Rroxscaffold_2G00151450

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000189
Physical Location & Seq
Forward (+)
88692947 .. 88693582
636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151450.1

Sequence Viewer

Length: 636 bp
ATGGCTCTGTGCGACAACCCCGTGCTCATTGACGAGGGCTATACGAGCTTTTCACTGGACAAATGGAGTGGGAAGAAGTGCTATATGATAGCTGCAAAGCGCCTTCGGATTGCTTGGGGTGATACTCCTCGATATTGGACATGGAAATCTGTGACTGAGTCCAGGTTTAAGGAGGCGGCCGAGCTTTGTGGTCAAGTATTTTGGCTTGAAATCCGTGGGAGAATTGAAAAGCGCATGCTGTCTCCATCCACGACGTATAAAGCTTATCTGGTGTACAAGTTAACTCAACACACTTATGGACTCAATTGGGCAGCCGTGGTCACAGTTGGTGCAAGAGCTTATGGCTTTGATTATGAATGCCCTGGTTTGAAGGAAATTACTGCAGGCATTAAGAGCACTGCGTTTCTGGCACCCCAAAGAGAAATAGATGGACAGCTCGCAAGAAATAAAAAAATAAAAAAAGCTAAATTTCCAAATGAAGGCAGGGCGGATGGGTGGCTGGAGATGGAGCTGGGTGAGTTTCACTATCAAGGGGACGAAGACGGGGAGTTGGAAATGATTTGTTGTGAGATCAGAGGTTCAAGTAAGAGTGGTCTCCTTGTTCAAGGGATTGAGGTCAGACCAAAAAGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

211

Amino Acids

24.03

Weight (kDa)

8.8

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 25 - 208 1.3e-44 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 409
AciI CCGC 2 cut(s) 176, 488
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 1 cut(s) 467
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 5 cut(s) 209, 227, 370, 582, 605
AjnI CCWGG 2 cut(s) 161, 361
AluBI AGCT 9 cut(s) 48, 92, 184, 263, 338, 436, 464, 511, 633
AluI AGCT 9 cut(s) 48, 92, 184, 263, 338, 436, 464, 511, 633
Alw21I GWGCWC 2 cut(s) 27, 398
Alw26I GTCTC 2 cut(s) 246, 599
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 92, 311
ApoI RAATTY 1 cut(s) 467
AspLEI GCGC 2 cut(s) 102, 234
AsuHPI GGTGA 2 cut(s) 131, 527
BanI GGYRCC 1 cut(s) 409
BbsI GAAGAC 1 cut(s) 546
Bbv12I GWGCWC 2 cut(s) 27, 398
BbvI GCAGC 2 cut(s) 79, 323
BccI CCATC 4 cut(s) 253, 422, 485, 499
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 2 cut(s) 163, 363
BcoDI GTCTC 2 cut(s) 246, 599
BfmI CTRYAG 1 cut(s) 381
BfoI RGCGCY 1 cut(s) 103
BisI GCNGC 3 cut(s) 93, 177, 312
BlsI GCNGC 3 cut(s) 94, 178, 313
Bme1390I CCNGG 2 cut(s) 163, 363
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 2 cut(s) 163, 363
BpiI GAAGAC 1 cut(s) 546
BpmI CTGGAG 1 cut(s) 521
BsaI GGTCTC 1 cut(s) 599
BsaJI CCNNGG 3 cut(s) 214, 315, 361
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 163, 363
BseDI CCNNGG 3 cut(s) 214, 315, 361
BseGI GGATG 2 cut(s) 245, 496
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 60
BseRI GAGGAG 1 cut(s) 117
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 2 cut(s) 79, 323
BseYI CCCAGC 1 cut(s) 511
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 1 cut(s) 179
BshNI GGYRCC 1 cut(s) 409
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 27, 398
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 2 cut(s) 246, 599
BsmFI GGGAC 1 cut(s) 548
BsmI GAATGC 1 cut(s) 362
BsnI GGCC 1 cut(s) 179
Bso31I GGTCTC 1 cut(s) 599
Bsp1286I GDGCHC 2 cut(s) 27, 398
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 1 cut(s) 570
BspACI CCGC 2 cut(s) 176, 488
BspANI GGCC 1 cut(s) 179
BspCNI CTCAG 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 411
BspMAI CTGCAG 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 409
BspTNI GGTCTC 1 cut(s) 599
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 3 cut(s) 214, 315, 361
BssMI GATC 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 163, 363
Bst4CI ACNGT 1 cut(s) 325
BstAUI TGTACA 1 cut(s) 273
BstC8I GCNNGC 3 cut(s) 236, 385, 438
BstDEI CTNAG 1 cut(s) 156
BstDSI CCRYGG 2 cut(s) 214, 315
BstF5I GGATG 2 cut(s) 245, 496
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 2 cut(s) 102, 234
BstKTI GATC 1 cut(s) 573
BstMAI GTCTC 2 cut(s) 246, 599
BstMBI GATC 1 cut(s) 570
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 3 cut(s) 45, 393, 407
BstNI CCWGG 2 cut(s) 163, 363
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 161, 361
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 2 cut(s) 79, 323
BstV2I GAAGAC 1 cut(s) 546
BstZI CGGCCG 1 cut(s) 177
BsuRI GGCC 1 cut(s) 179
BtgI CCRYGG 2 cut(s) 214, 315
BtsCI GGATG 2 cut(s) 245, 496
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 53, 396
Cac8I GCNNGC 3 cut(s) 236, 385, 438
CfoI GCGC 2 cut(s) 102, 234
Csp6I GTAC 1 cut(s) 274
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 2 cut(s) 141, 235
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 156
DpnI GATC 1 cut(s) 572
DpnII GATC 1 cut(s) 570
EaeI YGGCCR 1 cut(s) 177
EagI CGGCCG 1 cut(s) 177
EciI GGCGGA 1 cut(s) 503
EclXI CGGCCG 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 599
Eco52I CGGCCG 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 161, 361
FaeI CATG 2 cut(s) 144, 238
FaiI YATR 9 cut(s) 42, 84, 86, 142, 236, 258, 297, 342, 354
FaqI GGGAC 1 cut(s) 548
FatI CATG 2 cut(s) 140, 234
Fnu4HI GCNGC 3 cut(s) 93, 177, 312
FokI GGATG 2 cut(s) 232, 503
Fsp4HI GCNGC 3 cut(s) 93, 177, 312
GlaI GCGC 2 cut(s) 101, 233
GluI GCNGC 3 cut(s) 93, 177, 312
GsaI CCCAGC 1 cut(s) 515
GsuI CTGGAG 1 cut(s) 521
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 1 cut(s) 179
HhaI GCGC 2 cut(s) 102, 234
Hin1II CATG 2 cut(s) 144, 238
Hin6I GCGC 2 cut(s) 100, 232
HinP1I GCGC 2 cut(s) 100, 232
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 2 cut(s) 158, 300
HpaI GTTAAC 1 cut(s) 282
HphI GGTGA 2 cut(s) 131, 527
Hpy166II GTNNAC 2 cut(s) 274, 282
Hpy188I TCNGA 3 cut(s) 108, 575, 620
Hpy8I GTNNAC 2 cut(s) 274, 282
Hpy99I CGWCG 1 cut(s) 256
HpyAV CCTTC 3 cut(s) 113, 364, 473
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 3 cut(s) 95, 332, 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 393, 407
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 2 cut(s) 144, 238
HspAI GCGC 2 cut(s) 100, 232
KspAI GTTAAC 1 cut(s) 282
Kzo9I GATC 1 cut(s) 570
LmnI GCTCC 2 cut(s) 508, 630
Lsp1109I GCAGC 2 cut(s) 79, 323
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 2 cut(s) 151, 319
MalI GATC 1 cut(s) 572
MboI GATC 1 cut(s) 570
MboII GAAGA 2 cut(s) 85, 551
MfeI CAATTG 1 cut(s) 304
MhlI GDGCHC 2 cut(s) 27, 398
MluCI AATT 4 cut(s) 222, 304, 375, 467
MlyI GAGTC 2 cut(s) 167, 294
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 5 cut(s) 28, 138, 166, 569, 607
MseI TTAA 3 cut(s) 168, 281, 390
MslI CAYNNNNRTG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 163, 363
MunI CAATTG 1 cut(s) 304
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 2 cut(s) 163, 363
MwoI GCNNNNNNNGC 3 cut(s) 45, 393, 407
NdeII GATC 1 cut(s) 570
NlaIII CATG 2 cut(s) 144, 238
NlaIV GGNNCC 1 cut(s) 411
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 151, 319
NspI RCATGY 1 cut(s) 238
PaeI GCATGC 1 cut(s) 238
PctI GAATGC 1 cut(s) 362
PflFI GACNNNGTC 1 cut(s) 157
PkrI GCNGC 3 cut(s) 94, 178, 313
PleI GAGTC 2 cut(s) 166, 294
PpsI GAGTC 2 cut(s) 166, 294
Psp6I CCWGG 2 cut(s) 161, 361
PspFI CCCAGC 1 cut(s) 511
PspGI CCWGG 2 cut(s) 161, 361
PspN4I GGNNCC 1 cut(s) 411
PstI CTGCAG 1 cut(s) 385
PsyI GACNNNGTC 1 cut(s) 157
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 3 cut(s) 168, 281, 390
SatI GCNGC 3 cut(s) 93, 177, 312
Sau3AI GATC 1 cut(s) 570
SchI GAGTC 2 cut(s) 167, 294
ScrFI CCNGG 2 cut(s) 163, 363
SduI GDGCHC 2 cut(s) 27, 398
SfcI CTRYAG 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 294
SphI GCATGC 1 cut(s) 238
Sse9I AATT 4 cut(s) 222, 304, 375, 467
SsiI CCGC 2 cut(s) 176, 488
StyD4I CCNGG 2 cut(s) 161, 361
TaaI ACNGT 1 cut(s) 325
TaiI ACGT 1 cut(s) 257
TaqI TCGA 1 cut(s) 130
TasI AATT 4 cut(s) 222, 304, 375, 467
TatI WGTACW 1 cut(s) 273
TauI GCSGC 1 cut(s) 179
Tru1I TTAA 3 cut(s) 168, 281, 390
Tru9I TTAA 3 cut(s) 168, 281, 390
TscAI CASTG 2 cut(s) 60, 403
TseFI GTSAC 2 cut(s) 151, 319
TseI GCWGC 2 cut(s) 92, 311
Tsp45I GTSAC 2 cut(s) 151, 319
TspDTI ATGAA 2 cut(s) 369, 492
TspGWI ACGGA 1 cut(s) 203
TspRI CASTG 2 cut(s) 60, 403
Tth111I GACNNNGTC 1 cut(s) 157
XapI RAATTY 1 cut(s) 467
XceI RCATGY 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.