Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 24 |
| AccII | CGCG | 1 cut(s) | 615 |
| AccIII | TCCGGA | 1 cut(s) | 74 |
| AciI | CCGC | 9 cut(s) | 438, 468, 546, 573, 615, 750, 867, 980, 983 |
| AcoI | YGGCCR | 1 cut(s) | 465 |
| AcsI | RAATTY | 1 cut(s) | 358 |
| AfaI | GTAC | 3 cut(s) | 113, 661, 953 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 58, 421, 647 |
| AflIII | ACRYGT | 1 cut(s) | 662 |
| AgsI | TTSAA | 7 cut(s) | 187, 196, 295, 316, 349, 713, 816 |
| AluBI | AGCT | 7 cut(s) | 45, 490, 529, 632, 758, 947, 1028 |
| AluI | AGCT | 7 cut(s) | 45, 490, 529, 632, 758, 947, 1028 |
| Alw21I | GWGCWC | 2 cut(s) | 332, 531 |
| Alw26I | GTCTC | 1 cut(s) | 170 |
| Aor13HI | TCCGGA | 1 cut(s) | 74 |
| AoxI | GGCC | 3 cut(s) | 430, 465, 738 |
| ApeKI | GCWGC | 4 cut(s) | 19, 692, 977, 1010 |
| ApoI | RAATTY | 1 cut(s) | 358 |
| AspS9I | GGNCC | 3 cut(s) | 424, 739, 834 |
| AsuC2I | CCSGG | 1 cut(s) | 832 |
| AsuHPI | GGTGA | 3 cut(s) | 563, 752, 953 |
| AvaII | GGWCC | 2 cut(s) | 424, 834 |
| BanII | GRGCYC | 1 cut(s) | 531 |
| BauI | CACGAG | 1 cut(s) | 323 |
| BbsI | GAAGAC | 2 cut(s) | 344, 561 |
| Bbv12I | GWGCWC | 2 cut(s) | 332, 531 |
| BbvI | GCAGC | 4 cut(s) | 6, 704, 964, 997 |
| BccI | CCATC | 4 cut(s) | 425, 574, 650, 887 |
| BceAI | ACGGC | 2 cut(s) | 7, 672 |
| BcnI | CCSGG | 1 cut(s) | 832 |
| BcoDI | GTCTC | 1 cut(s) | 170 |
| BfaI | CTAG | 2 cut(s) | 789, 911 |
| BfuAI | ACCTGC | 1 cut(s) | 24 |
| BisI | GCNGC | 7 cut(s) | 20, 468, 574, 693, 978, 981, 1011 |
| BlsI | GCNGC | 7 cut(s) | 21, 469, 575, 694, 979, 982, 1012 |
| Bme1390I | CCNGG | 1 cut(s) | 832 |
| Bme18I | GGWCC | 2 cut(s) | 424, 834 |
| BmgT120I | GGNCC | 3 cut(s) | 424, 739, 834 |
| BmiI | GGNNCC | 2 cut(s) | 79, 835 |
| BmrFI | CCNGG | 1 cut(s) | 832 |
| BmrI | ACTGGG | 1 cut(s) | 507 |
| BmsI | GCATC | 6 cut(s) | 91, 223, 377, 431, 577, 775 |
| BmuI | ACTGGG | 1 cut(s) | 507 |
| BpiI | GAAGAC | 2 cut(s) | 344, 561 |
| BpuEI | CTTGAG | 2 cut(s) | 61, 286 |
| BpuMI | CCSGG | 1 cut(s) | 832 |
| BsaJI | CCNNGG | 5 cut(s) | 23, 427, 471, 559, 856 |
| BsaWI | WCCGGW | 1 cut(s) | 74 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 314, 344 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 58, 421, 647 |
| Bse1I | ACTGG | 4 cut(s) | 502, 595, 709, 805 |
| Bse3DI | GCAATG | 2 cut(s) | 134, 999 |
| BseAI | TCCGGA | 1 cut(s) | 74 |
| BseDI | CCNNGG | 5 cut(s) | 23, 427, 471, 559, 856 |
| BseGI | GGATG | 8 cut(s) | 71, 114, 238, 446, 569, 589, 600, 898 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 58, 421, 647 |
| BseMI | GCAATG | 2 cut(s) | 134, 999 |
| BseMII | CTCAG | 1 cut(s) | 510 |
| BseNI | ACTGG | 4 cut(s) | 502, 595, 709, 805 |
| BseXI | GCAGC | 4 cut(s) | 6, 704, 964, 997 |
| BseYI | CCCAGC | 2 cut(s) | 58, 947 |
| BsgI | GTGCAG | 2 cut(s) | 711, 951 |
| Bsh1236I | CGCG | 1 cut(s) | 615 |
| BshFI | GGCC | 3 cut(s) | 432, 467, 740 |
| BsiHKAI | GWGCWC | 2 cut(s) | 332, 531 |
| BsiSI | CCGG | 2 cut(s) | 75, 831 |
| BslFI | GGGAC | 4 cut(s) | 333, 704, 847, 910 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 58, 421, 647 |
| BsmAI | GTCTC | 1 cut(s) | 170 |
| BsmFI | GGGAC | 4 cut(s) | 333, 704, 847, 910 |
| BsmI | GAATGC | 1 cut(s) | 966 |
| BsnI | GGCC | 3 cut(s) | 432, 467, 740 |
| Bsp1286I | GDGCHC | 2 cut(s) | 332, 531 |
| Bsp13I | TCCGGA | 1 cut(s) | 74 |
| Bsp143I | GATC | 1 cut(s) | 507 |
| Bsp19I | CCATGG | 1 cut(s) | 856 |
| BspACI | CCGC | 9 cut(s) | 438, 468, 546, 573, 615, 750, 867, 980, 983 |
| BspANI | GGCC | 3 cut(s) | 432, 467, 740 |
| BspCNI | CTCAG | 1 cut(s) | 511 |
| BspEI | TCCGGA | 1 cut(s) | 74 |
| BspFNI | CGCG | 1 cut(s) | 615 |
| BspLI | GGNNCC | 2 cut(s) | 79, 835 |
| BspMI | ACCTGC | 1 cut(s) | 24 |
| BsrDI | GCAATG | 2 cut(s) | 134, 999 |
| BsrI | ACTGG | 4 cut(s) | 502, 595, 709, 805 |
| BssECI | CCNNGG | 5 cut(s) | 23, 427, 471, 559, 856 |
| BssMI | GATC | 1 cut(s) | 507 |
| BssSI | CACGAG | 1 cut(s) | 323 |
| BssT1I | CCWWGG | 3 cut(s) | 427, 559, 856 |
| Bst2BI | CACGAG | 1 cut(s) | 323 |
| Bst4CI | ACNGT | 2 cut(s) | 650, 731 |
| Bst6I | CTCTTC | 1 cut(s) | 282 |
| BstC8I | GCNNGC | 1 cut(s) | 685 |
| BstDEI | CTNAG | 3 cut(s) | 519, 900, 1029 |
| BstDSI | CCRYGG | 3 cut(s) | 23, 471, 856 |
| BstF5I | GGATG | 8 cut(s) | 71, 114, 238, 446, 569, 589, 600, 898 |
| BstFNI | CGCG | 1 cut(s) | 615 |
| BstKTI | GATC | 1 cut(s) | 510 |
| BstMAI | GTCTC | 1 cut(s) | 170 |
| BstMBI | GATC | 1 cut(s) | 507 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 458, 467, 621, 689, 785, 1010 |
| BstNSI | RCATGY | 1 cut(s) | 666 |
| BstSCI | CCNGG | 1 cut(s) | 830 |
| BstUI | CGCG | 1 cut(s) | 615 |
| BstV1I | GCAGC | 4 cut(s) | 6, 704, 964, 997 |
| BstV2I | GAAGAC | 2 cut(s) | 344, 561 |
| BsuRI | GGCC | 3 cut(s) | 432, 467, 740 |
| BtgI | CCRYGG | 3 cut(s) | 23, 471, 856 |
| BtsCI | GGATG | 8 cut(s) | 71, 114, 238, 446, 569, 589, 600, 898 |
| BtsI | GCAGTG | 1 cut(s) | 468 |
| BtsIMutI | CAGTG | 2 cut(s) | 468, 736 |
| BveI | ACCTGC | 1 cut(s) | 24 |
| Cac8I | GCNNGC | 1 cut(s) | 685 |
| Cfr13I | GGNCC | 3 cut(s) | 424, 739, 834 |
| Csp6I | GTAC | 3 cut(s) | 112, 660, 952 |
| CviAII | CATG | 5 cut(s) | 55, 105, 663, 857, 924 |
| CviQI | GTAC | 3 cut(s) | 112, 660, 952 |
| DdeI | CTNAG | 3 cut(s) | 519, 900, 1029 |
| DpnI | GATC | 1 cut(s) | 509 |
| DpnII | GATC | 1 cut(s) | 507 |
| EaeI | YGGCCR | 1 cut(s) | 465 |
| Eam1104I | CTCTTC | 1 cut(s) | 282 |
| EarI | CTCTTC | 1 cut(s) | 282 |
| EciI | GGCGGA | 1 cut(s) | 998 |
| Ecl136II | GAGCTC | 1 cut(s) | 529 |
| Eco130I | CCWWGG | 3 cut(s) | 427, 559, 856 |
| Eco24I | GRGCYC | 1 cut(s) | 531 |
| Eco47I | GGWCC | 2 cut(s) | 424, 834 |
| Eco53kI | GAGCTC | 1 cut(s) | 529 |
| EcoICRI | GAGCTC | 1 cut(s) | 529 |
| EcoO109I | RGGNCCY | 2 cut(s) | 739, 834 |
| EcoT14I | CCWWGG | 3 cut(s) | 427, 559, 856 |
| EcoT22I | ATGCAT | 1 cut(s) | 106 |
| EcoT38I | GRGCYC | 1 cut(s) | 531 |
| ErhI | CCWWGG | 3 cut(s) | 427, 559, 856 |
| FaeI | CATG | 5 cut(s) | 58, 108, 666, 860, 927 |
| FaqI | GGGAC | 4 cut(s) | 333, 704, 847, 910 |
| FatI | CATG | 5 cut(s) | 54, 104, 662, 856, 923 |
| FauI | CCCGC | 1 cut(s) | 608 |
| Fnu4HI | GCNGC | 7 cut(s) | 20, 468, 574, 693, 978, 981, 1011 |
| FokI | GGATG | 8 cut(s) | 58, 121, 245, 453, 576, 587, 596, 905 |
| FriOI | GRGCYC | 1 cut(s) | 531 |
| Fsp4HI | GCNGC | 7 cut(s) | 20, 468, 574, 693, 978, 981, 1011 |
| FspBI | CTAG | 2 cut(s) | 789, 911 |
| GluI | GCNGC | 7 cut(s) | 20, 468, 574, 693, 978, 981, 1011 |
| GsaI | CCCAGC | 2 cut(s) | 62, 951 |
| HaeIII | GGCC | 3 cut(s) | 432, 467, 740 |
| HapII | CCGG | 2 cut(s) | 75, 831 |
| Hin1II | CATG | 5 cut(s) | 58, 108, 666, 860, 927 |
| HincII | GTYRAC | 1 cut(s) | 135 |
| HindII | GTYRAC | 1 cut(s) | 135 |
| HinfI | GANTC | 4 cut(s) | 136, 178, 218, 312 |
| HpaII | CCGG | 2 cut(s) | 75, 831 |
| HphI | GGTGA | 3 cut(s) | 563, 752, 953 |
| Hpy166II | GTNNAC | 1 cut(s) | 135 |
| Hpy188I | TCNGA | 2 cut(s) | 520, 799 |
| Hpy188III | TCNNGA | 3 cut(s) | 75, 187, 303 |
| Hpy8I | GTNNAC | 1 cut(s) | 135 |
| Hpy99I | CGWCG | 2 cut(s) | 164, 650 |
| HpyAV | CCTTC | 6 cut(s) | 166, 470, 601, 670, 752, 980 |
| HpyCH4III | ACNGT | 2 cut(s) | 650, 731 |
| HpyCH4IV | ACGT | 1 cut(s) | 162 |
| HpyCH4V | TGCA | 7 cut(s) | 104, 272, 368, 692, 725, 932, 964 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 458, 467, 621, 689, 785, 1010 |
| HpyF3I | CTNAG | 3 cut(s) | 519, 900, 1029 |
| HpySE526I | ACGT | 1 cut(s) | 162 |
| Hsp92II | CATG | 5 cut(s) | 58, 108, 666, 860, 927 |
| Kpn2I | TCCGGA | 1 cut(s) | 74 |
| Kzo9I | GATC | 1 cut(s) | 507 |
| LmnI | GCTCC | 3 cut(s) | 77, 335, 404 |
| Lsp1109I | GCAGC | 4 cut(s) | 6, 704, 964, 997 |
| LweI | GCATC | 6 cut(s) | 91, 223, 377, 431, 577, 775 |
| MaeI | CTAG | 2 cut(s) | 789, 911 |
| MaeII | ACGT | 1 cut(s) | 162 |
| MaeIII | GTNAC | 4 cut(s) | 409, 539, 665, 862 |
| MalI | GATC | 1 cut(s) | 509 |
| MboI | GATC | 1 cut(s) | 507 |
| MboII | GAAGA | 6 cut(s) | 213, 299, 349, 566, 725, 928 |
| MhlI | GDGCHC | 2 cut(s) | 332, 531 |
| MluCI | AATT | 5 cut(s) | 266, 358, 480, 503, 634 |
| MlyI | GAGTC | 2 cut(s) | 130, 172 |
| MnlI | CCTC | 5 cut(s) | 62, 310, 562, 883, 1024 |
| Mph1103I | ATGCAT | 1 cut(s) | 106 |
| MroI | TCCGGA | 1 cut(s) | 74 |
| MseI | TTAA | 3 cut(s) | 12, 165, 254 |
| MslI | CAYNNNNRTG | 2 cut(s) | 959, 1004 |
| MspA1I | CMGCKG | 2 cut(s) | 548, 869 |
| MspI | CCGG | 2 cut(s) | 75, 831 |
| MspR9I | CCNGG | 1 cut(s) | 832 |
| Mva1269I | GAATGC | 1 cut(s) | 966 |
| MvnI | CGCG | 1 cut(s) | 615 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 458, 467, 621, 689, 785, 1010 |
| NciI | CCSGG | 1 cut(s) | 832 |
| NcoI | CCATGG | 1 cut(s) | 856 |
| NdeII | GATC | 1 cut(s) | 507 |
| NlaIII | CATG | 5 cut(s) | 58, 108, 666, 860, 927 |
| NlaIV | GGNNCC | 2 cut(s) | 79, 835 |
| NmuCI | GTSAC | 1 cut(s) | 539 |
| NsiI | ATGCAT | 1 cut(s) | 106 |
| NspI | RCATGY | 1 cut(s) | 666 |
| PciI | ACATGT | 1 cut(s) | 662 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 120, 145 |
| PctI | GAATGC | 1 cut(s) | 966 |
| PfeI | GAWTC | 2 cut(s) | 218, 312 |
| PkrI | GCNGC | 7 cut(s) | 21, 469, 575, 694, 979, 982, 1012 |
| PleI | GAGTC | 2 cut(s) | 130, 172 |
| PpsI | GAGTC | 2 cut(s) | 130, 172 |
| PpuMI | RGGWCCY | 1 cut(s) | 834 |
| PscI | ACATGT | 1 cut(s) | 662 |
| Psp124BI | GAGCTC | 1 cut(s) | 531 |
| Psp5II | RGGWCCY | 1 cut(s) | 834 |
| PspFI | CCCAGC | 2 cut(s) | 58, 947 |
| PspN4I | GGNNCC | 2 cut(s) | 79, 835 |
| PspPI | GGNCC | 3 cut(s) | 424, 739, 834 |
| PspPPI | RGGWCCY | 1 cut(s) | 834 |
| RsaI | GTAC | 3 cut(s) | 113, 661, 953 |
| RsaNI | GTAC | 3 cut(s) | 112, 660, 952 |
| RseI | CAYNNNNRTG | 2 cut(s) | 959, 1004 |
| SacI | GAGCTC | 1 cut(s) | 531 |
| SaqAI | TTAA | 3 cut(s) | 12, 165, 254 |
| SatI | GCNGC | 7 cut(s) | 20, 468, 574, 693, 978, 981, 1011 |
| Sau3AI | GATC | 1 cut(s) | 507 |
| Sau96I | GGNCC | 3 cut(s) | 424, 739, 834 |
| SchI | GAGTC | 2 cut(s) | 130, 172 |
| ScrFI | CCNGG | 1 cut(s) | 832 |
| SduI | GDGCHC | 2 cut(s) | 332, 531 |
| SfaNI | GCATC | 6 cut(s) | 91, 223, 377, 431, 577, 775 |
| SinI | GGWCC | 2 cut(s) | 424, 834 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 959, 1004 |
| SmlI | CTYRAG | 2 cut(s) | 40, 301 |
| SmoI | CTYRAG | 2 cut(s) | 40, 301 |
| Sse9I | AATT | 5 cut(s) | 266, 358, 480, 503, 634 |
| SsiI | CCGC | 9 cut(s) | 438, 468, 546, 573, 615, 750, 867, 980, 983 |
| SspMI | CTAG | 2 cut(s) | 789, 911 |
| SstI | GAGCTC | 1 cut(s) | 531 |
| StyD4I | CCNGG | 1 cut(s) | 830 |
| StyI | CCWWGG | 3 cut(s) | 427, 559, 856 |
| TaaI | ACNGT | 2 cut(s) | 650, 731 |
| TaiI | ACGT | 1 cut(s) | 165 |
| TasI | AATT | 5 cut(s) | 266, 358, 480, 503, 634 |
| TauI | GCSGC | 3 cut(s) | 470, 576, 983 |
| TfiI | GAWTC | 2 cut(s) | 218, 312 |
| Tru1I | TTAA | 3 cut(s) | 12, 165, 254 |
| Tru9I | TTAA | 3 cut(s) | 12, 165, 254 |
| TscAI | CASTG | 2 cut(s) | 468, 736 |
| TseFI | GTSAC | 1 cut(s) | 539 |
| TseI | GCWGC | 4 cut(s) | 19, 692, 977, 1010 |
| Tsp45I | GTSAC | 1 cut(s) | 539 |
| TspDTI | ATGAA | 3 cut(s) | 300, 369, 589 |
| TspGWI | ACGGA | 1 cut(s) | 488 |
| TspRI | CASTG | 2 cut(s) | 468, 736 |
| VpaK11BI | GGWCC | 2 cut(s) | 424, 834 |
| XapI | RAATTY | 1 cut(s) | 358 |
| XceI | RCATGY | 1 cut(s) | 666 |
| XspI | CTAG | 2 cut(s) | 789, 911 |
| Zsp2I | ATGCAT | 1 cut(s) | 106 |