MD04G1239300.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000189
Physical Location & Seq
Forward (+)
31752641 .. 31753981
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1239300.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGTACTCGATGACGATCATCATCACGTTCACATGGATCTGCAGGAGTTGCCCGAAGGATGCATAGCTAAAGTCGTCTCCTTAACGACGCCTCGAGACGCGTGCAGGTTTTCGGCGGTGTCTAAGAGTTTCATGTCGGCCGCTGATTCCGACGCTGTTTGGGACAAGTTCCTTCCCCCTGATATCCCCTCCATCCTATCTCACGCCTCCTCCTCCTCCTCCACCTCCTCCTCCTCCAAGAAGAAGCTTTACCTCACTCTCTGCGAAAACCCTGTCCTCGTTGACGACGGCAAAATGAGTTTTACTCTGGACAAATGGAGTGGGAAAAAATGTTACATGATATCGCCGAAGGACCTTATGATTGCTTGGGGTGGTACTCCTGCCTACTGGAAATGGATTTCTCTTGCCAACTCCAGGTTTGAGGAGGTGGCTGAGCTTGTAGTTGTTTGGTGGCTTGAGATCAATGGCAAAATTGATACACGGATTCTTTCCCCCTCCACTGTCTACAAAGCTTATCTTGTGTTCATGTTAAGTGAAAGGGCTAGAGGATTTGATCGTGACGATCTCTTGGAGGTCAAGGTAGGACTTTTTGGGGAAGAAGAGACTAATAGTAAGCGCACTGTGTTTCCGGGCCAAGAAAGGAGCCTAAAAGTAGGATGGAGGAGACAGAACATTATCGGGAGACCTATCGATGAAACTCAATATCCAAAGAAAAGGCCCGACGGCTGGCTGGAGGTGGAGATTGGAGAGTTTTTATGTCCAGGAGAAGAAGATGGGGGGTTAATGGAGATGGCATGTATGGAGGTTGATAGAATGATGAGAGGCCTTATTGTTCAGGGGATTGAAGTCAGGCCCAAAAGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0030246 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

289

Amino Acids

32.66

Weight (kDa)

5.53

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 55 8.7e-06 F-box domain
PP2 PF14299 109 - 285 9.4e-48 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 96
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 101
AciI CCGC 2 cut(s) 116, 141
AclWI GGATC 1 cut(s) 45
AcoI YGGCCR 1 cut(s) 138
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 2 cut(s) 6, 376
AfiI CCNNNNNNNGG 2 cut(s) 414, 726
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 1 cut(s) 845
AjnI CCWGG 2 cut(s) 413, 760
AluBI AGCT 4 cut(s) 68, 247, 436, 512
AluI AGCT 4 cut(s) 68, 247, 436, 512
Alw26I GTCTC 5 cut(s) 82, 90, 596, 658, 676
AlwI GGATC 1 cut(s) 45
Ama87I CYCGRG 1 cut(s) 93
AoxI GGCC 5 cut(s) 138, 631, 716, 823, 851
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
AspLEI GCGC 1 cut(s) 618
AspS9I GGNCC 4 cut(s) 352, 631, 717, 852
AsuC2I CCSGG 1 cut(s) 630
AvaI CYCGRG 1 cut(s) 93
AvaII GGWCC 1 cut(s) 352
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
BccI CCATC 4 cut(s) 200, 651, 767, 784
BceAI ACGGC 2 cut(s) 304, 739
BcgI CGANNNNNNTGC 2 cut(s) 84, 118
BciT130I CCWGG 2 cut(s) 415, 762
BcnI CCSGG 1 cut(s) 630
BcoDI GTCTC 5 cut(s) 82, 90, 596, 658, 676
BfaI CTAG 1 cut(s) 543
BfmI CTRYAG 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 96
BisI GCNGC 1 cut(s) 141
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 3 cut(s) 415, 630, 762
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 93
BmgT120I GGNCC 4 cut(s) 352, 631, 717, 852
BmiI GGNNCC 1 cut(s) 644
BmrFI CCNGG 3 cut(s) 415, 630, 762
BmsI GCATC 1 cut(s) 50
BplI GAGNNNNNCTC 2 cut(s) 289, 321
BpmI CTGGAG 2 cut(s) 397, 752
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 630
Bsa29I ATCGAT 1 cut(s) 690
BsaBI GATNNNNATC 2 cut(s) 15, 21
BsaHI GRCGYC 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 676
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 414, 726
Bse1I ACTGG 1 cut(s) 392
Bse8I GATNNNNATC 2 cut(s) 15, 21
BseBI CCWGG 2 cut(s) 415, 762
BseCI ATCGAT 1 cut(s) 690
BseGI GGATG 3 cut(s) 65, 192, 662
BseJI GATNNNNATC 2 cut(s) 15, 21
BseLI CCNNNNNNNGG 2 cut(s) 414, 726
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 392
BseRI GAGGAG 9 cut(s) 199, 202, 205, 208, 217, 220, 223, 437, 676
BseX3I CGGCCG 1 cut(s) 138
BsgI GTGCAG 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 5 cut(s) 140, 633, 718, 825, 853
BshVI ATCGAT 1 cut(s) 690
BsiEI CGRYCG 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 1 cut(s) 629
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 414, 726
BsmAI GTCTC 5 cut(s) 82, 90, 596, 658, 676
BsmBI CGTCTC 2 cut(s) 82, 90
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 5 cut(s) 140, 633, 718, 825, 853
Bso31I GGTCTC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 93
Bsp143I GATC 5 cut(s) 16, 37, 459, 553, 562
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 2 cut(s) 116, 141
BspANI GGCC 5 cut(s) 140, 633, 718, 825, 853
BspCNI CTCAG 1 cut(s) 424
BspDI ATCGAT 1 cut(s) 690
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 1 cut(s) 644
BspMAI CTGCAG 1 cut(s) 45
BspMI ACCTGC 1 cut(s) 96
BspPI GGATC 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 676
BsrI ACTGG 1 cut(s) 392
BssMI GATC 5 cut(s) 16, 37, 459, 553, 562
BssNI GRCGYC 1 cut(s) 89
Bst2UI CCWGG 2 cut(s) 415, 762
Bst4CI ACNGT 2 cut(s) 502, 622
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 89
BstAPI GCANNNNNTGC 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 103, 728
BstDEI CTNAG 2 cut(s) 123, 432
BstF5I GGATG 3 cut(s) 65, 192, 662
BstFNI CGCG 1 cut(s) 101
BstHHI GCGC 1 cut(s) 618
BstKTI GATC 5 cut(s) 19, 40, 462, 556, 565
BstMAI GTCTC 5 cut(s) 82, 90, 596, 658, 676
BstMBI GATC 5 cut(s) 16, 37, 459, 553, 562
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNI CCWGG 2 cut(s) 415, 762
BstNSI RCATGY 1 cut(s) 798
BstSCI CCNGG 3 cut(s) 413, 628, 760
BstSFI CTRYAG 1 cut(s) 41
BstUI CGCG 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BstZI CGGCCG 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 690
BsuRI GGCC 5 cut(s) 140, 633, 718, 825, 853
BsuTUI ATCGAT 1 cut(s) 690
BtsCI GGATG 3 cut(s) 65, 192, 662
BtsIMutI CAGTG 2 cut(s) 498, 618
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 2 cut(s) 103, 728
CfoI GCGC 1 cut(s) 618
Cfr13I GGNCC 4 cut(s) 352, 631, 717, 852
ClaI ATCGAT 1 cut(s) 690
CseI GACGC 3 cut(s) 97, 107, 161
Csp6I GTAC 2 cut(s) 5, 375
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CviAII CATG 5 cut(s) 34, 133, 337, 526, 795
CviQI GTAC 2 cut(s) 5, 375
DdeI CTNAG 2 cut(s) 123, 432
DpnI GATC 5 cut(s) 18, 39, 461, 555, 564
DpnII GATC 5 cut(s) 16, 37, 459, 553, 562
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
EclXI CGGCCG 1 cut(s) 138
Eco147I AGGCCT 1 cut(s) 825
Eco31I GGTCTC 1 cut(s) 676
Eco32I GATATC 2 cut(s) 184, 342
Eco47I GGWCC 1 cut(s) 352
Eco52I CGGCCG 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 93
EcoO109I RGGNCCY 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 413, 760
EcoRV GATATC 2 cut(s) 184, 342
EcoT22I ATGCAT 1 cut(s) 65
Esp3I CGTCTC 2 cut(s) 82, 90
FaeI CATG 5 cut(s) 37, 136, 340, 529, 798
FaiI YATR 9 cut(s) 35, 65, 134, 338, 359, 527, 757, 796, 800
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FaqI GGGAC 1 cut(s) 176
FatI CATG 5 cut(s) 33, 132, 336, 525, 794
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 3 cut(s) 72, 179, 669
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 543
GlaI GCGC 1 cut(s) 617
GluI GCNGC 1 cut(s) 141
GsuI CTGGAG 2 cut(s) 397, 752
HaeIII GGCC 5 cut(s) 140, 633, 718, 825, 853
HapII CCGG 1 cut(s) 629
HgaI GACGC 3 cut(s) 97, 107, 161
HhaI GCGC 1 cut(s) 618
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 5 cut(s) 37, 136, 340, 529, 798
Hin6I GCGC 1 cut(s) 616
HinP1I GCGC 1 cut(s) 616
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 2 cut(s) 245, 510
HinfI GANTC 2 cut(s) 146, 484
HpaII CCGG 1 cut(s) 629
Hpy166II GTNNAC 3 cut(s) 31, 283, 505
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 4 cut(s) 95, 308, 557, 679
Hpy8I GTNNAC 3 cut(s) 31, 283, 505
Hpy99I CGWCG 4 cut(s) 91, 155, 290, 725
HpyAV CCTTC 3 cut(s) 50, 182, 343
HpyCH4III ACNGT 2 cut(s) 502, 622
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 3 cut(s) 43, 63, 105
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 2 cut(s) 123, 432
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 5 cut(s) 37, 136, 340, 529, 798
HspAI GCGC 1 cut(s) 616
Kzo9I GATC 5 cut(s) 16, 37, 459, 553, 562
LmnI GCTCC 1 cut(s) 642
LweI GCATC 1 cut(s) 50
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 332, 557
MalI GATC 5 cut(s) 18, 39, 461, 555, 564
MboI GATC 5 cut(s) 16, 37, 459, 553, 562
MboII GAAGA 5 cut(s) 253, 608, 611, 779, 782
MflI RGATCY 1 cut(s) 37
MluCI AATT 1 cut(s) 471
MluI ACGCGT 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 174
Mph1103I ATGCAT 1 cut(s) 65
MseI TTAA 3 cut(s) 83, 530, 782
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 629
MspR9I CCNGG 3 cut(s) 415, 630, 762
MvaI CCWGG 2 cut(s) 415, 762
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 49
NciI CCSGG 1 cut(s) 630
NdeII GATC 5 cut(s) 16, 37, 459, 553, 562
NlaIII CATG 5 cut(s) 37, 136, 340, 529, 798
NlaIV GGNNCC 1 cut(s) 644
NmuCI GTSAC 1 cut(s) 557
NsiI ATGCAT 1 cut(s) 65
NspI RCATGY 1 cut(s) 798
PaeR7I CTCGAG 1 cut(s) 93
PceI AGGCCT 1 cut(s) 825
PfeI GAWTC 2 cut(s) 146, 484
PfoI TCCNGGA 1 cut(s) 760
PkrI GCNGC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 2 cut(s) 413, 760
PspGI CCWGG 2 cut(s) 413, 760
PspN4I GGNNCC 1 cut(s) 644
PspPI GGNCC 4 cut(s) 352, 631, 717, 852
PspPPI RGGWCCY 1 cut(s) 352
PstI CTGCAG 1 cut(s) 45
PsuI RGATCY 1 cut(s) 37
RsaI GTAC 2 cut(s) 6, 376
RsaNI GTAC 2 cut(s) 5, 375
SaqAI TTAA 3 cut(s) 83, 530, 782
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 5 cut(s) 16, 37, 459, 553, 562
Sau96I GGNCC 4 cut(s) 352, 631, 717, 852
ScrFI CCNGG 3 cut(s) 415, 630, 762
SfaNI GCATC 1 cut(s) 50
SfcI CTRYAG 1 cut(s) 41
Sfr274I CTCGAG 1 cut(s) 93
SinI GGWCC 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 93
SmlI CTYRAG 2 cut(s) 93, 455
SmoI CTYRAG 2 cut(s) 93, 455
Sse9I AATT 1 cut(s) 471
SseBI AGGCCT 1 cut(s) 825
SsiI CCGC 2 cut(s) 116, 141
SspMI CTAG 1 cut(s) 543
StuI AGGCCT 1 cut(s) 825
StyD4I CCNGG 3 cut(s) 413, 628, 760
TaaI ACNGT 2 cut(s) 502, 622
TaiI ACGT 1 cut(s) 30
TaqI TCGA 3 cut(s) 9, 94, 690
TasI AATT 1 cut(s) 471
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 2 cut(s) 146, 484
Tru1I TTAA 3 cut(s) 83, 530, 782
Tru9I TTAA 3 cut(s) 83, 530, 782
TscAI CASTG 2 cut(s) 505, 625
TseFI GTSAC 1 cut(s) 557
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 3 cut(s) 121, 514, 708
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 2 cut(s) 505, 625
VpaK11BI GGWCC 1 cut(s) 352
XceI RCATGY 1 cut(s) 798
XhoI CTCGAG 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 1 cut(s) 543
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.