Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2782 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 40 |
| AatII | GACGTC | 2 cut(s) | 548, 1562 |
| Acc36I | ACCTGC | 1 cut(s) | 2782 |
| AccB1I | GGYRCC | 2 cut(s) | 445, 1753 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 611 |
| AccI | GTMKAC | 2 cut(s) | 2494, 2659 |
| AccII | CGCG | 5 cut(s) | 27, 49, 64, 1263, 1701 |
| AciI | CCGC | 9 cut(s) | 27, 47, 64, 449, 554, 707, 1261, 3032, 3202 |
| AclWI | GGATC | 3 cut(s) | 149, 2162, 2205 |
| AcoI | YGGCCR | 1 cut(s) | 357 |
| AcsI | RAATTY | 9 cut(s) | 1040, 1345, 1738, 1791, 1845, 2009, 2713, 2743, 2893 |
| AcuI | CTGAAG | 4 cut(s) | 1134, 1293, 2412, 2976 |
| AcvI | CACGTG | 1 cut(s) | 2924 |
| AcyI | GRCGYC | 3 cut(s) | 446, 545, 1559 |
| AdeI | CACNNNGTG | 2 cut(s) | 1553, 1613 |
| AfaI | GTAC | 5 cut(s) | 161, 209, 267, 2242, 2359 |
| AfeI | AGCGCT | 1 cut(s) | 2905 |
| AflII | CTTAAG | 1 cut(s) | 323 |
| AflIII | ACRYGT | 3 cut(s) | 2921, 3017, 3206 |
| AjiI | CACGTC | 1 cut(s) | 3209 |
| AleI | CACNNNNGTG | 1 cut(s) | 2229 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1936, 1968 |
| Alw21I | GWGCWC | 4 cut(s) | 314, 366, 1609, 2505 |
| Alw26I | GTCTC | 6 cut(s) | 26, 180, 633, 776, 1368, 1550 |
| Alw44I | GTGCAC | 1 cut(s) | 1605 |
| AlwI | GGATC | 3 cut(s) | 149, 2162, 2205 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 532, 1340, 2059, 2171 |
| Ama87I | CYCGRG | 2 cut(s) | 482, 1836 |
| Aor51HI | AGCGCT | 1 cut(s) | 2905 |
| AoxI | GGCC | 3 cut(s) | 357, 458, 2367 |
| ApaLI | GTGCAC | 1 cut(s) | 1605 |
| ApoI | RAATTY | 9 cut(s) | 1040, 1345, 1738, 1791, 1845, 2009, 2713, 2743, 2893 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 1028, 1349, 1728, 2622, 2961 |
| AspLEI | GCGC | 3 cut(s) | 448, 2463, 2906 |
| AspS9I | GGNCC | 4 cut(s) | 66, 668, 2204, 3095 |
| AsuC2I | CCSGG | 4 cut(s) | 178, 483, 484, 666 |
| AvaI | CYCGRG | 2 cut(s) | 482, 1836 |
| AvaII | GGWCC | 4 cut(s) | 66, 668, 2204, 3095 |
| AxyI | CCTNAGG | 1 cut(s) | 2364 |
| BaeGI | GKGCMC | 1 cut(s) | 1609 |
| BanI | GGYRCC | 2 cut(s) | 445, 1753 |
| BanII | GRGCYC | 1 cut(s) | 366 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1742, 1774 |
| BauI | CACGAG | 1 cut(s) | 2124 |
| BbrPI | CACGTG | 1 cut(s) | 2924 |
| BbsI | GAAGAC | 2 cut(s) | 540, 2418 |
| Bbv12I | GWGCWC | 4 cut(s) | 314, 366, 1609, 2505 |
| BceAI | ACGGC | 2 cut(s) | 131, 2649 |
| BciVI | GTATCC | 3 cut(s) | 3, 393, 1804 |
| BcnI | CCSGG | 4 cut(s) | 178, 483, 484, 666 |
| BcoDI | GTCTC | 6 cut(s) | 26, 180, 633, 776, 1368, 1550 |
| BfaI | CTAG | 5 cut(s) | 474, 915, 1743, 2049, 2676 |
| BfmI | CTRYAG | 3 cut(s) | 2250, 2271, 2789 |
| BfoI | RGCGCY | 2 cut(s) | 449, 2907 |
| BfrI | CTTAAG | 1 cut(s) | 323 |
| BfuAI | ACCTGC | 1 cut(s) | 2782 |
| BfuI | GTATCC | 3 cut(s) | 3, 393, 1804 |
| Bme18I | GGWCC | 4 cut(s) | 66, 668, 2204, 3095 |
| BmeT110I | CYCGRG | 2 cut(s) | 482, 1836 |
| BmgBI | CACGTC | 1 cut(s) | 3209 |
| BmgT120I | GGNCC | 4 cut(s) | 66, 668, 2204, 3095 |
| BmiI | GGNNCC | 4 cut(s) | 447, 669, 1755, 3011 |
| BmrI | ACTGGG | 1 cut(s) | 23 |
| BmsI | GCATC | 9 cut(s) | 673, 1276, 1505, 1855, 2120, 2323, 2365, 2941, 3184 |
| BmuI | ACTGGG | 1 cut(s) | 23 |
| BpiI | GAAGAC | 2 cut(s) | 540, 2418 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 44, 76, 177, 209, 710, 742 |
| Bpu10I | CCTNAGC | 1 cut(s) | 758 |
| BpuEI | CTTGAG | 2 cut(s) | 320, 2954 |
| BpuMI | CCSGG | 4 cut(s) | 178, 483, 484, 666 |
| BsaAI | YACGTR | 1 cut(s) | 2924 |
| BsaBI | GATNNNNATC | 1 cut(s) | 3222 |
| BsaHI | GRCGYC | 3 cut(s) | 446, 545, 1559 |
| BsaI | GGTCTC | 1 cut(s) | 1368 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2027, 2057 |
| Bse1I | ACTGG | 1 cut(s) | 18 |
| Bse21I | CCTNAGG | 1 cut(s) | 2364 |
| Bse3DI | GCAATG | 5 cut(s) | 218, 686, 1641, 1986, 3158 |
| Bse8I | GATNNNNATC | 1 cut(s) | 3222 |
| BseJI | GATNNNNATC | 1 cut(s) | 3222 |
| BseMI | GCAATG | 5 cut(s) | 218, 686, 1641, 1986, 3158 |
| BseMII | CTCAG | 7 cut(s) | 476, 575, 720, 1101, 1185, 1332, 3125 |
| BseNI | ACTGG | 1 cut(s) | 18 |
| BseRI | GAGGAG | 2 cut(s) | 1680, 1854 |
| BseSI | GKGCMC | 1 cut(s) | 1609 |
| BseYI | CCCAGC | 2 cut(s) | 275, 2552 |
| BsgI | GTGCAG | 2 cut(s) | 1562, 2534 |
| Bsh1236I | CGCG | 5 cut(s) | 27, 49, 64, 1263, 1701 |
| BshFI | GGCC | 3 cut(s) | 359, 460, 2369 |
| BshNI | GGYRCC | 2 cut(s) | 445, 1753 |
| BsiHKAI | GWGCWC | 4 cut(s) | 314, 366, 1609, 2505 |
| BsiHKCI | CYCGRG | 2 cut(s) | 482, 1836 |
| BsiSI | CCGG | 4 cut(s) | 87, 177, 483, 665 |
| BslFI | GGGAC | 5 cut(s) | 30, 304, 776, 908, 2468 |
| BsmAI | GTCTC | 6 cut(s) | 26, 180, 633, 776, 1368, 1550 |
| BsmBI | CGTCTC | 3 cut(s) | 26, 776, 1550 |
| BsmFI | GGGAC | 5 cut(s) | 30, 304, 776, 908, 2468 |
| BsmI | GAATGC | 2 cut(s) | 1228, 3000 |
| BsnI | GGCC | 3 cut(s) | 359, 460, 2369 |
| Bso31I | GGTCTC | 1 cut(s) | 1368 |
| BsoBI | CYCGRG | 2 cut(s) | 482, 1836 |
| Bsp1286I | GDGCHC | 4 cut(s) | 314, 366, 1609, 2505 |
| Bsp1407I | TGTACA | 2 cut(s) | 159, 207 |
| Bsp143I | GATC | 7 cut(s) | 54, 141, 1369, 1594, 1629, 2167, 2197 |
| BspACI | CCGC | 9 cut(s) | 27, 47, 64, 449, 554, 707, 1261, 3032, 3202 |
| BspANI | GGCC | 3 cut(s) | 359, 460, 2369 |
| BspCNI | CTCAG | 7 cut(s) | 475, 574, 721, 1100, 1186, 1333, 3126 |
| BspFNI | CGCG | 5 cut(s) | 27, 49, 64, 1263, 1701 |
| BspLI | GGNNCC | 4 cut(s) | 447, 669, 1755, 3011 |
| BspMAI | CTGCAG | 2 cut(s) | 2275, 2793 |
| BspMI | ACCTGC | 1 cut(s) | 2782 |
| BspPI | GGATC | 3 cut(s) | 149, 2162, 2205 |
| BspQI | GCTCTTC | 2 cut(s) | 1218, 3141 |
| BspT107I | GGYRCC | 2 cut(s) | 445, 1753 |
| BspTI | CTTAAG | 1 cut(s) | 323 |
| BspTNI | GGTCTC | 1 cut(s) | 1368 |
| BsrDI | GCAATG | 5 cut(s) | 218, 686, 1641, 1986, 3158 |
| BsrGI | TGTACA | 2 cut(s) | 159, 207 |
| BsrI | ACTGG | 1 cut(s) | 18 |
| BssMI | GATC | 7 cut(s) | 54, 141, 1369, 1594, 1629, 2167, 2197 |
| BssNI | GRCGYC | 3 cut(s) | 446, 545, 1559 |
| BssSI | CACGAG | 1 cut(s) | 2124 |
| BssT1I | CCWWGG | 2 cut(s) | 1647, 3162 |
| Bst2BI | CACGAG | 1 cut(s) | 2124 |
| Bst6I | CTCTTC | 3 cut(s) | 1218, 2471, 3141 |
| BstACI | GRCGYC | 3 cut(s) | 446, 545, 1559 |
| BstAFI | CTTAAG | 1 cut(s) | 323 |
| BstAUI | TGTACA | 2 cut(s) | 159, 207 |
| BstBAI | YACGTR | 1 cut(s) | 2924 |
| BstC8I | GCNNGC | 6 cut(s) | 357, 558, 1265, 2371, 2681, 3030 |
| BstDSI | CCRYGG | 1 cut(s) | 3098 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 588, 2307, 2789 |
| BstFNI | CGCG | 5 cut(s) | 27, 49, 64, 1263, 1701 |
| BstH2I | RGCGCY | 2 cut(s) | 449, 2907 |
| BstHHI | GCGC | 3 cut(s) | 448, 2463, 2906 |
| BstKTI | GATC | 7 cut(s) | 57, 144, 1372, 1597, 1632, 2170, 2200 |
| BstMAI | GTCTC | 6 cut(s) | 26, 180, 633, 776, 1368, 1550 |
| BstMBI | GATC | 7 cut(s) | 54, 141, 1369, 1594, 1629, 2167, 2197 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 361, 457, 2273, 2375 |
| BstNSI | RCATGY | 2 cut(s) | 3021, 3032 |
| BstSFI | CTRYAG | 3 cut(s) | 2250, 2271, 2789 |
| BstSLI | GKGCMC | 1 cut(s) | 1609 |
| BstUI | CGCG | 5 cut(s) | 27, 49, 64, 1263, 1701 |
| BstV2I | GAAGAC | 2 cut(s) | 540, 2418 |
| BstX2I | RGATCY | 1 cut(s) | 2167 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1890, 2552 |
| BstYI | RGATCY | 1 cut(s) | 2167 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2364 |
| BsuI | GTATCC | 3 cut(s) | 3, 393, 1804 |
| BsuRI | GGCC | 3 cut(s) | 359, 460, 2369 |
| BtgI | CCRYGG | 1 cut(s) | 3098 |
| BtgZI | GCGATG | 1 cut(s) | 2388 |
| BtrI | CACGTC | 1 cut(s) | 3209 |
| BtsIMutI | CAGTG | 4 cut(s) | 604, 996, 1338, 2250 |
| BveI | ACCTGC | 1 cut(s) | 2782 |
| Cac8I | GCNNGC | 6 cut(s) | 357, 558, 1265, 2371, 2681, 3030 |
| CaiI | CAGNNNCTG | 4 cut(s) | 532, 1340, 2059, 2171 |
| CfoI | GCGC | 3 cut(s) | 448, 2463, 2906 |
| Cfr13I | GGNCC | 4 cut(s) | 66, 668, 2204, 3095 |
| Cfr9I | CCCGGG | 1 cut(s) | 482 |
| CsiI | ACCWGGT | 2 cut(s) | 495, 2446 |
| Csp6I | GTAC | 5 cut(s) | 160, 208, 266, 2241, 2358 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2226, 2261 |
| CviAII | CATG | 7 cut(s) | 167, 1523, 1964, 2866, 3018, 3029, 3081 |
| CviQI | GTAC | 5 cut(s) | 160, 208, 266, 2241, 2358 |
| DinI | GGCGCC | 1 cut(s) | 447 |
| DpnI | GATC | 7 cut(s) | 56, 143, 1371, 1596, 1631, 2169, 2199 |
| DpnII | GATC | 7 cut(s) | 54, 141, 1369, 1594, 1629, 2167, 2197 |
| DraI | TTTAAA | 3 cut(s) | 2728, 2989, 3088 |
| DraIII | CACNNNGTG | 2 cut(s) | 1553, 1613 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 40 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 357 |
| Eam1104I | CTCTTC | 3 cut(s) | 1218, 2471, 3141 |
| EarI | CTCTTC | 3 cut(s) | 1218, 2471, 3141 |
| EciI | GGCGGA | 2 cut(s) | 722, 3217 |
| Ecl136II | GAGCTC | 1 cut(s) | 364 |
| Eco130I | CCWWGG | 2 cut(s) | 1647, 3162 |
| Eco24I | GRGCYC | 1 cut(s) | 366 |
| Eco31I | GGTCTC | 1 cut(s) | 1368 |
| Eco47I | GGWCC | 4 cut(s) | 66, 668, 2204, 3095 |
| Eco47III | AGCGCT | 1 cut(s) | 2905 |
| Eco53kI | GAGCTC | 1 cut(s) | 364 |
| Eco57I | CTGAAG | 4 cut(s) | 1134, 1293, 2412, 2976 |
| Eco72I | CACGTG | 1 cut(s) | 2924 |
| Eco81I | CCTNAGG | 1 cut(s) | 2364 |
| Eco88I | CYCGRG | 2 cut(s) | 482, 1836 |
| EcoICRI | GAGCTC | 1 cut(s) | 364 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 588, 2307, 2789 |
| EcoRI | GAATTC | 2 cut(s) | 1738, 1845 |
| EcoT14I | CCWWGG | 2 cut(s) | 1647, 3162 |
| EcoT22I | ATGCAT | 1 cut(s) | 688 |
| EcoT38I | GRGCYC | 1 cut(s) | 366 |
| EgeI | GGCGCC | 1 cut(s) | 447 |
| EheI | GGCGCC | 1 cut(s) | 447 |
| ErhI | CCWWGG | 2 cut(s) | 1647, 3162 |
| Esp3I | CGTCTC | 3 cut(s) | 26, 776, 1550 |
| FaeI | CATG | 7 cut(s) | 170, 1526, 1967, 2869, 3021, 3032, 3084 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1716, 1748, 2541, 2573, 2997, 3029 |
| FaqI | GGGAC | 5 cut(s) | 30, 304, 776, 908, 2468 |
| FatI | CATG | 7 cut(s) | 166, 1522, 1963, 2865, 3017, 3028, 3080 |
| FauI | CCCGC | 1 cut(s) | 20 |
| FblI | GTMKAC | 2 cut(s) | 2494, 2659 |
| FriOI | GRGCYC | 1 cut(s) | 366 |
| FspBI | CTAG | 5 cut(s) | 474, 915, 1743, 2049, 2676 |
| GlaI | GCGC | 3 cut(s) | 447, 2462, 2905 |
| GsaI | CCCAGC | 2 cut(s) | 279, 2556 |
| HaeII | RGCGCY | 2 cut(s) | 449, 2907 |
| HaeIII | GGCC | 3 cut(s) | 359, 460, 2369 |
| HapII | CCGG | 4 cut(s) | 87, 177, 483, 665 |
| HhaI | GCGC | 3 cut(s) | 448, 2463, 2906 |
| Hin1I | GRCGYC | 3 cut(s) | 446, 545, 1559 |
| Hin1II | CATG | 7 cut(s) | 170, 1526, 1967, 2869, 3021, 3032, 3084 |
| Hin6I | GCGC | 3 cut(s) | 446, 2461, 2904 |
| HinP1I | GCGC | 3 cut(s) | 446, 2461, 2904 |
| HincII | GTYRAC | 1 cut(s) | 2919 |
| HindII | GTYRAC | 1 cut(s) | 2919 |
| HpaII | CCGG | 4 cut(s) | 87, 177, 483, 665 |
| Hpy99I | CGWCG | 3 cut(s) | 1564, 2217, 2232 |
| HpyCH4IV | ACGT | 5 cut(s) | 545, 1559, 2923, 3037, 3208 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 361, 457, 2273, 2375 |
| HpySE526I | ACGT | 5 cut(s) | 545, 1559, 2923, 3037, 3208 |
| Hsp92I | GRCGYC | 3 cut(s) | 446, 545, 1559 |
| Hsp92II | CATG | 7 cut(s) | 170, 1526, 1967, 2869, 3021, 3032, 3084 |
| HspAI | GCGC | 3 cut(s) | 446, 2461, 2904 |
| KasI | GGCGCC | 1 cut(s) | 445 |
| Kzo9I | GATC | 7 cut(s) | 54, 141, 1369, 1594, 1629, 2167, 2197 |
| LguI | GCTCTTC | 2 cut(s) | 1218, 3141 |
| LmnI | GCTCC | 2 cut(s) | 2500, 2911 |
| LweI | GCATC | 9 cut(s) | 673, 1276, 1505, 1855, 2120, 2323, 2365, 2941, 3184 |
| MabI | ACCWGGT | 2 cut(s) | 495, 2446 |
| MaeI | CTAG | 5 cut(s) | 474, 915, 1743, 2049, 2676 |
| MaeII | ACGT | 5 cut(s) | 545, 1559, 2923, 3037, 3208 |
| MaeIII | GTNAC | 6 cut(s) | 1545, 1617, 1983, 2014, 2442, 2590 |
| MalI | GATC | 7 cut(s) | 56, 143, 1371, 1596, 1631, 2169, 2199 |
| MboI | GATC | 7 cut(s) | 54, 141, 1369, 1594, 1629, 2167, 2197 |
| MfeI | CAATTG | 2 cut(s) | 1587, 1968 |
| MflI | RGATCY | 1 cut(s) | 2167 |
| MhlI | GDGCHC | 4 cut(s) | 314, 366, 1609, 2505 |
| Mly113I | GGCGCC | 1 cut(s) | 446 |
| MmeI | TCCRAC | 2 cut(s) | 2744, 3002 |
| Mph1103I | ATGCAT | 1 cut(s) | 688 |
| MroXI | GAANNNNTTC | 5 cut(s) | 1028, 1349, 1728, 2622, 2961 |
| MslI | CAYNNNNRTG | 2 cut(s) | 305, 2229 |
| MspA1I | CMGCKG | 2 cut(s) | 451, 1112 |
| MspCI | CTTAAG | 1 cut(s) | 323 |
| MspI | CCGG | 4 cut(s) | 87, 177, 483, 665 |
| MunI | CAATTG | 2 cut(s) | 1587, 1968 |
| Mva1269I | GAATGC | 2 cut(s) | 1228, 3000 |
| MvnI | CGCG | 5 cut(s) | 27, 49, 64, 1263, 1701 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 361, 457, 2273, 2375 |
| NarI | GGCGCC | 1 cut(s) | 446 |
| NciI | CCSGG | 4 cut(s) | 178, 483, 484, 666 |
| NdeII | GATC | 7 cut(s) | 54, 141, 1369, 1594, 1629, 2167, 2197 |
| NlaIII | CATG | 7 cut(s) | 170, 1526, 1967, 2869, 3021, 3032, 3084 |
| NlaIV | GGNNCC | 4 cut(s) | 447, 669, 1755, 3011 |
| NmeAIII | GCCGAG | 2 cut(s) | 101, 385 |
| NmuCI | GTSAC | 4 cut(s) | 1545, 1617, 1983, 2590 |
| NsiI | ATGCAT | 1 cut(s) | 688 |
| NspI | RCATGY | 2 cut(s) | 3021, 3032 |
| OliI | CACNNNNGTG | 1 cut(s) | 2229 |
| PaeI | GCATGC | 1 cut(s) | 3032 |
| PaeR7I | CTCGAG | 1 cut(s) | 1836 |
| PaqCI | CACCTGC | 1 cut(s) | 2782 |
| PasI | CCCWGGG | 1 cut(s) | 1890 |
| PciI | ACATGT | 1 cut(s) | 3017 |
| PciSI | GCTCTTC | 2 cut(s) | 1218, 3141 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 372 |
| PctI | GAATGC | 2 cut(s) | 1228, 3000 |
| PdmI | GAANNNNTTC | 5 cut(s) | 1028, 1349, 1728, 2622, 2961 |
| PfeI | GAWTC | 8 cut(s) | 83, 106, 371, 803, 962, 1391, 2324, 3112 |
| PflFI | GACNNNGTC | 1 cut(s) | 787 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 611 |
| PfoI | TCCNGGA | 1 cut(s) | 2293 |
| PluTI | GGCGCC | 1 cut(s) | 449 |
| PmaCI | CACGTG | 1 cut(s) | 2924 |
| PmlI | CACGTG | 1 cut(s) | 2924 |
| Ppu21I | YACGTR | 1 cut(s) | 2924 |
| PscI | ACATGT | 1 cut(s) | 3017 |
| Psp124BI | GAGCTC | 1 cut(s) | 366 |
| PspCI | CACGTG | 1 cut(s) | 2924 |
| PspFI | CCCAGC | 2 cut(s) | 275, 2552 |
| PspN4I | GGNNCC | 4 cut(s) | 447, 669, 1755, 3011 |
| PspPI | GGNCC | 4 cut(s) | 66, 668, 2204, 3095 |
| PstI | CTGCAG | 2 cut(s) | 2275, 2793 |
| PstNI | CAGNNNCTG | 4 cut(s) | 532, 1340, 2059, 2171 |
| PsuI | RGATCY | 1 cut(s) | 2167 |
| PsyI | GACNNNGTC | 1 cut(s) | 787 |
| PvuII | CAGCTG | 1 cut(s) | 1112 |
| RsaI | GTAC | 5 cut(s) | 161, 209, 267, 2242, 2359 |
| RsaNI | GTAC | 5 cut(s) | 160, 208, 266, 2241, 2358 |
| RseI | CAYNNNNRTG | 2 cut(s) | 305, 2229 |
| SacI | GAGCTC | 1 cut(s) | 366 |
| SapI | GCTCTTC | 2 cut(s) | 1218, 3141 |
| Sau3AI | GATC | 7 cut(s) | 54, 141, 1369, 1594, 1629, 2167, 2197 |
| Sau96I | GGNCC | 4 cut(s) | 66, 668, 2204, 3095 |
| SduI | GDGCHC | 4 cut(s) | 314, 366, 1609, 2505 |
| SexAI | ACCWGGT | 2 cut(s) | 495, 2446 |
| SfaNI | GCATC | 9 cut(s) | 673, 1276, 1505, 1855, 2120, 2323, 2365, 2941, 3184 |
| SfcI | CTRYAG | 3 cut(s) | 2250, 2271, 2789 |
| SfoI | GGCGCC | 1 cut(s) | 447 |
| Sfr274I | CTCGAG | 1 cut(s) | 1836 |
| SinI | GGWCC | 4 cut(s) | 66, 668, 2204, 3095 |
| SlaI | CTCGAG | 1 cut(s) | 1836 |
| SmaI | CCCGGG | 1 cut(s) | 484 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 305, 2229 |
| SmlI | CTYRAG | 4 cut(s) | 323, 335, 1836, 2933 |
| SmoI | CTYRAG | 4 cut(s) | 323, 335, 1836, 2933 |
| SphI | GCATGC | 1 cut(s) | 3032 |
| SsiI | CCGC | 9 cut(s) | 27, 47, 64, 449, 554, 707, 1261, 3032, 3202 |
| SspDI | GGCGCC | 1 cut(s) | 445 |
| SspI | AATATT | 1 cut(s) | 3246 |
| SspMI | CTAG | 5 cut(s) | 474, 915, 1743, 2049, 2676 |
| SstI | GAGCTC | 1 cut(s) | 366 |
| StyI | CCWWGG | 2 cut(s) | 1647, 3162 |
| TaiI | ACGT | 5 cut(s) | 548, 1562, 2926, 3040, 3211 |
| TatI | WGTACW | 4 cut(s) | 159, 207, 265, 2240 |
| TauI | GCSGC | 2 cut(s) | 49, 451 |
| TfiI | GAWTC | 8 cut(s) | 83, 106, 371, 803, 962, 1391, 2324, 3112 |
| TscAI | CASTG | 4 cut(s) | 604, 996, 1345, 2250 |
| TseFI | GTSAC | 4 cut(s) | 1545, 1617, 1983, 2590 |
| Tsp45I | GTSAC | 4 cut(s) | 1545, 1617, 1983, 2590 |
| TspGWI | ACGGA | 5 cut(s) | 50, 1343, 2230, 2434, 3115 |
| TspMI | CCCGGG | 1 cut(s) | 482 |
| TspRI | CASTG | 4 cut(s) | 604, 996, 1345, 2250 |
| Tth111I | GACNNNGTC | 1 cut(s) | 787 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 611 |
| Vha464I | CTTAAG | 1 cut(s) | 323 |
| VneI | GTGCAC | 1 cut(s) | 1605 |
| VpaK11BI | GGWCC | 4 cut(s) | 66, 668, 2204, 3095 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 588, 2307, 2789 |
| XapI | RAATTY | 9 cut(s) | 1040, 1345, 1738, 1791, 1845, 2009, 2713, 2743, 2893 |
| XceI | RCATGY | 2 cut(s) | 3021, 3032 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 283 |
| XhoI | CTCGAG | 1 cut(s) | 1836 |
| XmaI | CCCGGG | 1 cut(s) | 482 |
| XmiI | GTMKAC | 2 cut(s) | 2494, 2659 |
| XmnI | GAANNNNTTC | 5 cut(s) | 1028, 1349, 1728, 2622, 2961 |
| XspI | CTAG | 5 cut(s) | 474, 915, 1743, 2049, 2676 |
| ZraI | GACGTC | 2 cut(s) | 546, 1560 |
| Zsp2I | ATGCAT | 1 cut(s) | 688 |