Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 533 |
| AciI | CCGC | 2 cut(s) | 783, 969 |
| AclWI | GGATC | 1 cut(s) | 793 |
| AcoI | YGGCCR | 1 cut(s) | 697 |
| AcsI | RAATTY | 1 cut(s) | 284 |
| AcuI | CTGAAG | 1 cut(s) | 51 |
| AfaI | GTAC | 2 cut(s) | 116, 652 |
| AfeI | AGCGCT | 1 cut(s) | 176 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 52, 638, 639, 739 |
| AflIII | ACRYGT | 1 cut(s) | 142 |
| AgsI | TTSAA | 5 cut(s) | 181, 190, 256, 310, 346 |
| AjiI | CACGTC | 1 cut(s) | 143 |
| AluBI | AGCT | 4 cut(s) | 104, 238, 481, 520 |
| AluI | AGCT | 4 cut(s) | 104, 238, 481, 520 |
| Alw21I | GWGCWC | 1 cut(s) | 925 |
| Alw26I | GTCTC | 2 cut(s) | 92, 379 |
| Alw44I | GTGCAC | 1 cut(s) | 921 |
| AlwI | GGATC | 1 cut(s) | 793 |
| Ama87I | CYCGRG | 1 cut(s) | 295 |
| Aor51HI | AGCGCT | 1 cut(s) | 176 |
| AoxI | GGCC | 3 cut(s) | 421, 697, 741 |
| ApaLI | GTGCAC | 1 cut(s) | 921 |
| ApeKI | GCWGC | 2 cut(s) | 458, 683 |
| ApoI | RAATTY | 1 cut(s) | 284 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 566, 598 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 176 |
| AspLEI | GCGC | 2 cut(s) | 177, 731 |
| AspS9I | GGNCC | 3 cut(s) | 43, 415, 824 |
| AsuHPI | GGTGA | 3 cut(s) | 554, 916, 944 |
| AsuNHI | GCTAGC | 1 cut(s) | 779 |
| AvaI | CYCGRG | 1 cut(s) | 295 |
| AvaII | GGWCC | 3 cut(s) | 43, 415, 824 |
| BaeGI | GKGCMC | 2 cut(s) | 925, 969 |
| BalI | TGGCCA | 1 cut(s) | 699 |
| BbsI | GAAGAC | 2 cut(s) | 57, 552 |
| Bbv12I | GWGCWC | 1 cut(s) | 925 |
| BbvI | GCAGC | 2 cut(s) | 470, 695 |
| BccI | CCATC | 3 cut(s) | 121, 565, 641 |
| BceAI | ACGGC | 1 cut(s) | 728 |
| BcoDI | GTCTC | 2 cut(s) | 92, 379 |
| BfaI | CTAG | 2 cut(s) | 780, 842 |
| BfoI | RGCGCY | 2 cut(s) | 178, 732 |
| BisI | GCNGC | 2 cut(s) | 459, 684 |
| BlsI | GCNGC | 2 cut(s) | 460, 685 |
| Bme18I | GGWCC | 3 cut(s) | 43, 415, 824 |
| BmeT110I | CYCGRG | 1 cut(s) | 295 |
| BmgBI | CACGTC | 1 cut(s) | 143 |
| BmgT120I | GGNCC | 3 cut(s) | 43, 415, 824 |
| BmiI | GGNNCC | 2 cut(s) | 825, 1002 |
| BmsI | GCATC | 4 cut(s) | 36, 85, 568, 766 |
| BmtI | GCTAGC | 1 cut(s) | 783 |
| BpiI | GAAGAC | 2 cut(s) | 57, 552 |
| BpmI | CTGGAG | 1 cut(s) | 403 |
| Bpu10I | CCTNAGC | 1 cut(s) | 59 |
| BpuEI | CTTGAG | 1 cut(s) | 722 |
| Bsa29I | ATCGAT | 1 cut(s) | 304 |
| BsaJI | CCNNGG | 5 cut(s) | 418, 462, 550, 847, 996 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 52, 638, 639, 739 |
| Bse118I | RCCGGY | 1 cut(s) | 367 |
| Bse1I | ACTGG | 4 cut(s) | 386, 493, 700, 796 |
| Bse3DI | GCAATG | 3 cut(s) | 128, 664, 990 |
| BseCI | ATCGAT | 1 cut(s) | 304 |
| BseDI | CCNNGG | 5 cut(s) | 418, 462, 550, 847, 996 |
| BseGI | GGATG | 2 cut(s) | 132, 437 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 52, 638, 639, 739 |
| BseMI | GCAATG | 3 cut(s) | 128, 664, 990 |
| BseMII | CTCAG | 4 cut(s) | 462, 491, 501, 609 |
| BseNI | ACTGG | 4 cut(s) | 386, 493, 700, 796 |
| BseSI | GKGCMC | 2 cut(s) | 925, 969 |
| BseXI | GCAGC | 2 cut(s) | 470, 695 |
| BshFI | GGCC | 3 cut(s) | 423, 699, 743 |
| BshVI | ATCGAT | 1 cut(s) | 304 |
| BsiHKAI | GWGCWC | 1 cut(s) | 925 |
| BsiHKCI | CYCGRG | 1 cut(s) | 295 |
| BsiSI | CCGG | 1 cut(s) | 368 |
| BslFI | GGGAC | 2 cut(s) | 837, 901 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 52, 638, 639, 739 |
| BsmAI | GTCTC | 2 cut(s) | 92, 379 |
| BsmFI | GGGAC | 2 cut(s) | 837, 901 |
| BsmI | GAATGC | 2 cut(s) | 443, 957 |
| BsnI | GGCC | 3 cut(s) | 423, 699, 743 |
| BsoBI | CYCGRG | 1 cut(s) | 295 |
| Bsp1286I | GDGCHC | 2 cut(s) | 925, 969 |
| Bsp143I | GATC | 1 cut(s) | 785 |
| Bsp19I | CCATGG | 3 cut(s) | 462, 847, 996 |
| BspACI | CCGC | 2 cut(s) | 783, 969 |
| BspANI | GGCC | 3 cut(s) | 423, 699, 743 |
| BspCNI | CTCAG | 4 cut(s) | 461, 490, 502, 610 |
| BspDI | ATCGAT | 1 cut(s) | 304 |
| BspLI | GGNNCC | 2 cut(s) | 825, 1002 |
| BspOI | GCTAGC | 1 cut(s) | 783 |
| BspPI | GGATC | 1 cut(s) | 793 |
| BsrDI | GCAATG | 3 cut(s) | 128, 664, 990 |
| BsrFI | RCCGGY | 1 cut(s) | 367 |
| BsrI | ACTGG | 4 cut(s) | 386, 493, 700, 796 |
| BssAI | RCCGGY | 1 cut(s) | 367 |
| BssECI | CCNNGG | 5 cut(s) | 418, 462, 550, 847, 996 |
| BssMI | GATC | 1 cut(s) | 785 |
| BssT1I | CCWWGG | 5 cut(s) | 418, 462, 550, 847, 996 |
| Bst4CI | ACNGT | 2 cut(s) | 364, 530 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 680 |
| BstC8I | GCNNGC | 4 cut(s) | 369, 676, 781, 969 |
| BstDEI | CTNAG | 8 cut(s) | 59, 448, 477, 510, 611, 618, 663, 891 |
| BstDSI | CCRYGG | 3 cut(s) | 462, 847, 996 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 737 |
| BstF5I | GGATG | 2 cut(s) | 132, 437 |
| BstH2I | RGCGCY | 2 cut(s) | 178, 732 |
| BstHHI | GCGC | 2 cut(s) | 177, 731 |
| BstKTI | GATC | 1 cut(s) | 788 |
| BstMAI | GTCTC | 2 cut(s) | 92, 379 |
| BstMBI | GATC | 1 cut(s) | 785 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 449, 458, 680, 776 |
| BstNSI | RCATGY | 1 cut(s) | 657 |
| BstSLI | GKGCMC | 2 cut(s) | 925, 969 |
| BstV1I | GCAGC | 2 cut(s) | 470, 695 |
| BstV2I | GAAGAC | 2 cut(s) | 57, 552 |
| BstX2I | RGATCY | 1 cut(s) | 785 |
| BstYI | RGATCY | 1 cut(s) | 785 |
| Bsu15I | ATCGAT | 1 cut(s) | 304 |
| BsuRI | GGCC | 3 cut(s) | 423, 699, 743 |
| BsuTUI | ATCGAT | 1 cut(s) | 304 |
| BtgI | CCRYGG | 3 cut(s) | 462, 847, 996 |
| BtrI | CACGTC | 1 cut(s) | 143 |
| BtsCI | GGATG | 2 cut(s) | 132, 437 |
| BtsIMutI | CAGTG | 2 cut(s) | 615, 883 |
| Cac8I | GCNNGC | 4 cut(s) | 369, 676, 781, 969 |
| CfoI | GCGC | 2 cut(s) | 177, 731 |
| Cfr10I | RCCGGY | 1 cut(s) | 367 |
| Cfr13I | GGNCC | 3 cut(s) | 43, 415, 824 |
| ClaI | ATCGAT | 1 cut(s) | 304 |
| Csp6I | GTAC | 2 cut(s) | 115, 651 |
| CviQI | GTAC | 2 cut(s) | 115, 651 |
| DdeI | CTNAG | 8 cut(s) | 59, 448, 477, 510, 611, 618, 663, 891 |
| DpnI | GATC | 1 cut(s) | 787 |
| DpnII | GATC | 1 cut(s) | 785 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 533 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 533 |
| EaeI | YGGCCR | 1 cut(s) | 697 |
| Eco130I | CCWWGG | 5 cut(s) | 418, 462, 550, 847, 996 |
| Eco47I | GGWCC | 3 cut(s) | 43, 415, 824 |
| Eco47III | AGCGCT | 1 cut(s) | 176 |
| Eco57I | CTGAAG | 1 cut(s) | 51 |
| Eco88I | CYCGRG | 1 cut(s) | 295 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 737 |
| EcoRI | GAATTC | 1 cut(s) | 284 |
| EcoT14I | CCWWGG | 5 cut(s) | 418, 462, 550, 847, 996 |
| EcoT22I | ATGCAT | 3 cut(s) | 29, 100, 583 |
| ErhI | CCWWGG | 5 cut(s) | 418, 462, 550, 847, 996 |
| FaqI | GGGAC | 2 cut(s) | 837, 901 |
| FauI | CCCGC | 1 cut(s) | 976 |
| Fnu4HI | GCNGC | 2 cut(s) | 459, 684 |
| FokI | GGATG | 2 cut(s) | 139, 444 |
| Fsp4HI | GCNGC | 2 cut(s) | 459, 684 |
| FspBI | CTAG | 2 cut(s) | 780, 842 |
| GlaI | GCGC | 2 cut(s) | 176, 730 |
| GluI | GCNGC | 2 cut(s) | 459, 684 |
| GsuI | CTGGAG | 1 cut(s) | 403 |
| HaeII | RGCGCY | 2 cut(s) | 178, 732 |
| HaeIII | GGCC | 3 cut(s) | 423, 699, 743 |
| HapII | CCGG | 1 cut(s) | 368 |
| HhaI | GCGC | 2 cut(s) | 177, 731 |
| Hin6I | GCGC | 2 cut(s) | 175, 729 |
| HinP1I | GCGC | 2 cut(s) | 175, 729 |
| HincII | GTYRAC | 1 cut(s) | 526 |
| HindII | GTYRAC | 1 cut(s) | 526 |
| HindIII | AAGCTT | 1 cut(s) | 236 |
| HinfI | GANTC | 3 cut(s) | 212, 256, 306 |
| HpaII | CCGG | 1 cut(s) | 368 |
| HphI | GGTGA | 3 cut(s) | 554, 916, 944 |
| Hpy166II | GTNNAC | 4 cut(s) | 9, 494, 526, 923 |
| Hpy188I | TCNGA | 3 cut(s) | 70, 511, 790 |
| Hpy188III | TCNNGA | 2 cut(s) | 181, 297 |
| Hpy8I | GTNNAC | 4 cut(s) | 9, 494, 526, 923 |
| Hpy99I | CGWCG | 1 cut(s) | 725 |
| HpyAV | CCTTC | 7 cut(s) | 160, 221, 461, 661, 743, 932, 971 |
| HpyCH4III | ACNGT | 2 cut(s) | 364, 530 |
| HpyCH4IV | ACGT | 1 cut(s) | 142 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 449, 458, 680, 776 |
| HpyF3I | CTNAG | 8 cut(s) | 59, 448, 477, 510, 611, 618, 663, 891 |
| HpySE526I | ACGT | 1 cut(s) | 142 |
| HspAI | GCGC | 2 cut(s) | 175, 729 |
| KroI | GCCGGC | 1 cut(s) | 367 |
| KroNI | GCCGGC | 1 cut(s) | 369 |
| Kzo9I | GATC | 1 cut(s) | 785 |
| LpnPI | CCDG | 8 cut(s) | 367, 381, 420, 474, 506, 681, 809, 987 |
| Lsp1109I | GCAGC | 2 cut(s) | 470, 695 |
| LweI | GCATC | 4 cut(s) | 36, 85, 568, 766 |
| MaeI | CTAG | 2 cut(s) | 780, 842 |
| MaeII | ACGT | 1 cut(s) | 142 |
| MaeIII | GTNAC | 3 cut(s) | 400, 853, 941 |
| MalI | GATC | 1 cut(s) | 787 |
| MboI | GATC | 1 cut(s) | 785 |
| MboII | GAAGA | 7 cut(s) | 57, 207, 557, 656, 716, 919, 947 |
| MflI | RGATCY | 1 cut(s) | 785 |
| MhlI | GDGCHC | 2 cut(s) | 925, 969 |
| MlsI | TGGCCA | 1 cut(s) | 699 |
| MluCI | AATT | 4 cut(s) | 245, 284, 471, 690 |
| MluNI | TGGCCA | 1 cut(s) | 699 |
| Mox20I | TGGCCA | 1 cut(s) | 699 |
| Mph1103I | ATGCAT | 3 cut(s) | 29, 100, 583 |
| MroNI | GCCGGC | 1 cut(s) | 367 |
| MroXI | GAANNNNTTC | 1 cut(s) | 176 |
| MscI | TGGCCA | 1 cut(s) | 699 |
| MslI | CAYNNNNRTG | 3 cut(s) | 705, 950, 995 |
| Msp20I | TGGCCA | 1 cut(s) | 699 |
| MspI | CCGG | 1 cut(s) | 368 |
| Mva1269I | GAATGC | 2 cut(s) | 443, 957 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 449, 458, 680, 776 |
| NaeI | GCCGGC | 1 cut(s) | 369 |
| NcoI | CCATGG | 3 cut(s) | 462, 847, 996 |
| NdeII | GATC | 1 cut(s) | 785 |
| NgoMIV | GCCGGC | 1 cut(s) | 367 |
| NheI | GCTAGC | 1 cut(s) | 779 |
| NlaIV | GGNNCC | 2 cut(s) | 825, 1002 |
| NsiI | ATGCAT | 3 cut(s) | 29, 100, 583 |
| NspI | RCATGY | 1 cut(s) | 657 |
| PaeR7I | CTCGAG | 1 cut(s) | 295 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 139 |
| PctI | GAATGC | 2 cut(s) | 443, 957 |
| PdiI | GCCGGC | 1 cut(s) | 369 |
| PdmI | GAANNNNTTC | 1 cut(s) | 176 |
| PfeI | GAWTC | 3 cut(s) | 212, 256, 306 |
| PkrI | GCNGC | 2 cut(s) | 460, 685 |
| PspN4I | GGNNCC | 2 cut(s) | 825, 1002 |
| PspPI | GGNCC | 3 cut(s) | 43, 415, 824 |
| PsuI | RGATCY | 1 cut(s) | 785 |
| RsaI | GTAC | 2 cut(s) | 116, 652 |
| RsaNI | GTAC | 2 cut(s) | 115, 651 |
| RseI | CAYNNNNRTG | 3 cut(s) | 705, 950, 995 |
| SatI | GCNGC | 2 cut(s) | 459, 684 |
| Sau3AI | GATC | 1 cut(s) | 785 |
| Sau96I | GGNCC | 3 cut(s) | 43, 415, 824 |
| SduI | GDGCHC | 2 cut(s) | 925, 969 |
| SfaNI | GCATC | 4 cut(s) | 36, 85, 568, 766 |
| Sfr274I | CTCGAG | 1 cut(s) | 295 |
| SinI | GGWCC | 3 cut(s) | 43, 415, 824 |
| SlaI | CTCGAG | 1 cut(s) | 295 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 705, 950, 995 |
| SmlI | CTYRAG | 2 cut(s) | 295, 737 |
| SmoI | CTYRAG | 2 cut(s) | 295, 737 |
| Sse9I | AATT | 4 cut(s) | 245, 284, 471, 690 |
| SsiI | CCGC | 2 cut(s) | 783, 969 |
| SspMI | CTAG | 2 cut(s) | 780, 842 |
| StyI | CCWWGG | 5 cut(s) | 418, 462, 550, 847, 996 |
| TaaI | ACNGT | 2 cut(s) | 364, 530 |
| TaiI | ACGT | 1 cut(s) | 145 |
| TaqI | TCGA | 2 cut(s) | 296, 304 |
| TasI | AATT | 4 cut(s) | 245, 284, 471, 690 |
| TfiI | GAWTC | 3 cut(s) | 212, 256, 306 |
| TscAI | CASTG | 2 cut(s) | 622, 883 |
| TseI | GCWGC | 2 cut(s) | 458, 683 |
| TspDTI | ATGAA | 7 cut(s) | 240, 277, 297, 333, 366, 580, 717 |
| TspRI | CASTG | 2 cut(s) | 622, 883 |
| VneI | GTGCAC | 1 cut(s) | 921 |
| VpaK11BI | GGWCC | 3 cut(s) | 43, 415, 824 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 737 |
| XapI | RAATTY | 1 cut(s) | 284 |
| XceI | RCATGY | 1 cut(s) | 657 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 425 |
| XhoI | CTCGAG | 1 cut(s) | 295 |
| XmnI | GAANNNNTTC | 1 cut(s) | 176 |
| XspI | CTAG | 2 cut(s) | 780, 842 |
| Zsp2I | ATGCAT | 3 cut(s) | 29, 100, 583 |