Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000141

Physical Location & Seq

Genomic Coordinates Forward (+)

30377485 .. 30378636

Gene Structure 1152 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1238800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGGGGTTCATTAACCTGCAGCCGTGGAAATATATGTGCTTGAGCTTGATCCTCATGCTGGGGGCCTGGTCATCCGGAGCCACTTCTCGCACTCTACAAGATGCATCGATGTATGGGAGATACGAGCAATGGTTGACTCGTTATGGTCGCGTATATAACGACGTTAATGAGAAGGAGACTCGTTTCAAGATATTTAAGGAAAATGTGGCGTTTATAGAATCTTCTAATAAGGATGCCAACAAACCTTACAGATTAAGTGTCAATCAATTTGCAGACCTTACAAATGAAGAGTTCAAAGCCTCAAGAAATGGATTCAAGGGACACGAGTGCTCCACGAAGACTTCTTTCAAATATGAAAATTTGACTGCATCATTACCAGCAACAATGGATTGGAGAAAGAAAGGAGCTGTAACCCCCATCAAGGACCAAGGCCAATGCGGTAGATGCTGTTGGGCTTTTTCAGCAGTGGCCGCCACGGAAGGAATTACACAGCTTACAACTGGGAAATTGATCTCTTTGTCTGAGCAAGAGCTCGTTGATTGTGACACCGCTGGTGAAGACCAAGGATGTGAGGGCGGCTTGATGGATGATGCCTTCCAGTTCATCCAACAAAATCGCGGGATTAGCACAGAAGCTAATTACCCTTACGACGGCGTTGATGGTACATGTAACGCCAAGAAGGAAGCCAGCCGTGCAGCCAAGATAACTGGCTTTGAAGATGTCCCTGCAAACAGTGAAAAGGCCCTTCTTACCGCAGTAGCTCACCAACCTGTTTCTGTTGCCATTGATGCTAGTGGTTCTGACTTCCAGTTCTATTCTAGTGGTGTCTTTACCGGGACCTGTGGAACGAGCCTTGACCATGGTGTTACCGCTGTTGGTTATGGCGTGAGCGAGGATGGGACTAAGTATTGGCTAGTGAAGAACTCATGGGGTACGGAATGGGGTGAAGCTGGATACATAAGAATGCAGAGAGATGTTGCTGCGGGAGAAGGACTTTGTGGCATTGCTATGGCTGCCTCTTACCCCACAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

345

Amino Acids

37.56

Weight (kDa)

5.12

Isoelectric Point (pI)

22.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	41 - 98	1.5e-18	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	125 - 343	2.8e-83	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000141)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22160
fragaria_vesca	FvH4_1g23580 FvH4_1g25982 FvH4_2g17070 FvH4_3g40500 FvH4_6g27180 FvH4_6g27320 FvH4_6g33300 FvH4_6g33310 FvH4_7g09480 FvH4_7g13000 FvH4_7g13320 FvH4_7g13600
malus_domestica	MD00G1208000.v1.1 MD09G1238200.v1.1 MD09G1238600.v1.1 MD09G1238800.v1.1 MD09G1239100.v1.1 MD16G1226900.v1.1 MD16G1242700.v1.1 MD16G1244200.v1.1 MD16G1245500.v1.1 MD16G1245800.v1.1 MD17G1231600.v1.1 MD17G1231700.v1.1 MD17G1231800.v1.1 MD17G1232200.v1.1 MD17G1232300.v1.1 MD17G1232400.v1.1
prunus_persica	Prupe.1G072300_v2.0.a1 Prupe.1G072400_v2.0.a1 Prupe.3G106300_v2.0.a1 Prupe.3G106400_v2.0.a1 Prupe.3G119400_v2.0.a1 Prupe.3G120600_v2.0.a1 Prupe.3G121900_v2.0.a1 Prupe.3G122000_v2.0.a1 Prupe.3G122100_v2.0.a1 Prupe.3G122200_v2.0.a1 Prupe.3G122700_v2.0.a1 Prupe.5G055900_v2.0.a1 Prupe.5G056500_v2.0.a1
pyrus_communis	pycom09g15770 pycom09g15780 pycom09g15790 pycom16g20640 pycom17g18390
rosa_chinensis	RchiOBHm_Chr1g0352141 RchiOBHm_Chr1g0352581 RchiOBHm_Chr1g0352601 RchiOBHm_Chr1g0352621 RchiOBHm_Chr1g0352631 RchiOBHm_Chr2g0119131 RchiOBHm_Chr2g0128691 RchiOBHm_Chr2g0128701 RchiOBHm_Chr2g0129151 RchiOBHm_Chr2g0129221 RchiOBHm_Chr2g0129401 RchiOBHm_Chr2g0129421 RchiOBHm_Chr2g0129431 RchiOBHm_Chr2g0129471 RchiOBHm_Chr2g0129481 RchiOBHm_Chr2g0129491 RchiOBHm_Chr2g0143051 RchiOBHm_Chr2g0143121 RchiOBHm_Chr2g0143171 RchiOBHm_Chr3g0457341 RchiOBHm_Chr5g0049011 RchiOBHm_Chr6g0281281 RchiOBHm_Chr6g0281311
rosa_laevigata	RLG00000012975 RLG00000012976 RLG00000012979 RLG00000018447 RLG00000019018 RLG00000019061 RLG00000019066 RLG00000019078 RLG00000019079 RLG00000019080 RLG00000019083 RLG00000019085 RLG00000020009 RLG00000020010 RLG00000025234 RLG00000028326 RLG00000028327 RLG00000028328 RLG00000028329 RLG00000028357 RLG00000031784
rosa_multiflora	Rmu_co8169188.1_g000001 Rmu_co8261007.1_g000001 Rmu_co8314361.1_g000001 Rmu_sc0000109.1_g000014 Rmu_sc0000638.1_g000020 Rmu_sc0000847.1_g000020 Rmu_sc0000847.1_g000028 Rmu_sc0000847.1_g000037 Rmu_sc0000847.1_g000046 Rmu_sc0000911.1_g000034 Rmu_sc0000999.1_g000021 Rmu_sc0001171.1_g000013 Rmu_sc0001171.1_g000018 Rmu_sc0001171.1_g000019 Rmu_sc0001653.1_g000004 Rmu_sc0002277.1_g000005 Rmu_sc0003479.1_g000001 Rmu_sc0005118.1_g000006 Rmu_sc0005118.1_g000008 Rmu_sc0005227.1_g000012 Rmu_sc0005442.1_g000010 Rmu_sc0005514.1_g000005 Rmu_sc0005996.1_g000014 Rmu_sc0008800.1_g000002 Rmu_sc0008800.1_g000010 Rmu_sc0008800.1_g000014
rosa_roxburghii	Rroxscaffold_1G00065560 Rroxscaffold_2G00102080 Rroxscaffold_2G00102090 Rroxscaffold_2G00102110 Rroxscaffold_2G00114560 Rroxscaffold_2G00114570 Rroxscaffold_2G00114610 Rroxscaffold_2G00114620 Rroxscaffold_2G00114640 Rroxscaffold_2G00114740 Rroxscaffold_2G00114790 Rroxscaffold_2G00115860 Rroxscaffold_2G00124490 Rroxscaffold_4G00302260 Rroxscaffold_4G00302530 Rroxscaffold_4G00302550 Rroxscaffold_4G00302560 Rroxscaffold_4G00302620 Rroxscaffold_6G00421980 Rroxscaffold_7G00187140 Rroxscaffold_7G00187170
rosa_rugosa	Rorug01G0225000 Rorug01G0230200 Rorug02G0220500 Rorug02G0280000 Rorug02G0285200 Rorug02G0285400 Rorug02G0286300 Rorug02G0286300 Rorug02G0286400 Rorug02G0286900 Rorug02G0377800 Rorug02G0377900 Rorug02G0378000 Rorug02G0378000 Rorug02G0378800 Rorug03G0019800 Rorug04G0452700 Rorug06G0140300 Rorug06G0140500 Rorug06G0140600
rosa_samantha	Rh1CG223600 Rh1CG227200 Rh1CG227500 Rh1CG227600 Rh2BG341200 Rh2BG345300 Rh2BG440200 Rh2DG284500 Rh2DG357900 Rh2DG362100 Rh2DG362600 Rh2DG364500 Rh2DG364600 Rh2DG364700 Rh2DG364800 Rh2DG364900 Rh2DG365000 Rh2DG365400 Rh2DG450400 Rh3AG080000 Rh3BG081900 Rh5AG322100 Rh5BG079800 Rh6AG252100 Rh6BG255600
rosa_wichuraiana	Rw1G020770 Rw1G021290 Rw2G022120 Rw2G026940 Rw2G027360 Rw2G027370 Rw2G027950 Rw2G027960 Rw2G027970 Rw2G035220 Rw3G006890 Rw6G021900 Rw6G021930 Rw6G021950 Rw6G021970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	24
AccII	CGCG	2 cut(s)	150, 618
AccIII	TCCGGA	1 cut(s)	74
AciI	CCGC	8 cut(s)	438, 471, 549, 576, 618, 753, 870, 983
AclWI	GGATC	1 cut(s)	43
AcoI	YGGCCR	1 cut(s)	468
AcsI	RAATTY	1 cut(s)	358
AfaI	GTAC	2 cut(s)	664, 934
AfiI	CCNNNNNNNGG	3 cut(s)	58, 421, 650
AflIII	ACRYGT	1 cut(s)	665
AgsI	TTSAA	5 cut(s)	187, 295, 316, 349, 716
AjnI	CCWGG	1 cut(s)	65
AluBI	AGCT	8 cut(s)	45, 407, 493, 532, 635, 761, 950, 1031
AluI	AGCT	8 cut(s)	45, 407, 493, 532, 635, 761, 950, 1031
Alw21I	GWGCWC	2 cut(s)	332, 534
Alw26I	GTCTC	1 cut(s)	170
AlwI	GGATC	1 cut(s)	43
Aor13HI	TCCGGA	1 cut(s)	74
AoxI	GGCC	4 cut(s)	63, 430, 468, 741
ApeKI	GCWGC	4 cut(s)	19, 695, 980, 1013
ApoI	RAATTY	1 cut(s)	358
AspS9I	GGNCC	4 cut(s)	63, 424, 742, 837
AsuC2I	CCSGG	1 cut(s)	835
AsuHPI	GGTGA	3 cut(s)	566, 755, 956
AvaII	GGWCC	2 cut(s)	424, 837
BanII	GRGCYC	1 cut(s)	534
BauI	CACGAG	1 cut(s)	323
BbsI	GAAGAC	2 cut(s)	344, 564
Bbv12I	GWGCWC	2 cut(s)	332, 534
BbvI	GCAGC	4 cut(s)	31, 707, 967, 1000
BccI	CCATC	4 cut(s)	425, 577, 653, 890
BceAI	ACGGC	3 cut(s)	7, 667, 675
BciT130I	CCWGG	1 cut(s)	67
BciVI	GTATCC	1 cut(s)	947
BcnI	CCSGG	1 cut(s)	835
BcoDI	GTCTC	1 cut(s)	170
BfaI	CTAG	3 cut(s)	792, 819, 914
BfmI	CTRYAG	1 cut(s)	17
BfuAI	ACCTGC	1 cut(s)	24
BfuI	GTATCC	1 cut(s)	947
BisI	GCNGC	6 cut(s)	20, 471, 577, 696, 981, 1014
BlsI	GCNGC	6 cut(s)	21, 472, 578, 697, 982, 1015
Bme1390I	CCNGG	2 cut(s)	67, 835
Bme18I	GGWCC	2 cut(s)	424, 837
BmgT120I	GGNCC	4 cut(s)	63, 424, 742, 837
BmiI	GGNNCC	3 cut(s)	64, 79, 838
BmrFI	CCNGG	2 cut(s)	67, 835
BmrI	ACTGGG	1 cut(s)	510
BmsI	GCATC	7 cut(s)	91, 113, 223, 377, 434, 580, 778
BmuI	ACTGGG	1 cut(s)	510
BpiI	GAAGAC	2 cut(s)	344, 564
BpuEI	CTTGAG	2 cut(s)	61, 286
BpuMI	CCSGG	1 cut(s)	835
Bsa29I	ATCGAT	1 cut(s)	107
BsaJI	CCNNGG	5 cut(s)	23, 427, 474, 562, 859
BsaWI	WCCGGW	1 cut(s)	74
BsaXI	ACNNNNNCTCC	2 cut(s)	314, 344
Bsc4I	CCNNNNNNNGG	3 cut(s)	58, 421, 650
Bse1I	ACTGG	4 cut(s)	505, 598, 712, 808
Bse3DI	GCAATG	2 cut(s)	134, 1002
BseAI	TCCGGA	1 cut(s)	74
BseBI	CCWGG	1 cut(s)	67
BseCI	ATCGAT	1 cut(s)	107
BseDI	CCNNGG	5 cut(s)	23, 427, 474, 562, 859
BseGI	GGATG	6 cut(s)	71, 238, 572, 592, 603, 901
BseLI	CCNNNNNNNGG	3 cut(s)	58, 421, 650
BseMI	GCAATG	2 cut(s)	134, 1002
BseMII	CTCAG	1 cut(s)	513
BseNI	ACTGG	4 cut(s)	505, 598, 712, 808
BseXI	GCAGC	4 cut(s)	31, 707, 967, 1000
BseYI	CCCAGC	1 cut(s)	58
BsgI	GTGCAG	1 cut(s)	714
Bsh1236I	CGCG	2 cut(s)	150, 618
BshFI	GGCC	4 cut(s)	65, 432, 470, 743
BshVI	ATCGAT	1 cut(s)	107
BsiHKAI	GWGCWC	2 cut(s)	332, 534
BsiSI	CCGG	2 cut(s)	75, 834
BslFI	GGGAC	4 cut(s)	333, 707, 850, 913
BslI	CCNNNNNNNGG	3 cut(s)	58, 421, 650
BsmAI	GTCTC	1 cut(s)	170
BsmFI	GGGAC	4 cut(s)	333, 707, 850, 913
BsmI	GAATGC	1 cut(s)	969
BsnI	GGCC	4 cut(s)	65, 432, 470, 743
Bsp1286I	GDGCHC	2 cut(s)	332, 534
Bsp13I	TCCGGA	1 cut(s)	74
Bsp143I	GATC	2 cut(s)	48, 510
Bsp19I	CCATGG	1 cut(s)	859
BspACI	CCGC	8 cut(s)	438, 471, 549, 576, 618, 753, 870, 983
BspANI	GGCC	4 cut(s)	65, 432, 470, 743
BspCNI	CTCAG	1 cut(s)	514
BspDI	ATCGAT	1 cut(s)	107
BspEI	TCCGGA	1 cut(s)	74
BspFNI	CGCG	2 cut(s)	150, 618
BspLI	GGNNCC	3 cut(s)	64, 79, 838
BspMAI	CTGCAG	1 cut(s)	21
BspMI	ACCTGC	1 cut(s)	24
BspPI	GGATC	1 cut(s)	43
BsrDI	GCAATG	2 cut(s)	134, 1002
BsrI	ACTGG	4 cut(s)	505, 598, 712, 808
BssECI	CCNNGG	5 cut(s)	23, 427, 474, 562, 859
BssMI	GATC	2 cut(s)	48, 510
BssSI	CACGAG	1 cut(s)	323
BssT1I	CCWWGG	3 cut(s)	427, 562, 859
Bst2BI	CACGAG	1 cut(s)	323
Bst2UI	CCWGG	1 cut(s)	67
Bst4CI	ACNGT	1 cut(s)	734
Bst6I	CTCTTC	1 cut(s)	282
BstC8I	GCNNGC	1 cut(s)	688
BstDEI	CTNAG	3 cut(s)	522, 903, 1032
BstDSI	CCRYGG	3 cut(s)	23, 474, 859
BstF5I	GGATG	6 cut(s)	71, 238, 572, 592, 603, 901
BstFNI	CGCG	2 cut(s)	150, 618
BstKTI	GATC	2 cut(s)	51, 513
BstMAI	GTCTC	1 cut(s)	170
BstMBI	GATC	2 cut(s)	48, 510
BstMWI	GCNNNNNNNGC	7 cut(s)	444, 461, 470, 624, 692, 788, 1013
BstNI	CCWGG	1 cut(s)	67
BstNSI	RCATGY	1 cut(s)	669
BstSCI	CCNGG	2 cut(s)	65, 833
BstSFI	CTRYAG	1 cut(s)	17
BstUI	CGCG	2 cut(s)	150, 618
BstV1I	GCAGC	4 cut(s)	31, 707, 967, 1000
BstV2I	GAAGAC	2 cut(s)	344, 564
Bsu15I	ATCGAT	1 cut(s)	107
BsuI	GTATCC	1 cut(s)	947
BsuRI	GGCC	4 cut(s)	65, 432, 470, 743
BsuTUI	ATCGAT	1 cut(s)	107
BtgI	CCRYGG	3 cut(s)	23, 474, 859
BtsCI	GGATG	6 cut(s)	71, 238, 572, 592, 603, 901
BtsI	GCAGTG	1 cut(s)	471
BtsIMutI	CAGTG	2 cut(s)	471, 739
BveI	ACCTGC	1 cut(s)	24
Cac8I	GCNNGC	1 cut(s)	688
Cfr13I	GGNCC	4 cut(s)	63, 424, 742, 837
ClaI	ATCGAT	1 cut(s)	107
Csp6I	GTAC	2 cut(s)	663, 933
CviAII	CATG	4 cut(s)	55, 666, 860, 927
CviQI	GTAC	2 cut(s)	663, 933
DdeI	CTNAG	3 cut(s)	522, 903, 1032
DpnI	GATC	2 cut(s)	50, 512
DpnII	GATC	2 cut(s)	48, 510
EaeI	YGGCCR	1 cut(s)	468
Eam1104I	CTCTTC	1 cut(s)	282
EarI	CTCTTC	1 cut(s)	282
Ecl136II	GAGCTC	1 cut(s)	532
Eco130I	CCWWGG	3 cut(s)	427, 562, 859
Eco24I	GRGCYC	1 cut(s)	534
Eco47I	GGWCC	2 cut(s)	424, 837
Eco53kI	GAGCTC	1 cut(s)	532
EcoICRI	GAGCTC	1 cut(s)	532
EcoO109I	RGGNCCY	3 cut(s)	63, 742, 837
EcoRII	CCWGG	1 cut(s)	65
EcoT14I	CCWWGG	3 cut(s)	427, 562, 859
EcoT22I	ATGCAT	1 cut(s)	106
EcoT38I	GRGCYC	1 cut(s)	534
ErhI	CCWWGG	3 cut(s)	427, 562, 859
FaeI	CATG	4 cut(s)	58, 669, 863, 930
FaqI	GGGAC	4 cut(s)	333, 707, 850, 913
FatI	CATG	4 cut(s)	54, 665, 859, 926
FauI	CCCGC	2 cut(s)	611, 976
Fnu4HI	GCNGC	6 cut(s)	20, 471, 577, 696, 981, 1014
FokI	GGATG	6 cut(s)	58, 245, 579, 590, 599, 908
FriOI	GRGCYC	1 cut(s)	534
Fsp4HI	GCNGC	6 cut(s)	20, 471, 577, 696, 981, 1014
FspBI	CTAG	3 cut(s)	792, 819, 914
GluI	GCNGC	6 cut(s)	20, 471, 577, 696, 981, 1014
GsaI	CCCAGC	1 cut(s)	62
HaeIII	GGCC	4 cut(s)	65, 432, 470, 743
HapII	CCGG	2 cut(s)	75, 834
Hin1II	CATG	4 cut(s)	58, 669, 863, 930
HincII	GTYRAC	1 cut(s)	135
HindII	GTYRAC	1 cut(s)	135
HinfI	GANTC	4 cut(s)	136, 178, 218, 312
HpaII	CCGG	2 cut(s)	75, 834
HphI	GGTGA	3 cut(s)	566, 755, 956
Hpy166II	GTNNAC	1 cut(s)	135
Hpy188I	TCNGA	2 cut(s)	523, 802
Hpy188III	TCNNGA	3 cut(s)	75, 187, 303
Hpy8I	GTNNAC	1 cut(s)	135
Hpy99I	CGWCG	2 cut(s)	164, 653
HpyAV	CCTTC	6 cut(s)	166, 473, 604, 673, 755, 983
HpyCH4III	ACNGT	1 cut(s)	734
HpyCH4IV	ACGT	1 cut(s)	162
HpyCH4V	TGCA	7 cut(s)	19, 104, 272, 368, 695, 728, 967
HpyF10VI	GCNNNNNNNGC	7 cut(s)	444, 461, 470, 624, 692, 788, 1013
HpyF3I	CTNAG	3 cut(s)	522, 903, 1032
HpySE526I	ACGT	1 cut(s)	162
Hsp92II	CATG	4 cut(s)	58, 669, 863, 930
Kpn2I	TCCGGA	1 cut(s)	74
Kzo9I	GATC	2 cut(s)	48, 510
LmnI	GCTCC	3 cut(s)	77, 335, 404
Lsp1109I	GCAGC	4 cut(s)	31, 707, 967, 1000
LweI	GCATC	7 cut(s)	91, 113, 223, 377, 434, 580, 778
MaeI	CTAG	3 cut(s)	792, 819, 914
MaeII	ACGT	1 cut(s)	162
MaeIII	GTNAC	4 cut(s)	409, 542, 668, 865
MalI	GATC	2 cut(s)	50, 512
MboI	GATC	2 cut(s)	48, 510
MboII	GAAGA	6 cut(s)	213, 299, 349, 569, 728, 931
MhlI	GDGCHC	2 cut(s)	332, 534
MluCI	AATT	5 cut(s)	266, 358, 483, 506, 637
MlyI	GAGTC	2 cut(s)	130, 172
MmeI	TCCRAC	1 cut(s)	631
MnlI	CCTC	5 cut(s)	62, 310, 565, 886, 1027
Mph1103I	ATGCAT	1 cut(s)	106
MroI	TCCGGA	1 cut(s)	74
MseI	TTAA	4 cut(s)	12, 165, 195, 254
MslI	CAYNNNNRTG	2 cut(s)	962, 1007
MspA1I	CMGCKG	2 cut(s)	551, 872
MspI	CCGG	2 cut(s)	75, 834
MspR9I	CCNGG	2 cut(s)	67, 835
Mva1269I	GAATGC	1 cut(s)	969
MvaI	CCWGG	1 cut(s)	67
MvnI	CGCG	2 cut(s)	150, 618
MwoI	GCNNNNNNNGC	7 cut(s)	444, 461, 470, 624, 692, 788, 1013
NciI	CCSGG	1 cut(s)	835
NcoI	CCATGG	1 cut(s)	859
NdeII	GATC	2 cut(s)	48, 510
NlaIII	CATG	4 cut(s)	58, 669, 863, 930
NlaIV	GGNNCC	3 cut(s)	64, 79, 838
NmuCI	GTSAC	1 cut(s)	542
NsiI	ATGCAT	1 cut(s)	106
NspI	RCATGY	1 cut(s)	669
PciI	ACATGT	1 cut(s)	665
PctI	GAATGC	1 cut(s)	969
PfeI	GAWTC	2 cut(s)	218, 312
PkrI	GCNGC	6 cut(s)	21, 472, 578, 697, 982, 1015
PleI	GAGTC	2 cut(s)	130, 172
PpsI	GAGTC	2 cut(s)	130, 172
PpuMI	RGGWCCY	1 cut(s)	837
PscI	ACATGT	1 cut(s)	665
Psp124BI	GAGCTC	1 cut(s)	534
Psp5II	RGGWCCY	1 cut(s)	837
Psp6I	CCWGG	1 cut(s)	65
PspFI	CCCAGC	1 cut(s)	58
PspGI	CCWGG	1 cut(s)	65
PspN4I	GGNNCC	3 cut(s)	64, 79, 838
PspPI	GGNCC	4 cut(s)	63, 424, 742, 837
PspPPI	RGGWCCY	1 cut(s)	837
PstI	CTGCAG	1 cut(s)	21
RsaI	GTAC	2 cut(s)	664, 934
RsaNI	GTAC	2 cut(s)	663, 933
RseI	CAYNNNNRTG	2 cut(s)	962, 1007
SacI	GAGCTC	1 cut(s)	534
SaqAI	TTAA	4 cut(s)	12, 165, 195, 254
SatI	GCNGC	6 cut(s)	20, 471, 577, 696, 981, 1014
Sau3AI	GATC	2 cut(s)	48, 510
Sau96I	GGNCC	4 cut(s)	63, 424, 742, 837
SchI	GAGTC	2 cut(s)	130, 172
ScrFI	CCNGG	2 cut(s)	67, 835
SduI	GDGCHC	2 cut(s)	332, 534
SfaNI	GCATC	7 cut(s)	91, 113, 223, 377, 434, 580, 778
SfcI	CTRYAG	1 cut(s)	17
SinI	GGWCC	2 cut(s)	424, 837
SmiMI	CAYNNNNRTG	2 cut(s)	962, 1007
SmlI	CTYRAG	2 cut(s)	40, 301
SmoI	CTYRAG	2 cut(s)	40, 301
Sse9I	AATT	5 cut(s)	266, 358, 483, 506, 637
SsiI	CCGC	8 cut(s)	438, 471, 549, 576, 618, 753, 870, 983
SspMI	CTAG	3 cut(s)	792, 819, 914
SstI	GAGCTC	1 cut(s)	534
StyD4I	CCNGG	2 cut(s)	65, 833
StyI	CCWWGG	3 cut(s)	427, 562, 859
TaaI	ACNGT	1 cut(s)	734
TaiI	ACGT	1 cut(s)	165
TaqI	TCGA	1 cut(s)	107
TasI	AATT	5 cut(s)	266, 358, 483, 506, 637
TauI	GCSGC	2 cut(s)	473, 579
TfiI	GAWTC	2 cut(s)	218, 312
Tru1I	TTAA	4 cut(s)	12, 165, 195, 254
Tru9I	TTAA	4 cut(s)	12, 165, 195, 254
TscAI	CASTG	2 cut(s)	471, 739
TseFI	GTSAC	1 cut(s)	542
TseI	GCWGC	4 cut(s)	19, 695, 980, 1013
Tsp45I	GTSAC	1 cut(s)	542
TspDTI	ATGAA	3 cut(s)	300, 369, 592
TspGWI	ACGGA	2 cut(s)	491, 950
TspRI	CASTG	2 cut(s)	471, 739
VpaK11BI	GGWCC	2 cut(s)	424, 837
XapI	RAATTY	1 cut(s)	358
XceI	RCATGY	1 cut(s)	669
XspI	CTAG	3 cut(s)	792, 819, 914
Zsp2I	ATGCAT	1 cut(s)	106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.