AT2G02250

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000189
Physical Location & Seq
Reverse (-)
594484 .. 595869
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G02250.1

Sequence Viewer

Length: 933 bp
ATGATACAATCAACAATGGGACACAAACAAAGCGTTGACTCTAGAGGCAAAGGCCGAAAAGTTCCCGGTTCTTCGTCGATGGTGCAAAAACATAGAGTTGAATCTAGCGGAGGAGCTATCATCTCCGGACCGTCACTGTTCGATAACTTACCGGAGGATTGCATCTCCAACATCATCTCTTTCACGAGTCCTCGTGACGCTTGCGTTGCAGCTTCGGTTTCGAAAACCTTTGAATCGGCTGTTAATTCCGATAGCGTATGGGACAAGTTTCTTCCATCGGATTATTCATCTCTAGTTCCTCCATCTCGAGTTTTCTCATCGAAGAAGGAGCTCTATTTCGCTATATGCGACAATCCTGTTCTAGTCGAAGATGGCGGAAAGAGCTTCTGGTTAGAGAAAGAGAATGGGAAAAAGTGTTTCATGTTATCTCCGAAGAAATCTATGTGGATCACATGGGTTAGTACTCCTCAGTATTGGCGATGGATTTCGATTCCTGAAGCTAGGTTTGAAGAAGTACCAGAGCTGCTCAATGTGTGTTGGTTTGAGGTCCGAGGGGGAATGAATACTAAAGAACTATCTCCTGGAACCCGTTACTCGGCTTACATTGTGTTCAAGACAAAGAATGGATGTCCTAATTTGGGGGATGTGCCAGTAGAAGCTACTGTTGGATTGGTGGGACAAGAATCTTCTCAAAGACACATATACTTTGTTGGGCCTTCGGATCAAAGGAGAGACAGAGAAACGAGAGATGTAACGAGACCAACTAAAAGAAAAGATGGTTGGATGGAAGCTGAGCTTGGTCAATTCTTCAATGAGTCAGGTTGTGATGTTGTTGACACAAGTATTCTTGAGATTAAGACTCCTTATTGGAAGCGCGGTTTGATCATTCAAGGAATCGAATTCCGTCCCACCAAAAGCCTGTTCTATATATAA

Protein Analysis

310

Amino Acids

34.93

Weight (kDa)

8.38

Isoelectric Point (pI)

51.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 47 - 88 6.3e-09 F-box domain
F-box-like PF12937 47 - 90 3.6e-07 F-box-like
PP2 PF14299 137 - 303 9e-54 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 876
AccIII TCCGGA 1 cut(s) 125
AciI CCGC 3 cut(s) 108, 375, 876
AclWI GGATC 2 cut(s) 455, 729
AcsI RAATTY 1 cut(s) 899
AcuI CTGAAG 1 cut(s) 516
AfaI GTAC 2 cut(s) 463, 516
AfiI CCNNNNNNNGG 2 cut(s) 595, 638
AgsI TTSAA 6 cut(s) 101, 233, 509, 613, 811, 890
AjnI CCWGG 1 cut(s) 580
AjuI GAANNNNNNNTTGG 7 cut(s) 648, 680, 763, 780, 795, 812, 905
AluBI AGCT 9 cut(s) 116, 212, 331, 384, 500, 523, 659, 791, 796
AluI AGCT 9 cut(s) 116, 212, 331, 384, 500, 523, 659, 791, 796
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 2 cut(s) 726, 751
AlwI GGATC 2 cut(s) 455, 729
Ama87I CYCGRG 1 cut(s) 306
Aor13HI TCCGGA 1 cut(s) 125
AoxI GGCC 2 cut(s) 52, 713
ApeKI GCWGC 2 cut(s) 209, 523
ApoI RAATTY 1 cut(s) 899
AspLEI GCGC 1 cut(s) 876
AspS9I GGNCC 3 cut(s) 128, 547, 713
AsuC2I CCSGG 1 cut(s) 66
AsuII TTCGAA 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 2 cut(s) 128, 547
BanII GRGCYC 1 cut(s) 333
BauI CACGAG 2 cut(s) 184, 192
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 2 cut(s) 221, 510
BccI CCATC 7 cut(s) 73, 283, 310, 365, 474, 770, 778
BciT130I CCWGG 1 cut(s) 582
BclI TGATCA 1 cut(s) 882
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 726, 751
BfaI CTAG 5 cut(s) 42, 105, 293, 362, 501
BisI GCNGC 2 cut(s) 210, 524
BlpI GCTNAGC 1 cut(s) 792
BlsI GCNGC 2 cut(s) 211, 525
BmcAI AGTACT 1 cut(s) 463
Bme1390I CCNGG 2 cut(s) 66, 582
Bme18I GGWCC 2 cut(s) 128, 547
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 3 cut(s) 128, 547, 713
BmiI GGNNCC 1 cut(s) 586
BmrFI CCNGG 2 cut(s) 66, 582
BmsI GCATC 1 cut(s) 171
Bpu1102I GCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 869
BpuMI CCSGG 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 751
BsaJI CCNNGG 1 cut(s) 550
BsaWI WCCGGW 2 cut(s) 125, 151
Bsc4I CCNNNNNNNGG 2 cut(s) 595, 638
Bse1I ACTGG 1 cut(s) 650
BseAI TCCGGA 1 cut(s) 125
BseBI CCWGG 1 cut(s) 582
BseDI CCNNGG 1 cut(s) 550
BseGI GGATG 3 cut(s) 632, 649, 789
BseLI CCNNNNNNNGG 2 cut(s) 595, 638
BseMII CTCAG 2 cut(s) 482, 783
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 2 cut(s) 126, 456
BseXI GCAGC 2 cut(s) 221, 510
Bsh1236I CGCG 1 cut(s) 876
BshFI GGCC 2 cut(s) 54, 715
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 3 cut(s) 66, 126, 152
BslFI GGGAC 4 cut(s) 33, 275, 690, 891
BslI CCNNNNNNNGG 2 cut(s) 595, 638
BsmAI GTCTC 2 cut(s) 726, 751
BsmFI GGGAC 4 cut(s) 33, 275, 690, 891
BsnI GGCC 2 cut(s) 54, 715
Bso31I GGTCTC 1 cut(s) 751
BsoBI CYCGRG 1 cut(s) 306
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 333
Bsp13I TCCGGA 1 cut(s) 125
Bsp143I GATC 3 cut(s) 447, 721, 882
Bsp1720I GCTNAGC 1 cut(s) 792
BspACI CCGC 3 cut(s) 108, 375, 876
BspANI GGCC 2 cut(s) 54, 715
BspCNI CTCAG 2 cut(s) 481, 784
BspEI TCCGGA 1 cut(s) 125
BspFNI CGCG 1 cut(s) 876
BspLI GGNNCC 1 cut(s) 586
BspPI GGATC 2 cut(s) 455, 729
BspT104I TTCGAA 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 751
BsrI ACTGG 1 cut(s) 650
BssECI CCNNGG 1 cut(s) 550
BssMI GATC 3 cut(s) 447, 721, 882
BssSI CACGAG 2 cut(s) 184, 192
Bst2BI CACGAG 2 cut(s) 184, 192
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 3 cut(s) 132, 138, 664
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 2 cut(s) 468, 792
BstF5I GGATG 3 cut(s) 632, 649, 789
BstFNI CGCG 1 cut(s) 876
BstHHI GCGC 1 cut(s) 876
BstKTI GATC 3 cut(s) 450, 724, 885
BstMAI GTCTC 2 cut(s) 726, 751
BstMBI GATC 3 cut(s) 447, 721, 882
BstMWI GCNNNNNNNGC 2 cut(s) 206, 381
BstNI CCWGG 1 cut(s) 582
BstSCI CCNGG 2 cut(s) 64, 580
BstUI CGCG 1 cut(s) 876
BstV1I GCAGC 2 cut(s) 221, 510
BsuRI GGCC 2 cut(s) 54, 715
BtgZI GCGATG 1 cut(s) 493
BtsCI GGATG 3 cut(s) 632, 649, 789
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 1 cut(s) 202
CfoI GCGC 1 cut(s) 876
Cfr13I GGNCC 3 cut(s) 128, 547, 713
CpoI CGGWCCG 1 cut(s) 128
CseI GACGC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 462, 515
CspI CGGWCCG 1 cut(s) 128
CviAII CATG 2 cut(s) 421, 453
CviQI GTAC 2 cut(s) 462, 515
DdeI CTNAG 2 cut(s) 468, 792
DpnI GATC 3 cut(s) 449, 723, 884
DpnII GATC 3 cut(s) 447, 721, 882
EciI GGCGGA 1 cut(s) 390
Ecl136II GAGCTC 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 751
Eco47I GGWCC 2 cut(s) 128, 547
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 580
EcoT38I GRGCYC 1 cut(s) 333
FaeI CATG 2 cut(s) 424, 456
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 4 cut(s) 33, 275, 690, 891
FatI CATG 2 cut(s) 420, 452
FbaI TGATCA 1 cut(s) 882
Fnu4HI GCNGC 2 cut(s) 210, 524
FokI GGATG 3 cut(s) 639, 656, 796
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 2 cut(s) 210, 524
FspBI CTAG 5 cut(s) 42, 105, 293, 362, 501
GlaI GCGC 1 cut(s) 875
GluI GCNGC 2 cut(s) 210, 524
HaeIII GGCC 2 cut(s) 54, 715
HapII CCGG 3 cut(s) 66, 126, 152
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 876
Hin1II CATG 2 cut(s) 424, 456
Hin6I GCGC 1 cut(s) 874
HinP1I GCGC 1 cut(s) 874
HincII GTYRAC 2 cut(s) 37, 835
HindII GTYRAC 2 cut(s) 37, 835
HinfI GANTC 9 cut(s) 38, 101, 187, 233, 490, 683, 815, 859, 894
HpaII CCGG 3 cut(s) 66, 126, 152
Hpy166II GTNNAC 2 cut(s) 37, 835
Hpy188I TCNGA 5 cut(s) 250, 280, 432, 551, 721
Hpy188III TCNNGA 8 cut(s) 42, 126, 184, 194, 306, 494, 613, 848
Hpy8I GTNNAC 2 cut(s) 37, 835
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 319, 726
HpyCH4III ACNGT 3 cut(s) 132, 138, 664
HpyCH4V TGCA 3 cut(s) 85, 162, 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 381
HpyF3I CTNAG 2 cut(s) 468, 792
Hsp92II CATG 2 cut(s) 424, 456
HspAI GCGC 1 cut(s) 874
Kpn2I TCCGGA 1 cut(s) 125
Ksp22I TGATCA 1 cut(s) 882
Kzo9I GATC 3 cut(s) 447, 721, 882
LmnI GCTCC 2 cut(s) 113, 328
Lsp1109I GCAGC 2 cut(s) 221, 510
LweI GCATC 1 cut(s) 171
MaeI CTAG 5 cut(s) 42, 105, 293, 362, 501
MaeIII GTNAC 4 cut(s) 132, 194, 590, 751
MalI GATC 3 cut(s) 449, 723, 884
MboI GATC 3 cut(s) 447, 721, 882
MboII GAAGA 8 cut(s) 63, 263, 334, 380, 445, 521, 678, 799
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 4 cut(s) 244, 634, 803, 899
MlyI GAGTC 4 cut(s) 32, 196, 824, 853
MmeI TCCRAC 3 cut(s) 192, 646, 761
MnlI CCTC 8 cut(s) 38, 104, 148, 201, 309, 477, 538, 545
MroI TCCGGA 1 cut(s) 125
MseI TTAA 2 cut(s) 243, 855
MspI CCGG 3 cut(s) 66, 126, 152
MspR9I CCNGG 2 cut(s) 66, 582
MvaI CCWGG 1 cut(s) 582
MvnI CGCG 1 cut(s) 876
MwoI GCNNNNNNNGC 2 cut(s) 206, 381
NciI CCSGG 1 cut(s) 66
NdeII GATC 3 cut(s) 447, 721, 882
NlaIII CATG 2 cut(s) 424, 456
NlaIV GGNNCC 1 cut(s) 586
NmeAIII GCCGAG 1 cut(s) 575
NmuCI GTSAC 2 cut(s) 132, 194
NspV TTCGAA 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PfeI GAWTC 5 cut(s) 101, 233, 490, 683, 894
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 2 cut(s) 211, 525
PleI GAGTC 4 cut(s) 32, 195, 823, 853
PpsI GAGTC 4 cut(s) 32, 195, 823, 853
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 580
PspGI CCWGG 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 586
PspPI GGNCC 3 cut(s) 128, 547, 713
RsaI GTAC 2 cut(s) 463, 516
RsaNI GTAC 2 cut(s) 462, 515
Rsr2I CGGWCCG 1 cut(s) 128
RsrII CGGWCCG 1 cut(s) 128
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 243, 855
SatI GCNGC 2 cut(s) 210, 524
Sau3AI GATC 3 cut(s) 447, 721, 882
Sau96I GGNCC 3 cut(s) 128, 547, 713
ScaI AGTACT 1 cut(s) 463
SchI GAGTC 4 cut(s) 32, 196, 824, 853
ScrFI CCNGG 2 cut(s) 66, 582
SduI GDGCHC 1 cut(s) 333
SfaNI GCATC 1 cut(s) 171
Sfr274I CTCGAG 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 2 cut(s) 128, 547
SlaI CTCGAG 1 cut(s) 306
SmlI CTYRAG 2 cut(s) 306, 848
SmoI CTYRAG 2 cut(s) 306, 848
Sse9I AATT 4 cut(s) 244, 634, 803, 899
SsiI CCGC 3 cut(s) 108, 375, 876
SspMI CTAG 5 cut(s) 42, 105, 293, 362, 501
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 64, 580
TaaI ACNGT 3 cut(s) 132, 138, 664
TaqI TCGA 8 cut(s) 77, 141, 221, 307, 320, 366, 488, 897
TasI AATT 4 cut(s) 244, 634, 803, 899
TatI WGTACW 1 cut(s) 461
TfiI GAWTC 5 cut(s) 101, 233, 490, 683, 894
Tru1I TTAA 2 cut(s) 243, 855
Tru9I TTAA 2 cut(s) 243, 855
TscAI CASTG 1 cut(s) 141
TseFI GTSAC 2 cut(s) 132, 194
TseI GCWGC 2 cut(s) 209, 523
Tsp45I GTSAC 2 cut(s) 132, 194
TspDTI ATGAA 3 cut(s) 276, 409, 575
TspGWI ACGGA 1 cut(s) 893
TspRI CASTG 1 cut(s) 141
VpaK11BI GGWCC 2 cut(s) 128, 547
XapI RAATTY 1 cut(s) 899
XbaI TCTAGA 1 cut(s) 41
XhoI CTCGAG 1 cut(s) 306
XspI CTAG 5 cut(s) 42, 105, 293, 362, 501
ZrmI AGTACT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.