MD12G1255600.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000189
Physical Location & Seq
Forward (+)
32352072 .. 32354560
2489 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1255600.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGATCTGCAGGCCTTGCCGGAAGGGTGCATAGCCACCGTTGTCTCGCTGACCACGCCTCGAGACGCAGGCACGTTGTCGTCGGTTTCGAGGAGTTTCAGGTCGGCAGCGGAATCCGACACCGTCTGGGACAGGTTCCTTCCACCCGATATCCACACGATCCTGTCCTCCTCGTCACCCTCCATGTCCGTACATCCGCCGCATGTGTCTCCCTCCTCCGCTTCCAAAACCAAGAAGGAGCTTTACCTCGCTCTATGCGACAACCCTGTTCTCATCGAACAGGGAAAGTTGAGCTTTTCACTGGACAGATGGAGCGGGAAGAAATGCTATATGATTGCTGCAAGGGCACTTTCGATCGTTTGGGGCGACACTCCTCAATACTGGGAATGGATTTCTCTCCCCGACTCGAGGTTTGAGGAGGTGGCAAAGCTTATGTGTGTGTGTTGGCTTGAAATCCGTGGCAAAATTGAGACACGGATGCTGTCCCCGGCCACCCTTTATAAAGCTTATCTTGTATTCAAGCCAACTACAGGGGCTTATGGATTTGACCAACAACCTGTGGAGGTCACAGTCGGTTTAATTGGGGAGGAGCCCACGAAAAACGAGGTGTTTTTGAACGCTCAGAGAGGCCTTGGTTCCCAAGGCCCCAGCTTTCCCTTGGCCGGGCCTCTGCGTATTAGCGTCTTCAACCGGAGATACATTGTGGGAGTCCAAGATTTTCAGCTCAGAGAGATCAAGGTCAATGAGGCCCAGTACCCGAAAGAGCGCAACGACGGGTGGCTGGAGATAGAGATGGGGGTGTTCTTCTGTGAGGGGGGTGAAGGTGGAGAGTTGGAGATGGCTTGCTTAGAGGTTAGGCGTATAAACTGGAAGGGTGGCCTCTTGTTTCAAGGGATTGAAATCAGGCCCAAAAGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0030246 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

307

Amino Acids

34.24

Weight (kDa)

6.04

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 107 - 303 1.8e-57 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AccBSI CCGCTC 1 cut(s) 315
AciI CCGC 5 cut(s) 110, 197, 200, 219, 315
AclWI GGATC 2 cut(s) 12, 154
AcoI YGGCCR 2 cut(s) 489, 660
AfaI GTAC 2 cut(s) 192, 755
AfiI CCNNNNNNNGG 4 cut(s) 408, 530, 662, 663
AgsI TTSAA 6 cut(s) 452, 520, 616, 688, 890, 899
AluBI AGCT 6 cut(s) 241, 294, 430, 506, 651, 724
AluI AGCT 6 cut(s) 241, 294, 430, 506, 651, 724
Alw26I GTCTC 4 cut(s) 49, 57, 213, 464
AlwI GGATC 2 cut(s) 12, 154
Ama87I CYCGRG 2 cut(s) 60, 406
AoxI GGCC 9 cut(s) 12, 489, 628, 643, 660, 665, 747, 877, 905
ApeKI GCWGC 2 cut(s) 107, 338
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
AspLEI GCGC 1 cut(s) 768
AspS9I GGNCC 4 cut(s) 644, 665, 748, 906
AsuC2I CCSGG 2 cut(s) 488, 664
AsuHPI GGTGA 2 cut(s) 168, 830
AvaI CYCGRG 2 cut(s) 60, 406
BaeGI GKGCMC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 594
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BbsI GAAGAC 1 cut(s) 676
BbvI GCAGC 2 cut(s) 119, 325
BccI CCATC 3 cut(s) 303, 787, 832
BcnI CCSGG 2 cut(s) 488, 664
BcoDI GTCTC 4 cut(s) 49, 57, 213, 464
BfmI CTRYAG 2 cut(s) 8, 528
BisI GCNGC 3 cut(s) 108, 200, 339
BlsI GCNGC 3 cut(s) 109, 201, 340
Bme1390I CCNGG 2 cut(s) 488, 664
BmeT110I CYCGRG 2 cut(s) 60, 406
BmgT120I GGNCC 4 cut(s) 644, 665, 748, 906
BmiI GGNNCC 4 cut(s) 137, 591, 637, 646
BmrFI CCNGG 2 cut(s) 488, 664
BmrI ACTGGG 2 cut(s) 391, 745
BmsI GCATC 1 cut(s) 468
BmuI ACTGGG 2 cut(s) 391, 745
BpiI GAAGAC 1 cut(s) 676
BpmI CTGGAG 1 cut(s) 803
BpuMI CCSGG 2 cut(s) 488, 664
BsaBI GATNNNNATC 1 cut(s) 899
BsaJI CCNNGG 5 cut(s) 457, 486, 631, 640, 657
BsaWI WCCGGW 1 cut(s) 690
Bsc4I CCNNNNNNNGG 4 cut(s) 408, 530, 662, 663
Bse1I ACTGG 4 cut(s) 306, 386, 751, 872
Bse8I GATNNNNATC 1 cut(s) 899
BseDI CCNNGG 5 cut(s) 457, 486, 631, 640, 657
BseGI GGATG 3 cut(s) 193, 483, 921
BseJI GATNNNNATC 1 cut(s) 899
BseLI CCNNNNNNNGG 4 cut(s) 408, 530, 662, 663
BseMII CTCAG 2 cut(s) 635, 739
BseNI ACTGG 4 cut(s) 306, 386, 751, 872
BseRI GAGGAG 6 cut(s) 106, 160, 205, 363, 431, 602
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 2 cut(s) 119, 325
BseYI CCCAGC 1 cut(s) 647
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 9 cut(s) 14, 491, 630, 645, 662, 667, 749, 879, 907
BsiEI CGRYCG 1 cut(s) 357
BsiHKCI CYCGRG 2 cut(s) 60, 406
BsiSI CCGG 4 cut(s) 20, 488, 663, 691
BslFI GGGAC 2 cut(s) 143, 469
BslI CCNNNNNNNGG 4 cut(s) 408, 530, 662, 663
BsmAI GTCTC 4 cut(s) 49, 57, 213, 464
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 143, 469
BsnI GGCC 9 cut(s) 14, 491, 630, 645, 662, 667, 749, 879, 907
BsoBI CYCGRG 2 cut(s) 60, 406
Bsp1286I GDGCHC 2 cut(s) 349, 594
Bsp143I GATC 4 cut(s) 4, 159, 354, 732
BspACI CCGC 5 cut(s) 110, 197, 200, 219, 315
BspANI GGCC 9 cut(s) 14, 491, 630, 645, 662, 667, 749, 879, 907
BspCNI CTCAG 2 cut(s) 634, 738
BspLI GGNNCC 4 cut(s) 137, 591, 637, 646
BspMAI CTGCAG 1 cut(s) 12
BspPI GGATC 2 cut(s) 12, 154
BsrBI CCGCTC 1 cut(s) 315
BsrI ACTGG 4 cut(s) 306, 386, 751, 872
BssECI CCNNGG 5 cut(s) 457, 486, 631, 640, 657
BssMI GATC 4 cut(s) 4, 159, 354, 732
BssT1I CCWWGG 3 cut(s) 631, 640, 657
Bst4CI ACNGT 3 cut(s) 40, 124, 571
BstAPI GCANNNNNTGC 1 cut(s) 16
BstC8I GCNNGC 3 cut(s) 12, 70, 844
BstDEI CTNAG 3 cut(s) 621, 725, 847
BstDSI CCRYGG 1 cut(s) 457
BstF5I GGATG 3 cut(s) 193, 483, 921
BstHHI GCGC 1 cut(s) 768
BstKTI GATC 4 cut(s) 7, 162, 357, 735
BstMAI GTCTC 4 cut(s) 49, 57, 213, 464
BstMBI GATC 4 cut(s) 4, 159, 354, 732
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 3 cut(s) 16, 55, 344
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 486, 662
BstSFI CTRYAG 2 cut(s) 8, 528
BstSLI GKGCMC 1 cut(s) 349
BstV1I GCAGC 2 cut(s) 119, 325
BstV2I GAAGAC 1 cut(s) 676
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 9 cut(s) 14, 491, 630, 645, 662, 667, 749, 879, 907
BtgI CCRYGG 1 cut(s) 457
BtsCI GGATG 3 cut(s) 193, 483, 921
BtsIMutI CAGTG 1 cut(s) 299
Cac8I GCNNGC 3 cut(s) 12, 70, 844
CfoI GCGC 1 cut(s) 768
Cfr13I GGNCC 4 cut(s) 644, 665, 748, 906
CseI GACGC 2 cut(s) 74, 670
Csp6I GTAC 2 cut(s) 191, 754
CviAII CATG 2 cut(s) 184, 203
CviQI GTAC 2 cut(s) 191, 754
DdeI CTNAG 3 cut(s) 621, 725, 847
DpnI GATC 4 cut(s) 6, 161, 356, 734
DpnII GATC 4 cut(s) 4, 159, 354, 732
EaeI YGGCCR 2 cut(s) 489, 660
EciI GGCGGA 1 cut(s) 186
Eco130I CCWWGG 3 cut(s) 631, 640, 657
Eco147I AGGCCT 2 cut(s) 14, 630
Eco24I GRGCYC 1 cut(s) 594
Eco32I GATATC 1 cut(s) 151
Eco88I CYCGRG 2 cut(s) 60, 406
EcoO109I RGGNCCY 1 cut(s) 644
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 3 cut(s) 631, 640, 657
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 3 cut(s) 631, 640, 657
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 2 cut(s) 187, 206
FalI AAGNNNNNCTT 4 cut(s) 278, 310, 495, 527
FaqI GGGAC 2 cut(s) 143, 469
FatI CATG 2 cut(s) 183, 202
FauI CCCGC 1 cut(s) 308
Fnu4HI GCNGC 3 cut(s) 108, 200, 339
FokI GGATG 2 cut(s) 180, 490
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 3 cut(s) 108, 200, 339
GlaI GCGC 1 cut(s) 767
GluI GCNGC 3 cut(s) 108, 200, 339
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 1 cut(s) 803
HaeIII GGCC 9 cut(s) 14, 491, 630, 645, 662, 667, 749, 879, 907
HapII CCGG 4 cut(s) 20, 488, 663, 691
HgaI GACGC 2 cut(s) 74, 670
HhaI GCGC 1 cut(s) 768
Hin1II CATG 2 cut(s) 187, 206
Hin6I GCGC 1 cut(s) 766
HinP1I GCGC 1 cut(s) 766
HindIII AAGCTT 2 cut(s) 428, 504
HinfI GANTC 3 cut(s) 113, 404, 708
HpaII CCGG 4 cut(s) 20, 488, 663, 691
HphI GGTGA 2 cut(s) 168, 830
Hpy188I TCNGA 3 cut(s) 118, 624, 728
Hpy188III TCNNGA 1 cut(s) 62
Hpy99I CGWCG 2 cut(s) 85, 776
HpyAV CCTTC 5 cut(s) 17, 149, 229, 815, 865
HpyCH4III ACNGT 3 cut(s) 40, 124, 571
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 3 cut(s) 10, 30, 341
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 55, 344
HpyF3I CTNAG 3 cut(s) 621, 725, 847
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 2 cut(s) 187, 206
HspAI GCGC 1 cut(s) 766
Kzo9I GATC 4 cut(s) 4, 159, 354, 732
LmnI GCTCC 3 cut(s) 238, 312, 589
Lsp1109I GCAGC 2 cut(s) 119, 325
LweI GCATC 1 cut(s) 468
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 2 cut(s) 174, 565
MalI GATC 4 cut(s) 6, 161, 356, 734
MbiI CCGCTC 1 cut(s) 315
MboI GATC 4 cut(s) 4, 159, 354, 732
MboII GAAGA 3 cut(s) 331, 676, 796
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 349, 594
MluCI AATT 2 cut(s) 465, 579
MlyI GAGTC 2 cut(s) 398, 717
MmeI TCCRAC 2 cut(s) 141, 813
MseI TTAA 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 4 cut(s) 20, 488, 663, 691
MspR9I CCNGG 2 cut(s) 488, 664
MwoI GCNNNNNNNGC 3 cut(s) 16, 55, 344
NciI CCSGG 2 cut(s) 488, 664
NdeII GATC 4 cut(s) 4, 159, 354, 732
NlaIII CATG 2 cut(s) 187, 206
NlaIV GGNNCC 4 cut(s) 137, 591, 637, 646
NmuCI GTSAC 2 cut(s) 174, 565
NspI RCATGY 1 cut(s) 206
PaeR7I CTCGAG 2 cut(s) 60, 406
PceI AGGCCT 2 cut(s) 14, 630
PcsI WCGNNNNNNNCGW 3 cut(s) 86, 255, 363
PfeI GAWTC 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 3 cut(s) 109, 201, 340
Ple19I CGATCG 1 cut(s) 357
PleI GAGTC 2 cut(s) 398, 716
PpsI GAGTC 2 cut(s) 398, 716
PsiI TTATAA 1 cut(s) 501
PspFI CCCAGC 1 cut(s) 647
PspN4I GGNNCC 4 cut(s) 137, 591, 637, 646
PspPI GGNCC 4 cut(s) 644, 665, 748, 906
PspXI VCTCGAGB 1 cut(s) 406
PstI CTGCAG 1 cut(s) 12
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 122
PvuI CGATCG 1 cut(s) 357
RsaI GTAC 2 cut(s) 192, 755
RsaNI GTAC 2 cut(s) 191, 754
SaqAI TTAA 1 cut(s) 578
SatI GCNGC 3 cut(s) 108, 200, 339
Sau3AI GATC 4 cut(s) 4, 159, 354, 732
Sau96I GGNCC 4 cut(s) 644, 665, 748, 906
SchI GAGTC 2 cut(s) 398, 717
ScrFI CCNGG 2 cut(s) 488, 664
SduI GDGCHC 2 cut(s) 349, 594
SfaNI GCATC 1 cut(s) 468
SfcI CTRYAG 2 cut(s) 8, 528
Sfr274I CTCGAG 2 cut(s) 60, 406
SlaI CTCGAG 2 cut(s) 60, 406
SmlI CTYRAG 2 cut(s) 60, 406
SmoI CTYRAG 2 cut(s) 60, 406
Sse9I AATT 2 cut(s) 465, 579
SseBI AGGCCT 2 cut(s) 14, 630
SsiI CCGC 5 cut(s) 110, 197, 200, 219, 315
StuI AGGCCT 2 cut(s) 14, 630
StyD4I CCNGG 2 cut(s) 486, 662
StyI CCWWGG 3 cut(s) 631, 640, 657
TaaI ACNGT 3 cut(s) 40, 124, 571
TaiI ACGT 1 cut(s) 77
TaqI TCGA 5 cut(s) 61, 89, 276, 353, 407
TasI AATT 2 cut(s) 465, 579
TauI GCSGC 1 cut(s) 202
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 578
Tru9I TTAA 1 cut(s) 578
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 2 cut(s) 174, 565
TseI GCWGC 2 cut(s) 107, 338
Tsp45I GTSAC 2 cut(s) 174, 565
TspGWI ACGGA 3 cut(s) 178, 446, 490
TspRI CASTG 1 cut(s) 306
Tth111I GACNNNGTC 1 cut(s) 122
XceI RCATGY 1 cut(s) 206
XcmI CCANNNNNNNNNTGG 1 cut(s) 655
XhoI CTCGAG 2 cut(s) 60, 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.