Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000141

Physical Location & Seq

Genomic Coordinates Forward (+)

27990003 .. 27991152

Gene Structure 1150 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1231700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 927 bp

ATGGAGAACCTAAACCTGCAGCTATGGAAGTATATGTGCTTGGGTTTGATCCTCATGTTGGGAGCTTGGTCTTCTCAAGCCACTTCTCGCAGTCTGCAAGATGCATCAATGTATGGGAGATACGAGCAATGGTTGGCTCGTTATGGCCGTGTATATAATGACGTCGATGAGAAGGAAACTCGTTTCAAGATATTTAAGGAAAATGTGGCGTTTATAGAATCTTCTAATAAGGATGCAAACAAACCTTACAAATTAAGCGTCAATCAATTTTCAGACCTTACAAATGAAGAGTTCAAAGCCTCAGAAATGGATTCAAGGGCCATGAATGATGCTGTTGGGCTTTTTCAGCAGTTGCCGCCACCGAAGGTGTTACACAGCTTACAAACTGGTAAATTGATCTCTTTGTCTGAGCAAGAGCTCGTTGATTGTGACACCGCTGGTGAAGACCAAGGTTGTGAGGGTGGCTTAATGGACGATGCGTTTCGTTTCAGTTCATCCAACAAAAATCACGGTATTAGCACAGAAACTAATTACCCCTACAACGGTGTTGATGGTACATGTAACACCAAGAAGGAAGCCATCATTGCAGCCAAGATAACTGGCTTTGAGGATGTGCCTGCAAATAGTGAAAAGGCCCTTCTAACCGCTGTTGCTCATCAACCTGTTTCCGTTGCCATCGATGCTAGCGGTTCCGACTTCCAATTCTATTCAAGTGGTGTCTTCACCGGAACTTGTGGAACGAGTCTTGACCATGGTGTTACCGCTGTTGGATATGGCGTGAGTGAGGATGGGACTAAGTATTGGCTAGTGAAGAACTCATGGGGTGCAGAATGGGGCGAAACTGGGTACATAAGAATGCAAAGAGATGTTGCTGCACCTGAAGGACTTTGTGGGATTGCTATGTCTGCCTCTTACCCCACAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

309

Amino Acids

33.77

Weight (kDa)

4.75

Isoelectric Point (pI)

30.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	41 - 98	8.6e-19	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	127 - 307	3.8e-61	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000141)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22160
fragaria_vesca	FvH4_1g23580 FvH4_1g25982 FvH4_2g17070 FvH4_3g40500 FvH4_6g27180 FvH4_6g27320 FvH4_6g33300 FvH4_6g33310 FvH4_7g09480 FvH4_7g13000 FvH4_7g13320 FvH4_7g13600
malus_domestica	MD00G1208000.v1.1 MD09G1238200.v1.1 MD09G1238600.v1.1 MD09G1238800.v1.1 MD09G1239100.v1.1 MD16G1226900.v1.1 MD16G1242700.v1.1 MD16G1244200.v1.1 MD16G1245500.v1.1 MD16G1245800.v1.1 MD17G1231600.v1.1 MD17G1231700.v1.1 MD17G1231800.v1.1 MD17G1232200.v1.1 MD17G1232300.v1.1 MD17G1232400.v1.1
prunus_persica	Prupe.1G072300_v2.0.a1 Prupe.1G072400_v2.0.a1 Prupe.3G106300_v2.0.a1 Prupe.3G106400_v2.0.a1 Prupe.3G119400_v2.0.a1 Prupe.3G120600_v2.0.a1 Prupe.3G121900_v2.0.a1 Prupe.3G122000_v2.0.a1 Prupe.3G122100_v2.0.a1 Prupe.3G122200_v2.0.a1 Prupe.3G122700_v2.0.a1 Prupe.5G055900_v2.0.a1 Prupe.5G056500_v2.0.a1
pyrus_communis	pycom09g15770 pycom09g15780 pycom09g15790 pycom16g20640 pycom17g18390
rosa_chinensis	RchiOBHm_Chr1g0352141 RchiOBHm_Chr1g0352581 RchiOBHm_Chr1g0352601 RchiOBHm_Chr1g0352621 RchiOBHm_Chr1g0352631 RchiOBHm_Chr2g0119131 RchiOBHm_Chr2g0128691 RchiOBHm_Chr2g0128701 RchiOBHm_Chr2g0129151 RchiOBHm_Chr2g0129221 RchiOBHm_Chr2g0129401 RchiOBHm_Chr2g0129421 RchiOBHm_Chr2g0129431 RchiOBHm_Chr2g0129471 RchiOBHm_Chr2g0129481 RchiOBHm_Chr2g0129491 RchiOBHm_Chr2g0143051 RchiOBHm_Chr2g0143121 RchiOBHm_Chr2g0143171 RchiOBHm_Chr3g0457341 RchiOBHm_Chr5g0049011 RchiOBHm_Chr6g0281281 RchiOBHm_Chr6g0281311
rosa_laevigata	RLG00000012975 RLG00000012976 RLG00000012979 RLG00000018447 RLG00000019018 RLG00000019061 RLG00000019066 RLG00000019078 RLG00000019079 RLG00000019080 RLG00000019083 RLG00000019085 RLG00000020009 RLG00000020010 RLG00000025234 RLG00000028326 RLG00000028327 RLG00000028328 RLG00000028329 RLG00000028357 RLG00000031784
rosa_multiflora	Rmu_co8169188.1_g000001 Rmu_co8261007.1_g000001 Rmu_co8314361.1_g000001 Rmu_sc0000109.1_g000014 Rmu_sc0000638.1_g000020 Rmu_sc0000847.1_g000020 Rmu_sc0000847.1_g000028 Rmu_sc0000847.1_g000037 Rmu_sc0000847.1_g000046 Rmu_sc0000911.1_g000034 Rmu_sc0000999.1_g000021 Rmu_sc0001171.1_g000013 Rmu_sc0001171.1_g000018 Rmu_sc0001171.1_g000019 Rmu_sc0001653.1_g000004 Rmu_sc0002277.1_g000005 Rmu_sc0003479.1_g000001 Rmu_sc0005118.1_g000006 Rmu_sc0005118.1_g000008 Rmu_sc0005227.1_g000012 Rmu_sc0005442.1_g000010 Rmu_sc0005514.1_g000005 Rmu_sc0005996.1_g000014 Rmu_sc0008800.1_g000002 Rmu_sc0008800.1_g000010 Rmu_sc0008800.1_g000014
rosa_roxburghii	Rroxscaffold_1G00065560 Rroxscaffold_2G00102080 Rroxscaffold_2G00102090 Rroxscaffold_2G00102110 Rroxscaffold_2G00114560 Rroxscaffold_2G00114570 Rroxscaffold_2G00114610 Rroxscaffold_2G00114620 Rroxscaffold_2G00114640 Rroxscaffold_2G00114740 Rroxscaffold_2G00114790 Rroxscaffold_2G00115860 Rroxscaffold_2G00124490 Rroxscaffold_4G00302260 Rroxscaffold_4G00302530 Rroxscaffold_4G00302550 Rroxscaffold_4G00302560 Rroxscaffold_4G00302620 Rroxscaffold_6G00421980 Rroxscaffold_7G00187140 Rroxscaffold_7G00187170
rosa_rugosa	Rorug01G0225000 Rorug01G0230200 Rorug02G0220500 Rorug02G0280000 Rorug02G0285200 Rorug02G0285400 Rorug02G0286300 Rorug02G0286300 Rorug02G0286400 Rorug02G0286900 Rorug02G0377800 Rorug02G0377900 Rorug02G0378000 Rorug02G0378000 Rorug02G0378800 Rorug03G0019800 Rorug04G0452700 Rorug06G0140300 Rorug06G0140500 Rorug06G0140600
rosa_samantha	Rh1CG223600 Rh1CG227200 Rh1CG227500 Rh1CG227600 Rh2BG341200 Rh2BG345300 Rh2BG440200 Rh2DG284500 Rh2DG357900 Rh2DG362100 Rh2DG362600 Rh2DG364500 Rh2DG364600 Rh2DG364700 Rh2DG364800 Rh2DG364900 Rh2DG365000 Rh2DG365400 Rh2DG450400 Rh3AG080000 Rh3BG081900 Rh5AG322100 Rh5BG079800 Rh6AG252100 Rh6BG255600
rosa_wichuraiana	Rw1G020770 Rw1G021290 Rw2G022120 Rw2G026940 Rw2G027360 Rw2G027370 Rw2G027950 Rw2G027960 Rw2G027970 Rw2G035220 Rw3G006890 Rw6G021900 Rw6G021930 Rw6G021950 Rw6G021970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	165
Acc36I	ACCTGC	1 cut(s)	24
AciI	CCGC	5 cut(s)	356, 435, 645, 687, 762
AclWI	GGATC	1 cut(s)	43
AcoI	YGGCCR	1 cut(s)	145
AcuI	CTGAAG	1 cut(s)	900
AcyI	GRCGYC	1 cut(s)	162
AfaI	GTAC	2 cut(s)	556, 848
AfiI	CCNNNNNNNGG	2 cut(s)	58, 542
AflIII	ACRYGT	1 cut(s)	557
AgsI	TTSAA	4 cut(s)	187, 295, 315, 711
AluBI	AGCT	5 cut(s)	22, 65, 378, 418, 923
AluI	AGCT	5 cut(s)	22, 65, 378, 418, 923
Alw21I	GWGCWC	1 cut(s)	420
AlwI	GGATC	1 cut(s)	43
AoxI	GGCC	3 cut(s)	145, 318, 633
ApeKI	GCWGC	3 cut(s)	19, 587, 872
ArsI	GACNNNNNNTTYG	2 cut(s)	243, 275
AspS9I	GGNCC	2 cut(s)	318, 634
AsuHPI	GGTGA	2 cut(s)	452, 715
AsuNHI	GCTAGC	1 cut(s)	683
BanII	GRGCYC	1 cut(s)	420
BbsI	GAAGAC	3 cut(s)	63, 450, 712
Bbv12I	GWGCWC	1 cut(s)	420
BbvI	GCAGC	3 cut(s)	31, 599, 859
BccI	CCATC	4 cut(s)	545, 587, 683, 782
BceAI	ACGGC	1 cut(s)	132
BfaI	CTAG	2 cut(s)	684, 806
BfmI	CTRYAG	1 cut(s)	17
BfuAI	ACCTGC	1 cut(s)	24
BisI	GCNGC	4 cut(s)	20, 356, 588, 873
BlsI	GCNGC	4 cut(s)	21, 357, 589, 874
BmgT120I	GGNCC	2 cut(s)	318, 634
BmiI	GGNNCC	1 cut(s)	691
BmrI	ACTGGG	1 cut(s)	852
BmsI	GCATC	6 cut(s)	91, 113, 223, 319, 466, 670
BmtI	GCTAGC	1 cut(s)	687
BmuI	ACTGGG	1 cut(s)	852
BpiI	GAAGAC	3 cut(s)	63, 450, 712
BpuEI	CTTGAG	1 cut(s)	60
Bsa29I	ATCGAT	1 cut(s)	678
BsaHI	GRCGYC	1 cut(s)	162
BsaJI	CCNNGG	2 cut(s)	448, 751
BsaWI	WCCGGW	1 cut(s)	725
Bsc4I	CCNNNNNNNGG	2 cut(s)	58, 542
Bse1I	ACTGG	3 cut(s)	391, 604, 847
Bse3DI	GCAATG	2 cut(s)	134, 582
BseCI	ATCGAT	1 cut(s)	678
BseDI	CCNNGG	2 cut(s)	448, 751
BseGI	GGATG	4 cut(s)	238, 494, 616, 793
BseLI	CCNNNNNNNGG	2 cut(s)	58, 542
BseMI	GCAATG	2 cut(s)	134, 582
BseMII	CTCAG	2 cut(s)	315, 399
BseNI	ACTGG	3 cut(s)	391, 604, 847
BseXI	GCAGC	3 cut(s)	31, 599, 859
BsgI	GTGCAG	2 cut(s)	846, 858
BshFI	GGCC	3 cut(s)	147, 320, 635
BshVI	ATCGAT	1 cut(s)	678
BsiHKAI	GWGCWC	1 cut(s)	420
BsiSI	CCGG	1 cut(s)	726
BslFI	GGGAC	1 cut(s)	805
BslI	CCNNNNNNNGG	2 cut(s)	58, 542
BsmFI	GGGAC	1 cut(s)	805
BsmI	GAATGC	1 cut(s)	861
BsnI	GGCC	3 cut(s)	147, 320, 635
Bsp1286I	GDGCHC	1 cut(s)	420
Bsp143I	GATC	2 cut(s)	48, 396
Bsp19I	CCATGG	1 cut(s)	751
BspACI	CCGC	5 cut(s)	356, 435, 645, 687, 762
BspANI	GGCC	3 cut(s)	147, 320, 635
BspCNI	CTCAG	2 cut(s)	314, 400
BspDI	ATCGAT	1 cut(s)	678
BspLI	GGNNCC	1 cut(s)	691
BspMAI	CTGCAG	1 cut(s)	21
BspMI	ACCTGC	1 cut(s)	24
BspOI	GCTAGC	1 cut(s)	687
BspPI	GGATC	1 cut(s)	43
BsrDI	GCAATG	2 cut(s)	134, 582
BsrI	ACTGG	3 cut(s)	391, 604, 847
BssECI	CCNNGG	2 cut(s)	448, 751
BssMI	GATC	2 cut(s)	48, 396
BssNI	GRCGYC	1 cut(s)	162
BssT1I	CCWWGG	2 cut(s)	448, 751
Bst4CI	ACNGT	2 cut(s)	512, 545
Bst6I	CTCTTC	1 cut(s)	282
BstACI	GRCGYC	1 cut(s)	162
BstC8I	GCNNGC	2 cut(s)	618, 685
BstDEI	CTNAG	4 cut(s)	301, 408, 795, 924
BstDSI	CCRYGG	1 cut(s)	751
BstF5I	GGATG	4 cut(s)	238, 494, 616, 793
BstKTI	GATC	2 cut(s)	51, 399
BstMBI	GATC	2 cut(s)	48, 396
BstMWI	GCNNNNNNNGC	5 cut(s)	346, 355, 584, 680, 905
BstNSI	RCATGY	1 cut(s)	561
BstSFI	CTRYAG	1 cut(s)	17
BstV1I	GCAGC	3 cut(s)	31, 599, 859
BstV2I	GAAGAC	3 cut(s)	63, 450, 712
Bsu15I	ATCGAT	1 cut(s)	678
BsuRI	GGCC	3 cut(s)	147, 320, 635
BsuTUI	ATCGAT	1 cut(s)	678
BtgI	CCRYGG	1 cut(s)	751
BtsCI	GGATG	4 cut(s)	238, 494, 616, 793
BveI	ACCTGC	1 cut(s)	24
Cac8I	GCNNGC	2 cut(s)	618, 685
Cfr13I	GGNCC	2 cut(s)	318, 634
ClaI	ATCGAT	1 cut(s)	678
CseI	GACGC	1 cut(s)	247
Csp6I	GTAC	2 cut(s)	555, 847
CviAII	CATG	5 cut(s)	55, 322, 558, 752, 819
CviQI	GTAC	2 cut(s)	555, 847
DdeI	CTNAG	4 cut(s)	301, 408, 795, 924
DpnI	GATC	2 cut(s)	50, 398
DpnII	GATC	2 cut(s)	48, 396
EaeI	YGGCCR	1 cut(s)	145
Eam1104I	CTCTTC	1 cut(s)	282
EarI	CTCTTC	1 cut(s)	282
Ecl136II	GAGCTC	1 cut(s)	418
Eco130I	CCWWGG	2 cut(s)	448, 751
Eco24I	GRGCYC	1 cut(s)	420
Eco53kI	GAGCTC	1 cut(s)	418
Eco57I	CTGAAG	1 cut(s)	900
EcoICRI	GAGCTC	1 cut(s)	418
EcoO109I	RGGNCCY	1 cut(s)	634
EcoT14I	CCWWGG	2 cut(s)	448, 751
EcoT22I	ATGCAT	1 cut(s)	106
EcoT38I	GRGCYC	1 cut(s)	420
ErhI	CCWWGG	2 cut(s)	448, 751
FaeI	CATG	5 cut(s)	58, 325, 561, 755, 822
FaqI	GGGAC	1 cut(s)	805
FatI	CATG	5 cut(s)	54, 321, 557, 751, 818
Fnu4HI	GCNGC	4 cut(s)	20, 356, 588, 873
FokI	GGATG	4 cut(s)	245, 481, 623, 800
FriOI	GRGCYC	1 cut(s)	420
Fsp4HI	GCNGC	4 cut(s)	20, 356, 588, 873
FspBI	CTAG	2 cut(s)	684, 806
GluI	GCNGC	4 cut(s)	20, 356, 588, 873
HaeIII	GGCC	3 cut(s)	147, 320, 635
HapII	CCGG	1 cut(s)	726
HgaI	GACGC	1 cut(s)	247
Hin1I	GRCGYC	1 cut(s)	162
Hin1II	CATG	5 cut(s)	58, 325, 561, 755, 822
HinfI	GANTC	3 cut(s)	218, 311, 742
HpaII	CCGG	1 cut(s)	726
HphI	GGTGA	2 cut(s)	452, 715
Hpy188I	TCNGA	4 cut(s)	274, 304, 409, 694
Hpy188III	TCNNGA	2 cut(s)	187, 746
Hpy99I	CGWCG	1 cut(s)	167
HpyAV	CCTTC	5 cut(s)	166, 358, 565, 647, 875
HpyCH4III	ACNGT	2 cut(s)	512, 545
HpyCH4IV	ACGT	1 cut(s)	162
HpyCH4V	TGCA	9 cut(s)	19, 97, 104, 236, 587, 620, 827, 859, 875
HpyF10VI	GCNNNNNNNGC	5 cut(s)	346, 355, 584, 680, 905
HpyF3I	CTNAG	4 cut(s)	301, 408, 795, 924
HpySE526I	ACGT	1 cut(s)	162
Hsp92I	GRCGYC	1 cut(s)	162
Hsp92II	CATG	5 cut(s)	58, 325, 561, 755, 822
Kzo9I	GATC	2 cut(s)	48, 396
LmnI	GCTCC	1 cut(s)	62
LpnPI	CCDG	9 cut(s)	29, 372, 423, 585, 630, 675, 739, 828, 891
Lsp1109I	GCAGC	3 cut(s)	31, 599, 859
LweI	GCATC	6 cut(s)	91, 113, 223, 319, 466, 670
MaeI	CTAG	2 cut(s)	684, 806
MaeII	ACGT	1 cut(s)	162
MaeIII	GTNAC	4 cut(s)	369, 428, 560, 757
MalI	GATC	2 cut(s)	50, 398
MboI	GATC	2 cut(s)	48, 396
MboII	GAAGA	6 cut(s)	63, 213, 299, 455, 712, 823
MhlI	GDGCHC	1 cut(s)	420
MluCI	AATT	5 cut(s)	251, 266, 392, 529, 701
MlyI	GAGTC	1 cut(s)	751
MmeI	TCCRAC	3 cut(s)	522, 717, 748
MnlI	CCTC	6 cut(s)	62, 310, 451, 601, 778, 919
Mph1103I	ATGCAT	1 cut(s)	106
MseI	TTAA	3 cut(s)	195, 254, 467
MslI	CAYNNNNRTG	1 cut(s)	854
MspA1I	CMGCKG	3 cut(s)	437, 647, 764
MspI	CCGG	1 cut(s)	726
Mva1269I	GAATGC	1 cut(s)	861
MwoI	GCNNNNNNNGC	5 cut(s)	346, 355, 584, 680, 905
NcoI	CCATGG	1 cut(s)	751
NdeII	GATC	2 cut(s)	48, 396
NheI	GCTAGC	1 cut(s)	683
NlaIII	CATG	5 cut(s)	58, 325, 561, 755, 822
NlaIV	GGNNCC	1 cut(s)	691
NmuCI	GTSAC	1 cut(s)	428
NsiI	ATGCAT	1 cut(s)	106
NspI	RCATGY	1 cut(s)	561
PciI	ACATGT	1 cut(s)	557
PcsI	WCGNNNNNNNCGW	1 cut(s)	145
PctI	GAATGC	1 cut(s)	861
PfeI	GAWTC	2 cut(s)	218, 311
PkrI	GCNGC	4 cut(s)	21, 357, 589, 874
PleI	GAGTC	1 cut(s)	750
PpsI	GAGTC	1 cut(s)	750
PscI	ACATGT	1 cut(s)	557
Psp124BI	GAGCTC	1 cut(s)	420
PspN4I	GGNNCC	1 cut(s)	691
PspPI	GGNCC	2 cut(s)	318, 634
PstI	CTGCAG	1 cut(s)	21
RsaI	GTAC	2 cut(s)	556, 848
RsaNI	GTAC	2 cut(s)	555, 847
RseI	CAYNNNNRTG	1 cut(s)	854
SacI	GAGCTC	1 cut(s)	420
SaqAI	TTAA	3 cut(s)	195, 254, 467
SatI	GCNGC	4 cut(s)	20, 356, 588, 873
Sau3AI	GATC	2 cut(s)	48, 396
Sau96I	GGNCC	2 cut(s)	318, 634
SchI	GAGTC	1 cut(s)	751
SduI	GDGCHC	1 cut(s)	420
SfaNI	GCATC	6 cut(s)	91, 113, 223, 319, 466, 670
SfcI	CTRYAG	1 cut(s)	17
SmiMI	CAYNNNNRTG	1 cut(s)	854
SmlI	CTYRAG	1 cut(s)	75
SmoI	CTYRAG	1 cut(s)	75
Sse9I	AATT	5 cut(s)	251, 266, 392, 529, 701
SsiI	CCGC	5 cut(s)	356, 435, 645, 687, 762
SspMI	CTAG	2 cut(s)	684, 806
SstI	GAGCTC	1 cut(s)	420
StyI	CCWWGG	2 cut(s)	448, 751
TaaI	ACNGT	2 cut(s)	512, 545
TaiI	ACGT	1 cut(s)	165
TaqI	TCGA	2 cut(s)	165, 678
TasI	AATT	5 cut(s)	251, 266, 392, 529, 701
TauI	GCSGC	1 cut(s)	358
TfiI	GAWTC	2 cut(s)	218, 311
Tru1I	TTAA	3 cut(s)	195, 254, 467
Tru9I	TTAA	3 cut(s)	195, 254, 467
TseFI	GTSAC	1 cut(s)	428
TseI	GCWGC	3 cut(s)	19, 587, 872
Tsp45I	GTSAC	1 cut(s)	428
TspDTI	ATGAA	3 cut(s)	300, 338, 483
TspGWI	ACGGA	1 cut(s)	658
XceI	RCATGY	1 cut(s)	561
XspI	CTAG	2 cut(s)	684, 806
ZraI	GACGTC	1 cut(s)	163
Zsp2I	ATGCAT	1 cut(s)	106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.