Rroxscaffold_4G00301870

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000170
Physical Location & Seq
Reverse (-)
22223521 .. 22225004
1484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301870.1

Sequence Viewer

Length: 774 bp
ATGGCAAAATCAACCAAGTGGTATGCAGTTGTGACAGGATCGAACAAAGGAATCGGATTCGAAACTGTAAGGCAGTTGGCCCCAAAGGGAATCACAGTGGTGTTAACTGCTAGAGATGAGAAGAGGGGCCTTGAAGCTGTTGAGAAACTGAAAGAGTCTGGCCTCTCAGGCCAAGTAGTTTTTCACCAACTTGATGTGGCTGACCCTGCTAGTATTGCTCCTCTGGCACAATTCATCGAGACCCAGTTCGGGAAACTAGATATTTTGGTGAACAATGCAGGGATTGCTGGGGTTATAGTTGATGCTGATCGTTTGAAAGCTGCATCTGAATATGGTGCTCTATCTGATTCACCAAGAATTGTTAATGTTTCATCATCTTTGGGGAAGTTAAAGAACATACCAAGTGATCGAGTGAAAGGAGTTCTTAGTACGGATGTGGAGAACCTAAGTGAAGAGAGTGTAGACGAAGTACTGACAGAGTTTCTAAACGACCTCAAGGAGAATTTACTTGAGAGCAAGGGTTGGCCTTCAACTAAGTCATGGTATACAGTCTCAAAAGCAGCAATGAATGCATATACAAGGATTCTAGCCAAGAAGTACCACGGTTTTCGCGTCAACTCTGTCTGCCCTGGCTATGTCAAAACAGATATAACCTTCAATACCGGCATCCTGCCTGTTGAAGAAGGTGCTGCAAGTGTCGTGAATTTAGCATTACTTCCTAATGATGGCGCCACCGGCCAATTCTTTCTTCGGTCTGAAGTACAAAGACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

257

Amino Acids

27.76

Weight (kDa)

6.2

Isoelectric Point (pI)

26.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 9 - 98 5.6e-22 short chain dehydrogenase
KR PF08659 10 - 96 1.5e-06 KR domain
adh_short_C2 PF13561 15 - 100 2.2e-16 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 172 - 222 4.7e-09 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 179 - 218 1.2e-06 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01800 AT1G01800 AT2G24190 AT2G24190 AT3G61220 AT3G61220 AT3G61220
fragaria_vesca FvH4_7g13591 FvH4_7g13601 FvH4_7g13602 FvH4_7g13603 FvH4_7g13603 FvH4_7g13603 FvH4_7g13620
malus_domestica MD01G1052500.v1.1 MD01G1052600.v1.1 MD01G1052700.v1.1 MD01G1052800.v1.1 MD01G1053000.v1.1 MD01G1053100.v1.1 MD01G1053200.v1.1 MD01G1053300.v1.1 MD04G1099300.v1.1 MD04G1099400.v1.1 MD04G1099500.v1.1 MD07G1064800.v1.1 MD07G1142200.v1.1 MD07G1142300.v1.1
prunus_persica Prupe.1G171500_v2.0.a1 Prupe.1G171600_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160100_v2.0.a1 Prupe.2G160400_v2.0.a1 Prupe.2G160500_v2.0.a1 Prupe.2G160600_v2.0.a1 Prupe.2G160700_v2.0.a1 Prupe.2G160700_v2.0.a1 Prupe.2G160800_v2.0.a1 Prupe.2G160800_v2.0.a1 Prupe.2G160900_v2.0.a1 Prupe.2G161000_v2.0.a1 Prupe.2G161100_v2.0.a1 Prupe.2G161100_v2.0.a1 Prupe.2G161200_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161300_v2.0.a1 Prupe.2G161400_v2.0.a1 Prupe.2G161500_v2.0.a1 Prupe.2G161600_v2.0.a1 Prupe.2G161600_v2.0.a1 Prupe.2G161800_v2.0.a1 Prupe.2G161900_v2.0.a1 Prupe.2G161900_v2.0.a1 Prupe.2G162200_v2.0.a1 Prupe.2G162300_v2.0.a1 Prupe.2G162300_v2.0.a1 Prupe.4G240700_v2.0.a1 Prupe.4G240800_v2.0.a1
pyrus_communis pycom01g07820 pycom01g07840 pycom01g07850 pycom01g07860 pycom01g07880 pycom01g07900 pycom04g09450 pycom04g09460 pycom07g01290 pycom07g12490 pycom07g12510 pycom07g12570 pycom07g14080
rosa_chinensis RchiOBHm_Chr1g0353391 RchiOBHm_Chr1g0353401 RchiOBHm_Chr1g0353411 RchiOBHm_Chr1g0353421 RchiOBHm_Chr1g0353431 RchiOBHm_Chr1g0353481 RchiOBHm_Chr1g0353511 RchiOBHm_Chr1g0353531 RchiOBHm_Chr1g0353541 RchiOBHm_Chr1g0353571 RchiOBHm_Chr1g0353581 RchiOBHm_Chr1g0353621 RchiOBHm_Chr1g0353661 RchiOBHm_Chr7g0224711 RchiOBHm_Chr7g0224731 RchiOBHm_Chr7g0224751
rosa_laevigata RLG00000001859 RLG00000001863 RLG00000028244 RLG00000028267 RLG00000028269 RLG00000028270 RLG00000028272
rosa_multiflora Rmu_co8260681.1_g000001 Rmu_co8305447.1_g000001 Rmu_co8330951.1_g000001 Rmu_co8341853.1_g000001 Rmu_sc0000144.1_g000019 Rmu_sc0000144.1_g000020 Rmu_sc0000144.1_g000026 Rmu_sc0000687.1_g000012 Rmu_sc0000687.1_g000018 Rmu_sc0000687.1_g000019 Rmu_sc0000687.1_g000020 Rmu_sc0000687.1_g000026 Rmu_sc0001207.1_g000001 Rmu_sc0001207.1_g000012 Rmu_sc0005914.1_g000002 Rmu_sc0005914.1_g000005 Rmu_sc0005914.1_g000007 Rmu_sc0005947.1_g000025 Rmu_sc0007658.1_g000005 Rmu_sc0011028.1_g000012 Rmu_sc0011028.1_g000013 Rmu_sc0026118.1_g000001 Rmu_sc0026975.1_g000001 Rmu_sc0027167.1_g000001 Rmu_sc0042792.1_g000001
rosa_roxburghii Rroxscaffold_3G00234470 Rroxscaffold_4G00301760 Rroxscaffold_4G00301810 Rroxscaffold_4G00301820 Rroxscaffold_4G00301830 Rroxscaffold_4G00301840 Rroxscaffold_4G00301860 Rroxscaffold_4G00301870
rosa_rugosa Rorug01G0235700 Rorug01G0236300 Rorug01G0236600 Rorug01G0236800 Rorug07G0225000.1 Rorug07G0225100
rosa_samantha Rh1AG247900 Rh1AG248000 Rh1AG248400 Rh1AG248500 Rh1AG248600 Rh1AG248800 Rh1AG249000 Rh1AG249100 Rh1BG217700 Rh1BG218000 Rh1BG218400 Rh1BG218600 Rh1BG218800 Rh1BG218900 Rh1BG219200 Rh1DG244000 Rh1DG244300 Rh1DG244800 Rh1DG245000 Rh1DG245200 Rh1DG245300 Rh1DG245600 Rh1DG246200 Rh7BG361100 Rh7CG396500 Rh7CG396700 Rh7DG371200
rosa_wichuraiana Rw1G021600 Rw1G021610 Rw1G021620 Rw1G021640 Rw1G021650 Rw1G021660 Rw1G021680 Rw1G021710 Rw1G021900 Rw7G031330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 728
AccI GTMKAC 2 cut(s) 462, 545
AccII CGCG 1 cut(s) 612
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 1 cut(s) 736
AcsI RAATTY 2 cut(s) 502, 703
AcyI GRCGYC 1 cut(s) 729
AfaI GTAC 4 cut(s) 430, 471, 599, 762
AfiI CCNNNNNNNGG 2 cut(s) 249, 725
AgsI TTSAA 5 cut(s) 134, 316, 531, 658, 680
AjnI CCWGG 1 cut(s) 628
AjuI GAANNNNNNNTTGG 4 cut(s) 165, 197, 732, 764
AleI CACNNNNGTG 1 cut(s) 98
AluBI AGCT 2 cut(s) 137, 320
AluI AGCT 2 cut(s) 137, 320
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 2 cut(s) 233, 556
AlwI GGATC 1 cut(s) 46
AoxI GGCC 6 cut(s) 78, 127, 160, 169, 524, 736
ApeKI GCWGC 3 cut(s) 320, 560, 689
ApoI RAATTY 2 cut(s) 502, 703
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 2 cut(s) 79, 127
AsuHPI GGTGA 3 cut(s) 176, 280, 342
AsuII TTCGAA 1 cut(s) 60
BanI GGYRCC 1 cut(s) 728
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 3 cut(s) 307, 572, 676
BccI CCATC 1 cut(s) 719
BciT130I CCWGG 1 cut(s) 630
BcoDI GTCTC 2 cut(s) 233, 556
BfaI CTAG 4 cut(s) 111, 210, 257, 587
BfoI RGCGCY 1 cut(s) 732
BglI GCCNNNNNGGC 1 cut(s) 168
BisI GCNGC 3 cut(s) 321, 561, 690
BlsI GCNGC 3 cut(s) 322, 562, 691
BmcAI AGTACT 1 cut(s) 471
Bme1390I CCNGG 1 cut(s) 630
BmgT120I GGNCC 2 cut(s) 79, 127
BmiI GGNNCC 3 cut(s) 81, 128, 730
BmrFI CCNGG 1 cut(s) 630
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 3 cut(s) 292, 332, 675
BmuI ACTGGG 1 cut(s) 238
Bpu14I TTCGAA 1 cut(s) 60
BpuEI CTTGAG 2 cut(s) 479, 530
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 601, 628
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 725
Bse118I RCCGGY 2 cut(s) 662, 734
Bse1I ACTGG 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 570
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 2 cut(s) 601, 628
BseGI GGATG 2 cut(s) 439, 666
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 2 cut(s) 249, 725
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 3 cut(s) 307, 572, 676
BseYI CCCAGC 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 6 cut(s) 80, 129, 162, 171, 526, 738
BshNI GGYRCC 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 340
BsiSI CCGG 2 cut(s) 663, 735
BslI CCNNNNNNNGG 2 cut(s) 249, 725
BsmAI GTCTC 2 cut(s) 233, 556
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 6 cut(s) 80, 129, 162, 171, 526, 738
Bso31I GGTCTC 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 3 cut(s) 38, 307, 406
BspANI GGCC 6 cut(s) 80, 129, 162, 171, 526, 738
BspCNI CTCAG 1 cut(s) 179
BspFNI CGCG 1 cut(s) 612
BspLI GGNNCC 3 cut(s) 81, 128, 730
BspPI GGATC 1 cut(s) 46
BspT104I TTCGAA 1 cut(s) 60
BspT107I GGYRCC 1 cut(s) 728
BspTNI GGTCTC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 570
BsrFI RCCGGY 2 cut(s) 662, 734
BsrI ACTGG 1 cut(s) 244
BssAI RCCGGY 2 cut(s) 662, 734
BssECI CCNNGG 2 cut(s) 601, 628
BssMI GATC 3 cut(s) 38, 307, 406
BssNAI GTATAC 1 cut(s) 546
BssNI GRCGYC 1 cut(s) 729
Bst1107I GTATAC 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 4 cut(s) 67, 97, 550, 605
Bst6I CTCTTC 2 cut(s) 116, 447
BstACI GRCGYC 1 cut(s) 729
BstAPI GCANNNNNTGC 2 cut(s) 284, 569
BstBI TTCGAA 1 cut(s) 60
BstDEI CTNAG 4 cut(s) 166, 425, 446, 534
BstDSI CCRYGG 1 cut(s) 601
BstF5I GGATG 2 cut(s) 439, 666
BstFNI CGCG 1 cut(s) 612
BstH2I RGCGCY 1 cut(s) 732
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 3 cut(s) 41, 310, 409
BstMAI GTCTC 2 cut(s) 233, 556
BstMBI GATC 3 cut(s) 38, 307, 406
BstMWI GCNNNNNNNGC 7 cut(s) 168, 206, 215, 224, 284, 569, 735
BstNI CCWGG 1 cut(s) 630
BstSCI CCNGG 1 cut(s) 628
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 3 cut(s) 307, 572, 676
BstZ17I GTATAC 1 cut(s) 546
BsuRI GGCC 6 cut(s) 80, 129, 162, 171, 526, 738
BtgI CCRYGG 1 cut(s) 601
BtsCI GGATG 2 cut(s) 439, 666
BtsIMutI CAGTG 1 cut(s) 102
CfoI GCGC 1 cut(s) 731
Cfr10I RCCGGY 2 cut(s) 662, 734
Cfr13I GGNCC 2 cut(s) 79, 127
CseI GACGC 1 cut(s) 601
Csp6I GTAC 4 cut(s) 429, 470, 598, 761
CviAII CATG 1 cut(s) 540
CviQI GTAC 4 cut(s) 429, 470, 598, 761
DdeI CTNAG 4 cut(s) 166, 425, 446, 534
DinI GGCGCC 1 cut(s) 730
DpnI GATC 3 cut(s) 40, 309, 408
DpnII GATC 3 cut(s) 38, 307, 406
EaeI YGGCCR 1 cut(s) 736
Eam1104I CTCTTC 2 cut(s) 116, 447
EarI CTCTTC 2 cut(s) 116, 447
Eco31I GGTCTC 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 628
EcoT22I ATGCAT 1 cut(s) 574
EgeI GGCGCC 1 cut(s) 730
EheI GGCGCC 1 cut(s) 730
FaeI CATG 1 cut(s) 543
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FatI CATG 1 cut(s) 539
FblI GTMKAC 2 cut(s) 462, 545
Fnu4HI GCNGC 3 cut(s) 321, 561, 690
FokI GGATG 2 cut(s) 446, 653
Fsp4HI GCNGC 3 cut(s) 321, 561, 690
FspBI CTAG 4 cut(s) 111, 210, 257, 587
GlaI GCGC 1 cut(s) 730
GluI GCNGC 3 cut(s) 321, 561, 690
GsaI CCCAGC 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 732
HaeIII GGCC 6 cut(s) 80, 129, 162, 171, 526, 738
HapII CCGG 2 cut(s) 663, 735
HgaI GACGC 1 cut(s) 601
HhaI GCGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 729
Hin1II CATG 1 cut(s) 543
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HincII GTYRAC 2 cut(s) 105, 616
HindII GTYRAC 2 cut(s) 105, 616
HinfI GANTC 6 cut(s) 51, 57, 90, 155, 347, 583
HpaI GTTAAC 1 cut(s) 105
HpaII CCGG 2 cut(s) 663, 735
HphI GGTGA 3 cut(s) 176, 280, 342
Hpy166II GTNNAC 5 cut(s) 105, 271, 463, 546, 616
Hpy188I TCNGA 4 cut(s) 56, 328, 346, 757
Hpy188III TCNNGA 3 cut(s) 238, 250, 700
Hpy8I GTNNAC 5 cut(s) 105, 271, 463, 546, 616
HpyAV CCTTC 3 cut(s) 537, 664, 677
HpyCH4III ACNGT 4 cut(s) 67, 97, 550, 605
HpyCH4V TGCA 5 cut(s) 26, 278, 323, 572, 692
HpyF10VI GCNNNNNNNGC 7 cut(s) 168, 206, 215, 224, 284, 569, 735
HpyF3I CTNAG 4 cut(s) 166, 425, 446, 534
Hsp92I GRCGYC 1 cut(s) 729
Hsp92II CATG 1 cut(s) 543
HspAI GCGC 1 cut(s) 729
KasI GGCGCC 1 cut(s) 728
KspAI GTTAAC 1 cut(s) 105
Kzo9I GATC 3 cut(s) 38, 307, 406
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 3 cut(s) 307, 572, 676
LweI GCATC 3 cut(s) 292, 332, 675
MaeI CTAG 4 cut(s) 111, 210, 257, 587
MaeIII GTNAC 1 cut(s) 31
MalI GATC 3 cut(s) 40, 309, 408
MboI GATC 3 cut(s) 38, 307, 406
MboII GAAGA 4 cut(s) 133, 464, 692, 740
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 5 cut(s) 230, 357, 502, 703, 740
Mly113I GGCGCC 1 cut(s) 729
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 4 cut(s) 117, 173, 231, 503
Mph1103I ATGCAT 1 cut(s) 574
MseI TTAA 4 cut(s) 104, 363, 389, 772
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 2 cut(s) 663, 735
MspR9I CCNGG 1 cut(s) 630
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 1 cut(s) 630
MvnI CGCG 1 cut(s) 612
MwoI GCNNNNNNNGC 7 cut(s) 168, 206, 215, 224, 284, 569, 735
NarI GGCGCC 1 cut(s) 729
NdeII GATC 3 cut(s) 38, 307, 406
NlaIII CATG 1 cut(s) 543
NlaIV GGNNCC 3 cut(s) 81, 128, 730
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 574
NspV TTCGAA 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 5 cut(s) 51, 57, 90, 347, 583
PkrI GCNGC 3 cut(s) 322, 562, 691
PleI GAGTC 1 cut(s) 163
PluTI GGCGCC 1 cut(s) 732
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 628
PspFI CCCAGC 1 cut(s) 287
PspGI CCWGG 1 cut(s) 628
PspN4I GGNNCC 3 cut(s) 81, 128, 730
PspPI GGNCC 2 cut(s) 79, 127
RsaI GTAC 4 cut(s) 430, 471, 599, 762
RsaNI GTAC 4 cut(s) 429, 470, 598, 761
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 4 cut(s) 104, 363, 389, 772
SatI GCNGC 3 cut(s) 321, 561, 690
Sau3AI GATC 3 cut(s) 38, 307, 406
Sau96I GGNCC 2 cut(s) 79, 127
ScaI AGTACT 1 cut(s) 471
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 630
SduI GDGCHC 1 cut(s) 340
SetI ASST 6 cut(s) 139, 322, 447, 495, 656, 688
SfaNI GCATC 3 cut(s) 292, 332, 675
SfiI GGCCNNNNNGGCC 1 cut(s) 168
SfoI GGCGCC 1 cut(s) 730
SfuI TTCGAA 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 494, 509
SmoI CTYRAG 2 cut(s) 494, 509
Sse9I AATT 5 cut(s) 230, 357, 502, 703, 740
SspDI GGCGCC 1 cut(s) 728
SspMI CTAG 4 cut(s) 111, 210, 257, 587
StyD4I CCNGG 1 cut(s) 628
TaaI ACNGT 4 cut(s) 67, 97, 550, 605
TaqI TCGA 4 cut(s) 41, 60, 237, 409
TaqII GACCGA 1 cut(s) 741
TasI AATT 5 cut(s) 230, 357, 502, 703, 740
TatI WGTACW 2 cut(s) 469, 760
TfiI GAWTC 5 cut(s) 51, 57, 90, 347, 583
Tru1I TTAA 4 cut(s) 104, 363, 389, 772
Tru9I TTAA 4 cut(s) 104, 363, 389, 772
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 320, 560, 689
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 223, 360, 581
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 1 cut(s) 102
XapI RAATTY 2 cut(s) 502, 703
XmiI GTMKAC 2 cut(s) 462, 545
XspI CTAG 4 cut(s) 111, 210, 257, 587
ZrmI AGTACT 1 cut(s) 471
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.