Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 515 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 620 |
| AccI | GTMKAC | 1 cut(s) | 150 |
| AciI | CCGC | 2 cut(s) | 297, 840 |
| AclWI | GGATC | 5 cut(s) | 40, 762, 775, 801, 814 |
| AcsI | RAATTY | 1 cut(s) | 287 |
| AfaI | GTAC | 4 cut(s) | 119, 598, 634, 878 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 55, 620 |
| AflII | CTTAAG | 1 cut(s) | 719 |
| AflIII | ACRYGT | 1 cut(s) | 145 |
| AgsI | TTSAA | 4 cut(s) | 184, 193, 313, 349 |
| AjiI | CACGTC | 1 cut(s) | 146 |
| AluBI | AGCT | 5 cut(s) | 374, 401, 502, 605, 986 |
| AluI | AGCT | 5 cut(s) | 374, 401, 502, 605, 986 |
| Alw21I | GWGCWC | 1 cut(s) | 715 |
| Alw26I | GTCTC | 1 cut(s) | 968 |
| Alw44I | GTGCAC | 1 cut(s) | 711 |
| AlwI | GGATC | 5 cut(s) | 40, 762, 775, 801, 814 |
| AoxI | GGCC | 5 cut(s) | 39, 75, 424, 679, 723 |
| ApaLI | GTGCAC | 1 cut(s) | 711 |
| ApeKI | GCWGC | 2 cut(s) | 440, 665 |
| ApoI | RAATTY | 1 cut(s) | 287 |
| AspS9I | GGNCC | 1 cut(s) | 418 |
| AsuHPI | GGTGA | 4 cut(s) | 536, 898, 914, 926 |
| AsuNHI | GCTAGC | 1 cut(s) | 362 |
| AvaII | GGWCC | 1 cut(s) | 418 |
| BaeGI | GKGCMC | 1 cut(s) | 715 |
| BamHI | GGATCC | 2 cut(s) | 767, 806 |
| BauI | CACGAG | 1 cut(s) | 50 |
| BbsI | GAAGAC | 1 cut(s) | 60 |
| Bbv12I | GWGCWC | 1 cut(s) | 715 |
| BbvI | GCAGC | 2 cut(s) | 452, 677 |
| BccI | CCATC | 5 cut(s) | 124, 419, 547, 623, 860 |
| BceAI | ACGGC | 2 cut(s) | 419, 710 |
| BcoDI | GTCTC | 1 cut(s) | 968 |
| BfaI | CTAG | 5 cut(s) | 363, 503, 762, 819, 824 |
| BfrI | CTTAAG | 1 cut(s) | 719 |
| BisI | GCNGC | 2 cut(s) | 441, 666 |
| BlsI | GCNGC | 2 cut(s) | 442, 667 |
| BmcAI | AGTACT | 1 cut(s) | 878 |
| Bme18I | GGWCC | 1 cut(s) | 418 |
| BmgBI | CACGTC | 1 cut(s) | 146 |
| BmgT120I | GGNCC | 1 cut(s) | 418 |
| BmiI | GGNNCC | 2 cut(s) | 769, 808 |
| BmsI | GCATC | 5 cut(s) | 88, 110, 550, 748, 924 |
| BmtI | GCTAGC | 1 cut(s) | 366 |
| BpiI | GAAGAC | 1 cut(s) | 60 |
| BpmI | CTGGAG | 1 cut(s) | 406 |
| BpuEI | CTTGAG | 1 cut(s) | 772 |
| BsaI | GGTCTC | 1 cut(s) | 968 |
| BsaJI | CCNNGG | 5 cut(s) | 421, 435, 444, 829, 978 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 55, 620 |
| Bse1I | ACTGG | 5 cut(s) | 389, 475, 625, 778, 884 |
| Bse3DI | GCAATG | 3 cut(s) | 131, 646, 972 |
| BseDI | CCNNGG | 5 cut(s) | 421, 435, 444, 829, 978 |
| BseGI | GGATG | 2 cut(s) | 135, 939 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 55, 620 |
| BseMI | GCAATG | 3 cut(s) | 131, 646, 972 |
| BseMII | CTCAG | 3 cut(s) | 63, 483, 607 |
| BseNI | ACTGG | 5 cut(s) | 389, 475, 625, 778, 884 |
| BseSI | GKGCMC | 1 cut(s) | 715 |
| BseXI | GCAGC | 2 cut(s) | 452, 677 |
| BseYI | CCCAGC | 1 cut(s) | 55 |
| BsgI | GTGCAG | 1 cut(s) | 936 |
| BshFI | GGCC | 5 cut(s) | 41, 77, 426, 681, 725 |
| BsiHKAI | GWGCWC | 1 cut(s) | 715 |
| BslFI | GGGAC | 1 cut(s) | 883 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 55, 620 |
| BsmAI | GTCTC | 1 cut(s) | 968 |
| BsmFI | GGGAC | 1 cut(s) | 883 |
| BsnI | GGCC | 5 cut(s) | 41, 77, 426, 681, 725 |
| Bso31I | GGTCTC | 1 cut(s) | 968 |
| Bsp1286I | GDGCHC | 1 cut(s) | 715 |
| Bsp143I | GATC | 4 cut(s) | 45, 529, 767, 806 |
| Bsp19I | CCATGG | 2 cut(s) | 829, 978 |
| BspACI | CCGC | 2 cut(s) | 297, 840 |
| BspANI | GGCC | 5 cut(s) | 41, 77, 426, 681, 725 |
| BspCNI | CTCAG | 3 cut(s) | 64, 484, 606 |
| BspLI | GGNNCC | 2 cut(s) | 769, 808 |
| BspOI | GCTAGC | 1 cut(s) | 366 |
| BspPI | GGATC | 5 cut(s) | 40, 762, 775, 801, 814 |
| BspTI | CTTAAG | 1 cut(s) | 719 |
| BspTNI | GGTCTC | 1 cut(s) | 968 |
| BsrDI | GCAATG | 3 cut(s) | 131, 646, 972 |
| BsrI | ACTGG | 5 cut(s) | 389, 475, 625, 778, 884 |
| BssECI | CCNNGG | 5 cut(s) | 421, 435, 444, 829, 978 |
| BssMI | GATC | 4 cut(s) | 45, 529, 767, 806 |
| BssSI | CACGAG | 1 cut(s) | 50 |
| BssT1I | CCWWGG | 3 cut(s) | 421, 829, 978 |
| Bst2BI | CACGAG | 1 cut(s) | 50 |
| Bst4CI | ACNGT | 2 cut(s) | 29, 512 |
| BstAFI | CTTAAG | 1 cut(s) | 719 |
| BstC8I | GCNNGC | 4 cut(s) | 364, 368, 372, 696 |
| BstDEI | CTNAG | 4 cut(s) | 72, 492, 593, 873 |
| BstDSI | CCRYGG | 4 cut(s) | 435, 444, 829, 978 |
| BstF5I | GGATG | 2 cut(s) | 135, 939 |
| BstKTI | GATC | 4 cut(s) | 48, 532, 770, 809 |
| BstMAI | GTCTC | 1 cut(s) | 968 |
| BstMBI | GATC | 4 cut(s) | 45, 529, 767, 806 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 440, 662, 758, 983 |
| BstNSI | RCATGY | 1 cut(s) | 639 |
| BstSLI | GKGCMC | 1 cut(s) | 715 |
| BstV1I | GCAGC | 2 cut(s) | 452, 677 |
| BstV2I | GAAGAC | 1 cut(s) | 60 |
| BstX2I | RGATCY | 2 cut(s) | 767, 806 |
| BstYI | RGATCY | 2 cut(s) | 767, 806 |
| BsuRI | GGCC | 5 cut(s) | 41, 77, 426, 681, 725 |
| BtgI | CCRYGG | 4 cut(s) | 435, 444, 829, 978 |
| BtgZI | GCGATG | 1 cut(s) | 876 |
| BtrI | CACGTC | 1 cut(s) | 146 |
| BtsCI | GGATG | 2 cut(s) | 135, 939 |
| BtsI | GCAGTG | 1 cut(s) | 996 |
| BtsIMutI | CAGTG | 2 cut(s) | 618, 996 |
| Cac8I | GCNNGC | 4 cut(s) | 364, 368, 372, 696 |
| Cfr13I | GGNCC | 1 cut(s) | 418 |
| CseI | GACGC | 1 cut(s) | 657 |
| Csp6I | GTAC | 4 cut(s) | 118, 597, 633, 877 |
| CviAII | CATG | 7 cut(s) | 160, 320, 587, 636, 830, 897, 979 |
| CviQI | GTAC | 4 cut(s) | 118, 597, 633, 877 |
| DdeI | CTNAG | 4 cut(s) | 72, 492, 593, 873 |
| DpnI | GATC | 4 cut(s) | 47, 531, 769, 808 |
| DpnII | GATC | 4 cut(s) | 45, 529, 767, 806 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 515 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 515 |
| Eco130I | CCWWGG | 3 cut(s) | 421, 829, 978 |
| Eco31I | GGTCTC | 1 cut(s) | 968 |
| Eco47I | GGWCC | 1 cut(s) | 418 |
| EcoRI | GAATTC | 1 cut(s) | 287 |
| EcoT14I | CCWWGG | 3 cut(s) | 421, 829, 978 |
| EcoT22I | ATGCAT | 1 cut(s) | 103 |
| ErhI | CCWWGG | 3 cut(s) | 421, 829, 978 |
| FaeI | CATG | 7 cut(s) | 163, 323, 590, 639, 833, 900, 982 |
| FaqI | GGGAC | 1 cut(s) | 883 |
| FatI | CATG | 7 cut(s) | 159, 319, 586, 635, 829, 896, 978 |
| FblI | GTMKAC | 1 cut(s) | 150 |
| Fnu4HI | GCNGC | 2 cut(s) | 441, 666 |
| FokI | GGATG | 2 cut(s) | 142, 946 |
| Fsp4HI | GCNGC | 2 cut(s) | 441, 666 |
| FspBI | CTAG | 5 cut(s) | 363, 503, 762, 819, 824 |
| GluI | GCNGC | 2 cut(s) | 441, 666 |
| GsaI | CCCAGC | 1 cut(s) | 59 |
| GsuI | CTGGAG | 1 cut(s) | 406 |
| HaeIII | GGCC | 5 cut(s) | 41, 77, 426, 681, 725 |
| HgaI | GACGC | 1 cut(s) | 657 |
| Hin1II | CATG | 7 cut(s) | 163, 323, 590, 639, 833, 900, 982 |
| HincII | GTYRAC | 1 cut(s) | 508 |
| HindII | GTYRAC | 1 cut(s) | 508 |
| HindIII | AAGCTT | 1 cut(s) | 984 |
| HinfI | GANTC | 3 cut(s) | 215, 259, 591 |
| HphI | GGTGA | 4 cut(s) | 536, 898, 914, 926 |
| Hpy166II | GTNNAC | 5 cut(s) | 9, 151, 508, 713, 905 |
| Hpy188I | TCNGA | 3 cut(s) | 73, 493, 772 |
| Hpy8I | GTNNAC | 5 cut(s) | 9, 151, 508, 713, 905 |
| Hpy99I | CGWCG | 1 cut(s) | 731 |
| HpyAV | CCTTC | 4 cut(s) | 443, 643, 914, 953 |
| HpyCH4III | ACNGT | 2 cut(s) | 29, 512 |
| HpyCH4IV | ACGT | 1 cut(s) | 145 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 440, 662, 758, 983 |
| HpyF3I | CTNAG | 4 cut(s) | 72, 492, 593, 873 |
| HpySE526I | ACGT | 1 cut(s) | 145 |
| Hsp92II | CATG | 7 cut(s) | 163, 323, 590, 639, 833, 900, 982 |
| Kzo9I | GATC | 4 cut(s) | 45, 529, 767, 806 |
| LmnI | GCTCC | 1 cut(s) | 398 |
| Lsp1109I | GCAGC | 2 cut(s) | 452, 677 |
| LweI | GCATC | 5 cut(s) | 88, 110, 550, 748, 924 |
| MaeI | CTAG | 5 cut(s) | 363, 503, 762, 819, 824 |
| MaeII | ACGT | 1 cut(s) | 145 |
| MaeIII | GTNAC | 3 cut(s) | 403, 835, 923 |
| MalI | GATC | 4 cut(s) | 47, 531, 769, 808 |
| MboI | GATC | 4 cut(s) | 45, 529, 767, 806 |
| MboII | GAAGA | 8 cut(s) | 60, 185, 210, 296, 539, 698, 901, 929 |
| MflI | RGATCY | 2 cut(s) | 767, 806 |
| MhlI | GDGCHC | 1 cut(s) | 715 |
| MluCI | AATT | 6 cut(s) | 248, 263, 287, 453, 476, 607 |
| MlyI | GAGTC | 1 cut(s) | 585 |
| MnlI | CCTC | 5 cut(s) | 59, 67, 108, 381, 535 |
| Mph1103I | ATGCAT | 1 cut(s) | 103 |
| MseI | TTAA | 1 cut(s) | 720 |
| MslI | CAYNNNNRTG | 3 cut(s) | 687, 932, 977 |
| MspA1I | CMGCKG | 1 cut(s) | 842 |
| MspCI | CTTAAG | 1 cut(s) | 719 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 440, 662, 758, 983 |
| NcoI | CCATGG | 2 cut(s) | 829, 978 |
| NdeII | GATC | 4 cut(s) | 45, 529, 767, 806 |
| NheI | GCTAGC | 1 cut(s) | 362 |
| NlaIII | CATG | 7 cut(s) | 163, 323, 590, 639, 833, 900, 982 |
| NlaIV | GGNNCC | 2 cut(s) | 769, 808 |
| NsiI | ATGCAT | 1 cut(s) | 103 |
| NspI | RCATGY | 1 cut(s) | 639 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 142 |
| PfeI | GAWTC | 2 cut(s) | 215, 259 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 620 |
| PkrI | GCNGC | 2 cut(s) | 442, 667 |
| PleI | GAGTC | 1 cut(s) | 585 |
| PpsI | GAGTC | 1 cut(s) | 585 |
| PspFI | CCCAGC | 1 cut(s) | 55 |
| PspN4I | GGNNCC | 2 cut(s) | 769, 808 |
| PspPI | GGNCC | 1 cut(s) | 418 |
| PsuI | RGATCY | 2 cut(s) | 767, 806 |
| RsaI | GTAC | 4 cut(s) | 119, 598, 634, 878 |
| RsaNI | GTAC | 4 cut(s) | 118, 597, 633, 877 |
| RseI | CAYNNNNRTG | 3 cut(s) | 687, 932, 977 |
| SaqAI | TTAA | 1 cut(s) | 720 |
| SatI | GCNGC | 2 cut(s) | 441, 666 |
| Sau3AI | GATC | 4 cut(s) | 45, 529, 767, 806 |
| Sau96I | GGNCC | 1 cut(s) | 418 |
| ScaI | AGTACT | 1 cut(s) | 878 |
| SchI | GAGTC | 1 cut(s) | 585 |
| SduI | GDGCHC | 1 cut(s) | 715 |
| SfaNI | GCATC | 5 cut(s) | 88, 110, 550, 748, 924 |
| SinI | GGWCC | 1 cut(s) | 418 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 687, 932, 977 |
| SmlI | CTYRAG | 2 cut(s) | 719, 787 |
| SmoI | CTYRAG | 2 cut(s) | 719, 787 |
| Sse9I | AATT | 6 cut(s) | 248, 263, 287, 453, 476, 607 |
| SsiI | CCGC | 2 cut(s) | 297, 840 |
| SspMI | CTAG | 5 cut(s) | 363, 503, 762, 819, 824 |
| StyI | CCWWGG | 3 cut(s) | 421, 829, 978 |
| TaaI | ACNGT | 2 cut(s) | 29, 512 |
| TaiI | ACGT | 1 cut(s) | 148 |
| TasI | AATT | 6 cut(s) | 248, 263, 287, 453, 476, 607 |
| TatI | WGTACW | 1 cut(s) | 876 |
| TfiI | GAWTC | 2 cut(s) | 215, 259 |
| Tru1I | TTAA | 1 cut(s) | 720 |
| Tru9I | TTAA | 1 cut(s) | 720 |
| TscAI | CASTG | 2 cut(s) | 625, 1003 |
| TseI | GCWGC | 2 cut(s) | 440, 665 |
| TspDTI | ATGAA | 4 cut(s) | 176, 297, 369, 562 |
| TspGWI | ACGGA | 1 cut(s) | 461 |
| TspRI | CASTG | 2 cut(s) | 625, 1003 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 620 |
| Vha464I | CTTAAG | 1 cut(s) | 719 |
| VneI | GTGCAC | 1 cut(s) | 711 |
| VpaK11BI | GGWCC | 1 cut(s) | 418 |
| XapI | RAATTY | 1 cut(s) | 287 |
| XceI | RCATGY | 1 cut(s) | 639 |
| XmiI | GTMKAC | 1 cut(s) | 150 |
| XspI | CTAG | 5 cut(s) | 363, 503, 762, 819, 824 |
| ZrmI | AGTACT | 1 cut(s) | 878 |
| Zsp2I | ATGCAT | 1 cut(s) | 103 |