Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 153 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 533 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 638 |
| AciI | CCGC | 2 cut(s) | 178, 858 |
| AclWI | GGATC | 3 cut(s) | 40, 780, 793 |
| AcsI | RAATTY | 1 cut(s) | 287 |
| AfaI | GTAC | 4 cut(s) | 119, 616, 652, 896 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 55, 638 |
| AflII | CTTAAG | 1 cut(s) | 737 |
| AflIII | ACRYGT | 1 cut(s) | 145 |
| AgsI | TTSAA | 4 cut(s) | 184, 193, 313, 346 |
| AjiI | CACGTC | 1 cut(s) | 146 |
| AluBI | AGCT | 5 cut(s) | 371, 398, 520, 623, 1004 |
| AluI | AGCT | 5 cut(s) | 371, 398, 520, 623, 1004 |
| Alw21I | GWGCWC | 2 cut(s) | 329, 733 |
| Alw26I | GTCTC | 1 cut(s) | 986 |
| Alw44I | GTGCAC | 1 cut(s) | 729 |
| AlwI | GGATC | 3 cut(s) | 40, 780, 793 |
| AoxI | GGCC | 5 cut(s) | 39, 75, 421, 697, 741 |
| ApaLI | GTGCAC | 1 cut(s) | 729 |
| ApeKI | GCWGC | 2 cut(s) | 458, 683 |
| ApoI | RAATTY | 1 cut(s) | 287 |
| AspS9I | GGNCC | 2 cut(s) | 175, 415 |
| AsuHPI | GGTGA | 4 cut(s) | 554, 916, 932, 944 |
| AsuNHI | GCTAGC | 2 cut(s) | 359, 875 |
| AvaII | GGWCC | 2 cut(s) | 175, 415 |
| BaeGI | GKGCMC | 1 cut(s) | 733 |
| BamHI | GGATCC | 1 cut(s) | 785 |
| BbsI | GAAGAC | 1 cut(s) | 60 |
| Bbv12I | GWGCWC | 2 cut(s) | 329, 733 |
| BbvI | GCAGC | 2 cut(s) | 470, 695 |
| BccI | CCATC | 5 cut(s) | 124, 416, 565, 641, 878 |
| BceAI | ACGGC | 2 cut(s) | 437, 728 |
| BcoDI | GTCTC | 1 cut(s) | 986 |
| BfaI | CTAG | 5 cut(s) | 360, 780, 837, 842, 876 |
| BfrI | CTTAAG | 1 cut(s) | 737 |
| BisI | GCNGC | 2 cut(s) | 459, 684 |
| BlsI | GCNGC | 2 cut(s) | 460, 685 |
| BmcAI | AGTACT | 1 cut(s) | 896 |
| Bme18I | GGWCC | 2 cut(s) | 175, 415 |
| BmgBI | CACGTC | 1 cut(s) | 146 |
| BmgT120I | GGNCC | 2 cut(s) | 175, 415 |
| BmiI | GGNNCC | 2 cut(s) | 787, 826 |
| BmsI | GCATC | 5 cut(s) | 88, 110, 568, 766, 942 |
| BmtI | GCTAGC | 2 cut(s) | 363, 879 |
| BpiI | GAAGAC | 1 cut(s) | 60 |
| BpmI | CTGGAG | 1 cut(s) | 403 |
| BpuEI | CTTGAG | 1 cut(s) | 790 |
| BsaI | GGTCTC | 1 cut(s) | 986 |
| BsaJI | CCNNGG | 5 cut(s) | 418, 453, 462, 847, 996 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 55, 638 |
| Bse1I | ACTGG | 5 cut(s) | 386, 493, 643, 796, 902 |
| Bse3DI | GCAATG | 4 cut(s) | 131, 664, 886, 990 |
| BseDI | CCNNGG | 5 cut(s) | 418, 453, 462, 847, 996 |
| BseGI | GGATG | 3 cut(s) | 135, 437, 957 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 55, 638 |
| BseMI | GCAATG | 4 cut(s) | 131, 664, 886, 990 |
| BseMII | CTCAG | 4 cut(s) | 63, 462, 501, 625 |
| BseNI | ACTGG | 5 cut(s) | 386, 493, 643, 796, 902 |
| BseSI | GKGCMC | 1 cut(s) | 733 |
| BseXI | GCAGC | 2 cut(s) | 470, 695 |
| BseYI | CCCAGC | 1 cut(s) | 55 |
| BsgI | GTGCAG | 1 cut(s) | 954 |
| BshFI | GGCC | 5 cut(s) | 41, 77, 423, 699, 743 |
| BsiHKAI | GWGCWC | 2 cut(s) | 329, 733 |
| BslFI | GGGAC | 1 cut(s) | 901 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 55, 638 |
| BsmAI | GTCTC | 1 cut(s) | 986 |
| BsmFI | GGGAC | 1 cut(s) | 901 |
| BsnI | GGCC | 5 cut(s) | 41, 77, 423, 699, 743 |
| Bso31I | GGTCTC | 1 cut(s) | 986 |
| Bsp1286I | GDGCHC | 2 cut(s) | 329, 733 |
| Bsp143I | GATC | 3 cut(s) | 45, 547, 785 |
| Bsp19I | CCATGG | 3 cut(s) | 462, 847, 996 |
| BspACI | CCGC | 2 cut(s) | 178, 858 |
| BspANI | GGCC | 5 cut(s) | 41, 77, 423, 699, 743 |
| BspCNI | CTCAG | 4 cut(s) | 64, 461, 502, 624 |
| BspLI | GGNNCC | 2 cut(s) | 787, 826 |
| BspOI | GCTAGC | 2 cut(s) | 363, 879 |
| BspPI | GGATC | 3 cut(s) | 40, 780, 793 |
| BspTI | CTTAAG | 1 cut(s) | 737 |
| BspTNI | GGTCTC | 1 cut(s) | 986 |
| BsrDI | GCAATG | 4 cut(s) | 131, 664, 886, 990 |
| BsrI | ACTGG | 5 cut(s) | 386, 493, 643, 796, 902 |
| BssECI | CCNNGG | 5 cut(s) | 418, 453, 462, 847, 996 |
| BssMI | GATC | 3 cut(s) | 45, 547, 785 |
| BssT1I | CCWWGG | 4 cut(s) | 418, 462, 847, 996 |
| Bst4CI | ACNGT | 2 cut(s) | 29, 530 |
| BstAFI | CTTAAG | 1 cut(s) | 737 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 680 |
| BstC8I | GCNNGC | 5 cut(s) | 361, 365, 369, 714, 877 |
| BstDEI | CTNAG | 5 cut(s) | 72, 448, 510, 611, 891 |
| BstDSI | CCRYGG | 4 cut(s) | 453, 462, 847, 996 |
| BstF5I | GGATG | 3 cut(s) | 135, 437, 957 |
| BstKTI | GATC | 3 cut(s) | 48, 550, 788 |
| BstMAI | GTCTC | 1 cut(s) | 986 |
| BstMBI | GATC | 3 cut(s) | 45, 547, 785 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 449, 458, 680, 776, 1001 |
| BstNSI | RCATGY | 1 cut(s) | 657 |
| BstSLI | GKGCMC | 1 cut(s) | 733 |
| BstV1I | GCAGC | 2 cut(s) | 470, 695 |
| BstV2I | GAAGAC | 1 cut(s) | 60 |
| BstX2I | RGATCY | 1 cut(s) | 785 |
| BstYI | RGATCY | 1 cut(s) | 785 |
| BsuRI | GGCC | 5 cut(s) | 41, 77, 423, 699, 743 |
| BtgI | CCRYGG | 4 cut(s) | 453, 462, 847, 996 |
| BtrI | CACGTC | 1 cut(s) | 146 |
| BtsCI | GGATG | 3 cut(s) | 135, 437, 957 |
| BtsI | GCAGTG | 1 cut(s) | 1014 |
| BtsIMutI | CAGTG | 2 cut(s) | 636, 1014 |
| Cac8I | GCNNGC | 5 cut(s) | 361, 365, 369, 714, 877 |
| Cfr13I | GGNCC | 2 cut(s) | 175, 415 |
| Csp6I | GTAC | 4 cut(s) | 118, 615, 651, 895 |
| CviQI | GTAC | 4 cut(s) | 118, 615, 651, 895 |
| DdeI | CTNAG | 5 cut(s) | 72, 448, 510, 611, 891 |
| DpnI | GATC | 3 cut(s) | 47, 549, 787 |
| DpnII | GATC | 3 cut(s) | 45, 547, 785 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 533 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 533 |
| Eco130I | CCWWGG | 4 cut(s) | 418, 462, 847, 996 |
| Eco31I | GGTCTC | 1 cut(s) | 986 |
| Eco47I | GGWCC | 2 cut(s) | 175, 415 |
| EcoRI | GAATTC | 1 cut(s) | 287 |
| EcoT14I | CCWWGG | 4 cut(s) | 418, 462, 847, 996 |
| EcoT22I | ATGCAT | 1 cut(s) | 103 |
| ErhI | CCWWGG | 4 cut(s) | 418, 462, 847, 996 |
| FaqI | GGGAC | 1 cut(s) | 901 |
| Fnu4HI | GCNGC | 2 cut(s) | 459, 684 |
| FokI | GGATG | 3 cut(s) | 142, 444, 964 |
| Fsp4HI | GCNGC | 2 cut(s) | 459, 684 |
| FspBI | CTAG | 5 cut(s) | 360, 780, 837, 842, 876 |
| GluI | GCNGC | 2 cut(s) | 459, 684 |
| GsaI | CCCAGC | 1 cut(s) | 59 |
| GsuI | CTGGAG | 1 cut(s) | 403 |
| HaeIII | GGCC | 5 cut(s) | 41, 77, 423, 699, 743 |
| HincII | GTYRAC | 1 cut(s) | 526 |
| HindII | GTYRAC | 1 cut(s) | 526 |
| HindIII | AAGCTT | 1 cut(s) | 1002 |
| HinfI | GANTC | 3 cut(s) | 215, 259, 609 |
| HphI | GGTGA | 4 cut(s) | 554, 916, 932, 944 |
| Hpy166II | GTNNAC | 4 cut(s) | 9, 526, 731, 923 |
| Hpy188I | TCNGA | 3 cut(s) | 73, 511, 790 |
| Hpy8I | GTNNAC | 4 cut(s) | 9, 526, 731, 923 |
| Hpy99I | CGWCG | 1 cut(s) | 749 |
| HpyAV | CCTTC | 4 cut(s) | 461, 661, 932, 971 |
| HpyCH4III | ACNGT | 2 cut(s) | 29, 530 |
| HpyCH4IV | ACGT | 1 cut(s) | 145 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 449, 458, 680, 776, 1001 |
| HpyF3I | CTNAG | 5 cut(s) | 72, 448, 510, 611, 891 |
| HpySE526I | ACGT | 1 cut(s) | 145 |
| Kzo9I | GATC | 3 cut(s) | 45, 547, 785 |
| LmnI | GCTCC | 2 cut(s) | 332, 395 |
| Lsp1109I | GCAGC | 2 cut(s) | 470, 695 |
| LweI | GCATC | 5 cut(s) | 88, 110, 568, 766, 942 |
| MaeI | CTAG | 5 cut(s) | 360, 780, 837, 842, 876 |
| MaeII | ACGT | 1 cut(s) | 145 |
| MaeIII | GTNAC | 2 cut(s) | 400, 853 |
| MalI | GATC | 3 cut(s) | 47, 549, 787 |
| MboI | GATC | 3 cut(s) | 45, 547, 785 |
| MboII | GAAGA | 7 cut(s) | 60, 210, 296, 557, 716, 919, 947 |
| MflI | RGATCY | 1 cut(s) | 785 |
| MhlI | GDGCHC | 2 cut(s) | 329, 733 |
| MluCI | AATT | 6 cut(s) | 248, 263, 287, 471, 494, 625 |
| MlyI | GAGTC | 1 cut(s) | 603 |
| MnlI | CCTC | 7 cut(s) | 59, 67, 108, 166, 378, 553, 697 |
| Mph1103I | ATGCAT | 1 cut(s) | 103 |
| MseI | TTAA | 1 cut(s) | 738 |
| MslI | CAYNNNNRTG | 3 cut(s) | 705, 950, 995 |
| MspA1I | CMGCKG | 1 cut(s) | 860 |
| MspCI | CTTAAG | 1 cut(s) | 737 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 449, 458, 680, 776, 1001 |
| NcoI | CCATGG | 3 cut(s) | 462, 847, 996 |
| NdeII | GATC | 3 cut(s) | 45, 547, 785 |
| NheI | GCTAGC | 2 cut(s) | 359, 875 |
| NlaIV | GGNNCC | 2 cut(s) | 787, 826 |
| NsiI | ATGCAT | 1 cut(s) | 103 |
| NspI | RCATGY | 1 cut(s) | 657 |
| PfeI | GAWTC | 2 cut(s) | 215, 259 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 638 |
| PkrI | GCNGC | 2 cut(s) | 460, 685 |
| PleI | GAGTC | 1 cut(s) | 603 |
| PpsI | GAGTC | 1 cut(s) | 603 |
| PsiI | TTATAA | 1 cut(s) | 153 |
| PspFI | CCCAGC | 1 cut(s) | 55 |
| PspN4I | GGNNCC | 2 cut(s) | 787, 826 |
| PspPI | GGNCC | 2 cut(s) | 175, 415 |
| PsuI | RGATCY | 1 cut(s) | 785 |
| RsaI | GTAC | 4 cut(s) | 119, 616, 652, 896 |
| RsaNI | GTAC | 4 cut(s) | 118, 615, 651, 895 |
| RseI | CAYNNNNRTG | 3 cut(s) | 705, 950, 995 |
| SaqAI | TTAA | 1 cut(s) | 738 |
| SatI | GCNGC | 2 cut(s) | 459, 684 |
| Sau3AI | GATC | 3 cut(s) | 45, 547, 785 |
| Sau96I | GGNCC | 2 cut(s) | 175, 415 |
| ScaI | AGTACT | 1 cut(s) | 896 |
| SchI | GAGTC | 1 cut(s) | 603 |
| SduI | GDGCHC | 2 cut(s) | 329, 733 |
| SfaNI | GCATC | 5 cut(s) | 88, 110, 568, 766, 942 |
| SinI | GGWCC | 2 cut(s) | 175, 415 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 705, 950, 995 |
| SmlI | CTYRAG | 2 cut(s) | 737, 805 |
| SmoI | CTYRAG | 2 cut(s) | 737, 805 |
| Sse9I | AATT | 6 cut(s) | 248, 263, 287, 471, 494, 625 |
| SsiI | CCGC | 2 cut(s) | 178, 858 |
| SspI | AATATT | 1 cut(s) | 189 |
| SspMI | CTAG | 5 cut(s) | 360, 780, 837, 842, 876 |
| StyI | CCWWGG | 4 cut(s) | 418, 462, 847, 996 |
| TaaI | ACNGT | 2 cut(s) | 29, 530 |
| TaiI | ACGT | 1 cut(s) | 148 |
| TaqII | GACCGA | 1 cut(s) | 179 |
| TasI | AATT | 6 cut(s) | 248, 263, 287, 471, 494, 625 |
| TatI | WGTACW | 1 cut(s) | 894 |
| TfiI | GAWTC | 2 cut(s) | 215, 259 |
| Tru1I | TTAA | 1 cut(s) | 738 |
| Tru9I | TTAA | 1 cut(s) | 738 |
| TscAI | CASTG | 2 cut(s) | 643, 1021 |
| TseI | GCWGC | 2 cut(s) | 458, 683 |
| TspDTI | ATGAA | 3 cut(s) | 297, 366, 580 |
| TspRI | CASTG | 2 cut(s) | 643, 1021 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 638 |
| Vha464I | CTTAAG | 1 cut(s) | 737 |
| VneI | GTGCAC | 1 cut(s) | 729 |
| VpaK11BI | GGWCC | 2 cut(s) | 175, 415 |
| XapI | RAATTY | 1 cut(s) | 287 |
| XceI | RCATGY | 1 cut(s) | 657 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 425 |
| XspI | CTAG | 5 cut(s) | 360, 780, 837, 842, 876 |
| ZrmI | AGTACT | 1 cut(s) | 896 |
| Zsp2I | ATGCAT | 1 cut(s) | 103 |