MD04G1237100.v1.1

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000189
Physical Location & Seq
Reverse (-)
31562624 .. 31564127
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1237100.v1.1.491

Sequence Viewer

Length: 549 bp
ATGAGTTTTACTCTGGACAAATGGAGTGGGAAAAAATGTTACATGATATCGCCGAAGGACCTTATGATTGCTTGGGGTGGTACTCCTGCCTACTGGAAATGGATTTCTCTTGCCAACTCCAGGTTTGAGGAGGTGGCTGAGCTTGTAGTTGTTTGGTGGTTTGAGATCCGTGGCAAAATTGATACACGGATTCTTTCCCCCTCCACTATCTACAAAGCTTATCTTGTGTTCATGTTAAGTGAAAGGGCTAGAGGATTTGATCGTGACGATCCCTTGGAGGTCAAGGTAGGACTTTTTGGGGAAGAAGAGACTAGTAGTAAGCGCATTGTGTTTCTGGGGAGACAGAACATTACCGGAAGACCCATGGAAATCGATGAAACTCAATATCCAAAGAAAAGGCCCGACGGCTGGCTGGAGGTGGAGATTGGAGAGTTTTTCTGTCCAGGAGAAGAAGATGGGGGGTTAATGGAGATGACATGTATGGAGGTTGATAGAATGAAGAGAGGCCTTATTGTTCAGGGGATTGAAGTCAGGCCCAAAAGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

183

Amino Acids

21.18

Weight (kDa)

5.47

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 11 - 179 1.1e-51 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 160, 263
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 2 cut(s) 120, 408
AflIII ACRYGT 1 cut(s) 476
AgsI TTSAA 1 cut(s) 527
AhlI ACTAGT 1 cut(s) 311
AjnI CCWGG 2 cut(s) 119, 442
AluBI AGCT 2 cut(s) 142, 218
AluI AGCT 2 cut(s) 142, 218
Alw26I GTCTC 2 cut(s) 302, 334
AlwI GGATC 2 cut(s) 160, 263
AoxI GGCC 3 cut(s) 398, 505, 533
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 3 cut(s) 58, 399, 534
AvaII GGWCC 1 cut(s) 58
BbsI GAAGAC 1 cut(s) 364
BccI CCATC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 421
BciT130I CCWGG 2 cut(s) 121, 444
BcoDI GTCTC 2 cut(s) 302, 334
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 2 cut(s) 249, 312
BlpI GCTNAGC 1 cut(s) 138
Bme1390I CCNGG 2 cut(s) 121, 444
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 58, 399, 534
BmrFI CCNGG 2 cut(s) 121, 444
BpiI GAAGAC 1 cut(s) 364
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 2 cut(s) 103, 434
Bpu1102I GCTNAGC 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 372
BsaJI CCNNGG 3 cut(s) 169, 273, 363
BsaWI WCCGGW 1 cut(s) 353
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 408
Bse1I ACTGG 1 cut(s) 98
BseBI CCWGG 2 cut(s) 121, 444
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 3 cut(s) 169, 273, 363
BseLI CCNNNNNNNGG 2 cut(s) 120, 408
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 98
BseRI GAGGAG 1 cut(s) 143
BshFI GGCC 3 cut(s) 400, 507, 535
BshVI ATCGAT 1 cut(s) 372
BsiSI CCGG 1 cut(s) 354
BslI CCNNNNNNNGG 2 cut(s) 120, 408
BsmAI GTCTC 2 cut(s) 302, 334
BsnI GGCC 3 cut(s) 400, 507, 535
Bsp143I GATC 3 cut(s) 165, 259, 268
Bsp1720I GCTNAGC 1 cut(s) 138
Bsp19I CCATGG 1 cut(s) 363
BspANI GGCC 3 cut(s) 400, 507, 535
BspCNI CTCAG 1 cut(s) 130
BspDI ATCGAT 1 cut(s) 372
BspPI GGATC 2 cut(s) 160, 263
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 3 cut(s) 169, 273, 363
BssMI GATC 3 cut(s) 165, 259, 268
BssT1I CCWWGG 2 cut(s) 273, 363
Bst2UI CCWGG 2 cut(s) 121, 444
Bst6I CTCTTC 2 cut(s) 300, 494
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 1 cut(s) 138
BstDSI CCRYGG 2 cut(s) 169, 363
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 3 cut(s) 168, 262, 271
BstMAI GTCTC 2 cut(s) 302, 334
BstMBI GATC 3 cut(s) 165, 259, 268
BstNI CCWGG 2 cut(s) 121, 444
BstNSI RCATGY 1 cut(s) 480
BstSCI CCNGG 2 cut(s) 119, 442
BstV2I GAAGAC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
Bsu15I ATCGAT 1 cut(s) 372
BsuRI GGCC 3 cut(s) 400, 507, 535
BsuTUI ATCGAT 1 cut(s) 372
BtgI CCRYGG 2 cut(s) 169, 363
Cac8I GCNNGC 1 cut(s) 410
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 3 cut(s) 58, 399, 534
ClaI ATCGAT 1 cut(s) 372
Csp6I GTAC 1 cut(s) 81
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 4 cut(s) 43, 232, 364, 477
CviJI RGCY 9 cut(s) 137, 142, 218, 248, 400, 408, 412, 507, 535
CviKI_1 RGCY 9 cut(s) 137, 142, 218, 248, 400, 408, 412, 507, 535
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 1 cut(s) 138
DpnI GATC 3 cut(s) 167, 261, 270
DpnII GATC 3 cut(s) 165, 259, 268
Eam1104I CTCTTC 2 cut(s) 300, 494
EarI CTCTTC 2 cut(s) 300, 494
Eco130I CCWWGG 2 cut(s) 273, 363
Eco147I AGGCCT 1 cut(s) 507
Eco32I GATATC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 58
EcoRII CCWGG 2 cut(s) 119, 442
EcoRV GATATC 1 cut(s) 48
EcoT14I CCWWGG 2 cut(s) 273, 363
ErhI CCWWGG 2 cut(s) 273, 363
FaeI CATG 4 cut(s) 46, 235, 367, 480
FaiI YATR 6 cut(s) 44, 65, 233, 365, 478, 482
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 4 cut(s) 42, 231, 363, 476
FspBI CTAG 2 cut(s) 249, 312
GlaI GCGC 1 cut(s) 323
GsuI CTGGAG 2 cut(s) 103, 434
HaeIII GGCC 3 cut(s) 400, 507, 535
HapII CCGG 1 cut(s) 354
HhaI GCGC 1 cut(s) 324
Hin1II CATG 4 cut(s) 46, 235, 367, 480
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 1 cut(s) 190
HpaII CCGG 1 cut(s) 354
Hpy188III TCNNGA 2 cut(s) 14, 263
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 1 cut(s) 49
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 4 cut(s) 46, 235, 367, 480
HspAI GCGC 1 cut(s) 322
Kzo9I GATC 3 cut(s) 165, 259, 268
MaeI CTAG 2 cut(s) 249, 312
MaeIII GTNAC 2 cut(s) 38, 263
MalI GATC 3 cut(s) 167, 261, 270
MboI GATC 3 cut(s) 165, 259, 268
MboII GAAGA 6 cut(s) 314, 317, 369, 461, 464, 511
MflI RGATCY 1 cut(s) 165
MluCI AATT 1 cut(s) 177
MnlI CCTC 8 cut(s) 121, 124, 211, 245, 271, 409, 478, 497
MseI TTAA 2 cut(s) 236, 464
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 2 cut(s) 121, 444
MvaI CCWGG 2 cut(s) 121, 444
NcoI CCATGG 1 cut(s) 363
NdeII GATC 3 cut(s) 165, 259, 268
NlaIII CATG 4 cut(s) 46, 235, 367, 480
NmuCI GTSAC 1 cut(s) 263
NspI RCATGY 1 cut(s) 480
PceI AGGCCT 1 cut(s) 507
PciI ACATGT 1 cut(s) 476
PfeI GAWTC 1 cut(s) 190
PfoI TCCNGGA 1 cut(s) 442
PpuMI RGGWCCY 1 cut(s) 58
PscI ACATGT 1 cut(s) 476
Psp5II RGGWCCY 1 cut(s) 58
Psp6I CCWGG 2 cut(s) 119, 442
PspGI CCWGG 2 cut(s) 119, 442
PspPI GGNCC 3 cut(s) 58, 399, 534
PspPPI RGGWCCY 1 cut(s) 58
PsuI RGATCY 1 cut(s) 165
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 236, 464
Sau3AI GATC 3 cut(s) 165, 259, 268
Sau96I GGNCC 3 cut(s) 58, 399, 534
ScrFI CCNGG 2 cut(s) 121, 444
SetI ASST 9 cut(s) 63, 125, 135, 144, 220, 282, 288, 420, 489
SinI GGWCC 1 cut(s) 58
SpeI ACTAGT 1 cut(s) 311
Sse9I AATT 1 cut(s) 177
SseBI AGGCCT 1 cut(s) 507
SspMI CTAG 2 cut(s) 249, 312
StuI AGGCCT 1 cut(s) 507
StyD4I CCNGG 2 cut(s) 119, 442
StyI CCWWGG 2 cut(s) 273, 363
TaqI TCGA 1 cut(s) 372
TasI AATT 1 cut(s) 177
TfiI GAWTC 1 cut(s) 190
Tru1I TTAA 2 cut(s) 236, 464
Tru9I TTAA 2 cut(s) 236, 464
TseFI GTSAC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 3 cut(s) 220, 390, 512
TspGWI ACGGA 2 cut(s) 158, 202
VpaK11BI GGWCC 1 cut(s) 58
XceI RCATGY 1 cut(s) 480
XspI CTAG 2 cut(s) 249, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.