Rroxscaffold_4G00301820

Enoyl-(Acyl carrier protein) reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000170
Physical Location & Seq
Reverse (-)
22147173 .. 22150732
3560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301820.1

Sequence Viewer

Length: 1368 bp
ATGGCAGAAGGAGCAAAGAGCTATGCAGTTGTTACAGGTTCAAATCAAGGGATTGGATTTGGAACAGTTACGCTATTGGCTTCAAAAGGGGTCAAGGTTGTGCTAACTGCTTTGGATGAGAAGAGAGGTCTTGAAGCTATTGAAAAGCTAAAAGACTATGGCTTCTCAGATCTTGTGGTTTATCATCAGCTCAATGTAACTGACCCTAATAGCATTTCTTTCCTTGCCAATTTTGTCAAAACCCAATTCGGGAAACTCAATATCTTGGATGATGCAGAAGTCAACTTGAGTAAAATAATCACTCAAACATATGAGTTAGCTGAAGAATGTGTGAAAATAAACTACTATGGTGCCAAGAAAATCACTGAAGCATTCCTTCCTCTCCTTCTGCTATCTGAGTCTCCAAGACTTTCCAATATTACTTCTGGCGTCAGACAGTTAACAGTTAACGGTATGAAGTATGTTAACACCTATAGTTATTTAATTTTAATATTCAGAAAACTACAAATGTACTCATTTTCTGCATGTTGTTTTGATCAGCATATACCAAATGAATGGGCCAGAGGGTTGTTGAGTGATGCTGAGAATCTTACAGAAGAGCGAGTGGATGAGGTTTTGAGGGACTTTCTAAAAGACTTCAAAGAAGATATGCTGGAAACCAAAGGCTGGCCTACTACTCTTTCTGCATATATACTCTCCAAAGCAGCCTTGAATGCATACACTAGAATTGTGGCCAAGAAGTACACAAACTTCGGCGTCAATTGTGTCTGCCCTGGATTTGTCAAATCAGAAATGAGCTTCAATGCTGGTGTCTTAACCCCTGATGAAGCTGCTGAAATTATTGTGCGGTTAACAGTGTTTCCGAATGGCAATCCTTCTGGACTCTTCTTCTCTTCGGAAGGGCTTGGATTCGGACTAGTTAGGCAATTGGCTTCAAATGGGGTCATAACTGTGCTAACTGCCAGAGATGAAAAGAAGGGTATTGAAGCTGTTCAAAAATTAAAAGAGTTTGGTCTCTCAAACCTTGTGGCTTTTCATCCACTTGATGTTACAGACCCTACTAGCTTTACCTCCTTTGCAGACTATGTCAAATGCTGTTTTGGGAAACTGGATATCTTGAACTACTTAATGCGTGAATTTCCACTTGAGCAGTACTATTATGATCTAGAGACAGTAGTAATCCCAAATTATCCTCTTTTACATGTAAACAATGCAGGGGTTGGTGGAACCATTAAAGACCGTGAAGCTTCCATAGCTGCTGCAGCTGGTATGGTTAAGCTTATACCAAATGAGTGGGCTAAAGGGGTGTTAATTGATGCTGAGAAACTCACAGAAGGAGAAGAGAAGAAGTTTTGGAAGAATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

455

Amino Acids

50.53

Weight (kDa)

5.44

Isoelectric Point (pI)

20.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 9 - 149 8.5e-16 short chain dehydrogenase
adh_short_C2 PF13561 15 - 149 9.8e-12 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 299 - 373 6.3e-07 short chain dehydrogenase
adh_short_C2 PF13561 299 - 373 5.2e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01800 AT1G01800 AT2G24190 AT2G24190 AT3G61220 AT3G61220 AT3G61220
fragaria_vesca FvH4_7g13591 FvH4_7g13601 FvH4_7g13602 FvH4_7g13603 FvH4_7g13603 FvH4_7g13603 FvH4_7g13620
malus_domestica MD01G1052500.v1.1 MD01G1052600.v1.1 MD01G1052700.v1.1 MD01G1052800.v1.1 MD01G1053000.v1.1 MD01G1053100.v1.1 MD01G1053200.v1.1 MD01G1053300.v1.1 MD04G1099300.v1.1 MD04G1099400.v1.1 MD04G1099500.v1.1 MD07G1064800.v1.1 MD07G1142200.v1.1 MD07G1142300.v1.1
prunus_persica Prupe.1G171500_v2.0.a1 Prupe.1G171600_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160400_v2.0.a1 Prupe.2G160500_v2.0.a1 Prupe.2G160600_v2.0.a1 Prupe.2G160700_v2.0.a1 Prupe.2G160700_v2.0.a1 Prupe.2G160800_v2.0.a1 Prupe.2G160800_v2.0.a1 Prupe.2G160900_v2.0.a1 Prupe.2G161000_v2.0.a1 Prupe.2G161100_v2.0.a1 Prupe.2G161100_v2.0.a1 Prupe.2G161200_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161400_v2.0.a1 Prupe.2G161500_v2.0.a1 Prupe.2G161600_v2.0.a1 Prupe.2G161600_v2.0.a1 Prupe.2G161800_v2.0.a1 Prupe.2G161900_v2.0.a1 Prupe.2G161900_v2.0.a1 Prupe.2G162200_v2.0.a1 Prupe.2G162300_v2.0.a1 Prupe.2G162300_v2.0.a1 Prupe.4G240700_v2.0.a1 Prupe.4G240800_v2.0.a1
pyrus_communis pycom01g07820 pycom01g07840 pycom01g07850 pycom01g07860 pycom01g07880 pycom01g07900 pycom04g09450 pycom04g09460 pycom07g01290 pycom07g12490 pycom07g12510 pycom07g12570 pycom07g14080
rosa_chinensis RchiOBHm_Chr1g0353391 RchiOBHm_Chr1g0353401 RchiOBHm_Chr1g0353411 RchiOBHm_Chr1g0353421 RchiOBHm_Chr1g0353431 RchiOBHm_Chr1g0353481 RchiOBHm_Chr1g0353511 RchiOBHm_Chr1g0353531 RchiOBHm_Chr1g0353541 RchiOBHm_Chr1g0353571 RchiOBHm_Chr1g0353581 RchiOBHm_Chr1g0353621 RchiOBHm_Chr1g0353661 RchiOBHm_Chr7g0224711 RchiOBHm_Chr7g0224731 RchiOBHm_Chr7g0224751
rosa_laevigata RLG00000001859 RLG00000001863 RLG00000028244 RLG00000028267 RLG00000028269 RLG00000028270 RLG00000028272
rosa_multiflora Rmu_co8260681.1_g000001 Rmu_co8305447.1_g000001 Rmu_co8330951.1_g000001 Rmu_co8341853.1_g000001 Rmu_sc0000144.1_g000019 Rmu_sc0000144.1_g000020 Rmu_sc0000144.1_g000026 Rmu_sc0000687.1_g000012 Rmu_sc0000687.1_g000018 Rmu_sc0000687.1_g000019 Rmu_sc0000687.1_g000020 Rmu_sc0000687.1_g000026 Rmu_sc0001207.1_g000001 Rmu_sc0001207.1_g000012 Rmu_sc0005914.1_g000002 Rmu_sc0005914.1_g000005 Rmu_sc0005914.1_g000007 Rmu_sc0005947.1_g000025 Rmu_sc0007658.1_g000005 Rmu_sc0011028.1_g000012 Rmu_sc0011028.1_g000013 Rmu_sc0026118.1_g000001 Rmu_sc0026975.1_g000001 Rmu_sc0027167.1_g000001 Rmu_sc0042792.1_g000001
rosa_roxburghii Rroxscaffold_3G00234470 Rroxscaffold_4G00301760 Rroxscaffold_4G00301810 Rroxscaffold_4G00301820 Rroxscaffold_4G00301830 Rroxscaffold_4G00301840 Rroxscaffold_4G00301860 Rroxscaffold_4G00301870
rosa_rugosa Rorug01G0235700 Rorug01G0236300 Rorug01G0236600 Rorug01G0236800 Rorug07G0225000.1 Rorug07G0225100
rosa_samantha Rh1AG247900 Rh1AG248000 Rh1AG248400 Rh1AG248500 Rh1AG248600 Rh1AG248800 Rh1AG249000 Rh1AG249100 Rh1BG217700 Rh1BG218000 Rh1BG218400 Rh1BG218600 Rh1BG218800 Rh1BG218900 Rh1BG219200 Rh1DG244000 Rh1DG244300 Rh1DG244800 Rh1DG245000 Rh1DG245200 Rh1DG245300 Rh1DG245600 Rh1DG246200 Rh7BG361100 Rh7CG396500 Rh7CG396700 Rh7DG371200
rosa_wichuraiana Rw1G021600 Rw1G021610 Rw1G021620 Rw1G021640 Rw1G021650 Rw1G021660 Rw1G021680 Rw1G021710 Rw1G021900 Rw7G031330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 350
AccB7I CCANNNNNTGG 1 cut(s) 666
AciI CCGC 1 cut(s) 847
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 2 cut(s) 1136, 1360
AcuI CTGAAG 2 cut(s) 342, 387
AcyI GRCGYC 2 cut(s) 429, 756
AfaI GTAC 3 cut(s) 512, 743, 1154
AfiI CCNNNNNNNGG 2 cut(s) 249, 666
AflIII ACRYGT 1 cut(s) 1201
AhlI ACTAGT 1 cut(s) 916
AjnI CCWGG 1 cut(s) 772
Alw26I GTCTC 3 cut(s) 405, 1019, 1163
AoxI GGCC 3 cut(s) 558, 668, 732
ApeKI GCWGC 5 cut(s) 704, 830, 1256, 1259, 1262
ApoI RAATTY 2 cut(s) 1136, 1360
Asp700I GAANNNNTTC 2 cut(s) 990, 1361
AspS9I GGNCC 1 cut(s) 558
BalI TGGCCA 1 cut(s) 734
BanI GGYRCC 1 cut(s) 350
BarI GAAGNNNNNNTAC 2 cut(s) 734, 766
BbvI GCAGC 5 cut(s) 716, 817, 1243, 1246, 1274
BciT130I CCWGG 1 cut(s) 774
BclI TGATCA 1 cut(s) 535
BcoDI GTCTC 3 cut(s) 405, 1019, 1163
BcuI ACTAGT 1 cut(s) 916
BfaI CTAG 4 cut(s) 723, 917, 1062, 1166
BfmI CTRYAG 2 cut(s) 472, 1260
BglII AGATCT 1 cut(s) 169
BisI GCNGC 5 cut(s) 705, 831, 1257, 1260, 1263
BlsI GCNGC 5 cut(s) 706, 832, 1258, 1261, 1264
BmcAI AGTACT 1 cut(s) 1154
Bme1390I CCNGG 1 cut(s) 774
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 2 cut(s) 352, 1228
BmrFI CCNGG 1 cut(s) 774
BmsI GCATC 3 cut(s) 262, 568, 1306
BpuEI CTTGAG 2 cut(s) 307, 1166
BsaHI GRCGYC 2 cut(s) 429, 756
BsaI GGTCTC 1 cut(s) 1019
BsaJI CCNNGG 1 cut(s) 772
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 666
Bse1I ACTGG 1 cut(s) 1113
BseBI CCWGG 1 cut(s) 774
BseDI CCNNGG 1 cut(s) 772
BseGI GGATG 4 cut(s) 121, 274, 613, 1036
BseLI CCNNNNNNNGG 2 cut(s) 249, 666
BseMII CTCAG 4 cut(s) 180, 387, 573, 1311
BseNI ACTGG 1 cut(s) 1113
BseXI GCAGC 5 cut(s) 716, 817, 1243, 1246, 1274
BshFI GGCC 3 cut(s) 560, 670, 734
BshNI GGYRCC 1 cut(s) 350
BslFI GGGAC 1 cut(s) 635
BslI CCNNNNNNNGG 2 cut(s) 249, 666
BsmAI GTCTC 3 cut(s) 405, 1019, 1163
BsmFI GGGAC 1 cut(s) 635
BsmI GAATGC 2 cut(s) 371, 718
BsnI GGCC 3 cut(s) 560, 670, 734
Bso31I GGTCTC 1 cut(s) 1019
Bsp143I GATC 3 cut(s) 169, 535, 1162
BspACI CCGC 1 cut(s) 847
BspANI GGCC 3 cut(s) 560, 670, 734
BspCNI CTCAG 4 cut(s) 179, 388, 574, 1312
BspLI GGNNCC 2 cut(s) 352, 1228
BspMAI CTGCAG 1 cut(s) 1264
BspQI GCTCTTC 1 cut(s) 591
BspT107I GGYRCC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 1019
BsrI ACTGG 1 cut(s) 1113
BssECI CCNNGG 1 cut(s) 772
BssMI GATC 3 cut(s) 169, 535, 1162
BssNI GRCGYC 2 cut(s) 429, 756
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 8 cut(s) 67, 438, 445, 452, 856, 952, 1174, 1241
Bst6I CTCTTC 5 cut(s) 116, 591, 890, 898, 1335
BstACI GRCGYC 2 cut(s) 429, 756
BstC8I GCNNGC 1 cut(s) 668
BstDEI CTNAG 4 cut(s) 166, 396, 582, 1320
BstF5I GGATG 4 cut(s) 121, 274, 613, 1036
BstKTI GATC 3 cut(s) 172, 538, 1165
BstMAI GTCTC 3 cut(s) 405, 1019, 1163
BstMBI GATC 3 cut(s) 169, 535, 1162
BstMWI GCNNNNNNNGC 4 cut(s) 11, 713, 1253, 1262
BstNI CCWGG 1 cut(s) 774
BstNSI RCATGY 2 cut(s) 528, 1205
BstSCI CCNGG 1 cut(s) 772
BstSFI CTRYAG 2 cut(s) 472, 1260
BstV1I GCAGC 5 cut(s) 716, 817, 1243, 1246, 1274
BstX2I RGATCY 1 cut(s) 169
BstXI CCANNNNNNTGG 2 cut(s) 555, 1293
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 3 cut(s) 560, 670, 734
BtsCI GGATG 4 cut(s) 121, 274, 613, 1036
BtsIMutI CAGTG 2 cut(s) 363, 861
Cac8I GCNNGC 1 cut(s) 668
Cfr13I GGNCC 1 cut(s) 558
CseI GACGC 2 cut(s) 418, 745
Csp6I GTAC 3 cut(s) 511, 742, 1153
CspCI CAANNNNNGTGG 2 cut(s) 1008, 1043
CviAII CATG 2 cut(s) 525, 1202
CviQI GTAC 3 cut(s) 511, 742, 1153
DdeI CTNAG 4 cut(s) 166, 396, 582, 1320
DpnI GATC 3 cut(s) 171, 537, 1164
DpnII GATC 3 cut(s) 169, 535, 1162
EaeI YGGCCR 1 cut(s) 732
Eam1104I CTCTTC 5 cut(s) 116, 591, 890, 898, 1335
EarI CTCTTC 5 cut(s) 116, 591, 890, 898, 1335
Eco31I GGTCTC 1 cut(s) 1019
Eco32I GATATC 1 cut(s) 1114
Eco57I CTGAAG 2 cut(s) 342, 387
EcoRII CCWGG 1 cut(s) 772
EcoRV GATATC 1 cut(s) 1114
EcoT22I ATGCAT 1 cut(s) 718
FaeI CATG 2 cut(s) 528, 1205
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FaqI GGGAC 1 cut(s) 635
FatI CATG 2 cut(s) 524, 1201
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 5 cut(s) 705, 831, 1257, 1260, 1263
FokI GGATG 4 cut(s) 128, 281, 620, 1023
Fsp4HI GCNGC 5 cut(s) 705, 831, 1257, 1260, 1263
FspBI CTAG 4 cut(s) 723, 917, 1062, 1166
GluI GCNGC 5 cut(s) 705, 831, 1257, 1260, 1263
HaeIII GGCC 3 cut(s) 560, 670, 734
HgaI GACGC 2 cut(s) 418, 745
Hin1I GRCGYC 2 cut(s) 429, 756
Hin1II CATG 2 cut(s) 528, 1205
HincII GTYRAC 5 cut(s) 283, 441, 448, 466, 852
HindII GTYRAC 5 cut(s) 283, 441, 448, 466, 852
HindIII AAGCTT 2 cut(s) 1245, 1277
HinfI GANTC 4 cut(s) 398, 586, 882, 909
HpaI GTTAAC 4 cut(s) 441, 448, 466, 852
Hpy166II GTNNAC 7 cut(s) 283, 441, 448, 466, 744, 852, 1207
Hpy188I TCNGA 8 cut(s) 169, 397, 434, 497, 790, 864, 898, 914
Hpy188III TCNNGA 5 cut(s) 131, 250, 879, 1117, 1166
Hpy8I GTNNAC 7 cut(s) 283, 441, 448, 466, 744, 852, 1207
HpyAV CCTTC 6 cut(s) 386, 395, 885, 893, 970, 1328
HpyCH4III ACNGT 8 cut(s) 67, 438, 445, 452, 856, 952, 1174, 1241
HpyCH4V TGCA 8 cut(s) 26, 275, 524, 686, 716, 1079, 1214, 1262
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 713, 1253, 1262
HpyF3I CTNAG 4 cut(s) 166, 396, 582, 1320
Hsp92I GRCGYC 2 cut(s) 429, 756
Hsp92II CATG 2 cut(s) 528, 1205
Ksp22I TGATCA 1 cut(s) 535
KspAI GTTAAC 4 cut(s) 441, 448, 466, 852
Kzo9I GATC 3 cut(s) 169, 535, 1162
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 5 cut(s) 716, 817, 1243, 1246, 1274
LweI GCATC 3 cut(s) 262, 568, 1306
MaeI CTAG 4 cut(s) 723, 917, 1062, 1166
MaeIII GTNAC 4 cut(s) 31, 67, 196, 1048
MalI GATC 3 cut(s) 171, 537, 1164
MboI GATC 3 cut(s) 169, 535, 1162
MboII GAAGA 9 cut(s) 133, 335, 608, 656, 877, 880, 885, 1352, 1357
MfeI CAATTG 2 cut(s) 760, 926
MflI RGATCY 1 cut(s) 169
MlsI TGGCCA 1 cut(s) 734
MluNI TGGCCA 1 cut(s) 734
MlyI GAGTC 2 cut(s) 407, 876
MnlI CCTC 7 cut(s) 119, 390, 557, 604, 612, 1081, 1203
Mox20I TGGCCA 1 cut(s) 734
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 2 cut(s) 990, 1361
MscI TGGCCA 1 cut(s) 734
MslI CAYNNNNRTG 1 cut(s) 950
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 1 cut(s) 1265
MspR9I CCNGG 1 cut(s) 774
MunI CAATTG 2 cut(s) 760, 926
Mva1269I GAATGC 2 cut(s) 371, 718
MvaI CCWGG 1 cut(s) 774
MwoI GCNNNNNNNGC 4 cut(s) 11, 713, 1253, 1262
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 169, 535, 1162
NlaIII CATG 2 cut(s) 528, 1205
NlaIV GGNNCC 2 cut(s) 352, 1228
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 2 cut(s) 528, 1205
PciI ACATGT 1 cut(s) 1201
PciSI GCTCTTC 1 cut(s) 591
PctI GAATGC 2 cut(s) 371, 718
PdmI GAANNNNTTC 2 cut(s) 990, 1361
PfeI GAWTC 2 cut(s) 586, 909
PflFI GACNNNGTC 1 cut(s) 1085
PflMI CCANNNNNTGG 1 cut(s) 666
PkrI GCNGC 5 cut(s) 706, 832, 1258, 1261, 1264
PleI GAGTC 2 cut(s) 406, 876
PpsI GAGTC 2 cut(s) 406, 876
PscI ACATGT 1 cut(s) 1201
Psp6I CCWGG 1 cut(s) 772
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 2 cut(s) 352, 1228
PspPI GGNCC 1 cut(s) 558
PstI CTGCAG 1 cut(s) 1264
PsuI RGATCY 1 cut(s) 169
PsyI GACNNNGTC 1 cut(s) 1085
PvuII CAGCTG 1 cut(s) 1265
RsaI GTAC 3 cut(s) 512, 743, 1154
RsaNI GTAC 3 cut(s) 511, 742, 1153
RseI CAYNNNNRTG 1 cut(s) 950
SapI GCTCTTC 1 cut(s) 591
SatI GCNGC 5 cut(s) 705, 831, 1257, 1260, 1263
Sau3AI GATC 3 cut(s) 169, 535, 1162
Sau96I GGNCC 1 cut(s) 558
ScaI AGTACT 1 cut(s) 1154
SchI GAGTC 2 cut(s) 407, 876
ScrFI CCNGG 1 cut(s) 774
SfaNI GCATC 3 cut(s) 262, 568, 1306
SfcI CTRYAG 2 cut(s) 472, 1260
SmiMI CAYNNNNRTG 1 cut(s) 950
SmlI CTYRAG 2 cut(s) 286, 1145
SmoI CTYRAG 2 cut(s) 286, 1145
SpeI ACTAGT 1 cut(s) 916
SsiI CCGC 1 cut(s) 847
SspI AATATT 2 cut(s) 418, 492
SspMI CTAG 4 cut(s) 723, 917, 1062, 1166
StyD4I CCNGG 1 cut(s) 772
TaaI ACNGT 8 cut(s) 67, 438, 445, 452, 856, 952, 1174, 1241
TatI WGTACW 3 cut(s) 510, 741, 1152
TfiI GAWTC 2 cut(s) 586, 909
TscAI CASTG 2 cut(s) 370, 861
TseI GCWGC 5 cut(s) 704, 830, 1256, 1259, 1262
TspDTI ATGAA 5 cut(s) 470, 567, 840, 984, 1025
TspRI CASTG 2 cut(s) 370, 861
Tth111I GACNNNGTC 1 cut(s) 1085
Van91I CCANNNNNTGG 1 cut(s) 666
XapI RAATTY 2 cut(s) 1136, 1360
XbaI TCTAGA 1 cut(s) 1165
XceI RCATGY 2 cut(s) 528, 1205
XmnI GAANNNNTTC 2 cut(s) 990, 1361
XspI CTAG 4 cut(s) 723, 917, 1062, 1166
ZrmI AGTACT 1 cut(s) 1154
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.