Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0000285

Physical Location & Seq

Genomic Coordinates Forward (+)

11822293 .. 11825447

Gene Structure 3155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032176

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1890 bp

ATGGCTAGCATCAAAACCAGCTTTCGACTTCTTTTGGCTGTTTTCCTTCTTTTGTTAACTGATTTTGTTCCCTCGAGAGCCACTTTAGCCAACTCAGGTGCTAAAAATTTACCTTTGGCTTGCTTTGAGGAGGACCGGAAAGCCCTTCTTGAATTCAAACGCAGCCTTGATGATCCTTTACACCTACTTTCATCTTGGATAGGGGAAGACTGCTGCAAGTGGACGCGCGTACGCTGCAGCAACCAAACAGGCCATGTTGTCGAGCTTGACCTTAGCAACGACTGTGGAGCAACTGGTGCTTCCAGGGAGGAACGGTCATGCTTAGGTGGTGAGCTATCTCCTTCTTTAGTTAATCTAACACACTTGAACTACTTGAACTTGAGTGGCAACACTTTCCATGGTATTCCCATCCCAAGTTTCATAGGTTCACTCGAGAAGTTGAGCCTCCCACAGTCTCTTCCACATGTAAATTTGACGTCACTTTTGGTCATTGACCTCTCATTCAACAACTTTCAATCTTCATTACCTCAATGGCTTTTTAATATTAGTACTCTTGTGACGGTAGAGTTTTATTTTTCTGAATTTACTGGCTCCATTCCGGAAGTTTCATTGCTTAAGGACTTGGAAACTCTGGAACTGTCAAGGAACTCACTTTCCGGTCCATTTCCAACATCAATAGGAAATCTGTTACATTTGCAGGCCCTGCACCTTTCAGAAAACCCCATCTCTGGCTCAGTTCCTACATCAATAGGAAATCTATTACAGTTGAGGACCCTATACCTTTCACAAAACTCAATCTCTGGTCCACTTCCGGCATCAATAGGAAATCTTTTCCATTTGGAAACCTTGGACCTTTCACAAAACTCAATCTCCGGTCCACTTCCAGCATCAATAGGAAATCTTTTCCATTTGGATACCTTGGACCTCTCAAAAAACAAAATATCTGGTCCACTTCCTATGTCAGTAGGAAATCTGTCACATTTGACTGTGCTAGACCTTTCCTTCAACATGATGAATGGCAGCATTCCAAAGAGTATTGGACAACTTACAGAATTAGCTACCCTGAATCTGTTGTCTAATCAATGGGAAGGCGTCATAAGTGAAAGTCATTTGCAGAACCTCACAAGCTTGGCTGACCTTCAATTGTCATCGAAGTCTCTGGTTTTCAACATCAGTCAGGAGTGGATTCCTTTCTTCAACGCATCTTTCATCACTATCCGTGATTGCCAATTTGTAAGTCCTGCATTTCCAGCATGGCTTAGAAGCCAATACATCGTTTATATAGTTCTCTCAAATGTAGGTAGCATGGCTCAGCCATTTCCCTCTCTTAAGGACACGCCGGATATGGATCCATGGCATATGGATTTGGATCCGAAAGGCGTAGAATATGAATATACTAGTATTCTACCTCTTGTAAACACCATTGACCTGTCAAGCAATAATCTATCCGGAGAAATACCAGAAGAGATGAGAAACCTCTCCAACTTGGGTAGCTTGAATTTATCTCGGAATCTGTTGACAGGAAAGATACCGGAGGATATTGGAAGCTTACAACGGTTACAAACTCTTGACCTCTCCTGTAACCATCTTCATGGTCCAATTCCTCTAAGCATGACCGATATGACTTTGCTGAGCAAATTGAACTTGTCATATAACGACTTGTCTGGGCCAATCCCATCTGCTAACCAATTCCAAACCTTCAATGATCCAACTTTATTTGAAGGAAACTTGGGACTTTGTGGCGCTCCATTGCCAACTCAGTGCTCATCATCCCCTGATGGGAATCCAGAAGTTAAAGAAGATGCAGGTGAAGATGAAGAAGGGTCTGGAAAGATATGGTTCTATGATTTCAACTTGGATTTGTTTTCTGTTGTATTTCATGTATACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0010051 GO:0010232 GO:0010233 GO:0010305 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048545 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

630

Amino Acids

68.97

Weight (kDa)

4.72

Isoelectric Point (pI)

38.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	44 - 80	2.1e-11	Leucine rich repeat N-terminal domain
LRR_8	PF13855	208 - 266	4.6e-08	Leucine rich repeat
LRR_14	PF23598	249 - 424	5.8e-23	Leucine-rich repeat region
LRR_14	PF23598	481 - 550	1e-05	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0000285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47885
fragaria_vesca	FvH4_3g09550 FvH4_4g32560
malus_domestica	MD01G1172200.v1.1
rosa_chinensis	RchiOBHm_Chr1g0353911 RchiOBHm_Chr1g0354141 RchiOBHm_Chr1g0354151 RchiOBHm_Chr1g0354161 RchiOBHm_Chr1g0354191 RchiOBHm_Chr1g0354201 RchiOBHm_Chr1g0354221 RchiOBHm_Chr1g0354271 RchiOBHm_Chr1g0354301 RchiOBHm_Chr1g0354321 RchiOBHm_Chr1g0354371 RchiOBHm_Chr1g0354401 RchiOBHm_Chr1g0354411 RchiOBHm_Chr1g0354451 RchiOBHm_Chr1g0354551 RchiOBHm_Chr1g0359231 RchiOBHm_Chr3g0470501 RchiOBHm_Chr4g0440611 RchiOBHm_Chr4g0440631 RchiOBHm_Chr4g0441421 RchiOBHm_Chr4g0441431 RchiOBHm_Chr5g0015191 RchiOBHm_Chr5g0022441 RchiOBHm_Chr7g0207081
rosa_laevigata	RLG00000009355 RLG00000009356 RLG00000023813 RLG00000024262 RLG00000028199 RLG00000028200 RLG00000028204 RLG00000028209 RLG00000028211 RLG00000028214 RLG00000028216 RLG00000028220 RLG00000028221 RLG00000028223 RLG00000028224 RLG00000028886 RLG00000029706 RLG00000032176
rosa_multiflora	Rmu_co8366321.1_g000001 Rmu_sc0001926.1_g000008 Rmu_sc0001926.1_g000009 Rmu_sc0002115.1_g000006 Rmu_sc0002115.1_g000007 Rmu_sc0002209.1_g000020 Rmu_sc0002737.1_g000005 Rmu_sc0002737.1_g000009 Rmu_sc0002737.1_g000015 Rmu_sc0002737.1_g000016 Rmu_sc0002737.1_g000018 Rmu_sc0002930.1_g000003 Rmu_sc0002930.1_g000005 Rmu_sc0003292.1_g000004 Rmu_sc0003360.1_g000004 Rmu_sc0003360.1_g000005 Rmu_sc0003381.1_g000013 Rmu_sc0004299.1_g000001 Rmu_sc0007159.1_g000001 Rmu_sc0007159.1_g000007 Rmu_sc0009777.1_g000002 Rmu_sc0010755.1_g000002 Rmu_sc0012353.1_g000001 Rmu_sc0012562.1_g000003 Rmu_sc0012562.1_g000004 Rmu_sc0012562.1_g000006 Rmu_sc0012562.1_g000013 Rmu_sc0012562.1_g000021 Rmu_sc0015525.1_g000001 Rmu_sc0015525.1_g000005 Rmu_sc0018378.1_g000001 Rmu_sc0022127.1_g000002 Rmu_sc0022144.1_g000002 Rmu_sc0035791.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056680 Rroxscaffold_1G00061360 Rroxscaffold_3G00236900 Rroxscaffold_4G00300940 Rroxscaffold_4G00301040 Rroxscaffold_4G00301050 Rroxscaffold_4G00301070 Rroxscaffold_4G00301100 Rroxscaffold_4G00301110 Rroxscaffold_4G00301930 Rroxscaffold_4G00301940 Rroxscaffold_4G00301950 Rroxscaffold_7G00202690
rosa_rugosa	Rorug01G0242000 Rorug01G0242100 Rorug01G0242200 Rorug01G0242300 Rorug01G0242700 Rorug01G0242900 Rorug01G0243000 Rorug03G0109600 Rorug03G0109700 Rorug04G0327000 Rorug04G0333500 Rorug04G0333600 Rorug05G0024600 RorugPtG0003000
rosa_samantha	Rh1AG253200 Rh1AG253400 Rh1BG225200 Rh1CG177100 Rh1CG234500 Rh1CG237000 Rh1DG252000 Rh1DG252400 Rh1DG252900 Rh3CG085900 Rh4BG399300 Rh4DG391900
rosa_wichuraiana	Rw1G015760 Rw1G022030 Rw1G022100 Rw1G022120 Rw1G022230 Rw5G010290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1796
AatII	GACGTC	1 cut(s)	479
Acc36I	ACCTGC	1 cut(s)	1796
AccI	GTMKAC	1 cut(s)	1884
AccII	CGCG	2 cut(s)	226, 228
AccIII	TCCGGA	2 cut(s)	598, 1448
AclWI	GGATC	6 cut(s)	167, 1343, 1356, 1364, 1377, 1700
AcsI	RAATTY	5 cut(s)	106, 152, 469, 581, 1498
AcyI	GRCGYC	2 cut(s)	476, 1092
AfaI	GTAC	2 cut(s)	231, 550
AfiI	CCNNNNNNNGG	2 cut(s)	728, 1779
AflII	CTTAAG	2 cut(s)	614, 1328
AflIII	ACRYGT	1 cut(s)	463
AhlI	ACTAGT	1 cut(s)	1397
AjnI	CCWGG	1 cut(s)	302
AloI	GAACNNNNNNTCC	2 cut(s)	638, 670
AluBI	AGCT	7 cut(s)	21, 265, 334, 1058, 1128, 1494, 1548
AluI	AGCT	7 cut(s)	21, 265, 334, 1058, 1128, 1494, 1548
Alw21I	GWGCWC	1 cut(s)	1766
Alw26I	GTCTC	2 cut(s)	459, 1161
AlwI	GGATC	6 cut(s)	167, 1343, 1356, 1364, 1377, 1700
AlwNI	CAGNNNCTG	1 cut(s)	703
Ama87I	CYCGRG	2 cut(s)	73, 431
Aor13HI	TCCGGA	2 cut(s)	598, 1448
AoxI	GGCC	3 cut(s)	250, 699, 1667
ApeKI	GCWGC	5 cut(s)	162, 213, 234, 237, 1020
ApoI	RAATTY	5 cut(s)	106, 152, 469, 581, 1498
ArsI	GACNNNNNNTTYG	2 cut(s)	466, 498
AspLEI	GCGC	2 cut(s)	228, 1745
AsuHPI	GGTGA	2 cut(s)	341, 1820
AsuNHI	GCTAGC	1 cut(s)	5
AvaI	CYCGRG	2 cut(s)	73, 431
AvaII	GGWCC	9 cut(s)	133, 659, 771, 803, 850, 875, 922, 947, 1595
BamHI	GGATCC	2 cut(s)	1348, 1369
BbsI	GAAGAC	1 cut(s)	213
Bbv12I	GWGCWC	1 cut(s)	1766
BbvI	GCAGC	5 cut(s)	174, 200, 221, 249, 1032
BccI	CCATC	5 cut(s)	416, 731, 1593, 1684, 1772
BciT130I	CCWGG	1 cut(s)	304
BciVI	GTATCC	1 cut(s)	907
BcoDI	GTCTC	2 cut(s)	459, 1161
BcuI	ACTAGT	1 cut(s)	1397
BfaI	CTAG	4 cut(s)	6, 992, 1398, 1888
BfmI	CTRYAG	1 cut(s)	235
BfoI	RGCGCY	1 cut(s)	1746
BfrI	CTTAAG	2 cut(s)	614, 1328
BfuAI	ACCTGC	1 cut(s)	1796
BfuI	GTATCC	1 cut(s)	907
BisI	GCNGC	5 cut(s)	163, 214, 235, 238, 1021
BlpI	GCTNAGC	2 cut(s)	1311, 1631
BlsI	GCNGC	5 cut(s)	164, 215, 236, 239, 1022
BmcAI	AGTACT	1 cut(s)	550
Bme1390I	CCNGG	1 cut(s)	304
Bme18I	GGWCC	9 cut(s)	133, 659, 771, 803, 850, 875, 922, 947, 1595
BmeT110I	CYCGRG	2 cut(s)	73, 431
BmiI	GGNNCC	4 cut(s)	592, 773, 1350, 1371
BmrFI	CCNGG	1 cut(s)	304
BmsI	GCATC	5 cut(s)	18, 824, 896, 1211, 1792
BmtI	GCTAGC	1 cut(s)	9
BpiI	GAAGAC	1 cut(s)	213
Bpu10I	CCTNAGC	2 cut(s)	272, 322
Bpu1102I	GCTNAGC	2 cut(s)	1311, 1631
BpuEI	CTTGAG	1 cut(s)	400
BsaBI	GATNNNNATC	3 cut(s)	1347, 1368, 1782
BsaHI	GRCGYC	2 cut(s)	476, 1092
BsaJI	CCNNGG	5 cut(s)	303, 397, 846, 918, 1352
BsaWI	WCCGGW	6 cut(s)	135, 598, 656, 872, 1448, 1531
BsaXI	ACNNNNNCTCC	4 cut(s)	299, 329, 854, 884
Bsc4I	CCNNNNNNNGG	2 cut(s)	728, 1779
Bse1I	ACTGG	2 cut(s)	298, 592
Bse3DI	GCAATG	2 cut(s)	608, 1748
Bse8I	GATNNNNATC	3 cut(s)	1347, 1368, 1782
BseAI	TCCGGA	2 cut(s)	598, 1448
BseBI	CCWGG	1 cut(s)	304
BseDI	CCNNGG	5 cut(s)	303, 397, 846, 918, 1352
BseGI	GGATG	2 cut(s)	408, 1769
BseJI	GATNNNNATC	3 cut(s)	1347, 1368, 1782
BseLI	CCNNNNNNNGG	2 cut(s)	728, 1779
BseMI	GCAATG	2 cut(s)	608, 1748
BseMII	CTCAG	5 cut(s)	108, 747, 1325, 1622, 1772
BseNI	ACTGG	2 cut(s)	298, 592
BseRI	GAGGAG	1 cut(s)	143
BseXI	GCAGC	5 cut(s)	174, 200, 221, 249, 1032
BsgI	GTGCAG	1 cut(s)	689
Bsh1236I	CGCG	2 cut(s)	226, 228
BshFI	GGCC	3 cut(s)	252, 701, 1669
BsiHKAI	GWGCWC	1 cut(s)	1766
BsiHKCI	CYCGRG	2 cut(s)	73, 431
BsiSI	CCGG	8 cut(s)	136, 599, 657, 812, 873, 1340, 1449, 1532
BsiWI	CGTACG	1 cut(s)	229
BslFI	GGGAC	1 cut(s)	1746
BslI	CCNNNNNNNGG	2 cut(s)	728, 1779
BsmAI	GTCTC	2 cut(s)	459, 1161
BsmFI	GGGAC	1 cut(s)	1746
BsmI	GAATGC	1 cut(s)	1023
BsnI	GGCC	3 cut(s)	252, 701, 1669
BsoBI	CYCGRG	2 cut(s)	73, 431
Bsp1286I	GDGCHC	1 cut(s)	1766
Bsp13I	TCCGGA	2 cut(s)	598, 1448
Bsp143I	GATC	4 cut(s)	172, 1348, 1369, 1705
Bsp1720I	GCTNAGC	2 cut(s)	1311, 1631
Bsp19I	CCATGG	2 cut(s)	397, 1352
BspANI	GGCC	3 cut(s)	252, 701, 1669
BspCNI	CTCAG	5 cut(s)	107, 746, 1324, 1623, 1771
BspEI	TCCGGA	2 cut(s)	598, 1448
BspFNI	CGCG	2 cut(s)	226, 228
BspLI	GGNNCC	4 cut(s)	592, 773, 1350, 1371
BspMAI	CTGCAG	1 cut(s)	239
BspMI	ACCTGC	1 cut(s)	1796
BspOI	GCTAGC	1 cut(s)	9
BspPI	GGATC	6 cut(s)	167, 1343, 1356, 1364, 1377, 1700
BspTI	CTTAAG	2 cut(s)	614, 1328
BsrDI	GCAATG	2 cut(s)	608, 1748
BsrI	ACTGG	2 cut(s)	298, 592
BssECI	CCNNGG	5 cut(s)	303, 397, 846, 918, 1352
BssMI	GATC	4 cut(s)	172, 1348, 1369, 1705
BssNAI	GTATAC	1 cut(s)	1885
BssNI	GRCGYC	2 cut(s)	476, 1092
BssT1I	CCWWGG	4 cut(s)	397, 846, 918, 1352
Bst1107I	GTATAC	1 cut(s)	1885
Bst2UI	CCWGG	1 cut(s)	304
Bst4CI	ACNGT	8 cut(s)	284, 315, 453, 562, 639, 765, 988, 1557
Bst6I	CTCTTC	2 cut(s)	462, 1458
BstACI	GRCGYC	2 cut(s)	476, 1092
BstAFI	CTTAAG	2 cut(s)	614, 1328
BstAPI	GCANNNNNTGC	2 cut(s)	296, 703
BstC8I	GCNNGC	3 cut(s)	7, 121, 699
BstDEI	CTNAG	9 cut(s)	94, 272, 322, 733, 1259, 1311, 1607, 1631, 1758
BstDSI	CCRYGG	2 cut(s)	397, 1352
BstF5I	GGATG	2 cut(s)	408, 1769
BstFNI	CGCG	2 cut(s)	226, 228
BstH2I	RGCGCY	1 cut(s)	1746
BstHHI	GCGC	2 cut(s)	228, 1745
BstKTI	GATC	4 cut(s)	175, 1351, 1372, 1708
BstMAI	GTCTC	2 cut(s)	459, 1161
BstMBI	GATC	4 cut(s)	172, 1348, 1369, 1705
BstMWI	GCNNNNNNNGC	5 cut(s)	86, 234, 296, 703, 1250
BstNI	CCWGG	1 cut(s)	304
BstNSI	RCATGY	1 cut(s)	467
BstSCI	CCNGG	1 cut(s)	302
BstSFI	CTRYAG	1 cut(s)	235
BstUI	CGCG	2 cut(s)	226, 228
BstV1I	GCAGC	5 cut(s)	174, 200, 221, 249, 1032
BstV2I	GAAGAC	1 cut(s)	213
BstX2I	RGATCY	2 cut(s)	1348, 1369
BstXI	CCANNNNNNTGG	1 cut(s)	1592
BstYI	RGATCY	2 cut(s)	1348, 1369
BstZ17I	GTATAC	1 cut(s)	1885
BsuI	GTATCC	1 cut(s)	907
BsuRI	GGCC	3 cut(s)	252, 701, 1669
BtgI	CCRYGG	2 cut(s)	397, 1352
BtsCI	GGATG	2 cut(s)	408, 1769
BtsIMutI	CAGTG	1 cut(s)	1766
BveI	ACCTGC	1 cut(s)	1796
Cac8I	GCNNGC	3 cut(s)	7, 121, 699
CaiI	CAGNNNCTG	1 cut(s)	703
CfoI	GCGC	2 cut(s)	228, 1745
CseI	GACGC	2 cut(s)	232, 1081
Csp6I	GTAC	2 cut(s)	230, 549
CspCI	CAANNNNNGTGG	2 cut(s)	265, 300
CviQI	GTAC	2 cut(s)	230, 549
DdeI	CTNAG	9 cut(s)	94, 272, 322, 733, 1259, 1311, 1607, 1631, 1758
DpnI	GATC	4 cut(s)	174, 1350, 1371, 1707
DpnII	GATC	4 cut(s)	172, 1348, 1369, 1705
Eam1104I	CTCTTC	2 cut(s)	462, 1458
EarI	CTCTTC	2 cut(s)	462, 1458
Eco130I	CCWWGG	4 cut(s)	397, 846, 918, 1352
Eco47I	GGWCC	9 cut(s)	133, 659, 771, 803, 850, 875, 922, 947, 1595
Eco88I	CYCGRG	2 cut(s)	73, 431
EcoO109I	RGGNCCY	2 cut(s)	700, 771
EcoRI	GAATTC	1 cut(s)	152
EcoRII	CCWGG	1 cut(s)	302
EcoT14I	CCWWGG	4 cut(s)	397, 846, 918, 1352
ErhI	CCWWGG	4 cut(s)	397, 846, 918, 1352
FalI	AAGNNNNNCTT	2 cut(s)	132, 164
FaqI	GGGAC	1 cut(s)	1746
FauNDI	CATATG	1 cut(s)	1359
FblI	GTMKAC	1 cut(s)	1884
Fnu4HI	GCNGC	5 cut(s)	163, 214, 235, 238, 1021
FokI	GGATG	2 cut(s)	395, 1756
Fsp4HI	GCNGC	5 cut(s)	163, 214, 235, 238, 1021
FspBI	CTAG	4 cut(s)	6, 992, 1398, 1888
GlaI	GCGC	2 cut(s)	227, 1744
GluI	GCNGC	5 cut(s)	163, 214, 235, 238, 1021
HaeII	RGCGCY	1 cut(s)	1746
HaeIII	GGCC	3 cut(s)	252, 701, 1669
HapII	CCGG	8 cut(s)	136, 599, 657, 812, 873, 1340, 1449, 1532
HgaI	GACGC	2 cut(s)	232, 1081
HhaI	GCGC	2 cut(s)	228, 1745
Hin1I	GRCGYC	2 cut(s)	476, 1092
Hin6I	GCGC	2 cut(s)	226, 1743
HinP1I	GCGC	2 cut(s)	226, 1743
HincII	GTYRAC	2 cut(s)	57, 1518
HindII	GTYRAC	2 cut(s)	57, 1518
HindIII	AAGCTT	2 cut(s)	1126, 1546
HinfI	GANTC	4 cut(s)	1066, 1186, 1510, 1783
HpaI	GTTAAC	1 cut(s)	57
HpaII	CCGG	8 cut(s)	136, 599, 657, 812, 873, 1340, 1449, 1532
HphI	GGTGA	2 cut(s)	341, 1820
Hpy166II	GTNNAC	9 cut(s)	57, 222, 428, 806, 878, 950, 1417, 1518, 1885
Hpy188I	TCNGA	4 cut(s)	580, 715, 1374, 1509
Hpy8I	GTNNAC	9 cut(s)	57, 222, 428, 806, 878, 950, 1417, 1518, 1885
HpyAV	CCTTC	9 cut(s)	56, 155, 351, 1012, 1082, 1148, 1708, 1715, 1814
HpyCH4III	ACNGT	8 cut(s)	284, 315, 453, 562, 639, 765, 988, 1557
HpyCH4IV	ACGT	1 cut(s)	476
HpyCH4V	TGCA	7 cut(s)	216, 237, 697, 706, 1114, 1244, 1805
HpyF10VI	GCNNNNNNNGC	5 cut(s)	86, 234, 296, 703, 1250
HpyF3I	CTNAG	9 cut(s)	94, 272, 322, 733, 1259, 1311, 1607, 1631, 1758
HpySE526I	ACGT	1 cut(s)	476
Hsp92I	GRCGYC	2 cut(s)	476, 1092
HspAI	GCGC	2 cut(s)	226, 1743
Kpn2I	TCCGGA	2 cut(s)	598, 1448
KspAI	GTTAAC	1 cut(s)	57
Kzo9I	GATC	4 cut(s)	172, 1348, 1369, 1705
LmnI	GCTCC	3 cut(s)	287, 596, 1750
Lsp1109I	GCAGC	5 cut(s)	174, 200, 221, 249, 1032
LweI	GCATC	5 cut(s)	18, 824, 896, 1211, 1792
MaeI	CTAG	4 cut(s)	6, 992, 1398, 1888
MaeII	ACGT	1 cut(s)	476
MaeIII	GTNAC	6 cut(s)	477, 556, 687, 975, 1557, 1580
MalI	GATC	4 cut(s)	174, 1350, 1371, 1707
MboI	GATC	4 cut(s)	172, 1348, 1369, 1705
MboII	GAAGA	9 cut(s)	218, 449, 510, 1186, 1475, 1580, 1811, 1823, 1829
MfeI	CAATTG	1 cut(s)	1142
MflI	RGATCY	2 cut(s)	1348, 1369
MhlI	GDGCHC	1 cut(s)	1766
MmeI	TCCRAC	3 cut(s)	692, 1506, 1733
MroI	TCCGGA	2 cut(s)	598, 1448
MseI	TTAA	6 cut(s)	56, 351, 540, 615, 1329, 1794
MslI	CAYNNNNRTG	1 cut(s)	1590
MspCI	CTTAAG	2 cut(s)	614, 1328
MspI	CCGG	8 cut(s)	136, 599, 657, 812, 873, 1340, 1449, 1532
MspR9I	CCNGG	1 cut(s)	304
MunI	CAATTG	1 cut(s)	1142
Mva1269I	GAATGC	1 cut(s)	1023
MvaI	CCWGG	1 cut(s)	304
MvnI	CGCG	2 cut(s)	226, 228
MwoI	GCNNNNNNNGC	5 cut(s)	86, 234, 296, 703, 1250
NcoI	CCATGG	2 cut(s)	397, 1352
NdeI	CATATG	1 cut(s)	1359
NdeII	GATC	4 cut(s)	172, 1348, 1369, 1705
NheI	GCTAGC	1 cut(s)	5
NlaIV	GGNNCC	4 cut(s)	592, 773, 1350, 1371
NmuCI	GTSAC	3 cut(s)	477, 556, 975
NspI	RCATGY	1 cut(s)	467
PaeR7I	CTCGAG	2 cut(s)	73, 431
PaqCI	CACCTGC	1 cut(s)	1796
PciI	ACATGT	1 cut(s)	463
PctI	GAATGC	1 cut(s)	1023
PfeI	GAWTC	4 cut(s)	1066, 1186, 1510, 1783
Pfl23II	CGTACG	1 cut(s)	229
PkrI	GCNGC	5 cut(s)	164, 215, 236, 239, 1022
PpuMI	RGGWCCY	1 cut(s)	771
PscI	ACATGT	1 cut(s)	463
Psp5II	RGGWCCY	1 cut(s)	771
Psp6I	CCWGG	1 cut(s)	302
PspGI	CCWGG	1 cut(s)	302
PspLI	CGTACG	1 cut(s)	229
PspN4I	GGNNCC	4 cut(s)	592, 773, 1350, 1371
PspPPI	RGGWCCY	1 cut(s)	771
PstI	CTGCAG	1 cut(s)	239
PstNI	CAGNNNCTG	1 cut(s)	703
PsuI	RGATCY	2 cut(s)	1348, 1369
RsaI	GTAC	2 cut(s)	231, 550
RsaNI	GTAC	2 cut(s)	230, 549
RseI	CAYNNNNRTG	1 cut(s)	1590
SaqAI	TTAA	6 cut(s)	56, 351, 540, 615, 1329, 1794
SatI	GCNGC	5 cut(s)	163, 214, 235, 238, 1021
Sau3AI	GATC	4 cut(s)	172, 1348, 1369, 1705
ScaI	AGTACT	1 cut(s)	550
ScrFI	CCNGG	1 cut(s)	304
SduI	GDGCHC	1 cut(s)	1766
SfaNI	GCATC	5 cut(s)	18, 824, 896, 1211, 1792
SfcI	CTRYAG	1 cut(s)	235
Sfr274I	CTCGAG	2 cut(s)	73, 431
SinI	GGWCC	9 cut(s)	133, 659, 771, 803, 850, 875, 922, 947, 1595
SlaI	CTCGAG	2 cut(s)	73, 431
SmiMI	CAYNNNNRTG	1 cut(s)	1590
SmlI	CTYRAG	5 cut(s)	73, 379, 431, 614, 1328
SmoI	CTYRAG	5 cut(s)	73, 379, 431, 614, 1328
SpeI	ACTAGT	1 cut(s)	1397
SspI	AATATT	1 cut(s)	544
SspMI	CTAG	4 cut(s)	6, 992, 1398, 1888
StyD4I	CCNGG	1 cut(s)	302
StyI	CCWWGG	4 cut(s)	397, 846, 918, 1352
TaaI	ACNGT	8 cut(s)	284, 315, 453, 562, 639, 765, 988, 1557
TaiI	ACGT	1 cut(s)	479
TaqI	TCGA	5 cut(s)	25, 74, 261, 432, 1151
TaqII	GACCGA	1 cut(s)	1631
TatI	WGTACW	1 cut(s)	548
TfiI	GAWTC	4 cut(s)	1066, 1186, 1510, 1783
Tru1I	TTAA	6 cut(s)	56, 351, 540, 615, 1329, 1794
Tru9I	TTAA	6 cut(s)	56, 351, 540, 615, 1329, 1794
TscAI	CASTG	1 cut(s)	1766
TseFI	GTSAC	3 cut(s)	477, 556, 975
TseI	GCWGC	5 cut(s)	162, 213, 234, 237, 1020
Tsp45I	GTSAC	3 cut(s)	477, 556, 975
TspGWI	ACGGA	1 cut(s)	1208
TspRI	CASTG	1 cut(s)	1766
Vha464I	CTTAAG	2 cut(s)	614, 1328
VpaK11BI	GGWCC	9 cut(s)	133, 659, 771, 803, 850, 875, 922, 947, 1595
XapI	RAATTY	5 cut(s)	106, 152, 469, 581, 1498
XceI	RCATGY	1 cut(s)	467
XhoI	CTCGAG	2 cut(s)	73, 431
XmiI	GTMKAC	1 cut(s)	1884
XspI	CTAG	4 cut(s)	6, 992, 1398, 1888
ZraI	GACGTC	1 cut(s)	477
ZrmI	AGTACT	1 cut(s)	550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.