Belongs to the peptidase C1 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 545 |
| AciI | CCGC | 2 cut(s) | 338, 867 |
| AclWI | GGATC | 3 cut(s) | 43, 435, 905 |
| AcsI | RAATTY | 1 cut(s) | 290 |
| AcuI | CTGAAG | 4 cut(s) | 96, 306, 473, 678 |
| AcyI | GRCGYC | 1 cut(s) | 148 |
| AfaI | GTAC | 2 cut(s) | 359, 602 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 87, 751 |
| AgsI | TTSAA | 9 cut(s) | 98, 187, 196, 295, 316, 355, 387, 788, 965 |
| AhlI | ACTAGT | 1 cut(s) | 508 |
| AjnI | CCWGG | 1 cut(s) | 15 |
| AluBI | AGCT | 5 cut(s) | 410, 447, 485, 493, 791 |
| AluI | AGCT | 5 cut(s) | 410, 447, 485, 493, 791 |
| Alw21I | GWGCWC | 2 cut(s) | 24, 487 |
| Alw26I | GTCTC | 2 cut(s) | 170, 517 |
| Alw44I | GTGCAC | 1 cut(s) | 20 |
| AlwI | GGATC | 3 cut(s) | 43, 435, 905 |
| AoxI | GGCC | 1 cut(s) | 433 |
| ApaLI | GTGCAC | 1 cut(s) | 20 |
| ApeKI | GCWGC | 2 cut(s) | 104, 159 |
| ApoI | RAATTY | 1 cut(s) | 290 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 243, 275 |
| AspS9I | GGNCC | 2 cut(s) | 742, 847 |
| AsuHPI | GGTGA | 3 cut(s) | 515, 566, 953 |
| AsuII | TTCGAA | 2 cut(s) | 224, 804 |
| AvaII | GGWCC | 2 cut(s) | 742, 847 |
| BaeGI | GKGCMC | 2 cut(s) | 24, 932 |
| BanII | GRGCYC | 1 cut(s) | 487 |
| BbsI | GAAGAC | 2 cut(s) | 63, 314 |
| Bbv12I | GWGCWC | 2 cut(s) | 24, 487 |
| BbvCI | CCTCAGC | 1 cut(s) | 792 |
| BbvI | GCAGC | 2 cut(s) | 116, 171 |
| BccI | CCATC | 1 cut(s) | 388 |
| BciT130I | CCWGG | 1 cut(s) | 17 |
| BciVI | GTATCC | 2 cut(s) | 322, 784 |
| BcoDI | GTCTC | 2 cut(s) | 170, 517 |
| BcuI | ACTAGT | 1 cut(s) | 508 |
| BfaI | CTAG | 1 cut(s) | 509 |
| BfuI | GTATCC | 2 cut(s) | 322, 784 |
| BisI | GCNGC | 2 cut(s) | 105, 160 |
| BlsI | GCNGC | 2 cut(s) | 106, 161 |
| Bme1390I | CCNGG | 1 cut(s) | 17 |
| Bme18I | GGWCC | 2 cut(s) | 742, 847 |
| BmgT120I | GGNCC | 2 cut(s) | 742, 847 |
| BmiI | GGNNCC | 1 cut(s) | 472 |
| BmrFI | CCNGG | 1 cut(s) | 17 |
| BmsI | GCATC | 1 cut(s) | 91 |
| BpiI | GAAGAC | 2 cut(s) | 63, 314 |
| BpmI | CTGGAG | 1 cut(s) | 415 |
| Bpu10I | CCTNAGC | 1 cut(s) | 792 |
| Bpu14I | TTCGAA | 2 cut(s) | 224, 804 |
| BpuEI | CTTGAG | 1 cut(s) | 734 |
| Bsa29I | ATCGAT | 1 cut(s) | 900 |
| BsaHI | GRCGYC | 1 cut(s) | 148 |
| BsaJI | CCNNGG | 1 cut(s) | 474 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 87, 751 |
| Bse118I | RCCGGY | 1 cut(s) | 707 |
| Bse1I | ACTGG | 3 cut(s) | 313, 398, 505 |
| Bse3DI | GCAATG | 3 cut(s) | 134, 216, 999 |
| BseBI | CCWGG | 1 cut(s) | 17 |
| BseCI | ATCGAT | 1 cut(s) | 900 |
| BseDI | CCNNGG | 1 cut(s) | 474 |
| BseGI | GGATG | 1 cut(s) | 138 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 87, 751 |
| BseMI | GCAATG | 3 cut(s) | 134, 216, 999 |
| BseMII | CTCAG | 1 cut(s) | 783 |
| BseNI | ACTGG | 3 cut(s) | 313, 398, 505 |
| BseSI | GKGCMC | 2 cut(s) | 24, 932 |
| BseXI | GCAGC | 2 cut(s) | 116, 171 |
| BshFI | GGCC | 1 cut(s) | 435 |
| BshVI | ATCGAT | 1 cut(s) | 900 |
| BsiHKAI | GWGCWC | 2 cut(s) | 24, 487 |
| BsiSI | CCGG | 1 cut(s) | 708 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 87, 751 |
| BsmAI | GTCTC | 2 cut(s) | 170, 517 |
| BsmI | GAATGC | 2 cut(s) | 783, 800 |
| BsnI | GGCC | 1 cut(s) | 435 |
| Bsp119I | TTCGAA | 2 cut(s) | 224, 804 |
| Bsp1286I | GDGCHC | 3 cut(s) | 24, 487, 932 |
| Bsp1407I | TGTACA | 1 cut(s) | 600 |
| Bsp143I | GATC | 3 cut(s) | 48, 427, 897 |
| BspACI | CCGC | 2 cut(s) | 338, 867 |
| BspANI | GGCC | 1 cut(s) | 435 |
| BspCNI | CTCAG | 1 cut(s) | 784 |
| BspDI | ATCGAT | 1 cut(s) | 900 |
| BspLI | GGNNCC | 1 cut(s) | 472 |
| BspPI | GGATC | 3 cut(s) | 43, 435, 905 |
| BspQI | GCTCTTC | 1 cut(s) | 492 |
| BspT104I | TTCGAA | 2 cut(s) | 224, 804 |
| BsrDI | GCAATG | 3 cut(s) | 134, 216, 999 |
| BsrFI | RCCGGY | 1 cut(s) | 707 |
| BsrGI | TGTACA | 1 cut(s) | 600 |
| BsrI | ACTGG | 3 cut(s) | 313, 398, 505 |
| BssAI | RCCGGY | 1 cut(s) | 707 |
| BssECI | CCNNGG | 1 cut(s) | 474 |
| BssMI | GATC | 3 cut(s) | 48, 427, 897 |
| BssNI | GRCGYC | 1 cut(s) | 148 |
| BssT1I | CCWWGG | 1 cut(s) | 474 |
| Bst2UI | CCWGG | 1 cut(s) | 17 |
| Bst4CI | ACNGT | 4 cut(s) | 466, 542, 734, 1027 |
| Bst6I | CTCTTC | 1 cut(s) | 492 |
| BstACI | GRCGYC | 1 cut(s) | 148 |
| BstAUI | TGTACA | 1 cut(s) | 600 |
| BstBI | TTCGAA | 2 cut(s) | 224, 804 |
| BstC8I | GCNNGC | 1 cut(s) | 495 |
| BstDEI | CTNAG | 1 cut(s) | 792 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 749 |
| BstF5I | GGATG | 1 cut(s) | 138 |
| BstKTI | GATC | 3 cut(s) | 51, 430, 900 |
| BstMAI | GTCTC | 2 cut(s) | 170, 517 |
| BstMBI | GATC | 3 cut(s) | 48, 427, 897 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 156, 708, 797, 1010, 1019 |
| BstNI | CCWGG | 1 cut(s) | 17 |
| BstSCI | CCNGG | 1 cut(s) | 15 |
| BstSLI | GKGCMC | 2 cut(s) | 24, 932 |
| BstV1I | GCAGC | 2 cut(s) | 116, 171 |
| BstV2I | GAAGAC | 2 cut(s) | 63, 314 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 308 |
| Bsu15I | ATCGAT | 1 cut(s) | 900 |
| BsuI | GTATCC | 2 cut(s) | 322, 784 |
| BsuRI | GGCC | 1 cut(s) | 435 |
| BsuTUI | ATCGAT | 1 cut(s) | 900 |
| BtgZI | GCGATG | 1 cut(s) | 246 |
| BtsCI | GGATG | 1 cut(s) | 138 |
| BtsIMutI | CAGTG | 2 cut(s) | 471, 739 |
| Cac8I | GCNNGC | 1 cut(s) | 495 |
| Cfr10I | RCCGGY | 1 cut(s) | 707 |
| Cfr13I | GGNCC | 2 cut(s) | 742, 847 |
| ClaI | ATCGAT | 1 cut(s) | 900 |
| CseI | GACGC | 2 cut(s) | 156, 247 |
| CsiI | ACCWGGT | 1 cut(s) | 15 |
| Csp6I | GTAC | 2 cut(s) | 358, 601 |
| CviAII | CATG | 3 cut(s) | 109, 163, 924 |
| CviQI | GTAC | 2 cut(s) | 358, 601 |
| DdeI | CTNAG | 1 cut(s) | 792 |
| DpnI | GATC | 3 cut(s) | 50, 429, 899 |
| DpnII | GATC | 3 cut(s) | 48, 427, 897 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 545 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 545 |
| Eam1104I | CTCTTC | 1 cut(s) | 492 |
| EarI | CTCTTC | 1 cut(s) | 492 |
| EciI | GGCGGA | 1 cut(s) | 327 |
| Ecl136II | GAGCTC | 1 cut(s) | 485 |
| Eco130I | CCWWGG | 1 cut(s) | 474 |
| Eco24I | GRGCYC | 1 cut(s) | 487 |
| Eco47I | GGWCC | 2 cut(s) | 742, 847 |
| Eco53kI | GAGCTC | 1 cut(s) | 485 |
| Eco57I | CTGAAG | 4 cut(s) | 96, 306, 473, 678 |
| EcoICRI | GAGCTC | 1 cut(s) | 485 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 749 |
| EcoO109I | RGGNCCY | 1 cut(s) | 742 |
| EcoRI | GAATTC | 1 cut(s) | 290 |
| EcoRII | CCWGG | 1 cut(s) | 15 |
| EcoT14I | CCWWGG | 1 cut(s) | 474 |
| EcoT38I | GRGCYC | 1 cut(s) | 487 |
| ErhI | CCWWGG | 1 cut(s) | 474 |
| FaeI | CATG | 3 cut(s) | 112, 166, 927 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 471, 503 |
| FatI | CATG | 3 cut(s) | 108, 162, 923 |
| Fnu4HI | GCNGC | 2 cut(s) | 105, 160 |
| FokI | GGATG | 1 cut(s) | 145 |
| FriOI | GRGCYC | 1 cut(s) | 487 |
| Fsp4HI | GCNGC | 2 cut(s) | 105, 160 |
| FspBI | CTAG | 1 cut(s) | 509 |
| GluI | GCNGC | 2 cut(s) | 105, 160 |
| GsuI | CTGGAG | 1 cut(s) | 415 |
| HaeIII | GGCC | 1 cut(s) | 435 |
| HapII | CCGG | 1 cut(s) | 708 |
| HgaI | GACGC | 2 cut(s) | 156, 247 |
| Hin1I | GRCGYC | 1 cut(s) | 148 |
| Hin1II | CATG | 3 cut(s) | 112, 166, 927 |
| HincII | GTYRAC | 2 cut(s) | 538, 828 |
| HindII | GTYRAC | 2 cut(s) | 538, 828 |
| HinfI | GANTC | 6 cut(s) | 91, 136, 178, 218, 312, 961 |
| HpaII | CCGG | 1 cut(s) | 708 |
| HphI | GGTGA | 3 cut(s) | 515, 566, 953 |
| Hpy166II | GTNNAC | 7 cut(s) | 22, 238, 506, 538, 730, 737, 828 |
| Hpy188I | TCNGA | 4 cut(s) | 76, 90, 523, 551 |
| Hpy188III | TCNNGA | 3 cut(s) | 95, 196, 326 |
| Hpy8I | GTNNAC | 7 cut(s) | 22, 238, 506, 538, 730, 737, 828 |
| Hpy99I | CGWCG | 2 cut(s) | 551, 590 |
| HpyAV | CCTTC | 7 cut(s) | 166, 473, 557, 702, 755, 941, 980 |
| HpyCH4III | ACNGT | 4 cut(s) | 466, 542, 734, 1027 |
| HpyCH4IV | ACGT | 4 cut(s) | 546, 588, 954, 1007 |
| HpyCH4V | TGCA | 5 cut(s) | 22, 104, 272, 669, 783 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 156, 708, 797, 1010, 1019 |
| HpyF3I | CTNAG | 1 cut(s) | 792 |
| HpySE526I | ACGT | 4 cut(s) | 546, 588, 954, 1007 |
| Hsp92I | GRCGYC | 1 cut(s) | 148 |
| Hsp92II | CATG | 3 cut(s) | 112, 166, 927 |
| Kzo9I | GATC | 3 cut(s) | 48, 427, 897 |
| LguI | GCTCTTC | 1 cut(s) | 492 |
| LmnI | GCTCC | 4 cut(s) | 407, 470, 482, 797 |
| Lsp1109I | GCAGC | 2 cut(s) | 116, 171 |
| LweI | GCATC | 1 cut(s) | 91 |
| MabI | ACCWGGT | 1 cut(s) | 15 |
| MaeI | CTAG | 1 cut(s) | 509 |
| MaeII | ACGT | 4 cut(s) | 546, 588, 954, 1007 |
| MaeIII | GTNAC | 3 cut(s) | 542, 862, 875 |
| MalI | GATC | 3 cut(s) | 50, 429, 899 |
| MboI | GATC | 3 cut(s) | 48, 427, 897 |
| MboII | GAAGA | 9 cut(s) | 63, 110, 213, 299, 314, 375, 479, 728, 956 |
| MhlI | GDGCHC | 3 cut(s) | 24, 487, 932 |
| MluCI | AATT | 4 cut(s) | 251, 266, 290, 637 |
| MlyI | GAGTC | 2 cut(s) | 130, 172 |
| MmeI | TCCRAC | 1 cut(s) | 529 |
| MspI | CCGG | 1 cut(s) | 708 |
| MspR9I | CCNGG | 1 cut(s) | 17 |
| Mva1269I | GAATGC | 2 cut(s) | 783, 800 |
| MvaI | CCWGG | 1 cut(s) | 17 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 156, 708, 797, 1010, 1019 |
| NdeII | GATC | 3 cut(s) | 48, 427, 897 |
| NlaIII | CATG | 3 cut(s) | 112, 166, 927 |
| NlaIV | GGNNCC | 1 cut(s) | 472 |
| NspV | TTCGAA | 2 cut(s) | 224, 804 |
| PciSI | GCTCTTC | 1 cut(s) | 492 |
| PctI | GAATGC | 2 cut(s) | 783, 800 |
| PfeI | GAWTC | 4 cut(s) | 91, 218, 312, 961 |
| PkrI | GCNGC | 2 cut(s) | 106, 161 |
| PleI | GAGTC | 2 cut(s) | 130, 172 |
| PpsI | GAGTC | 2 cut(s) | 130, 172 |
| PpuMI | RGGWCCY | 1 cut(s) | 742 |
| Psp124BI | GAGCTC | 1 cut(s) | 487 |
| Psp5II | RGGWCCY | 1 cut(s) | 742 |
| Psp6I | CCWGG | 1 cut(s) | 15 |
| PspGI | CCWGG | 1 cut(s) | 15 |
| PspN4I | GGNNCC | 1 cut(s) | 472 |
| PspPI | GGNCC | 2 cut(s) | 742, 847 |
| PspPPI | RGGWCCY | 1 cut(s) | 742 |
| RsaI | GTAC | 2 cut(s) | 359, 602 |
| RsaNI | GTAC | 2 cut(s) | 358, 601 |
| SacI | GAGCTC | 1 cut(s) | 487 |
| SapI | GCTCTTC | 1 cut(s) | 492 |
| SatI | GCNGC | 2 cut(s) | 105, 160 |
| Sau3AI | GATC | 3 cut(s) | 48, 427, 897 |
| Sau96I | GGNCC | 2 cut(s) | 742, 847 |
| SchI | GAGTC | 2 cut(s) | 130, 172 |
| ScrFI | CCNGG | 1 cut(s) | 17 |
| SduI | GDGCHC | 3 cut(s) | 24, 487, 932 |
| SexAI | ACCWGGT | 1 cut(s) | 15 |
| SfaNI | GCATC | 1 cut(s) | 91 |
| SfuI | TTCGAA | 2 cut(s) | 224, 804 |
| SinI | GGWCC | 2 cut(s) | 742, 847 |
| SmlI | CTYRAG | 1 cut(s) | 749 |
| SmoI | CTYRAG | 1 cut(s) | 749 |
| SpeI | ACTAGT | 1 cut(s) | 508 |
| Sse9I | AATT | 4 cut(s) | 251, 266, 290, 637 |
| SsiI | CCGC | 2 cut(s) | 338, 867 |
| SspMI | CTAG | 1 cut(s) | 509 |
| SstI | GAGCTC | 1 cut(s) | 487 |
| StyD4I | CCNGG | 1 cut(s) | 15 |
| StyI | CCWWGG | 1 cut(s) | 474 |
| TaaI | ACNGT | 4 cut(s) | 466, 542, 734, 1027 |
| TaiI | ACGT | 4 cut(s) | 549, 591, 957, 1010 |
| TaqI | TCGA | 4 cut(s) | 224, 804, 852, 900 |
| TasI | AATT | 4 cut(s) | 251, 266, 290, 637 |
| TatI | WGTACW | 1 cut(s) | 600 |
| TfiI | GAWTC | 4 cut(s) | 91, 218, 312, 961 |
| TscAI | CASTG | 2 cut(s) | 471, 739 |
| TseI | GCWGC | 2 cut(s) | 104, 159 |
| TspDTI | ATGAA | 3 cut(s) | 179, 576, 804 |
| TspGWI | ACGGA | 3 cut(s) | 766, 860, 894 |
| TspRI | CASTG | 2 cut(s) | 471, 739 |
| VneI | GTGCAC | 1 cut(s) | 20 |
| VpaK11BI | GGWCC | 2 cut(s) | 742, 847 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 749 |
| XapI | RAATTY | 1 cut(s) | 290 |
| XspI | CTAG | 1 cut(s) | 509 |