Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 584 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 387 |
| AclWI | GGATC | 3 cut(s) | 40, 832, 1012 |
| AcyI | GRCGYC | 1 cut(s) | 585 |
| AfaI | GTAC | 3 cut(s) | 110, 372, 944 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 55, 387, 739 |
| AflII | CTTAAG | 1 cut(s) | 737 |
| AgsI | TTSAA | 4 cut(s) | 193, 313, 349, 473 |
| AhlI | ACTAGT | 1 cut(s) | 494 |
| AjiI | CACGTC | 1 cut(s) | 146 |
| AleI | CACNNNNGTG | 1 cut(s) | 647 |
| AluBI | AGCT | 5 cut(s) | 19, 404, 441, 621, 995 |
| AluI | AGCT | 5 cut(s) | 19, 404, 441, 621, 995 |
| AlwI | GGATC | 3 cut(s) | 40, 832, 1012 |
| AoxI | GGCC | 1 cut(s) | 427 |
| ApeKI | GCWGC | 4 cut(s) | 16, 455, 464, 683 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 572, 604 |
| AseI | ATTAAT | 1 cut(s) | 503 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 732 |
| AspA2I | CCTAGG | 1 cut(s) | 556 |
| AspLEI | GCGC | 1 cut(s) | 587 |
| AsuHPI | GGTGA | 1 cut(s) | 944 |
| AvrII | CCTAGG | 1 cut(s) | 556 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 354, 387 |
| BanI | GGYRCC | 1 cut(s) | 584 |
| BbvI | GCAGC | 4 cut(s) | 28, 467, 476, 695 |
| BccI | CCATC | 4 cut(s) | 124, 382, 878, 1022 |
| BceAI | ACGGC | 3 cut(s) | 452, 562, 598 |
| BcuI | ACTAGT | 1 cut(s) | 494 |
| BfaI | CTAG | 5 cut(s) | 69, 495, 557, 630, 836 |
| BfoI | RGCGCY | 1 cut(s) | 588 |
| BfrI | CTTAAG | 1 cut(s) | 737 |
| BisI | GCNGC | 4 cut(s) | 17, 456, 465, 684 |
| BlnI | CCTAGG | 1 cut(s) | 556 |
| BlsI | GCNGC | 4 cut(s) | 18, 457, 466, 685 |
| BmgBI | CACGTC | 1 cut(s) | 146 |
| BmiI | GGNNCC | 1 cut(s) | 586 |
| BmsI | GCATC | 5 cut(s) | 88, 110, 650, 766, 942 |
| BpmI | CTGGAG | 1 cut(s) | 801 |
| BpuEI | CTTGAG | 1 cut(s) | 283 |
| Bsa29I | ATCGAT | 1 cut(s) | 843 |
| BsaHI | GRCGYC | 1 cut(s) | 585 |
| BsaJI | CCNNGG | 5 cut(s) | 381, 424, 556, 610, 624 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 55, 387, 739 |
| Bse1I | ACTGG | 1 cut(s) | 700 |
| Bse3DI | GCAATG | 1 cut(s) | 847 |
| BseCI | ATCGAT | 1 cut(s) | 843 |
| BseDI | CCNNGG | 5 cut(s) | 381, 424, 556, 610, 624 |
| BseGI | GGATG | 3 cut(s) | 135, 566, 957 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 55, 387, 739 |
| BseMI | GCAATG | 1 cut(s) | 847 |
| BseNI | ACTGG | 1 cut(s) | 700 |
| BseXI | GCAGC | 4 cut(s) | 28, 467, 476, 695 |
| Bsh1285I | CGRYCG | 1 cut(s) | 864 |
| BshFI | GGCC | 1 cut(s) | 429 |
| BshNI | GGYRCC | 1 cut(s) | 584 |
| BshVI | ATCGAT | 1 cut(s) | 843 |
| BsiEI | CGRYCG | 1 cut(s) | 864 |
| BslFI | GGGAC | 1 cut(s) | 73 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 55, 387, 739 |
| BsmFI | GGGAC | 1 cut(s) | 73 |
| BsnI | GGCC | 1 cut(s) | 429 |
| Bsp143I | GATC | 5 cut(s) | 45, 824, 844, 861, 1004 |
| Bsp19I | CCATGG | 1 cut(s) | 381 |
| BspANI | GGCC | 1 cut(s) | 429 |
| BspDI | ATCGAT | 1 cut(s) | 843 |
| BspHI | TCATGA | 1 cut(s) | 795 |
| BspLI | GGNNCC | 1 cut(s) | 586 |
| BspPI | GGATC | 3 cut(s) | 40, 832, 1012 |
| BspT107I | GGYRCC | 1 cut(s) | 584 |
| BspTI | CTTAAG | 1 cut(s) | 737 |
| BsrDI | GCAATG | 1 cut(s) | 847 |
| BsrI | ACTGG | 1 cut(s) | 700 |
| BssECI | CCNNGG | 5 cut(s) | 381, 424, 556, 610, 624 |
| BssMI | GATC | 5 cut(s) | 45, 824, 844, 861, 1004 |
| BssNI | GRCGYC | 1 cut(s) | 585 |
| BssT1I | CCWWGG | 4 cut(s) | 381, 424, 556, 610 |
| Bst4CI | ACNGT | 3 cut(s) | 367, 656, 872 |
| BstACI | GRCGYC | 1 cut(s) | 585 |
| BstAFI | CTTAAG | 1 cut(s) | 737 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 680 |
| BstC8I | GCNNGC | 2 cut(s) | 714, 718 |
| BstDSI | CCRYGG | 1 cut(s) | 381 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 737 |
| BstF5I | GGATG | 3 cut(s) | 135, 566, 957 |
| BstH2I | RGCGCY | 1 cut(s) | 588 |
| BstHHI | GCGC | 1 cut(s) | 587 |
| BstKTI | GATC | 5 cut(s) | 48, 827, 847, 864, 1007 |
| BstMBI | GATC | 5 cut(s) | 45, 824, 844, 861, 1004 |
| BstMCI | CGRYCG | 1 cut(s) | 864 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 455, 461, 464, 680 |
| BstV1I | GCAGC | 4 cut(s) | 28, 467, 476, 695 |
| BstX2I | RGATCY | 1 cut(s) | 1004 |
| BstYI | RGATCY | 1 cut(s) | 1004 |
| Bsu15I | ATCGAT | 1 cut(s) | 843 |
| BsuRI | GGCC | 1 cut(s) | 429 |
| BsuTUI | ATCGAT | 1 cut(s) | 843 |
| BtgI | CCRYGG | 1 cut(s) | 381 |
| BtgZI | GCGATG | 2 cut(s) | 243, 1000 |
| BtrI | CACGTC | 1 cut(s) | 146 |
| BtsCI | GGATG | 3 cut(s) | 135, 566, 957 |
| BtsI | GCAGTG | 1 cut(s) | 465 |
| BtsIMutI | CAGTG | 2 cut(s) | 465, 883 |
| Cac8I | GCNNGC | 2 cut(s) | 714, 718 |
| CciI | TCATGA | 1 cut(s) | 795 |
| CfoI | GCGC | 1 cut(s) | 587 |
| ClaI | ATCGAT | 1 cut(s) | 843 |
| Csp6I | GTAC | 3 cut(s) | 109, 371, 943 |
| CviAII | CATG | 6 cut(s) | 382, 680, 796, 828, 915, 946 |
| CviQI | GTAC | 3 cut(s) | 109, 371, 943 |
| DinI | GGCGCC | 1 cut(s) | 586 |
| DpnI | GATC | 5 cut(s) | 47, 826, 846, 863, 1006 |
| DpnII | GATC | 5 cut(s) | 45, 824, 844, 861, 1004 |
| Eco130I | CCWWGG | 4 cut(s) | 381, 424, 556, 610 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 737 |
| EcoT14I | CCWWGG | 4 cut(s) | 381, 424, 556, 610 |
| EcoT22I | ATGCAT | 1 cut(s) | 103 |
| EgeI | GGCGCC | 1 cut(s) | 586 |
| EheI | GGCGCC | 1 cut(s) | 586 |
| ErhI | CCWWGG | 4 cut(s) | 381, 424, 556, 610 |
| FaeI | CATG | 6 cut(s) | 385, 683, 799, 831, 918, 949 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 720, 752, 966, 998 |
| FaqI | GGGAC | 1 cut(s) | 73 |
| FatI | CATG | 6 cut(s) | 381, 679, 795, 827, 914, 945 |
| Fnu4HI | GCNGC | 4 cut(s) | 17, 456, 465, 684 |
| FokI | GGATG | 3 cut(s) | 142, 573, 964 |
| Fsp4HI | GCNGC | 4 cut(s) | 17, 456, 465, 684 |
| FspBI | CTAG | 5 cut(s) | 69, 495, 557, 630, 836 |
| GlaI | GCGC | 1 cut(s) | 586 |
| GluI | GCNGC | 4 cut(s) | 17, 456, 465, 684 |
| GsuI | CTGGAG | 1 cut(s) | 801 |
| HaeII | RGCGCY | 1 cut(s) | 588 |
| HaeIII | GGCC | 1 cut(s) | 429 |
| HhaI | GCGC | 1 cut(s) | 587 |
| Hin1I | GRCGYC | 1 cut(s) | 585 |
| Hin1II | CATG | 6 cut(s) | 385, 683, 799, 831, 918, 949 |
| Hin6I | GCGC | 1 cut(s) | 585 |
| HinP1I | GCGC | 1 cut(s) | 585 |
| HincII | GTYRAC | 1 cut(s) | 532 |
| HindII | GTYRAC | 1 cut(s) | 532 |
| HinfI | GANTC | 3 cut(s) | 215, 309, 813 |
| HphI | GGTGA | 1 cut(s) | 944 |
| Hpy166II | GTNNAC | 4 cut(s) | 235, 532, 652, 833 |
| Hpy188I | TCNGA | 2 cut(s) | 517, 625 |
| Hpy188III | TCNNGA | 4 cut(s) | 43, 193, 300, 796 |
| Hpy8I | GTNNAC | 4 cut(s) | 235, 532, 652, 833 |
| HpyAV | CCTTC | 4 cut(s) | 467, 743, 932, 971 |
| HpyCH4III | ACNGT | 3 cut(s) | 367, 656, 872 |
| HpyCH4IV | ACGT | 2 cut(s) | 145, 360 |
| HpyCH4V | TGCA | 9 cut(s) | 101, 269, 455, 663, 683, 716, 771, 955, 971 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 455, 461, 464, 680 |
| HpySE526I | ACGT | 2 cut(s) | 145, 360 |
| Hsp92I | GRCGYC | 1 cut(s) | 585 |
| Hsp92II | CATG | 6 cut(s) | 385, 683, 799, 831, 918, 949 |
| HspAI | GCGC | 1 cut(s) | 585 |
| KasI | GGCGCC | 1 cut(s) | 584 |
| Kzo9I | GATC | 5 cut(s) | 45, 824, 844, 861, 1004 |
| LmnI | GCTCC | 2 cut(s) | 401, 626 |
| LpnPI | CCDG | 7 cut(s) | 591, 630, 681, 726, 765, 771, 807 |
| Lsp1109I | GCAGC | 4 cut(s) | 28, 467, 476, 695 |
| LweI | GCATC | 5 cut(s) | 88, 110, 650, 766, 942 |
| MaeI | CTAG | 5 cut(s) | 69, 495, 557, 630, 836 |
| MaeII | ACGT | 2 cut(s) | 145, 360 |
| MaeIII | GTNAC | 8 cut(s) | 361, 406, 632, 698, 853, 866, 878, 900 |
| MalI | GATC | 5 cut(s) | 47, 826, 846, 863, 1006 |
| MboI | GATC | 5 cut(s) | 45, 824, 844, 861, 1004 |
| MboII | GAAGA | 4 cut(s) | 208, 296, 716, 947 |
| MflI | RGATCY | 1 cut(s) | 1004 |
| MluCI | AATT | 4 cut(s) | 248, 263, 477, 500 |
| Mly113I | GGCGCC | 1 cut(s) | 585 |
| MlyI | GAGTC | 1 cut(s) | 822 |
| MnlI | CCTC | 7 cut(s) | 101, 229, 307, 517, 619, 718, 942 |
| Mph1103I | ATGCAT | 1 cut(s) | 103 |
| MroXI | GAANNNNTTC | 1 cut(s) | 732 |
| MseI | TTAA | 6 cut(s) | 12, 162, 251, 258, 503, 738 |
| MslI | CAYNNNNRTG | 2 cut(s) | 647, 950 |
| MspA1I | CMGCKG | 1 cut(s) | 19 |
| MspCI | CTTAAG | 1 cut(s) | 737 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 455, 461, 464, 680 |
| NarI | GGCGCC | 1 cut(s) | 585 |
| NcoI | CCATGG | 1 cut(s) | 381 |
| NdeII | GATC | 5 cut(s) | 45, 824, 844, 861, 1004 |
| NlaIII | CATG | 6 cut(s) | 385, 683, 799, 831, 918, 949 |
| NlaIV | GGNNCC | 1 cut(s) | 586 |
| NmuCI | GTSAC | 2 cut(s) | 361, 878 |
| NsiI | ATGCAT | 1 cut(s) | 103 |
| OliI | CACNNNNGTG | 1 cut(s) | 647 |
| PagI | TCATGA | 1 cut(s) | 795 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 117, 849 |
| PdmI | GAANNNNTTC | 1 cut(s) | 732 |
| PfeI | GAWTC | 2 cut(s) | 215, 309 |
| PflFI | GACNNNGTC | 1 cut(s) | 64 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 387 |
| PkrI | GCNGC | 4 cut(s) | 18, 457, 466, 685 |
| Ple19I | CGATCG | 1 cut(s) | 864 |
| PleI | GAGTC | 1 cut(s) | 821 |
| PluTI | GGCGCC | 1 cut(s) | 588 |
| PpsI | GAGTC | 1 cut(s) | 821 |
| PshBI | ATTAAT | 1 cut(s) | 503 |
| PspN4I | GGNNCC | 1 cut(s) | 586 |
| PsuI | RGATCY | 1 cut(s) | 1004 |
| PsyI | GACNNNGTC | 1 cut(s) | 64 |
| PvuI | CGATCG | 1 cut(s) | 864 |
| PvuII | CAGCTG | 1 cut(s) | 19 |
| RsaI | GTAC | 3 cut(s) | 110, 372, 944 |
| RsaNI | GTAC | 3 cut(s) | 109, 371, 943 |
| RseI | CAYNNNNRTG | 2 cut(s) | 647, 950 |
| SaqAI | TTAA | 6 cut(s) | 12, 162, 251, 258, 503, 738 |
| SatI | GCNGC | 4 cut(s) | 17, 456, 465, 684 |
| Sau3AI | GATC | 5 cut(s) | 45, 824, 844, 861, 1004 |
| SchI | GAGTC | 1 cut(s) | 822 |
| SfaNI | GCATC | 5 cut(s) | 88, 110, 650, 766, 942 |
| SfoI | GGCGCC | 1 cut(s) | 586 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 647, 950 |
| SmlI | CTYRAG | 2 cut(s) | 298, 737 |
| SmoI | CTYRAG | 2 cut(s) | 298, 737 |
| SpeI | ACTAGT | 1 cut(s) | 494 |
| Sse9I | AATT | 4 cut(s) | 248, 263, 477, 500 |
| SspDI | GGCGCC | 1 cut(s) | 584 |
| SspMI | CTAG | 5 cut(s) | 69, 495, 557, 630, 836 |
| StyI | CCWWGG | 4 cut(s) | 381, 424, 556, 610 |
| TaaI | ACNGT | 3 cut(s) | 367, 656, 872 |
| TaiI | ACGT | 2 cut(s) | 148, 363 |
| TaqI | TCGA | 1 cut(s) | 843 |
| TasI | AATT | 4 cut(s) | 248, 263, 477, 500 |
| TfiI | GAWTC | 2 cut(s) | 215, 309 |
| Tru1I | TTAA | 6 cut(s) | 12, 162, 251, 258, 503, 738 |
| Tru9I | TTAA | 6 cut(s) | 12, 162, 251, 258, 503, 738 |
| TscAI | CASTG | 2 cut(s) | 465, 883 |
| TseFI | GTSAC | 2 cut(s) | 361, 878 |
| TseI | GCWGC | 4 cut(s) | 16, 455, 464, 683 |
| Tsp45I | GTSAC | 2 cut(s) | 361, 878 |
| TspDTI | ATGAA | 6 cut(s) | 17, 125, 297, 586, 784, 812 |
| TspGWI | ACGGA | 1 cut(s) | 938 |
| TspRI | CASTG | 2 cut(s) | 465, 883 |
| Tth111I | GACNNNGTC | 1 cut(s) | 64 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 387 |
| Vha464I | CTTAAG | 1 cut(s) | 737 |
| VspI | ATTAAT | 1 cut(s) | 503 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 737 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 431 |
| XmaJI | CCTAGG | 1 cut(s) | 556 |
| XmnI | GAANNNNTTC | 1 cut(s) | 732 |
| XspI | CTAG | 5 cut(s) | 69, 495, 557, 630, 836 |
| Zsp2I | ATGCAT | 1 cut(s) | 103 |