MD12G1255500.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000189
Physical Location & Seq
Forward (+)
32346162 .. 32348292
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1255500.v1.1.491

Sequence Viewer

Length: 927 bp
ATGTCTAAACAAATTACAAGAACCAGAAGAAAACACATGGATCTGCAGGCCTTGCCGGAAGGGTGCATAGCCACCGTTATCTCGCTGACCACGCCTCGAGACGCGGGCACGTTGTCCTCGGTTTCGTGGAGTTTCAGGTCGGCAGCGGATTCCGACGCCGTCTGGGGCAGGTTCCTTCCCCCCGATATCCACACTATCCTGTCATCCTCGTCACCCTCCATGTCCGTACAGCCGCCGCACGTGGGTCCCTCCTCCGCTTCAAAAACGAAGAAGGAGCTTTACCTCGCTCTGTGCGACAACCCAGTTCTCATCGAACAGGGCAAGTTGAGCTTTTCACTGGACAAATGGAGTGGGAAGAAATGCTATATGATTGCTGCAAGGGCCCTTTCCATCGTTTGGGCCGACACTCCTCAGTACTGGAAATGGATTTCTATCCCCGACTCAAGGTTTGAGGAGGTGGCAGAGCTTGTGGATGTGTGTTGGCTTGAAATCCATGGCAGAATTGAGACACGGATGCTGTCCCCATCCACCCTTTACAAAGCTTATCTTGTATTCAAGACAACTGCACAGGCTTATGGATTTGAGCACCGCGCTGCGGAGGTCACAGTGGGTTTAATTGGGGAGCAGCGCACGAATCAAAATGTGTTTCTGGGCGCTCGGAGAGTCCAGACCCGAGGCCAGGGCTATCACATGGCCAGGCCTCGGGGTATTCGCCGAACATACATTCTAGACCAGCCCAGGGAGATCAATGAGGCCCAGTACCCGAAAGAGCGCAACGACGGATGGCTGGAGATAGAGATGGGGGAGTTCTTTTGTGAAGGAGGTGAAGGTGGGGAGTTGGAGATGAGTTGCTTGGAAACAAAGGGTAGACACTGGAAGAGCGGCCTCATTGTTCAAGGGATTGAGATCAGACCCAAAAGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0030246 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

309

Amino Acids

34.84

Weight (kDa)

8.83

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 119 - 305 1e-60 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 159
AccB7I CCANNNNNTGG 1 cut(s) 396
AccBSI CCGCTC 1 cut(s) 882
AccI GTMKAC 1 cut(s) 868
AccII CGCG 2 cut(s) 104, 591
AciI CCGC 8 cut(s) 104, 146, 233, 236, 255, 589, 596, 882
AclWI GGATC 1 cut(s) 48
AcoI YGGCCR 1 cut(s) 693
AcvI CACGTG 1 cut(s) 241
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 3 cut(s) 228, 416, 761
AfiI CCNNNNNNNGG 7 cut(s) 242, 396, 444, 595, 679, 702, 739
AgsI TTSAA 4 cut(s) 261, 488, 556, 896
AjnI CCWGG 3 cut(s) 678, 695, 737
AluBI AGCT 4 cut(s) 277, 330, 466, 542
AluI AGCT 4 cut(s) 277, 330, 466, 542
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 2 cut(s) 93, 500
AlwI GGATC 1 cut(s) 48
Ama87I CYCGRG 3 cut(s) 96, 672, 702
AoxI GGCC 8 cut(s) 48, 381, 399, 676, 693, 698, 753, 883
ApaI GGGCCC 1 cut(s) 385
ApeKI GCWGC 4 cut(s) 143, 374, 593, 625
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
AspLEI GCGC 4 cut(s) 593, 630, 656, 774
AspS9I GGNCC 5 cut(s) 245, 381, 382, 399, 754
AsuHPI GGTGA 2 cut(s) 204, 836
AvaI CYCGRG 3 cut(s) 96, 672, 702
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 2 cut(s) 110, 385
BalI TGGCCA 1 cut(s) 695
BanII GRGCYC 1 cut(s) 385
BarI GAAGNNNNNNTAC 2 cut(s) 263, 295
BbrPI CACGTG 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 155, 361, 580, 637
BccI CCATC 4 cut(s) 398, 532, 777, 793
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 3 cut(s) 680, 697, 739
BcoDI GTCTC 2 cut(s) 93, 500
BfaI CTAG 1 cut(s) 728
BfmI CTRYAG 1 cut(s) 44
BfoI RGCGCY 1 cut(s) 657
BfuAI ACCTGC 1 cut(s) 159
BisI GCNGC 7 cut(s) 144, 233, 236, 375, 594, 626, 883
BlsI GCNGC 7 cut(s) 145, 234, 237, 376, 595, 627, 884
BmcAI AGTACT 1 cut(s) 416
Bme1390I CCNGG 3 cut(s) 680, 697, 739
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 3 cut(s) 96, 672, 702
BmgT120I GGNCC 5 cut(s) 245, 381, 382, 399, 754
BmiI GGNNCC 4 cut(s) 173, 246, 247, 383
BmrFI CCNGG 3 cut(s) 680, 697, 739
BmrI ACTGGG 2 cut(s) 296, 751
BmsI GCATC 1 cut(s) 504
BmuI ACTGGG 2 cut(s) 296, 751
BpmI CTGGAG 1 cut(s) 809
BpuEI CTTGAG 1 cut(s) 427
BsaAI YACGTR 1 cut(s) 241
BsaBI GATNNNNATC 2 cut(s) 431, 905
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 7 cut(s) 117, 493, 673, 679, 701, 737, 738
Bsc4I CCNNNNNNNGG 7 cut(s) 242, 396, 444, 595, 679, 702, 739
Bse1I ACTGG 5 cut(s) 302, 342, 422, 757, 878
Bse8I GATNNNNATC 2 cut(s) 431, 905
BseBI CCWGG 3 cut(s) 680, 697, 739
BseDI CCNNGG 7 cut(s) 117, 493, 673, 679, 701, 737, 738
BseGI GGATG 6 cut(s) 203, 478, 519, 524, 788, 927
BseJI GATNNNNATC 2 cut(s) 431, 905
BseLI CCNNNNNNNGG 7 cut(s) 242, 396, 444, 595, 679, 702, 739
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 5 cut(s) 302, 342, 422, 757, 878
BseRI GAGGAG 3 cut(s) 241, 399, 467
BseSI GKGCMC 2 cut(s) 110, 385
BseXI GCAGC 4 cut(s) 155, 361, 580, 637
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 2 cut(s) 104, 591
BshFI GGCC 8 cut(s) 50, 383, 401, 678, 695, 700, 755, 885
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 3 cut(s) 96, 672, 702
BsiSI CCGG 1 cut(s) 56
BslFI GGGAC 2 cut(s) 231, 505
BslI CCNNNNNNNGG 7 cut(s) 242, 396, 444, 595, 679, 702, 739
BsmAI GTCTC 2 cut(s) 93, 500
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 231, 505
BsnI GGCC 8 cut(s) 50, 383, 401, 678, 695, 700, 755, 885
BsoBI CYCGRG 3 cut(s) 96, 672, 702
Bsp120I GGGCCC 1 cut(s) 381
Bsp1286I GDGCHC 3 cut(s) 110, 385, 588
Bsp143I GATC 3 cut(s) 40, 744, 906
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 8 cut(s) 104, 146, 233, 236, 255, 589, 596, 882
BspANI GGCC 8 cut(s) 50, 383, 401, 678, 695, 700, 755, 885
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 2 cut(s) 104, 591
BspLI GGNNCC 4 cut(s) 173, 246, 247, 383
BspMAI CTGCAG 1 cut(s) 48
BspMI ACCTGC 1 cut(s) 159
BspPI GGATC 1 cut(s) 48
BspQI GCTCTTC 1 cut(s) 872
BsrBI CCGCTC 1 cut(s) 882
BsrI ACTGG 5 cut(s) 302, 342, 422, 757, 878
BssECI CCNNGG 7 cut(s) 117, 493, 673, 679, 701, 737, 738
BssMI GATC 3 cut(s) 40, 744, 906
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 3 cut(s) 680, 697, 739
Bst4CI ACNGT 2 cut(s) 76, 607
Bst6I CTCTTC 1 cut(s) 872
BstACI GRCGYC 1 cut(s) 156
BstAPI GCANNNNNTGC 1 cut(s) 52
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 48, 106
BstDEI CTNAG 1 cut(s) 411
BstDSI CCRYGG 1 cut(s) 493
BstF5I GGATG 6 cut(s) 203, 478, 519, 524, 788, 927
BstFNI CGCG 2 cut(s) 104, 591
BstH2I RGCGCY 1 cut(s) 657
BstHHI GCGC 4 cut(s) 593, 630, 656, 774
BstKTI GATC 3 cut(s) 43, 747, 909
BstMAI GTCTC 2 cut(s) 93, 500
BstMBI GATC 3 cut(s) 40, 744, 906
BstMWI GCNNNNNNNGC 4 cut(s) 52, 91, 327, 380
BstNI CCWGG 3 cut(s) 680, 697, 739
BstSCI CCNGG 3 cut(s) 678, 695, 737
BstSFI CTRYAG 1 cut(s) 44
BstSLI GKGCMC 2 cut(s) 110, 385
BstUI CGCG 2 cut(s) 104, 591
BstV1I GCAGC 4 cut(s) 155, 361, 580, 637
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BsuRI GGCC 8 cut(s) 50, 383, 401, 678, 695, 700, 755, 885
BtgI CCRYGG 1 cut(s) 493
BtsCI GGATG 6 cut(s) 203, 478, 519, 524, 788, 927
BtsIMutI CAGTG 3 cut(s) 335, 612, 871
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 2 cut(s) 48, 106
CfoI GCGC 4 cut(s) 593, 630, 656, 774
Cfr13I GGNCC 5 cut(s) 245, 381, 382, 399, 754
CseI GACGC 2 cut(s) 110, 164
Csp6I GTAC 3 cut(s) 227, 415, 760
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 4 cut(s) 37, 220, 494, 691
CviQI GTAC 3 cut(s) 227, 415, 760
DdeI CTNAG 1 cut(s) 411
DpnI GATC 3 cut(s) 42, 746, 908
DpnII GATC 3 cut(s) 40, 744, 906
EaeI YGGCCR 1 cut(s) 693
Eam1104I CTCTTC 1 cut(s) 872
EarI CTCTTC 1 cut(s) 872
Eco130I CCWWGG 1 cut(s) 493
Eco147I AGGCCT 2 cut(s) 50, 700
Eco24I GRGCYC 1 cut(s) 385
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 245
Eco72I CACGTG 1 cut(s) 241
Eco88I CYCGRG 3 cut(s) 96, 672, 702
EcoO109I RGGNCCY 3 cut(s) 245, 381, 382
EcoRII CCWGG 3 cut(s) 678, 695, 737
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 493
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 4 cut(s) 40, 223, 497, 694
FaiI YATR 9 cut(s) 38, 68, 221, 366, 368, 495, 576, 692, 721
FalI AAGNNNNNCTT 4 cut(s) 314, 346, 531, 563
FaqI GGGAC 2 cut(s) 231, 505
FatI CATG 4 cut(s) 36, 219, 493, 690
FauI CCCGC 1 cut(s) 97
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 7 cut(s) 144, 233, 236, 375, 594, 626, 883
FokI GGATG 5 cut(s) 190, 485, 511, 526, 795
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 7 cut(s) 144, 233, 236, 375, 594, 626, 883
FspBI CTAG 1 cut(s) 728
GlaI GCGC 4 cut(s) 592, 629, 655, 773
GluI GCNGC 7 cut(s) 144, 233, 236, 375, 594, 626, 883
GsuI CTGGAG 1 cut(s) 809
HaeII RGCGCY 1 cut(s) 657
HaeIII GGCC 8 cut(s) 50, 383, 401, 678, 695, 700, 755, 885
HapII CCGG 1 cut(s) 56
HgaI GACGC 2 cut(s) 110, 164
HhaI GCGC 4 cut(s) 593, 630, 656, 774
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 4 cut(s) 40, 223, 497, 694
Hin6I GCGC 4 cut(s) 591, 628, 654, 772
HinP1I GCGC 4 cut(s) 591, 628, 654, 772
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 4 cut(s) 149, 440, 634, 663
HpaII CCGG 1 cut(s) 56
HphI GGTGA 2 cut(s) 204, 836
Hpy166II GTNNAC 1 cut(s) 869
Hpy188I TCNGA 3 cut(s) 154, 660, 911
Hpy188III TCNNGA 4 cut(s) 98, 556, 667, 728
Hpy8I GTNNAC 1 cut(s) 869
Hpy99I CGWCG 2 cut(s) 158, 782
HpyAV CCTTC 5 cut(s) 53, 185, 265, 812, 821
HpyCH4III ACNGT 2 cut(s) 76, 607
HpyCH4IV ACGT 2 cut(s) 110, 240
HpyCH4V TGCA 4 cut(s) 46, 66, 377, 566
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 91, 327, 380
HpyF3I CTNAG 1 cut(s) 411
HpySE526I ACGT 2 cut(s) 110, 240
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 4 cut(s) 40, 223, 497, 694
HspAI GCGC 4 cut(s) 591, 628, 654, 772
KflI GGGWCCC 1 cut(s) 245
Kzo9I GATC 3 cut(s) 40, 744, 906
LguI GCTCTTC 1 cut(s) 872
LmnI GCTCC 2 cut(s) 274, 622
Lsp1109I GCAGC 4 cut(s) 155, 361, 580, 637
LweI GCATC 1 cut(s) 504
MaeI CTAG 1 cut(s) 728
MaeII ACGT 2 cut(s) 110, 240
MaeIII GTNAC 2 cut(s) 210, 601
MalI GATC 3 cut(s) 42, 746, 908
MbiI CCGCTC 1 cut(s) 882
MboI GATC 3 cut(s) 40, 744, 906
MboII GAAGA 4 cut(s) 39, 280, 367, 889
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 3 cut(s) 110, 385, 588
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 3 cut(s) 12, 501, 615
MluNI TGGCCA 1 cut(s) 695
MlyI GAGTC 2 cut(s) 434, 672
MmeI TCCRAC 2 cut(s) 177, 819
Mox20I TGGCCA 1 cut(s) 695
MscI TGGCCA 1 cut(s) 695
MseI TTAA 1 cut(s) 614
Msp20I TGGCCA 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 1 cut(s) 56
MspR9I CCNGG 3 cut(s) 680, 697, 739
MvaI CCWGG 3 cut(s) 680, 697, 739
MvnI CGCG 2 cut(s) 104, 591
MwoI GCNNNNNNNGC 4 cut(s) 52, 91, 327, 380
NcoI CCATGG 1 cut(s) 493
NdeII GATC 3 cut(s) 40, 744, 906
NlaIII CATG 4 cut(s) 40, 223, 497, 694
NlaIV GGNNCC 4 cut(s) 173, 246, 247, 383
NmuCI GTSAC 2 cut(s) 210, 601
PaeR7I CTCGAG 1 cut(s) 96
PasI CCCWGGG 1 cut(s) 738
PceI AGGCCT 2 cut(s) 50, 700
PciSI GCTCTTC 1 cut(s) 872
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 399
PfeI GAWTC 2 cut(s) 149, 634
PflFI GACNNNGTC 1 cut(s) 158
PflMI CCANNNNNTGG 1 cut(s) 396
PkrI GCNGC 7 cut(s) 145, 234, 237, 376, 595, 627, 884
PleI GAGTC 2 cut(s) 434, 671
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
PpsI GAGTC 2 cut(s) 434, 671
Ppu21I YACGTR 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 3 cut(s) 678, 695, 737
PspCI CACGTG 1 cut(s) 241
PspGI CCWGG 3 cut(s) 678, 695, 737
PspN4I GGNNCC 4 cut(s) 173, 246, 247, 383
PspOMI GGGCCC 1 cut(s) 381
PspPI GGNCC 5 cut(s) 245, 381, 382, 399, 754
PspPPI RGGWCCY 1 cut(s) 245
PstI CTGCAG 1 cut(s) 48
PsuI RGATCY 1 cut(s) 40
PsyI GACNNNGTC 1 cut(s) 158
RsaI GTAC 3 cut(s) 228, 416, 761
RsaNI GTAC 3 cut(s) 227, 415, 760
SapI GCTCTTC 1 cut(s) 872
SaqAI TTAA 1 cut(s) 614
SatI GCNGC 7 cut(s) 144, 233, 236, 375, 594, 626, 883
Sau3AI GATC 3 cut(s) 40, 744, 906
Sau96I GGNCC 5 cut(s) 245, 381, 382, 399, 754
ScaI AGTACT 1 cut(s) 416
SchI GAGTC 2 cut(s) 434, 672
ScrFI CCNGG 3 cut(s) 680, 697, 739
SduI GDGCHC 3 cut(s) 110, 385, 588
SfaNI GCATC 1 cut(s) 504
SfcI CTRYAG 1 cut(s) 44
Sfr274I CTCGAG 1 cut(s) 96
SinI GGWCC 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 96
SmlI CTYRAG 2 cut(s) 96, 442
SmoI CTYRAG 2 cut(s) 96, 442
Sse9I AATT 3 cut(s) 12, 501, 615
SseBI AGGCCT 2 cut(s) 50, 700
SsiI CCGC 8 cut(s) 104, 146, 233, 236, 255, 589, 596, 882
SspMI CTAG 1 cut(s) 728
StuI AGGCCT 2 cut(s) 50, 700
StyD4I CCNGG 3 cut(s) 678, 695, 737
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 2 cut(s) 76, 607
TaiI ACGT 2 cut(s) 113, 243
TaqI TCGA 2 cut(s) 97, 312
TasI AATT 3 cut(s) 12, 501, 615
TatI WGTACW 1 cut(s) 414
TauI GCSGC 3 cut(s) 235, 238, 885
TfiI GAWTC 2 cut(s) 149, 634
Tru1I TTAA 1 cut(s) 614
Tru9I TTAA 1 cut(s) 614
TscAI CASTG 3 cut(s) 342, 612, 878
TseFI GTSAC 2 cut(s) 210, 601
TseI GCWGC 4 cut(s) 143, 374, 593, 625
Tsp45I GTSAC 2 cut(s) 210, 601
TspGWI ACGGA 3 cut(s) 214, 526, 795
TspRI CASTG 3 cut(s) 342, 612, 878
Tth111I GACNNNGTC 1 cut(s) 158
Van91I CCANNNNNTGG 1 cut(s) 396
VpaK11BI GGWCC 1 cut(s) 245
XbaI TCTAGA 1 cut(s) 727
XhoI CTCGAG 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 868
XspI CTAG 1 cut(s) 728
ZrmI AGTACT 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.