Belongs to the peptidase C1 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 609 |
| AccII | CGCG | 1 cut(s) | 638 |
| AciI | CCGC | 2 cut(s) | 10, 636 |
| AclWI | GGATC | 3 cut(s) | 326, 627, 640 |
| AcsI | RAATTY | 1 cut(s) | 161 |
| AcuI | CTGAAG | 3 cut(s) | 26, 294, 486 |
| AfaI | GTAC | 2 cut(s) | 134, 143 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 484, 485, 543 |
| AgsI | TTSAA | 1 cut(s) | 88 |
| AjnI | CCWGG | 1 cut(s) | 483 |
| AluBI | AGCT | 6 cut(s) | 29, 113, 251, 308, 328, 342 |
| AluI | AGCT | 6 cut(s) | 29, 113, 251, 308, 328, 342 |
| Alw26I | GTCTC | 1 cut(s) | 162 |
| AlwI | GGATC | 3 cut(s) | 326, 627, 640 |
| AoxI | GGCC | 1 cut(s) | 454 |
| ApeKI | GCWGC | 3 cut(s) | 305, 530, 583 |
| ApoI | RAATTY | 1 cut(s) | 161 |
| AspS9I | GGNCC | 1 cut(s) | 454 |
| BaeGI | GKGCMC | 1 cut(s) | 549 |
| BamHI | GGATCC | 1 cut(s) | 632 |
| BauI | CACGAG | 2 cut(s) | 14, 548 |
| BbvI | GCAGC | 3 cut(s) | 317, 542, 570 |
| BccI | CCATC | 3 cut(s) | 121, 545, 626 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 557, 591 |
| BciT130I | CCWGG | 1 cut(s) | 485 |
| BcoDI | GTCTC | 1 cut(s) | 162 |
| BfaI | CTAG | 1 cut(s) | 353 |
| BfmI | CTRYAG | 1 cut(s) | 584 |
| BfuAI | ACCTGC | 1 cut(s) | 609 |
| BisI | GCNGC | 4 cut(s) | 11, 306, 531, 584 |
| BlsI | GCNGC | 4 cut(s) | 12, 307, 532, 585 |
| Bme1390I | CCNGG | 1 cut(s) | 485 |
| BmgT120I | GGNCC | 1 cut(s) | 454 |
| BmiI | GGNNCC | 1 cut(s) | 634 |
| BmrFI | CCNGG | 1 cut(s) | 485 |
| BmrI | ACTGGG | 1 cut(s) | 552 |
| BmsI | GCATC | 2 cut(s) | 43, 613 |
| BmuI | ACTGGG | 1 cut(s) | 552 |
| BpmI | CTGGAG | 1 cut(s) | 405 |
| Bsa29I | ATCGAT | 1 cut(s) | 621 |
| BsaI | GGTCTC | 1 cut(s) | 162 |
| BsaJI | CCNNGG | 1 cut(s) | 484 |
| BsaWI | WCCGGW | 1 cut(s) | 335 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 484, 485, 543 |
| Bse1I | ACTGG | 2 cut(s) | 388, 547 |
| Bse3DI | GCAATG | 1 cut(s) | 201 |
| BseBI | CCWGG | 1 cut(s) | 485 |
| BseCI | ATCGAT | 1 cut(s) | 621 |
| BseDI | CCNNGG | 1 cut(s) | 484 |
| BseGI | GGATG | 4 cut(s) | 58, 438, 521, 559 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 484, 485, 543 |
| BseMI | GCAATG | 1 cut(s) | 201 |
| BseMII | CTCAG | 2 cut(s) | 140, 524 |
| BseNI | ACTGG | 2 cut(s) | 388, 547 |
| BseRI | GAGGAG | 2 cut(s) | 32, 197 |
| BseSI | GKGCMC | 1 cut(s) | 549 |
| BseXI | GCAGC | 3 cut(s) | 317, 542, 570 |
| Bsh1236I | CGCG | 1 cut(s) | 638 |
| BshFI | GGCC | 1 cut(s) | 456 |
| BshVI | ATCGAT | 1 cut(s) | 621 |
| BsiSI | CCGG | 1 cut(s) | 336 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 484, 485, 543 |
| BsmAI | GTCTC | 1 cut(s) | 162 |
| BsmI | GAATGC | 2 cut(s) | 520, 580 |
| BsnI | GGCC | 1 cut(s) | 456 |
| Bso31I | GGTCTC | 1 cut(s) | 162 |
| Bsp1286I | GDGCHC | 1 cut(s) | 549 |
| Bsp143I | GATC | 5 cut(s) | 81, 90, 318, 394, 632 |
| BspACI | CCGC | 2 cut(s) | 10, 636 |
| BspANI | GGCC | 1 cut(s) | 456 |
| BspCNI | CTCAG | 2 cut(s) | 141, 523 |
| BspDI | ATCGAT | 1 cut(s) | 621 |
| BspFNI | CGCG | 1 cut(s) | 638 |
| BspLI | GGNNCC | 1 cut(s) | 634 |
| BspMAI | CTGCAG | 1 cut(s) | 588 |
| BspMI | ACCTGC | 1 cut(s) | 609 |
| BspPI | GGATC | 3 cut(s) | 326, 627, 640 |
| BspTNI | GGTCTC | 1 cut(s) | 162 |
| BsrDI | GCAATG | 1 cut(s) | 201 |
| BsrI | ACTGG | 2 cut(s) | 388, 547 |
| BssECI | CCNNGG | 1 cut(s) | 484 |
| BssMI | GATC | 5 cut(s) | 81, 90, 318, 394, 632 |
| BssSI | CACGAG | 2 cut(s) | 14, 548 |
| Bst2BI | CACGAG | 2 cut(s) | 14, 548 |
| Bst2UI | CCWGG | 1 cut(s) | 485 |
| Bst4CI | ACNGT | 1 cut(s) | 401 |
| Bst6I | CTCTTC | 1 cut(s) | 54 |
| BstC8I | GCNNGC | 1 cut(s) | 588 |
| BstDEI | CTNAG | 2 cut(s) | 149, 510 |
| BstF5I | GGATG | 4 cut(s) | 58, 438, 521, 559 |
| BstFNI | CGCG | 1 cut(s) | 638 |
| BstKTI | GATC | 5 cut(s) | 84, 93, 321, 397, 635 |
| BstMAI | GTCTC | 1 cut(s) | 162 |
| BstMBI | GATC | 5 cut(s) | 81, 90, 318, 394, 632 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 10, 296, 305, 453, 623 |
| BstNI | CCWGG | 1 cut(s) | 485 |
| BstSCI | CCNGG | 1 cut(s) | 483 |
| BstSFI | CTRYAG | 1 cut(s) | 584 |
| BstSLI | GKGCMC | 1 cut(s) | 549 |
| BstUI | CGCG | 1 cut(s) | 638 |
| BstV1I | GCAGC | 3 cut(s) | 317, 542, 570 |
| BstX2I | RGATCY | 1 cut(s) | 632 |
| BstYI | RGATCY | 1 cut(s) | 632 |
| Bsu15I | ATCGAT | 1 cut(s) | 621 |
| BsuRI | GGCC | 1 cut(s) | 456 |
| BsuTUI | ATCGAT | 1 cut(s) | 621 |
| BtsCI | GGATG | 4 cut(s) | 58, 438, 521, 559 |
| BtsIMutI | CAGTG | 3 cut(s) | 146, 381, 540 |
| BveI | ACCTGC | 1 cut(s) | 609 |
| Cac8I | GCNNGC | 1 cut(s) | 588 |
| Cfr13I | GGNCC | 1 cut(s) | 454 |
| ClaI | ATCGAT | 1 cut(s) | 621 |
| Csp6I | GTAC | 2 cut(s) | 133, 142 |
| CviAII | CATG | 1 cut(s) | 35 |
| CviQI | GTAC | 2 cut(s) | 133, 142 |
| DdeI | CTNAG | 2 cut(s) | 149, 510 |
| DpnI | GATC | 5 cut(s) | 83, 92, 320, 396, 634 |
| DpnII | GATC | 5 cut(s) | 81, 90, 318, 394, 632 |
| Eam1104I | CTCTTC | 1 cut(s) | 54 |
| EarI | CTCTTC | 1 cut(s) | 54 |
| Eco31I | GGTCTC | 1 cut(s) | 162 |
| Eco57I | CTGAAG | 3 cut(s) | 26, 294, 486 |
| EcoO109I | RGGNCCY | 1 cut(s) | 454 |
| EcoRII | CCWGG | 1 cut(s) | 483 |
| FaeI | CATG | 1 cut(s) | 38 |
| FaiI | YATR | 3 cut(s) | 36, 107, 267 |
| FatI | CATG | 1 cut(s) | 34 |
| Fnu4HI | GCNGC | 4 cut(s) | 11, 306, 531, 584 |
| FokI | GGATG | 4 cut(s) | 65, 425, 508, 566 |
| Fsp4HI | GCNGC | 4 cut(s) | 11, 306, 531, 584 |
| FspBI | CTAG | 1 cut(s) | 353 |
| GluI | GCNGC | 4 cut(s) | 11, 306, 531, 584 |
| GsuI | CTGGAG | 1 cut(s) | 405 |
| HaeIII | GGCC | 1 cut(s) | 456 |
| HapII | CCGG | 1 cut(s) | 336 |
| Hin1II | CATG | 1 cut(s) | 38 |
| HincII | GTYRAC | 1 cut(s) | 493 |
| HindII | GTYRAC | 1 cut(s) | 493 |
| HindIII | AAGCTT | 2 cut(s) | 111, 340 |
| HinfI | GANTC | 1 cut(s) | 20 |
| HpaII | CCGG | 1 cut(s) | 336 |
| Hpy166II | GTNNAC | 3 cut(s) | 46, 144, 493 |
| Hpy188I | TCNGA | 2 cut(s) | 313, 513 |
| Hpy188III | TCNNGA | 1 cut(s) | 88 |
| Hpy8I | GTNNAC | 3 cut(s) | 46, 144, 493 |
| Hpy99I | CGWCG | 2 cut(s) | 101, 572 |
| HpyAV | CCTTC | 3 cut(s) | 58, 308, 439 |
| HpyCH4III | ACNGT | 1 cut(s) | 401 |
| HpyCH4IV | ACGT | 1 cut(s) | 96 |
| HpyCH4V | TGCA | 5 cut(s) | 56, 305, 504, 586, 626 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 10, 296, 305, 453, 623 |
| HpyF3I | CTNAG | 2 cut(s) | 149, 510 |
| HpySE526I | ACGT | 1 cut(s) | 96 |
| Hsp92II | CATG | 1 cut(s) | 38 |
| Kzo9I | GATC | 5 cut(s) | 81, 90, 318, 394, 632 |
| Lsp1109I | GCAGC | 3 cut(s) | 317, 542, 570 |
| LweI | GCATC | 2 cut(s) | 43, 613 |
| MaeI | CTAG | 1 cut(s) | 353 |
| MaeII | ACGT | 1 cut(s) | 96 |
| MaeIII | GTNAC | 1 cut(s) | 377 |
| MalI | GATC | 5 cut(s) | 83, 92, 320, 396, 634 |
| MboI | GATC | 5 cut(s) | 81, 90, 318, 394, 632 |
| MboII | GAAGA | 2 cut(s) | 71, 76 |
| MflI | RGATCY | 1 cut(s) | 632 |
| MhlI | GDGCHC | 1 cut(s) | 549 |
| MluCI | AATT | 4 cut(s) | 161, 345, 434, 472 |
| MlyI | GAGTC | 1 cut(s) | 29 |
| MmeI | TCCRAC | 1 cut(s) | 261 |
| MnlI | CCTC | 7 cut(s) | 10, 144, 175, 360, 380, 544, 544 |
| MseI | TTAA | 4 cut(s) | 117, 155, 330, 344 |
| MslI | CAYNNNNRTG | 1 cut(s) | 552 |
| MspI | CCGG | 1 cut(s) | 336 |
| MspR9I | CCNGG | 1 cut(s) | 485 |
| Mva1269I | GAATGC | 2 cut(s) | 520, 580 |
| MvaI | CCWGG | 1 cut(s) | 485 |
| MvnI | CGCG | 1 cut(s) | 638 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 10, 296, 305, 453, 623 |
| NdeII | GATC | 5 cut(s) | 81, 90, 318, 394, 632 |
| NlaIII | CATG | 1 cut(s) | 38 |
| NlaIV | GGNNCC | 1 cut(s) | 634 |
| NmuCI | GTSAC | 1 cut(s) | 377 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 37, 196 |
| PctI | GAATGC | 2 cut(s) | 520, 580 |
| PkrI | GCNGC | 4 cut(s) | 12, 307, 532, 585 |
| PleI | GAGTC | 1 cut(s) | 28 |
| PpsI | GAGTC | 1 cut(s) | 28 |
| Psp6I | CCWGG | 1 cut(s) | 483 |
| PspGI | CCWGG | 1 cut(s) | 483 |
| PspN4I | GGNNCC | 1 cut(s) | 634 |
| PspPI | GGNCC | 1 cut(s) | 454 |
| PstI | CTGCAG | 1 cut(s) | 588 |
| PsuI | RGATCY | 1 cut(s) | 632 |
| RsaI | GTAC | 2 cut(s) | 134, 143 |
| RsaNI | GTAC | 2 cut(s) | 133, 142 |
| RseI | CAYNNNNRTG | 1 cut(s) | 552 |
| SaqAI | TTAA | 4 cut(s) | 117, 155, 330, 344 |
| SatI | GCNGC | 4 cut(s) | 11, 306, 531, 584 |
| Sau3AI | GATC | 5 cut(s) | 81, 90, 318, 394, 632 |
| Sau96I | GGNCC | 1 cut(s) | 454 |
| SchI | GAGTC | 1 cut(s) | 29 |
| ScrFI | CCNGG | 1 cut(s) | 485 |
| SduI | GDGCHC | 1 cut(s) | 549 |
| SfaNI | GCATC | 2 cut(s) | 43, 613 |
| SfcI | CTRYAG | 1 cut(s) | 584 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 552 |
| Sse9I | AATT | 4 cut(s) | 161, 345, 434, 472 |
| SsiI | CCGC | 2 cut(s) | 10, 636 |
| SspI | AATATT | 1 cut(s) | 227 |
| SspMI | CTAG | 1 cut(s) | 353 |
| StyD4I | CCNGG | 1 cut(s) | 483 |
| TaaI | ACNGT | 1 cut(s) | 401 |
| TaiI | ACGT | 1 cut(s) | 99 |
| TaqI | TCGA | 4 cut(s) | 31, 99, 190, 621 |
| TasI | AATT | 4 cut(s) | 161, 345, 434, 472 |
| TatI | WGTACW | 1 cut(s) | 141 |
| TauI | GCSGC | 1 cut(s) | 13 |
| Tru1I | TTAA | 4 cut(s) | 117, 155, 330, 344 |
| Tru9I | TTAA | 4 cut(s) | 117, 155, 330, 344 |
| TscAI | CASTG | 3 cut(s) | 153, 388, 547 |
| TseFI | GTSAC | 1 cut(s) | 377 |
| TseI | GCWGC | 3 cut(s) | 305, 530, 583 |
| Tsp45I | GTSAC | 1 cut(s) | 377 |
| TspDTI | ATGAA | 2 cut(s) | 94, 427 |
| TspRI | CASTG | 3 cut(s) | 153, 388, 547 |
| XapI | RAATTY | 1 cut(s) | 161 |
| XspI | CTAG | 1 cut(s) | 353 |