Leucine Rich repeat
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 441 |
| AarI | CACCTGC | 1 cut(s) | 503 |
| Acc36I | ACCTGC | 2 cut(s) | 42, 503 |
| AccB1I | GGYRCC | 1 cut(s) | 2456 |
| AccBSI | CCGCTC | 1 cut(s) | 1397 |
| AccII | CGCG | 1 cut(s) | 2570 |
| AccIII | TCCGGA | 1 cut(s) | 1190 |
| AciI | CCGC | 4 cut(s) | 1395, 2519, 2676, 2773 |
| AclI | AACGTT | 1 cut(s) | 1087 |
| AclWI | GGATC | 2 cut(s) | 1435, 1458 |
| AcoI | YGGCCR | 4 cut(s) | 132, 689, 1073, 1939 |
| AcsI | RAATTY | 6 cut(s) | 1195, 1753, 1853, 2229, 2373, 2445 |
| AcuI | CTGAAG | 3 cut(s) | 713, 2328, 2450 |
| AdeI | CACNNNGTG | 1 cut(s) | 2240 |
| AfaI | GTAC | 2 cut(s) | 584, 1876 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 93, 380, 548, 1063, 1447, 2467, 2543 |
| AflII | CTTAAG | 1 cut(s) | 2690 |
| AflIII | ACRYGT | 1 cut(s) | 654 |
| AjnI | CCWGG | 5 cut(s) | 129, 260, 1070, 1440, 2536 |
| Alw21I | GWGCWC | 2 cut(s) | 996, 1295 |
| Alw26I | GTCTC | 3 cut(s) | 1028, 1257, 2618 |
| AlwI | GGATC | 2 cut(s) | 1435, 1458 |
| Ama87I | CYCGRG | 1 cut(s) | 1145 |
| Aor13HI | TCCGGA | 1 cut(s) | 1190 |
| AoxI | GGCC | 6 cut(s) | 132, 229, 689, 1073, 1351, 1939 |
| ApaI | GGGCCC | 1 cut(s) | 1355 |
| ApeKI | GCWGC | 4 cut(s) | 282, 867, 942, 2024 |
| ApoI | RAATTY | 6 cut(s) | 1195, 1753, 1853, 2229, 2373, 2445 |
| AseI | ATTAAT | 1 cut(s) | 783 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2178 |
| AspLEI | GCGC | 1 cut(s) | 1571 |
| AspS9I | GGNCC | 8 cut(s) | 430, 530, 1068, 1351, 1352, 1631, 2041, 2513 |
| AsuHPI | GGTGA | 5 cut(s) | 76, 319, 395, 1972, 2174 |
| AvaI | CYCGRG | 1 cut(s) | 1145 |
| AvaII | GGWCC | 6 cut(s) | 430, 530, 1068, 1631, 2041, 2513 |
| BaeGI | GKGCMC | 1 cut(s) | 1355 |
| BalI | TGGCCA | 4 cut(s) | 134, 691, 1075, 1941 |
| BanI | GGYRCC | 1 cut(s) | 2456 |
| BanII | GRGCYC | 2 cut(s) | 1295, 1355 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 900, 932 |
| BbsI | GAAGAC | 3 cut(s) | 1542, 1632, 2316 |
| Bbv12I | GWGCWC | 2 cut(s) | 996, 1295 |
| BbvI | GCAGC | 4 cut(s) | 269, 879, 954, 2011 |
| BccI | CCATC | 4 cut(s) | 1585, 2555, 2603, 2662 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1513, 1547 |
| BciT130I | CCWGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| BciVI | GTATCC | 1 cut(s) | 909 |
| BclI | TGATCA | 1 cut(s) | 459 |
| BcoDI | GTCTC | 3 cut(s) | 1028, 1257, 2618 |
| BfaI | CTAG | 5 cut(s) | 665, 1167, 1256, 2021, 2280 |
| BfmI | CTRYAG | 1 cut(s) | 1805 |
| BfrI | CTTAAG | 1 cut(s) | 2690 |
| BfuAI | ACCTGC | 2 cut(s) | 42, 503 |
| BfuI | GTATCC | 1 cut(s) | 909 |
| BglII | AGATCT | 1 cut(s) | 1477 |
| BisI | GCNGC | 5 cut(s) | 283, 868, 943, 2025, 2677 |
| BlsI | GCNGC | 5 cut(s) | 284, 869, 944, 2026, 2678 |
| Bme1390I | CCNGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| Bme18I | GGWCC | 6 cut(s) | 430, 530, 1068, 1631, 2041, 2513 |
| BmeT110I | CYCGRG | 1 cut(s) | 1145 |
| BmgT120I | GGNCC | 8 cut(s) | 430, 530, 1068, 1351, 1352, 1631, 2041, 2513 |
| BmiI | GGNNCC | 4 cut(s) | 1069, 1353, 1662, 2458 |
| BmrFI | CCNGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| BmrI | ACTGGG | 1 cut(s) | 733 |
| BmsI | GCATC | 1 cut(s) | 1716 |
| BmuI | ACTGGG | 1 cut(s) | 733 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1636 |
| BpiI | GAAGAC | 3 cut(s) | 1542, 1632, 2316 |
| BpmI | CTGGAG | 2 cut(s) | 200, 1647 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1289 |
| BpuEI | CTTGAG | 4 cut(s) | 635, 661, 1052, 1349 |
| BsaBI | GATNNNNATC | 2 cut(s) | 759, 1302 |
| BsaI | GGTCTC | 2 cut(s) | 1028, 2618 |
| BsaJI | CCNNGG | 6 cut(s) | 261, 1038, 1498, 1897, 2620, 2760 |
| BsaWI | WCCGGW | 3 cut(s) | 1190, 1877, 2358 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 1645, 1675, 2767 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 93, 380, 548, 1063, 1447, 2467, 2543 |
| Bse1I | ACTGG | 3 cut(s) | 217, 739, 2662 |
| Bse3DI | GCAATG | 4 cut(s) | 135, 1430, 1571, 2077 |
| Bse8I | GATNNNNATC | 2 cut(s) | 759, 1302 |
| BseAI | TCCGGA | 1 cut(s) | 1190 |
| BseBI | CCWGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| BseDI | CCNNGG | 6 cut(s) | 261, 1038, 1498, 1897, 2620, 2760 |
| BseGI | GGATG | 3 cut(s) | 244, 1731, 2673 |
| BseJI | GATNNNNATC | 2 cut(s) | 759, 1302 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 93, 380, 548, 1063, 1447, 2467, 2543 |
| BseMI | GCAATG | 4 cut(s) | 135, 1430, 1571, 2077 |
| BseMII | CTCAG | 3 cut(s) | 448, 1011, 1280 |
| BseNI | ACTGG | 3 cut(s) | 217, 739, 2662 |
| BseSI | GKGCMC | 1 cut(s) | 1355 |
| BseXI | GCAGC | 4 cut(s) | 269, 879, 954, 2011 |
| BseYI | CCCAGC | 1 cut(s) | 2644 |
| BsgI | GTGCAG | 2 cut(s) | 2035, 2658 |
| Bsh1236I | CGCG | 1 cut(s) | 2570 |
| BshFI | GGCC | 6 cut(s) | 134, 231, 691, 1075, 1353, 1941 |
| BshNI | GGYRCC | 1 cut(s) | 2456 |
| BsiHKAI | GWGCWC | 2 cut(s) | 996, 1295 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1145 |
| BsiSI | CCGG | 3 cut(s) | 1191, 1878, 2359 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 93, 380, 548, 1063, 1447, 2467, 2543 |
| BsmAI | GTCTC | 3 cut(s) | 1028, 1257, 2618 |
| BsnI | GGCC | 6 cut(s) | 134, 231, 691, 1075, 1353, 1941 |
| Bso31I | GGTCTC | 2 cut(s) | 1028, 2618 |
| BsoBI | CYCGRG | 1 cut(s) | 1145 |
| Bsp120I | GGGCCC | 1 cut(s) | 1351 |
| Bsp1286I | GDGCHC | 3 cut(s) | 996, 1295, 1355 |
| Bsp13I | TCCGGA | 1 cut(s) | 1190 |
| Bsp143I | GATC | 8 cut(s) | 459, 577, 767, 886, 957, 1427, 1450, 1477 |
| Bsp19I | CCATGG | 1 cut(s) | 1498 |
| Bsp68I | TCGCGA | 1 cut(s) | 2570 |
| BspACI | CCGC | 4 cut(s) | 1395, 2519, 2676, 2773 |
| BspANI | GGCC | 6 cut(s) | 134, 231, 691, 1075, 1353, 1941 |
| BspCNI | CTCAG | 3 cut(s) | 447, 1012, 1281 |
| BspEI | TCCGGA | 1 cut(s) | 1190 |
| BspFNI | CGCG | 1 cut(s) | 2570 |
| BspHI | TCATGA | 1 cut(s) | 751 |
| BspLI | GGNNCC | 4 cut(s) | 1069, 1353, 1662, 2458 |
| BspMI | ACCTGC | 2 cut(s) | 42, 503 |
| BspPI | GGATC | 2 cut(s) | 1435, 1458 |
| BspQI | GCTCTTC | 1 cut(s) | 216 |
| BspT107I | GGYRCC | 1 cut(s) | 2456 |
| BspTI | CTTAAG | 1 cut(s) | 2690 |
| BspTNI | GGTCTC | 2 cut(s) | 1028, 2618 |
| BsrBI | CCGCTC | 1 cut(s) | 1397 |
| BsrDI | GCAATG | 4 cut(s) | 135, 1430, 1571, 2077 |
| BsrI | ACTGG | 3 cut(s) | 217, 739, 2662 |
| BssECI | CCNNGG | 6 cut(s) | 261, 1038, 1498, 1897, 2620, 2760 |
| BssMI | GATC | 8 cut(s) | 459, 577, 767, 886, 957, 1427, 1450, 1477 |
| BssT1I | CCWWGG | 5 cut(s) | 1038, 1498, 1897, 2620, 2760 |
| Bst2UI | CCWGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| Bst4CI | ACNGT | 6 cut(s) | 587, 1251, 1874, 1977, 2186, 2551 |
| Bst6I | CTCTTC | 3 cut(s) | 188, 216, 804 |
| BstAFI | CTTAAG | 1 cut(s) | 2690 |
| BstC8I | GCNNGC | 6 cut(s) | 207, 707, 1532, 1659, 2072, 2392 |
| BstDSI | CCRYGG | 1 cut(s) | 1498 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 378 |
| BstF5I | GGATG | 3 cut(s) | 244, 1731, 2673 |
| BstFNI | CGCG | 1 cut(s) | 2570 |
| BstHHI | GCGC | 1 cut(s) | 1571 |
| BstKTI | GATC | 8 cut(s) | 462, 580, 770, 889, 960, 1430, 1453, 1480 |
| BstMAI | GTCTC | 3 cut(s) | 1028, 1257, 2618 |
| BstMBI | GATC | 8 cut(s) | 459, 577, 767, 886, 957, 1427, 1450, 1477 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1350, 1658, 1797 |
| BstNI | CCWGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| BstNSI | RCATGY | 2 cut(s) | 209, 658 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1636 |
| BstSCI | CCNGG | 5 cut(s) | 129, 260, 1070, 1440, 2536 |
| BstSFI | CTRYAG | 1 cut(s) | 1805 |
| BstSLI | GKGCMC | 1 cut(s) | 1355 |
| BstUI | CGCG | 1 cut(s) | 2570 |
| BstV1I | GCAGC | 4 cut(s) | 269, 879, 954, 2011 |
| BstV2I | GAAGAC | 3 cut(s) | 1542, 1632, 2316 |
| BstX2I | RGATCY | 2 cut(s) | 1450, 1477 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1499, 1671 |
| BstYI | RGATCY | 2 cut(s) | 1450, 1477 |
| BsuI | GTATCC | 1 cut(s) | 909 |
| BsuRI | GGCC | 6 cut(s) | 134, 231, 691, 1075, 1353, 1941 |
| BtgI | CCRYGG | 1 cut(s) | 1498 |
| BtsCI | GGATG | 3 cut(s) | 244, 1731, 2673 |
| BtsI | GCAGTG | 1 cut(s) | 2646 |
| BtsIMutI | CAGTG | 4 cut(s) | 532, 746, 2646, 2655 |
| BtuMI | TCGCGA | 1 cut(s) | 2570 |
| BveI | ACCTGC | 2 cut(s) | 42, 503 |
| Cac8I | GCNNGC | 6 cut(s) | 207, 707, 1532, 1659, 2072, 2392 |
| CciI | TCATGA | 1 cut(s) | 751 |
| CfoI | GCGC | 1 cut(s) | 1571 |
| Cfr13I | GGNCC | 8 cut(s) | 430, 530, 1068, 1351, 1352, 1631, 2041, 2513 |
| Csp6I | GTAC | 2 cut(s) | 583, 1875 |
| CviQI | GTAC | 2 cut(s) | 583, 1875 |
| DpnI | GATC | 8 cut(s) | 461, 579, 769, 888, 959, 1429, 1452, 1479 |
| DpnII | GATC | 8 cut(s) | 459, 577, 767, 886, 957, 1427, 1450, 1477 |
| DraIII | CACNNNGTG | 1 cut(s) | 2240 |
| EaeI | YGGCCR | 4 cut(s) | 132, 689, 1073, 1939 |
| Eam1104I | CTCTTC | 3 cut(s) | 188, 216, 804 |
| EarI | CTCTTC | 3 cut(s) | 188, 216, 804 |
| Ecl136II | GAGCTC | 1 cut(s) | 1293 |
| Eco130I | CCWWGG | 5 cut(s) | 1038, 1498, 1897, 2620, 2760 |
| Eco147I | AGGCCT | 1 cut(s) | 231 |
| Eco24I | GRGCYC | 2 cut(s) | 1295, 1355 |
| Eco31I | GGTCTC | 2 cut(s) | 1028, 2618 |
| Eco32I | GATATC | 2 cut(s) | 416, 2364 |
| Eco47I | GGWCC | 6 cut(s) | 430, 530, 1068, 1631, 2041, 2513 |
| Eco53kI | GAGCTC | 1 cut(s) | 1293 |
| Eco57I | CTGAAG | 3 cut(s) | 713, 2328, 2450 |
| Eco88I | CYCGRG | 1 cut(s) | 1145 |
| EcoICRI | GAGCTC | 1 cut(s) | 1293 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 378 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1068, 1631 |
| EcoRI | GAATTC | 1 cut(s) | 2445 |
| EcoRII | CCWGG | 5 cut(s) | 129, 260, 1070, 1440, 2536 |
| EcoRV | GATATC | 2 cut(s) | 416, 2364 |
| EcoT14I | CCWWGG | 5 cut(s) | 1038, 1498, 1897, 2620, 2760 |
| EcoT38I | GRGCYC | 2 cut(s) | 1295, 1355 |
| ErhI | CCWWGG | 5 cut(s) | 1038, 1498, 1897, 2620, 2760 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2695, 2727 |
| FbaI | TGATCA | 1 cut(s) | 459 |
| Fnu4HI | GCNGC | 5 cut(s) | 283, 868, 943, 2025, 2677 |
| FokI | GGATG | 3 cut(s) | 251, 1738, 2680 |
| FriOI | GRGCYC | 2 cut(s) | 1295, 1355 |
| Fsp4HI | GCNGC | 5 cut(s) | 283, 868, 943, 2025, 2677 |
| FspBI | CTAG | 5 cut(s) | 665, 1167, 1256, 2021, 2280 |
| GlaI | GCGC | 1 cut(s) | 1570 |
| GluI | GCNGC | 5 cut(s) | 283, 868, 943, 2025, 2677 |
| GsaI | CCCAGC | 1 cut(s) | 2648 |
| GsuI | CTGGAG | 2 cut(s) | 200, 1647 |
| HaeIII | GGCC | 6 cut(s) | 134, 231, 691, 1075, 1353, 1941 |
| HapII | CCGG | 3 cut(s) | 1191, 1878, 2359 |
| HhaI | GCGC | 1 cut(s) | 1571 |
| Hin6I | GCGC | 1 cut(s) | 1569 |
| HinP1I | GCGC | 1 cut(s) | 1569 |
| HincII | GTYRAC | 1 cut(s) | 1894 |
| HindII | GTYRAC | 1 cut(s) | 1894 |
| HindIII | AAGCTT | 2 cut(s) | 1528, 2300 |
| HpaII | CCGG | 3 cut(s) | 1191, 1878, 2359 |
| HphI | GGTGA | 5 cut(s) | 76, 319, 395, 1972, 2174 |
| Hpy166II | GTNNAC | 6 cut(s) | 530, 1247, 1829, 1894, 2513, 2684 |
| Hpy188I | TCNGA | 4 cut(s) | 607, 982, 1297, 2271 |
| Hpy8I | GTNNAC | 6 cut(s) | 530, 1247, 1829, 1894, 2513, 2684 |
| HpyAV | CCTTC | 6 cut(s) | 758, 902, 1869, 2014, 2143, 2537 |
| HpyCH4III | ACNGT | 6 cut(s) | 587, 1251, 1874, 1977, 2186, 2551 |
| HpyCH4IV | ACGT | 2 cut(s) | 1087, 1325 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1350, 1658, 1797 |
| HpySE526I | ACGT | 2 cut(s) | 1087, 1325 |
| HspAI | GCGC | 1 cut(s) | 1569 |
| Kpn2I | TCCGGA | 1 cut(s) | 1190 |
| Ksp22I | TGATCA | 1 cut(s) | 459 |
| Kzo9I | GATC | 8 cut(s) | 459, 577, 767, 886, 957, 1427, 1450, 1477 |
| LguI | GCTCTTC | 1 cut(s) | 216 |
| LmnI | GCTCC | 3 cut(s) | 176, 1666, 1708 |
| Lsp1109I | GCAGC | 4 cut(s) | 269, 879, 954, 2011 |
| LweI | GCATC | 1 cut(s) | 1716 |
| MaeI | CTAG | 5 cut(s) | 665, 1167, 1256, 2021, 2280 |
| MaeII | ACGT | 2 cut(s) | 1087, 1325 |
| MaeIII | GTNAC | 6 cut(s) | 305, 325, 587, 743, 2318, 2653 |
| MalI | GATC | 8 cut(s) | 461, 579, 769, 888, 959, 1429, 1452, 1479 |
| MbiI | CCGCTC | 1 cut(s) | 1397 |
| MboI | GATC | 8 cut(s) | 459, 577, 767, 886, 957, 1427, 1450, 1477 |
| MfeI | CAATTG | 1 cut(s) | 491 |
| MflI | RGATCY | 2 cut(s) | 1450, 1477 |
| MhlI | GDGCHC | 3 cut(s) | 996, 1295, 1355 |
| MlsI | TGGCCA | 4 cut(s) | 134, 691, 1075, 1941 |
| MluNI | TGGCCA | 4 cut(s) | 134, 691, 1075, 1941 |
| MlyI | GAGTC | 3 cut(s) | 251, 496, 959 |
| MmeI | TCCRAC | 4 cut(s) | 145, 198, 1703, 2313 |
| Mox20I | TGGCCA | 4 cut(s) | 134, 691, 1075, 1941 |
| MroI | TCCGGA | 1 cut(s) | 1190 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2178 |
| MscI | TGGCCA | 4 cut(s) | 134, 691, 1075, 1941 |
| MslI | CAYNNNNRTG | 2 cut(s) | 74, 2327 |
| Msp20I | TGGCCA | 4 cut(s) | 134, 691, 1075, 1941 |
| MspA1I | CMGCKG | 1 cut(s) | 2521 |
| MspCI | CTTAAG | 1 cut(s) | 2690 |
| MspI | CCGG | 3 cut(s) | 1191, 1878, 2359 |
| MspR9I | CCNGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| MunI | CAATTG | 1 cut(s) | 491 |
| MvaI | CCWGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| MvnI | CGCG | 1 cut(s) | 2570 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1350, 1658, 1797 |
| NcoI | CCATGG | 1 cut(s) | 1498 |
| NdeII | GATC | 8 cut(s) | 459, 577, 767, 886, 957, 1427, 1450, 1477 |
| NlaIV | GGNNCC | 4 cut(s) | 1069, 1353, 1662, 2458 |
| NmuCI | GTSAC | 2 cut(s) | 325, 2653 |
| NruI | TCGCGA | 1 cut(s) | 2570 |
| NspI | RCATGY | 2 cut(s) | 209, 658 |
| PaeI | GCATGC | 1 cut(s) | 209 |
| PagI | TCATGA | 1 cut(s) | 751 |
| PaqCI | CACCTGC | 1 cut(s) | 503 |
| PceI | AGGCCT | 1 cut(s) | 231 |
| PciI | ACATGT | 1 cut(s) | 654 |
| PciSI | GCTCTTC | 1 cut(s) | 216 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2178 |
| PfeI | GAWTC | 8 cut(s) | 1303, 1372, 1513, 1963, 1995, 2336, 2565, 2625 |
| PfoI | TCCNGGA | 1 cut(s) | 2536 |
| PkrI | GCNGC | 5 cut(s) | 284, 869, 944, 2026, 2678 |
| PleI | GAGTC | 3 cut(s) | 250, 495, 958 |
| PpsI | GAGTC | 3 cut(s) | 250, 495, 958 |
| PpuMI | RGGWCCY | 2 cut(s) | 1068, 1631 |
| PscI | ACATGT | 1 cut(s) | 654 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1636 |
| PshBI | ATTAAT | 1 cut(s) | 783 |
| PsiI | TTATAA | 1 cut(s) | 441 |
| Psp124BI | GAGCTC | 1 cut(s) | 1295 |
| Psp1406I | AACGTT | 1 cut(s) | 1087 |
| Psp5II | RGGWCCY | 2 cut(s) | 1068, 1631 |
| Psp6I | CCWGG | 5 cut(s) | 129, 260, 1070, 1440, 2536 |
| PspFI | CCCAGC | 1 cut(s) | 2644 |
| PspGI | CCWGG | 5 cut(s) | 129, 260, 1070, 1440, 2536 |
| PspN4I | GGNNCC | 4 cut(s) | 1069, 1353, 1662, 2458 |
| PspOMI | GGGCCC | 1 cut(s) | 1351 |
| PspPI | GGNCC | 8 cut(s) | 430, 530, 1068, 1351, 1352, 1631, 2041, 2513 |
| PspPPI | RGGWCCY | 2 cut(s) | 1068, 1631 |
| PsuI | RGATCY | 2 cut(s) | 1450, 1477 |
| RruI | TCGCGA | 1 cut(s) | 2570 |
| RsaI | GTAC | 2 cut(s) | 584, 1876 |
| RsaNI | GTAC | 2 cut(s) | 583, 1875 |
| RseI | CAYNNNNRTG | 2 cut(s) | 74, 2327 |
| SacI | GAGCTC | 1 cut(s) | 1295 |
| SapI | GCTCTTC | 1 cut(s) | 216 |
| SatI | GCNGC | 5 cut(s) | 283, 868, 943, 2025, 2677 |
| Sau3AI | GATC | 8 cut(s) | 459, 577, 767, 886, 957, 1427, 1450, 1477 |
| Sau96I | GGNCC | 8 cut(s) | 430, 530, 1068, 1351, 1352, 1631, 2041, 2513 |
| SchI | GAGTC | 3 cut(s) | 251, 496, 959 |
| ScrFI | CCNGG | 5 cut(s) | 131, 262, 1072, 1442, 2538 |
| SduI | GDGCHC | 3 cut(s) | 996, 1295, 1355 |
| SfaNI | GCATC | 1 cut(s) | 1716 |
| SfcI | CTRYAG | 1 cut(s) | 1805 |
| SinI | GGWCC | 6 cut(s) | 430, 530, 1068, 1631, 2041, 2513 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 74, 2327 |
| SmlI | CTYRAG | 5 cut(s) | 614, 640, 1031, 1364, 2690 |
| SmoI | CTYRAG | 5 cut(s) | 614, 640, 1031, 1364, 2690 |
| SphI | GCATGC | 1 cut(s) | 209 |
| SseBI | AGGCCT | 1 cut(s) | 231 |
| SsiI | CCGC | 4 cut(s) | 1395, 2519, 2676, 2773 |
| SspI | AATATT | 2 cut(s) | 843, 2059 |
| SspMI | CTAG | 5 cut(s) | 665, 1167, 1256, 2021, 2280 |
| SstI | GAGCTC | 1 cut(s) | 1295 |
| StuI | AGGCCT | 1 cut(s) | 231 |
| StyD4I | CCNGG | 5 cut(s) | 129, 260, 1070, 1440, 2536 |
| StyI | CCWWGG | 5 cut(s) | 1038, 1498, 1897, 2620, 2760 |
| TaaI | ACNGT | 6 cut(s) | 587, 1251, 1874, 1977, 2186, 2551 |
| TaiI | ACGT | 2 cut(s) | 1090, 1328 |
| TaqI | TCGA | 3 cut(s) | 1523, 1613, 2701 |
| TauI | GCSGC | 1 cut(s) | 2679 |
| TfiI | GAWTC | 8 cut(s) | 1303, 1372, 1513, 1963, 1995, 2336, 2565, 2625 |
| TscAI | CASTG | 4 cut(s) | 532, 746, 2646, 2662 |
| TseFI | GTSAC | 2 cut(s) | 325, 2653 |
| TseI | GCWGC | 4 cut(s) | 282, 867, 942, 2024 |
| Tsp45I | GTSAC | 2 cut(s) | 325, 2653 |
| TspDTI | ATGAA | 7 cut(s) | 58, 463, 740, 1502, 2021, 2339, 2707 |
| TspRI | CASTG | 4 cut(s) | 532, 746, 2646, 2662 |
| Vha464I | CTTAAG | 1 cut(s) | 2690 |
| VpaK11BI | GGWCC | 6 cut(s) | 430, 530, 1068, 1631, 2041, 2513 |
| VspI | ATTAAT | 1 cut(s) | 783 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 378 |
| XapI | RAATTY | 6 cut(s) | 1195, 1753, 1853, 2229, 2373, 2445 |
| XbaI | TCTAGA | 2 cut(s) | 664, 2279 |
| XceI | RCATGY | 2 cut(s) | 209, 658 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2035 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2178 |
| XspI | CTAG | 5 cut(s) | 665, 1167, 1256, 2021, 2280 |