FvH4_6g01013

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000189
Physical Location & Seq
Forward (+)
561252 .. 563205
1954 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01013.t1

Sequence Viewer

Length: 861 bp
ATGGAGAACACCGCGGTGATGATGGACTTGCCGGAAGGCTGCGTAGCCAACGTGATCGGGCTGACGACTCCTCGAGACGCGTGCAGGCTGTCGTTGATTTCGACGGATTTCAAGTCGGCCGCTGACTCCGACGCCGTTTGGGACAAGTTCCTTCCGCCTGATACTAATGAAATCGTATCCCACTCCGACTCTGAAATCGACGCCAAATCCAAGAAGGAGCTCTACTTCACTCTCTGTGATCCCATCCTCATCGATGATGGCAAGATGAGCTTTTCACTTGACAAATGGAGTGGGAAGAAATGCTATATGATAGCTGCAAGGAACCTTAGGATTGCTTGGGGTGATACTCCACGATATTGGACATGGGAATCTCTGCCTGAGTCCAGGTTCAAGGAGGCGGCGAAGCTTCGTGTTGTATGGTGGCTTGATATCCGTGGGAGAATTGAAACGCGGATGCTATCCCCATCGACGACATATAAAGCCTATCTGGTGTACAAGTTAGCTGAAGACACTCATGGACTACACCGGCCTGCTGTGGTCTCAGTTGGTACAAGAGCTTACGGGTTTGATTATGATGATGCTCGTTTGATGGAACTAACTGCAGACAATAAGGGGAATGTGGTTCTGGCGCCCCAAATAGCACTACAAAACAGGGAGTTGGAGAGGATGCCCGATCTCAGTGAAGCCAAATTTCCAAACGAAGGGAGAGGGGATGGGTGGCTGGAGATGGAGCTGGGTGAGTTTCACTGTCAAGGAGACGAAGACGGCGAGTTGGAAATGATCTGTTGTGAGGTTAAGACTAATATGAGCAAGAGTGGTCTCCTTGTTCAAGGGATTGAGGTCAGACCCGAAAGGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

287

Amino Acids

32.4

Weight (kDa)

4.88

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 99 - 283 4.3e-50 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 628
AccII CGCG 3 cut(s) 14, 80, 451
AciI CCGC 6 cut(s) 12, 14, 120, 155, 398, 451
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 117
AcsI RAATTY 1 cut(s) 689
AcuI CTGAAG 1 cut(s) 525
AcyI GRCGYC 3 cut(s) 132, 201, 629
AfaI GTAC 2 cut(s) 494, 550
AfiI CCNNNNNNNGG 1 cut(s) 701
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 4 cut(s) 112, 391, 446, 830
AjnI CCWGG 1 cut(s) 383
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 7 cut(s) 220, 270, 314, 406, 503, 557, 733
AluI AGCT 7 cut(s) 220, 270, 314, 406, 503, 557, 733
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 4 cut(s) 69, 544, 750, 824
AlwI GGATC 1 cut(s) 233
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 2 cut(s) 117, 527
ApeKI GCWGC 2 cut(s) 39, 314
ApoI RAATTY 1 cut(s) 689
AspLEI GCGC 1 cut(s) 631
AsuHPI GGTGA 3 cut(s) 28, 353, 749
AvaI CYCGRG 1 cut(s) 72
AxyI CCTNAGG 1 cut(s) 326
BanI GGYRCC 1 cut(s) 628
BanII GRGCYC 1 cut(s) 222
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 2 cut(s) 513, 768
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 26, 301
BccI CCATC 7 cut(s) 16, 251, 251, 472, 583, 707, 721
BceAI ACGGC 2 cut(s) 119, 781
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 1 cut(s) 385
BciVI GTATCC 1 cut(s) 187
BcoDI GTCTC 4 cut(s) 69, 544, 750, 824
BfmI CTRYAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 632
BfuI GTATCC 1 cut(s) 187
BisI GCNGC 4 cut(s) 40, 120, 315, 399
BlsI GCNGC 4 cut(s) 41, 121, 316, 400
Bme1390I CCNGG 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 72
BmiI GGNNCC 2 cut(s) 323, 630
BmrFI CCNGG 1 cut(s) 385
BmsI GCATC 3 cut(s) 444, 568, 657
BpiI GAAGAC 2 cut(s) 513, 768
BpmI CTGGAG 1 cut(s) 743
Bsa29I ATCGAT 1 cut(s) 252
BsaHI GRCGYC 3 cut(s) 132, 201, 629
BsaI GGTCTC 2 cut(s) 544, 824
BsaJI CCNNGG 2 cut(s) 12, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse118I RCCGGY 1 cut(s) 525
Bse21I CCTNAGG 1 cut(s) 326
BseBI CCWGG 1 cut(s) 385
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 12, 433
BseGI GGATG 4 cut(s) 243, 459, 672, 718
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMII CTCAG 3 cut(s) 369, 555, 691
BseRI GAGGAG 1 cut(s) 60
BseX3I CGGCCG 1 cut(s) 117
BseXI GCAGC 2 cut(s) 26, 301
BseYI CCCAGC 1 cut(s) 733
BsgI GTGCAG 1 cut(s) 103
Bsh1236I CGCG 3 cut(s) 14, 80, 451
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 119, 529
BshNI GGYRCC 1 cut(s) 628
BshVI ATCGAT 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 2 cut(s) 32, 526
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 4 cut(s) 69, 544, 750, 824
BsmBI CGTCTC 2 cut(s) 69, 750
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 2 cut(s) 119, 529
Bso31I GGTCTC 2 cut(s) 544, 824
BsoBI CYCGRG 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 222
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 4 cut(s) 54, 238, 673, 780
BspACI CCGC 6 cut(s) 12, 14, 120, 155, 398, 451
BspANI GGCC 2 cut(s) 119, 529
BspCNI CTCAG 3 cut(s) 370, 554, 690
BspDI ATCGAT 1 cut(s) 252
BspFNI CGCG 3 cut(s) 14, 80, 451
BspLI GGNNCC 2 cut(s) 323, 630
BspMAI CTGCAG 1 cut(s) 604
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 628
BspTNI GGTCTC 2 cut(s) 544, 824
BsrFI RCCGGY 1 cut(s) 525
BsrGI TGTACA 1 cut(s) 492
BssAI RCCGGY 1 cut(s) 525
BssECI CCNNGG 2 cut(s) 12, 433
BssMI GATC 4 cut(s) 54, 238, 673, 780
BssNI GRCGYC 3 cut(s) 132, 201, 629
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 1 cut(s) 749
BstACI GRCGYC 3 cut(s) 132, 201, 629
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 3 cut(s) 82, 86, 531
BstDEI CTNAG 4 cut(s) 326, 378, 541, 677
BstDSI CCRYGG 2 cut(s) 12, 433
BstF5I GGATG 4 cut(s) 243, 459, 672, 718
BstFNI CGCG 3 cut(s) 14, 80, 451
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 4 cut(s) 57, 241, 676, 783
BstMAI GTCTC 4 cut(s) 69, 544, 750, 824
BstMBI GATC 4 cut(s) 54, 238, 673, 780
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 1 cut(s) 267
BstNI CCWGG 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 1 cut(s) 600
BstUI CGCG 3 cut(s) 14, 80, 451
BstV1I GCAGC 2 cut(s) 26, 301
BstV2I GAAGAC 2 cut(s) 513, 768
BstXI CCANNNNNNTGG 1 cut(s) 357
BstZI CGGCCG 1 cut(s) 117
Bsu15I ATCGAT 1 cut(s) 252
Bsu36I CCTNAGG 1 cut(s) 326
BsuI GTATCC 1 cut(s) 187
BsuRI GGCC 2 cut(s) 119, 529
BsuTUI ATCGAT 1 cut(s) 252
BtgI CCRYGG 2 cut(s) 12, 433
BtsCI GGATG 4 cut(s) 243, 459, 672, 718
BtsIMutI CAGTG 2 cut(s) 685, 745
Cac8I GCNNGC 3 cut(s) 82, 86, 531
CfoI GCGC 1 cut(s) 631
Cfr10I RCCGGY 1 cut(s) 525
Cfr42I CCGCGG 1 cut(s) 15
ClaI ATCGAT 1 cut(s) 252
CseI GACGC 3 cut(s) 86, 140, 209
Csp6I GTAC 2 cut(s) 493, 549
CspCI CAANNNNNGTGG 2 cut(s) 271, 306
CviAII CATG 2 cut(s) 363, 515
CviQI GTAC 2 cut(s) 493, 549
DdeI CTNAG 4 cut(s) 326, 378, 541, 677
DinI GGCGCC 1 cut(s) 630
DpnI GATC 4 cut(s) 56, 240, 675, 782
DpnII GATC 4 cut(s) 54, 238, 673, 780
EaeI YGGCCR 1 cut(s) 117
EagI CGGCCG 1 cut(s) 117
EciI GGCGGA 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 220
EclXI CGGCCG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 222
Eco31I GGTCTC 2 cut(s) 544, 824
Eco32I GATATC 1 cut(s) 430
Eco52I CGGCCG 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 525
Eco81I CCTNAGG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 72
EcoICRI GAGCTC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 383
EcoRV GATATC 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 222
EgeI GGCGCC 1 cut(s) 630
EheI GGCGCC 1 cut(s) 630
Esp3I CGTCTC 2 cut(s) 69, 750
FaeI CATG 2 cut(s) 366, 518
FaiI YATR 9 cut(s) 306, 308, 364, 418, 475, 477, 516, 573, 806
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 155
FatI CATG 2 cut(s) 362, 514
Fnu4HI GCNGC 4 cut(s) 40, 120, 315, 399
FokI GGATG 4 cut(s) 230, 466, 679, 725
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 4 cut(s) 40, 120, 315, 399
GlaI GCGC 1 cut(s) 630
GluI GCNGC 4 cut(s) 40, 120, 315, 399
GsaI CCCAGC 1 cut(s) 737
GsuI CTGGAG 1 cut(s) 743
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 2 cut(s) 119, 529
HapII CCGG 2 cut(s) 32, 526
HgaI GACGC 3 cut(s) 86, 140, 209
HhaI GCGC 1 cut(s) 631
Hin1I GRCGYC 3 cut(s) 132, 201, 629
Hin1II CATG 2 cut(s) 366, 518
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 5 cut(s) 67, 125, 188, 368, 380
HpaII CCGG 2 cut(s) 32, 526
HphI GGTGA 3 cut(s) 28, 353, 749
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 4 cut(s) 130, 187, 193, 845
Hpy188III TCNNGA 1 cut(s) 74
Hpy8I GTNNAC 1 cut(s) 493
Hpy99I CGWCG 4 cut(s) 106, 134, 203, 472
HpyAV CCTTC 4 cut(s) 29, 161, 208, 695
HpyCH4III ACNGT 1 cut(s) 749
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 3 cut(s) 84, 317, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 267
HpyF3I CTNAG 4 cut(s) 326, 378, 541, 677
HpySE526I ACGT 1 cut(s) 51
Hsp92I GRCGYC 3 cut(s) 132, 201, 629
Hsp92II CATG 2 cut(s) 366, 518
HspAI GCGC 1 cut(s) 629
KasI GGCGCC 1 cut(s) 628
KspI CCGCGG 1 cut(s) 15
Kzo9I GATC 4 cut(s) 54, 238, 673, 780
LmnI GCTCC 2 cut(s) 217, 730
Lsp1109I GCAGC 2 cut(s) 26, 301
LweI GCATC 3 cut(s) 444, 568, 657
MaeII ACGT 1 cut(s) 51
MalI GATC 4 cut(s) 56, 240, 675, 782
MboI GATC 4 cut(s) 54, 238, 673, 780
MboII GAAGA 3 cut(s) 307, 518, 773
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 2 cut(s) 441, 689
MluI ACGCGT 1 cut(s) 78
Mly113I GGCGCC 1 cut(s) 629
MlyI GAGTC 4 cut(s) 61, 119, 182, 389
MmeI TCCRAC 4 cut(s) 153, 210, 639, 753
MnlI CCTC 7 cut(s) 81, 257, 388, 657, 701, 784, 832
MseI TTAA 1 cut(s) 795
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 2 cut(s) 14, 122
MspI CCGG 2 cut(s) 32, 526
MspR9I CCNGG 1 cut(s) 385
MvaI CCWGG 1 cut(s) 385
MvnI CGCG 3 cut(s) 14, 80, 451
MwoI GCNNNNNNNGC 1 cut(s) 267
NarI GGCGCC 1 cut(s) 629
NdeII GATC 4 cut(s) 54, 238, 673, 780
NlaIII CATG 2 cut(s) 366, 518
NlaIV GGNNCC 2 cut(s) 323, 630
OliI CACNNNNGTG 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 2 cut(s) 98, 765
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 4 cut(s) 41, 121, 316, 400
PleI GAGTC 4 cut(s) 61, 119, 182, 388
PluTI GGCGCC 1 cut(s) 632
PpsI GAGTC 4 cut(s) 61, 119, 182, 388
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 383
PspFI CCCAGC 1 cut(s) 733
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 2 cut(s) 323, 630
PstI CTGCAG 1 cut(s) 604
RsaI GTAC 2 cut(s) 494, 550
RsaNI GTAC 2 cut(s) 493, 549
RseI CAYNNNNRTG 1 cut(s) 14
SacI GAGCTC 1 cut(s) 222
SacII CCGCGG 1 cut(s) 15
SaqAI TTAA 1 cut(s) 795
SatI GCNGC 4 cut(s) 40, 120, 315, 399
Sau3AI GATC 4 cut(s) 54, 238, 673, 780
SchI GAGTC 4 cut(s) 61, 119, 182, 389
ScrFI CCNGG 1 cut(s) 385
SduI GDGCHC 1 cut(s) 222
SfaNI GCATC 3 cut(s) 444, 568, 657
SfcI CTRYAG 1 cut(s) 600
SfoI GGCGCC 1 cut(s) 630
Sfr274I CTCGAG 1 cut(s) 72
Sfr303I CCGCGG 1 cut(s) 15
SgrBI CCGCGG 1 cut(s) 15
SlaI CTCGAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 2 cut(s) 441, 689
SsiI CCGC 6 cut(s) 12, 14, 120, 155, 398, 451
SspDI GGCGCC 1 cut(s) 628
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 383
TaaI ACNGT 1 cut(s) 749
TaiI ACGT 1 cut(s) 54
TaqI TCGA 5 cut(s) 73, 101, 198, 252, 467
TasI AATT 2 cut(s) 441, 689
TatI WGTACW 1 cut(s) 492
TauI GCSGC 2 cut(s) 122, 401
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 1 cut(s) 795
Tru9I TTAA 1 cut(s) 795
TscAI CASTG 2 cut(s) 685, 752
TseI GCWGC 2 cut(s) 39, 314
TspDTI ATGAA 1 cut(s) 183
TspGWI ACGGA 2 cut(s) 119, 422
TspRI CASTG 2 cut(s) 685, 752
XapI RAATTY 1 cut(s) 689
XhoI CTCGAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.