGGCGTTGAAGGTACATGTAACACCAAGAAGGAAGCAAGCCATGCAGCCAAGATAACTGGCTTGAAGATGTCCCTGCAAACATTCTATTCAAGTGGTGTCTTTACCGGAACTTGTGGAACGAGCCTTGACCATGGTGTTACCGCTGTTGGTTATGGCCTGAGTGAGGATGGGACTAAGTATTGGTTAGTGAAGAACTCATGGGGTGCAGAATGGGATGAAGAGGGGTACATTAGGATGCAAAGAGATGTTGCAGCAGCCGAAGGACTTTGTGGAATTGCTGTCTGCATCTTACCCCACAGTCTGCCGCATACAGACCTACGCCACACTTTCCTACATGGCAATCACTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

313

Amino Acids

34.56

Weight (kDa)

5.15

Isoelectric Point (pI)

23.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_C1	PF00112	105 - 218	1.8e-43	Papain family cysteine protease
Peptidase_C1	PF00112	223 - 289	6.4e-21	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000141)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22160
fragaria_vesca	FvH4_1g23580 FvH4_1g25982 FvH4_2g17070 FvH4_3g40500 FvH4_6g27180 FvH4_6g27320 FvH4_6g33300 FvH4_6g33310 FvH4_7g09480 FvH4_7g13000 FvH4_7g13320 FvH4_7g13600
malus_domestica	MD00G1208000.v1.1 MD09G1238200.v1.1 MD09G1238600.v1.1 MD09G1238800.v1.1 MD09G1239100.v1.1 MD16G1226900.v1.1 MD16G1242700.v1.1 MD16G1244200.v1.1 MD16G1245500.v1.1 MD16G1245800.v1.1 MD17G1231600.v1.1 MD17G1231700.v1.1 MD17G1231800.v1.1 MD17G1232200.v1.1 MD17G1232300.v1.1 MD17G1232400.v1.1
prunus_persica	Prupe.1G072300_v2.0.a1 Prupe.1G072400_v2.0.a1 Prupe.3G106300_v2.0.a1 Prupe.3G106400_v2.0.a1 Prupe.3G119400_v2.0.a1 Prupe.3G120600_v2.0.a1 Prupe.3G121900_v2.0.a1 Prupe.3G122000_v2.0.a1 Prupe.3G122100_v2.0.a1 Prupe.3G122200_v2.0.a1 Prupe.3G122700_v2.0.a1 Prupe.5G055900_v2.0.a1 Prupe.5G056500_v2.0.a1
pyrus_communis	pycom09g15770 pycom09g15780 pycom09g15790 pycom16g20640 pycom17g18390
rosa_chinensis	RchiOBHm_Chr1g0352141 RchiOBHm_Chr1g0352581 RchiOBHm_Chr1g0352601 RchiOBHm_Chr1g0352621 RchiOBHm_Chr1g0352631 RchiOBHm_Chr2g0119131 RchiOBHm_Chr2g0128691 RchiOBHm_Chr2g0128701 RchiOBHm_Chr2g0129151 RchiOBHm_Chr2g0129221 RchiOBHm_Chr2g0129401 RchiOBHm_Chr2g0129421 RchiOBHm_Chr2g0129431 RchiOBHm_Chr2g0129471 RchiOBHm_Chr2g0129481 RchiOBHm_Chr2g0129491 RchiOBHm_Chr2g0143051 RchiOBHm_Chr2g0143121 RchiOBHm_Chr2g0143171 RchiOBHm_Chr3g0457341 RchiOBHm_Chr5g0049011 RchiOBHm_Chr6g0281281 RchiOBHm_Chr6g0281311
rosa_laevigata	RLG00000012975 RLG00000012976 RLG00000012979 RLG00000018447 RLG00000019018 RLG00000019061 RLG00000019066 RLG00000019078 RLG00000019079 RLG00000019080 RLG00000019083 RLG00000019085 RLG00000020009 RLG00000020010 RLG00000025234 RLG00000028326 RLG00000028327 RLG00000028328 RLG00000028329 RLG00000028357 RLG00000031784
rosa_multiflora	Rmu_co8169188.1_g000001 Rmu_co8261007.1_g000001 Rmu_co8314361.1_g000001 Rmu_sc0000109.1_g000014 Rmu_sc0000638.1_g000020 Rmu_sc0000847.1_g000020 Rmu_sc0000847.1_g000028 Rmu_sc0000847.1_g000037 Rmu_sc0000847.1_g000046 Rmu_sc0000911.1_g000034 Rmu_sc0000999.1_g000021 Rmu_sc0001171.1_g000013 Rmu_sc0001171.1_g000018 Rmu_sc0001171.1_g000019 Rmu_sc0001653.1_g000004 Rmu_sc0002277.1_g000005 Rmu_sc0003479.1_g000001 Rmu_sc0005118.1_g000006 Rmu_sc0005118.1_g000008 Rmu_sc0005227.1_g000012 Rmu_sc0005442.1_g000010 Rmu_sc0005514.1_g000005 Rmu_sc0005996.1_g000014 Rmu_sc0008800.1_g000002 Rmu_sc0008800.1_g000010 Rmu_sc0008800.1_g000014
rosa_roxburghii	Rroxscaffold_1G00065560 Rroxscaffold_2G00102080 Rroxscaffold_2G00102090 Rroxscaffold_2G00102110 Rroxscaffold_2G00114560 Rroxscaffold_2G00114570 Rroxscaffold_2G00114610 Rroxscaffold_2G00114620 Rroxscaffold_2G00114640 Rroxscaffold_2G00114740 Rroxscaffold_2G00114790 Rroxscaffold_2G00115860 Rroxscaffold_2G00124490 Rroxscaffold_4G00302260 Rroxscaffold_4G00302530 Rroxscaffold_4G00302550 Rroxscaffold_4G00302560 Rroxscaffold_4G00302620 Rroxscaffold_6G00421980 Rroxscaffold_7G00187140 Rroxscaffold_7G00187170
rosa_rugosa	Rorug01G0225000 Rorug01G0230200 Rorug02G0220500 Rorug02G0280000 Rorug02G0285200 Rorug02G0285400 Rorug02G0286300 Rorug02G0286300 Rorug02G0286400 Rorug02G0286900 Rorug02G0377800 Rorug02G0377900 Rorug02G0378000 Rorug02G0378000 Rorug02G0378800 Rorug03G0019800 Rorug04G0452700 Rorug06G0140300 Rorug06G0140500 Rorug06G0140600
rosa_samantha	Rh1CG223600 Rh1CG227200 Rh1CG227500 Rh1CG227600 Rh2BG341200 Rh2BG345300 Rh2BG440200 Rh2DG284500 Rh2DG357900 Rh2DG362100 Rh2DG362600 Rh2DG364500 Rh2DG364600 Rh2DG364700 Rh2DG364800 Rh2DG364900 Rh2DG365000 Rh2DG365400 Rh2DG450400 Rh3AG080000 Rh3BG081900 Rh5AG322100 Rh5BG079800 Rh6AG252100 Rh6BG255600
rosa_wichuraiana	Rw1G020770 Rw1G021290 Rw2G022120 Rw2G026940 Rw2G027360 Rw2G027370 Rw2G027950 Rw2G027960 Rw2G027970 Rw2G035220 Rw3G006890 Rw6G021900 Rw6G021930 Rw6G021950 Rw6G021970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	5 cut(s)	378, 408, 486, 729, 893
AclWI	GGATC	1 cut(s)	43
AcoI	YGGCCR	1 cut(s)	405
AcuI	CTGAAG	2 cut(s)	96, 435
AcyI	GRCGYC	1 cut(s)	528
AfaI	GTAC	3 cut(s)	91, 601, 815
AfiI	CCNNNNNNNGG	4 cut(s)	58, 361, 587, 751
AflIII	ACRYGT	1 cut(s)	602
AgsI	TTSAA	8 cut(s)	187, 196, 232, 253, 289, 596, 652, 678
AjnI	CCWGG	1 cut(s)	498
AluBI	AGCT	6 cut(s)	22, 45, 384, 430, 469, 572
AluI	AGCT	6 cut(s)	22, 45, 384, 430, 469, 572
Alw21I	GWGCWC	1 cut(s)	269
AlwI	GGATC	1 cut(s)	43
AoxI	GGCC	4 cut(s)	370, 390, 405, 742
ApeKI	GCWGC	3 cut(s)	632, 839, 842
AseI	ATTAAT	1 cut(s)	165
AspS9I	GGNCC	2 cut(s)	364, 390
AsuHPI	GGTGA	2 cut(s)	46, 503
AvaII	GGWCC	1 cut(s)	364
BbsI	GAAGAC	3 cut(s)	63, 281, 501
Bbv12I	GWGCWC	1 cut(s)	269
BbvI	GCAGC	3 cut(s)	644, 851, 854
BccI	CCATC	3 cut(s)	365, 514, 749
BceAI	ACGGC	1 cut(s)	604
BciT130I	CCWGG	1 cut(s)	500
BisI	GCNGC	5 cut(s)	408, 633, 840, 843, 893
BlsI	GCNGC	5 cut(s)	409, 634, 841, 844, 894
Bme1390I	CCNGG	1 cut(s)	500
Bme18I	GGWCC	1 cut(s)	364
BmgT120I	GGNCC	2 cut(s)	364, 390
BmrFI	CCNGG	1 cut(s)	500
BmrI	ACTGGG	1 cut(s)	447
BmsI	GCATC	5 cut(s)	91, 113, 317, 813, 882
BmuI	ACTGGG	1 cut(s)	447
BpiI	GAAGAC	3 cut(s)	63, 281, 501
BpmI	CTGGAG	1 cut(s)	352
Bpu10I	CCTNAGC	1 cut(s)	41
BpuEI	CTTGAG	1 cut(s)	223
BsaHI	GRCGYC	1 cut(s)	528
BsaJI	CCNNGG	4 cut(s)	57, 367, 499, 718
BsaWI	WCCGGW	1 cut(s)	692
Bsc4I	CCNNNNNNNGG	4 cut(s)	58, 361, 587, 751
Bse1I	ACTGG	4 cut(s)	335, 442, 535, 649
Bse3DI	GCAATG	1 cut(s)	134
BseBI	CCWGG	1 cut(s)	500
BseDI	CCNNGG	4 cut(s)	57, 367, 499, 718
BseGI	GGATG	5 cut(s)	509, 540, 760, 808, 828
BseLI	CCNNNNNNNGG	4 cut(s)	58, 361, 587, 751
BseMI	GCAATG	1 cut(s)	134
BseMII	CTCAG	2 cut(s)	450, 737
BseNI	ACTGG	4 cut(s)	335, 442, 535, 649
BseXI	GCAGC	3 cut(s)	644, 851, 854
BsgI	GTGCAG	1 cut(s)	813
BshFI	GGCC	4 cut(s)	372, 392, 407, 744
BsiHKAI	GWGCWC	1 cut(s)	269
BsiSI	CCGG	1 cut(s)	693
BslFI	GGGAC	2 cut(s)	643, 772
BslI	CCNNNNNNNGG	4 cut(s)	58, 361, 587, 751
BsmFI	GGGAC	2 cut(s)	643, 772
BsnI	GGCC	4 cut(s)	372, 392, 407, 744
Bsp1286I	GDGCHC	1 cut(s)	269
Bsp143I	GATC	2 cut(s)	48, 447
Bsp19I	CCATGG	1 cut(s)	718
BspACI	CCGC	5 cut(s)	378, 408, 486, 729, 893
BspANI	GGCC	4 cut(s)	372, 392, 407, 744
BspCNI	CTCAG	2 cut(s)	451, 738
BspPI	GGATC	1 cut(s)	43
BsrDI	GCAATG	1 cut(s)	134
BsrI	ACTGG	4 cut(s)	335, 442, 535, 649
BssECI	CCNNGG	4 cut(s)	57, 367, 499, 718
BssMI	GATC	2 cut(s)	48, 447
BssNI	GRCGYC	1 cut(s)	528
BssT1I	CCWWGG	3 cut(s)	57, 367, 718
Bst2UI	CCWGG	1 cut(s)	500
Bst4CI	ACNGT	1 cut(s)	887
Bst6I	CTCTTC	2 cut(s)	219, 801
BstACI	GRCGYC	1 cut(s)	528
BstAPI	GCANNNNNTGC	1 cut(s)	629
BstC8I	GCNNGC	1 cut(s)	625
BstDEI	CTNAG	4 cut(s)	41, 459, 746, 762
BstDSI	CCRYGG	1 cut(s)	718
BstENI	CCTNNNNNAGG	1 cut(s)	749
BstF5I	GGATG	5 cut(s)	509, 540, 760, 808, 828
BstKTI	GATC	2 cut(s)	51, 450
BstMBI	GATC	2 cut(s)	48, 447
BstMWI	GCNNNNNNNGC	3 cut(s)	398, 407, 629
BstNI	CCWGG	1 cut(s)	500
BstNSI	RCATGY	1 cut(s)	606
BstSCI	CCNGG	1 cut(s)	498
BstV1I	GCAGC	3 cut(s)	644, 851, 854
BstV2I	GAAGAC	3 cut(s)	63, 281, 501
BsuRI	GGCC	4 cut(s)	372, 392, 407, 744
BtgI	CCRYGG	1 cut(s)	718
BtsCI	GGATG	5 cut(s)	509, 540, 760, 808, 828
BtsI	GCAGTG	1 cut(s)	408
BtsIMutI	CAGTG	2 cut(s)	408, 411
Cac8I	GCNNGC	1 cut(s)	625
Cfr13I	GGNCC	2 cut(s)	364, 390
CseI	GACGC	1 cut(s)	536
Csp6I	GTAC	3 cut(s)	90, 600, 814
CviAII	CATG	7 cut(s)	16, 260, 603, 629, 719, 786, 923
CviQI	GTAC	3 cut(s)	90, 600, 814
DdeI	CTNAG	4 cut(s)	41, 459, 746, 762
DpnI	GATC	2 cut(s)	50, 449
DpnII	GATC	2 cut(s)	48, 447
EaeI	YGGCCR	1 cut(s)	405
Eam1104I	CTCTTC	2 cut(s)	219, 801
EarI	CTCTTC	2 cut(s)	219, 801
Eco130I	CCWWGG	3 cut(s)	57, 367, 718
Eco47I	GGWCC	1 cut(s)	364
Eco57I	CTGAAG	2 cut(s)	96, 435
EcoNI	CCTNNNNNAGG	1 cut(s)	749
EcoRII	CCWGG	1 cut(s)	498
EcoT14I	CCWWGG	3 cut(s)	57, 367, 718
EcoT22I	ATGCAT	1 cut(s)	106
ErhI	CCWWGG	3 cut(s)	57, 367, 718
FaeI	CATG	7 cut(s)	19, 263, 606, 632, 722, 789, 926
FaqI	GGGAC	2 cut(s)	643, 772
FatI	CATG	7 cut(s)	15, 259, 602, 628, 718, 785, 922
Fnu4HI	GCNGC	5 cut(s)	408, 633, 840, 843, 893
FokI	GGATG	5 cut(s)	516, 527, 767, 815, 835
Fsp4HI	GCNGC	5 cut(s)	408, 633, 840, 843, 893
GluI	GCNGC	5 cut(s)	408, 633, 840, 843, 893
GsuI	CTGGAG	1 cut(s)	352
HaeIII	GGCC	4 cut(s)	372, 392, 407, 744
HapII	CCGG	1 cut(s)	693
HgaI	GACGC	1 cut(s)	536
Hin1I	GRCGYC	1 cut(s)	528
Hin1II	CATG	7 cut(s)	19, 263, 606, 632, 722, 789, 926
HinfI	GANTC	2 cut(s)	136, 249
HpaII	CCGG	1 cut(s)	693
HphI	GGTGA	2 cut(s)	46, 503
Hpy188I	TCNGA	2 cut(s)	76, 460
Hpy188III	TCNNGA	2 cut(s)	187, 240
Hpy99I	CGWCG	1 cut(s)	530
HpyAV	CCTTC	6 cut(s)	166, 410, 541, 590, 610, 842
HpyCH4III	ACNGT	1 cut(s)	887
HpyF10VI	GCNNNNNNNGC	3 cut(s)	398, 407, 629
HpyF3I	CTNAG	4 cut(s)	41, 459, 746, 762
Hsp92I	GRCGYC	1 cut(s)	528
Hsp92II	CATG	7 cut(s)	19, 263, 606, 632, 722, 789, 926
Kzo9I	GATC	2 cut(s)	48, 447
LmnI	GCTCC	2 cut(s)	272, 344
Lsp1109I	GCAGC	3 cut(s)	644, 851, 854
LweI	GCATC	5 cut(s)	91, 113, 317, 813, 882
MaeIII	GTNAC	4 cut(s)	301, 349, 605, 724
MalI	GATC	2 cut(s)	50, 449
MboI	GATC	2 cut(s)	48, 447
MboII	GAAGA	7 cut(s)	63, 236, 286, 506, 664, 790, 818
MhlI	GDGCHC	1 cut(s)	269
MluCI	AATT	3 cut(s)	443, 574, 861
MnlI	CCTC	5 cut(s)	62, 247, 502, 745, 802
Mph1103I	ATGCAT	1 cut(s)	106
MseI	TTAA	3 cut(s)	12, 165, 937
MslI	CAYNNNNRTG	1 cut(s)	821
MspA1I	CMGCKG	2 cut(s)	488, 731
MspI	CCGG	1 cut(s)	693
MspR9I	CCNGG	1 cut(s)	500
MvaI	CCWGG	1 cut(s)	500
MwoI	GCNNNNNNNGC	3 cut(s)	398, 407, 629
NcoI	CCATGG	1 cut(s)	718
NdeII	GATC	2 cut(s)	48, 447
NlaIII	CATG	7 cut(s)	19, 263, 606, 632, 722, 789, 926
NmuCI	GTSAC	1 cut(s)	301
NsiI	ATGCAT	1 cut(s)	106
NspI	RCATGY	1 cut(s)	606
PciI	ACATGT	1 cut(s)	602
PfeI	GAWTC	2 cut(s)	136, 249
PkrI	GCNGC	5 cut(s)	409, 634, 841, 844, 894
PscI	ACATGT	1 cut(s)	602
PshBI	ATTAAT	1 cut(s)	165
Psp6I	CCWGG	1 cut(s)	498
PspGI	CCWGG	1 cut(s)	498
PspPI	GGNCC	2 cut(s)	364, 390
RsaI	GTAC	3 cut(s)	91, 601, 815
RsaNI	GTAC	3 cut(s)	90, 600, 814
RseI	CAYNNNNRTG	1 cut(s)	821
SaqAI	TTAA	3 cut(s)	12, 165, 937
SatI	GCNGC	5 cut(s)	408, 633, 840, 843, 893
Sau3AI	GATC	2 cut(s)	48, 447
Sau96I	GGNCC	2 cut(s)	364, 390
ScrFI	CCNGG	1 cut(s)	500
SduI	GDGCHC	1 cut(s)	269
SfaNI	GCATC	5 cut(s)	91, 113, 317, 813, 882
SinI	GGWCC	1 cut(s)	364
SmiMI	CAYNNNNRTG	1 cut(s)	821
SmlI	CTYRAG	1 cut(s)	238
SmoI	CTYRAG	1 cut(s)	238
Sse9I	AATT	3 cut(s)	443, 574, 861
SsiI	CCGC	5 cut(s)	378, 408, 486, 729, 893
StyD4I	CCNGG	1 cut(s)	498
StyI	CCWWGG	3 cut(s)	57, 367, 718
TaaI	ACNGT	1 cut(s)	887
TasI	AATT	3 cut(s)	443, 574, 861
TauI	GCSGC	2 cut(s)	410, 895
TfiI	GAWTC	2 cut(s)	136, 249
Tru1I	TTAA	3 cut(s)	12, 165, 937
Tru9I	TTAA	3 cut(s)	12, 165, 937
TscAI	CASTG	2 cut(s)	408, 418
TseFI	GTSAC	1 cut(s)	301
TseI	GCWGC	3 cut(s)	632, 839, 842
Tsp45I	GTSAC	1 cut(s)	301
TspDTI	ATGAA	5 cut(s)	32, 237, 309, 529, 819
TspGWI	ACGGA	1 cut(s)	270
TspRI	CASTG	2 cut(s)	408, 418
VpaK11BI	GGWCC	1 cut(s)	364
VspI	ATTAAT	1 cut(s)	165
XagI	CCTNNNNNAGG	1 cut(s)	749
XceI	RCATGY	1 cut(s)	606
Zsp2I	ATGCAT	1 cut(s)	106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.