Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 355 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 894 |
| AccII | CGCG | 1 cut(s) | 135 |
| AciI | CCGC | 4 cut(s) | 537, 614, 738, 855 |
| AclWI | GGATC | 2 cut(s) | 816, 829 |
| AcyI | GRCGYC | 1 cut(s) | 356 |
| AfaI | GTAC | 2 cut(s) | 98, 941 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 409, 635, 894 |
| AgsI | TTSAA | 6 cut(s) | 181, 280, 301, 337, 804, 965 |
| AjnI | CCWGG | 1 cut(s) | 414 |
| AluBI | AGCT | 6 cut(s) | 50, 368, 395, 478, 517, 620 |
| AluI | AGCT | 6 cut(s) | 50, 368, 395, 478, 517, 620 |
| Alw26I | GTCTC | 1 cut(s) | 83 |
| AlwI | GGATC | 2 cut(s) | 816, 829 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 761 |
| AoxI | GGCC | 1 cut(s) | 418 |
| ApeKI | GCWGC | 3 cut(s) | 455, 668, 680 |
| AspLEI | GCGC | 1 cut(s) | 358 |
| AspS9I | GGNCC | 3 cut(s) | 412, 418, 885 |
| AsuHPI | GGTGA | 2 cut(s) | 551, 941 |
| AvaII | GGWCC | 2 cut(s) | 412, 885 |
| BamHI | GGATCC | 1 cut(s) | 821 |
| BanI | GGYRCC | 1 cut(s) | 355 |
| BbsI | GAAGAC | 1 cut(s) | 549 |
| BbvI | GCAGC | 3 cut(s) | 467, 680, 692 |
| BccI | CCATC | 3 cut(s) | 367, 562, 875 |
| BciT130I | CCWGG | 1 cut(s) | 416 |
| BcoDI | GTCTC | 1 cut(s) | 83 |
| BfaI | CTAG | 1 cut(s) | 518 |
| BfoI | RGCGCY | 1 cut(s) | 359 |
| BisI | GCNGC | 3 cut(s) | 456, 669, 681 |
| BlsI | GCNGC | 3 cut(s) | 457, 670, 682 |
| Bme1390I | CCNGG | 1 cut(s) | 416 |
| Bme18I | GGWCC | 2 cut(s) | 412, 885 |
| BmgT120I | GGNCC | 3 cut(s) | 412, 418, 885 |
| BmiI | GGNNCC | 5 cut(s) | 6, 357, 784, 823, 886 |
| BmrFI | CCNGG | 1 cut(s) | 416 |
| BmsI | GCATC | 5 cut(s) | 76, 98, 565, 763, 939 |
| BpiI | GAAGAC | 1 cut(s) | 549 |
| BpmI | CTGGAG | 2 cut(s) | 400, 798 |
| BpuEI | CTTGAG | 1 cut(s) | 271 |
| BsaHI | GRCGYC | 1 cut(s) | 356 |
| BsaJI | CCNNGG | 5 cut(s) | 8, 415, 547, 601, 844 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 409, 635, 894 |
| Bse1I | ACTGG | 6 cut(s) | 359, 383, 490, 697, 755, 793 |
| Bse3DI | GCAATG | 3 cut(s) | 119, 675, 987 |
| BseBI | CCWGG | 1 cut(s) | 416 |
| BseDI | CCNNGG | 5 cut(s) | 8, 415, 547, 601, 844 |
| BseGI | GGATG | 6 cut(s) | 123, 206, 434, 577, 588, 954 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 409, 635, 894 |
| BseMI | GCAATG | 3 cut(s) | 119, 675, 987 |
| BseMII | CTCAG | 2 cut(s) | 488, 498 |
| BseNI | ACTGG | 6 cut(s) | 359, 383, 490, 697, 755, 793 |
| BseXI | GCAGC | 3 cut(s) | 467, 680, 692 |
| Bsh1236I | CGCG | 1 cut(s) | 135 |
| BshFI | GGCC | 1 cut(s) | 420 |
| BshNI | GGYRCC | 1 cut(s) | 355 |
| BslFI | GGGAC | 2 cut(s) | 898, 931 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 409, 635, 894 |
| BsmAI | GTCTC | 1 cut(s) | 83 |
| BsmFI | GGGAC | 2 cut(s) | 898, 931 |
| BsnI | GGCC | 1 cut(s) | 420 |
| Bsp143I | GATC | 2 cut(s) | 495, 821 |
| Bsp19I | CCATGG | 3 cut(s) | 8, 601, 844 |
| BspACI | CCGC | 4 cut(s) | 537, 614, 738, 855 |
| BspANI | GGCC | 1 cut(s) | 420 |
| BspCNI | CTCAG | 2 cut(s) | 487, 499 |
| BspFNI | CGCG | 1 cut(s) | 135 |
| BspLI | GGNNCC | 5 cut(s) | 6, 357, 784, 823, 886 |
| BspPI | GGATC | 2 cut(s) | 816, 829 |
| BspQI | GCTCTTC | 1 cut(s) | 41 |
| BspT107I | GGYRCC | 1 cut(s) | 355 |
| BsrDI | GCAATG | 3 cut(s) | 119, 675, 987 |
| BsrI | ACTGG | 6 cut(s) | 359, 383, 490, 697, 755, 793 |
| BssECI | CCNNGG | 5 cut(s) | 8, 415, 547, 601, 844 |
| BssMI | GATC | 2 cut(s) | 495, 821 |
| BssNI | GRCGYC | 1 cut(s) | 356 |
| BssT1I | CCWWGG | 4 cut(s) | 8, 547, 601, 844 |
| Bst2UI | CCWGG | 1 cut(s) | 416 |
| Bst4CI | ACNGT | 2 cut(s) | 527, 647 |
| Bst6I | CTCTTC | 3 cut(s) | 41, 267, 927 |
| BstACI | GRCGYC | 1 cut(s) | 356 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 677 |
| BstC8I | GCNNGC | 4 cut(s) | 32, 366, 673, 711 |
| BstDEI | CTNAG | 4 cut(s) | 474, 507, 608, 1017 |
| BstDSI | CCRYGG | 3 cut(s) | 8, 601, 844 |
| BstEII | GGTNACC | 1 cut(s) | 850 |
| BstF5I | GGATG | 6 cut(s) | 123, 206, 434, 577, 588, 954 |
| BstFNI | CGCG | 1 cut(s) | 135 |
| BstH2I | RGCGCY | 1 cut(s) | 359 |
| BstHHI | GCGC | 1 cut(s) | 358 |
| BstKTI | GATC | 2 cut(s) | 498, 824 |
| BstMAI | GTCTC | 1 cut(s) | 83 |
| BstMBI | GATC | 2 cut(s) | 495, 821 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 446, 455, 677, 773, 998 |
| BstNI | CCWGG | 1 cut(s) | 416 |
| BstPI | GGTNACC | 1 cut(s) | 850 |
| BstSCI | CCNGG | 1 cut(s) | 414 |
| BstUI | CGCG | 1 cut(s) | 135 |
| BstV1I | GCAGC | 3 cut(s) | 467, 680, 692 |
| BstV2I | GAAGAC | 1 cut(s) | 549 |
| BstX2I | RGATCY | 1 cut(s) | 821 |
| BstYI | RGATCY | 1 cut(s) | 821 |
| BsuRI | GGCC | 1 cut(s) | 420 |
| BtgI | CCRYGG | 3 cut(s) | 8, 601, 844 |
| BtgZI | GCGATG | 1 cut(s) | 891 |
| BtsCI | GGATG | 6 cut(s) | 123, 206, 434, 577, 588, 954 |
| BtsI | GCAGTG | 2 cut(s) | 456, 1011 |
| BtsIMutI | CAGTG | 3 cut(s) | 366, 456, 1011 |
| Cac8I | GCNNGC | 4 cut(s) | 32, 366, 673, 711 |
| CaiI | CAGNNNCTG | 1 cut(s) | 761 |
| CfoI | GCGC | 1 cut(s) | 358 |
| Cfr13I | GGNCC | 3 cut(s) | 412, 418, 885 |
| Csp6I | GTAC | 2 cut(s) | 97, 940 |
| CviAII | CATG | 7 cut(s) | 9, 40, 308, 602, 651, 845, 912 |
| CviQI | GTAC | 2 cut(s) | 97, 940 |
| DdeI | CTNAG | 4 cut(s) | 474, 507, 608, 1017 |
| DinI | GGCGCC | 1 cut(s) | 357 |
| DpnI | GATC | 2 cut(s) | 497, 823 |
| DpnII | GATC | 2 cut(s) | 495, 821 |
| Eam1104I | CTCTTC | 3 cut(s) | 41, 267, 927 |
| EarI | CTCTTC | 3 cut(s) | 41, 267, 927 |
| Eco130I | CCWWGG | 4 cut(s) | 8, 547, 601, 844 |
| Eco47I | GGWCC | 2 cut(s) | 412, 885 |
| Eco91I | GGTNACC | 1 cut(s) | 850 |
| EcoO65I | GGTNACC | 1 cut(s) | 850 |
| EcoRII | CCWGG | 1 cut(s) | 414 |
| EcoT14I | CCWWGG | 4 cut(s) | 8, 547, 601, 844 |
| EcoT22I | ATGCAT | 1 cut(s) | 91 |
| EgeI | GGCGCC | 1 cut(s) | 357 |
| EheI | GGCGCC | 1 cut(s) | 357 |
| ErhI | CCWWGG | 4 cut(s) | 8, 547, 601, 844 |
| FaeI | CATG | 7 cut(s) | 12, 43, 311, 605, 654, 848, 915 |
| FaqI | GGGAC | 2 cut(s) | 898, 931 |
| FatI | CATG | 7 cut(s) | 8, 39, 307, 601, 650, 844, 911 |
| Fnu4HI | GCNGC | 3 cut(s) | 456, 669, 681 |
| FokI | GGATG | 6 cut(s) | 130, 193, 441, 575, 584, 961 |
| Fsp4HI | GCNGC | 3 cut(s) | 456, 669, 681 |
| FspBI | CTAG | 1 cut(s) | 518 |
| GlaI | GCGC | 1 cut(s) | 357 |
| GluI | GCNGC | 3 cut(s) | 456, 669, 681 |
| GsuI | CTGGAG | 2 cut(s) | 400, 798 |
| HaeII | RGCGCY | 1 cut(s) | 359 |
| HaeIII | GGCC | 1 cut(s) | 420 |
| HhaI | GCGC | 1 cut(s) | 358 |
| Hin1I | GRCGYC | 1 cut(s) | 356 |
| Hin1II | CATG | 7 cut(s) | 12, 43, 311, 605, 654, 848, 915 |
| Hin6I | GCGC | 1 cut(s) | 356 |
| HinP1I | GCGC | 1 cut(s) | 356 |
| HincII | GTYRAC | 3 cut(s) | 151, 523, 643 |
| HindII | GTYRAC | 3 cut(s) | 151, 523, 643 |
| HinfI | GANTC | 4 cut(s) | 121, 163, 203, 297 |
| HpaI | GTTAAC | 1 cut(s) | 151 |
| HphI | GGTGA | 2 cut(s) | 551, 941 |
| Hpy166II | GTNNAC | 5 cut(s) | 151, 223, 491, 523, 643 |
| Hpy188I | TCNGA | 2 cut(s) | 508, 787 |
| Hpy188III | TCNNGA | 1 cut(s) | 288 |
| Hpy8I | GTNNAC | 5 cut(s) | 151, 223, 491, 523, 643 |
| Hpy99I | CGWCG | 2 cut(s) | 149, 463 |
| HpyAV | CCTTC | 4 cut(s) | 151, 458, 658, 968 |
| HpyCH4III | ACNGT | 2 cut(s) | 527, 647 |
| HpyCH4IV | ACGT | 1 cut(s) | 147 |
| HpyCH4V | TGCA | 6 cut(s) | 89, 257, 654, 680, 713, 952 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 446, 455, 677, 773, 998 |
| HpyF3I | CTNAG | 4 cut(s) | 474, 507, 608, 1017 |
| HpySE526I | ACGT | 1 cut(s) | 147 |
| Hsp92I | GRCGYC | 1 cut(s) | 356 |
| Hsp92II | CATG | 7 cut(s) | 12, 43, 311, 605, 654, 848, 915 |
| HspAI | GCGC | 1 cut(s) | 356 |
| KasI | GGCGCC | 1 cut(s) | 355 |
| KspAI | GTTAAC | 1 cut(s) | 151 |
| Kzo9I | GATC | 2 cut(s) | 495, 821 |
| LguI | GCTCTTC | 1 cut(s) | 41 |
| LmnI | GCTCC | 3 cut(s) | 4, 47, 392 |
| Lsp1109I | GCAGC | 3 cut(s) | 467, 680, 692 |
| LweI | GCATC | 5 cut(s) | 76, 98, 565, 763, 939 |
| MaeI | CTAG | 1 cut(s) | 518 |
| MaeII | ACGT | 1 cut(s) | 147 |
| MaeIII | GTNAC | 3 cut(s) | 397, 527, 850 |
| MalI | GATC | 2 cut(s) | 497, 823 |
| MboI | GATC | 2 cut(s) | 495, 821 |
| MboII | GAAGA | 5 cut(s) | 28, 284, 554, 713, 944 |
| MflI | RGATCY | 1 cut(s) | 821 |
| MluCI | AATT | 2 cut(s) | 251, 622 |
| Mly113I | GGCGCC | 1 cut(s) | 356 |
| MlyI | GAGTC | 1 cut(s) | 115 |
| MmeI | TCCRAC | 1 cut(s) | 616 |
| MnlI | CCTC | 6 cut(s) | 79, 295, 550, 773, 928, 1012 |
| Mph1103I | ATGCAT | 1 cut(s) | 91 |
| MseI | TTAA | 5 cut(s) | 150, 171, 246, 351, 903 |
| MslI | CAYNNNNRTG | 3 cut(s) | 13, 947, 992 |
| MspA1I | CMGCKG | 3 cut(s) | 537, 740, 857 |
| MspR9I | CCNGG | 1 cut(s) | 416 |
| MvaI | CCWGG | 1 cut(s) | 416 |
| MvnI | CGCG | 1 cut(s) | 135 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 446, 455, 677, 773, 998 |
| NarI | GGCGCC | 1 cut(s) | 356 |
| NcoI | CCATGG | 3 cut(s) | 8, 601, 844 |
| NdeII | GATC | 2 cut(s) | 495, 821 |
| NlaIII | CATG | 7 cut(s) | 12, 43, 311, 605, 654, 848, 915 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 357, 784, 823, 886 |
| NmuCI | GTSAC | 1 cut(s) | 527 |
| NsiI | ATGCAT | 1 cut(s) | 91 |
| PciSI | GCTCTTC | 1 cut(s) | 41 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 105, 150 |
| PfeI | GAWTC | 3 cut(s) | 163, 203, 297 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 894 |
| PkrI | GCNGC | 3 cut(s) | 457, 670, 682 |
| PleI | GAGTC | 1 cut(s) | 115 |
| PluTI | GGCGCC | 1 cut(s) | 359 |
| PpsI | GAGTC | 1 cut(s) | 115 |
| Psp6I | CCWGG | 1 cut(s) | 414 |
| PspEI | GGTNACC | 1 cut(s) | 850 |
| PspGI | CCWGG | 1 cut(s) | 414 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 357, 784, 823, 886 |
| PspPI | GGNCC | 3 cut(s) | 412, 418, 885 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 215, 247 |
| PstNI | CAGNNNCTG | 1 cut(s) | 761 |
| PsuI | RGATCY | 1 cut(s) | 821 |
| RsaI | GTAC | 2 cut(s) | 98, 941 |
| RsaNI | GTAC | 2 cut(s) | 97, 940 |
| RseI | CAYNNNNRTG | 3 cut(s) | 13, 947, 992 |
| SapI | GCTCTTC | 1 cut(s) | 41 |
| SaqAI | TTAA | 5 cut(s) | 150, 171, 246, 351, 903 |
| SatI | GCNGC | 3 cut(s) | 456, 669, 681 |
| Sau3AI | GATC | 2 cut(s) | 495, 821 |
| Sau96I | GGNCC | 3 cut(s) | 412, 418, 885 |
| SchI | GAGTC | 1 cut(s) | 115 |
| ScrFI | CCNGG | 1 cut(s) | 416 |
| SfaNI | GCATC | 5 cut(s) | 76, 98, 565, 763, 939 |
| SfoI | GGCGCC | 1 cut(s) | 357 |
| SinI | GGWCC | 2 cut(s) | 412, 885 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 13, 947, 992 |
| SmlI | CTYRAG | 1 cut(s) | 286 |
| SmoI | CTYRAG | 1 cut(s) | 286 |
| Sse9I | AATT | 2 cut(s) | 251, 622 |
| SsiI | CCGC | 4 cut(s) | 537, 614, 738, 855 |
| SspDI | GGCGCC | 1 cut(s) | 355 |
| SspMI | CTAG | 1 cut(s) | 518 |
| StyD4I | CCNGG | 1 cut(s) | 414 |
| StyI | CCWWGG | 4 cut(s) | 8, 547, 601, 844 |
| TaaI | ACNGT | 2 cut(s) | 527, 647 |
| TaiI | ACGT | 1 cut(s) | 150 |
| TasI | AATT | 2 cut(s) | 251, 622 |
| TfiI | GAWTC | 3 cut(s) | 163, 203, 297 |
| Tru1I | TTAA | 5 cut(s) | 150, 171, 246, 351, 903 |
| Tru9I | TTAA | 5 cut(s) | 150, 171, 246, 351, 903 |
| TscAI | CASTG | 3 cut(s) | 366, 456, 1018 |
| TseFI | GTSAC | 1 cut(s) | 527 |
| TseI | GCWGC | 3 cut(s) | 455, 668, 680 |
| Tsp45I | GTSAC | 1 cut(s) | 527 |
| TspDTI | ATGAA | 6 cut(s) | 28, 285, 324, 577, 639, 714 |
| TspGWI | ACGGA | 1 cut(s) | 476 |
| TspRI | CASTG | 3 cut(s) | 366, 456, 1018 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 894 |
| VpaK11BI | GGWCC | 2 cut(s) | 412, 885 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 422, 599, 895 |
| XspI | CTAG | 1 cut(s) | 518 |
| Zsp2I | ATGCAT | 1 cut(s) | 91 |