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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 117, 140 |
| AgsI | TTSAA | 4 cut(s) | 74, 104, 112, 128 |
| Alw26I | GTCTC | 3 cut(s) | 2, 45, 159 |
| ApoI | RAATTY | 2 cut(s) | 117, 140 |
| BauI | CACGAG | 1 cut(s) | 162 |
| BcoDI | GTCTC | 3 cut(s) | 2, 45, 159 |
| BsaI | GGTCTC | 1 cut(s) | 45 |
| BsmAI | GTCTC | 3 cut(s) | 2, 45, 159 |
| Bso31I | GGTCTC | 1 cut(s) | 45 |
| BspTNI | GGTCTC | 1 cut(s) | 45 |
| BssSI | CACGAG | 1 cut(s) | 162 |
| Bst2BI | CACGAG | 1 cut(s) | 162 |
| BstMAI | GTCTC | 3 cut(s) | 2, 45, 159 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 89 |
| Eco31I | GGTCTC | 1 cut(s) | 45 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 39, 71 |
| FspEI | CC | 6 cut(s) | 9, 33, 46, 51, 68, 135 |
| HpyAV | CCTTC | 1 cut(s) | 41 |
| HpyCH4V | TGCA | 1 cut(s) | 21 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 89 |
| LpnPI | CCDG | 1 cut(s) | 51 |
| MboII | GAAGA | 1 cut(s) | 124 |
| MluCI | AATT | 3 cut(s) | 69, 117, 140 |
| MmeI | TCCRAC | 1 cut(s) | 41 |
| MseI | TTAA | 1 cut(s) | 150 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 89 |
| SaqAI | TTAA | 1 cut(s) | 150 |
| SetI | ASST | 2 cut(s) | 40, 57 |
| SgeI | CNNG | 3 cut(s) | 30, 50, 137 |
| Sse9I | AATT | 3 cut(s) | 69, 117, 140 |
| SspI | AATATT | 1 cut(s) | 108 |
| TasI | AATT | 3 cut(s) | 69, 117, 140 |
| Tru1I | TTAA | 1 cut(s) | 150 |
| Tru9I | TTAA | 1 cut(s) | 150 |
| XapI | RAATTY | 2 cut(s) | 117, 140 |