Belongs to the short-chain dehydrogenases reductases (SDR) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 735 |
| AciI | CCGC | 1 cut(s) | 98 |
| AclWI | GGATC | 1 cut(s) | 46 |
| AcsI | RAATTY | 2 cut(s) | 543, 826 |
| AcuI | CTGAAG | 3 cut(s) | 360, 443, 459 |
| AfaI | GTAC | 3 cut(s) | 424, 553, 722 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 504, 848 |
| AgsI | TTSAA | 7 cut(s) | 62, 134, 316, 361, 635, 781, 803 |
| AjnI | CCWGG | 1 cut(s) | 751 |
| AjuI | GAANNNNNNNTTGG | 3 cut(s) | 165, 197, 855 |
| AleI | CACNNNNGTG | 1 cut(s) | 98 |
| AluBI | AGCT | 7 cut(s) | 137, 148, 256, 293, 299, 320, 463 |
| AluI | AGCT | 7 cut(s) | 137, 148, 256, 293, 299, 320, 463 |
| AlwI | GGATC | 1 cut(s) | 46 |
| Ama87I | CYCGRG | 1 cut(s) | 166 |
| AoxI | GGCC | 7 cut(s) | 78, 127, 160, 169, 647, 850, 859 |
| ApeKI | GCWGC | 4 cut(s) | 320, 683, 812, 838 |
| ApoI | RAATTY | 2 cut(s) | 543, 826 |
| AspS9I | GGNCC | 2 cut(s) | 169, 851 |
| AsuC2I | CCSGG | 1 cut(s) | 726 |
| AsuHPI | GGTGA | 4 cut(s) | 176, 280, 465, 547 |
| AvaI | CYCGRG | 1 cut(s) | 166 |
| BbvI | GCAGC | 4 cut(s) | 307, 695, 799, 825 |
| BccI | CCATC | 2 cut(s) | 302, 842 |
| BciT130I | CCWGG | 1 cut(s) | 753 |
| BcnI | CCSGG | 1 cut(s) | 726 |
| BfaI | CTAG | 3 cut(s) | 210, 224, 710 |
| BfmI | CTRYAG | 1 cut(s) | 294 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 168, 858 |
| BisI | GCNGC | 4 cut(s) | 321, 684, 813, 839 |
| BlsI | GCNGC | 4 cut(s) | 322, 685, 814, 840 |
| BmcAI | AGTACT | 1 cut(s) | 553 |
| Bme1390I | CCNGG | 2 cut(s) | 726, 753 |
| BmeT110I | CYCGRG | 1 cut(s) | 166 |
| BmgT120I | GGNCC | 2 cut(s) | 169, 851 |
| BmiI | GGNNCC | 1 cut(s) | 853 |
| BmrFI | CCNGG | 2 cut(s) | 726, 753 |
| Bpu10I | CCTNAGC | 1 cut(s) | 569 |
| BpuEI | CTTGAG | 1 cut(s) | 602 |
| BpuMI | CCSGG | 1 cut(s) | 726 |
| BsaJI | CCNNGG | 2 cut(s) | 724, 751 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 504, 848 |
| Bse1I | ACTGG | 2 cut(s) | 91, 790 |
| Bse3DI | GCAATG | 2 cut(s) | 693, 833 |
| BseBI | CCWGG | 1 cut(s) | 753 |
| BseDI | CCNNGG | 2 cut(s) | 724, 751 |
| BseGI | GGATG | 1 cut(s) | 494 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 504, 848 |
| BseMI | GCAATG | 2 cut(s) | 693, 833 |
| BseMII | CTCAG | 2 cut(s) | 257, 562 |
| BseNI | ACTGG | 2 cut(s) | 91, 790 |
| BseXI | GCAGC | 4 cut(s) | 307, 695, 799, 825 |
| Bsh1236I | CGCG | 1 cut(s) | 735 |
| BshFI | GGCC | 7 cut(s) | 80, 129, 162, 171, 649, 852, 861 |
| BsiHKCI | CYCGRG | 1 cut(s) | 166 |
| BsiSI | CCGG | 1 cut(s) | 726 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 504, 848 |
| BsmI | GAATGC | 2 cut(s) | 404, 697 |
| BsnI | GGCC | 7 cut(s) | 80, 129, 162, 171, 649, 852, 861 |
| BsoBI | CYCGRG | 1 cut(s) | 166 |
| Bsp143I | GATC | 1 cut(s) | 38 |
| BspACI | CCGC | 1 cut(s) | 98 |
| BspANI | GGCC | 7 cut(s) | 80, 129, 162, 171, 649, 852, 861 |
| BspCNI | CTCAG | 2 cut(s) | 256, 561 |
| BspFNI | CGCG | 1 cut(s) | 735 |
| BspLI | GGNNCC | 1 cut(s) | 853 |
| BspPI | GGATC | 1 cut(s) | 46 |
| BsrDI | GCAATG | 2 cut(s) | 693, 833 |
| BsrI | ACTGG | 2 cut(s) | 91, 790 |
| BssECI | CCNNGG | 2 cut(s) | 724, 751 |
| BssMI | GATC | 1 cut(s) | 38 |
| Bst2UI | CCWGG | 1 cut(s) | 753 |
| Bst6I | CTCTTC | 2 cut(s) | 116, 570 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 692 |
| BstC8I | GCNNGC | 2 cut(s) | 404, 647 |
| BstDEI | CTNAG | 3 cut(s) | 243, 548, 569 |
| BstF5I | GGATG | 1 cut(s) | 494 |
| BstFNI | CGCG | 1 cut(s) | 735 |
| BstKTI | GATC | 1 cut(s) | 41 |
| BstMBI | GATC | 1 cut(s) | 38 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 168, 206, 326, 335, 692, 858 |
| BstNI | CCWGG | 1 cut(s) | 753 |
| BstSCI | CCNGG | 2 cut(s) | 724, 751 |
| BstSFI | CTRYAG | 1 cut(s) | 294 |
| BstUI | CGCG | 1 cut(s) | 735 |
| BstV1I | GCAGC | 4 cut(s) | 307, 695, 799, 825 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 531 |
| BsuRI | GGCC | 7 cut(s) | 80, 129, 162, 171, 649, 852, 861 |
| BtgZI | GCGATG | 1 cut(s) | 671 |
| BtsCI | GGATG | 1 cut(s) | 494 |
| BtsI | GCAGTG | 1 cut(s) | 93 |
| BtsIMutI | CAGTG | 1 cut(s) | 93 |
| Cac8I | GCNNGC | 2 cut(s) | 404, 647 |
| Cfr13I | GGNCC | 2 cut(s) | 169, 851 |
| CseI | GACGC | 1 cut(s) | 724 |
| Csp6I | GTAC | 3 cut(s) | 423, 552, 721 |
| CviQI | GTAC | 3 cut(s) | 423, 552, 721 |
| DdeI | CTNAG | 3 cut(s) | 243, 548, 569 |
| DpnI | GATC | 1 cut(s) | 40 |
| DpnII | GATC | 1 cut(s) | 38 |
| Eam1104I | CTCTTC | 2 cut(s) | 116, 570 |
| EarI | CTCTTC | 2 cut(s) | 116, 570 |
| Eco147I | AGGCCT | 1 cut(s) | 129 |
| Eco57I | CTGAAG | 3 cut(s) | 360, 443, 459 |
| Eco88I | CYCGRG | 1 cut(s) | 166 |
| EcoRI | GAATTC | 1 cut(s) | 543 |
| EcoRII | CCWGG | 1 cut(s) | 751 |
| EcoT22I | ATGCAT | 1 cut(s) | 697 |
| Fnu4HI | GCNGC | 4 cut(s) | 321, 684, 813, 839 |
| FokI | GGATG | 1 cut(s) | 481 |
| Fsp4HI | GCNGC | 4 cut(s) | 321, 684, 813, 839 |
| FspBI | CTAG | 3 cut(s) | 210, 224, 710 |
| GluI | GCNGC | 4 cut(s) | 321, 684, 813, 839 |
| HaeIII | GGCC | 7 cut(s) | 80, 129, 162, 171, 649, 852, 861 |
| HapII | CCGG | 1 cut(s) | 726 |
| HgaI | GACGC | 1 cut(s) | 724 |
| HincII | GTYRAC | 3 cut(s) | 105, 372, 739 |
| HindII | GTYRAC | 3 cut(s) | 105, 372, 739 |
| HinfI | GANTC | 9 cut(s) | 8, 51, 90, 155, 218, 448, 470, 480, 706 |
| HpaI | GTTAAC | 1 cut(s) | 105 |
| HpaII | CCGG | 1 cut(s) | 726 |
| HphI | GGTGA | 4 cut(s) | 176, 280, 465, 547 |
| Hpy166II | GTNNAC | 5 cut(s) | 105, 271, 372, 629, 739 |
| Hpy188I | TCNGA | 3 cut(s) | 56, 469, 880 |
| Hpy188III | TCNNGA | 1 cut(s) | 823 |
| Hpy8I | GTNNAC | 5 cut(s) | 105, 271, 372, 629, 739 |
| HpyAV | CCTTC | 3 cut(s) | 467, 660, 800 |
| HpyCH4V | TGCA | 6 cut(s) | 26, 110, 323, 406, 695, 815 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 168, 206, 326, 335, 692, 858 |
| HpyF3I | CTNAG | 3 cut(s) | 243, 548, 569 |
| KspAI | GTTAAC | 1 cut(s) | 105 |
| Kzo9I | GATC | 1 cut(s) | 38 |
| LmnI | GCTCC | 1 cut(s) | 290 |
| Lsp1109I | GCAGC | 4 cut(s) | 307, 695, 799, 825 |
| MaeI | CTAG | 3 cut(s) | 210, 224, 710 |
| MaeIII | GTNAC | 1 cut(s) | 31 |
| MalI | GATC | 1 cut(s) | 40 |
| MboI | GATC | 1 cut(s) | 38 |
| MboII | GAAGA | 4 cut(s) | 133, 407, 587, 815 |
| MfeI | CAATTG | 1 cut(s) | 324 |
| MluCI | AATT | 8 cut(s) | 230, 282, 324, 412, 509, 543, 826, 863 |
| MlyI | GAGTC | 1 cut(s) | 164 |
| MnlI | CCTC | 8 cut(s) | 12, 91, 119, 173, 340, 508, 626, 696 |
| Mph1103I | ATGCAT | 1 cut(s) | 697 |
| MseI | TTAA | 4 cut(s) | 68, 104, 389, 486 |
| MslI | CAYNNNNRTG | 1 cut(s) | 98 |
| MspA1I | CMGCKG | 1 cut(s) | 299 |
| MspI | CCGG | 1 cut(s) | 726 |
| MspR9I | CCNGG | 2 cut(s) | 726, 753 |
| MunI | CAATTG | 1 cut(s) | 324 |
| Mva1269I | GAATGC | 2 cut(s) | 404, 697 |
| MvaI | CCWGG | 1 cut(s) | 753 |
| MvnI | CGCG | 1 cut(s) | 735 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 168, 206, 326, 335, 692, 858 |
| NciI | CCSGG | 1 cut(s) | 726 |
| NdeII | GATC | 1 cut(s) | 38 |
| NlaIV | GGNNCC | 1 cut(s) | 853 |
| NmuCI | GTSAC | 1 cut(s) | 31 |
| NsiI | ATGCAT | 1 cut(s) | 697 |
| OliI | CACNNNNGTG | 1 cut(s) | 98 |
| PceI | AGGCCT | 1 cut(s) | 129 |
| PctI | GAATGC | 2 cut(s) | 404, 697 |
| PfeI | GAWTC | 8 cut(s) | 8, 51, 90, 218, 448, 470, 480, 706 |
| PkrI | GCNGC | 4 cut(s) | 322, 685, 814, 840 |
| PleI | GAGTC | 1 cut(s) | 163 |
| PpsI | GAGTC | 1 cut(s) | 163 |
| Psp6I | CCWGG | 1 cut(s) | 751 |
| PspGI | CCWGG | 1 cut(s) | 751 |
| PspN4I | GGNNCC | 1 cut(s) | 853 |
| PspPI | GGNCC | 2 cut(s) | 169, 851 |
| PvuII | CAGCTG | 1 cut(s) | 299 |
| RsaI | GTAC | 3 cut(s) | 424, 553, 722 |
| RsaNI | GTAC | 3 cut(s) | 423, 552, 721 |
| RseI | CAYNNNNRTG | 1 cut(s) | 98 |
| SaqAI | TTAA | 4 cut(s) | 68, 104, 389, 486 |
| SatI | GCNGC | 4 cut(s) | 321, 684, 813, 839 |
| Sau3AI | GATC | 1 cut(s) | 38 |
| Sau96I | GGNCC | 2 cut(s) | 169, 851 |
| ScaI | AGTACT | 1 cut(s) | 553 |
| SchI | GAGTC | 1 cut(s) | 164 |
| ScrFI | CCNGG | 2 cut(s) | 726, 753 |
| SfcI | CTRYAG | 1 cut(s) | 294 |
| SfiI | GGCCNNNNNGGCC | 2 cut(s) | 168, 858 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 98 |
| SmlI | CTYRAG | 1 cut(s) | 617 |
| SmoI | CTYRAG | 1 cut(s) | 617 |
| Sse9I | AATT | 8 cut(s) | 230, 282, 324, 412, 509, 543, 826, 863 |
| SseBI | AGGCCT | 1 cut(s) | 129 |
| SsiI | CCGC | 1 cut(s) | 98 |
| SspMI | CTAG | 3 cut(s) | 210, 224, 710 |
| StuI | AGGCCT | 1 cut(s) | 129 |
| StyD4I | CCNGG | 2 cut(s) | 724, 751 |
| TaqI | TCGA | 2 cut(s) | 83, 258 |
| TaqII | GACCGA | 1 cut(s) | 864 |
| TasI | AATT | 8 cut(s) | 230, 282, 324, 412, 509, 543, 826, 863 |
| TatI | WGTACW | 1 cut(s) | 551 |
| TfiI | GAWTC | 8 cut(s) | 8, 51, 90, 218, 448, 470, 480, 706 |
| Tru1I | TTAA | 4 cut(s) | 68, 104, 389, 486 |
| Tru9I | TTAA | 4 cut(s) | 68, 104, 389, 486 |
| TscAI | CASTG | 1 cut(s) | 100 |
| TseFI | GTSAC | 1 cut(s) | 31 |
| TseI | GCWGC | 4 cut(s) | 320, 683, 812, 838 |
| Tsp45I | GTSAC | 1 cut(s) | 31 |
| TspDTI | ATGAA | 4 cut(s) | 223, 483, 603, 704 |
| TspRI | CASTG | 1 cut(s) | 100 |
| XapI | RAATTY | 2 cut(s) | 543, 826 |
| XspI | CTAG | 3 cut(s) | 210, 224, 710 |
| ZrmI | AGTACT | 1 cut(s) | 553 |
| Zsp2I | ATGCAT | 1 cut(s) | 697 |