RchiOBHm_Chr2g0090041

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000189
Physical Location & Seq
Reverse (-)
4054792 .. 4055742
951 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46532

Sequence Viewer

Length: 951 bp
ATGAAGAAGAGAAACAGAGTGACGGAGAAGGTGAATAAGAACTCTGAGGAGGAGGAGGAATCACCTGCGACGGAGATGCAGAAGCAGCCGCTGCTGGACTTGCAAGCTTTGCCGGAAGGATGCATAGCCCACGTGATCTCGTTGACAAGTCCTCCGGATGCATGCAGGCTCTCCTTGCTTTCTAAGGTTATCAGGTCGGCCGCTGAATCCGACGCGGTTTGGGACAAGTTTATTCCGCATGATACACACGAGATTCTTTCCCAGTTCAATTCCCTTTCTTCTTCTTCCCCTCCAAAATCCAAGAAGGAGCTCTACATGGCTCTGTGCGACAACCCCGTCCTCATTGACGAGGGCTATACGAGCTTTTCACTGGAGAAATGGAGTGGGAAGAAGTGCTATATGATAGCTGCAAAGCGCCTTCGGATTGCTTGGGGTGATACTCCTCGATATTGGACATGGAAATCTGTGACTGAGTCCAGGTTTAAGGAGGCGGCCGAGCTTTCTGGTCAAGTATTTTGGCTTGAAATCCGTGGGAGAATTGAAAAGCGCATGCTGTCTCCATCCACGACGTATAAAGCTTATCTGGTGTACAAGTTAACTCAACACACTTATGGACTCAATTGGGCTGCCGTGGTCACAGTTTGTGCAAGAGCTTATGGCTTTGATTATGAATGCCCTGGTTTGAAGGAAATTACTGCAGGCATTAAGAGCACTGCGTTTCTGGCACCCCAAAGAGAAATAGATGGACAGCTCGCAAGAAATAAAAAAATAAAAAAAGCTAAATTTCCAAATGAAGGCAGGGCGGATGGGTGGCTGGAGATGGAGCTGGGTGAGTTTCACTATCAAGGGGACGAAGACGGGGAGTTGGAAATGATTTGTTGTGAGATCAGAGGTTCAAGTAAGAGTGGTCTCCTTGTTCAAGGGATTGAGGTCAGACCAAAAAGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

316

Amino Acids

35.88

Weight (kDa)

8.49

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 130 - 313 5.3e-44 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
AasI GACNNNNNNGTC 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 724
AccII CGCG 1 cut(s) 215
AccIII TCCGGA 1 cut(s) 154
AciI CCGC 6 cut(s) 89, 201, 215, 236, 491, 803
AcoI YGGCCR 2 cut(s) 198, 492
AcsI RAATTY 1 cut(s) 782
AcvI CACGTG 1 cut(s) 133
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 1 cut(s) 794
AgsI TTSAA 6 cut(s) 268, 524, 542, 685, 897, 920
AjnI CCWGG 2 cut(s) 476, 676
Alw21I GWGCWC 2 cut(s) 312, 713
Alw26I GTCTC 2 cut(s) 561, 914
AlwNI CAGNNNCTG 1 cut(s) 91
Aor13HI TCCGGA 1 cut(s) 154
AoxI GGCC 2 cut(s) 198, 492
ApeKI GCWGC 4 cut(s) 85, 91, 407, 626
ApoI RAATTY 1 cut(s) 782
AspLEI GCGC 2 cut(s) 417, 549
AsuHPI GGTGA 4 cut(s) 43, 54, 446, 842
BanI GGYRCC 1 cut(s) 724
BanII GRGCYC 1 cut(s) 312
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BauI CACGAG 1 cut(s) 248
BbrPI CACGTG 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 2 cut(s) 312, 713
BbvI GCAGC 4 cut(s) 78, 97, 394, 613
BccI CCATC 4 cut(s) 568, 737, 800, 814
BceAI ACGGC 1 cut(s) 614
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 2 cut(s) 478, 678
BcoDI GTCTC 2 cut(s) 561, 914
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 1 cut(s) 418
BfuAI ACCTGC 1 cut(s) 73
BisI GCNGC 7 cut(s) 86, 89, 92, 201, 408, 492, 627
BlsI GCNGC 7 cut(s) 87, 90, 93, 202, 409, 493, 628
Bme1390I CCNGG 2 cut(s) 478, 678
BmiI GGNNCC 1 cut(s) 726
BmrFI CCNGG 2 cut(s) 478, 678
BmrI ACTGGG 1 cut(s) 256
BmsI GCATC 3 cut(s) 66, 110, 148
BmuI ACTGGG 1 cut(s) 256
BpiI GAAGAC 1 cut(s) 861
BpmI CTGGAG 2 cut(s) 392, 836
BsaAI YACGTR 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 3 cut(s) 529, 630, 676
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 1 cut(s) 794
Bse1I ACTGG 2 cut(s) 262, 375
BseAI TCCGGA 1 cut(s) 154
BseBI CCWGG 2 cut(s) 478, 678
BseDI CCNNGG 3 cut(s) 529, 630, 676
BseGI GGATG 4 cut(s) 125, 163, 560, 811
BseLI CCNNNNNNNGG 1 cut(s) 794
BseMII CTCAG 2 cut(s) 36, 462
BseNI ACTGG 2 cut(s) 262, 375
BseRI GAGGAG 4 cut(s) 62, 65, 68, 432
BseX3I CGGCCG 2 cut(s) 198, 492
BseXI GCAGC 4 cut(s) 78, 97, 394, 613
BseYI CCCAGC 1 cut(s) 826
Bsh1236I CGCG 1 cut(s) 215
Bsh1285I CGRYCG 2 cut(s) 201, 495
BshFI GGCC 2 cut(s) 200, 494
BshNI GGYRCC 1 cut(s) 724
BsiEI CGRYCG 2 cut(s) 201, 495
BsiHKAI GWGCWC 2 cut(s) 312, 713
BsiSI CCGG 2 cut(s) 113, 155
BslFI GGGAC 2 cut(s) 236, 863
BslI CCNNNNNNNGG 1 cut(s) 794
BsmAI GTCTC 2 cut(s) 561, 914
BsmFI GGGAC 2 cut(s) 236, 863
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 2 cut(s) 200, 494
Bso31I GGTCTC 1 cut(s) 914
Bsp1286I GDGCHC 2 cut(s) 312, 713
Bsp13I TCCGGA 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 2 cut(s) 135, 885
BspACI CCGC 6 cut(s) 89, 201, 215, 236, 491, 803
BspANI GGCC 2 cut(s) 200, 494
BspCNI CTCAG 2 cut(s) 37, 463
BspEI TCCGGA 1 cut(s) 154
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 726
BspMAI CTGCAG 1 cut(s) 700
BspMI ACCTGC 1 cut(s) 73
BspT107I GGYRCC 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 914
BsrGI TGTACA 1 cut(s) 588
BsrI ACTGG 2 cut(s) 262, 375
BssECI CCNNGG 3 cut(s) 529, 630, 676
BssMI GATC 2 cut(s) 135, 885
BssSI CACGAG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 478, 678
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 1 cut(s) 2
BstAPI GCANNNNNTGC 2 cut(s) 91, 109
BstAUI TGTACA 1 cut(s) 588
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 6 cut(s) 105, 163, 167, 551, 700, 753
BstDEI CTNAG 3 cut(s) 45, 183, 471
BstDSI CCRYGG 2 cut(s) 529, 630
BstF5I GGATG 4 cut(s) 125, 163, 560, 811
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 2 cut(s) 417, 549
BstKTI GATC 2 cut(s) 138, 888
BstMAI GTCTC 2 cut(s) 561, 914
BstMBI GATC 2 cut(s) 135, 885
BstMCI CGRYCG 2 cut(s) 201, 495
BstMWI GCNNNNNNNGC 8 cut(s) 85, 91, 100, 109, 175, 360, 708, 722
BstNI CCWGG 2 cut(s) 478, 678
BstNSI RCATGY 2 cut(s) 165, 553
BstSCI CCNGG 2 cut(s) 476, 676
BstSFI CTRYAG 1 cut(s) 696
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 78, 97, 394, 613
BstV2I GAAGAC 1 cut(s) 861
BstZI CGGCCG 2 cut(s) 198, 492
BsuRI GGCC 2 cut(s) 200, 494
BtgI CCRYGG 2 cut(s) 529, 630
BtsCI GGATG 4 cut(s) 125, 163, 560, 811
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 2 cut(s) 368, 711
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 6 cut(s) 105, 163, 167, 551, 700, 753
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 2 cut(s) 417, 549
CseI GACGC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 589
CviAII CATG 5 cut(s) 162, 239, 316, 456, 550
CviQI GTAC 1 cut(s) 589
DdeI CTNAG 3 cut(s) 45, 183, 471
DpnI GATC 2 cut(s) 137, 887
DpnII GATC 2 cut(s) 135, 885
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 2 cut(s) 198, 492
EagI CGGCCG 2 cut(s) 198, 492
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
EciI GGCGGA 1 cut(s) 818
Ecl136II GAGCTC 1 cut(s) 310
EclXI CGGCCG 2 cut(s) 198, 492
Eco24I GRGCYC 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 914
Eco52I CGGCCG 2 cut(s) 198, 492
Eco53kI GAGCTC 1 cut(s) 310
Eco72I CACGTG 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 476, 676
EcoT22I ATGCAT 2 cut(s) 125, 163
EcoT38I GRGCYC 1 cut(s) 312
FaeI CATG 5 cut(s) 165, 242, 319, 459, 553
FaqI GGGAC 2 cut(s) 236, 863
FatI CATG 5 cut(s) 161, 238, 315, 455, 549
Fnu4HI GCNGC 7 cut(s) 86, 89, 92, 201, 408, 492, 627
FokI GGATG 4 cut(s) 132, 170, 547, 818
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 7 cut(s) 86, 89, 92, 201, 408, 492, 627
GlaI GCGC 2 cut(s) 416, 548
GluI GCNGC 7 cut(s) 86, 89, 92, 201, 408, 492, 627
GsaI CCCAGC 1 cut(s) 830
GsuI CTGGAG 2 cut(s) 392, 836
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 2 cut(s) 200, 494
HapII CCGG 2 cut(s) 113, 155
HgaI GACGC 1 cut(s) 221
HhaI GCGC 2 cut(s) 417, 549
Hin1II CATG 5 cut(s) 165, 242, 319, 459, 553
Hin6I GCGC 2 cut(s) 415, 547
HinP1I GCGC 2 cut(s) 415, 547
HincII GTYRAC 2 cut(s) 144, 597
HindII GTYRAC 2 cut(s) 144, 597
HindIII AAGCTT 2 cut(s) 105, 576
HinfI GANTC 5 cut(s) 59, 206, 253, 473, 615
HpaI GTTAAC 1 cut(s) 597
HpaII CCGG 2 cut(s) 113, 155
HphI GGTGA 4 cut(s) 43, 54, 446, 842
Hpy166II GTNNAC 3 cut(s) 144, 589, 597
Hpy188I TCNGA 5 cut(s) 46, 211, 423, 890, 935
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 3 cut(s) 144, 589, 597
Hpy99I CGWCG 3 cut(s) 73, 215, 571
HpyAV CCTTC 6 cut(s) 22, 110, 298, 428, 679, 788
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 2 cut(s) 132, 569
HpyCH4V TGCA 8 cut(s) 79, 103, 123, 161, 165, 410, 647, 698
HpyF10VI GCNNNNNNNGC 8 cut(s) 85, 91, 100, 109, 175, 360, 708, 722
HpyF3I CTNAG 3 cut(s) 45, 183, 471
HpySE526I ACGT 2 cut(s) 132, 569
Hsp92II CATG 5 cut(s) 165, 242, 319, 459, 553
HspAI GCGC 2 cut(s) 415, 547
Kpn2I TCCGGA 1 cut(s) 154
KspAI GTTAAC 1 cut(s) 597
Kzo9I GATC 2 cut(s) 135, 885
LmnI GCTCC 2 cut(s) 307, 823
Lsp1109I GCAGC 4 cut(s) 78, 97, 394, 613
LweI GCATC 3 cut(s) 66, 110, 148
MaeII ACGT 2 cut(s) 132, 569
MaeIII GTNAC 3 cut(s) 19, 466, 634
MalI GATC 2 cut(s) 137, 887
MboI GATC 2 cut(s) 135, 885
MboII GAAGA 7 cut(s) 16, 19, 270, 273, 276, 400, 866
MfeI CAATTG 1 cut(s) 619
MhlI GDGCHC 2 cut(s) 312, 713
MluCI AATT 5 cut(s) 268, 537, 619, 690, 782
MlyI GAGTC 2 cut(s) 482, 609
MmeI TCCRAC 2 cut(s) 234, 846
Mph1103I ATGCAT 2 cut(s) 125, 163
MroI TCCGGA 1 cut(s) 154
MseI TTAA 3 cut(s) 483, 596, 705
MslI CAYNNNNRTG 1 cut(s) 609
MspA1I CMGCKG 2 cut(s) 91, 203
MspI CCGG 2 cut(s) 113, 155
MspR9I CCNGG 2 cut(s) 478, 678
MunI CAATTG 1 cut(s) 619
Mva1269I GAATGC 1 cut(s) 677
MvaI CCWGG 2 cut(s) 478, 678
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 8 cut(s) 85, 91, 100, 109, 175, 360, 708, 722
NdeII GATC 2 cut(s) 135, 885
NlaIII CATG 5 cut(s) 165, 242, 319, 459, 553
NlaIV GGNNCC 1 cut(s) 726
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 3 cut(s) 19, 466, 634
NsiI ATGCAT 2 cut(s) 125, 163
NspI RCATGY 2 cut(s) 165, 553
PaeI GCATGC 2 cut(s) 165, 553
PaqCI CACCTGC 1 cut(s) 73
PctI GAATGC 1 cut(s) 677
PfeI GAWTC 3 cut(s) 59, 206, 253
PflFI GACNNNGTC 1 cut(s) 472
PkrI GCNGC 7 cut(s) 87, 90, 93, 202, 409, 493, 628
PleI GAGTC 2 cut(s) 481, 609
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 2 cut(s) 481, 609
Ppu21I YACGTR 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 476, 676
PspCI CACGTG 1 cut(s) 133
PspFI CCCAGC 1 cut(s) 826
PspGI CCWGG 2 cut(s) 476, 676
PspN4I GGNNCC 1 cut(s) 726
PstI CTGCAG 1 cut(s) 700
PstNI CAGNNNCTG 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 472
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
RseI CAYNNNNRTG 1 cut(s) 609
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 3 cut(s) 483, 596, 705
SatI GCNGC 7 cut(s) 86, 89, 92, 201, 408, 492, 627
Sau3AI GATC 2 cut(s) 135, 885
SchI GAGTC 2 cut(s) 482, 609
ScrFI CCNGG 2 cut(s) 478, 678
SduI GDGCHC 2 cut(s) 312, 713
SfaNI GCATC 3 cut(s) 66, 110, 148
SfcI CTRYAG 1 cut(s) 696
SmiMI CAYNNNNRTG 1 cut(s) 609
SphI GCATGC 2 cut(s) 165, 553
Sse9I AATT 5 cut(s) 268, 537, 619, 690, 782
SsiI CCGC 6 cut(s) 89, 201, 215, 236, 491, 803
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 2 cut(s) 476, 676
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 2 cut(s) 135, 572
TaqI TCGA 1 cut(s) 445
TasI AATT 5 cut(s) 268, 537, 619, 690, 782
TatI WGTACW 1 cut(s) 588
TauI GCSGC 3 cut(s) 91, 203, 494
TfiI GAWTC 3 cut(s) 59, 206, 253
Tru1I TTAA 3 cut(s) 483, 596, 705
Tru9I TTAA 3 cut(s) 483, 596, 705
TscAI CASTG 2 cut(s) 375, 718
TseFI GTSAC 3 cut(s) 19, 466, 634
TseI GCWGC 4 cut(s) 85, 91, 407, 626
Tsp45I GTSAC 3 cut(s) 19, 466, 634
TspDTI ATGAA 3 cut(s) 17, 684, 807
TspGWI ACGGA 3 cut(s) 38, 86, 518
TspRI CASTG 2 cut(s) 375, 718
Tth111I GACNNNGTC 1 cut(s) 472
XapI RAATTY 1 cut(s) 782
XceI RCATGY 2 cut(s) 165, 553
Zsp2I ATGCAT 2 cut(s) 125, 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.