RLG00000025904

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000189
Physical Location & Seq
Reverse (-)
49093275 .. 49096023
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025904

Sequence Viewer

Length: 864 bp
ATGCAGCAGCCGCAGCTGGACTTGCAAGCTTTGCCGGAAGGTTGCATGGCCAACGTTATCTCATCGACAAGTCCTCCAGATGCATGCAGGCTCTCGTTGCTTTCTAAGGTTATCAGGTCGGCCGCTGAATCCGACGCCGTTTGGGACAAGTTCATTCCGCCTGAGACATACACGATCCTTTCCCAGTCCAATTCCCTTTCCTCTTCCCCTCCCAAATCCAAGAAGGAGCTCTACATGGCTCTCTGCGATGAACCCGTCCTCATCGACGATGGCAATATGAGCTTTTCACTGGACAAATGGAGTGGGAAGAAGTGCTATATGATAGCTGCAAAGCGCCTTCGGATTGCTTGGGGTGATACTCCTAATTATTGGACATGGAAATCTCTGCCTGAGTGCAGGTTTAAGGAGGCGGCCAAGCTTCGTTTTGTTTGCTGGCTTGAAATCCGTGGGAGAATTGAAACGCGCATGCTGTCCCCATCCACAACGTATAAAGCTTATCTGGTGTACAAGTTAACTAAACGCACTTATGGACTAGACTGCCCTGCCGTGGTCACAGTTGGTGCAAGAGCTTATGGATTTGATTATGAATGCCCTCGTTTGAAGGAAATAACTGCAGGCATTAAGCACACTGCGATTCTGGTGCCCCAAAGAAAAATAGAAGGTCATCTCGCAAGACATCATGAAATCAATGAAGTTAGATTTCCAAACGAAAGCAGGGCGGATGGGTGGCTGGAGATGGAGCTGGGTGAGTTTCACTGTCAAGGAGACGAAGACGGGGAGTTGGAAATGATTTGTTGTGAGACTAGTATGGGTAAGAGTGGTCTCCTTGTTCAAGGGATTGAGGTTAGACCAAAAGGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

288

Amino Acids

32.39

Weight (kDa)

6.94

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 103 - 284 6e-47 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 387
AccB1I GGYRCC 1 cut(s) 640
AccII CGCG 1 cut(s) 463
AciI CCGC 5 cut(s) 11, 123, 158, 410, 719
AclI AACGTT 1 cut(s) 54
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 3 cut(s) 48, 120, 411
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 547
AgsI TTSAA 4 cut(s) 440, 458, 601, 833
AhlI ACTAGT 1 cut(s) 803
AluBI AGCT 9 cut(s) 16, 29, 229, 282, 326, 418, 494, 569, 742
AluI AGCT 9 cut(s) 16, 29, 229, 282, 326, 418, 494, 569, 742
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 4 cut(s) 158, 759, 794, 827
AlwI GGATC 1 cut(s) 169
AoxI GGCC 3 cut(s) 48, 120, 411
ApeKI GCWGC 4 cut(s) 4, 7, 13, 326
AspLEI GCGC 2 cut(s) 336, 465
AsuHPI GGTGA 2 cut(s) 365, 758
BaeGI GKGCMC 1 cut(s) 645
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 1 cut(s) 640
BanII GRGCYC 1 cut(s) 231
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BbsI GAAGAC 1 cut(s) 777
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 16, 19, 25, 313
BccI CCATC 4 cut(s) 263, 484, 716, 730
BceAI ACGGC 2 cut(s) 122, 530
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BcoDI GTCTC 4 cut(s) 158, 759, 794, 827
BcuI ACTAGT 1 cut(s) 803
BfaI CTAG 3 cut(s) 533, 804, 862
BfmI CTRYAG 1 cut(s) 612
BfoI RGCGCY 1 cut(s) 337
BfuAI ACCTGC 1 cut(s) 387
BisI GCNGC 7 cut(s) 5, 8, 11, 14, 123, 327, 411
BlsI GCNGC 7 cut(s) 6, 9, 12, 15, 124, 328, 412
BmiI GGNNCC 1 cut(s) 642
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 777
BpmI CTGGAG 2 cut(s) 60, 752
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 827
BsaJI CCNNGG 2 cut(s) 445, 546
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 2 cut(s) 184, 294
BseDI CCNNGG 2 cut(s) 445, 546
BseGI GGATG 2 cut(s) 476, 727
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 2 cut(s) 153, 381
BseNI ACTGG 2 cut(s) 184, 294
BseSI GKGCMC 1 cut(s) 645
BseX3I CGGCCG 1 cut(s) 120
BseXI GCAGC 4 cut(s) 16, 19, 25, 313
BseYI CCCAGC 1 cut(s) 742
BsgI GTGCAG 1 cut(s) 415
Bsh1236I CGCG 1 cut(s) 463
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 3 cut(s) 50, 122, 413
BshNI GGYRCC 1 cut(s) 640
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 2 cut(s) 158, 457
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 4 cut(s) 158, 759, 794, 827
BsmBI CGTCTC 1 cut(s) 759
BsmFI GGGAC 2 cut(s) 158, 457
BsmI GAATGC 1 cut(s) 593
BsnI GGCC 3 cut(s) 50, 122, 413
Bso31I GGTCTC 1 cut(s) 827
Bsp1286I GDGCHC 2 cut(s) 231, 645
Bsp1407I TGTACA 1 cut(s) 504
Bsp143I GATC 1 cut(s) 174
BspACI CCGC 5 cut(s) 11, 123, 158, 410, 719
BspANI GGCC 3 cut(s) 50, 122, 413
BspCNI CTCAG 2 cut(s) 154, 382
BspFNI CGCG 1 cut(s) 463
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 1 cut(s) 642
BspMAI CTGCAG 1 cut(s) 616
BspMI ACCTGC 1 cut(s) 387
BspPI GGATC 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 640
BspTNI GGTCTC 1 cut(s) 827
BsrGI TGTACA 1 cut(s) 504
BsrI ACTGG 2 cut(s) 184, 294
BssECI CCNNGG 2 cut(s) 445, 546
BssMI GATC 1 cut(s) 174
BssNI GRCGYC 1 cut(s) 135
Bst4CI ACNGT 2 cut(s) 556, 758
Bst6I CTCTTC 1 cut(s) 208
BstACI GRCGYC 1 cut(s) 135
BstAPI GCANNNNNTGC 1 cut(s) 31
BstAUI TGTACA 1 cut(s) 504
BstC8I GCNNGC 6 cut(s) 27, 85, 89, 434, 467, 616
BstDEI CTNAG 3 cut(s) 105, 162, 390
BstDSI CCRYGG 2 cut(s) 445, 546
BstF5I GGATG 2 cut(s) 476, 727
BstFNI CGCG 1 cut(s) 463
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 2 cut(s) 336, 465
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 4 cut(s) 158, 759, 794, 827
BstMBI GATC 1 cut(s) 174
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 6 cut(s) 10, 13, 22, 31, 97, 279
BstNSI RCATGY 2 cut(s) 87, 469
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 645
BstUI CGCG 1 cut(s) 463
BstV1I GCAGC 4 cut(s) 16, 19, 25, 313
BstV2I GAAGAC 1 cut(s) 777
BstZI CGGCCG 1 cut(s) 120
BsuRI GGCC 3 cut(s) 50, 122, 413
BtgI CCRYGG 2 cut(s) 445, 546
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 476, 727
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 3 cut(s) 287, 627, 754
BveI ACCTGC 1 cut(s) 387
Cac8I GCNNGC 6 cut(s) 27, 85, 89, 434, 467, 616
CciI TCATGA 1 cut(s) 679
CfoI GCGC 2 cut(s) 336, 465
CseI GACGC 1 cut(s) 143
Csp6I GTAC 1 cut(s) 505
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 6 cut(s) 46, 84, 235, 375, 466, 680
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 3 cut(s) 105, 162, 390
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
EaeI YGGCCR 3 cut(s) 48, 120, 411
EagI CGGCCG 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
EciI GGCGGA 2 cut(s) 147, 734
Ecl136II GAGCTC 1 cut(s) 229
EclXI CGGCCG 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 827
Eco52I CGGCCG 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 759
FaeI CATG 6 cut(s) 49, 87, 238, 378, 469, 683
FaqI GGGAC 2 cut(s) 158, 457
FatI CATG 6 cut(s) 45, 83, 234, 374, 465, 679
Fnu4HI GCNGC 7 cut(s) 5, 8, 11, 14, 123, 327, 411
FokI GGATG 2 cut(s) 463, 734
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 7 cut(s) 5, 8, 11, 14, 123, 327, 411
FspBI CTAG 3 cut(s) 533, 804, 862
GlaI GCGC 2 cut(s) 335, 464
GluI GCNGC 7 cut(s) 5, 8, 11, 14, 123, 327, 411
GsaI CCCAGC 1 cut(s) 746
GsuI CTGGAG 2 cut(s) 60, 752
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 3 cut(s) 50, 122, 413
HapII CCGG 1 cut(s) 35
HgaI GACGC 1 cut(s) 143
HhaI GCGC 2 cut(s) 336, 465
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 6 cut(s) 49, 87, 238, 378, 469, 683
Hin6I GCGC 2 cut(s) 334, 463
HinP1I GCGC 2 cut(s) 334, 463
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HindIII AAGCTT 3 cut(s) 27, 416, 492
HinfI GANTC 2 cut(s) 128, 634
HpaI GTTAAC 1 cut(s) 513
HpaII CCGG 1 cut(s) 35
HphI GGTGA 2 cut(s) 365, 758
Hpy166II GTNNAC 2 cut(s) 505, 513
Hpy188I TCNGA 2 cut(s) 133, 342
Hpy188III TCNNGA 2 cut(s) 77, 680
Hpy8I GTNNAC 2 cut(s) 505, 513
Hpy99I CGWCG 2 cut(s) 137, 269
HpyAV CCTTC 5 cut(s) 32, 217, 347, 595, 653
HpyCH4III ACNGT 2 cut(s) 556, 758
HpyCH4IV ACGT 2 cut(s) 54, 485
HpyCH4V TGCA 9 cut(s) 4, 25, 45, 83, 87, 329, 396, 563, 614
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 13, 22, 31, 97, 279
HpyF3I CTNAG 3 cut(s) 105, 162, 390
HpySE526I ACGT 2 cut(s) 54, 485
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 6 cut(s) 49, 87, 238, 378, 469, 683
HspAI GCGC 2 cut(s) 334, 463
KspAI GTTAAC 1 cut(s) 513
Kzo9I GATC 1 cut(s) 174
LmnI GCTCC 2 cut(s) 226, 739
Lsp1109I GCAGC 4 cut(s) 16, 19, 25, 313
LweI GCATC 1 cut(s) 70
MaeI CTAG 3 cut(s) 533, 804, 862
MaeII ACGT 2 cut(s) 54, 485
MaeIII GTNAC 1 cut(s) 550
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 3 cut(s) 195, 319, 782
MhlI GDGCHC 2 cut(s) 231, 645
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 3 cut(s) 190, 364, 453
MluNI TGGCCA 1 cut(s) 50
MmeI TCCRAC 2 cut(s) 156, 762
MnlI CCTC 7 cut(s) 84, 211, 219, 269, 400, 603, 835
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 1 cut(s) 85
MscI TGGCCA 1 cut(s) 50
MseI TTAA 3 cut(s) 402, 512, 621
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 2 cut(s) 16, 125
MspI CCGG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 593
MvnI CGCG 1 cut(s) 463
MwoI GCNNNNNNNGC 6 cut(s) 10, 13, 22, 31, 97, 279
NdeII GATC 1 cut(s) 174
NlaIII CATG 6 cut(s) 49, 87, 238, 378, 469, 683
NlaIV GGNNCC 1 cut(s) 642
NmuCI GTSAC 1 cut(s) 550
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 2 cut(s) 87, 469
PaeI GCATGC 2 cut(s) 87, 469
PagI TCATGA 1 cut(s) 679
PctI GAATGC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 128, 634
PkrI GCNGC 7 cut(s) 6, 9, 12, 15, 124, 328, 412
Psp124BI GAGCTC 1 cut(s) 231
Psp1406I AACGTT 1 cut(s) 54
PspFI CCCAGC 1 cut(s) 742
PspN4I GGNNCC 1 cut(s) 642
PstI CTGCAG 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 3 cut(s) 402, 512, 621
SatI GCNGC 7 cut(s) 5, 8, 11, 14, 123, 327, 411
Sau3AI GATC 1 cut(s) 174
SduI GDGCHC 2 cut(s) 231, 645
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 612
SpeI ACTAGT 1 cut(s) 803
SphI GCATGC 2 cut(s) 87, 469
Sse9I AATT 3 cut(s) 190, 364, 453
SsiI CCGC 5 cut(s) 11, 123, 158, 410, 719
SspMI CTAG 3 cut(s) 533, 804, 862
SstI GAGCTC 1 cut(s) 231
TaaI ACNGT 2 cut(s) 556, 758
TaiI ACGT 2 cut(s) 57, 488
TaqI TCGA 2 cut(s) 65, 264
TasI AATT 3 cut(s) 190, 364, 453
TatI WGTACW 1 cut(s) 504
TauI GCSGC 3 cut(s) 13, 125, 413
TfiI GAWTC 2 cut(s) 128, 634
Tru1I TTAA 3 cut(s) 402, 512, 621
Tru9I TTAA 3 cut(s) 402, 512, 621
TscAI CASTG 3 cut(s) 294, 634, 761
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 4 cut(s) 4, 7, 13, 326
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 5 cut(s) 142, 264, 600, 696, 705
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 3 cut(s) 294, 634, 761
XceI RCATGY 2 cut(s) 87, 469
XspI CTAG 3 cut(s) 533, 804, 862
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.