MD04G1174600.v1.1

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000244
Physical Location & Seq
Forward (+)
26601576 .. 26604044
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1174600.v1.1.491

Sequence Viewer

Length: 879 bp
ATGTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGACTCATTTTTTAAGTCACTTTTTGAGTTAAAGTCGAAAAATTTGGACCTTTTTCTTATTGGGGAAACTATTCATAACCCAGTTGAAGCTTACTGGTTTGCTGCCTGTTGGCCTTATGTAGAGTTTCTTCACATGAAACCTGCGAAAGGGACCGCATTTGCAAGGACCAAAATGCGCAATTATGCATCAGGAAACACTGTTGAGAAAACATTTCGAGCAGGCAGCACGATCAATGAGGCAAATATATACAAGGAAACAAAGCAATTCGTATACAAAGACGGTCCCCAGTTTGTCTTCATGGACCTGACTACCTATGAAGAAACTCGTGTAAATGAATCGAGTATTGGGGACAAAACAAAGTGGCTGAAAGAGGGAATGGACTGCAATTTGCTTCTTTGGAATGATAAACTTATCGATGTTGATATCCCCATCACAGTGCAGCTGAAGGTGGTTGATGTTGATCCTGGACTTAAAGGTGACACAGCTCAAGACGGGACGGAACGGAATGGATGGGACGGGACGGGACGGAACAAAGAGGGAGCAAAGATACTCTCGGATGGAAACAAGGAGGAAGAAGGAGACGTAGAGGTTATAATTTTGTGTTCCACGGAAGTGGAACGAGTCGTTCCAGGGCGAGAGGCGGAACAAAAATTCACCAAAAACTCGTCCCGTGGAACAACGCCTTCCACCAGTTTTAGGCGCACCAAACATGGGACGCAACGTCTCGTCCCACTCCGTTCCGTCCCGTCCCACGTACCAAACGGTACCTTAAATTCACATGTGTTCTTGCCCAGGAGGTGGAACAAAGCGAGCGACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFP EFP_N Elong-fact-P_C

Protein Analysis

293

Amino Acids

32.62

Weight (kDa)

6.98

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFP_N PF08207 59 - 95 1.8e-11 Elongation factor P (EF-P) KOW-like domain
EFP PF01132 107 - 157 2e-19 Elongation factor P (EF-P) OB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000244)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02550 AT4G02550 AT4G02550 AT4G02550 AT4G02550
fragaria_vesca FvH4_1g20120 FvH4_2g13781 FvH4_4g15662 FvH4_6g32032
malus_domestica MD02G1272200.v1.1 MD04G1174600.v1.1 MD07G1041200.v1.1 MD07G1064000.v1.1
prunus_persica Prupe.1G245200_v2.0.a1 Prupe.1G287900_v2.0.a1 Prupe.2G077600_v2.0.a1 Prupe.2G078000_v2.0.a1 Prupe.3G281200_v2.0.a1 Prupe.4G256700_v2.0.a1 Prupe.5G014200_v2.0.a1 Prupe.5G014400_v2.0.a1 Prupe.5G049300_v2.0.a1 Prupe.5G049400_v2.0.a1 Prupe.6G167700_v2.0.a1 Prupe.7G027800_v2.0.a1
pyrus_communis pycom02g23280 pycom07g02860 pycom07g04940 pycom07g04960 pycom07g04980
rosa_chinensis RchiOBHm_Chr1g0330381 RchiOBHm_Chr2g0139691 RchiOBHm_Chr2g0145201 RchiOBHm_Chr3g0485151 RchiOBHm_Chr4g0405761 RchiOBHm_Chr4g0417571 RchiOBHm_Chr5g0022261 RchiOBHm_Chr5g0053961 RchiOBHm_Chr6g0298071 RchiOBHm_Chr7g0231381
rosa_laevigata RLG00000002919 RLG00000009166 RLG00000013803 RLG00000013816 RLG00000015126 RLG00000017423 RLG00000019811 RLG00000019826 RLG00000028831 RLG00000029802 RLG00000029840 RLG00000029955 RLG00000029956 RLG00000034858
rosa_multiflora Rmu_co8446275.1_g000001 Rmu_sc0000212.1_g000021 Rmu_sc0000945.1_g000025 Rmu_sc0001512.1_g000009 Rmu_sc0003482.1_g000004 Rmu_sc0004039.1_g000001 Rmu_sc0004390.1_g000008 Rmu_sc0004711.1_g000022 Rmu_sc0005887.1_g000001 Rmu_sc0007795.1_g000002 Rmu_sc0008148.1_g000034 Rmu_sc0008927.1_g000001 Rmu_sc0009714.1_g000007 Rmu_sc0009806.1_g000002 Rmu_sc0012995.1_g000005 Rmu_sc0014278.1_g000005 Rmu_sc0023501.1_g000002
rosa_roxburghii Rroxscaffold_2G00105280 Rroxscaffold_2G00111200 Rroxscaffold_2G00117910 Rroxscaffold_3G00235020 Rroxscaffold_5G00350240 Rroxscaffold_5G00381630 Rroxscaffold_6G00403970 Rroxscaffold_7G00158580
rosa_rugosa Rorug01G0012500 Rorug02G0357800 Rorug02G0359000 Rorug02G0359100 Rorug02G0359200 Rorug04G0061200 Rorug04G0162600 Rorug05G0271800 Rorug06G0213500 Rorug06G0213600 Rorug06G0332900 Rorug07G0148200
rosa_samantha Rh1AG259900 Rh1AG410300 Rh2AG397400 Rh2AG397500 Rh2AG409000 Rh2BG187200 Rh2BG417700 Rh2BG419500 Rh2BG460700 Rh2CG384200 Rh2CG384300 Rh2CG395100 Rh2DG065900 Rh2DG428800 Rh3CG301800 Rh3DG242800 Rh4BG141800 Rh4CG022100 Rh5BG548900 Rh5CG571800 Rh6AG328800 Rh6CG021500 Rh6CG066400 Rh6CG237200 Rh6DG021700 Rh6DG063300 Rh7AG131700 Rh7BG051800 Rh7CG385100 Rh7DG025500 Rh7DG025600
rosa_wichuraiana Rw0G007310 Rw0G016860 Rw2G029630 Rw2G033470 Rw2G034500 Rw3G014930 Rw3G019520 Rw4G010080 Rw4G016450 Rw5G023350 Rw5G041160 Rw5G048810 Rw6G006750 Rw6G017100 Rw7G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 650
Acc16I TGCGCA 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 205
Acc65I GGTACC 1 cut(s) 821
AccB1I GGYRCC 1 cut(s) 821
AccB7I CCANNNNNTGG 1 cut(s) 855
AccI GTMKAC 1 cut(s) 327
AciI CCGC 2 cut(s) 210, 698
AclWI GGATC 1 cut(s) 512
AcsI RAATTY 3 cut(s) 97, 707, 829
AcuI CTGAAG 1 cut(s) 521
AfaI GTAC 2 cut(s) 813, 823
AfiI CCNNNNNNNGG 4 cut(s) 203, 753, 768, 855
AflIII ACRYGT 1 cut(s) 835
AgsI TTSAA 1 cut(s) 143
AhdI GACNNNNNGTC 1 cut(s) 777
AjnI CCWGG 3 cut(s) 520, 685, 848
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AleI CACNNNNGTG 1 cut(s) 668
AluBI AGCT 3 cut(s) 146, 499, 542
AluI AGCT 3 cut(s) 146, 499, 542
Alw26I GTCTC 2 cut(s) 630, 785
AlwI GGATC 1 cut(s) 512
AoxI GGCC 1 cut(s) 167
ApeKI GCWGC 3 cut(s) 158, 279, 496
ApoI RAATTY 3 cut(s) 97, 707, 829
Asp700I GAANNNNTTC 1 cut(s) 126
Asp718I GGTACC 1 cut(s) 821
AspLEI GCGC 2 cut(s) 234, 759
AspS9I GGNCC 5 cut(s) 103, 207, 222, 338, 358
AsuHPI GGTGA 2 cut(s) 545, 703
AvaII GGWCC 5 cut(s) 103, 207, 222, 338, 358
BanI GGYRCC 1 cut(s) 821
BarI GAAGNNNNNNTAC 2 cut(s) 168, 200
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 343
BbvI GCAGC 3 cut(s) 145, 291, 508
BccI CCATC 3 cut(s) 494, 561, 608
BciT130I CCWGG 3 cut(s) 522, 687, 850
BcoDI GTCTC 2 cut(s) 630, 785
BfuAI ACCTGC 1 cut(s) 205
BisI GCNGC 3 cut(s) 159, 280, 497
BlsI GCNGC 3 cut(s) 160, 281, 498
Bme1390I CCNGG 3 cut(s) 522, 687, 850
Bme18I GGWCC 5 cut(s) 103, 207, 222, 338, 358
BmeRI GACNNNNNGTC 1 cut(s) 777
BmgT120I GGNCC 5 cut(s) 103, 207, 222, 338, 358
BmiI GGNNCC 3 cut(s) 208, 340, 823
BmrFI CCNGG 3 cut(s) 522, 687, 850
BmrI ACTGGG 2 cut(s) 131, 337
BmsI GCATC 1 cut(s) 251
BmuI ACTGGG 2 cut(s) 131, 337
BpiI GAAGAC 1 cut(s) 343
BpuEI CTTGAG 1 cut(s) 528
Bsa29I ATCGAT 1 cut(s) 471
BsaAI YACGTR 1 cut(s) 811
BsaBI GATNNNNATC 1 cut(s) 516
BsaJI CCNNGG 4 cut(s) 663, 686, 727, 848
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 753, 768, 855
Bse1I ACTGG 4 cut(s) 137, 155, 343, 747
Bse8I GATNNNNATC 1 cut(s) 516
BseBI CCWGG 3 cut(s) 522, 687, 850
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 4 cut(s) 663, 686, 727, 848
BseGI GGATG 2 cut(s) 572, 619
BseJI GATNNNNATC 1 cut(s) 516
BseLI CCNNNNNNNGG 4 cut(s) 203, 753, 768, 855
BseNI ACTGG 4 cut(s) 137, 155, 343, 747
BseXI GCAGC 3 cut(s) 145, 291, 508
BsgI GTGCAG 1 cut(s) 515
BshFI GGCC 1 cut(s) 169
BshNI GGYRCC 1 cut(s) 821
BshVI ATCGAT 1 cut(s) 471
BslI CCNNNNNNNGG 4 cut(s) 203, 753, 768, 855
BsmAI GTCTC 2 cut(s) 630, 785
BsmBI CGTCTC 2 cut(s) 630, 785
BsnI GGCC 1 cut(s) 169
Bsp143I GATC 2 cut(s) 285, 517
BspACI CCGC 2 cut(s) 210, 698
BspANI GGCC 1 cut(s) 169
BspDI ATCGAT 1 cut(s) 471
BspLI GGNNCC 3 cut(s) 208, 340, 823
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 821
BsrI ACTGG 4 cut(s) 137, 155, 343, 747
BssECI CCNNGG 4 cut(s) 663, 686, 727, 848
BssMI GATC 2 cut(s) 285, 517
BssNAI GTATAC 1 cut(s) 328
BssSI CACGAG 1 cut(s) 381
Bst1107I GTATAC 1 cut(s) 328
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 3 cut(s) 522, 687, 850
Bst4CI ACNGT 4 cut(s) 256, 338, 493, 821
BstBAI YACGTR 1 cut(s) 811
BstC8I GCNNGC 2 cut(s) 277, 868
BstDSI CCRYGG 2 cut(s) 663, 727
BstENI CCTNNNNNAGG 1 cut(s) 201
BstF5I GGATG 2 cut(s) 572, 619
BstHHI GCGC 2 cut(s) 234, 759
BstKTI GATC 2 cut(s) 288, 520
BstMAI GTCTC 2 cut(s) 630, 785
BstMBI GATC 2 cut(s) 285, 517
BstNI CCWGG 3 cut(s) 522, 687, 850
BstNSI RCATGY 1 cut(s) 839
BstSCI CCNGG 3 cut(s) 520, 685, 848
BstV1I GCAGC 3 cut(s) 145, 291, 508
BstV2I GAAGAC 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 670
BstZ17I GTATAC 1 cut(s) 328
Bsu15I ATCGAT 1 cut(s) 471
BsuRI GGCC 1 cut(s) 169
BsuTUI ATCGAT 1 cut(s) 471
BtgI CCRYGG 2 cut(s) 663, 727
BtsCI GGATG 2 cut(s) 572, 619
BtsIMutI CAGTG 2 cut(s) 252, 498
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 2 cut(s) 277, 868
CfoI GCGC 2 cut(s) 234, 759
Cfr13I GGNCC 5 cut(s) 103, 207, 222, 338, 358
ClaI ATCGAT 1 cut(s) 471
CseI GACGC 1 cut(s) 781
Csp6I GTAC 2 cut(s) 812, 822
CviAII CATG 4 cut(s) 190, 355, 767, 836
CviJI RGCY 5 cut(s) 146, 169, 421, 499, 542
CviKI_1 RGCY 5 cut(s) 146, 169, 421, 499, 542
CviQI GTAC 2 cut(s) 812, 822
DpnI GATC 2 cut(s) 287, 519
DpnII GATC 2 cut(s) 285, 517
DriI GACNNNNNGTC 1 cut(s) 777
Eam1105I GACNNNNNGTC 1 cut(s) 777
EciI GGCGGA 1 cut(s) 713
Eco32I GATATC 1 cut(s) 481
Eco47I GGWCC 5 cut(s) 103, 207, 222, 338, 358
Eco57I CTGAAG 1 cut(s) 521
EcoNI CCTNNNNNAGG 1 cut(s) 201
EcoRII CCWGG 3 cut(s) 520, 685, 848
EcoRV GATATC 1 cut(s) 481
EcoT22I ATGCAT 1 cut(s) 244
Esp3I CGTCTC 2 cut(s) 630, 785
FaeI CATG 4 cut(s) 193, 358, 770, 839
FatI CATG 4 cut(s) 189, 354, 766, 835
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 3 cut(s) 159, 280, 497
FokI GGATG 2 cut(s) 579, 626
Fsp4HI GCNGC 3 cut(s) 159, 280, 497
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 2 cut(s) 233, 758
GluI GCNGC 3 cut(s) 159, 280, 497
HaeIII GGCC 1 cut(s) 169
HgaI GACGC 1 cut(s) 781
HhaI GCGC 2 cut(s) 234, 759
Hin1II CATG 4 cut(s) 193, 358, 770, 839
Hin6I GCGC 2 cut(s) 232, 757
HinP1I GCGC 2 cut(s) 232, 757
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 4 cut(s) 59, 392, 678, 872
HphI GGTGA 2 cut(s) 545, 703
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 2 cut(s) 58, 613
Hpy188III TCNNGA 3 cut(s) 246, 545, 876
Hpy8I GTNNAC 1 cut(s) 328
HpyAV CCTTC 3 cut(s) 496, 626, 750
HpyCH4III ACNGT 4 cut(s) 256, 338, 493, 821
HpyCH4IV ACGT 3 cut(s) 639, 778, 810
HpyCH4V TGCA 4 cut(s) 218, 242, 441, 496
HpySE526I ACGT 3 cut(s) 639, 778, 810
Hsp92II CATG 4 cut(s) 193, 358, 770, 839
HspAI GCGC 2 cut(s) 232, 757
KpnI GGTACC 1 cut(s) 825
Kzo9I GATC 2 cut(s) 285, 517
LmnI GCTCC 1 cut(s) 596
Lsp1109I GCAGC 3 cut(s) 145, 291, 508
LweI GCATC 1 cut(s) 251
MaeII ACGT 3 cut(s) 639, 778, 810
MaeIII GTNAC 2 cut(s) 72, 533
MalI GATC 2 cut(s) 287, 519
MboI GATC 2 cut(s) 285, 517
MluCI AATT 7 cut(s) 97, 235, 320, 442, 651, 707, 829
MlyI GAGTC 3 cut(s) 53, 687, 866
MnlI CCTC 7 cut(s) 286, 421, 586, 619, 637, 688, 846
Mph1103I ATGCAT 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 4 cut(s) 69, 86, 528, 827
MslI CAYNNNNRTG 2 cut(s) 491, 668
MspA1I CMGCKG 1 cut(s) 499
MspR9I CCNGG 3 cut(s) 522, 687, 850
MvaI CCWGG 3 cut(s) 522, 687, 850
NdeII GATC 2 cut(s) 285, 517
NlaIII CATG 4 cut(s) 193, 358, 770, 839
NlaIV GGNNCC 3 cut(s) 208, 340, 823
NmuCI GTSAC 2 cut(s) 72, 533
NsbI TGCGCA 1 cut(s) 233
NsiI ATGCAT 1 cut(s) 244
NspI RCATGY 1 cut(s) 839
OliI CACNNNNGTG 1 cut(s) 668
PciI ACATGT 1 cut(s) 835
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 392
PflMI CCANNNNNTGG 1 cut(s) 855
PfoI TCCNGGA 1 cut(s) 520
PkrI GCNGC 3 cut(s) 160, 281, 498
PleI GAGTC 3 cut(s) 53, 686, 866
PpsI GAGTC 3 cut(s) 53, 686, 866
Ppu21I YACGTR 1 cut(s) 811
PscI ACATGT 1 cut(s) 835
PsiI TTATAA 1 cut(s) 650
Psp6I CCWGG 3 cut(s) 520, 685, 848
PspGI CCWGG 3 cut(s) 520, 685, 848
PspN4I GGNNCC 3 cut(s) 208, 340, 823
PspPI GGNCC 5 cut(s) 103, 207, 222, 338, 358
PvuII CAGCTG 1 cut(s) 499
RsaI GTAC 2 cut(s) 813, 823
RsaNI GTAC 2 cut(s) 812, 822
RseI CAYNNNNRTG 2 cut(s) 491, 668
SaqAI TTAA 4 cut(s) 69, 86, 528, 827
SatI GCNGC 3 cut(s) 159, 280, 497
Sau3AI GATC 2 cut(s) 285, 517
Sau96I GGNCC 5 cut(s) 103, 207, 222, 338, 358
SchI GAGTC 3 cut(s) 53, 687, 866
ScrFI CCNGG 3 cut(s) 522, 687, 850
SfaNI GCATC 1 cut(s) 251
SinI GGWCC 5 cut(s) 103, 207, 222, 338, 358
SmiMI CAYNNNNRTG 2 cut(s) 491, 668
SmlI CTYRAG 1 cut(s) 543
SmoI CTYRAG 1 cut(s) 543
Sse9I AATT 7 cut(s) 97, 235, 320, 442, 651, 707, 829
SsiI CCGC 2 cut(s) 210, 698
StyD4I CCNGG 3 cut(s) 520, 685, 848
TaaI ACNGT 4 cut(s) 256, 338, 493, 821
TaiI ACGT 3 cut(s) 642, 781, 813
TaqI TCGA 4 cut(s) 92, 271, 395, 471
TasI AATT 7 cut(s) 97, 235, 320, 442, 651, 707, 829
TfiI GAWTC 1 cut(s) 392
Tru1I TTAA 4 cut(s) 69, 86, 528, 827
Tru9I TTAA 4 cut(s) 69, 86, 528, 827
TscAI CASTG 2 cut(s) 259, 498
TseFI GTSAC 2 cut(s) 72, 533
TseI GCWGC 3 cut(s) 158, 279, 496
Tsp45I GTSAC 2 cut(s) 72, 533
TspDTI ATGAA 5 cut(s) 119, 206, 343, 387, 405
TspGWI ACGGA 6 cut(s) 569, 574, 598, 680, 782, 787
TspRI CASTG 2 cut(s) 259, 498
Van91I CCANNNNNTGG 1 cut(s) 855
VpaK11BI GGWCC 5 cut(s) 103, 207, 222, 338, 358
XagI CCTNNNNNAGG 1 cut(s) 201
XapI RAATTY 3 cut(s) 97, 707, 829
XceI RCATGY 1 cut(s) 839
XmiI GTMKAC 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.