MD04G1237300.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000189
Physical Location & Seq
Reverse (-)
31565293 .. 31565867
575 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1237300.v1.1.491

Sequence Viewer

Length: 330 bp
ATGGATCTGGTGGCGTTGCCAGAAGGATGCAAAGCCACCGTGGCCTCCTTAACGACGCCTCCTGACGCGTGCAGGCTTTCAGCGGTGTCTAGGGGTTTCCGGTCGGCCGCTGATTTGGACGCCGTTTGGGACAGGTTCCTTCCGCCTGATGTCTACACGATCCTCGGCAAGTGCTCCAGCTTTTCAGTGGACAAATGGAGTGGGAAAAAGGGCTATATGATATCTGCAAGGAAGCTTAAGATTACTTGGGGCGATACTCCTCAATATTGGGAATGGATTCGTCTGCCTGAATCCAGGTCTGTTAATAATTTTCATCCATATTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0030246 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

110

Amino Acids

12.24

Weight (kDa)

8.86

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 69 - 100 7.1e-10 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 153
AccII CGCG 1 cut(s) 68
AciI CCGC 3 cut(s) 83, 108, 143
AclWI GGATC 2 cut(s) 12, 154
AcoI YGGCCR 1 cut(s) 105
AcyI GRCGYC 2 cut(s) 56, 120
AflII CTTAAG 1 cut(s) 236
AflIII ACRYGT 1 cut(s) 66
AjnI CCWGG 1 cut(s) 293
AluBI AGCT 2 cut(s) 180, 235
AluI AGCT 2 cut(s) 180, 235
Alw21I GWGCWC 1 cut(s) 176
AlwI GGATC 2 cut(s) 12, 154
AoxI GGCC 2 cut(s) 42, 105
Asp700I GAANNNNTTC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 176
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 1 cut(s) 295
BfaI CTAG 1 cut(s) 90
BfrI CTTAAG 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 295
BmsI GCATC 1 cut(s) 17
BpmI CTGGAG 1 cut(s) 160
BsaHI GRCGYC 2 cut(s) 56, 120
BsaJI CCNNGG 2 cut(s) 39, 163
BsaWI WCCGGW 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
BseBI CCWGG 1 cut(s) 295
BseDI CCNNGG 2 cut(s) 39, 163
BseGI GGATG 2 cut(s) 32, 313
BseRI GAGGAG 1 cut(s) 249
BseX3I CGGCCG 1 cut(s) 105
BsgI GTGCAG 1 cut(s) 91
Bsh1236I CGCG 1 cut(s) 68
Bsh1285I CGRYCG 2 cut(s) 104, 108
BshFI GGCC 2 cut(s) 44, 107
BsiEI CGRYCG 2 cut(s) 104, 108
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 1 cut(s) 100
BslFI GGGAC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 2 cut(s) 44, 107
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 2 cut(s) 4, 159
BspACI CCGC 3 cut(s) 83, 108, 143
BspANI GGCC 2 cut(s) 44, 107
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 137
BspPI GGATC 2 cut(s) 12, 154
BspTI CTTAAG 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 39, 163
BssMI GATC 2 cut(s) 4, 159
BssNI GRCGYC 2 cut(s) 56, 120
Bst2UI CCWGG 1 cut(s) 295
Bst4CI ACNGT 1 cut(s) 40
BstACI GRCGYC 2 cut(s) 56, 120
BstAFI CTTAAG 1 cut(s) 236
BstC8I GCNNGC 2 cut(s) 70, 74
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 2 cut(s) 32, 313
BstFNI CGCG 1 cut(s) 68
BstKTI GATC 2 cut(s) 7, 162
BstMBI GATC 2 cut(s) 4, 159
BstMCI CGRYCG 2 cut(s) 104, 108
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstNI CCWGG 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 293
BstUI CGCG 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 2 cut(s) 44, 107
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 2 cut(s) 32, 313
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 70, 74
CseI GACGC 3 cut(s) 64, 74, 128
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviJI RGCY 7 cut(s) 35, 44, 76, 107, 180, 213, 235
CviKI_1 RGCY 7 cut(s) 35, 44, 76, 107, 180, 213, 235
DpnI GATC 2 cut(s) 6, 161
DpnII GATC 2 cut(s) 4, 159
EaeI YGGCCR 1 cut(s) 105
EagI CGGCCG 1 cut(s) 105
EciI GGCGGA 1 cut(s) 132
EclXI CGGCCG 1 cut(s) 105
Eco32I GATATC 1 cut(s) 222
Eco52I CGGCCG 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 293
EcoRV GATATC 1 cut(s) 222
FaiI YATR 3 cut(s) 216, 218, 319
FaqI GGGAC 1 cut(s) 143
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 2 cut(s) 39, 300
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 90
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 160
HaeIII GGCC 2 cut(s) 44, 107
HapII CCGG 1 cut(s) 100
HgaI GACGC 3 cut(s) 64, 74, 128
Hin1I GRCGYC 2 cut(s) 56, 120
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 2 cut(s) 277, 290
HpaII CCGG 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 154, 190
Hpy188III TCNNGA 1 cut(s) 62
Hpy8I GTNNAC 2 cut(s) 154, 190
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 2 cut(s) 17, 149
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 3 cut(s) 30, 72, 227
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
Hsp92I GRCGYC 2 cut(s) 56, 120
Kzo9I GATC 2 cut(s) 4, 159
LmnI GCTCC 1 cut(s) 179
LweI GCATC 1 cut(s) 17
MaeI CTAG 1 cut(s) 90
MalI GATC 2 cut(s) 6, 161
MboI GATC 2 cut(s) 4, 159
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 2 cut(s) 307, 325
MluI ACGCGT 1 cut(s) 66
MnlI CCTC 4 cut(s) 55, 69, 173, 270
MroXI GAANNNNTTC 1 cut(s) 276
MseI TTAA 4 cut(s) 50, 237, 303, 328
MspA1I CMGCKG 2 cut(s) 83, 110
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 295
MvaI CCWGG 1 cut(s) 295
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 41
NdeII GATC 2 cut(s) 4, 159
NlaIV GGNNCC 1 cut(s) 137
NmeAIII GCCGAG 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 276
PfeI GAWTC 2 cut(s) 277, 290
PkrI GCNGC 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 293
PspGI CCWGG 1 cut(s) 293
PspN4I GGNNCC 1 cut(s) 137
PsuI RGATCY 1 cut(s) 4
SaqAI TTAA 4 cut(s) 50, 237, 303, 328
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 4, 159
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 1 cut(s) 176
SetI ASST 4 cut(s) 137, 182, 237, 299
SfaNI GCATC 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 2 cut(s) 307, 325
SsiI CCGC 3 cut(s) 83, 108, 143
SspI AATATT 1 cut(s) 266
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 293
TaaI ACNGT 1 cut(s) 40
TasI AATT 2 cut(s) 307, 325
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 277, 290
Tru1I TTAA 4 cut(s) 50, 237, 303, 328
Tru9I TTAA 4 cut(s) 50, 237, 303, 328
TscAI CASTG 1 cut(s) 192
TspDTI ATGAA 1 cut(s) 302
TspRI CASTG 1 cut(s) 192
Vha464I CTTAAG 1 cut(s) 236
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 276
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.