Rroxscaffold_6G00426730

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000189
Physical Location & Seq
Forward (+)
47407229 .. 47408735
1507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00426730.1

Sequence Viewer

Length: 780 bp
ATGATGGACTTGCCGGAAGGCTGCATAGCCAACGTGATCGGGCTGACGACTCCTCGAGACGCGTGCAGGCTGTCGTTGACTTCGATGGATTTCAAGTCGGCGGCAGACTCCGACGCCGTTTGGGACAAGTTCCTTCCGCCTGATACCAATGAGATCCTATCCCGTTCCGAATCTGCAATCGACGCCAAATCCAAGAAGGAGCTTTACCTCACTCTCTCTCACAACCCCATCCTCATCGACGATGGCAAGATGAGTTTTTCACTTGACAAATGGAGTGGGAAGAAATGCTATATGATAGCTGCAAGGCGCCTTCGGATTGCTTGGGATGATACTCCACGATATTGGAAATGGAAATCTCTGCCTGAGTCCAGGTTTAAGGAGGCGGTCAAGCTTCGTGTTGTATGTTGGCTTGAAATCCGTGGGAGAATCGAGACACGCATGCTGTCCCCATCCACGACGTATAAAGCGTATCTGGTGTACAAGTTGACGGGACACACTTATGGACTGCACTGGCCTGCCGTGGTCACAGTTGGTGCAAGAGCTTATGGGTTTGATTATGAATGCCCTGGTTTGAAGGAAATAACTGCAGGCATTAAGCGCACAGTGTTTCTGGCACCCCAAAGAGTAATACAAAGGGAGCTGGAAGCAGGAAGAAGGATGAGGCCCAACCAAGAAATCAGTGAAGCCAAATTTCCGAATGAAGGGAGGGGGATGGGTGGCTGGAGATGGAGCTGGGTGAGTTTCACTGTCAAGGAGACGAAGACGGGGAGTTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

259

Amino Acids

29.7

Weight (kDa)

9.5

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 94 - 259 6e-33 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80110 AT2G02240 AT2G02250 AT2G02280 AT2G02300 AT2G02310 AT2G02320 AT2G02340 AT2G02350 AT2G02350 AT2G02360
fragaria_vesca FvH4_1g04390 FvH4_4g27460 FvH4_6g01013 FvH4_6g01014 FvH4_6g01020 FvH4_6g01030 FvH4_6g01090 FvH4_6g01090 FvH4_6g01110
malus_domestica MD04G1236300.v1.1 MD04G1237000.v1.1 MD04G1237100.v1.1 MD04G1237300.v1.1 MD04G1237400.v1.1 MD04G1237600.v1.1 MD04G1237700.v1.1 MD04G1239300.v1.1 MD12G1255400.v1.1 MD12G1255500.v1.1 MD12G1255600.v1.1
prunus_persica Prupe.6G356300_v2.0.a1
pyrus_communis pycom04g21010 pycom04g21080 pycom04g21090 pycom12g23410 pycom12g23420 pycom12g23470
rosa_chinensis RchiOBHm_Chr2g0090041 RchiOBHm_Chr2g0105801 RchiOBHm_Chr2g0168101 RchiOBHm_Chr2g0168151 RchiOBHm_Chr3g0448461 RchiOBHm_Chr3g0448471 RchiOBHm_Chr3g0448501 RchiOBHm_Chr3g0448511 RchiOBHm_Chr3g0448521 RchiOBHm_Chr3g0448621 RchiOBHm_Chr3g0450261 RchiOBHm_Chr3g0450281 RchiOBHm_Chr6g0255241
rosa_laevigata RLG00000014851 RLG00000016080 RLG00000017458 RLG00000023974 RLG00000025742 RLG00000025744 RLG00000025883 RLG00000025900 RLG00000025901 RLG00000025903 RLG00000025904
rosa_multiflora Rmu_co8313739.1_g000001 Rmu_co8361343.1_g000001 Rmu_co8363919.1_g000001 Rmu_co8385575.1_g000001 Rmu_co8448943.1_g000001 Rmu_sc0000082.1_g000049 Rmu_sc0002741.1_g000008 Rmu_sc0003116.1_g000039 Rmu_sc0003308.1_g000013 Rmu_sc0005298.1_g000006 Rmu_sc0005298.1_g000008 Rmu_sc0007766.1_g000003 Rmu_sc0011979.1_g000006 Rmu_sc0016252.1_g000001 Rmu_sc0023110.1_g000001 Rmu_sc0023110.1_g000002 Rmu_sc0023110.1_g000003 Rmu_sc0041893.1_g000001
rosa_roxburghii Rroxscaffold_2G00137910 Rroxscaffold_2G00151450 Rroxscaffold_6G00426700 Rroxscaffold_6G00426730 Rroxscaffold_6G00426740 Rroxscaffold_6G00426750 Rroxscaffold_6G00426760 Rroxscaffold_6G00426910 Rroxscaffold_6G00428000 Rroxscaffold_6G00428010
rosa_rugosa Rorug02G0004900 Rorug02G0130400 Rorug02G0130400 Rorug02G0612700 Rorug02G0612900 Rorug02G0613000 Rorug02G0613100 Rorug02G0613100 Rorug02G0614700 Rorug02G0614800 Rorug02G0614900 Rorug02G0626500
rosa_samantha Rh2BG049100 Rh2BG190800 Rh2BG613500 Rh2BG613800 Rh2CG051300 Rh2DG050700 Rh2DG187600 Rh2DG187700 Rh2DG402600 Rh3AG012100 Rh3AG012200 Rh3AG012300 Rh3AG012500 Rh3AG012600 Rh3AG012700 Rh3AG012800 Rh3AG013700 Rh3AG025900 Rh3AG026200 Rh3AG026500 Rh3AG026700 Rh3BG011900 Rh3BG012000 Rh3BG012100 Rh3BG012200 Rh3BG012300 Rh3BG013500 Rh3BG026600 Rh3BG026800 Rh3BG027200 Rh3BG027400 Rh3DG012300 Rh3DG012400 Rh3DG012500 Rh3DG012600 Rh3DG012700 Rh3DG012800 Rh3DG012900 Rh3DG014300 Rh3DG026500 Rh3DG026800 Rh3DG027100 Rh3DG027200 Rh3DG122000 Rh3DG282500 Rh5BG266700 Rh6BG370500 Rh7CG395700
rosa_wichuraiana Rw0G020400 Rw2G004010 Rw2G014220 Rw3G000940 Rw3G000950 Rw3G000960 Rw3G001010 Rw3G002090 Rw3G002100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 306, 613
AccII CGCG 1 cut(s) 62
AciI CCGC 3 cut(s) 101, 137, 383
AclWI GGATC 1 cut(s) 148
AcsI RAATTY 1 cut(s) 689
AcyI GRCGYC 3 cut(s) 114, 183, 307
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 701
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 3 cut(s) 94, 413, 574
AjnI CCWGG 2 cut(s) 368, 565
AluBI AGCT 6 cut(s) 202, 299, 391, 542, 640, 732
AluI AGCT 6 cut(s) 202, 299, 391, 542, 640, 732
Alw26I GTCTC 3 cut(s) 51, 425, 749
AlwI GGATC 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 54
AoxI GGCC 2 cut(s) 512, 662
ApeKI GCWGC 2 cut(s) 21, 299
ApoI RAATTY 1 cut(s) 689
AspLEI GCGC 2 cut(s) 309, 600
AspS9I GGNCC 1 cut(s) 663
AsuHPI GGTGA 1 cut(s) 748
AvaI CYCGRG 1 cut(s) 54
BanI GGYRCC 2 cut(s) 306, 613
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BbsI GAAGAC 1 cut(s) 767
BbvI GCAGC 2 cut(s) 8, 286
BccI CCATC 6 cut(s) 79, 236, 236, 457, 706, 720
BceAI ACGGC 2 cut(s) 101, 503
BcgI CGANNNNNNTGC 2 cut(s) 45, 79
BciT130I CCWGG 2 cut(s) 370, 567
BcoDI GTCTC 3 cut(s) 51, 425, 749
BfmI CTRYAG 1 cut(s) 585
BfoI RGCGCY 1 cut(s) 310
BisI GCNGC 3 cut(s) 22, 102, 300
BlsI GCNGC 3 cut(s) 23, 103, 301
Bme1390I CCNGG 2 cut(s) 370, 567
BmeT110I CYCGRG 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 663
BmiI GGNNCC 2 cut(s) 308, 615
BmrFI CCNGG 2 cut(s) 370, 567
BpiI GAAGAC 1 cut(s) 767
BpmI CTGGAG 1 cut(s) 742
BsaHI GRCGYC 3 cut(s) 114, 183, 307
BsaJI CCNNGG 3 cut(s) 418, 519, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse1I ACTGG 1 cut(s) 515
BseBI CCWGG 2 cut(s) 370, 567
BseDI CCNNGG 3 cut(s) 418, 519, 565
BseGI GGATG 5 cut(s) 228, 331, 449, 663, 717
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 515
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 8, 286
BseYI CCCAGC 1 cut(s) 732
BsgI GTGCAG 2 cut(s) 85, 491
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 2 cut(s) 514, 664
BshNI GGYRCC 2 cut(s) 306, 613
BsiHKCI CYCGRG 1 cut(s) 54
BsiSI CCGG 1 cut(s) 14
BslFI GGGAC 3 cut(s) 137, 430, 504
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 3 cut(s) 51, 425, 749
BsmBI CGTCTC 2 cut(s) 51, 749
BsmFI GGGAC 3 cut(s) 137, 430, 504
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 2 cut(s) 514, 664
BsoBI CYCGRG 1 cut(s) 54
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 2 cut(s) 36, 153
BspACI CCGC 3 cut(s) 101, 137, 383
BspANI GGCC 2 cut(s) 514, 664
BspCNI CTCAG 1 cut(s) 355
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 2 cut(s) 308, 615
BspMAI CTGCAG 1 cut(s) 589
BspPI GGATC 1 cut(s) 148
BspT107I GGYRCC 2 cut(s) 306, 613
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 1 cut(s) 515
BssECI CCNNGG 3 cut(s) 418, 519, 565
BssMI GATC 2 cut(s) 36, 153
BssNI GRCGYC 3 cut(s) 114, 183, 307
Bst2UI CCWGG 2 cut(s) 370, 567
Bst4CI ACNGT 3 cut(s) 529, 604, 748
BstACI GRCGYC 3 cut(s) 114, 183, 307
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 5 cut(s) 64, 68, 440, 516, 589
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 2 cut(s) 418, 519
BstF5I GGATG 5 cut(s) 228, 331, 449, 663, 717
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 310
BstHHI GCGC 2 cut(s) 309, 600
BstKTI GATC 2 cut(s) 39, 156
BstMAI GTCTC 3 cut(s) 51, 425, 749
BstMBI GATC 2 cut(s) 36, 153
BstMWI GCNNNNNNNGC 2 cut(s) 182, 597
BstNI CCWGG 2 cut(s) 370, 567
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 2 cut(s) 368, 565
BstSFI CTRYAG 1 cut(s) 585
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 2 cut(s) 8, 286
BstV2I GAAGAC 1 cut(s) 767
BstX2I RGATCY 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 342
BstYI RGATCY 1 cut(s) 153
BsuRI GGCC 2 cut(s) 514, 664
BtgI CCRYGG 2 cut(s) 418, 519
BtsCI GGATG 5 cut(s) 228, 331, 449, 663, 717
BtsIMutI CAGTG 4 cut(s) 508, 609, 685, 744
Cac8I GCNNGC 5 cut(s) 64, 68, 440, 516, 589
CfoI GCGC 2 cut(s) 309, 600
Cfr13I GGNCC 1 cut(s) 663
CseI GACGC 3 cut(s) 68, 122, 191
Csp6I GTAC 1 cut(s) 478
CspCI CAANNNNNGTGG 2 cut(s) 256, 291
CviAII CATG 1 cut(s) 439
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 1 cut(s) 363
DinI GGCGCC 1 cut(s) 308
DpnI GATC 2 cut(s) 38, 155
DpnII GATC 2 cut(s) 36, 153
EciI GGCGGA 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 54
EcoRII CCWGG 2 cut(s) 368, 565
EgeI GGCGCC 1 cut(s) 308
EheI GGCGCC 1 cut(s) 308
Esp3I CGTCTC 2 cut(s) 51, 749
FaeI CATG 1 cut(s) 442
FaiI YATR 9 cut(s) 26, 291, 293, 403, 440, 462, 501, 546, 558
FaqI GGGAC 3 cut(s) 137, 430, 504
FatI CATG 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 22, 102, 300
FokI GGATG 5 cut(s) 215, 338, 436, 670, 724
Fsp4HI GCNGC 3 cut(s) 22, 102, 300
GlaI GCGC 2 cut(s) 308, 599
GluI GCNGC 3 cut(s) 22, 102, 300
GsaI CCCAGC 1 cut(s) 736
GsuI CTGGAG 1 cut(s) 742
HaeII RGCGCY 1 cut(s) 310
HaeIII GGCC 2 cut(s) 514, 664
HapII CCGG 1 cut(s) 14
HgaI GACGC 3 cut(s) 68, 122, 191
HhaI GCGC 2 cut(s) 309, 600
Hin1I GRCGYC 3 cut(s) 114, 183, 307
Hin1II CATG 1 cut(s) 442
Hin6I GCGC 2 cut(s) 307, 598
HinP1I GCGC 2 cut(s) 307, 598
HincII GTYRAC 2 cut(s) 78, 486
HindII GTYRAC 2 cut(s) 78, 486
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 5 cut(s) 49, 107, 170, 365, 426
HpaII CCGG 1 cut(s) 14
HphI GGTGA 1 cut(s) 748
Hpy166II GTNNAC 3 cut(s) 78, 478, 486
Hpy188I TCNGA 4 cut(s) 112, 169, 315, 696
Hpy188III TCNNGA 2 cut(s) 56, 430
Hpy8I GTNNAC 3 cut(s) 78, 478, 486
Hpy99I CGWCG 4 cut(s) 116, 185, 242, 460
HpyAV CCTTC 7 cut(s) 11, 143, 190, 320, 568, 648, 695
HpyCH4III ACNGT 3 cut(s) 529, 604, 748
HpyCH4IV ACGT 2 cut(s) 33, 458
HpyCH4V TGCA 7 cut(s) 24, 66, 176, 302, 508, 536, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 597
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 33, 458
Hsp92I GRCGYC 3 cut(s) 114, 183, 307
Hsp92II CATG 1 cut(s) 442
HspAI GCGC 2 cut(s) 307, 598
KasI GGCGCC 1 cut(s) 306
Kzo9I GATC 2 cut(s) 36, 153
LmnI GCTCC 3 cut(s) 199, 637, 729
Lsp1109I GCAGC 2 cut(s) 8, 286
MaeII ACGT 2 cut(s) 33, 458
MaeIII GTNAC 1 cut(s) 523
MalI GATC 2 cut(s) 38, 155
MboI GATC 2 cut(s) 36, 153
MboII GAAGA 3 cut(s) 292, 663, 772
MflI RGATCY 1 cut(s) 153
MluCI AATT 1 cut(s) 689
MluI ACGCGT 1 cut(s) 60
Mly113I GGCGCC 1 cut(s) 307
MlyI GAGTC 3 cut(s) 43, 101, 374
MmeI TCCRAC 2 cut(s) 135, 752
MnlI CCTC 6 cut(s) 63, 218, 242, 373, 654, 699
MseI TTAA 2 cut(s) 375, 594
MslI CAYNNNNRTG 1 cut(s) 498
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 2 cut(s) 370, 567
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 2 cut(s) 370, 567
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 2 cut(s) 182, 597
NarI GGCGCC 1 cut(s) 307
NdeII GATC 2 cut(s) 36, 153
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 2 cut(s) 308, 615
NmuCI GTSAC 1 cut(s) 523
NspI RCATGY 1 cut(s) 442
PaeI GCATGC 1 cut(s) 442
PaeR7I CTCGAG 1 cut(s) 54
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 464
PctI GAATGC 1 cut(s) 566
PfeI GAWTC 2 cut(s) 170, 426
PkrI GCNGC 3 cut(s) 23, 103, 301
PleI GAGTC 3 cut(s) 43, 101, 373
PluTI GGCGCC 1 cut(s) 310
PpsI GAGTC 3 cut(s) 43, 101, 373
Psp6I CCWGG 2 cut(s) 368, 565
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 2 cut(s) 368, 565
PspN4I GGNNCC 2 cut(s) 308, 615
PspPI GGNCC 1 cut(s) 663
PstI CTGCAG 1 cut(s) 589
PsuI RGATCY 1 cut(s) 153
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
RseI CAYNNNNRTG 1 cut(s) 498
SaqAI TTAA 2 cut(s) 375, 594
SatI GCNGC 3 cut(s) 22, 102, 300
Sau3AI GATC 2 cut(s) 36, 153
Sau96I GGNCC 1 cut(s) 663
SchI GAGTC 3 cut(s) 43, 101, 374
ScrFI CCNGG 2 cut(s) 370, 567
SfcI CTRYAG 1 cut(s) 585
SfoI GGCGCC 1 cut(s) 308
Sfr274I CTCGAG 1 cut(s) 54
SlaI CTCGAG 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
SphI GCATGC 1 cut(s) 442
Sse9I AATT 1 cut(s) 689
SsiI CCGC 3 cut(s) 101, 137, 383
SspDI GGCGCC 1 cut(s) 306
StyD4I CCNGG 2 cut(s) 368, 565
TaaI ACNGT 3 cut(s) 529, 604, 748
TaiI ACGT 2 cut(s) 36, 461
TaqI TCGA 5 cut(s) 55, 83, 180, 237, 429
TasI AATT 1 cut(s) 689
TatI WGTACW 1 cut(s) 477
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 2 cut(s) 170, 426
Tru1I TTAA 2 cut(s) 375, 594
Tru9I TTAA 2 cut(s) 375, 594
TscAI CASTG 4 cut(s) 515, 609, 685, 751
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 2 cut(s) 21, 299
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 2 cut(s) 573, 714
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 4 cut(s) 515, 609, 685, 751
XapI RAATTY 1 cut(s) 689
XceI RCATGY 1 cut(s) 442
XhoI CTCGAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.