Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000141

Physical Location & Seq

Genomic Coordinates Forward (+)

27148196 .. 27149393

Gene Structure 1198 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1245800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGGAGTCCATTAACCAGTACTACTCCAAATGTATCTGTTTGGCTTTGATCTTCATGTTGGGAGCCTTGTCTTCTCAAGCCACTTCTCGCGCTCTCCAAGATGCATCAATGTATGGGAAACACGAGCAATGGATGGCTCGTTATGGACGTGTATATACCGACATTAACGAGAAGGAGAAGCGTTTCAACATATTCAAGGAAAATGTGGCATTTATTGAATCTTCCAATAAGGATGCATACAAACTTTACAAATTGAGCGTCAATCAATTTGCAGACCTTACAAATGAAGAATTCAAAGCCTCAAGAAATGGATTCATGGGGCACGAATGTTCAGCAAAGACGACTTGTTTCAAATATGAAAATGTTACTGCACCACCAACTGTAGATTGGAGAAAGAAAGGAGCCGTTACACCCATCAAGGACCAAGGCCAATGTGGATGTTGTTGGGCTTTTTCAGCAGTGGCAGCAATGGAAGGGATTACAAAGCTCACAACTGGTAAATTGATATCTTTGTCTGAGCAAGAGCTGGTTGATTGTGACACAAGCGGTGTAGACCAAGGTTGTCAGGGTGGTTTGATGGATGATGCATTTCAGTTCATCAATCAAAACCATGGGCTTAGTACCGAGGCTAATTACCCGTACACTGGAGTTGATGGTACTTGCAACACCAAGAAGGAGGCCAATCGTGCAGCCAAGATAACTGGTCATGAAGATGTGCCTGCAAACCGTGAAGATGCCCTAATGAAGGCCGTTGCTAATCAACCAATTTCTGTTGCCATTGACGCTAGCGGTTCCGACTTTCAATTCTATTCAAGTGGTGGAGTTAGTGATGATGGTACCAAATATTGGTTGGTGAAGAATTCATGGGGCAAAGAATGGGGTGAAGAAGGGTACATAAGAATGCAGAGAGGTATTGAGGCAAAGGAAGGTCTTTGTGGCATTGCTATGGAAGCTTCCTACCCCACTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

324

Amino Acids

35.65

Weight (kDa)

5.31

Isoelectric Point (pI)

21.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	41 - 98	1.5e-18	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	125 - 273	1.4e-56	Papain family cysteine protease
Peptidase_C1	PF00112	277 - 322	4.3e-14	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000141)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22160
fragaria_vesca	FvH4_1g23580 FvH4_1g25982 FvH4_2g17070 FvH4_3g40500 FvH4_6g27180 FvH4_6g27320 FvH4_6g33300 FvH4_6g33310 FvH4_7g09480 FvH4_7g13000 FvH4_7g13320 FvH4_7g13600
malus_domestica	MD00G1208000.v1.1 MD09G1238200.v1.1 MD09G1238600.v1.1 MD09G1238800.v1.1 MD09G1239100.v1.1 MD16G1226900.v1.1 MD16G1242700.v1.1 MD16G1244200.v1.1 MD16G1245500.v1.1 MD16G1245800.v1.1 MD17G1231600.v1.1 MD17G1231700.v1.1 MD17G1231800.v1.1 MD17G1232200.v1.1 MD17G1232300.v1.1 MD17G1232400.v1.1
prunus_persica	Prupe.1G072300_v2.0.a1 Prupe.1G072400_v2.0.a1 Prupe.3G106300_v2.0.a1 Prupe.3G106400_v2.0.a1 Prupe.3G119400_v2.0.a1 Prupe.3G120600_v2.0.a1 Prupe.3G121900_v2.0.a1 Prupe.3G122000_v2.0.a1 Prupe.3G122100_v2.0.a1 Prupe.3G122200_v2.0.a1 Prupe.3G122700_v2.0.a1 Prupe.5G055900_v2.0.a1 Prupe.5G056500_v2.0.a1
pyrus_communis	pycom09g15770 pycom09g15780 pycom09g15790 pycom16g20640 pycom17g18390
rosa_chinensis	RchiOBHm_Chr1g0352141 RchiOBHm_Chr1g0352581 RchiOBHm_Chr1g0352601 RchiOBHm_Chr1g0352621 RchiOBHm_Chr1g0352631 RchiOBHm_Chr2g0119131 RchiOBHm_Chr2g0128691 RchiOBHm_Chr2g0128701 RchiOBHm_Chr2g0129151 RchiOBHm_Chr2g0129221 RchiOBHm_Chr2g0129401 RchiOBHm_Chr2g0129421 RchiOBHm_Chr2g0129431 RchiOBHm_Chr2g0129471 RchiOBHm_Chr2g0129481 RchiOBHm_Chr2g0129491 RchiOBHm_Chr2g0143051 RchiOBHm_Chr2g0143121 RchiOBHm_Chr2g0143171 RchiOBHm_Chr3g0457341 RchiOBHm_Chr5g0049011 RchiOBHm_Chr6g0281281 RchiOBHm_Chr6g0281311
rosa_laevigata	RLG00000012975 RLG00000012976 RLG00000012979 RLG00000018447 RLG00000019018 RLG00000019061 RLG00000019066 RLG00000019078 RLG00000019079 RLG00000019080 RLG00000019083 RLG00000019085 RLG00000020009 RLG00000020010 RLG00000025234 RLG00000028326 RLG00000028327 RLG00000028328 RLG00000028329 RLG00000028357 RLG00000031784
rosa_multiflora	Rmu_co8169188.1_g000001 Rmu_co8261007.1_g000001 Rmu_co8314361.1_g000001 Rmu_sc0000109.1_g000014 Rmu_sc0000638.1_g000020 Rmu_sc0000847.1_g000020 Rmu_sc0000847.1_g000028 Rmu_sc0000847.1_g000037 Rmu_sc0000847.1_g000046 Rmu_sc0000911.1_g000034 Rmu_sc0000999.1_g000021 Rmu_sc0001171.1_g000013 Rmu_sc0001171.1_g000018 Rmu_sc0001171.1_g000019 Rmu_sc0001653.1_g000004 Rmu_sc0002277.1_g000005 Rmu_sc0003479.1_g000001 Rmu_sc0005118.1_g000006 Rmu_sc0005118.1_g000008 Rmu_sc0005227.1_g000012 Rmu_sc0005442.1_g000010 Rmu_sc0005514.1_g000005 Rmu_sc0005996.1_g000014 Rmu_sc0008800.1_g000002 Rmu_sc0008800.1_g000010 Rmu_sc0008800.1_g000014
rosa_roxburghii	Rroxscaffold_1G00065560 Rroxscaffold_2G00102080 Rroxscaffold_2G00102090 Rroxscaffold_2G00102110 Rroxscaffold_2G00114560 Rroxscaffold_2G00114570 Rroxscaffold_2G00114610 Rroxscaffold_2G00114620 Rroxscaffold_2G00114640 Rroxscaffold_2G00114740 Rroxscaffold_2G00114790 Rroxscaffold_2G00115860 Rroxscaffold_2G00124490 Rroxscaffold_4G00302260 Rroxscaffold_4G00302530 Rroxscaffold_4G00302550 Rroxscaffold_4G00302560 Rroxscaffold_4G00302620 Rroxscaffold_6G00421980 Rroxscaffold_7G00187140 Rroxscaffold_7G00187170
rosa_rugosa	Rorug01G0225000 Rorug01G0230200 Rorug02G0220500 Rorug02G0280000 Rorug02G0285200 Rorug02G0285400 Rorug02G0286300 Rorug02G0286300 Rorug02G0286400 Rorug02G0286900 Rorug02G0377800 Rorug02G0377900 Rorug02G0378000 Rorug02G0378000 Rorug02G0378800 Rorug03G0019800 Rorug04G0452700 Rorug06G0140300 Rorug06G0140500 Rorug06G0140600
rosa_samantha	Rh1CG223600 Rh1CG227200 Rh1CG227500 Rh1CG227600 Rh2BG341200 Rh2BG345300 Rh2BG440200 Rh2DG284500 Rh2DG357900 Rh2DG362100 Rh2DG362600 Rh2DG364500 Rh2DG364600 Rh2DG364700 Rh2DG364800 Rh2DG364900 Rh2DG365000 Rh2DG365400 Rh2DG450400 Rh3AG080000 Rh3BG081900 Rh5AG322100 Rh5BG079800 Rh6AG252100 Rh6BG255600
rosa_wichuraiana	Rw1G020770 Rw1G021290 Rw2G022120 Rw2G026940 Rw2G027360 Rw2G027370 Rw2G027950 Rw2G027960 Rw2G027970 Rw2G035220 Rw3G006890 Rw6G021900 Rw6G021930 Rw6G021950 Rw6G021970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	836
AccB1I	GGYRCC	1 cut(s)	836
AccB7I	CCANNNNNTGG	1 cut(s)	846
AccI	GTMKAC	1 cut(s)	552
AccII	CGCG	1 cut(s)	90
AciI	CCGC	2 cut(s)	546, 789
AcsI	RAATTY	2 cut(s)	290, 859
AfaI	GTAC	6 cut(s)	20, 622, 641, 658, 838, 893
AfiI	CCNNNNNNNGG	3 cut(s)	644, 745, 846
AflIII	ACRYGT	1 cut(s)	148
AgsI	TTSAA	7 cut(s)	187, 196, 218, 295, 352, 803, 813
AjiI	CACGTC	1 cut(s)	149
AluBI	AGCT	3 cut(s)	487, 526, 953
AluI	AGCT	3 cut(s)	487, 526, 953
AoxI	GGCC	3 cut(s)	427, 678, 747
ApeKI	GCWGC	2 cut(s)	464, 689
ApoI	RAATTY	2 cut(s)	290, 859
ArsI	GACNNNNNNTTYG	2 cut(s)	243, 275
Asp700I	GAANNNNTTC	1 cut(s)	182
Asp718I	GGTACC	1 cut(s)	836
AspLEI	GCGC	1 cut(s)	92
AspS9I	GGNCC	1 cut(s)	421
AsuHPI	GGTGA	2 cut(s)	865, 893
AsuNHI	GCTAGC	1 cut(s)	785
AvaII	GGWCC	1 cut(s)	421
BaeGI	GKGCMC	1 cut(s)	324
BanI	GGYRCC	1 cut(s)	836
BauI	CACGAG	1 cut(s)	122
BbsI	GAAGAC	1 cut(s)	63
BbvI	GCAGC	2 cut(s)	476, 701
BccI	CCATC	5 cut(s)	127, 422, 571, 647, 827
BceAI	ACGGC	2 cut(s)	389, 734
BfaI	CTAG	1 cut(s)	786
BfmI	CTRYAG	1 cut(s)	381
BisI	GCNGC	2 cut(s)	465, 690
BlsI	GCNGC	2 cut(s)	466, 691
BmcAI	AGTACT	1 cut(s)	20
Bme18I	GGWCC	1 cut(s)	421
BmgBI	CACGTC	1 cut(s)	149
BmgT120I	GGNCC	1 cut(s)	421
BmiI	GGNNCC	4 cut(s)	64, 403, 793, 838
BmsI	GCATC	5 cut(s)	91, 113, 223, 574, 724
BmtI	GCTAGC	1 cut(s)	789
BpiI	GAAGAC	1 cut(s)	63
BpmI	CTGGAG	1 cut(s)	666
BpuEI	CTTGAG	2 cut(s)	60, 286
BsaJI	CCNNGG	4 cut(s)	424, 556, 610, 624
Bsc4I	CCNNNNNNNGG	3 cut(s)	644, 745, 846
Bse1I	ACTGG	4 cut(s)	16, 499, 649, 706
Bse3DI	GCAATG	3 cut(s)	134, 474, 939
BseDI	CCNNGG	4 cut(s)	424, 556, 610, 624
BseGI	GGATG	4 cut(s)	138, 238, 443, 586
BseLI	CCNNNNNNNGG	3 cut(s)	644, 745, 846
BseMI	GCAATG	3 cut(s)	134, 474, 939
BseMII	CTCAG	1 cut(s)	507
BseNI	ACTGG	4 cut(s)	16, 499, 649, 706
BseSI	GKGCMC	1 cut(s)	324
BseXI	GCAGC	2 cut(s)	476, 701
BsgI	GTGCAG	2 cut(s)	354, 708
Bsh1236I	CGCG	1 cut(s)	90
BshFI	GGCC	3 cut(s)	429, 680, 749
BshNI	GGYRCC	1 cut(s)	836
BslI	CCNNNNNNNGG	3 cut(s)	644, 745, 846
BsmI	GAATGC	1 cut(s)	906
BsnI	GGCC	3 cut(s)	429, 680, 749
Bsp1286I	GDGCHC	1 cut(s)	324
Bsp143I	GATC	1 cut(s)	48
Bsp19I	CCATGG	1 cut(s)	610
BspACI	CCGC	2 cut(s)	546, 789
BspANI	GGCC	3 cut(s)	429, 680, 749
BspCNI	CTCAG	1 cut(s)	508
BspFNI	CGCG	1 cut(s)	90
BspHI	TCATGA	1 cut(s)	706
BspLI	GGNNCC	4 cut(s)	64, 403, 793, 838
BspOI	GCTAGC	1 cut(s)	789
BspT107I	GGYRCC	1 cut(s)	836
BsrDI	GCAATG	3 cut(s)	134, 474, 939
BsrI	ACTGG	4 cut(s)	16, 499, 649, 706
BssECI	CCNNGG	4 cut(s)	424, 556, 610, 624
BssMI	GATC	1 cut(s)	48
BssSI	CACGAG	1 cut(s)	122
BssT1I	CCWWGG	3 cut(s)	424, 556, 610
Bst2BI	CACGAG	1 cut(s)	122
Bst4CI	ACNGT	2 cut(s)	382, 728
BstC8I	GCNNGC	2 cut(s)	720, 787
BstDEI	CTNAG	2 cut(s)	516, 617
BstDSI	CCRYGG	1 cut(s)	610
BstENI	CCTNNNNNAGG	1 cut(s)	743
BstF5I	GGATG	4 cut(s)	138, 238, 443, 586
BstFNI	CGCG	1 cut(s)	90
BstHHI	GCGC	1 cut(s)	92
BstKTI	GATC	1 cut(s)	51
BstMBI	GATC	1 cut(s)	48
BstMWI	GCNNNNNNNGC	5 cut(s)	455, 464, 686, 782, 950
BstSFI	CTRYAG	1 cut(s)	381
BstSLI	GKGCMC	1 cut(s)	324
BstUI	CGCG	1 cut(s)	90
BstV1I	GCAGC	2 cut(s)	476, 701
BstV2I	GAAGAC	1 cut(s)	63
BsuRI	GGCC	3 cut(s)	429, 680, 749
BtgI	CCRYGG	1 cut(s)	610
BtrI	CACGTC	1 cut(s)	149
BtsCI	GGATG	4 cut(s)	138, 238, 443, 586
BtsI	GCAGTG	2 cut(s)	465, 963
BtsIMutI	CAGTG	3 cut(s)	465, 642, 963
Cac8I	GCNNGC	2 cut(s)	720, 787
CciI	TCATGA	1 cut(s)	706
CfoI	GCGC	1 cut(s)	92
Cfr13I	GGNCC	1 cut(s)	421
CseI	GACGC	2 cut(s)	247, 791
Csp6I	GTAC	6 cut(s)	19, 621, 640, 657, 837, 892
CviAII	CATG	5 cut(s)	55, 316, 611, 707, 864
CviQI	GTAC	6 cut(s)	19, 621, 640, 657, 837, 892
DdeI	CTNAG	2 cut(s)	516, 617
DpnI	GATC	1 cut(s)	50
DpnII	GATC	1 cut(s)	48
Eco130I	CCWWGG	3 cut(s)	424, 556, 610
Eco32I	GATATC	1 cut(s)	507
Eco47I	GGWCC	1 cut(s)	421
EcoNI	CCTNNNNNAGG	1 cut(s)	743
EcoRI	GAATTC	2 cut(s)	290, 859
EcoRV	GATATC	1 cut(s)	507
EcoT14I	CCWWGG	3 cut(s)	424, 556, 610
EcoT22I	ATGCAT	3 cut(s)	106, 238, 589
ErhI	CCWWGG	3 cut(s)	424, 556, 610
FaeI	CATG	5 cut(s)	58, 319, 614, 710, 867
FatI	CATG	5 cut(s)	54, 315, 610, 706, 863
FblI	GTMKAC	1 cut(s)	552
Fnu4HI	GCNGC	2 cut(s)	465, 690
FokI	GGATG	4 cut(s)	145, 245, 450, 593
Fsp4HI	GCNGC	2 cut(s)	465, 690
FspBI	CTAG	1 cut(s)	786
GlaI	GCGC	1 cut(s)	91
GluI	GCNGC	2 cut(s)	465, 690
GsuI	CTGGAG	1 cut(s)	666
HaeIII	GGCC	3 cut(s)	429, 680, 749
HgaI	GACGC	2 cut(s)	247, 791
HhaI	GCGC	1 cut(s)	92
Hin1II	CATG	5 cut(s)	58, 319, 614, 710, 867
Hin6I	GCGC	1 cut(s)	90
HinP1I	GCGC	1 cut(s)	90
HindIII	AAGCTT	1 cut(s)	951
HinfI	GANTC	3 cut(s)	5, 218, 312
HphI	GGTGA	2 cut(s)	865, 893
Hpy166II	GTNNAC	2 cut(s)	553, 642
Hpy188I	TCNGA	2 cut(s)	517, 796
Hpy188III	TCNNGA	2 cut(s)	303, 707
Hpy8I	GTNNAC	2 cut(s)	553, 642
HpyAV	CCTTC	6 cut(s)	166, 467, 667, 739, 881, 920
HpyCH4III	ACNGT	2 cut(s)	382, 728
HpyCH4IV	ACGT	1 cut(s)	148
HpyF10VI	GCNNNNNNNGC	5 cut(s)	455, 464, 686, 782, 950
HpyF3I	CTNAG	2 cut(s)	516, 617
HpySE526I	ACGT	1 cut(s)	148
Hsp92II	CATG	5 cut(s)	58, 319, 614, 710, 867
HspAI	GCGC	1 cut(s)	90
KpnI	GGTACC	1 cut(s)	840
Kzo9I	GATC	1 cut(s)	48
LmnI	GCTCC	2 cut(s)	62, 401
LpnPI	CCDG	7 cut(s)	29, 480, 512, 551, 630, 687, 732
Lsp1109I	GCAGC	2 cut(s)	476, 701
LweI	GCATC	5 cut(s)	91, 113, 223, 574, 724
MaeI	CTAG	1 cut(s)	786
MaeII	ACGT	1 cut(s)	148
MaeIII	GTNAC	3 cut(s)	364, 406, 536
MalI	GATC	1 cut(s)	50
MboI	GATC	1 cut(s)	48
MboII	GAAGA	8 cut(s)	43, 63, 213, 299, 722, 743, 868, 896
MhlI	GDGCHC	1 cut(s)	324
MluCI	AATT	8 cut(s)	251, 266, 290, 500, 631, 765, 803, 859
MlyI	GAGTC	1 cut(s)	14
MmeI	TCCRAC	1 cut(s)	819
MnlI	CCTC	5 cut(s)	310, 619, 670, 902, 910
Mph1103I	ATGCAT	3 cut(s)	106, 238, 589
MroXI	GAANNNNTTC	1 cut(s)	182
MseI	TTAA	2 cut(s)	12, 165
MslI	CAYNNNNRTG	3 cut(s)	711, 899, 944
Mva1269I	GAATGC	1 cut(s)	906
MvnI	CGCG	1 cut(s)	90
MwoI	GCNNNNNNNGC	5 cut(s)	455, 464, 686, 782, 950
NcoI	CCATGG	1 cut(s)	610
NdeII	GATC	1 cut(s)	48
NheI	GCTAGC	1 cut(s)	785
NlaIII	CATG	5 cut(s)	58, 319, 614, 710, 867
NlaIV	GGNNCC	4 cut(s)	64, 403, 793, 838
NmuCI	GTSAC	1 cut(s)	536
NsiI	ATGCAT	3 cut(s)	106, 238, 589
PagI	TCATGA	1 cut(s)	706
PcsI	WCGNNNNNNNCGW	1 cut(s)	145
PctI	GAATGC	1 cut(s)	906
PdmI	GAANNNNTTC	1 cut(s)	182
PfeI	GAWTC	2 cut(s)	218, 312
PflMI	CCANNNNNTGG	1 cut(s)	846
PkrI	GCNGC	2 cut(s)	466, 691
PleI	GAGTC	1 cut(s)	13
PpsI	GAGTC	1 cut(s)	13
PspN4I	GGNNCC	4 cut(s)	64, 403, 793, 838
PspPI	GGNCC	1 cut(s)	421
RsaI	GTAC	6 cut(s)	20, 622, 641, 658, 838, 893
RsaNI	GTAC	6 cut(s)	19, 621, 640, 657, 837, 892
RseI	CAYNNNNRTG	3 cut(s)	711, 899, 944
SaqAI	TTAA	2 cut(s)	12, 165
SatI	GCNGC	2 cut(s)	465, 690
Sau3AI	GATC	1 cut(s)	48
Sau96I	GGNCC	1 cut(s)	421
ScaI	AGTACT	1 cut(s)	20
SchI	GAGTC	1 cut(s)	14
SduI	GDGCHC	1 cut(s)	324
SetI	ASST	8 cut(s)	151, 279, 489, 528, 562, 913, 931, 955
SfaNI	GCATC	5 cut(s)	91, 113, 223, 574, 724
SfcI	CTRYAG	1 cut(s)	381
SinI	GGWCC	1 cut(s)	421
SmiMI	CAYNNNNRTG	3 cut(s)	711, 899, 944
SmlI	CTYRAG	2 cut(s)	75, 301
SmoI	CTYRAG	2 cut(s)	75, 301
Sse9I	AATT	8 cut(s)	251, 266, 290, 500, 631, 765, 803, 859
SsiI	CCGC	2 cut(s)	546, 789
SspI	AATATT	1 cut(s)	845
SspMI	CTAG	1 cut(s)	786
StyI	CCWWGG	3 cut(s)	424, 556, 610
TaaI	ACNGT	2 cut(s)	382, 728
TaiI	ACGT	1 cut(s)	151
TasI	AATT	8 cut(s)	251, 266, 290, 500, 631, 765, 803, 859
TatI	WGTACW	1 cut(s)	18
TfiI	GAWTC	2 cut(s)	218, 312
Tru1I	TTAA	2 cut(s)	12, 165
Tru9I	TTAA	2 cut(s)	12, 165
TscAI	CASTG	3 cut(s)	465, 649, 970
TseFI	GTSAC	1 cut(s)	536
TseI	GCWGC	2 cut(s)	464, 689
Tsp45I	GTSAC	1 cut(s)	536
TspDTI	ATGAA	8 cut(s)	43, 300, 304, 372, 586, 723, 758, 852
TspRI	CASTG	3 cut(s)	465, 649, 970
Van91I	CCANNNNNTGG	1 cut(s)	846
VpaK11BI	GGWCC	1 cut(s)	421
XagI	CCTNNNNNAGG	1 cut(s)	743
XapI	RAATTY	2 cut(s)	290, 859
XcmI	CCANNNNNNNNNTGG	3 cut(s)	384, 431, 847
XmiI	GTMKAC	1 cut(s)	552
XmnI	GAANNNNTTC	1 cut(s)	182
XspI	CTAG	1 cut(s)	786
ZrmI	AGTACT	1 cut(s)	20
Zsp2I	ATGCAT	3 cut(s)	106, 238, 589

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.