Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000285

Physical Location & Seq

Genomic Coordinates Forward (+)

10506889 .. 10509960

Gene Structure 3072 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29561

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGGCTAGCATCAAAACCAGCTTTCGACTTCTTTTGGCTGTTTTCCTTCTTTTGTTAACTGATTCTGTTCCCTCGAGAGCCACTTTAGCCAACTCAGGTGCTAAAAATTTAACTTTGGCTTGCTTTGAGGAGGACCGGAAAGCCCTTCTTGAATTCAAACGCAGCCTTGATGATCCTTTACACCTACTTTCATCTTGGATAGGGGAAGACTGCTGCAAGTGGACGCGCGTACGCTGCAGCAACCAAACAGGCCATGTTGTCGAGCTTGACCTTAGCAACGTCTGTGGAGCAACTGGTGCTTCCAGGGAGGAACGTTCATGCTTAGGTGGTGAGCTATCTCCTTCTTTAGTTAATCTAACACACTTGAACTACTTGAACTTGAGTGGCAACACTTTCCATGGTATTCCCATCCCAAGTTTCATAGGTTCACTCGAGAAGTTGAGGTACCTTGACCTCTCCCTCTCGGGTTTTGTGGGAATGGTCCCTCCTCATCTCGGAAACCTATCGAAATTGCTCCATCTTGATCTCCATCTTGATCTCTCAACATATTCAGATATTTGGGTTTCAGATTTAAAGTGGCTCTCTGGTCTCTCTTCTTTGCAATACCTGGACTTGGACCGGTTGAACCTTAGCCAGGCCACAGATCATTGGGTTCATGCTATTAATATGCTTCCTTCACTCTTTGAGTTGCACTTTTCTTCTTGTAAACTTCATGGCAGCCTCCCACAGTCTCTTCCACATGTAAATTTGACGTCACTTTTGGTCATTGACCTCTCATCCAACAACTTTCAATCTTCATTACCTCAATGGCTTTTTAATATTAGTACTCTTGTGACGGCAGAGTTTGATTTTTCTGAATTTACTGGCTCCATTCCGGAAGTTTCATTGCTTAAGGACTTGGAAACTCTGGAACTGTCAAGGAACTCACTTTCCGGTCCATTTCCAATATCAATAGGAAATCTGTTACATTTGCAGGCCTTGCACCTTTCAGAAAACCCCATCTCTGGTTCAGTTCCTACATCAATAGGAAATCTATTACAGTTGAGGACCCTATCCCTTTCACAAAACTCAATCTCTGGTCCACTTCCGGCATCAATAGGAAATCTTTTCCATTTGGAAACCTTGGACCTTTCACAAAACTCAATCTCCGGTCCACTTCCAGCATCAATAGGAAATCTTTTCCATTTGGAAACCTTGGACCTCTCACAAAACTCAATCTCCGGTCCACTTCCTACGTCAGTAGGAAATCTGTCACATTTGACTATGCTAGACCTTTCCTTCAACATGATGAATGGCAGCATTCCAAAGAGTATTGGACAACTTACAGAATTAGCTTTCCTGAATCTGTTGTCTAATCAATGGGAAGGCGTCATAAGTGAAAGTCATTTGCAGAACCTCACAAGATTGCTTGACCTTCGATTGTCATCGAAGTCTCTGGTTTTCAACATCAGTCAGGAGTGGATTCCTTTCTTCAACTTATCTCTCATCACCATCCGTGATTGCCAATTTGTAAGTCCTGCATTTCCAGCATGGCTTAGAAGCCAATTCATCGTTTATATAGTTCTCTCAAATGTAGGTATTTCAGATACAATACCTGAGTGGTTTTGGAGAAACTCCCCACACCTGAGTTGGTTGGATCTCTCTAATAACCAATTAAGAGGAAAGCTTCCATACGTGGAGTTGGACCTCTCTGGTGTATATGTTAATTTGGAAAACAACTACTTGGAGGGTTCCATTCCACTTTGGCCAAATGTATTATACCTGAAGTTGGGAAGCAATAGATTTTCAGGACCAATTCCGTTGAATATTGGCCAGGAGATGTCAACACTAGGTAGCCTAGACCTTTCTAGGAATAATCTAAGCGGTAGCATTCCCCATTCACTAACTAAACTGAAGAGCTTGAGTTCTATTGATCTCTCGAGGAATTGTTTATCCGGAAGTATCCCTAAGGATTGGGAAGGACTGTTGACCATAGATTTTTCCAATAACAATCTATCTGGTCAAATTCCACCAAGCTTATGCTCTCAGCAAATTTCACCTTCCTGGTTGAGATTAAGTAATAACAATCTTTCTGGGGAGCTTGGACCGTCCTTGCAAAATTGTCAAAACTTGTTTACACTCGATCTTGGAGGGAACAAGTTCTCTGGAGCCATACCACAGTGGATTGGAGAAGACCTACGATCCTTGCAATTTTTACTTCTTGGAGAGAACAAGTTCACCGGAAGTATTCCTCAACAACTGTGCGCTCTCTCTGGTCTTCATGTTTTAGACCTATCTCAGAATAATTTATCAGGCTCGATCCCAAGATGTCTTGATAATTTGACACAATTGACAAATTTAGGTAGCATGGCTCAGCCATTTCCCTCTCTTAGGGACACGCCGGATATGGATCCATGGCATATGGATTTGGATCCGAAAGGCGTAGAATATGAATATACTAGTATTCTACCTCTTGTAAACACCATTGACCTGTCAAGCAATAACCTATCCGGAGAAATACCAGAAGAGATGAGAAACCTCTCCAACTTGGGTAGCTTGAATTTATCTAGGAATCTGTTGACAGGAAAGATACCGGAGGATATTGGAAGCTTACAACGGCTACAAACTCTTGACCTCTCCTGTAACCATCTTCATGGTCCAATTCCTCTAAGCATGACCGATATGACTTTGCTGAGCAAATTGAACTTGTCATATAACGACTTGTCTGGGCCAATCCCATCTGCTAACCAATTCCAAACCTTCAATGATCCAACTTTATTTGAAGGAAACTTGGGACTTTGTGGCATTCCATTGCCAACTCAGTGCTCATCATCCCCTGATGGGAATCCAGAAGTTAAAGAAGATGCAGGTGAAGATGAAGAAGGGTCTGGAAAGATATGGTTCTATGTAAGCACTACGATGGGATTCATTATGGGATTTTGGGCTGTTTTTGGCAGTTTAGTGCTAAAGAGGTCATGGAGACATGCCTATTTCAAGTTTGTCGACGACATGAAAGACAGGCTAATTCTGGTAATCACACTGAAATGCATGGCTTGTCTACGCAGAATGACTGAACAAGAAAGACAAACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0010051 GO:0010232 GO:0010233 GO:0010305 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048545 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

1023

Amino Acids

112.91

Weight (kDa)

5.15

Isoelectric Point (pI)

37.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	44 - 80	3.8e-11	Leucine rich repeat N-terminal domain
LRR_14	PF23598	115 - 305	7e-08	Leucine-rich repeat region
LRR_14	PF23598	292 - 377	2e-08	Leucine-rich repeat region
LRR_8	PF13855	300 - 358	3e-06	Leucine rich repeat
LRR_8	PF13855	347 - 406	1.1e-06	Leucine rich repeat
LRR_14	PF23598	365 - 553	1.5e-18	Leucine-rich repeat region
LRR_8	PF13855	395 - 453	3.5e-06	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47885
fragaria_vesca	FvH4_3g09550 FvH4_4g32560
malus_domestica	MD01G1172200.v1.1
rosa_chinensis	RchiOBHm_Chr1g0353911 RchiOBHm_Chr1g0354141 RchiOBHm_Chr1g0354151 RchiOBHm_Chr1g0354161 RchiOBHm_Chr1g0354191 RchiOBHm_Chr1g0354201 RchiOBHm_Chr1g0354221 RchiOBHm_Chr1g0354271 RchiOBHm_Chr1g0354301 RchiOBHm_Chr1g0354321 RchiOBHm_Chr1g0354371 RchiOBHm_Chr1g0354401 RchiOBHm_Chr1g0354411 RchiOBHm_Chr1g0354451 RchiOBHm_Chr1g0354551 RchiOBHm_Chr1g0359231 RchiOBHm_Chr3g0470501 RchiOBHm_Chr4g0440611 RchiOBHm_Chr4g0440631 RchiOBHm_Chr4g0441421 RchiOBHm_Chr4g0441431 RchiOBHm_Chr5g0015191 RchiOBHm_Chr5g0022441 RchiOBHm_Chr7g0207081
rosa_laevigata	RLG00000009355 RLG00000009356 RLG00000023813 RLG00000024262 RLG00000028199 RLG00000028200 RLG00000028204 RLG00000028209 RLG00000028211 RLG00000028214 RLG00000028216 RLG00000028220 RLG00000028221 RLG00000028223 RLG00000028224 RLG00000028886 RLG00000029706 RLG00000032176
rosa_multiflora	Rmu_co8366321.1_g000001 Rmu_sc0001926.1_g000008 Rmu_sc0001926.1_g000009 Rmu_sc0002115.1_g000006 Rmu_sc0002115.1_g000007 Rmu_sc0002209.1_g000020 Rmu_sc0002737.1_g000005 Rmu_sc0002737.1_g000009 Rmu_sc0002737.1_g000015 Rmu_sc0002737.1_g000016 Rmu_sc0002737.1_g000018 Rmu_sc0002930.1_g000003 Rmu_sc0002930.1_g000005 Rmu_sc0003292.1_g000004 Rmu_sc0003360.1_g000004 Rmu_sc0003360.1_g000005 Rmu_sc0003381.1_g000013 Rmu_sc0004299.1_g000001 Rmu_sc0007159.1_g000001 Rmu_sc0007159.1_g000007 Rmu_sc0009777.1_g000002 Rmu_sc0010755.1_g000002 Rmu_sc0012353.1_g000001 Rmu_sc0012562.1_g000003 Rmu_sc0012562.1_g000004 Rmu_sc0012562.1_g000006 Rmu_sc0012562.1_g000013 Rmu_sc0012562.1_g000021 Rmu_sc0015525.1_g000001 Rmu_sc0015525.1_g000005 Rmu_sc0018378.1_g000001 Rmu_sc0022127.1_g000002 Rmu_sc0022144.1_g000002 Rmu_sc0035791.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056680 Rroxscaffold_1G00061360 Rroxscaffold_3G00236900 Rroxscaffold_4G00300940 Rroxscaffold_4G00301040 Rroxscaffold_4G00301050 Rroxscaffold_4G00301070 Rroxscaffold_4G00301100 Rroxscaffold_4G00301110 Rroxscaffold_4G00301930 Rroxscaffold_4G00301940 Rroxscaffold_4G00301950 Rroxscaffold_7G00202690
rosa_rugosa	Rorug01G0242000 Rorug01G0242100 Rorug01G0242200 Rorug01G0242300 Rorug01G0242700 Rorug01G0242900 Rorug01G0243000 Rorug03G0109600 Rorug03G0109700 Rorug04G0327000 Rorug04G0333500 Rorug04G0333600 Rorug05G0024600 RorugPtG0003000
rosa_samantha	Rh1AG253200 Rh1AG253400 Rh1BG225200 Rh1CG177100 Rh1CG234500 Rh1CG237000 Rh1DG252000 Rh1DG252400 Rh1DG252900 Rh3CG085900 Rh4BG399300 Rh4DG391900
rosa_wichuraiana	Rw1G015760 Rw1G022030 Rw1G022100 Rw1G022120 Rw1G022230 Rw5G010290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2837
AatII	GACGTC	1 cut(s)	755
Acc36I	ACCTGC	1 cut(s)	2837
Acc65I	GGTACC	1 cut(s)	444
AccB1I	GGYRCC	1 cut(s)	444
AccI	GTMKAC	2 cut(s)	2982, 3037
AccII	CGCG	2 cut(s)	226, 228
AccIII	TCCGGA	3 cut(s)	874, 1934, 2489
AciI	CCGC	1 cut(s)	1863
AclI	AACGTT	1 cut(s)	313
AclWI	GGATC	9 cut(s)	167, 1644, 2175, 2293, 2384, 2397, 2405, 2418, 2741
AcoI	YGGCCR	2 cut(s)	1745, 1810
AcsI	RAATTY	8 cut(s)	106, 152, 745, 857, 2004, 2031, 2335, 2539
AcuI	CTGAAG	2 cut(s)	1784, 1913
AcyI	GRCGYC	2 cut(s)	752, 1368
AfaI	GTAC	3 cut(s)	231, 446, 826
AfiI	CCNNNNNNNGG	7 cut(s)	494, 613, 634, 1004, 1768, 2370, 2820
AflII	CTTAAG	1 cut(s)	890
AflIII	ACRYGT	1 cut(s)	739
AgeI	ACCGGT	1 cut(s)	618
AhlI	ACTAGT	1 cut(s)	2438
AjnI	CCWGG	5 cut(s)	302, 606, 633, 1812, 2042
AloI	GAACNNNNNNTCC	2 cut(s)	914, 946
Alw21I	GWGCWC	1 cut(s)	2807
Alw26I	GTCTC	4 cut(s)	593, 735, 1437, 2953
AlwI	GGATC	9 cut(s)	167, 1644, 2175, 2293, 2384, 2397, 2405, 2418, 2741
Ama87I	CYCGRG	4 cut(s)	73, 431, 463, 1918
Aor13HI	TCCGGA	3 cut(s)	874, 1934, 2489
AoxI	GGCC	6 cut(s)	250, 636, 975, 1745, 1810, 2708
ApeKI	GCWGC	6 cut(s)	162, 213, 234, 237, 717, 1296
ApoI	RAATTY	8 cut(s)	106, 152, 745, 857, 2004, 2031, 2335, 2539
ArsI	GACNNNNNNTTYG	2 cut(s)	742, 774
AseI	ATTAAT	1 cut(s)	663
AsiGI	ACCGGT	1 cut(s)	618
Asp700I	GAANNNNTTC	2 cut(s)	2138, 2213
Asp718I	GGTACC	1 cut(s)	444
AspLEI	GCGC	2 cut(s)	228, 2246
AsuHPI	GGTGA	5 cut(s)	341, 1480, 2028, 2209, 2861
AsuNHI	GCTAGC	1 cut(s)	5
AvaI	CYCGRG	4 cut(s)	73, 431, 463, 1918
AxyI	CCTNAGG	1 cut(s)	1947
BalI	TGGCCA	2 cut(s)	1747, 1812
BamHI	GGATCC	2 cut(s)	2389, 2410
BanI	GGYRCC	1 cut(s)	444
BarI	GAAGNNNNNNTAC	2 cut(s)	428, 460
BbsI	GAAGAC	3 cut(s)	213, 2178, 2249
Bbv12I	GWGCWC	1 cut(s)	2807
BbvI	GCAGC	6 cut(s)	174, 200, 221, 249, 729, 1308
BccI	CCATC	9 cut(s)	416, 525, 537, 1007, 1499, 2634, 2725, 2813, 2893
BceAI	ACGGC	2 cut(s)	852, 2612
BciT130I	CCWGG	5 cut(s)	304, 608, 635, 1814, 2044
BciVI	GTATCC	1 cut(s)	1952
BcoDI	GTCTC	4 cut(s)	593, 735, 1437, 2953
BcuI	ACTAGT	1 cut(s)	2438
BfaI	CTAG	7 cut(s)	6, 1268, 1829, 1838, 1848, 2439, 2547
BfmI	CTRYAG	1 cut(s)	235
BfrI	CTTAAG	1 cut(s)	890
BfuAI	ACCTGC	1 cut(s)	2837
BfuI	GTATCC	1 cut(s)	1952
BisI	GCNGC	6 cut(s)	163, 214, 235, 238, 718, 1297
BlpI	GCTNAGC	2 cut(s)	2352, 2672
BlsI	GCNGC	6 cut(s)	164, 215, 236, 239, 719, 1298
BmcAI	AGTACT	1 cut(s)	826
Bme1390I	CCNGG	5 cut(s)	304, 608, 635, 1814, 2044
BmeT110I	CYCGRG	4 cut(s)	73, 431, 463, 1918
BmiI	GGNNCC	8 cut(s)	446, 483, 868, 1049, 1732, 2149, 2391, 2412
BmrFI	CCNGG	5 cut(s)	304, 608, 635, 1814, 2044
BmsI	GCATC	4 cut(s)	18, 1100, 1172, 2833
BmtI	GCTAGC	1 cut(s)	9
BpiI	GAAGAC	3 cut(s)	213, 2178, 2249
BpmI	CTGGAG	1 cut(s)	2166
Bpu10I	CCTNAGC	3 cut(s)	272, 322, 629
Bpu1102I	GCTNAGC	2 cut(s)	2352, 2672
BpuEI	CTTGAG	2 cut(s)	400, 1921
BsaAI	YACGTR	1 cut(s)	1675
BsaBI	GATNNNNATC	5 cut(s)	528, 1423, 2388, 2409, 2823
BsaHI	GRCGYC	2 cut(s)	752, 1368
BsaI	GGTCTC	1 cut(s)	593
BsaJI	CCNNGG	5 cut(s)	303, 397, 1122, 1194, 2393
BsaXI	ACNNNNNCTCC	4 cut(s)	1130, 1160, 1202, 1232
Bsc4I	CCNNNNNNNGG	7 cut(s)	494, 613, 634, 1004, 1768, 2370, 2820
Bse118I	RCCGGY	1 cut(s)	618
Bse1I	ACTGG	2 cut(s)	298, 868
Bse21I	CCTNAGG	1 cut(s)	1947
Bse3DI	GCAATG	2 cut(s)	884, 2789
Bse8I	GATNNNNATC	5 cut(s)	528, 1423, 2388, 2409, 2823
BseAI	TCCGGA	3 cut(s)	874, 1934, 2489
BseBI	CCWGG	5 cut(s)	304, 608, 635, 1814, 2044
BseDI	CCNNGG	5 cut(s)	303, 397, 1122, 1194, 2393
BseGI	GGATG	4 cut(s)	408, 776, 1491, 2810
BseJI	GATNNNNATC	5 cut(s)	528, 1423, 2388, 2409, 2823
BseLI	CCNNNNNNNGG	7 cut(s)	494, 613, 634, 1004, 1768, 2370, 2820
BseMI	GCAATG	2 cut(s)	884, 2789
BseMII	CTCAG	8 cut(s)	108, 1587, 1616, 2039, 2291, 2366, 2663, 2813
BseNI	ACTGG	2 cut(s)	298, 868
BseRI	GAGGAG	2 cut(s)	143, 477
BseXI	GCAGC	6 cut(s)	174, 200, 221, 249, 729, 1308
Bsh1236I	CGCG	2 cut(s)	226, 228
BshFI	GGCC	6 cut(s)	252, 638, 977, 1747, 1812, 2710
BshNI	GGYRCC	1 cut(s)	444
BshTI	ACCGGT	1 cut(s)	618
BsiHKAI	GWGCWC	1 cut(s)	2807
BsiHKCI	CYCGRG	4 cut(s)	73, 431, 463, 1918
BsiWI	CGTACG	1 cut(s)	229
BslFI	GGGAC	3 cut(s)	467, 2387, 2787
BslI	CCNNNNNNNGG	7 cut(s)	494, 613, 634, 1004, 1768, 2370, 2820
BsmAI	GTCTC	4 cut(s)	593, 735, 1437, 2953
BsmFI	GGGAC	3 cut(s)	467, 2387, 2787
BsmI	GAATGC	3 cut(s)	1299, 1869, 2784
BsnI	GGCC	6 cut(s)	252, 638, 977, 1747, 1812, 2710
Bso31I	GGTCTC	1 cut(s)	593
BsoBI	CYCGRG	4 cut(s)	73, 431, 463, 1918
Bsp1286I	GDGCHC	1 cut(s)	2807
Bsp13I	TCCGGA	3 cut(s)	874, 1934, 2489
Bsp1720I	GCTNAGC	2 cut(s)	2352, 2672
Bsp19I	CCATGG	2 cut(s)	397, 2393
BspACI	CCGC	1 cut(s)	1863
BspANI	GGCC	6 cut(s)	252, 638, 977, 1747, 1812, 2710
BspCNI	CTCAG	8 cut(s)	107, 1588, 1617, 2038, 2290, 2365, 2664, 2812
BspEI	TCCGGA	3 cut(s)	874, 1934, 2489
BspFNI	CGCG	2 cut(s)	226, 228
BspLI	GGNNCC	8 cut(s)	446, 483, 868, 1049, 1732, 2149, 2391, 2412
BspMAI	CTGCAG	1 cut(s)	239
BspMI	ACCTGC	1 cut(s)	2837
BspOI	GCTAGC	1 cut(s)	9
BspPI	GGATC	9 cut(s)	167, 1644, 2175, 2293, 2384, 2397, 2405, 2418, 2741
BspQI	GCTCTTC	1 cut(s)	1889
BspT107I	GGYRCC	1 cut(s)	444
BspTI	CTTAAG	1 cut(s)	890
BspTNI	GGTCTC	1 cut(s)	593
BsrDI	GCAATG	2 cut(s)	884, 2789
BsrFI	RCCGGY	1 cut(s)	618
BsrI	ACTGG	2 cut(s)	298, 868
BssAI	RCCGGY	1 cut(s)	618
BssECI	CCNNGG	5 cut(s)	303, 397, 1122, 1194, 2393
BssNI	GRCGYC	2 cut(s)	752, 1368
BssT1I	CCWWGG	4 cut(s)	397, 1122, 1194, 2393
Bst2UI	CCWGG	5 cut(s)	304, 608, 635, 1814, 2044
Bst4CI	ACNGT	7 cut(s)	729, 915, 1041, 1965, 2088, 2160, 2241
Bst6I	CTCTTC	4 cut(s)	598, 738, 1889, 2499
BstACI	GRCGYC	2 cut(s)	752, 1368
BstAFI	CTTAAG	1 cut(s)	890
BstAPI	GCANNNNNTGC	2 cut(s)	296, 979
BstBAI	YACGTR	1 cut(s)	1675
BstC8I	GCNNGC	3 cut(s)	7, 121, 975
BstDSI	CCRYGG	2 cut(s)	397, 2393
BstENI	CCTNNNNNAGG	2 cut(s)	632, 2368
BstF5I	GGATG	4 cut(s)	408, 776, 1491, 2810
BstFNI	CGCG	2 cut(s)	226, 228
BstHHI	GCGC	2 cut(s)	228, 2246
BstMAI	GTCTC	4 cut(s)	593, 735, 1437, 2953
BstMWI	GCNNNNNNNGC	5 cut(s)	86, 234, 296, 979, 1526
BstNI	CCWGG	5 cut(s)	304, 608, 635, 1814, 2044
BstNSI	RCATGY	2 cut(s)	743, 2966
BstSCI	CCNGG	5 cut(s)	302, 606, 633, 1812, 2042
BstSFI	CTRYAG	1 cut(s)	235
BstUI	CGCG	2 cut(s)	226, 228
BstV1I	GCAGC	6 cut(s)	174, 200, 221, 249, 729, 1308
BstV2I	GAAGAC	3 cut(s)	213, 2178, 2249
BstX2I	RGATCY	3 cut(s)	1636, 2389, 2410
BstXI	CCANNNNNNTGG	1 cut(s)	2633
BstYI	RGATCY	3 cut(s)	1636, 2389, 2410
Bsu36I	CCTNAGG	1 cut(s)	1947
BsuI	GTATCC	1 cut(s)	1952
BsuRI	GGCC	6 cut(s)	252, 638, 977, 1747, 1812, 2710
BtgI	CCRYGG	2 cut(s)	397, 2393
BtsCI	GGATG	4 cut(s)	408, 776, 1491, 2810
BtsIMutI	CAGTG	3 cut(s)	2165, 2807, 3017
BveI	ACCTGC	1 cut(s)	2837
Cac8I	GCNNGC	3 cut(s)	7, 121, 975
CfoI	GCGC	2 cut(s)	228, 2246
Cfr10I	RCCGGY	1 cut(s)	618
CseI	GACGC	2 cut(s)	232, 1357
Csp6I	GTAC	3 cut(s)	230, 445, 825
CspAI	ACCGGT	1 cut(s)	618
CspCI	CAANNNNNGTGG	2 cut(s)	265, 300
CviQI	GTAC	3 cut(s)	230, 445, 825
DraI	TTTAAA	1 cut(s)	573
EaeI	YGGCCR	2 cut(s)	1745, 1810
Eam1104I	CTCTTC	4 cut(s)	598, 738, 1889, 2499
EarI	CTCTTC	4 cut(s)	598, 738, 1889, 2499
Eco130I	CCWWGG	4 cut(s)	397, 1122, 1194, 2393
Eco147I	AGGCCT	1 cut(s)	977
Eco31I	GGTCTC	1 cut(s)	593
Eco57I	CTGAAG	2 cut(s)	1784, 1913
Eco81I	CCTNAGG	1 cut(s)	1947
Eco88I	CYCGRG	4 cut(s)	73, 431, 463, 1918
EcoNI	CCTNNNNNAGG	2 cut(s)	632, 2368
EcoO109I	RGGNCCY	1 cut(s)	1047
EcoRI	GAATTC	1 cut(s)	152
EcoRII	CCWGG	5 cut(s)	302, 606, 633, 1812, 2042
EcoT14I	CCWWGG	4 cut(s)	397, 1122, 1194, 2393
EcoT22I	ATGCAT	1 cut(s)	3029
ErhI	CCWWGG	4 cut(s)	397, 1122, 1194, 2393
FalI	AAGNNNNNCTT	2 cut(s)	132, 164
FaqI	GGGAC	3 cut(s)	467, 2387, 2787
FauNDI	CATATG	1 cut(s)	2400
FblI	GTMKAC	2 cut(s)	2982, 3037
Fnu4HI	GCNGC	6 cut(s)	163, 214, 235, 238, 718, 1297
FokI	GGATG	4 cut(s)	395, 763, 1478, 2797
Fsp4HI	GCNGC	6 cut(s)	163, 214, 235, 238, 718, 1297
FspBI	CTAG	7 cut(s)	6, 1268, 1829, 1838, 1848, 2439, 2547
GlaI	GCGC	2 cut(s)	227, 2245
GluI	GCNGC	6 cut(s)	163, 214, 235, 238, 718, 1297
GsuI	CTGGAG	1 cut(s)	2166
HaeIII	GGCC	6 cut(s)	252, 638, 977, 1747, 1812, 2710
HgaI	GACGC	2 cut(s)	232, 1357
HhaI	GCGC	2 cut(s)	228, 2246
Hin1I	GRCGYC	2 cut(s)	752, 1368
Hin6I	GCGC	2 cut(s)	226, 2244
HinP1I	GCGC	2 cut(s)	226, 2244
HincII	GTYRAC	5 cut(s)	57, 1824, 1968, 2559, 2983
HindII	GTYRAC	5 cut(s)	57, 1824, 1968, 2559, 2983
HindIII	AAGCTT	3 cut(s)	1664, 2014, 2587
HinfI	GANTC	6 cut(s)	62, 1342, 1462, 2551, 2824, 2904
HpaI	GTTAAC	1 cut(s)	57
HphI	GGTGA	5 cut(s)	341, 1480, 2028, 2209, 2861
Hpy188I	TCNGA	8 cut(s)	497, 553, 568, 856, 991, 1585, 2280, 2415
Hpy99I	CGWCG	1 cut(s)	2987
HpyCH4III	ACNGT	7 cut(s)	729, 915, 1041, 1965, 2088, 2160, 2241
HpyCH4IV	ACGT	6 cut(s)	279, 313, 752, 1235, 1674, 3068
HpyF10VI	GCNNNNNNNGC	5 cut(s)	86, 234, 296, 979, 1526
HpySE526I	ACGT	6 cut(s)	279, 313, 752, 1235, 1674, 3068
Hsp92I	GRCGYC	2 cut(s)	752, 1368
HspAI	GCGC	2 cut(s)	226, 2244
Kpn2I	TCCGGA	3 cut(s)	874, 1934, 2489
KpnI	GGTACC	1 cut(s)	448
KspAI	GTTAAC	1 cut(s)	57
LguI	GCTCTTC	1 cut(s)	1889
LmnI	GCTCC	5 cut(s)	287, 519, 872, 2077, 2147
Lsp1109I	GCAGC	6 cut(s)	174, 200, 221, 249, 729, 1308
LweI	GCATC	4 cut(s)	18, 1100, 1172, 2833
MaeI	CTAG	7 cut(s)	6, 1268, 1829, 1838, 1848, 2439, 2547
MaeII	ACGT	6 cut(s)	279, 313, 752, 1235, 1674, 3068
MaeIII	GTNAC	5 cut(s)	753, 832, 963, 1251, 2621
MfeI	CAATTG	1 cut(s)	2327
MflI	RGATCY	3 cut(s)	1636, 2389, 2410
MhlI	GDGCHC	1 cut(s)	2807
MlsI	TGGCCA	2 cut(s)	1747, 1812
MluNI	TGGCCA	2 cut(s)	1747, 1812
MmeI	TCCRAC	5 cut(s)	804, 1614, 1662, 2547, 2774
Mox20I	TGGCCA	2 cut(s)	1747, 1812
Mph1103I	ATGCAT	1 cut(s)	3029
MroI	TCCGGA	3 cut(s)	874, 1934, 2489
MroXI	GAANNNNTTC	2 cut(s)	2138, 2213
MscI	TGGCCA	2 cut(s)	1747, 1812
MslI	CAYNNNNRTG	3 cut(s)	2631, 2897, 3022
Msp20I	TGGCCA	2 cut(s)	1747, 1812
MspCI	CTTAAG	1 cut(s)	890
MspR9I	CCNGG	5 cut(s)	304, 608, 635, 1814, 2044
MunI	CAATTG	1 cut(s)	2327
Mva1269I	GAATGC	3 cut(s)	1299, 1869, 2784
MvaI	CCWGG	5 cut(s)	304, 608, 635, 1814, 2044
MvnI	CGCG	2 cut(s)	226, 228
MwoI	GCNNNNNNNGC	5 cut(s)	86, 234, 296, 979, 1526
NcoI	CCATGG	2 cut(s)	397, 2393
NdeI	CATATG	1 cut(s)	2400
NheI	GCTAGC	1 cut(s)	5
NlaIV	GGNNCC	8 cut(s)	446, 483, 868, 1049, 1732, 2149, 2391, 2412
NmuCI	GTSAC	3 cut(s)	753, 832, 1251
NsiI	ATGCAT	1 cut(s)	3029
NspI	RCATGY	2 cut(s)	743, 2966
PaeR7I	CTCGAG	3 cut(s)	73, 431, 1918
PaqCI	CACCTGC	1 cut(s)	2837
PceI	AGGCCT	1 cut(s)	977
PciI	ACATGT	1 cut(s)	739
PciSI	GCTCTTC	1 cut(s)	1889
PctI	GAATGC	3 cut(s)	1299, 1869, 2784
PdmI	GAANNNNTTC	2 cut(s)	2138, 2213
PfeI	GAWTC	6 cut(s)	62, 1342, 1462, 2551, 2824, 2904
Pfl23II	CGTACG	1 cut(s)	229
PinAI	ACCGGT	1 cut(s)	618
PkrI	GCNGC	6 cut(s)	164, 215, 236, 239, 719, 1298
Ppu21I	YACGTR	1 cut(s)	1675
PpuMI	RGGWCCY	1 cut(s)	1047
PscI	ACATGT	1 cut(s)	739
PshBI	ATTAAT	1 cut(s)	663
Psp1406I	AACGTT	1 cut(s)	313
Psp5II	RGGWCCY	1 cut(s)	1047
Psp6I	CCWGG	5 cut(s)	302, 606, 633, 1812, 2042
PspGI	CCWGG	5 cut(s)	302, 606, 633, 1812, 2042
PspLI	CGTACG	1 cut(s)	229
PspN4I	GGNNCC	8 cut(s)	446, 483, 868, 1049, 1732, 2149, 2391, 2412
PspPPI	RGGWCCY	1 cut(s)	1047
PstI	CTGCAG	1 cut(s)	239
PsuI	RGATCY	3 cut(s)	1636, 2389, 2410
RsaI	GTAC	3 cut(s)	231, 446, 826
RsaNI	GTAC	3 cut(s)	230, 445, 825
RseI	CAYNNNNRTG	3 cut(s)	2631, 2897, 3022
SalI	GTCGAC	1 cut(s)	2981
SapI	GCTCTTC	1 cut(s)	1889
SatI	GCNGC	6 cut(s)	163, 214, 235, 238, 718, 1297
ScaI	AGTACT	1 cut(s)	826
ScrFI	CCNGG	5 cut(s)	304, 608, 635, 1814, 2044
SduI	GDGCHC	1 cut(s)	2807
SfaNI	GCATC	4 cut(s)	18, 1100, 1172, 2833
SfcI	CTRYAG	1 cut(s)	235
Sfr274I	CTCGAG	3 cut(s)	73, 431, 1918
SlaI	CTCGAG	3 cut(s)	73, 431, 1918
SmiMI	CAYNNNNRTG	3 cut(s)	2631, 2897, 3022
SmlI	CTYRAG	6 cut(s)	73, 379, 431, 890, 1900, 1918
SmoI	CTYRAG	6 cut(s)	73, 379, 431, 890, 1900, 1918
SpeI	ACTAGT	1 cut(s)	2438
SseBI	AGGCCT	1 cut(s)	977
SsiI	CCGC	1 cut(s)	1863
SspI	AATATT	2 cut(s)	820, 1807
SspMI	CTAG	7 cut(s)	6, 1268, 1829, 1838, 1848, 2439, 2547
StuI	AGGCCT	1 cut(s)	977
StyD4I	CCNGG	5 cut(s)	302, 606, 633, 1812, 2042
StyI	CCWWGG	4 cut(s)	397, 1122, 1194, 2393
TaaI	ACNGT	7 cut(s)	729, 915, 1041, 1965, 2088, 2160, 2241
TaiI	ACGT	6 cut(s)	282, 316, 755, 1238, 1677, 3071
TaqII	GACCGA	1 cut(s)	2672
TatI	WGTACW	1 cut(s)	824
TfiI	GAWTC	6 cut(s)	62, 1342, 1462, 2551, 2824, 2904
TscAI	CASTG	3 cut(s)	2165, 2807, 3024
TseFI	GTSAC	3 cut(s)	753, 832, 1251
TseI	GCWGC	6 cut(s)	162, 213, 234, 237, 717, 1296
Tsp45I	GTSAC	3 cut(s)	753, 832, 1251
TspGWI	ACGGA	2 cut(s)	1484, 1788
TspRI	CASTG	3 cut(s)	2165, 2807, 3024
Vha464I	CTTAAG	1 cut(s)	890
VspI	ATTAAT	1 cut(s)	663
XagI	CCTNNNNNAGG	2 cut(s)	632, 2368
XapI	RAATTY	8 cut(s)	106, 152, 745, 857, 2004, 2031, 2335, 2539
XceI	RCATGY	2 cut(s)	743, 2966
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1626
XhoI	CTCGAG	3 cut(s)	73, 431, 1918
XmiI	GTMKAC	2 cut(s)	2982, 3037
XmnI	GAANNNNTTC	2 cut(s)	2138, 2213
XspI	CTAG	7 cut(s)	6, 1268, 1829, 1838, 1848, 2439, 2547
ZraI	GACGTC	1 cut(s)	753
ZrmI	AGTACT	1 cut(s)	826
Zsp2I	ATGCAT	1 cut(s)	3029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.