pycom12g23410

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000189
Physical Location & Seq
Forward (+)
24013407 .. 24020546
7140 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23410.1

Sequence Viewer

Length: 795 bp
ATGGATCTGCAGGCCTTGCCAGAAGGCTGCATAGCCACCGTTATCTCGCTGACCACGCCTCGAGACGCGGCCACCATGTCCTCGGTTTCGAGGAGTTTCAGGTCGGCAACGGAATCCGACTCCGTTTGGGGCAGGTTCCTTCCCCCCGATATCCACACTATCCTGTCCTCCTCGTCACCCTTCATGTCCGTACAGCCGCTGCATGTGTCTCCCTCCTCCGCTTCCAAAACCAAGGCGCTTTACCTCGCTCTGTGCGACAACCCAGTTCTCATCGAACAGGGCAAGTTGAGCTTTTCACTGGACAAATGGAGTGGGAAGAAATGCTATATGATTGCTGCAAGGGCCCTTTCCATCGTTTGGGCCAACACTCCTCAGTACTGGAAATGGATTTCTATCCCCGACTCAAGGTTTGAGGAGGTGGCAGAGCTTGTGGATGTGTGTTGGCTTGAAATCCATGGCAAAATTGAGACACGGATGCTGTCCCCATCCACCCTTTACAAAGCTTATCTTGTATTCAAGACGACTGCAGAGGCTTATGGACTCGAGCTCCACGCTGCGGAGGTCACAGTGGGTTTAATTGGGGAGATCAATGAGGCCAAAGACCAGCCCAGGGAGATCAATGAGGCCCAGTATCCGAAAGAGCGCAACGACGGGTGGCTGGAGATAGAGATGGGGGAGTTCTTCTGTGAAGAGGGTGAAGGTGGGGAGTTGGAGATGAGTTGCTTGGAGACAAATGGTCACTGGAAGAGCGGCCTCATTGTTCAAGGGATTGAGGTCAGGCCCAAGAGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0030246 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

265

Amino Acids

29.48

Weight (kDa)

5.02

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 106 - 261 1.6e-57 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 123
AccB7I CCANNNNNTGG 1 cut(s) 357
AccBSI CCGCTC 1 cut(s) 750
AccII CGCG 1 cut(s) 68
AciI CCGC 5 cut(s) 68, 197, 219, 557, 750
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 69
AfaI GTAC 2 cut(s) 192, 377
AfiI CCNNNNNNNGG 4 cut(s) 357, 405, 556, 610
AgsI TTSAA 3 cut(s) 449, 517, 764
AhdI GACNNNNNGTC 1 cut(s) 735
AjnI CCWGG 1 cut(s) 608
AluBI AGCT 4 cut(s) 291, 427, 503, 547
AluI AGCT 4 cut(s) 291, 427, 503, 547
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 4 cut(s) 57, 213, 461, 722
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 2 cut(s) 60, 542
AoxI GGCC 8 cut(s) 12, 69, 342, 360, 594, 624, 751, 779
ApaI GGGCCC 1 cut(s) 346
ApeKI GCWGC 4 cut(s) 27, 199, 335, 554
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspLEI GCGC 2 cut(s) 238, 645
AspS9I GGNCC 5 cut(s) 342, 343, 360, 625, 780
AsuHPI GGTGA 2 cut(s) 168, 707
AvaI CYCGRG 2 cut(s) 60, 542
BaeGI GKGCMC 1 cut(s) 346
BanII GRGCYC 2 cut(s) 346, 549
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 14, 186, 322, 541
BccI CCATC 3 cut(s) 359, 493, 664
BciT130I CCWGG 1 cut(s) 610
BciVI GTATCC 1 cut(s) 642
BcoDI GTCTC 4 cut(s) 57, 213, 461, 722
BfmI CTRYAG 2 cut(s) 8, 525
BfoI RGCGCY 1 cut(s) 239
BfuAI ACCTGC 1 cut(s) 123
BfuI GTATCC 1 cut(s) 642
BisI GCNGC 7 cut(s) 28, 69, 197, 200, 336, 555, 751
BlsI GCNGC 7 cut(s) 29, 70, 198, 201, 337, 556, 752
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 1 cut(s) 610
BmeRI GACNNNNNGTC 1 cut(s) 735
BmeT110I CYCGRG 2 cut(s) 60, 542
BmgT120I GGNCC 5 cut(s) 342, 343, 360, 625, 780
BmiI GGNNCC 2 cut(s) 137, 344
BmrFI CCNGG 1 cut(s) 610
BmrI ACTGGG 2 cut(s) 257, 622
BmsI GCATC 1 cut(s) 465
BmuI ACTGGG 2 cut(s) 257, 622
BpmI CTGGAG 1 cut(s) 680
BpuEI CTTGAG 1 cut(s) 388
BsaBI GATNNNNATC 1 cut(s) 392
BsaJI CCNNGG 5 cut(s) 81, 231, 454, 608, 609
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 4 cut(s) 357, 405, 556, 610
Bse1I ACTGG 5 cut(s) 263, 303, 383, 628, 746
Bse8I GATNNNNATC 1 cut(s) 392
BseBI CCWGG 1 cut(s) 610
BseDI CCNNGG 5 cut(s) 81, 231, 454, 608, 609
BseGI GGATG 4 cut(s) 439, 480, 485, 795
BseJI GATNNNNATC 1 cut(s) 392
BseLI CCNNNNNNNGG 4 cut(s) 357, 405, 556, 610
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 5 cut(s) 263, 303, 383, 628, 746
BseRI GAGGAG 5 cut(s) 106, 160, 205, 360, 428
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 4 cut(s) 14, 186, 322, 541
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 8 cut(s) 14, 71, 344, 362, 596, 626, 753, 781
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 2 cut(s) 60, 542
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 4 cut(s) 357, 405, 556, 610
BsmAI GTCTC 4 cut(s) 57, 213, 461, 722
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 8 cut(s) 14, 71, 344, 362, 596, 626, 753, 781
BsoBI CYCGRG 2 cut(s) 60, 542
Bsp120I GGGCCC 1 cut(s) 342
Bsp1286I GDGCHC 2 cut(s) 346, 549
Bsp143I GATC 3 cut(s) 4, 585, 615
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 5 cut(s) 68, 197, 219, 557, 750
BspANI GGCC 8 cut(s) 14, 71, 344, 362, 596, 626, 753, 781
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 2 cut(s) 137, 344
BspMAI CTGCAG 2 cut(s) 12, 529
BspMI ACCTGC 1 cut(s) 123
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 740
BsrBI CCGCTC 1 cut(s) 750
BsrI ACTGG 5 cut(s) 263, 303, 383, 628, 746
BssECI CCNNGG 5 cut(s) 81, 231, 454, 608, 609
BssMI GATC 3 cut(s) 4, 585, 615
BssT1I CCWWGG 2 cut(s) 231, 454
Bst2UI CCWGG 1 cut(s) 610
Bst4CI ACNGT 2 cut(s) 40, 568
Bst6I CTCTTC 2 cut(s) 684, 740
BstAPI GCANNNNNTGC 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 1 cut(s) 372
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 4 cut(s) 439, 480, 485, 795
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 2 cut(s) 238, 645
BstKTI GATC 3 cut(s) 7, 588, 618
BstMAI GTCTC 4 cut(s) 57, 213, 461, 722
BstMBI GATC 3 cut(s) 4, 585, 615
BstMWI GCNNNNNNNGC 4 cut(s) 16, 55, 288, 341
BstNI CCWGG 1 cut(s) 610
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 608
BstSFI CTRYAG 2 cut(s) 8, 525
BstSLI GKGCMC 1 cut(s) 346
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 4 cut(s) 14, 186, 322, 541
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 642
BsuRI GGCC 8 cut(s) 14, 71, 344, 362, 596, 626, 753, 781
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 4 cut(s) 439, 480, 485, 795
BtsIMutI CAGTG 3 cut(s) 296, 573, 739
BveI ACCTGC 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 12
CaiI CAGNNNCTG 1 cut(s) 199
CfoI GCGC 2 cut(s) 238, 645
Cfr13I GGNCC 5 cut(s) 342, 343, 360, 625, 780
CseI GACGC 1 cut(s) 74
Csp6I GTAC 2 cut(s) 191, 376
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 4 cut(s) 76, 184, 203, 455
CviQI GTAC 2 cut(s) 191, 376
DdeI CTNAG 1 cut(s) 372
DpnI GATC 3 cut(s) 6, 587, 617
DpnII GATC 3 cut(s) 4, 585, 615
DriI GACNNNNNGTC 1 cut(s) 735
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 684, 740
Eam1105I GACNNNNNGTC 1 cut(s) 735
EarI CTCTTC 2 cut(s) 684, 740
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 231, 454
Eco147I AGGCCT 1 cut(s) 14
Eco24I GRGCYC 2 cut(s) 346, 549
Eco32I GATATC 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 547
Eco88I CYCGRG 2 cut(s) 60, 542
EcoICRI GAGCTC 1 cut(s) 547
EcoO109I RGGNCCY 2 cut(s) 342, 343
EcoRII CCWGG 1 cut(s) 608
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 2 cut(s) 231, 454
EcoT38I GRGCYC 2 cut(s) 346, 549
ErhI CCWWGG 2 cut(s) 231, 454
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 4 cut(s) 79, 187, 206, 458
FaiI YATR 8 cut(s) 32, 77, 185, 204, 327, 329, 456, 537
FalI AAGNNNNNCTT 4 cut(s) 275, 307, 492, 524
FaqI GGGAC 1 cut(s) 466
FatI CATG 4 cut(s) 75, 183, 202, 454
Fnu4HI GCNGC 7 cut(s) 28, 69, 197, 200, 336, 555, 751
FokI GGATG 3 cut(s) 446, 472, 487
FriOI GRGCYC 2 cut(s) 346, 549
Fsp4HI GCNGC 7 cut(s) 28, 69, 197, 200, 336, 555, 751
GlaI GCGC 2 cut(s) 237, 644
GluI GCNGC 7 cut(s) 28, 69, 197, 200, 336, 555, 751
GsuI CTGGAG 1 cut(s) 680
HaeII RGCGCY 1 cut(s) 239
HaeIII GGCC 8 cut(s) 14, 71, 344, 362, 596, 626, 753, 781
HgaI GACGC 1 cut(s) 74
HhaI GCGC 2 cut(s) 238, 645
Hin1II CATG 4 cut(s) 79, 187, 206, 458
Hin6I GCGC 2 cut(s) 236, 643
HinP1I GCGC 2 cut(s) 236, 643
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 4 cut(s) 113, 119, 401, 540
HphI GGTGA 2 cut(s) 168, 707
Hpy188I TCNGA 2 cut(s) 118, 636
Hpy188III TCNNGA 2 cut(s) 62, 517
Hpy99I CGWCG 1 cut(s) 653
HpyAV CCTTC 4 cut(s) 17, 149, 190, 692
HpyCH4III ACNGT 2 cut(s) 40, 568
HpyCH4V TGCA 5 cut(s) 10, 30, 202, 338, 527
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 55, 288, 341
HpyF3I CTNAG 1 cut(s) 372
Hsp92II CATG 4 cut(s) 79, 187, 206, 458
HspAI GCGC 2 cut(s) 236, 643
Kzo9I GATC 3 cut(s) 4, 585, 615
LguI GCTCTTC 1 cut(s) 740
LmnI GCTCC 1 cut(s) 552
Lsp1109I GCAGC 4 cut(s) 14, 186, 322, 541
LweI GCATC 1 cut(s) 465
MaeIII GTNAC 3 cut(s) 174, 562, 737
MalI GATC 3 cut(s) 6, 587, 617
MbiI CCGCTC 1 cut(s) 750
MboI GATC 3 cut(s) 4, 585, 615
MboII GAAGA 4 cut(s) 328, 673, 701, 757
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 346, 549
MluCI AATT 2 cut(s) 462, 576
MlyI GAGTC 3 cut(s) 113, 395, 534
MmeI TCCRAC 2 cut(s) 141, 690
MseI TTAA 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 199
MspR9I CCNGG 1 cut(s) 610
MvaI CCWGG 1 cut(s) 610
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 4 cut(s) 16, 55, 288, 341
NcoI CCATGG 1 cut(s) 454
NdeII GATC 3 cut(s) 4, 585, 615
NlaIII CATG 4 cut(s) 79, 187, 206, 458
NlaIV GGNNCC 2 cut(s) 137, 344
NmuCI GTSAC 3 cut(s) 174, 562, 737
NspI RCATGY 1 cut(s) 206
PaeR7I CTCGAG 2 cut(s) 60, 542
PasI CCCWGGG 1 cut(s) 609
PceI AGGCCT 1 cut(s) 14
PciSI GCTCTTC 1 cut(s) 740
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 357
PkrI GCNGC 7 cut(s) 29, 70, 198, 201, 337, 556, 752
PleI GAGTC 3 cut(s) 113, 395, 534
PpsI GAGTC 3 cut(s) 113, 395, 534
Psp124BI GAGCTC 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 608
PspGI CCWGG 1 cut(s) 608
PspN4I GGNNCC 2 cut(s) 137, 344
PspOMI GGGCCC 1 cut(s) 342
PspPI GGNCC 5 cut(s) 342, 343, 360, 625, 780
PspXI VCTCGAGB 1 cut(s) 542
PstI CTGCAG 2 cut(s) 12, 529
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 192, 377
RsaNI GTAC 2 cut(s) 191, 376
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 740
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 7 cut(s) 28, 69, 197, 200, 336, 555, 751
Sau3AI GATC 3 cut(s) 4, 585, 615
Sau96I GGNCC 5 cut(s) 342, 343, 360, 625, 780
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 3 cut(s) 113, 395, 534
ScrFI CCNGG 1 cut(s) 610
SduI GDGCHC 2 cut(s) 346, 549
SfaNI GCATC 1 cut(s) 465
SfcI CTRYAG 2 cut(s) 8, 525
Sfr274I CTCGAG 2 cut(s) 60, 542
SlaI CTCGAG 2 cut(s) 60, 542
SmlI CTYRAG 3 cut(s) 60, 403, 542
SmoI CTYRAG 3 cut(s) 60, 403, 542
Sse9I AATT 2 cut(s) 462, 576
SseBI AGGCCT 1 cut(s) 14
SsiI CCGC 5 cut(s) 68, 197, 219, 557, 750
SstI GAGCTC 1 cut(s) 549
StuI AGGCCT 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 608
StyI CCWWGG 2 cut(s) 231, 454
TaaI ACNGT 2 cut(s) 40, 568
TaqI TCGA 4 cut(s) 61, 89, 273, 543
TasI AATT 2 cut(s) 462, 576
TatI WGTACW 1 cut(s) 375
TauI GCSGC 3 cut(s) 71, 199, 753
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 3 cut(s) 303, 573, 746
TseFI GTSAC 3 cut(s) 174, 562, 737
TseI GCWGC 4 cut(s) 27, 199, 335, 554
Tsp45I GTSAC 3 cut(s) 174, 562, 737
TspDTI ATGAA 1 cut(s) 172
TspGWI ACGGA 4 cut(s) 112, 125, 178, 487
TspRI CASTG 3 cut(s) 303, 573, 746
Van91I CCANNNNNTGG 1 cut(s) 357
XceI RCATGY 1 cut(s) 206
XhoI CTCGAG 2 cut(s) 60, 542
ZrmI AGTACT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.