Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000141

Physical Location & Seq

Genomic Coordinates Forward (+)

28078019 .. 28081973

Gene Structure 3955 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1232400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 954 bp

ATGGAGATCCTAAACCTGCAGCTATGGAAGTATATGTGCTTGGGCTTGATCCTCATGTTGGGAGCTTGGTCCTCTGAAGCCACTTCTCGCATATATAACGACATCAATGAGAAGGAAACTCGTTTCAAGATATTCAAGGAAAATGTGGCGTTTATAGAATTTTCCAATAAAGATGCAAATAAACCTTACAAATTAAGTGTCAACCAATTTGCTGACCTTACAAATGAAGAGTTCAAAGCCACAAGAAATGGATTCAAGGGCCATGAGTGTTCCACAAAGGCTTCTTCTTTCAAATATGAAAATGTGACCGTACCATTACCAGCAACAATGGATTGGAGAAAGAAAGGAGCTGTAACCCCCGTTAAGGACCAAGGCCAGTGCGGATGTTGTTGGGCTTTTTCAGCAGTAGCCGCCACCGAAGGAATTACACAGCTTACAACTGGTAACTTGATCTCTTTGTCTGAGCAAGAGCTCGTTGATTGTGACACTGCTGGGGAAGACCAAGGTTGTGAGGGTGGCTTTATGGACGATGCGTTCCAGTTCATCCAACAAAATCACGGGATTAGCACAGAAACTAATTACCCCTACAACGGTGTTGATGGTACATGTAACACCAAGAAGGAAGCCATCATTGCAGCCAAGATAACTGGCTTTGAGGACGTGCCGGCAAATAGTGAAAAGGCCCTTCTAACCGCTGTTGCTCATCAACCTGTTTCCGTTGCCATCGATGCTAGCGGTTCCGACTTCCAATTCTATTCAAGTGGTGTCTTCACCGGAACTTGTGGAACGAGTCTAGACCATGGTGTTACCGCTGTTGGATATGGCGTGAGTGAGGATGGGACTAAGTATTGGCTAGTGAAGAACTCATGGGGTGCAGAATGGGGCGAAGCTGGGTACATAAGAATGCAAAGAGATGTTGCTGCAGCTGAAGGACTTTGTGGGATTGCTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

318

Amino Acids

34.53

Weight (kDa)

4.77

Isoelectric Point (pI)

20.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	29 - 78	1.1e-14	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	106 - 316	7.4e-83	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000141)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22160
fragaria_vesca	FvH4_1g23580 FvH4_1g25982 FvH4_2g17070 FvH4_3g40500 FvH4_6g27180 FvH4_6g27320 FvH4_6g33300 FvH4_6g33310 FvH4_7g09480 FvH4_7g13000 FvH4_7g13320 FvH4_7g13600
malus_domestica	MD00G1208000.v1.1 MD09G1238200.v1.1 MD09G1238600.v1.1 MD09G1238800.v1.1 MD09G1239100.v1.1 MD16G1226900.v1.1 MD16G1242700.v1.1 MD16G1244200.v1.1 MD16G1245500.v1.1 MD16G1245800.v1.1 MD17G1231600.v1.1 MD17G1231700.v1.1 MD17G1231800.v1.1 MD17G1232200.v1.1 MD17G1232300.v1.1 MD17G1232400.v1.1
prunus_persica	Prupe.1G072300_v2.0.a1 Prupe.1G072400_v2.0.a1 Prupe.3G106300_v2.0.a1 Prupe.3G106400_v2.0.a1 Prupe.3G119400_v2.0.a1 Prupe.3G120600_v2.0.a1 Prupe.3G121900_v2.0.a1 Prupe.3G122000_v2.0.a1 Prupe.3G122100_v2.0.a1 Prupe.3G122200_v2.0.a1 Prupe.3G122700_v2.0.a1 Prupe.5G055900_v2.0.a1 Prupe.5G056500_v2.0.a1
pyrus_communis	pycom09g15770 pycom09g15780 pycom09g15790 pycom16g20640 pycom17g18390
rosa_chinensis	RchiOBHm_Chr1g0352141 RchiOBHm_Chr1g0352581 RchiOBHm_Chr1g0352601 RchiOBHm_Chr1g0352621 RchiOBHm_Chr1g0352631 RchiOBHm_Chr2g0119131 RchiOBHm_Chr2g0128691 RchiOBHm_Chr2g0128701 RchiOBHm_Chr2g0129151 RchiOBHm_Chr2g0129221 RchiOBHm_Chr2g0129401 RchiOBHm_Chr2g0129421 RchiOBHm_Chr2g0129431 RchiOBHm_Chr2g0129471 RchiOBHm_Chr2g0129481 RchiOBHm_Chr2g0129491 RchiOBHm_Chr2g0143051 RchiOBHm_Chr2g0143121 RchiOBHm_Chr2g0143171 RchiOBHm_Chr3g0457341 RchiOBHm_Chr5g0049011 RchiOBHm_Chr6g0281281 RchiOBHm_Chr6g0281311
rosa_laevigata	RLG00000012975 RLG00000012976 RLG00000012979 RLG00000018447 RLG00000019018 RLG00000019061 RLG00000019066 RLG00000019078 RLG00000019079 RLG00000019080 RLG00000019083 RLG00000019085 RLG00000020009 RLG00000020010 RLG00000025234 RLG00000028326 RLG00000028327 RLG00000028328 RLG00000028329 RLG00000028357 RLG00000031784
rosa_multiflora	Rmu_co8169188.1_g000001 Rmu_co8261007.1_g000001 Rmu_co8314361.1_g000001 Rmu_sc0000109.1_g000014 Rmu_sc0000638.1_g000020 Rmu_sc0000847.1_g000020 Rmu_sc0000847.1_g000028 Rmu_sc0000847.1_g000037 Rmu_sc0000847.1_g000046 Rmu_sc0000911.1_g000034 Rmu_sc0000999.1_g000021 Rmu_sc0001171.1_g000013 Rmu_sc0001171.1_g000018 Rmu_sc0001171.1_g000019 Rmu_sc0001653.1_g000004 Rmu_sc0002277.1_g000005 Rmu_sc0003479.1_g000001 Rmu_sc0005118.1_g000006 Rmu_sc0005118.1_g000008 Rmu_sc0005227.1_g000012 Rmu_sc0005442.1_g000010 Rmu_sc0005514.1_g000005 Rmu_sc0005996.1_g000014 Rmu_sc0008800.1_g000002 Rmu_sc0008800.1_g000010 Rmu_sc0008800.1_g000014
rosa_roxburghii	Rroxscaffold_1G00065560 Rroxscaffold_2G00102080 Rroxscaffold_2G00102090 Rroxscaffold_2G00102110 Rroxscaffold_2G00114560 Rroxscaffold_2G00114570 Rroxscaffold_2G00114610 Rroxscaffold_2G00114620 Rroxscaffold_2G00114640 Rroxscaffold_2G00114740 Rroxscaffold_2G00114790 Rroxscaffold_2G00115860 Rroxscaffold_2G00124490 Rroxscaffold_4G00302260 Rroxscaffold_4G00302530 Rroxscaffold_4G00302550 Rroxscaffold_4G00302560 Rroxscaffold_4G00302620 Rroxscaffold_6G00421980 Rroxscaffold_7G00187140 Rroxscaffold_7G00187170
rosa_rugosa	Rorug01G0225000 Rorug01G0230200 Rorug02G0220500 Rorug02G0280000 Rorug02G0285200 Rorug02G0285400 Rorug02G0286300 Rorug02G0286300 Rorug02G0286400 Rorug02G0286900 Rorug02G0377800 Rorug02G0377900 Rorug02G0378000 Rorug02G0378000 Rorug02G0378800 Rorug03G0019800 Rorug04G0452700 Rorug06G0140300 Rorug06G0140500 Rorug06G0140600
rosa_samantha	Rh1CG223600 Rh1CG227200 Rh1CG227500 Rh1CG227600 Rh2BG341200 Rh2BG345300 Rh2BG440200 Rh2DG284500 Rh2DG357900 Rh2DG362100 Rh2DG362600 Rh2DG364500 Rh2DG364600 Rh2DG364700 Rh2DG364800 Rh2DG364900 Rh2DG365000 Rh2DG365400 Rh2DG450400 Rh3AG080000 Rh3BG081900 Rh5AG322100 Rh5BG079800 Rh6AG252100 Rh6BG255600
rosa_wichuraiana	Rw1G020770 Rw1G021290 Rw2G022120 Rw2G026940 Rw2G027360 Rw2G027370 Rw2G027950 Rw2G027960 Rw2G027970 Rw2G035220 Rw3G006890 Rw6G021900 Rw6G021930 Rw6G021950 Rw6G021970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	24
AciI	CCGC	5 cut(s)	381, 411, 693, 735, 810
AclWI	GGATC	1 cut(s)	43
AcsI	RAATTY	1 cut(s)	158
AcuI	CTGAAG	2 cut(s)	96, 948
AfaI	GTAC	3 cut(s)	312, 604, 896
AfiI	CCNNNNNNNGG	3 cut(s)	58, 364, 590
AflIII	ACRYGT	1 cut(s)	605
AgsI	TTSAA	6 cut(s)	127, 136, 235, 256, 292, 759
AjiI	CACGTC	1 cut(s)	661
AloI	GAACNNNNNNTCC	2 cut(s)	518, 550
AluBI	AGCT	7 cut(s)	22, 65, 350, 433, 472, 890, 926
AluI	AGCT	7 cut(s)	22, 65, 350, 433, 472, 890, 926
Alw21I	GWGCWC	1 cut(s)	474
AlwI	GGATC	1 cut(s)	43
AoxI	GGCC	3 cut(s)	259, 373, 681
ApeKI	GCWGC	4 cut(s)	19, 635, 920, 923
ApoI	RAATTY	1 cut(s)	158
ArsI	GACNNNNNNTTYG	2 cut(s)	183, 215
AspS9I	GGNCC	4 cut(s)	69, 259, 367, 682
AsuHPI	GGTGA	1 cut(s)	763
AsuNHI	GCTAGC	1 cut(s)	731
AvaII	GGWCC	2 cut(s)	69, 367
BanII	GRGCYC	1 cut(s)	474
BbsI	GAAGAC	2 cut(s)	504, 760
Bbv12I	GWGCWC	1 cut(s)	474
BbvI	GCAGC	4 cut(s)	31, 647, 907, 935
BccI	CCATC	4 cut(s)	593, 635, 731, 830
BfaI	CTAG	3 cut(s)	732, 794, 854
BfmI	CTRYAG	2 cut(s)	17, 921
BfuAI	ACCTGC	1 cut(s)	24
BisI	GCNGC	5 cut(s)	20, 411, 636, 921, 924
BlsI	GCNGC	5 cut(s)	21, 412, 637, 922, 925
Bme18I	GGWCC	2 cut(s)	69, 367
BmgBI	CACGTC	1 cut(s)	661
BmgT120I	GGNCC	4 cut(s)	69, 259, 367, 682
BmiI	GGNNCC	1 cut(s)	739
BmsI	GCATC	3 cut(s)	163, 520, 718
BmtI	GCTAGC	1 cut(s)	735
BpiI	GAAGAC	2 cut(s)	504, 760
Bsa29I	ATCGAT	1 cut(s)	726
BsaJI	CCNNGG	3 cut(s)	370, 502, 799
BsaWI	WCCGGW	1 cut(s)	773
Bsc4I	CCNNNNNNNGG	3 cut(s)	58, 364, 590
Bse118I	RCCGGY	1 cut(s)	664
Bse1I	ACTGG	4 cut(s)	376, 445, 538, 652
Bse3DI	GCAATG	1 cut(s)	630
BseCI	ATCGAT	1 cut(s)	726
BseDI	CCNNGG	3 cut(s)	370, 502, 799
BseGI	GGATG	3 cut(s)	389, 543, 841
BseLI	CCNNNNNNNGG	3 cut(s)	58, 364, 590
BseMI	GCAATG	1 cut(s)	630
BseMII	CTCAG	1 cut(s)	453
BseNI	ACTGG	4 cut(s)	376, 445, 538, 652
BseXI	GCAGC	4 cut(s)	31, 647, 907, 935
BseYI	CCCAGC	2 cut(s)	491, 890
BsgI	GTGCAG	1 cut(s)	894
BshFI	GGCC	3 cut(s)	261, 375, 683
BshVI	ATCGAT	1 cut(s)	726
BsiHKAI	GWGCWC	1 cut(s)	474
BsiSI	CCGG	2 cut(s)	665, 774
BslFI	GGGAC	1 cut(s)	853
BslI	CCNNNNNNNGG	3 cut(s)	58, 364, 590
BsmFI	GGGAC	1 cut(s)	853
BsmI	GAATGC	1 cut(s)	909
BsnI	GGCC	3 cut(s)	261, 375, 683
Bsp1286I	GDGCHC	1 cut(s)	474
Bsp143I	GATC	3 cut(s)	6, 48, 450
Bsp19I	CCATGG	1 cut(s)	799
BspACI	CCGC	5 cut(s)	381, 411, 693, 735, 810
BspANI	GGCC	3 cut(s)	261, 375, 683
BspCNI	CTCAG	1 cut(s)	454
BspDI	ATCGAT	1 cut(s)	726
BspLI	GGNNCC	1 cut(s)	739
BspMAI	CTGCAG	2 cut(s)	21, 925
BspMI	ACCTGC	1 cut(s)	24
BspOI	GCTAGC	1 cut(s)	735
BspPI	GGATC	1 cut(s)	43
BsrDI	GCAATG	1 cut(s)	630
BsrFI	RCCGGY	1 cut(s)	664
BsrI	ACTGG	4 cut(s)	376, 445, 538, 652
BssAI	RCCGGY	1 cut(s)	664
BssECI	CCNNGG	3 cut(s)	370, 502, 799
BssMI	GATC	3 cut(s)	6, 48, 450
BssT1I	CCWWGG	3 cut(s)	370, 502, 799
Bst4CI	ACNGT	2 cut(s)	310, 593
Bst6I	CTCTTC	1 cut(s)	222
BstC8I	GCNNGC	2 cut(s)	666, 733
BstDEI	CTNAG	2 cut(s)	462, 843
BstDSI	CCRYGG	1 cut(s)	799
BstF5I	GGATG	3 cut(s)	389, 543, 841
BstKTI	GATC	3 cut(s)	9, 51, 453
BstMBI	GATC	3 cut(s)	6, 48, 450
BstMWI	GCNNNNNNNGC	4 cut(s)	401, 410, 632, 728
BstNSI	RCATGY	1 cut(s)	609
BstSFI	CTRYAG	2 cut(s)	17, 921
BstV1I	GCAGC	4 cut(s)	31, 647, 907, 935
BstV2I	GAAGAC	2 cut(s)	504, 760
BstX2I	RGATCY	1 cut(s)	6
BstYI	RGATCY	1 cut(s)	6
Bsu15I	ATCGAT	1 cut(s)	726
BsuRI	GGCC	3 cut(s)	261, 375, 683
BsuTUI	ATCGAT	1 cut(s)	726
BtgI	CCRYGG	1 cut(s)	799
BtrI	CACGTC	1 cut(s)	661
BtsCI	GGATG	3 cut(s)	389, 543, 841
BtsI	GCAGTG	1 cut(s)	486
BtsIMutI	CAGTG	2 cut(s)	383, 486
BveI	ACCTGC	1 cut(s)	24
Cac8I	GCNNGC	2 cut(s)	666, 733
Cfr10I	RCCGGY	1 cut(s)	664
Cfr13I	GGNCC	4 cut(s)	69, 259, 367, 682
ClaI	ATCGAT	1 cut(s)	726
Csp6I	GTAC	3 cut(s)	311, 603, 895
CviAII	CATG	5 cut(s)	55, 263, 606, 800, 867
CviQI	GTAC	3 cut(s)	311, 603, 895
DdeI	CTNAG	2 cut(s)	462, 843
DpnI	GATC	3 cut(s)	8, 50, 452
DpnII	GATC	3 cut(s)	6, 48, 450
Eam1104I	CTCTTC	1 cut(s)	222
EarI	CTCTTC	1 cut(s)	222
Ecl136II	GAGCTC	1 cut(s)	472
Eco130I	CCWWGG	3 cut(s)	370, 502, 799
Eco24I	GRGCYC	1 cut(s)	474
Eco47I	GGWCC	2 cut(s)	69, 367
Eco53kI	GAGCTC	1 cut(s)	472
Eco57I	CTGAAG	2 cut(s)	96, 948
EcoICRI	GAGCTC	1 cut(s)	472
EcoO109I	RGGNCCY	1 cut(s)	682
EcoT14I	CCWWGG	3 cut(s)	370, 502, 799
EcoT38I	GRGCYC	1 cut(s)	474
ErhI	CCWWGG	3 cut(s)	370, 502, 799
FaeI	CATG	5 cut(s)	58, 266, 609, 803, 870
FaqI	GGGAC	1 cut(s)	853
FatI	CATG	5 cut(s)	54, 262, 605, 799, 866
Fnu4HI	GCNGC	5 cut(s)	20, 411, 636, 921, 924
FokI	GGATG	3 cut(s)	396, 530, 848
FriOI	GRGCYC	1 cut(s)	474
Fsp4HI	GCNGC	5 cut(s)	20, 411, 636, 921, 924
FspBI	CTAG	3 cut(s)	732, 794, 854
GluI	GCNGC	5 cut(s)	20, 411, 636, 921, 924
GsaI	CCCAGC	2 cut(s)	495, 894
HaeIII	GGCC	3 cut(s)	261, 375, 683
HapII	CCGG	2 cut(s)	665, 774
Hin1II	CATG	5 cut(s)	58, 266, 609, 803, 870
HincII	GTYRAC	1 cut(s)	202
HindII	GTYRAC	1 cut(s)	202
HinfI	GANTC	2 cut(s)	252, 790
HpaII	CCGG	2 cut(s)	665, 774
HphI	GGTGA	1 cut(s)	763
Hpy166II	GTNNAC	1 cut(s)	202
Hpy188I	TCNGA	3 cut(s)	76, 463, 742
Hpy188III	TCNNGA	2 cut(s)	127, 794
Hpy8I	GTNNAC	1 cut(s)	202
HpyAV	CCTTC	5 cut(s)	106, 413, 613, 695, 923
HpyCH4III	ACNGT	2 cut(s)	310, 593
HpyCH4IV	ACGT	1 cut(s)	660
HpyCH4V	TGCA	6 cut(s)	19, 176, 635, 875, 907, 923
HpyF10VI	GCNNNNNNNGC	4 cut(s)	401, 410, 632, 728
HpyF3I	CTNAG	2 cut(s)	462, 843
HpySE526I	ACGT	1 cut(s)	660
Hsp92II	CATG	5 cut(s)	58, 266, 609, 803, 870
KroI	GCCGGC	1 cut(s)	664
KroNI	GCCGGC	1 cut(s)	666
Kzo9I	GATC	3 cut(s)	6, 48, 450
LmnI	GCTCC	2 cut(s)	62, 347
Lsp1109I	GCAGC	4 cut(s)	31, 647, 907, 935
LweI	GCATC	3 cut(s)	163, 520, 718
MaeI	CTAG	3 cut(s)	732, 794, 854
MaeII	ACGT	1 cut(s)	660
MaeIII	GTNAC	6 cut(s)	304, 352, 443, 482, 608, 805
MalI	GATC	3 cut(s)	8, 50, 452
MboI	GATC	3 cut(s)	6, 48, 450
MboII	GAAGA	5 cut(s)	239, 276, 509, 760, 871
MflI	RGATCY	1 cut(s)	6
MhlI	GDGCHC	1 cut(s)	474
MluCI	AATT	6 cut(s)	158, 191, 206, 423, 577, 749
MlyI	GAGTC	1 cut(s)	799
MmeI	TCCRAC	3 cut(s)	571, 765, 796
MnlI	CCTC	5 cut(s)	62, 82, 505, 649, 826
MroNI	GCCGGC	1 cut(s)	664
MseI	TTAA	2 cut(s)	194, 363
MslI	CAYNNNNRTG	1 cut(s)	902
MspA1I	CMGCKG	3 cut(s)	695, 812, 926
MspI	CCGG	2 cut(s)	665, 774
Mva1269I	GAATGC	1 cut(s)	909
MwoI	GCNNNNNNNGC	4 cut(s)	401, 410, 632, 728
NaeI	GCCGGC	1 cut(s)	666
NcoI	CCATGG	1 cut(s)	799
NdeII	GATC	3 cut(s)	6, 48, 450
NgoMIV	GCCGGC	1 cut(s)	664
NheI	GCTAGC	1 cut(s)	731
NlaIII	CATG	5 cut(s)	58, 266, 609, 803, 870
NlaIV	GGNNCC	1 cut(s)	739
NmuCI	GTSAC	2 cut(s)	304, 482
NspI	RCATGY	1 cut(s)	609
PciI	ACATGT	1 cut(s)	605
PctI	GAATGC	1 cut(s)	909
PdiI	GCCGGC	1 cut(s)	666
PfeI	GAWTC	1 cut(s)	252
PkrI	GCNGC	5 cut(s)	21, 412, 637, 922, 925
PleI	GAGTC	1 cut(s)	798
PpsI	GAGTC	1 cut(s)	798
PscI	ACATGT	1 cut(s)	605
Psp124BI	GAGCTC	1 cut(s)	474
PspFI	CCCAGC	2 cut(s)	491, 890
PspN4I	GGNNCC	1 cut(s)	739
PspPI	GGNCC	4 cut(s)	69, 259, 367, 682
PstI	CTGCAG	2 cut(s)	21, 925
PsuI	RGATCY	1 cut(s)	6
PvuII	CAGCTG	1 cut(s)	926
RsaI	GTAC	3 cut(s)	312, 604, 896
RsaNI	GTAC	3 cut(s)	311, 603, 895
RseI	CAYNNNNRTG	1 cut(s)	902
SacI	GAGCTC	1 cut(s)	474
SaqAI	TTAA	2 cut(s)	194, 363
SatI	GCNGC	5 cut(s)	20, 411, 636, 921, 924
Sau3AI	GATC	3 cut(s)	6, 48, 450
Sau96I	GGNCC	4 cut(s)	69, 259, 367, 682
SchI	GAGTC	1 cut(s)	799
SduI	GDGCHC	1 cut(s)	474
SfaNI	GCATC	3 cut(s)	163, 520, 718
SfcI	CTRYAG	2 cut(s)	17, 921
SinI	GGWCC	2 cut(s)	69, 367
SmiMI	CAYNNNNRTG	1 cut(s)	902
Sse9I	AATT	6 cut(s)	158, 191, 206, 423, 577, 749
SsiI	CCGC	5 cut(s)	381, 411, 693, 735, 810
SspMI	CTAG	3 cut(s)	732, 794, 854
SstI	GAGCTC	1 cut(s)	474
StyI	CCWWGG	3 cut(s)	370, 502, 799
TaaI	ACNGT	2 cut(s)	310, 593
TaiI	ACGT	1 cut(s)	663
TaqI	TCGA	1 cut(s)	726
TasI	AATT	6 cut(s)	158, 191, 206, 423, 577, 749
TauI	GCSGC	1 cut(s)	413
TfiI	GAWTC	1 cut(s)	252
Tru1I	TTAA	2 cut(s)	194, 363
Tru9I	TTAA	2 cut(s)	194, 363
TscAI	CASTG	2 cut(s)	383, 493
TseFI	GTSAC	2 cut(s)	304, 482
TseI	GCWGC	4 cut(s)	19, 635, 920, 923
Tsp45I	GTSAC	2 cut(s)	304, 482
TspDTI	ATGAA	3 cut(s)	240, 312, 532
TspGWI	ACGGA	1 cut(s)	706
TspRI	CASTG	2 cut(s)	383, 493
VpaK11BI	GGWCC	2 cut(s)	69, 367
XapI	RAATTY	1 cut(s)	158
XbaI	TCTAGA	1 cut(s)	793
XceI	RCATGY	1 cut(s)	609
XspI	CTAG	3 cut(s)	732, 794, 854

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.