Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 533 |
| AciI | CCGC | 4 cut(s) | 82, 714, 858, 987 |
| AclWI | GGATC | 2 cut(s) | 37, 793 |
| AcoI | YGGCCR | 1 cut(s) | 697 |
| AcsI | RAATTY | 1 cut(s) | 480 |
| AcuI | CTGAAG | 1 cut(s) | 90 |
| AcyI | GRCGYC | 1 cut(s) | 873 |
| AfaI | GTAC | 4 cut(s) | 231, 616, 652, 944 |
| AfeI | AGCGCT | 1 cut(s) | 176 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 638 |
| AflII | CTTAAG | 1 cut(s) | 737 |
| AflIII | ACRYGT | 1 cut(s) | 142 |
| AgsI | TTSAA | 5 cut(s) | 190, 310, 346, 486, 807 |
| AjiI | CACGTC | 1 cut(s) | 143 |
| AluBI | AGCT | 2 cut(s) | 371, 398 |
| AluI | AGCT | 2 cut(s) | 371, 398 |
| Alw21I | GWGCWC | 1 cut(s) | 733 |
| Alw26I | GTCTC | 2 cut(s) | 92, 614 |
| Alw44I | GTGCAC | 1 cut(s) | 729 |
| AlwI | GGATC | 2 cut(s) | 37, 793 |
| Aor51HI | AGCGCT | 1 cut(s) | 176 |
| AoxI | GGCC | 3 cut(s) | 36, 421, 697 |
| ApaLI | GTGCAC | 1 cut(s) | 729 |
| ApeKI | GCWGC | 3 cut(s) | 458, 683, 968 |
| ApoI | RAATTY | 1 cut(s) | 480 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 237, 269 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 176 |
| AspLEI | GCGC | 1 cut(s) | 177 |
| AspS9I | GGNCC | 1 cut(s) | 415 |
| AsuHPI | GGTGA | 3 cut(s) | 554, 916, 944 |
| AvaII | GGWCC | 1 cut(s) | 415 |
| BaeGI | GKGCMC | 2 cut(s) | 714, 733 |
| BalI | TGGCCA | 1 cut(s) | 699 |
| BbsI | GAAGAC | 2 cut(s) | 57, 552 |
| Bbv12I | GWGCWC | 1 cut(s) | 733 |
| BbvI | GCAGC | 3 cut(s) | 470, 695, 955 |
| BccI | CCATC | 5 cut(s) | 121, 416, 565, 641, 878 |
| BcoDI | GTCTC | 2 cut(s) | 92, 614 |
| BfaI | CTAG | 3 cut(s) | 53, 780, 842 |
| BfoI | RGCGCY | 1 cut(s) | 178 |
| BfrI | CTTAAG | 1 cut(s) | 737 |
| BisI | GCNGC | 3 cut(s) | 459, 684, 969 |
| BlsI | GCNGC | 3 cut(s) | 460, 685, 970 |
| Bme18I | GGWCC | 1 cut(s) | 415 |
| BmgBI | CACGTC | 1 cut(s) | 143 |
| BmgT120I | GGNCC | 1 cut(s) | 415 |
| BmsI | GCATC | 4 cut(s) | 85, 422, 568, 766 |
| BpiI | GAAGAC | 2 cut(s) | 57, 552 |
| BpmI | CTGGAG | 1 cut(s) | 403 |
| BpuEI | CTTGAG | 1 cut(s) | 280 |
| Bsa29I | ATCGAT | 1 cut(s) | 304 |
| BsaHI | GRCGYC | 1 cut(s) | 873 |
| BsaJI | CCNNGG | 3 cut(s) | 418, 462, 550 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 638 |
| Bse1I | ACTGG | 6 cut(s) | 16, 386, 493, 643, 700, 796 |
| Bse3DI | GCAATG | 4 cut(s) | 105, 128, 156, 664 |
| BseCI | ATCGAT | 1 cut(s) | 304 |
| BseDI | CCNNGG | 3 cut(s) | 418, 462, 550 |
| BseGI | GGATG | 3 cut(s) | 132, 437, 580 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 638 |
| BseMI | GCAATG | 4 cut(s) | 105, 128, 156, 664 |
| BseMII | CTCAG | 1 cut(s) | 462 |
| BseNI | ACTGG | 6 cut(s) | 16, 386, 493, 643, 700, 796 |
| BseSI | GKGCMC | 2 cut(s) | 714, 733 |
| BseXI | GCAGC | 3 cut(s) | 470, 695, 955 |
| BsgI | GTGCAG | 1 cut(s) | 954 |
| BshFI | GGCC | 3 cut(s) | 38, 423, 699 |
| BshVI | ATCGAT | 1 cut(s) | 304 |
| BsiHKAI | GWGCWC | 1 cut(s) | 733 |
| BslFI | GGGAC | 1 cut(s) | 901 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 638 |
| BsmAI | GTCTC | 2 cut(s) | 92, 614 |
| BsmFI | GGGAC | 1 cut(s) | 901 |
| BsmI | GAATGC | 1 cut(s) | 957 |
| BsnI | GGCC | 3 cut(s) | 38, 423, 699 |
| Bsp1286I | GDGCHC | 2 cut(s) | 714, 733 |
| Bsp143I | GATC | 3 cut(s) | 42, 785, 844 |
| Bsp19I | CCATGG | 1 cut(s) | 462 |
| BspACI | CCGC | 4 cut(s) | 82, 714, 858, 987 |
| BspANI | GGCC | 3 cut(s) | 38, 423, 699 |
| BspCNI | CTCAG | 1 cut(s) | 461 |
| BspDI | ATCGAT | 1 cut(s) | 304 |
| BspPI | GGATC | 2 cut(s) | 37, 793 |
| BspTI | CTTAAG | 1 cut(s) | 737 |
| BsrDI | GCAATG | 4 cut(s) | 105, 128, 156, 664 |
| BsrI | ACTGG | 6 cut(s) | 16, 386, 493, 643, 700, 796 |
| BssECI | CCNNGG | 3 cut(s) | 418, 462, 550 |
| BssMI | GATC | 3 cut(s) | 42, 785, 844 |
| BssNI | GRCGYC | 1 cut(s) | 873 |
| BssT1I | CCWWGG | 3 cut(s) | 418, 462, 550 |
| Bst4CI | ACNGT | 3 cut(s) | 364, 530, 722 |
| Bst6I | CTCTTC | 1 cut(s) | 276 |
| BstACI | GRCGYC | 1 cut(s) | 873 |
| BstAFI | CTTAAG | 1 cut(s) | 737 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 680 |
| BstC8I | GCNNGC | 2 cut(s) | 369, 714 |
| BstDEI | CTNAG | 3 cut(s) | 448, 611, 891 |
| BstDSI | CCRYGG | 1 cut(s) | 462 |
| BstF5I | GGATG | 3 cut(s) | 132, 437, 580 |
| BstH2I | RGCGCY | 1 cut(s) | 178 |
| BstHHI | GCGC | 1 cut(s) | 177 |
| BstKTI | GATC | 3 cut(s) | 45, 788, 847 |
| BstMAI | GTCTC | 2 cut(s) | 92, 614 |
| BstMBI | GATC | 3 cut(s) | 42, 785, 844 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 449, 458, 680, 776, 1001 |
| BstNSI | RCATGY | 1 cut(s) | 657 |
| BstSLI | GKGCMC | 2 cut(s) | 714, 733 |
| BstV1I | GCAGC | 3 cut(s) | 470, 695, 955 |
| BstV2I | GAAGAC | 2 cut(s) | 57, 552 |
| BstX2I | RGATCY | 1 cut(s) | 785 |
| BstYI | RGATCY | 1 cut(s) | 785 |
| Bsu15I | ATCGAT | 1 cut(s) | 304 |
| BsuRI | GGCC | 3 cut(s) | 38, 423, 699 |
| BsuTUI | ATCGAT | 1 cut(s) | 304 |
| BtgI | CCRYGG | 1 cut(s) | 462 |
| BtrI | CACGTC | 1 cut(s) | 143 |
| BtsCI | GGATG | 3 cut(s) | 132, 437, 580 |
| BtsI | GCAGTG | 2 cut(s) | 459, 1014 |
| BtsIMutI | CAGTG | 5 cut(s) | 23, 459, 727, 883, 1014 |
| Cac8I | GCNNGC | 2 cut(s) | 369, 714 |
| CfoI | GCGC | 1 cut(s) | 177 |
| Cfr13I | GGNCC | 1 cut(s) | 415 |
| ClaI | ATCGAT | 1 cut(s) | 304 |
| CseI | GACGC | 1 cut(s) | 862 |
| Csp6I | GTAC | 4 cut(s) | 230, 615, 651, 943 |
| CviAII | CATG | 9 cut(s) | 49, 317, 463, 605, 654, 680, 701, 848, 915 |
| CviQI | GTAC | 4 cut(s) | 230, 615, 651, 943 |
| DdeI | CTNAG | 3 cut(s) | 448, 611, 891 |
| DpnI | GATC | 3 cut(s) | 44, 787, 846 |
| DpnII | GATC | 3 cut(s) | 42, 785, 844 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 533 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 533 |
| EaeI | YGGCCR | 1 cut(s) | 697 |
| Eam1104I | CTCTTC | 1 cut(s) | 276 |
| EarI | CTCTTC | 1 cut(s) | 276 |
| Eco130I | CCWWGG | 3 cut(s) | 418, 462, 550 |
| Eco47I | GGWCC | 1 cut(s) | 415 |
| Eco47III | AGCGCT | 1 cut(s) | 176 |
| Eco57I | CTGAAG | 1 cut(s) | 90 |
| EcoT14I | CCWWGG | 3 cut(s) | 418, 462, 550 |
| ErhI | CCWWGG | 3 cut(s) | 418, 462, 550 |
| FaeI | CATG | 9 cut(s) | 52, 320, 466, 608, 657, 683, 704, 851, 918 |
| FaqI | GGGAC | 1 cut(s) | 901 |
| FatI | CATG | 9 cut(s) | 48, 316, 462, 604, 653, 679, 700, 847, 914 |
| FauI | CCCGC | 2 cut(s) | 89, 721 |
| Fnu4HI | GCNGC | 3 cut(s) | 459, 684, 969 |
| FokI | GGATG | 3 cut(s) | 139, 444, 587 |
| Fsp4HI | GCNGC | 3 cut(s) | 459, 684, 969 |
| FspBI | CTAG | 3 cut(s) | 53, 780, 842 |
| GlaI | GCGC | 1 cut(s) | 176 |
| GluI | GCNGC | 3 cut(s) | 459, 684, 969 |
| GsuI | CTGGAG | 1 cut(s) | 403 |
| HaeII | RGCGCY | 1 cut(s) | 178 |
| HaeIII | GGCC | 3 cut(s) | 38, 423, 699 |
| HgaI | GACGC | 1 cut(s) | 862 |
| HhaI | GCGC | 1 cut(s) | 177 |
| Hin1I | GRCGYC | 1 cut(s) | 873 |
| Hin1II | CATG | 9 cut(s) | 52, 320, 466, 608, 657, 683, 704, 851, 918 |
| Hin6I | GCGC | 1 cut(s) | 175 |
| HinP1I | GCGC | 1 cut(s) | 175 |
| HinfI | GANTC | 3 cut(s) | 212, 306, 403 |
| HphI | GGTGA | 3 cut(s) | 554, 916, 944 |
| Hpy166II | GTNNAC | 4 cut(s) | 232, 288, 526, 731 |
| Hpy188I | TCNGA | 3 cut(s) | 70, 511, 790 |
| Hpy188III | TCNNGA | 1 cut(s) | 297 |
| Hpy8I | GTNNAC | 4 cut(s) | 232, 288, 526, 731 |
| Hpy99I | CGWCG | 1 cut(s) | 227 |
| HpyAV | CCTTC | 5 cut(s) | 160, 461, 661, 932, 971 |
| HpyCH4III | ACNGT | 3 cut(s) | 364, 530, 722 |
| HpyCH4IV | ACGT | 1 cut(s) | 142 |
| HpyCH4V | TGCA | 8 cut(s) | 21, 98, 266, 657, 683, 731, 955, 971 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 449, 458, 680, 776, 1001 |
| HpyF3I | CTNAG | 3 cut(s) | 448, 611, 891 |
| HpySE526I | ACGT | 1 cut(s) | 142 |
| Hsp92I | GRCGYC | 1 cut(s) | 873 |
| Hsp92II | CATG | 9 cut(s) | 52, 320, 466, 608, 657, 683, 704, 851, 918 |
| HspAI | GCGC | 1 cut(s) | 175 |
| Kzo9I | GATC | 3 cut(s) | 42, 785, 844 |
| LmnI | GCTCC | 1 cut(s) | 395 |
| LpnPI | CCDG | 9 cut(s) | 29, 367, 381, 474, 624, 681, 807, 809, 959 |
| Lsp1109I | GCAGC | 3 cut(s) | 470, 695, 955 |
| LweI | GCATC | 4 cut(s) | 85, 422, 568, 766 |
| MaeI | CTAG | 3 cut(s) | 53, 780, 842 |
| MaeII | ACGT | 1 cut(s) | 142 |
| MaeIII | GTNAC | 3 cut(s) | 358, 400, 853 |
| MalI | GATC | 3 cut(s) | 44, 787, 846 |
| MboI | GATC | 3 cut(s) | 42, 785, 844 |
| MboII | GAAGA | 7 cut(s) | 57, 207, 293, 557, 716, 919, 947 |
| MflI | RGATCY | 1 cut(s) | 785 |
| MhlI | GDGCHC | 2 cut(s) | 714, 733 |
| MlsI | TGGCCA | 1 cut(s) | 699 |
| MluCI | AATT | 5 cut(s) | 245, 471, 480, 494, 625 |
| MluNI | TGGCCA | 1 cut(s) | 699 |
| MlyI | GAGTC | 1 cut(s) | 397 |
| MmeI | TCCRAC | 1 cut(s) | 243 |
| MnlI | CCTC | 7 cut(s) | 56, 304, 378, 553, 557, 697, 1015 |
| Mox20I | TGGCCA | 1 cut(s) | 699 |
| MroXI | GAANNNNTTC | 1 cut(s) | 176 |
| MscI | TGGCCA | 1 cut(s) | 699 |
| MseI | TTAA | 3 cut(s) | 248, 738, 1021 |
| MslI | CAYNNNNRTG | 2 cut(s) | 705, 950 |
| Msp20I | TGGCCA | 1 cut(s) | 699 |
| MspA1I | CMGCKG | 1 cut(s) | 860 |
| MspCI | CTTAAG | 1 cut(s) | 737 |
| Mva1269I | GAATGC | 1 cut(s) | 957 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 449, 458, 680, 776, 1001 |
| NcoI | CCATGG | 1 cut(s) | 462 |
| NdeII | GATC | 3 cut(s) | 42, 785, 844 |
| NlaIII | CATG | 9 cut(s) | 52, 320, 466, 608, 657, 683, 704, 851, 918 |
| NmeAIII | GCCGAG | 1 cut(s) | 187 |
| NmuCI | GTSAC | 1 cut(s) | 400 |
| NspI | RCATGY | 1 cut(s) | 657 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 139 |
| PctI | GAATGC | 1 cut(s) | 957 |
| PdmI | GAANNNNTTC | 1 cut(s) | 176 |
| PfeI | GAWTC | 2 cut(s) | 212, 306 |
| PkrI | GCNGC | 3 cut(s) | 460, 685, 970 |
| PleI | GAGTC | 1 cut(s) | 397 |
| PpsI | GAGTC | 1 cut(s) | 397 |
| PspPI | GGNCC | 1 cut(s) | 415 |
| PsuI | RGATCY | 1 cut(s) | 785 |
| RsaI | GTAC | 4 cut(s) | 231, 616, 652, 944 |
| RsaNI | GTAC | 4 cut(s) | 230, 615, 651, 943 |
| RseI | CAYNNNNRTG | 2 cut(s) | 705, 950 |
| SaqAI | TTAA | 3 cut(s) | 248, 738, 1021 |
| SatI | GCNGC | 3 cut(s) | 459, 684, 969 |
| Sau3AI | GATC | 3 cut(s) | 42, 785, 844 |
| Sau96I | GGNCC | 1 cut(s) | 415 |
| SchI | GAGTC | 1 cut(s) | 397 |
| SduI | GDGCHC | 2 cut(s) | 714, 733 |
| SetI | ASST | 7 cut(s) | 145, 241, 273, 373, 400, 760, 982 |
| SfaNI | GCATC | 4 cut(s) | 85, 422, 568, 766 |
| SinI | GGWCC | 1 cut(s) | 415 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 705, 950 |
| SmlI | CTYRAG | 2 cut(s) | 295, 737 |
| SmoI | CTYRAG | 2 cut(s) | 295, 737 |
| Sse9I | AATT | 5 cut(s) | 245, 471, 480, 494, 625 |
| SsiI | CCGC | 4 cut(s) | 82, 714, 858, 987 |
| SspI | AATATT | 1 cut(s) | 186 |
| SspMI | CTAG | 3 cut(s) | 53, 780, 842 |
| StyI | CCWWGG | 3 cut(s) | 418, 462, 550 |
| TaaI | ACNGT | 3 cut(s) | 364, 530, 722 |
| TaiI | ACGT | 1 cut(s) | 145 |
| TaqI | TCGA | 2 cut(s) | 304, 689 |
| TasI | AATT | 5 cut(s) | 245, 471, 480, 494, 625 |
| TatI | WGTACW | 1 cut(s) | 229 |
| TfiI | GAWTC | 2 cut(s) | 212, 306 |
| Tru1I | TTAA | 3 cut(s) | 248, 738, 1021 |
| Tru9I | TTAA | 3 cut(s) | 248, 738, 1021 |
| TscAI | CASTG | 5 cut(s) | 23, 459, 727, 883, 1021 |
| TseFI | GTSAC | 1 cut(s) | 400 |
| TseI | GCWGC | 3 cut(s) | 458, 683, 968 |
| Tsp45I | GTSAC | 1 cut(s) | 400 |
| TspDTI | ATGAA | 5 cut(s) | 294, 333, 366, 580, 717 |
| TspRI | CASTG | 5 cut(s) | 23, 459, 727, 883, 1021 |
| Vha464I | CTTAAG | 1 cut(s) | 737 |
| VneI | GTGCAC | 1 cut(s) | 729 |
| VpaK11BI | GGWCC | 1 cut(s) | 415 |
| XapI | RAATTY | 1 cut(s) | 480 |
| XceI | RCATGY | 1 cut(s) | 657 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 425 |
| XmnI | GAANNNNTTC | 1 cut(s) | 176 |
| XspI | CTAG | 3 cut(s) | 53, 780, 842 |