Rroxscaffold_4G00301860

Enoyl-(Acyl carrier protein) reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000170
Physical Location & Seq
Reverse (-)
22215569 .. 22221715
6147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301860.1

Sequence Viewer

Length: 1890 bp
ATGGCGGAATCAACTAAGAGGTATGCAGTTGTGACAGGATCAAACAAAGGAATCGGATTCGAAACTGTAAGGCAGTTGGCCTCAAAGGGAATCACAGTGGTGTTAACTGCTAGAGATGAGAAGAGGGGCCTTGAAGCTGTTGAGAAACTGAAAGAATCTGGCCTCTCAGGCCAAGTGAACAATGCAGGAATTGGTGGAGTTATAGTTGATGCTGATGGTTTTAAAGCTGCAATTGAATCTCGTGCTGAAGGAGAACAGATTGATTGGAAAAAGTTGATGACTGAAACTTACGAGTCAACAGAAGAATGCTTGCAAATCAATTATTATGGTGCTAAAAGAACAGCTGAAGCACTAATTCCGCTCCTCCAGCTATCTGATTCACCAAGAATTGTTAATGTTTCATCCTCTATGGGGAAGTTAAAGAACATACCAAGTGATCGAGTGAAAGGAGTTCTTAGTACTGATGTGGAGAACCTAAGCGAAGAGAGTGTAGATGAAGTACTGACAGAGTTTCTAAACGATTTCAAAGAGAATTTACTTGAAAGCAAGGGTTGGCCTTCTATGATGTCAGGCTATACAGTCTCAAAAGCAGAAATGAATGCATATACAAGGATTCTAGCCAAGAAGTACCCCGGTTTTCGCGTCAACTCTGTCTGCCCTGGCTATGTCAAAACAGATATAAACTTCAATACCGGCGTCTTGCCTGTTGAAGAAGGTGCTGCAAGTGTCGTGAATTTAGCATTGCTGCCTAATGATGGCCCCACAGAACATGAAATGTCCTCAATCATCCTCTCTATTCCACTGTCTCATGGAGAAGGAGATGATAGTGGAGGAGACGGTGGAATATGTGACTCGCCATATGTGGTTCCCAAATATGAGGCAGTACATGACTTGTTGTATGCAGTTGTGACAGGATCAAACAAAGGAATCGGATTTGAAACTGTGAGGCAGTTGGCCTCGACTGGAATCACTGTGGTGTTAACTGCTAGAGATGAGAAGAGAGGCCTTGAAGCTGTTGAGAAACTGAAAGAGTCTGGCATCTCGGGCCAAGTAGTTTTTCACCAACTTGATGTGGCTGACCCTGCTAGTATTGATTCTCTAGCACAATTCATCAAAACTCAGTTTGGGAAGCTCGATATTTTGGTGAACAATGCTGGAATTTTTGGAGCTATAGTTGATGTTGATGGTTTTAAAGCTGCAATTGCCTCTGGTGCTGAAGCAGGACAGATTGATTGGGAAAAGTTGTTGACTGAAACTTATGAGTTAACAAAAGAATGCTTGCAAATCAATTATTATGGTGCTAAAAGAACAACTGAAGTCCTGATTCCACTCCTTCAGCTATCTGATTCACCAAGAATCGTTAATGTTTCATCCTCTATGGGGAAATTAGAGAACATACCAAGTGATCGGGTGAAAGGAGTTCTCAGTACTGATGTGGAGAACCTAAGCGAAGAGAGTGTAGATGAAGTATTGACAGAGTTTCTAAACGACCTCAAGGAGAGTTTACTTGAAAGCAAGGGTTGGCCTTCTGCGATGTCAGGCTATATACTCTCAAAAGCAGCAATGAATGCACATACGAGGATTCTAGCCAAGAAGTACCCCAGTTTTCGTGTCAACTGTGTCTGCCCTGGCTATGTCAAAACAGATATAAACTTCAATACTGGTGTCTTGCCTGTTGAAGAAGGTGCTGCAAGTGTCGTGAATTTAGCATTGCTGCCTAATAATGGCTCCACAGGCCAATTCTTTTTTCGGGTCCTCGATGTTGCTGATTTGGCCGGTCAATTTCTGGGTTGCTCTGCTAGTCCAGGGAGGCAGTTGGCAGTGGATGTGTCGCGGGCAGACGACGCCTGTGTTGGGCAGCGGGCGCAATTGGGATTTGGGAGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

629

Amino Acids

67.67

Weight (kDa)

4.89

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 8 - 141 5.9e-11 short chain dehydrogenase
adh_short_C2 PF13561 15 - 149 1.8e-06 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 183 - 239 4.8e-06 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 301 - 464 1.2e-26 short chain dehydrogenase
KR PF08659 302 - 388 1.5e-06 KR domain
adh_short_C2 PF13561 307 - 467 2.1e-17 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 504 - 564 3.9e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01800 AT1G01800 AT2G24190 AT2G24190 AT3G61220 AT3G61220 AT3G61220
fragaria_vesca FvH4_7g13591 FvH4_7g13601 FvH4_7g13602 FvH4_7g13603 FvH4_7g13603 FvH4_7g13603 FvH4_7g13620
malus_domestica MD01G1052500.v1.1 MD01G1052600.v1.1 MD01G1052700.v1.1 MD01G1052800.v1.1 MD01G1053000.v1.1 MD01G1053100.v1.1 MD01G1053200.v1.1 MD01G1053300.v1.1 MD04G1099300.v1.1 MD04G1099400.v1.1 MD04G1099500.v1.1 MD07G1064800.v1.1 MD07G1142200.v1.1 MD07G1142300.v1.1
prunus_persica Prupe.1G171500_v2.0.a1 Prupe.1G171600_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160400_v2.0.a1 Prupe.2G160500_v2.0.a1 Prupe.2G160600_v2.0.a1 Prupe.2G160700_v2.0.a1 Prupe.2G160700_v2.0.a1 Prupe.2G160800_v2.0.a1 Prupe.2G160800_v2.0.a1 Prupe.2G160900_v2.0.a1 Prupe.2G161000_v2.0.a1 Prupe.2G161100_v2.0.a1 Prupe.2G161100_v2.0.a1 Prupe.2G161200_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161400_v2.0.a1 Prupe.2G161500_v2.0.a1 Prupe.2G161600_v2.0.a1 Prupe.2G161600_v2.0.a1 Prupe.2G161800_v2.0.a1 Prupe.2G161900_v2.0.a1 Prupe.2G161900_v2.0.a1 Prupe.2G162200_v2.0.a1 Prupe.2G162300_v2.0.a1 Prupe.2G162300_v2.0.a1 Prupe.4G240700_v2.0.a1 Prupe.4G240800_v2.0.a1
pyrus_communis pycom01g07820 pycom01g07840 pycom01g07850 pycom01g07860 pycom01g07880 pycom01g07900 pycom04g09450 pycom04g09460 pycom07g01290 pycom07g12490 pycom07g12510 pycom07g12570 pycom07g14080
rosa_chinensis RchiOBHm_Chr1g0353391 RchiOBHm_Chr1g0353401 RchiOBHm_Chr1g0353411 RchiOBHm_Chr1g0353421 RchiOBHm_Chr1g0353431 RchiOBHm_Chr1g0353481 RchiOBHm_Chr1g0353511 RchiOBHm_Chr1g0353531 RchiOBHm_Chr1g0353541 RchiOBHm_Chr1g0353571 RchiOBHm_Chr1g0353581 RchiOBHm_Chr1g0353621 RchiOBHm_Chr1g0353661 RchiOBHm_Chr7g0224711 RchiOBHm_Chr7g0224731 RchiOBHm_Chr7g0224751
rosa_laevigata RLG00000001859 RLG00000001863 RLG00000028244 RLG00000028267 RLG00000028269 RLG00000028270 RLG00000028272
rosa_multiflora Rmu_co8260681.1_g000001 Rmu_co8305447.1_g000001 Rmu_co8330951.1_g000001 Rmu_co8341853.1_g000001 Rmu_sc0000144.1_g000019 Rmu_sc0000144.1_g000020 Rmu_sc0000144.1_g000026 Rmu_sc0000687.1_g000012 Rmu_sc0000687.1_g000018 Rmu_sc0000687.1_g000019 Rmu_sc0000687.1_g000020 Rmu_sc0000687.1_g000026 Rmu_sc0001207.1_g000001 Rmu_sc0001207.1_g000012 Rmu_sc0005914.1_g000002 Rmu_sc0005914.1_g000005 Rmu_sc0005914.1_g000007 Rmu_sc0005947.1_g000025 Rmu_sc0007658.1_g000005 Rmu_sc0011028.1_g000012 Rmu_sc0011028.1_g000013 Rmu_sc0026118.1_g000001 Rmu_sc0026975.1_g000001 Rmu_sc0027167.1_g000001 Rmu_sc0042792.1_g000001
rosa_roxburghii Rroxscaffold_3G00234470 Rroxscaffold_4G00301760 Rroxscaffold_4G00301810 Rroxscaffold_4G00301820 Rroxscaffold_4G00301830 Rroxscaffold_4G00301840 Rroxscaffold_4G00301860 Rroxscaffold_4G00301870
rosa_rugosa Rorug01G0235700 Rorug01G0236300 Rorug01G0236600 Rorug01G0236800 Rorug07G0225000.1 Rorug07G0225100
rosa_samantha Rh1AG247900 Rh1AG248000 Rh1AG248400 Rh1AG248500 Rh1AG248600 Rh1AG248800 Rh1AG249000 Rh1AG249100 Rh1BG217700 Rh1BG218000 Rh1BG218400 Rh1BG218600 Rh1BG218800 Rh1BG218900 Rh1BG219200 Rh1DG244000 Rh1DG244300 Rh1DG244800 Rh1DG245000 Rh1DG245200 Rh1DG245300 Rh1DG245600 Rh1DG246200 Rh7BG361100 Rh7CG396500 Rh7CG396700 Rh7DG371200
rosa_wichuraiana Rw1G021600 Rw1G021610 Rw1G021620 Rw1G021640 Rw1G021650 Rw1G021660 Rw1G021680 Rw1G021710 Rw1G021900 Rw7G031330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 361
AccII CGCG 2 cut(s) 642, 1834
AciI CCGC 4 cut(s) 5, 359, 1834, 1861
AclWI GGATC 2 cut(s) 46, 922
AcoI YGGCCR 1 cut(s) 1773
AcsI RAATTY 4 cut(s) 532, 733, 1158, 1702
AcuI CTGAAG 5 cut(s) 267, 366, 1236, 1319, 1335
AcyI GRCGYC 2 cut(s) 696, 1845
AfaI GTAC 6 cut(s) 460, 501, 629, 885, 1429, 1598
AfiI CCNNNNNNNGG 5 cut(s) 411, 755, 1380, 1724, 1854
AjnI CCWGG 3 cut(s) 658, 1627, 1804
AjuI GAANNNNNNNTTGG 4 cut(s) 1041, 1073, 1731, 1763
AleI CACNNNNGTG 2 cut(s) 98, 974
AluBI AGCT 9 cut(s) 137, 227, 344, 370, 1013, 1132, 1169, 1196, 1339
AluI AGCT 9 cut(s) 137, 227, 344, 370, 1013, 1132, 1169, 1196, 1339
Alw26I GTCTC 3 cut(s) 586, 810, 828
AlwI GGATC 2 cut(s) 46, 922
Ama87I CYCGRG 1 cut(s) 1042
ApeKI GCWGC 8 cut(s) 227, 719, 745, 1196, 1559, 1688, 1714, 1858
ApoI RAATTY 4 cut(s) 532, 733, 1158, 1702
AspLEI GCGC 1 cut(s) 1867
AspS9I GGNCC 4 cut(s) 127, 758, 1045, 1753
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 5 cut(s) 372, 1052, 1156, 1341, 1423
AsuII TTCGAA 1 cut(s) 60
AvaI CYCGRG 1 cut(s) 1042
AvaII GGWCC 1 cut(s) 1753
BauI CACGAG 1 cut(s) 240
BbvI GCAGC 8 cut(s) 214, 706, 732, 1183, 1571, 1675, 1701, 1870
BccI CCATC 3 cut(s) 209, 749, 1178
BciT130I CCWGG 3 cut(s) 660, 1629, 1806
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 3 cut(s) 586, 810, 828
BfaI CTAG 7 cut(s) 111, 617, 987, 1086, 1100, 1586, 1800
BfmI CTRYAG 1 cut(s) 1170
BglI GCCNNNNNGGC 1 cut(s) 168
BisI GCNGC 8 cut(s) 228, 720, 746, 1197, 1560, 1689, 1715, 1859
BlsI GCNGC 8 cut(s) 229, 721, 747, 1198, 1561, 1690, 1716, 1860
BmcAI AGTACT 3 cut(s) 460, 501, 1429
Bme1390I CCNGG 4 cut(s) 633, 660, 1629, 1806
Bme18I GGWCC 1 cut(s) 1753
BmeT110I CYCGRG 1 cut(s) 1042
BmgT120I GGNCC 4 cut(s) 127, 758, 1045, 1753
BmiI GGNNCC 5 cut(s) 128, 760, 867, 1729, 1754
BmrFI CCNGG 4 cut(s) 633, 660, 1629, 1806
BmrI ACTGGG 1 cut(s) 1596
BmsI GCATC 2 cut(s) 199, 1047
BmuI ACTGGG 1 cut(s) 1596
BpmI CTGGAG 1 cut(s) 350
Bpu10I CCTNAGC 2 cut(s) 476, 1445
Bpu14I TTCGAA 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 1478
BpuMI CCSGG 1 cut(s) 633
BsaHI GRCGYC 2 cut(s) 696, 1845
BsaJI CCNNGG 4 cut(s) 631, 658, 1627, 1805
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 1158, 1188
Bsc4I CCNNNNNNNGG 5 cut(s) 411, 755, 1380, 1724, 1854
Bse118I RCCGGY 2 cut(s) 692, 1775
Bse1I ACTGG 3 cut(s) 967, 1602, 1666
Bse3DI GCAATG 3 cut(s) 740, 1569, 1709
BseBI CCWGG 3 cut(s) 660, 1629, 1806
BseDI CCNNGG 4 cut(s) 631, 658, 1627, 1805
BseGI GGATG 4 cut(s) 401, 786, 1370, 1831
BseLI CCNNNNNNNGG 5 cut(s) 411, 755, 1380, 1724, 1854
BseMI GCAATG 3 cut(s) 740, 1569, 1709
BseMII CTCAG 3 cut(s) 180, 1133, 1438
BseNI ACTGG 3 cut(s) 967, 1602, 1666
BseRI GAGGAG 2 cut(s) 353, 846
BseXI GCAGC 8 cut(s) 214, 706, 732, 1183, 1571, 1675, 1701, 1870
Bsh1236I CGCG 2 cut(s) 642, 1834
BsiHKCI CYCGRG 1 cut(s) 1042
BsiSI CCGG 3 cut(s) 633, 693, 1776
BslI CCNNNNNNNGG 5 cut(s) 411, 755, 1380, 1724, 1854
BsmAI GTCTC 3 cut(s) 586, 810, 828
BsmBI CGTCTC 1 cut(s) 828
BsmI GAATGC 4 cut(s) 311, 604, 1280, 1573
BsoBI CYCGRG 1 cut(s) 1042
Bsp119I TTCGAA 1 cut(s) 60
Bsp143I GATC 4 cut(s) 38, 436, 914, 1405
BspACI CCGC 4 cut(s) 5, 359, 1834, 1861
BspCNI CTCAG 3 cut(s) 179, 1132, 1437
BspFNI CGCG 2 cut(s) 642, 1834
BspLI GGNNCC 5 cut(s) 128, 760, 867, 1729, 1754
BspPI GGATC 2 cut(s) 46, 922
BspT104I TTCGAA 1 cut(s) 60
BsrBI CCGCTC 1 cut(s) 361
BsrDI GCAATG 3 cut(s) 740, 1569, 1709
BsrFI RCCGGY 2 cut(s) 692, 1775
BsrI ACTGG 3 cut(s) 967, 1602, 1666
BssAI RCCGGY 2 cut(s) 692, 1775
BssECI CCNNGG 4 cut(s) 631, 658, 1627, 1805
BssMI GATC 4 cut(s) 38, 436, 914, 1405
BssNI GRCGYC 2 cut(s) 696, 1845
BssSI CACGAG 1 cut(s) 240
Bst2BI CACGAG 1 cut(s) 240
Bst2UI CCWGG 3 cut(s) 660, 1629, 1806
Bst4CI ACNGT 8 cut(s) 67, 97, 580, 804, 839, 943, 973, 1619
Bst6I CTCTTC 4 cut(s) 116, 477, 992, 1446
BstACI GRCGYC 2 cut(s) 696, 1845
BstAPI GCANNNNNTGC 1 cut(s) 1568
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 4 cut(s) 311, 1280, 1836, 1863
BstDEI CTNAG 7 cut(s) 15, 166, 455, 476, 1119, 1424, 1445
BstF5I GGATG 4 cut(s) 401, 786, 1370, 1831
BstFNI CGCG 2 cut(s) 642, 1834
BstHHI GCGC 1 cut(s) 1867
BstKTI GATC 4 cut(s) 41, 439, 917, 1408
BstMAI GTCTC 3 cut(s) 586, 810, 828
BstMBI GATC 4 cut(s) 38, 436, 914, 1405
BstNI CCWGG 3 cut(s) 660, 1629, 1806
BstSCI CCNGG 4 cut(s) 631, 658, 1627, 1804
BstSFI CTRYAG 1 cut(s) 1170
BstUI CGCG 2 cut(s) 642, 1834
BstV1I GCAGC 8 cut(s) 214, 706, 732, 1183, 1571, 1675, 1701, 1870
BtgZI GCGATG 1 cut(s) 1547
BtsCI GGATG 4 cut(s) 401, 786, 1370, 1831
BtsI GCAGTG 1 cut(s) 1827
BtsIMutI CAGTG 4 cut(s) 102, 800, 969, 1827
Cac8I GCNNGC 4 cut(s) 311, 1280, 1836, 1863
CfoI GCGC 1 cut(s) 1867
Cfr10I RCCGGY 2 cut(s) 692, 1775
Cfr13I GGNCC 4 cut(s) 127, 758, 1045, 1753
CseI GACGC 3 cut(s) 631, 685, 1853
Csp6I GTAC 6 cut(s) 459, 500, 628, 884, 1428, 1597
CviAII CATG 3 cut(s) 770, 809, 887
CviQI GTAC 6 cut(s) 459, 500, 628, 884, 1428, 1597
DdeI CTNAG 7 cut(s) 15, 166, 455, 476, 1119, 1424, 1445
DpnI GATC 4 cut(s) 40, 438, 916, 1407
DpnII GATC 4 cut(s) 38, 436, 914, 1405
DraI TTTAAA 2 cut(s) 223, 1192
EaeI YGGCCR 1 cut(s) 1773
Eam1104I CTCTTC 4 cut(s) 116, 477, 992, 1446
EarI CTCTTC 4 cut(s) 116, 477, 992, 1446
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 1005
Eco47I GGWCC 1 cut(s) 1753
Eco57I CTGAAG 5 cut(s) 267, 366, 1236, 1319, 1335
Eco88I CYCGRG 1 cut(s) 1042
EcoO109I RGGNCCY 2 cut(s) 127, 1753
EcoRII CCWGG 3 cut(s) 658, 1627, 1804
EcoT22I ATGCAT 1 cut(s) 604
Esp3I CGTCTC 1 cut(s) 828
FaeI CATG 3 cut(s) 773, 812, 890
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FatI CATG 3 cut(s) 769, 808, 886
FauI CCCGC 2 cut(s) 1827, 1854
FauNDI CATATG 1 cut(s) 859
Fnu4HI GCNGC 8 cut(s) 228, 720, 746, 1197, 1560, 1689, 1715, 1859
FokI GGATG 4 cut(s) 388, 773, 1357, 1838
Fsp4HI GCNGC 8 cut(s) 228, 720, 746, 1197, 1560, 1689, 1715, 1859
FspBI CTAG 7 cut(s) 111, 617, 987, 1086, 1100, 1586, 1800
GlaI GCGC 1 cut(s) 1866
GluI GCNGC 8 cut(s) 228, 720, 746, 1197, 1560, 1689, 1715, 1859
GsuI CTGGAG 1 cut(s) 350
HapII CCGG 3 cut(s) 633, 693, 1776
HgaI GACGC 3 cut(s) 631, 685, 1853
HhaI GCGC 1 cut(s) 1867
Hin1I GRCGYC 2 cut(s) 696, 1845
Hin1II CATG 3 cut(s) 773, 812, 890
Hin6I GCGC 1 cut(s) 1865
HinP1I GCGC 1 cut(s) 1865
HincII GTYRAC 7 cut(s) 105, 297, 646, 981, 1248, 1266, 1615
HindII GTYRAC 7 cut(s) 105, 297, 646, 981, 1248, 1266, 1615
HpaI GTTAAC 3 cut(s) 105, 981, 1266
HpaII CCGG 3 cut(s) 633, 693, 1776
HphI GGTGA 5 cut(s) 372, 1052, 1156, 1341, 1423
Hpy188I TCNGA 4 cut(s) 56, 376, 932, 1345
Hpy188III TCNNGA 3 cut(s) 730, 1321, 1699
Hpy99I CGWCG 1 cut(s) 1847
HpyAV CCTTC 7 cut(s) 242, 567, 707, 809, 1343, 1536, 1676
HpyCH4III ACNGT 8 cut(s) 67, 97, 580, 804, 839, 943, 973, 1619
HpyF3I CTNAG 7 cut(s) 15, 166, 455, 476, 1119, 1424, 1445
Hsp92I GRCGYC 2 cut(s) 696, 1845
Hsp92II CATG 3 cut(s) 773, 812, 890
HspAI GCGC 1 cut(s) 1865
KspAI GTTAAC 3 cut(s) 105, 981, 1266
Kzo9I GATC 4 cut(s) 38, 436, 914, 1405
LmnI GCTCC 3 cut(s) 366, 1166, 1733
Lsp1109I GCAGC 8 cut(s) 214, 706, 732, 1183, 1571, 1675, 1701, 1870
LweI GCATC 2 cut(s) 199, 1047
MaeI CTAG 7 cut(s) 111, 617, 987, 1086, 1100, 1586, 1800
MaeIII GTNAC 3 cut(s) 31, 848, 907
MalI GATC 4 cut(s) 40, 438, 916, 1407
MbiI CCGCTC 1 cut(s) 361
MboI GATC 4 cut(s) 38, 436, 914, 1405
MboII GAAGA 7 cut(s) 133, 314, 494, 722, 1009, 1463, 1691
MfeI CAATTG 3 cut(s) 231, 1200, 1868
MlyI GAGTC 3 cut(s) 302, 845, 1040
Mph1103I ATGCAT 1 cut(s) 604
MseI TTAA 8 cut(s) 104, 222, 393, 419, 980, 1191, 1265, 1362
MslI CAYNNNNRTG 2 cut(s) 98, 974
MspA1I CMGCKG 2 cut(s) 344, 1861
MspI CCGG 3 cut(s) 633, 693, 1776
MspR9I CCNGG 4 cut(s) 633, 660, 1629, 1806
MunI CAATTG 3 cut(s) 231, 1200, 1868
Mva1269I GAATGC 4 cut(s) 311, 604, 1280, 1573
MvaI CCWGG 3 cut(s) 660, 1629, 1806
MvnI CGCG 2 cut(s) 642, 1834
NciI CCSGG 1 cut(s) 633
NdeI CATATG 1 cut(s) 859
NdeII GATC 4 cut(s) 38, 436, 914, 1405
NlaIII CATG 3 cut(s) 773, 812, 890
NlaIV GGNNCC 5 cut(s) 128, 760, 867, 1729, 1754
NmuCI GTSAC 3 cut(s) 31, 848, 907
NsiI ATGCAT 1 cut(s) 604
NspV TTCGAA 1 cut(s) 60
OliI CACNNNNGTG 2 cut(s) 98, 974
PceI AGGCCT 1 cut(s) 1005
PctI GAATGC 4 cut(s) 311, 604, 1280, 1573
PkrI GCNGC 8 cut(s) 229, 721, 747, 1198, 1561, 1690, 1716, 1860
PleI GAGTC 3 cut(s) 301, 845, 1039
PpsI GAGTC 3 cut(s) 301, 845, 1039
PpuMI RGGWCCY 1 cut(s) 1753
Psp5II RGGWCCY 1 cut(s) 1753
Psp6I CCWGG 3 cut(s) 658, 1627, 1804
PspGI CCWGG 3 cut(s) 658, 1627, 1804
PspN4I GGNNCC 5 cut(s) 128, 760, 867, 1729, 1754
PspPI GGNCC 4 cut(s) 127, 758, 1045, 1753
PspPPI RGGWCCY 1 cut(s) 1753
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 6 cut(s) 460, 501, 629, 885, 1429, 1598
RsaNI GTAC 6 cut(s) 459, 500, 628, 884, 1428, 1597
RseI CAYNNNNRTG 2 cut(s) 98, 974
SaqAI TTAA 8 cut(s) 104, 222, 393, 419, 980, 1191, 1265, 1362
SatI GCNGC 8 cut(s) 228, 720, 746, 1197, 1560, 1689, 1715, 1859
Sau3AI GATC 4 cut(s) 38, 436, 914, 1405
Sau96I GGNCC 4 cut(s) 127, 758, 1045, 1753
ScaI AGTACT 3 cut(s) 460, 501, 1429
SchI GAGTC 3 cut(s) 302, 845, 1040
ScrFI CCNGG 4 cut(s) 633, 660, 1629, 1806
SfaNI GCATC 2 cut(s) 199, 1047
SfcI CTRYAG 1 cut(s) 1170
SfiI GGCCNNNNNGGCC 1 cut(s) 168
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 1 cut(s) 1753
SmiMI CAYNNNNRTG 2 cut(s) 98, 974
SmlI CTYRAG 1 cut(s) 1493
SmoI CTYRAG 1 cut(s) 1493
SseBI AGGCCT 1 cut(s) 1005
SsiI CCGC 4 cut(s) 5, 359, 1834, 1861
SspMI CTAG 7 cut(s) 111, 617, 987, 1086, 1100, 1586, 1800
StuI AGGCCT 1 cut(s) 1005
StyD4I CCNGG 4 cut(s) 631, 658, 1627, 1804
TaaI ACNGT 8 cut(s) 67, 97, 580, 804, 839, 943, 973, 1619
TaqI TCGA 5 cut(s) 60, 439, 959, 1134, 1758
TatI WGTACW 4 cut(s) 458, 499, 883, 1427
Tru1I TTAA 8 cut(s) 104, 222, 393, 419, 980, 1191, 1265, 1362
Tru9I TTAA 8 cut(s) 104, 222, 393, 419, 980, 1191, 1265, 1362
TscAI CASTG 4 cut(s) 102, 807, 976, 1827
TseFI GTSAC 3 cut(s) 31, 848, 907
TseI GCWGC 8 cut(s) 227, 719, 745, 1196, 1559, 1688, 1714, 1858
Tsp45I GTSAC 3 cut(s) 31, 848, 907
TspDTI ATGAA 8 cut(s) 390, 510, 611, 786, 1099, 1359, 1479, 1580
TspRI CASTG 4 cut(s) 102, 807, 976, 1827
VpaK11BI GGWCC 1 cut(s) 1753
XapI RAATTY 4 cut(s) 532, 733, 1158, 1702
XspI CTAG 7 cut(s) 111, 617, 987, 1086, 1100, 1586, 1800
ZrmI AGTACT 3 cut(s) 460, 501, 1429
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.