RchiOBHm_Chr3g0448621

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000189
Physical Location & Seq
Reverse (-)
786487 .. 788302
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41605

Sequence Viewer

Length: 1041 bp
ATGCTGACACGAACACACGAAGGAAGAACGAGAACAAGAACAAGAACAAGAACAAGGCACCACGCAGTGAAACAGAGCAGCAAGAAAACCATGCGTAACGATGAAGAAGATGGACGAGGAGGAGGAGGAGGAGGAATCACCTGCGGTGCTGTGACGGTGGGGCTGGATCTGCAGGCTTTGCCGGAGGGATGCATAGCCAACGTCATCTCCCTGACGACTCCTCCAGATGCGTGCCGGCTGTCGTCGGTTTCGAGGAGTTTCAGGTCGGCCGCCGAATCCGACTCCGTTTGGAGCAGGTTCCTGCCGCCGGAGATTCCCACCATCCTGTCTCACTCGGGGCCTCACGCCCCCAGTTGCCGATCCAAATCCAAGGAGTTCTACCTCGCTCTCTGCGACAATCCAGTGCTCATTGACGATGGCAAAATGAGCTTTTCACTAGACAAATGGAGTGGGAAAAAATGTTACATGGTATCTGCAAGGGACCTTGTGATTGTTTGGGCTGATACTCCTAACTATTGGAAGTGGATTTCTCTGCCCGAGTCCAGATTTGAGGAGGTTGCTGAGCTTGTTGGTGTTTGCTGGCTTGAAATCCGTGGCAAAATTGACACTCGCTTGCTGTCCCCATCGACTGTGTACAAAGCTTACCTTGTGTTCAAGTCAACTGCAGCGGCTTATGGATTTGACTACCAACCTGCCGAGGTCAGTGTAGGTCTGGTTGGAGGAGAAGAACCCACAAAGAACCCAGTGTTTCTTGTCGCCGATAGTGGCCGGACTCAGAGCTCCCAGATTGTGCCAAGGCGTCTTGGCCTACACCTGAGAAACCGCATTTTGGTGCAAGTATCCCAGCCCAGAGAGACCAATGACAATCAATACCCGAAGGAAAGAGCAGACGGGTGGTTGGAGATTGAGCTGGGTGAGTTCTTCTGTGAAGGAGGAGTAGATGGGGAGTTGGAAATGACTTGTTTGGAAACCAGCGGTGGTCAGTGGAAGGGTGGTCTTATTGTTCAAGGCATTGAGGTCAGGCCAAAGAGGAAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0030246 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

346

Amino Acids

38.3

Weight (kDa)

8.32

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 58 - 100 1.4e-06 F-box-like
F-box PF00646 58 - 98 1.9e-06 F-box domain
PP2 PF14299 152 - 342 3e-58 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 149
Acc36I ACCTGC 3 cut(s) 149, 285, 700
AccB1I GGYRCC 1 cut(s) 57
AciI CCGC 6 cut(s) 144, 270, 305, 668, 823, 975
AclWI GGATC 2 cut(s) 174, 354
AcoI YGGCCR 2 cut(s) 267, 766
AcyI GRCGYC 1 cut(s) 799
AdeI CACNNNGTG 1 cut(s) 67
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 2 cut(s) 307, 829
AgsI TTSAA 3 cut(s) 587, 655, 1007
AluBI AGCT 5 cut(s) 429, 565, 641, 780, 910
AluI AGCT 5 cut(s) 429, 565, 641, 780, 910
Alw21I GWGCWC 2 cut(s) 408, 782
Alw26I GTCTC 2 cut(s) 333, 848
AlwI GGATC 2 cut(s) 174, 354
Ama87I CYCGRG 2 cut(s) 334, 536
AoxI GGCC 5 cut(s) 267, 338, 766, 805, 1022
ApeKI GCWGC 2 cut(s) 78, 665
ArsI GACNNNNNNTTYG 1 cut(s) 1019
AspS9I GGNCC 2 cut(s) 338, 481
AsuHPI GGTGA 2 cut(s) 130, 926
AvaI CYCGRG 2 cut(s) 334, 536
AvaII GGWCC 1 cut(s) 481
BanI GGYRCC 1 cut(s) 57
BanII GRGCYC 1 cut(s) 782
Bbv12I GWGCWC 2 cut(s) 408, 782
BbvI GCAGC 2 cut(s) 90, 677
BccI CCATC 5 cut(s) 104, 329, 410, 631, 935
BciVI GTATCC 1 cut(s) 850
BcoDI GTCTC 2 cut(s) 333, 848
BfaI CTAG 2 cut(s) 437, 1039
BfmI CTRYAG 2 cut(s) 170, 663
BfuAI ACCTGC 3 cut(s) 149, 285, 700
BfuI GTATCC 1 cut(s) 850
BisI GCNGC 5 cut(s) 79, 270, 305, 666, 669
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 5 cut(s) 80, 271, 306, 667, 670
Bme18I GGWCC 1 cut(s) 481
BmeT110I CYCGRG 2 cut(s) 334, 536
BmgT120I GGNCC 2 cut(s) 338, 481
BmiI GGNNCC 4 cut(s) 59, 299, 339, 482
BmrI ACTGGG 2 cut(s) 345, 737
BmsI GCATC 2 cut(s) 179, 217
BmuI ACTGGG 2 cut(s) 345, 737
BpmI CTGGAG 1 cut(s) 207
Bpu1102I GCTNAGC 1 cut(s) 561
BsaBI GATNNNNATC 1 cut(s) 364
BsaHI GRCGYC 1 cut(s) 799
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 4 cut(s) 369, 592, 696, 794
BsaXI ACNNNNNCTCC 4 cut(s) 191, 205, 221, 235
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 829
Bse118I RCCGGY 1 cut(s) 234
Bse1I ACTGG 3 cut(s) 351, 401, 743
Bse8I GATNNNNATC 1 cut(s) 364
BseDI CCNNGG 4 cut(s) 369, 592, 696, 794
BseGI GGATG 2 cut(s) 194, 321
BseJI GATNNNNATC 1 cut(s) 364
BseLI CCNNNNNNNGG 2 cut(s) 307, 829
BseMII CTCAG 3 cut(s) 552, 788, 806
BseNI ACTGG 3 cut(s) 351, 401, 743
BseX3I CGGCCG 1 cut(s) 267
BseXI GCAGC 2 cut(s) 90, 677
BseYI CCCAGC 2 cut(s) 843, 910
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 5 cut(s) 269, 340, 768, 807, 1024
BshNI GGYRCC 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 270
BsiHKAI GWGCWC 2 cut(s) 408, 782
BsiHKCI CYCGRG 2 cut(s) 334, 536
BsiSI CCGG 4 cut(s) 182, 235, 308, 769
BslFI GGGAC 2 cut(s) 494, 604
BslI CCNNNNNNNGG 2 cut(s) 307, 829
BsmAI GTCTC 2 cut(s) 333, 848
BsmFI GGGAC 2 cut(s) 494, 604
BsnI GGCC 5 cut(s) 269, 340, 768, 807, 1024
Bso31I GGTCTC 1 cut(s) 848
BsoBI CYCGRG 2 cut(s) 334, 536
Bsp1286I GDGCHC 2 cut(s) 408, 782
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 2 cut(s) 166, 359
Bsp1720I GCTNAGC 1 cut(s) 561
BspACI CCGC 6 cut(s) 144, 270, 305, 668, 823, 975
BspANI GGCC 5 cut(s) 269, 340, 768, 807, 1024
BspCNI CTCAG 3 cut(s) 553, 787, 807
BspLI GGNNCC 4 cut(s) 59, 299, 339, 482
BspMAI CTGCAG 2 cut(s) 174, 667
BspMI ACCTGC 3 cut(s) 149, 285, 700
BspPI GGATC 2 cut(s) 174, 354
BspT107I GGYRCC 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 848
BsrFI RCCGGY 1 cut(s) 234
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 3 cut(s) 351, 401, 743
BssAI RCCGGY 1 cut(s) 234
BssECI CCNNGG 4 cut(s) 369, 592, 696, 794
BssMI GATC 2 cut(s) 166, 359
BssNI GRCGYC 1 cut(s) 799
BssT1I CCWWGG 2 cut(s) 369, 794
Bst4CI ACNGT 2 cut(s) 157, 631
BstACI GRCGYC 1 cut(s) 799
BstAPI GCANNNNNTGC 1 cut(s) 178
BstAUI TGTACA 1 cut(s) 633
BstC8I GCNNGC 5 cut(s) 174, 232, 236, 581, 614
BstDEI CTNAG 3 cut(s) 561, 774, 815
BstDSI CCRYGG 1 cut(s) 592
BstF5I GGATG 2 cut(s) 194, 321
BstKTI GATC 2 cut(s) 169, 362
BstMAI GTCTC 2 cut(s) 333, 848
BstMBI GATC 2 cut(s) 166, 359
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 3 cut(s) 169, 178, 426
BstSFI CTRYAG 2 cut(s) 170, 663
BstV1I GCAGC 2 cut(s) 90, 677
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BstZI CGGCCG 1 cut(s) 267
BsuI GTATCC 1 cut(s) 850
BsuRI GGCC 5 cut(s) 269, 340, 768, 807, 1024
BtgI CCRYGG 1 cut(s) 592
BtsCI GGATG 2 cut(s) 194, 321
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 5 cut(s) 72, 408, 709, 750, 989
BveI ACCTGC 3 cut(s) 149, 285, 700
Cac8I GCNNGC 5 cut(s) 174, 232, 236, 581, 614
Cfr10I RCCGGY 1 cut(s) 234
Cfr13I GGNCC 2 cut(s) 338, 481
CseI GACGC 1 cut(s) 788
Csp6I GTAC 1 cut(s) 634
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CviAII CATG 2 cut(s) 91, 466
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 3 cut(s) 561, 774, 815
DpnI GATC 2 cut(s) 168, 361
DpnII GATC 2 cut(s) 166, 359
DraIII CACNNNGTG 1 cut(s) 67
EaeI YGGCCR 2 cut(s) 267, 766
EagI CGGCCG 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 780
EclXI CGGCCG 1 cut(s) 267
Eco130I CCWWGG 2 cut(s) 369, 794
Eco24I GRGCYC 1 cut(s) 782
Eco31I GGTCTC 1 cut(s) 848
Eco47I GGWCC 1 cut(s) 481
Eco52I CGGCCG 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 780
Eco88I CYCGRG 2 cut(s) 334, 536
EcoICRI GAGCTC 1 cut(s) 780
EcoO109I RGGNCCY 2 cut(s) 338, 481
EcoT14I CCWWGG 2 cut(s) 369, 794
EcoT22I ATGCAT 1 cut(s) 194
EcoT38I GRGCYC 1 cut(s) 782
ErhI CCWWGG 2 cut(s) 369, 794
FaeI CATG 2 cut(s) 94, 469
FaiI YATR 4 cut(s) 92, 194, 467, 675
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 2 cut(s) 494, 604
FatI CATG 2 cut(s) 90, 465
Fnu4HI GCNGC 5 cut(s) 79, 270, 305, 666, 669
FokI GGATG 2 cut(s) 201, 308
FriOI GRGCYC 1 cut(s) 782
Fsp4HI GCNGC 5 cut(s) 79, 270, 305, 666, 669
FspBI CTAG 2 cut(s) 437, 1039
GluI GCNGC 5 cut(s) 79, 270, 305, 666, 669
GsaI CCCAGC 2 cut(s) 847, 914
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 5 cut(s) 269, 340, 768, 807, 1024
HapII CCGG 4 cut(s) 182, 235, 308, 769
HgaI GACGC 1 cut(s) 788
Hin1I GRCGYC 1 cut(s) 799
Hin1II CATG 2 cut(s) 94, 469
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HindIII AAGCTT 1 cut(s) 639
HinfI GANTC 7 cut(s) 135, 217, 275, 281, 313, 539, 772
HpaII CCGG 4 cut(s) 182, 235, 308, 769
HphI GGTGA 2 cut(s) 130, 926
Hpy166II GTNNAC 2 cut(s) 634, 660
Hpy188I TCNGA 2 cut(s) 280, 777
Hpy188III TCNNGA 2 cut(s) 224, 543
Hpy8I GTNNAC 2 cut(s) 634, 660
Hpy99I CGWCG 1 cut(s) 247
HpyAV CCTTC 4 cut(s) 14, 871, 923, 982
HpyCH4III ACNGT 2 cut(s) 157, 631
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 5 cut(s) 172, 192, 476, 665, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 178, 426
HpyF3I CTNAG 3 cut(s) 561, 774, 815
HpySE526I ACGT 1 cut(s) 201
Hsp92I GRCGYC 1 cut(s) 799
Hsp92II CATG 2 cut(s) 94, 469
KroI GCCGGC 1 cut(s) 234
KroNI GCCGGC 1 cut(s) 236
Kzo9I GATC 2 cut(s) 166, 359
LmnI GCTCC 2 cut(s) 291, 785
Lsp1109I GCAGC 2 cut(s) 90, 677
LweI GCATC 2 cut(s) 179, 217
MaeI CTAG 2 cut(s) 437, 1039
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 3 cut(s) 95, 151, 461
MalI GATC 2 cut(s) 168, 361
MboI GATC 2 cut(s) 166, 359
MboII GAAGA 5 cut(s) 36, 116, 119, 737, 913
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 2 cut(s) 408, 782
MluCI AATT 1 cut(s) 600
MlyI GAGTC 4 cut(s) 211, 275, 548, 766
MmeI TCCRAC 4 cut(s) 303, 697, 879, 930
Mph1103I ATGCAT 1 cut(s) 194
MroNI GCCGGC 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 830
MspA1I CMGCKG 2 cut(s) 668, 975
MspI CCGG 4 cut(s) 182, 235, 308, 769
MwoI GCNNNNNNNGC 3 cut(s) 169, 178, 426
NaeI GCCGGC 1 cut(s) 236
NdeII GATC 2 cut(s) 166, 359
NgoMIV GCCGGC 1 cut(s) 234
NlaIII CATG 2 cut(s) 94, 469
NlaIV GGNNCC 4 cut(s) 59, 299, 339, 482
NmeAIII GCCGAG 1 cut(s) 721
NmuCI GTSAC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 194
PaqCI CACCTGC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 2 cut(s) 248, 390
PdiI GCCGGC 1 cut(s) 236
PfeI GAWTC 3 cut(s) 135, 275, 313
PkrI GCNGC 5 cut(s) 80, 271, 306, 667, 670
PleI GAGTC 4 cut(s) 211, 275, 547, 766
PpsI GAGTC 4 cut(s) 211, 275, 547, 766
PpuMI RGGWCCY 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 782
Psp5II RGGWCCY 1 cut(s) 481
PspFI CCCAGC 2 cut(s) 843, 910
PspN4I GGNNCC 4 cut(s) 59, 299, 339, 482
PspPI GGNCC 2 cut(s) 338, 481
PspPPI RGGWCCY 1 cut(s) 481
PstI CTGCAG 2 cut(s) 174, 667
PsuI RGATCY 1 cut(s) 166
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 830
SacI GAGCTC 1 cut(s) 782
SatI GCNGC 5 cut(s) 79, 270, 305, 666, 669
Sau3AI GATC 2 cut(s) 166, 359
Sau96I GGNCC 2 cut(s) 338, 481
SchI GAGTC 4 cut(s) 211, 275, 548, 766
SduI GDGCHC 2 cut(s) 408, 782
SfaNI GCATC 2 cut(s) 179, 217
SfcI CTRYAG 2 cut(s) 170, 663
SinI GGWCC 1 cut(s) 481
SmiMI CAYNNNNRTG 1 cut(s) 830
Sse9I AATT 1 cut(s) 600
SsiI CCGC 6 cut(s) 144, 270, 305, 668, 823, 975
SspMI CTAG 2 cut(s) 437, 1039
SstI GAGCTC 1 cut(s) 782
StyI CCWWGG 2 cut(s) 369, 794
TaaI ACNGT 2 cut(s) 157, 631
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 251, 626
TasI AATT 1 cut(s) 600
TatI WGTACW 1 cut(s) 633
TauI GCSGC 3 cut(s) 272, 307, 671
TfiI GAWTC 3 cut(s) 135, 275, 313
TscAI CASTG 5 cut(s) 72, 408, 709, 750, 989
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 2 cut(s) 78, 665
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 1 cut(s) 117
TspGWI ACGGA 2 cut(s) 274, 581
TspRI CASTG 5 cut(s) 72, 408, 709, 750, 989
VpaK11BI GGWCC 1 cut(s) 481
XspI CTAG 2 cut(s) 437, 1039
Zsp2I ATGCAT 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.