Rmu_sc0003308.1_g000013

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003308.1
OG0000189
Physical Location & Seq
Forward (+)
52252 .. 53431
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003308.1_g000013.1.cds

Sequence Viewer

Length: 879 bp
atggagaacgcggcggtgatgatggacttgccggaaggctgcatagccaacgtgatcggcctaacaactcctcgagacgcgtgcaggctgtcgttgacttcgaaggatttcaagtcggcggcggactccgacgccgtttgggacaagttccttccgcctgataccagtgagatgctatcccgctccgaatctgcaatcgacgccaaatccaaaaaggagcttttcctcactctctctcacaaccccatcctcatcgacgatggcaagatgagcttttcacttgacaaatggagtgggaagaaatgctatatgatagctgcaaggcgccttcggattgcttggggtgatactcctaattattggacatggaaatctctgcccgagtccaggtttaaggaggctgtcgagcttcgtcttgtatgttggcttgaaatccgtgggagaattgagacacgcatgatgtccccatccacgacgtataaagcgtatctggtgtacaagttaacgggagacacttatggactacactggcctgccgtggtcacagttggtgcaagagcttatgggtttgattatgaatgccctggtttaaaggaaataactgcaggcattaagcgcacagtgtttctggcgccccaaagagtaatacaaagggagctggaagcaggaagaaggatgaggcccaaccacgaaatcagtgaagccaaatttccgaatgaagggaggggggatgggtggctggagatggagctgggtgagtttcactgtcaaggagacgaagacggggagttggaaatgatttgttgtgagattgagactggtatgagtaagaggggtctccttgttcaaggtattgaggtcagacccgagagggagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

292

Amino Acids

33.13

Weight (kDa)

5.63

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 324, 631
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 2 cut(s) 11, 80
AciI CCGC 6 cut(s) 11, 14, 119, 122, 155, 181
AcsI RAATTY 1 cut(s) 707
AcyI GRCGYC 4 cut(s) 132, 201, 325, 632
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 2 cut(s) 387, 719
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 3 cut(s) 112, 431, 848
AjnI CCWGG 2 cut(s) 386, 583
AluBI AGCT 7 cut(s) 220, 273, 317, 409, 560, 658, 751
AluI AGCT 7 cut(s) 220, 273, 317, 409, 560, 658, 751
Alw26I GTCTC 6 cut(s) 69, 443, 504, 768, 809, 842
Ama87I CYCGRG 3 cut(s) 72, 380, 866
AoxI GGCC 3 cut(s) 58, 530, 680
ApeKI GCWGC 2 cut(s) 39, 317
ApoI RAATTY 1 cut(s) 707
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 3 cut(s) 327, 618, 634
AspS9I GGNCC 1 cut(s) 681
AsuHPI GGTGA 3 cut(s) 28, 356, 767
AsuII TTCGAA 1 cut(s) 101
AvaI CYCGRG 3 cut(s) 72, 380, 866
BanI GGYRCC 2 cut(s) 324, 631
BbsI GAAGAC 1 cut(s) 786
BbvI GCAGC 2 cut(s) 26, 304
BccI CCATC 6 cut(s) 16, 254, 254, 475, 725, 739
BceAI ACGGC 2 cut(s) 119, 521
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 2 cut(s) 388, 585
BcoDI GTCTC 6 cut(s) 69, 443, 504, 768, 809, 842
BfmI CTRYAG 1 cut(s) 603
BfoI RGCGCY 2 cut(s) 328, 635
BisI GCNGC 4 cut(s) 12, 40, 120, 318
BlsI GCNGC 4 cut(s) 13, 41, 121, 319
Bme1390I CCNGG 2 cut(s) 388, 585
BmeT110I CYCGRG 3 cut(s) 72, 380, 866
BmgT120I GGNCC 1 cut(s) 681
BmiI GGNNCC 2 cut(s) 326, 633
BmrFI CCNGG 2 cut(s) 388, 585
BmsI GCATC 1 cut(s) 162
BpiI GAAGAC 1 cut(s) 786
BpmI CTGGAG 1 cut(s) 761
Bpu14I TTCGAA 1 cut(s) 101
BsaHI GRCGYC 4 cut(s) 132, 201, 325, 632
BsaI GGTCTC 1 cut(s) 842
BsaJI CCNNGG 3 cut(s) 436, 537, 583
Bsc4I CCNNNNNNNGG 2 cut(s) 387, 719
Bse1I ACTGG 3 cut(s) 165, 533, 823
BseBI CCWGG 2 cut(s) 388, 585
BseDI CCNNGG 3 cut(s) 436, 537, 583
BseGI GGATG 4 cut(s) 246, 467, 681, 736
BseLI CCNNNNNNNGG 2 cut(s) 387, 719
BseNI ACTGG 3 cut(s) 165, 533, 823
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 2 cut(s) 26, 304
BseYI CCCAGC 1 cut(s) 751
BsgI GTGCAG 1 cut(s) 103
Bsh1236I CGCG 2 cut(s) 11, 80
BshFI GGCC 3 cut(s) 60, 532, 682
BshNI GGYRCC 2 cut(s) 324, 631
BsiHKCI CYCGRG 3 cut(s) 72, 380, 866
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 2 cut(s) 155, 448
BslI CCNNNNNNNGG 2 cut(s) 387, 719
BsmAI GTCTC 6 cut(s) 69, 443, 504, 768, 809, 842
BsmBI CGTCTC 2 cut(s) 69, 768
BsmFI GGGAC 2 cut(s) 155, 448
BsmI GAATGC 1 cut(s) 584
BsnI GGCC 3 cut(s) 60, 532, 682
Bso31I GGTCTC 1 cut(s) 842
BsoBI CYCGRG 3 cut(s) 72, 380, 866
Bsp119I TTCGAA 1 cut(s) 101
Bsp1407I TGTACA 1 cut(s) 495
Bsp143I GATC 1 cut(s) 54
BspACI CCGC 6 cut(s) 11, 14, 119, 122, 155, 181
BspANI GGCC 3 cut(s) 60, 532, 682
BspFNI CGCG 2 cut(s) 11, 80
BspLI GGNNCC 2 cut(s) 326, 633
BspMAI CTGCAG 1 cut(s) 607
BspT104I TTCGAA 1 cut(s) 101
BspT107I GGYRCC 2 cut(s) 324, 631
BspTNI GGTCTC 1 cut(s) 842
BsrBI CCGCTC 1 cut(s) 183
BsrGI TGTACA 1 cut(s) 495
BsrI ACTGG 3 cut(s) 165, 533, 823
BssECI CCNNGG 3 cut(s) 436, 537, 583
BssMI GATC 1 cut(s) 54
BssNI GRCGYC 4 cut(s) 132, 201, 325, 632
Bst2UI CCWGG 2 cut(s) 388, 585
Bst4CI ACNGT 3 cut(s) 547, 622, 767
BstACI GRCGYC 4 cut(s) 132, 201, 325, 632
BstAUI TGTACA 1 cut(s) 495
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 4 cut(s) 82, 86, 534, 607
BstDSI CCRYGG 2 cut(s) 436, 537
BstF5I GGATG 4 cut(s) 246, 467, 681, 736
BstFNI CGCG 2 cut(s) 11, 80
BstH2I RGCGCY 2 cut(s) 328, 635
BstHHI GCGC 3 cut(s) 327, 618, 634
BstKTI GATC 1 cut(s) 57
BstMAI GTCTC 6 cut(s) 69, 443, 504, 768, 809, 842
BstMBI GATC 1 cut(s) 54
BstMWI GCNNNNNNNGC 3 cut(s) 200, 270, 615
BstNI CCWGG 2 cut(s) 388, 585
BstSCI CCNGG 2 cut(s) 386, 583
BstSFI CTRYAG 1 cut(s) 603
BstUI CGCG 2 cut(s) 11, 80
BstV1I GCAGC 2 cut(s) 26, 304
BstV2I GAAGAC 1 cut(s) 786
BsuRI GGCC 3 cut(s) 60, 532, 682
BtgI CCRYGG 2 cut(s) 436, 537
BtsCI GGATG 4 cut(s) 246, 467, 681, 736
BtsIMutI CAGTG 5 cut(s) 172, 526, 627, 703, 763
Cac8I GCNNGC 4 cut(s) 82, 86, 534, 607
CfoI GCGC 3 cut(s) 327, 618, 634
Cfr13I GGNCC 1 cut(s) 681
CseI GACGC 3 cut(s) 86, 140, 209
Csp6I GTAC 1 cut(s) 496
CspCI CAANNNNNGTGG 2 cut(s) 274, 309
CviAII CATG 2 cut(s) 366, 457
CviQI GTAC 1 cut(s) 496
DinI GGCGCC 2 cut(s) 326, 633
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
DraI TTTAAA 1 cut(s) 591
EciI GGCGGA 2 cut(s) 137, 144
Eco31I GGTCTC 1 cut(s) 842
Eco88I CYCGRG 3 cut(s) 72, 380, 866
EcoRII CCWGG 2 cut(s) 386, 583
EgeI GGCGCC 2 cut(s) 326, 633
EheI GGCGCC 2 cut(s) 326, 633
Esp3I CGTCTC 2 cut(s) 69, 768
FaeI CATG 2 cut(s) 369, 460
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 2 cut(s) 155, 448
FatI CATG 2 cut(s) 365, 456
FauI CCCGC 1 cut(s) 188
Fnu4HI GCNGC 4 cut(s) 12, 40, 120, 318
FokI GGATG 4 cut(s) 233, 454, 688, 743
Fsp4HI GCNGC 4 cut(s) 12, 40, 120, 318
GlaI GCGC 3 cut(s) 326, 617, 633
GluI GCNGC 4 cut(s) 12, 40, 120, 318
GsaI CCCAGC 1 cut(s) 755
GsuI CTGGAG 1 cut(s) 761
HaeII RGCGCY 2 cut(s) 328, 635
HaeIII GGCC 3 cut(s) 60, 532, 682
HapII CCGG 1 cut(s) 32
HgaI GACGC 3 cut(s) 86, 140, 209
HhaI GCGC 3 cut(s) 327, 618, 634
Hin1I GRCGYC 4 cut(s) 132, 201, 325, 632
Hin1II CATG 2 cut(s) 369, 460
Hin6I GCGC 3 cut(s) 325, 616, 632
HinP1I GCGC 3 cut(s) 325, 616, 632
HincII GTYRAC 2 cut(s) 96, 504
HindII GTYRAC 2 cut(s) 96, 504
HinfI GANTC 3 cut(s) 125, 188, 383
HpaI GTTAAC 1 cut(s) 504
HpaII CCGG 1 cut(s) 32
HphI GGTGA 3 cut(s) 28, 356, 767
Hpy166II GTNNAC 3 cut(s) 96, 496, 504
Hpy188I TCNGA 5 cut(s) 130, 187, 333, 714, 863
Hpy188III TCNNGA 1 cut(s) 74
Hpy8I GTNNAC 3 cut(s) 96, 496, 504
Hpy99I CGWCG 4 cut(s) 134, 203, 260, 478
HpyAV CCTTC 6 cut(s) 29, 97, 161, 338, 666, 713
HpyCH4III ACNGT 3 cut(s) 547, 622, 767
HpyCH4IV ACGT 2 cut(s) 51, 476
HpyCH4V TGCA 6 cut(s) 42, 84, 194, 320, 554, 605
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 270, 615
HpySE526I ACGT 2 cut(s) 51, 476
Hsp92I GRCGYC 4 cut(s) 132, 201, 325, 632
Hsp92II CATG 2 cut(s) 369, 460
HspAI GCGC 3 cut(s) 325, 616, 632
KasI GGCGCC 2 cut(s) 324, 631
KspAI GTTAAC 1 cut(s) 504
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 4 cut(s) 188, 217, 655, 748
Lsp1109I GCAGC 2 cut(s) 26, 304
LweI GCATC 1 cut(s) 162
MaeII ACGT 2 cut(s) 51, 476
MaeIII GTNAC 1 cut(s) 541
MalI GATC 1 cut(s) 56
MbiI CCGCTC 1 cut(s) 183
MboI GATC 1 cut(s) 54
MboII GAAGA 3 cut(s) 310, 681, 791
MluCI AATT 3 cut(s) 355, 444, 707
MluI ACGCGT 1 cut(s) 78
Mly113I GGCGCC 2 cut(s) 325, 632
MlyI GAGTC 2 cut(s) 119, 392
MmeI TCCRAC 2 cut(s) 153, 771
MnlI CCTC 9 cut(s) 81, 236, 260, 391, 672, 717, 825, 850, 864
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 4 cut(s) 393, 503, 590, 612
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 388, 585
Mva1269I GAATGC 1 cut(s) 584
MvaI CCWGG 2 cut(s) 388, 585
MvnI CGCG 2 cut(s) 11, 80
MwoI GCNNNNNNNGC 3 cut(s) 200, 270, 615
NarI GGCGCC 2 cut(s) 325, 632
NdeII GATC 1 cut(s) 54
NlaIII CATG 2 cut(s) 369, 460
NlaIV GGNNCC 2 cut(s) 326, 633
NmuCI GTSAC 1 cut(s) 541
NspV TTCGAA 1 cut(s) 101
PaeR7I CTCGAG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 2 cut(s) 98, 482
PctI GAATGC 1 cut(s) 584
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 4 cut(s) 13, 41, 121, 319
PleI GAGTC 2 cut(s) 119, 391
PluTI GGCGCC 2 cut(s) 328, 635
PpsI GAGTC 2 cut(s) 119, 391
Psp6I CCWGG 2 cut(s) 386, 583
PspFI CCCAGC 1 cut(s) 751
PspGI CCWGG 2 cut(s) 386, 583
PspN4I GGNNCC 2 cut(s) 326, 633
PspPI GGNCC 1 cut(s) 681
PstI CTGCAG 1 cut(s) 607
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
SaqAI TTAA 4 cut(s) 393, 503, 590, 612
SatI GCNGC 4 cut(s) 12, 40, 120, 318
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 1 cut(s) 681
SchI GAGTC 2 cut(s) 119, 392
ScrFI CCNGG 2 cut(s) 388, 585
SfaNI GCATC 1 cut(s) 162
SfcI CTRYAG 1 cut(s) 603
SfoI GGCGCC 2 cut(s) 326, 633
Sfr274I CTCGAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 101
SlaI CTCGAG 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 3 cut(s) 355, 444, 707
SsiI CCGC 6 cut(s) 11, 14, 119, 122, 155, 181
SspDI GGCGCC 2 cut(s) 324, 631
StyD4I CCNGG 2 cut(s) 386, 583
TaaI ACNGT 3 cut(s) 547, 622, 767
TaiI ACGT 2 cut(s) 54, 479
TaqI TCGA 5 cut(s) 73, 101, 198, 255, 405
TasI AATT 3 cut(s) 355, 444, 707
TatI WGTACW 1 cut(s) 495
TauI GCSGC 2 cut(s) 14, 122
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 4 cut(s) 393, 503, 590, 612
Tru9I TTAA 4 cut(s) 393, 503, 590, 612
TscAI CASTG 5 cut(s) 172, 533, 627, 703, 770
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 2 cut(s) 39, 317
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 2 cut(s) 591, 732
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 5 cut(s) 172, 533, 627, 703, 770
XapI RAATTY 1 cut(s) 707
XhoI CTCGAG 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.