Belongs to the short-chain dehydrogenases reductases (SDR) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 3 cut(s) | 46, 285, 298 |
| AcsI | RAATTY | 3 cut(s) | 534, 805, 851 |
| AfaI | GTAC | 2 cut(s) | 509, 710 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 249, 836 |
| AflII | CTTAAG | 1 cut(s) | 596 |
| AgsI | TTSAA | 5 cut(s) | 134, 623, 763, 769, 791 |
| AjnI | CCWGG | 1 cut(s) | 712 |
| AluBI | AGCT | 5 cut(s) | 137, 256, 320, 416, 454 |
| AluI | AGCT | 5 cut(s) | 137, 256, 320, 416, 454 |
| AlwI | GGATC | 3 cut(s) | 46, 285, 298 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 329 |
| AoxI | GGCC | 3 cut(s) | 160, 635, 838 |
| ApeKI | GCWGC | 4 cut(s) | 320, 329, 800, 826 |
| ApoI | RAATTY | 3 cut(s) | 534, 805, 851 |
| AspS9I | GGNCC | 1 cut(s) | 839 |
| AsuC2I | CCSGG | 1 cut(s) | 741 |
| AsuHPI | GGTGA | 2 cut(s) | 280, 456 |
| AsuII | TTCGAA | 1 cut(s) | 60 |
| AxyI | CCTNAGG | 1 cut(s) | 557 |
| BamHI | GGATCC | 1 cut(s) | 290 |
| BbvI | GCAGC | 4 cut(s) | 307, 316, 787, 813 |
| BccI | CCATC | 2 cut(s) | 302, 830 |
| BciT130I | CCWGG | 1 cut(s) | 714 |
| BcnI | CCSGG | 1 cut(s) | 741 |
| BfaI | CTAG | 3 cut(s) | 111, 210, 698 |
| BfmI | CTRYAG | 1 cut(s) | 321 |
| BfrI | CTTAAG | 1 cut(s) | 596 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 846 |
| BisI | GCNGC | 4 cut(s) | 321, 330, 801, 827 |
| BlsI | GCNGC | 4 cut(s) | 322, 331, 802, 828 |
| Bme1390I | CCNGG | 2 cut(s) | 714, 741 |
| BmgT120I | GGNCC | 1 cut(s) | 839 |
| BmiI | GGNNCC | 1 cut(s) | 292 |
| BmrFI | CCNGG | 2 cut(s) | 714, 741 |
| BmrI | ACTGGG | 2 cut(s) | 238, 825 |
| BmsI | GCATC | 1 cut(s) | 292 |
| BmuI | ACTGGG | 2 cut(s) | 238, 825 |
| BpmI | CTGGAG | 1 cut(s) | 354 |
| Bpu14I | TTCGAA | 1 cut(s) | 60 |
| BpuEI | CTTGAG | 1 cut(s) | 470 |
| BpuMI | CCSGG | 1 cut(s) | 741 |
| BsaJI | CCNNGG | 2 cut(s) | 712, 739 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 249, 836 |
| Bse118I | RCCGGY | 1 cut(s) | 773 |
| Bse1I | ACTGG | 3 cut(s) | 244, 363, 831 |
| Bse21I | CCTNAGG | 1 cut(s) | 557 |
| BseBI | CCWGG | 1 cut(s) | 714 |
| BseDI | CCNNGG | 2 cut(s) | 712, 739 |
| BseGI | GGATG | 2 cut(s) | 425, 485 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 249, 836 |
| BseMII | CTCAG | 2 cut(s) | 180, 548 |
| BseNI | ACTGG | 3 cut(s) | 244, 363, 831 |
| BseXI | GCAGC | 4 cut(s) | 307, 316, 787, 813 |
| BshFI | GGCC | 3 cut(s) | 162, 637, 840 |
| BsiSI | CCGG | 2 cut(s) | 741, 774 |
| BslFI | GGGAC | 1 cut(s) | 141 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 249, 836 |
| BsmFI | GGGAC | 1 cut(s) | 141 |
| BsmI | GAATGC | 1 cut(s) | 395 |
| BsnI | GGCC | 3 cut(s) | 162, 637, 840 |
| Bsp119I | TTCGAA | 1 cut(s) | 60 |
| Bsp143I | GATC | 2 cut(s) | 38, 290 |
| BspANI | GGCC | 3 cut(s) | 162, 637, 840 |
| BspCNI | CTCAG | 2 cut(s) | 179, 549 |
| BspLI | GGNNCC | 1 cut(s) | 292 |
| BspMAI | CTGCAG | 1 cut(s) | 325 |
| BspPI | GGATC | 3 cut(s) | 46, 285, 298 |
| BspT104I | TTCGAA | 1 cut(s) | 60 |
| BspTI | CTTAAG | 1 cut(s) | 596 |
| BsrFI | RCCGGY | 1 cut(s) | 773 |
| BsrI | ACTGG | 3 cut(s) | 244, 363, 831 |
| BssAI | RCCGGY | 1 cut(s) | 773 |
| BssECI | CCNNGG | 2 cut(s) | 712, 739 |
| BssMI | GATC | 2 cut(s) | 38, 290 |
| Bst2UI | CCWGG | 1 cut(s) | 714 |
| Bst4CI | ACNGT | 4 cut(s) | 67, 97, 661, 731 |
| Bst6I | CTCTTC | 2 cut(s) | 116, 558 |
| BstAFI | CTTAAG | 1 cut(s) | 596 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 329, 680 |
| BstBI | TTCGAA | 1 cut(s) | 60 |
| BstC8I | GCNNGC | 2 cut(s) | 395, 635 |
| BstDEI | CTNAG | 2 cut(s) | 166, 557 |
| BstF5I | GGATG | 2 cut(s) | 425, 485 |
| BstKTI | GATC | 2 cut(s) | 41, 293 |
| BstMBI | GATC | 2 cut(s) | 38, 290 |
| BstNI | CCWGG | 1 cut(s) | 714 |
| BstNSI | RCATGY | 1 cut(s) | 514 |
| BstSCI | CCNGG | 2 cut(s) | 712, 739 |
| BstSFI | CTRYAG | 1 cut(s) | 321 |
| BstV1I | GCAGC | 4 cut(s) | 307, 316, 787, 813 |
| BstX2I | RGATCY | 1 cut(s) | 290 |
| BstYI | RGATCY | 1 cut(s) | 290 |
| Bsu36I | CCTNAGG | 1 cut(s) | 557 |
| BsuRI | GGCC | 3 cut(s) | 162, 637, 840 |
| BtsCI | GGATG | 2 cut(s) | 425, 485 |
| BtsI | GCAGTG | 1 cut(s) | 821 |
| BtsIMutI | CAGTG | 3 cut(s) | 93, 821, 838 |
| Cac8I | GCNNGC | 2 cut(s) | 395, 635 |
| CaiI | CAGNNNCTG | 1 cut(s) | 329 |
| Cfr10I | RCCGGY | 1 cut(s) | 773 |
| Cfr13I | GGNCC | 1 cut(s) | 839 |
| Csp6I | GTAC | 2 cut(s) | 508, 709 |
| CviAII | CATG | 1 cut(s) | 511 |
| CviQI | GTAC | 2 cut(s) | 508, 709 |
| DdeI | CTNAG | 2 cut(s) | 166, 557 |
| DpnI | GATC | 2 cut(s) | 40, 292 |
| DpnII | GATC | 2 cut(s) | 38, 290 |
| Eam1104I | CTCTTC | 2 cut(s) | 116, 558 |
| EarI | CTCTTC | 2 cut(s) | 116, 558 |
| Eco32I | GATATC | 1 cut(s) | 262 |
| Eco81I | CCTNAGG | 1 cut(s) | 557 |
| EcoRII | CCWGG | 1 cut(s) | 712 |
| EcoRV | GATATC | 1 cut(s) | 262 |
| EcoT22I | ATGCAT | 1 cut(s) | 685 |
| FaeI | CATG | 1 cut(s) | 514 |
| FaqI | GGGAC | 1 cut(s) | 141 |
| FatI | CATG | 1 cut(s) | 510 |
| Fnu4HI | GCNGC | 4 cut(s) | 321, 330, 801, 827 |
| FokI | GGATG | 2 cut(s) | 412, 472 |
| Fsp4HI | GCNGC | 4 cut(s) | 321, 330, 801, 827 |
| FspBI | CTAG | 3 cut(s) | 111, 210, 698 |
| GluI | GCNGC | 4 cut(s) | 321, 330, 801, 827 |
| GsuI | CTGGAG | 1 cut(s) | 354 |
| HaeIII | GGCC | 3 cut(s) | 162, 637, 840 |
| HapII | CCGG | 2 cut(s) | 741, 774 |
| Hin1II | CATG | 1 cut(s) | 514 |
| HincII | GTYRAC | 1 cut(s) | 727 |
| HindII | GTYRAC | 1 cut(s) | 727 |
| HinfI | GANTC | 4 cut(s) | 57, 90, 461, 694 |
| HpaI | GTTAAC | 1 cut(s) | 727 |
| HpaII | CCGG | 2 cut(s) | 741, 774 |
| HphI | GGTGA | 2 cut(s) | 280, 456 |
| Hpy166II | GTNNAC | 3 cut(s) | 271, 617, 727 |
| Hpy188I | TCNGA | 3 cut(s) | 430, 460, 868 |
| Hpy188III | TCNNGA | 1 cut(s) | 250 |
| Hpy8I | GTNNAC | 3 cut(s) | 271, 617, 727 |
| HpyAV | CCTTC | 3 cut(s) | 648, 788, 852 |
| HpyCH4III | ACNGT | 4 cut(s) | 67, 97, 661, 731 |
| HpyCH4V | TGCA | 6 cut(s) | 26, 278, 323, 397, 683, 803 |
| HpyF3I | CTNAG | 2 cut(s) | 166, 557 |
| Hsp92II | CATG | 1 cut(s) | 514 |
| KspAI | GTTAAC | 1 cut(s) | 727 |
| Kzo9I | GATC | 2 cut(s) | 38, 290 |
| LmnI | GCTCC | 1 cut(s) | 413 |
| Lsp1109I | GCAGC | 4 cut(s) | 307, 316, 787, 813 |
| LweI | GCATC | 1 cut(s) | 292 |
| MaeI | CTAG | 3 cut(s) | 111, 210, 698 |
| MaeIII | GTNAC | 2 cut(s) | 31, 361 |
| MalI | GATC | 2 cut(s) | 40, 292 |
| MboI | GATC | 2 cut(s) | 38, 290 |
| MboII | GAAGA | 5 cut(s) | 133, 398, 575, 633, 803 |
| MflI | RGATCY | 1 cut(s) | 290 |
| MnlI | CCTC | 8 cut(s) | 12, 117, 173, 329, 499, 552, 604, 807 |
| Mph1103I | ATGCAT | 1 cut(s) | 685 |
| MseI | TTAA | 7 cut(s) | 83, 104, 380, 503, 528, 597, 726 |
| MspCI | CTTAAG | 1 cut(s) | 596 |
| MspI | CCGG | 2 cut(s) | 741, 774 |
| MspR9I | CCNGG | 2 cut(s) | 714, 741 |
| Mva1269I | GAATGC | 1 cut(s) | 395 |
| MvaI | CCWGG | 1 cut(s) | 714 |
| NciI | CCSGG | 1 cut(s) | 741 |
| NdeII | GATC | 2 cut(s) | 38, 290 |
| NlaIII | CATG | 1 cut(s) | 514 |
| NlaIV | GGNNCC | 1 cut(s) | 292 |
| NsiI | ATGCAT | 1 cut(s) | 685 |
| NspI | RCATGY | 1 cut(s) | 514 |
| NspV | TTCGAA | 1 cut(s) | 60 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 255 |
| PctI | GAATGC | 1 cut(s) | 395 |
| PfeI | GAWTC | 4 cut(s) | 57, 90, 461, 694 |
| PkrI | GCNGC | 4 cut(s) | 322, 331, 802, 828 |
| Psp6I | CCWGG | 1 cut(s) | 712 |
| PspGI | CCWGG | 1 cut(s) | 712 |
| PspN4I | GGNNCC | 1 cut(s) | 292 |
| PspPI | GGNCC | 1 cut(s) | 839 |
| PstI | CTGCAG | 1 cut(s) | 325 |
| PstNI | CAGNNNCTG | 1 cut(s) | 329 |
| PsuI | RGATCY | 1 cut(s) | 290 |
| RsaI | GTAC | 2 cut(s) | 509, 710 |
| RsaNI | GTAC | 2 cut(s) | 508, 709 |
| SaqAI | TTAA | 7 cut(s) | 83, 104, 380, 503, 528, 597, 726 |
| SatI | GCNGC | 4 cut(s) | 321, 330, 801, 827 |
| Sau3AI | GATC | 2 cut(s) | 38, 290 |
| Sau96I | GGNCC | 1 cut(s) | 839 |
| ScrFI | CCNGG | 2 cut(s) | 714, 741 |
| SfaNI | GCATC | 1 cut(s) | 292 |
| SfcI | CTRYAG | 1 cut(s) | 321 |
| SfuI | TTCGAA | 1 cut(s) | 60 |
| SmlI | CTYRAG | 2 cut(s) | 449, 596 |
| SmoI | CTYRAG | 2 cut(s) | 449, 596 |
| SspMI | CTAG | 3 cut(s) | 111, 210, 698 |
| StyD4I | CCNGG | 2 cut(s) | 712, 739 |
| TaaI | ACNGT | 4 cut(s) | 67, 97, 661, 731 |
| TaqI | TCGA | 2 cut(s) | 60, 258 |
| TaqII | GACCGA | 1 cut(s) | 852 |
| TatI | WGTACW | 1 cut(s) | 507 |
| TfiI | GAWTC | 4 cut(s) | 57, 90, 461, 694 |
| Tru1I | TTAA | 7 cut(s) | 83, 104, 380, 503, 528, 597, 726 |
| Tru9I | TTAA | 7 cut(s) | 83, 104, 380, 503, 528, 597, 726 |
| TscAI | CASTG | 3 cut(s) | 100, 828, 838 |
| TseI | GCWGC | 4 cut(s) | 320, 329, 800, 826 |
| TspDTI | ATGAA | 4 cut(s) | 223, 390, 474, 591 |
| TspRI | CASTG | 3 cut(s) | 100, 828, 838 |
| Vha464I | CTTAAG | 1 cut(s) | 596 |
| XapI | RAATTY | 3 cut(s) | 534, 805, 851 |
| XceI | RCATGY | 1 cut(s) | 514 |
| XspI | CTAG | 3 cut(s) | 111, 210, 698 |
| Zsp2I | ATGCAT | 1 cut(s) | 685 |