Belongs to the peptidase C1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 249 |
| Acc65I | GGTACC | 1 cut(s) | 893 |
| AccB1I | GGYRCC | 1 cut(s) | 893 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 903 |
| AccI | GTMKAC | 1 cut(s) | 552 |
| AciI | CCGC | 2 cut(s) | 789, 864 |
| AclWI | GGATC | 1 cut(s) | 838 |
| AcoI | YGGCCR | 1 cut(s) | 703 |
| AcsI | RAATTY | 1 cut(s) | 290 |
| AcyI | GRCGYC | 2 cut(s) | 410, 783 |
| AfaI | GTAC | 4 cut(s) | 20, 622, 658, 895 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 644, 745, 903 |
| AflII | CTTAAG | 1 cut(s) | 743 |
| AflIII | ACRYGT | 1 cut(s) | 148 |
| AgsI | TTSAA | 8 cut(s) | 167, 187, 196, 295, 352, 593, 803, 813 |
| AjiI | CACGTC | 1 cut(s) | 149 |
| AluBI | AGCT | 3 cut(s) | 404, 487, 734 |
| AluI | AGCT | 3 cut(s) | 404, 487, 734 |
| Alw26I | GTCTC | 1 cut(s) | 620 |
| AlwI | GGATC | 1 cut(s) | 838 |
| AoxI | GGCC | 3 cut(s) | 42, 427, 703 |
| ApeKI | GCWGC | 3 cut(s) | 464, 689, 734 |
| ApoI | RAATTY | 1 cut(s) | 290 |
| Asp718I | GGTACC | 1 cut(s) | 893 |
| AsuHPI | GGTGA | 2 cut(s) | 922, 950 |
| AsuNHI | GCTAGC | 1 cut(s) | 680 |
| BaeGI | GKGCMC | 1 cut(s) | 929 |
| BalI | TGGCCA | 1 cut(s) | 705 |
| BanI | GGYRCC | 1 cut(s) | 893 |
| BbsI | GAAGAC | 1 cut(s) | 63 |
| BbvI | GCAGC | 3 cut(s) | 476, 676, 721 |
| BccI | CCATC | 4 cut(s) | 127, 571, 647, 884 |
| BciVI | GTATCC | 1 cut(s) | 941 |
| BclI | TGATCA | 1 cut(s) | 850 |
| BcoDI | GTCTC | 1 cut(s) | 620 |
| BfaI | CTAG | 2 cut(s) | 303, 681 |
| BfmI | CTRYAG | 1 cut(s) | 378 |
| BfrI | CTTAAG | 1 cut(s) | 743 |
| BfuI | GTATCC | 1 cut(s) | 941 |
| BisI | GCNGC | 3 cut(s) | 465, 690, 735 |
| BlsI | GCNGC | 3 cut(s) | 466, 691, 736 |
| BmcAI | AGTACT | 1 cut(s) | 20 |
| BmgBI | CACGTC | 1 cut(s) | 149 |
| BmiI | GGNNCC | 1 cut(s) | 895 |
| BmsI | GCATC | 5 cut(s) | 91, 113, 168, 223, 574 |
| BmtI | GCTAGC | 1 cut(s) | 684 |
| BpiI | GAAGAC | 1 cut(s) | 63 |
| BpuEI | CTTGAG | 1 cut(s) | 60 |
| BsaHI | GRCGYC | 2 cut(s) | 410, 783 |
| BsaJI | CCNNGG | 3 cut(s) | 424, 468, 556 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 644, 745, 903 |
| Bse118I | RCCGGY | 1 cut(s) | 785 |
| Bse1I | ACTGG | 3 cut(s) | 16, 499, 706 |
| Bse3DI | GCAATG | 2 cut(s) | 134, 996 |
| BseDI | CCNNGG | 3 cut(s) | 424, 468, 556 |
| BseGI | GGATG | 4 cut(s) | 138, 221, 443, 586 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 644, 745, 903 |
| BseMI | GCAATG | 2 cut(s) | 134, 996 |
| BseMII | CTCAG | 1 cut(s) | 507 |
| BseNI | ACTGG | 3 cut(s) | 16, 499, 706 |
| BseSI | GKGCMC | 1 cut(s) | 929 |
| BseXI | GCAGC | 3 cut(s) | 476, 676, 721 |
| BshFI | GGCC | 3 cut(s) | 44, 429, 705 |
| BshNI | GGYRCC | 1 cut(s) | 893 |
| BsiSI | CCGG | 1 cut(s) | 786 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 644, 745, 903 |
| BsmAI | GTCTC | 1 cut(s) | 620 |
| BsmI | GAATGC | 3 cut(s) | 209, 332, 963 |
| BsnI | GGCC | 3 cut(s) | 44, 429, 705 |
| Bsp1286I | GDGCHC | 1 cut(s) | 929 |
| Bsp143I | GATC | 4 cut(s) | 48, 504, 830, 850 |
| Bsp19I | CCATGG | 1 cut(s) | 468 |
| BspACI | CCGC | 2 cut(s) | 789, 864 |
| BspANI | GGCC | 3 cut(s) | 44, 429, 705 |
| BspCNI | CTCAG | 1 cut(s) | 508 |
| BspLI | GGNNCC | 1 cut(s) | 895 |
| BspOI | GCTAGC | 1 cut(s) | 684 |
| BspPI | GGATC | 1 cut(s) | 838 |
| BspT107I | GGYRCC | 1 cut(s) | 893 |
| BspTI | CTTAAG | 1 cut(s) | 743 |
| BsrDI | GCAATG | 2 cut(s) | 134, 996 |
| BsrFI | RCCGGY | 1 cut(s) | 785 |
| BsrI | ACTGG | 3 cut(s) | 16, 499, 706 |
| BssAI | RCCGGY | 1 cut(s) | 785 |
| BssECI | CCNNGG | 3 cut(s) | 424, 468, 556 |
| BssMI | GATC | 4 cut(s) | 48, 504, 830, 850 |
| BssNI | GRCGYC | 2 cut(s) | 410, 783 |
| BssT1I | CCWWGG | 3 cut(s) | 424, 468, 556 |
| Bst4CI | ACNGT | 2 cut(s) | 382, 728 |
| BstACI | GRCGYC | 2 cut(s) | 410, 783 |
| BstAFI | CTTAAG | 1 cut(s) | 743 |
| BstC8I | GCNNGC | 4 cut(s) | 139, 682, 720, 787 |
| BstDEI | CTNAG | 4 cut(s) | 316, 483, 516, 617 |
| BstDSI | CCRYGG | 1 cut(s) | 468 |
| BstEII | GGTNACC | 1 cut(s) | 497 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 743 |
| BstF5I | GGATG | 4 cut(s) | 138, 221, 443, 586 |
| BstKTI | GATC | 4 cut(s) | 51, 507, 833, 853 |
| BstMAI | GTCTC | 1 cut(s) | 620 |
| BstMBI | GATC | 4 cut(s) | 48, 504, 830, 850 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 455, 464, 686, 782, 1007 |
| BstPI | GGTNACC | 1 cut(s) | 497 |
| BstSFI | CTRYAG | 1 cut(s) | 378 |
| BstSLI | GKGCMC | 1 cut(s) | 929 |
| BstV1I | GCAGC | 3 cut(s) | 476, 676, 721 |
| BstV2I | GAAGAC | 1 cut(s) | 63 |
| BstX2I | RGATCY | 1 cut(s) | 830 |
| BstYI | RGATCY | 1 cut(s) | 830 |
| BsuI | GTATCC | 1 cut(s) | 941 |
| BsuRI | GGCC | 3 cut(s) | 44, 429, 705 |
| BtgI | CCRYGG | 1 cut(s) | 468 |
| BtgZI | GCGATG | 1 cut(s) | 246 |
| BtrI | CACGTC | 1 cut(s) | 149 |
| BtsCI | GGATG | 4 cut(s) | 138, 221, 443, 586 |
| BtsI | GCAGTG | 2 cut(s) | 465, 1020 |
| BtsIMutI | CAGTG | 4 cut(s) | 465, 733, 889, 1020 |
| Cac8I | GCNNGC | 4 cut(s) | 139, 682, 720, 787 |
| Cfr10I | RCCGGY | 1 cut(s) | 785 |
| CseI | GACGC | 2 cut(s) | 418, 791 |
| Csp6I | GTAC | 4 cut(s) | 19, 621, 657, 894 |
| CviAII | CATG | 6 cut(s) | 55, 323, 469, 611, 854, 921 |
| CviQI | GTAC | 4 cut(s) | 19, 621, 657, 894 |
| DdeI | CTNAG | 4 cut(s) | 316, 483, 516, 617 |
| DpnI | GATC | 4 cut(s) | 50, 506, 832, 852 |
| DpnII | GATC | 4 cut(s) | 48, 504, 830, 850 |
| EaeI | YGGCCR | 1 cut(s) | 703 |
| Eco130I | CCWWGG | 3 cut(s) | 424, 468, 556 |
| Eco91I | GGTNACC | 1 cut(s) | 497 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 743 |
| EcoO65I | GGTNACC | 1 cut(s) | 497 |
| EcoRI | GAATTC | 1 cut(s) | 290 |
| EcoT14I | CCWWGG | 3 cut(s) | 424, 468, 556 |
| EcoT22I | ATGCAT | 2 cut(s) | 106, 589 |
| ErhI | CCWWGG | 3 cut(s) | 424, 468, 556 |
| FaeI | CATG | 6 cut(s) | 58, 326, 472, 614, 857, 924 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 723, 755, 972, 1004 |
| FatI | CATG | 6 cut(s) | 54, 322, 468, 610, 853, 920 |
| FbaI | TGATCA | 1 cut(s) | 850 |
| FblI | GTMKAC | 1 cut(s) | 552 |
| Fnu4HI | GCNGC | 3 cut(s) | 465, 690, 735 |
| FokI | GGATG | 4 cut(s) | 145, 208, 450, 593 |
| Fsp4HI | GCNGC | 3 cut(s) | 465, 690, 735 |
| FspBI | CTAG | 2 cut(s) | 303, 681 |
| GluI | GCNGC | 3 cut(s) | 465, 690, 735 |
| HaeIII | GGCC | 3 cut(s) | 44, 429, 705 |
| HapII | CCGG | 1 cut(s) | 786 |
| HgaI | GACGC | 2 cut(s) | 418, 791 |
| Hin1I | GRCGYC | 2 cut(s) | 410, 783 |
| Hin1II | CATG | 6 cut(s) | 58, 326, 472, 614, 857, 924 |
| HindIII | AAGCTT | 1 cut(s) | 485 |
| HinfI | GANTC | 3 cut(s) | 218, 312, 879 |
| HpaII | CCGG | 1 cut(s) | 786 |
| HphI | GGTGA | 2 cut(s) | 922, 950 |
| Hpy166II | GTNNAC | 1 cut(s) | 553 |
| Hpy188I | TCNGA | 2 cut(s) | 517, 796 |
| Hpy188III | TCNNGA | 4 cut(s) | 167, 187, 303, 848 |
| Hpy8I | GTNNAC | 1 cut(s) | 553 |
| HpyAV | CCTTC | 3 cut(s) | 467, 749, 977 |
| HpyCH4III | ACNGT | 2 cut(s) | 382, 728 |
| HpyCH4IV | ACGT | 1 cut(s) | 148 |
| HpyCH4V | TGCA | 8 cut(s) | 27, 104, 236, 587, 663, 722, 961, 1025 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 455, 464, 686, 782, 1007 |
| HpyF3I | CTNAG | 4 cut(s) | 316, 483, 516, 617 |
| HpySE526I | ACGT | 1 cut(s) | 148 |
| Hsp92I | GRCGYC | 2 cut(s) | 410, 783 |
| Hsp92II | CATG | 6 cut(s) | 58, 326, 472, 614, 857, 924 |
| KpnI | GGTACC | 1 cut(s) | 897 |
| KroI | GCCGGC | 1 cut(s) | 785 |
| KroNI | GCCGGC | 1 cut(s) | 787 |
| Ksp22I | TGATCA | 1 cut(s) | 850 |
| Kzo9I | GATC | 4 cut(s) | 48, 504, 830, 850 |
| LmnI | GCTCC | 2 cut(s) | 335, 401 |
| LpnPI | CCDG | 8 cut(s) | 29, 480, 515, 630, 687, 732, 799, 813 |
| Lsp1109I | GCAGC | 3 cut(s) | 476, 676, 721 |
| LweI | GCATC | 5 cut(s) | 91, 113, 168, 223, 574 |
| MaeI | CTAG | 2 cut(s) | 303, 681 |
| MaeII | ACGT | 1 cut(s) | 148 |
| MaeIII | GTNAC | 5 cut(s) | 364, 406, 497, 536, 859 |
| MalI | GATC | 4 cut(s) | 50, 506, 832, 852 |
| MboI | GATC | 4 cut(s) | 48, 504, 830, 850 |
| MboII | GAAGA | 5 cut(s) | 43, 63, 299, 722, 925 |
| MflI | RGATCY | 1 cut(s) | 830 |
| MhlI | GDGCHC | 1 cut(s) | 929 |
| MlsI | TGGCCA | 1 cut(s) | 705 |
| MluCI | AATT | 7 cut(s) | 251, 266, 290, 477, 593, 631, 803 |
| MluNI | TGGCCA | 1 cut(s) | 705 |
| MlyI | GAGTC | 1 cut(s) | 888 |
| MnlI | CCTC | 6 cut(s) | 310, 559, 670, 959, 967, 1021 |
| Mox20I | TGGCCA | 1 cut(s) | 705 |
| Mph1103I | ATGCAT | 2 cut(s) | 106, 589 |
| MreI | CGCCGGCG | 1 cut(s) | 785 |
| MroNI | GCCGGC | 1 cut(s) | 785 |
| MscI | TGGCCA | 1 cut(s) | 705 |
| MseI | TTAA | 2 cut(s) | 12, 744 |
| MslI | CAYNNNNRTG | 3 cut(s) | 711, 956, 1001 |
| Msp20I | TGGCCA | 1 cut(s) | 705 |
| MspA1I | CMGCKG | 1 cut(s) | 866 |
| MspCI | CTTAAG | 1 cut(s) | 743 |
| MspI | CCGG | 1 cut(s) | 786 |
| Mva1269I | GAATGC | 3 cut(s) | 209, 332, 963 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 455, 464, 686, 782, 1007 |
| NaeI | GCCGGC | 1 cut(s) | 787 |
| NcoI | CCATGG | 1 cut(s) | 468 |
| NdeII | GATC | 4 cut(s) | 48, 504, 830, 850 |
| NgoMIV | GCCGGC | 1 cut(s) | 785 |
| NheI | GCTAGC | 1 cut(s) | 680 |
| NlaIII | CATG | 6 cut(s) | 58, 326, 472, 614, 857, 924 |
| NlaIV | GGNNCC | 1 cut(s) | 895 |
| NmuCI | GTSAC | 2 cut(s) | 406, 536 |
| NsiI | ATGCAT | 2 cut(s) | 106, 589 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 145 |
| PctI | GAATGC | 3 cut(s) | 209, 332, 963 |
| PdiI | GCCGGC | 1 cut(s) | 787 |
| PfeI | GAWTC | 2 cut(s) | 218, 312 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 903 |
| PkrI | GCNGC | 3 cut(s) | 466, 691, 736 |
| PleI | GAGTC | 1 cut(s) | 887 |
| PpsI | GAGTC | 1 cut(s) | 887 |
| PsiI | TTATAA | 1 cut(s) | 249 |
| PspEI | GGTNACC | 1 cut(s) | 497 |
| PspN4I | GGNNCC | 1 cut(s) | 895 |
| PsuI | RGATCY | 1 cut(s) | 830 |
| RsaI | GTAC | 4 cut(s) | 20, 622, 658, 895 |
| RsaNI | GTAC | 4 cut(s) | 19, 621, 657, 894 |
| RseI | CAYNNNNRTG | 3 cut(s) | 711, 956, 1001 |
| SaqAI | TTAA | 2 cut(s) | 12, 744 |
| SatI | GCNGC | 3 cut(s) | 465, 690, 735 |
| Sau3AI | GATC | 4 cut(s) | 48, 504, 830, 850 |
| ScaI | AGTACT | 1 cut(s) | 20 |
| SchI | GAGTC | 1 cut(s) | 888 |
| SduI | GDGCHC | 1 cut(s) | 929 |
| SfaNI | GCATC | 5 cut(s) | 91, 113, 168, 223, 574 |
| SfcI | CTRYAG | 1 cut(s) | 378 |
| SgrAI | CRCCGGYG | 1 cut(s) | 785 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 711, 956, 1001 |
| SmlI | CTYRAG | 2 cut(s) | 75, 743 |
| SmoI | CTYRAG | 2 cut(s) | 75, 743 |
| Sse9I | AATT | 7 cut(s) | 251, 266, 290, 477, 593, 631, 803 |
| SsiI | CCGC | 2 cut(s) | 789, 864 |
| SspMI | CTAG | 2 cut(s) | 303, 681 |
| StyI | CCWWGG | 3 cut(s) | 424, 468, 556 |
| TaaI | ACNGT | 2 cut(s) | 382, 728 |
| TaiI | ACGT | 1 cut(s) | 151 |
| TasI | AATT | 7 cut(s) | 251, 266, 290, 477, 593, 631, 803 |
| TatI | WGTACW | 1 cut(s) | 18 |
| TfiI | GAWTC | 2 cut(s) | 218, 312 |
| Tru1I | TTAA | 2 cut(s) | 12, 744 |
| Tru9I | TTAA | 2 cut(s) | 12, 744 |
| TscAI | CASTG | 4 cut(s) | 465, 733, 889, 1027 |
| TseFI | GTSAC | 2 cut(s) | 406, 536 |
| TseI | GCWGC | 3 cut(s) | 464, 689, 734 |
| Tsp45I | GTSAC | 2 cut(s) | 406, 536 |
| TspDTI | ATGAA | 5 cut(s) | 43, 202, 339, 372, 586 |
| TspRI | CASTG | 4 cut(s) | 465, 733, 889, 1027 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 903 |
| Vha464I | CTTAAG | 1 cut(s) | 743 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 743 |
| XapI | RAATTY | 1 cut(s) | 290 |
| XbaI | TCTAGA | 1 cut(s) | 302 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 431, 904 |
| XmiI | GTMKAC | 1 cut(s) | 552 |
| XspI | CTAG | 2 cut(s) | 303, 681 |
| ZrmI | AGTACT | 1 cut(s) | 20 |
| Zsp2I | ATGCAT | 2 cut(s) | 106, 589 |