Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 530 |
| AccI | GTMKAC | 1 cut(s) | 144 |
| AciI | CCGC | 2 cut(s) | 79, 855 |
| AclWI | GGATC | 2 cut(s) | 37, 790 |
| AcoI | YGGCCR | 1 cut(s) | 694 |
| AcuI | CTGAAG | 1 cut(s) | 87 |
| AcyI | GRCGYC | 1 cut(s) | 870 |
| AfaI | GTAC | 2 cut(s) | 649, 941 |
| AfeI | AGCGCT | 1 cut(s) | 173 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 52, 635 |
| AflII | CTTAAG | 1 cut(s) | 734 |
| AflIII | ACRYGT | 1 cut(s) | 139 |
| AgsI | TTSAA | 5 cut(s) | 187, 307, 343, 483, 804 |
| AjiI | CACGTC | 1 cut(s) | 140 |
| AluBI | AGCT | 2 cut(s) | 368, 395 |
| AluI | AGCT | 2 cut(s) | 368, 395 |
| Alw21I | GWGCWC | 1 cut(s) | 730 |
| Alw26I | GTCTC | 2 cut(s) | 89, 611 |
| Alw44I | GTGCAC | 1 cut(s) | 726 |
| AlwI | GGATC | 2 cut(s) | 37, 790 |
| Aor51HI | AGCGCT | 1 cut(s) | 173 |
| AoxI | GGCC | 3 cut(s) | 36, 418, 694 |
| ApaLI | GTGCAC | 1 cut(s) | 726 |
| ApeKI | GCWGC | 3 cut(s) | 455, 680, 965 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 234, 266 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 173 |
| AspLEI | GCGC | 1 cut(s) | 174 |
| AspS9I | GGNCC | 1 cut(s) | 412 |
| AsuHPI | GGTGA | 3 cut(s) | 551, 913, 941 |
| AvaII | GGWCC | 1 cut(s) | 412 |
| BaeGI | GKGCMC | 1 cut(s) | 730 |
| BalI | TGGCCA | 1 cut(s) | 696 |
| BbsI | GAAGAC | 2 cut(s) | 54, 549 |
| Bbv12I | GWGCWC | 1 cut(s) | 730 |
| BbvI | GCAGC | 3 cut(s) | 467, 692, 952 |
| BccI | CCATC | 5 cut(s) | 118, 413, 562, 638, 875 |
| BcoDI | GTCTC | 2 cut(s) | 89, 611 |
| BfaI | CTAG | 2 cut(s) | 777, 839 |
| BfoI | RGCGCY | 1 cut(s) | 175 |
| BfrI | CTTAAG | 1 cut(s) | 734 |
| BisI | GCNGC | 3 cut(s) | 456, 681, 966 |
| BlsI | GCNGC | 3 cut(s) | 457, 682, 967 |
| Bme18I | GGWCC | 1 cut(s) | 412 |
| BmgBI | CACGTC | 1 cut(s) | 140 |
| BmgT120I | GGNCC | 1 cut(s) | 412 |
| BmsI | GCATC | 3 cut(s) | 419, 565, 763 |
| BpiI | GAAGAC | 2 cut(s) | 54, 549 |
| BpmI | CTGGAG | 1 cut(s) | 400 |
| BpuEI | CTTGAG | 1 cut(s) | 277 |
| Bsa29I | ATCGAT | 1 cut(s) | 301 |
| BsaHI | GRCGYC | 1 cut(s) | 870 |
| BsaJI | CCNNGG | 3 cut(s) | 415, 459, 547 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 52, 635 |
| Bse1I | ACTGG | 4 cut(s) | 383, 490, 640, 697 |
| Bse3DI | GCAATG | 2 cut(s) | 153, 661 |
| BseCI | ATCGAT | 1 cut(s) | 301 |
| BseDI | CCNNGG | 3 cut(s) | 415, 459, 547 |
| BseGI | GGATG | 3 cut(s) | 129, 434, 577 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 52, 635 |
| BseMI | GCAATG | 2 cut(s) | 153, 661 |
| BseMII | CTCAG | 1 cut(s) | 459 |
| BseNI | ACTGG | 4 cut(s) | 383, 490, 640, 697 |
| BseSI | GKGCMC | 1 cut(s) | 730 |
| BseXI | GCAGC | 3 cut(s) | 467, 692, 952 |
| BsgI | GTGCAG | 1 cut(s) | 951 |
| BshFI | GGCC | 3 cut(s) | 38, 420, 696 |
| BshVI | ATCGAT | 1 cut(s) | 301 |
| BsiHKAI | GWGCWC | 1 cut(s) | 730 |
| BslFI | GGGAC | 1 cut(s) | 898 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 52, 635 |
| BsmAI | GTCTC | 2 cut(s) | 89, 611 |
| BsmFI | GGGAC | 1 cut(s) | 898 |
| BsmI | GAATGC | 1 cut(s) | 954 |
| BsnI | GGCC | 3 cut(s) | 38, 420, 696 |
| Bsp1286I | GDGCHC | 1 cut(s) | 730 |
| Bsp143I | GATC | 3 cut(s) | 42, 782, 841 |
| Bsp19I | CCATGG | 1 cut(s) | 459 |
| BspACI | CCGC | 2 cut(s) | 79, 855 |
| BspANI | GGCC | 3 cut(s) | 38, 420, 696 |
| BspCNI | CTCAG | 1 cut(s) | 458 |
| BspDI | ATCGAT | 1 cut(s) | 301 |
| BspPI | GGATC | 2 cut(s) | 37, 790 |
| BspTI | CTTAAG | 1 cut(s) | 734 |
| BsrDI | GCAATG | 2 cut(s) | 153, 661 |
| BsrI | ACTGG | 4 cut(s) | 383, 490, 640, 697 |
| BssECI | CCNNGG | 3 cut(s) | 415, 459, 547 |
| BssMI | GATC | 3 cut(s) | 42, 782, 841 |
| BssNAI | GTATAC | 1 cut(s) | 145 |
| BssNI | GRCGYC | 1 cut(s) | 870 |
| BssT1I | CCWWGG | 3 cut(s) | 415, 459, 547 |
| Bst1107I | GTATAC | 1 cut(s) | 145 |
| Bst4CI | ACNGT | 4 cut(s) | 149, 361, 527, 719 |
| Bst6I | CTCTTC | 1 cut(s) | 273 |
| BstACI | GRCGYC | 1 cut(s) | 870 |
| BstAFI | CTTAAG | 1 cut(s) | 734 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 677 |
| BstC8I | GCNNGC | 2 cut(s) | 366, 711 |
| BstDEI | CTNAG | 3 cut(s) | 445, 608, 888 |
| BstDSI | CCRYGG | 1 cut(s) | 459 |
| BstF5I | GGATG | 3 cut(s) | 129, 434, 577 |
| BstH2I | RGCGCY | 1 cut(s) | 175 |
| BstHHI | GCGC | 1 cut(s) | 174 |
| BstKTI | GATC | 3 cut(s) | 45, 785, 844 |
| BstMAI | GTCTC | 2 cut(s) | 89, 611 |
| BstMBI | GATC | 3 cut(s) | 42, 782, 841 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 446, 455, 677, 773, 998 |
| BstNSI | RCATGY | 1 cut(s) | 654 |
| BstSLI | GKGCMC | 1 cut(s) | 730 |
| BstV1I | GCAGC | 3 cut(s) | 467, 692, 952 |
| BstV2I | GAAGAC | 2 cut(s) | 54, 549 |
| BstX2I | RGATCY | 1 cut(s) | 782 |
| BstYI | RGATCY | 1 cut(s) | 782 |
| BstZ17I | GTATAC | 1 cut(s) | 145 |
| Bsu15I | ATCGAT | 1 cut(s) | 301 |
| BsuRI | GGCC | 3 cut(s) | 38, 420, 696 |
| BsuTUI | ATCGAT | 1 cut(s) | 301 |
| BtgI | CCRYGG | 1 cut(s) | 459 |
| BtrI | CACGTC | 1 cut(s) | 140 |
| BtsCI | GGATG | 3 cut(s) | 129, 434, 577 |
| BtsI | GCAGTG | 3 cut(s) | 102, 456, 1011 |
| BtsIMutI | CAGTG | 6 cut(s) | 102, 154, 456, 724, 880, 1011 |
| Cac8I | GCNNGC | 2 cut(s) | 366, 711 |
| CfoI | GCGC | 1 cut(s) | 174 |
| Cfr13I | GGNCC | 1 cut(s) | 412 |
| ClaI | ATCGAT | 1 cut(s) | 301 |
| CseI | GACGC | 2 cut(s) | 101, 859 |
| Csp6I | GTAC | 2 cut(s) | 648, 940 |
| CviAII | CATG | 9 cut(s) | 49, 314, 460, 602, 651, 677, 698, 845, 912 |
| CviQI | GTAC | 2 cut(s) | 648, 940 |
| DdeI | CTNAG | 3 cut(s) | 445, 608, 888 |
| DpnI | GATC | 3 cut(s) | 44, 784, 843 |
| DpnII | GATC | 3 cut(s) | 42, 782, 841 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 530 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 530 |
| EaeI | YGGCCR | 1 cut(s) | 694 |
| Eam1104I | CTCTTC | 1 cut(s) | 273 |
| EarI | CTCTTC | 1 cut(s) | 273 |
| Eco130I | CCWWGG | 3 cut(s) | 415, 459, 547 |
| Eco47I | GGWCC | 1 cut(s) | 412 |
| Eco47III | AGCGCT | 1 cut(s) | 173 |
| Eco57I | CTGAAG | 1 cut(s) | 87 |
| EcoT14I | CCWWGG | 3 cut(s) | 415, 459, 547 |
| ErhI | CCWWGG | 3 cut(s) | 415, 459, 547 |
| FaeI | CATG | 9 cut(s) | 52, 317, 463, 605, 654, 680, 701, 848, 915 |
| FaqI | GGGAC | 1 cut(s) | 898 |
| FatI | CATG | 9 cut(s) | 48, 313, 459, 601, 650, 676, 697, 844, 911 |
| FauI | CCCGC | 1 cut(s) | 86 |
| FblI | GTMKAC | 1 cut(s) | 144 |
| Fnu4HI | GCNGC | 3 cut(s) | 456, 681, 966 |
| FokI | GGATG | 3 cut(s) | 136, 441, 584 |
| Fsp4HI | GCNGC | 3 cut(s) | 456, 681, 966 |
| FspBI | CTAG | 2 cut(s) | 777, 839 |
| GlaI | GCGC | 1 cut(s) | 173 |
| GluI | GCNGC | 3 cut(s) | 456, 681, 966 |
| GsuI | CTGGAG | 1 cut(s) | 400 |
| HaeII | RGCGCY | 1 cut(s) | 175 |
| HaeIII | GGCC | 3 cut(s) | 38, 420, 696 |
| HgaI | GACGC | 2 cut(s) | 101, 859 |
| HhaI | GCGC | 1 cut(s) | 174 |
| Hin1I | GRCGYC | 1 cut(s) | 870 |
| Hin1II | CATG | 9 cut(s) | 52, 317, 463, 605, 654, 680, 701, 848, 915 |
| Hin6I | GCGC | 1 cut(s) | 172 |
| HinP1I | GCGC | 1 cut(s) | 172 |
| HinfI | GANTC | 3 cut(s) | 209, 303, 400 |
| HphI | GGTGA | 3 cut(s) | 551, 913, 941 |
| Hpy166II | GTNNAC | 4 cut(s) | 145, 285, 523, 728 |
| Hpy188I | TCNGA | 3 cut(s) | 67, 508, 787 |
| Hpy188III | TCNNGA | 1 cut(s) | 294 |
| Hpy8I | GTNNAC | 4 cut(s) | 145, 285, 523, 728 |
| HpyAV | CCTTC | 5 cut(s) | 157, 458, 658, 929, 968 |
| HpyCH4III | ACNGT | 4 cut(s) | 149, 361, 527, 719 |
| HpyCH4IV | ACGT | 1 cut(s) | 139 |
| HpyCH4V | TGCA | 8 cut(s) | 21, 263, 654, 680, 713, 728, 952, 968 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 446, 455, 677, 773, 998 |
| HpyF3I | CTNAG | 3 cut(s) | 445, 608, 888 |
| HpySE526I | ACGT | 1 cut(s) | 139 |
| Hsp92I | GRCGYC | 1 cut(s) | 870 |
| Hsp92II | CATG | 9 cut(s) | 52, 317, 463, 605, 654, 680, 701, 848, 915 |
| HspAI | GCGC | 1 cut(s) | 172 |
| Kzo9I | GATC | 3 cut(s) | 42, 782, 841 |
| LmnI | GCTCC | 1 cut(s) | 392 |
| LpnPI | CCDG | 8 cut(s) | 364, 378, 471, 621, 678, 723, 804, 956 |
| Lsp1109I | GCAGC | 3 cut(s) | 467, 692, 952 |
| LweI | GCATC | 3 cut(s) | 419, 565, 763 |
| MaeI | CTAG | 2 cut(s) | 777, 839 |
| MaeII | ACGT | 1 cut(s) | 139 |
| MaeIII | GTNAC | 4 cut(s) | 149, 355, 397, 850 |
| MalI | GATC | 3 cut(s) | 44, 784, 843 |
| MboI | GATC | 3 cut(s) | 42, 782, 841 |
| MboII | GAAGA | 7 cut(s) | 54, 204, 290, 554, 713, 916, 944 |
| MflI | RGATCY | 1 cut(s) | 782 |
| MhlI | GDGCHC | 1 cut(s) | 730 |
| MlsI | TGGCCA | 1 cut(s) | 696 |
| MluCI | AATT | 5 cut(s) | 242, 468, 491, 622, 794 |
| MluNI | TGGCCA | 1 cut(s) | 696 |
| MlyI | GAGTC | 1 cut(s) | 394 |
| MnlI | CCTC | 7 cut(s) | 56, 301, 375, 550, 554, 694, 1012 |
| Mox20I | TGGCCA | 1 cut(s) | 696 |
| MroXI | GAANNNNTTC | 1 cut(s) | 173 |
| MscI | TGGCCA | 1 cut(s) | 696 |
| MseI | TTAA | 3 cut(s) | 245, 735, 1018 |
| MslI | CAYNNNNRTG | 2 cut(s) | 702, 947 |
| Msp20I | TGGCCA | 1 cut(s) | 696 |
| MspA1I | CMGCKG | 1 cut(s) | 857 |
| MspCI | CTTAAG | 1 cut(s) | 734 |
| Mva1269I | GAATGC | 1 cut(s) | 954 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 446, 455, 677, 773, 998 |
| NcoI | CCATGG | 1 cut(s) | 459 |
| NdeII | GATC | 3 cut(s) | 42, 782, 841 |
| NlaIII | CATG | 9 cut(s) | 52, 317, 463, 605, 654, 680, 701, 848, 915 |
| NmeAIII | GCCGAG | 1 cut(s) | 184 |
| NmuCI | GTSAC | 2 cut(s) | 149, 397 |
| NspI | RCATGY | 1 cut(s) | 654 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 136 |
| PctI | GAATGC | 1 cut(s) | 954 |
| PdmI | GAANNNNTTC | 1 cut(s) | 173 |
| PfeI | GAWTC | 2 cut(s) | 209, 303 |
| PkrI | GCNGC | 3 cut(s) | 457, 682, 967 |
| PleI | GAGTC | 1 cut(s) | 394 |
| PpsI | GAGTC | 1 cut(s) | 394 |
| PspPI | GGNCC | 1 cut(s) | 412 |
| PsuI | RGATCY | 1 cut(s) | 782 |
| RsaI | GTAC | 2 cut(s) | 649, 941 |
| RsaNI | GTAC | 2 cut(s) | 648, 940 |
| RseI | CAYNNNNRTG | 2 cut(s) | 702, 947 |
| SaqAI | TTAA | 3 cut(s) | 245, 735, 1018 |
| SatI | GCNGC | 3 cut(s) | 456, 681, 966 |
| Sau3AI | GATC | 3 cut(s) | 42, 782, 841 |
| Sau96I | GGNCC | 1 cut(s) | 412 |
| SchI | GAGTC | 1 cut(s) | 394 |
| SduI | GDGCHC | 1 cut(s) | 730 |
| SetI | ASST | 7 cut(s) | 142, 238, 270, 370, 397, 757, 979 |
| SfaNI | GCATC | 3 cut(s) | 419, 565, 763 |
| SinI | GGWCC | 1 cut(s) | 412 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 702, 947 |
| SmlI | CTYRAG | 2 cut(s) | 292, 734 |
| SmoI | CTYRAG | 2 cut(s) | 292, 734 |
| Sse9I | AATT | 5 cut(s) | 242, 468, 491, 622, 794 |
| SsiI | CCGC | 2 cut(s) | 79, 855 |
| SspI | AATATT | 1 cut(s) | 183 |
| SspMI | CTAG | 2 cut(s) | 777, 839 |
| StyI | CCWWGG | 3 cut(s) | 415, 459, 547 |
| TaaI | ACNGT | 4 cut(s) | 149, 361, 527, 719 |
| TaiI | ACGT | 1 cut(s) | 142 |
| TaqI | TCGA | 2 cut(s) | 301, 686 |
| TasI | AATT | 5 cut(s) | 242, 468, 491, 622, 794 |
| TfiI | GAWTC | 2 cut(s) | 209, 303 |
| Tru1I | TTAA | 3 cut(s) | 245, 735, 1018 |
| Tru9I | TTAA | 3 cut(s) | 245, 735, 1018 |
| TscAI | CASTG | 6 cut(s) | 102, 154, 456, 724, 880, 1018 |
| TseFI | GTSAC | 2 cut(s) | 149, 397 |
| TseI | GCWGC | 3 cut(s) | 455, 680, 965 |
| Tsp45I | GTSAC | 2 cut(s) | 149, 397 |
| TspDTI | ATGAA | 5 cut(s) | 291, 330, 363, 577, 714 |
| TspRI | CASTG | 6 cut(s) | 102, 154, 456, 724, 880, 1018 |
| Vha464I | CTTAAG | 1 cut(s) | 734 |
| VneI | GTGCAC | 1 cut(s) | 726 |
| VpaK11BI | GGWCC | 1 cut(s) | 412 |
| XceI | RCATGY | 1 cut(s) | 654 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 422 |
| XmiI | GTMKAC | 1 cut(s) | 144 |
| XmnI | GAANNNNTTC | 1 cut(s) | 173 |
| XspI | CTAG | 2 cut(s) | 777, 839 |