FvH4_1g04550

salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000171
Physical Location & Seq
Forward (+)
2410535 .. 2412006
1472 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04550.t1

Sequence Viewer

Length: 843 bp
ATGGAGAGCAACATGAGGGTTGAGCATTTTGTGCAGTTTCTTTTGTTTCTTTGTTTAGTAGGAACCTGCGCTTCGTCCCGATCTGATCATAAGTACAGCTTTGTGCTTGTTCATGGAGCTAATCACGGTGCATGGTGTTGGTACAAGGTCTCAACTCTGCTGAAACAGTCAGGCCATAACGTTACCACTCTAGACCTTGCAGCTTCCGGCATTGACCAAACCCAGATACAGCAAGTTACTTCTGTTGCGGTTTACGTTGACCCCTTGATGAAGTTCATGGAGTCTCTCCCGCCAAAGCAGAAGGTTGTTCTGGTTGGCCACAGCATGGGTGGAGTTCCCATAGCTCTTGCCATGGAGAGGTTCCCTCACAAAATTTCTGTTGCGGTTTTCGCCACAGCTTCCATGCCTGGTCCTGATCTCACGTACGTTGCTATTCGTCAAGAGCTTGGCCGTCATTTGGATTTTGTGGACTCTCACTTCAGGTTTGATGATGGTGCTTCCAAACCTGCAACCTCGGTGCTTCGCTCTCAAGAAATCCTTGCCCCATTCTTCTACCAGCTATCGCCACCGGAGGATTTGACACTAGCGCTCACACTTGTGAGATATGCGCCGACTGTTAGAGAAGACCTGAAATTTACAAAGAAGAAATATGGATCGGTTCGTAGAGTATACATAATGTGCGATCAAGACCTTGTAATTAAGGTGGATGTTGCAAGGTTTATGATCCAAAATAATCCTCCAGATGAAGTCAAAGTGATAAATGGTTCTGATCATATGGTCATGTTCTCAAGACCACTGGAGCTCTTCTCCAACCTCCAGCAAATTGCTGAGAAGTACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

281

Amino Acids

31.5

Weight (kDa)

7.74

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 30 - 145 2.2e-11 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 33 - 265 2.2e-18 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 34 - 269 9.5e-22 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 74, 514
AccB7I CCANNNNNTGG 1 cut(s) 325
AccI GTMKAC 1 cut(s) 669
AciI CCGC 3 cut(s) 248, 290, 383
AclI AACGTT 1 cut(s) 180
AclWI GGATC 2 cut(s) 661, 718
AcoI YGGCCR 2 cut(s) 316, 448
AcsI RAATTY 2 cut(s) 372, 632
AcuI CTGAAG 1 cut(s) 463
AfaI GTAC 4 cut(s) 95, 143, 425, 836
AfeI AGCGCT 1 cut(s) 588
AfiI CCNNNNNNNGG 3 cut(s) 325, 357, 457
AjnI CCWGG 1 cut(s) 406
AleI CACNNNNGTG 1 cut(s) 596
AluBI AGCT 8 cut(s) 99, 119, 203, 344, 398, 445, 559, 802
AluI AGCT 8 cut(s) 99, 119, 203, 344, 398, 445, 559, 802
Alw21I GWGCWC 1 cut(s) 804
Alw26I GTCTC 2 cut(s) 154, 288
AlwI GGATC 2 cut(s) 661, 718
Aor51HI AGCGCT 1 cut(s) 588
AoxI GGCC 3 cut(s) 172, 316, 448
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 2 cut(s) 372, 632
AspLEI GCGC 3 cut(s) 71, 589, 610
AspS9I GGNCC 1 cut(s) 410
AvaII GGWCC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 318
BanII GRGCYC 1 cut(s) 804
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 804
BbvI GCAGC 1 cut(s) 212
BccI CCATC 1 cut(s) 485
BceAI ACGGC 1 cut(s) 435
BciT130I CCWGG 1 cut(s) 408
BclI TGATCA 2 cut(s) 85, 769
BcoDI GTCTC 2 cut(s) 154, 288
BfaI CTAG 2 cut(s) 191, 584
BfoI RGCGCY 1 cut(s) 590
BfuAI ACCTGC 2 cut(s) 74, 514
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
BmcAI AGTACT 1 cut(s) 836
Bme1390I CCNGG 1 cut(s) 408
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 2 cut(s) 64, 362
BmrFI CCNGG 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 630
BplI GAGNNNNNCTC 5 cut(s) 349, 381, 791, 823, 821
BpmI CTGGAG 3 cut(s) 723, 800, 818
BpuEI CTTGAG 2 cut(s) 513, 772
BsaAI YACGTR 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 2 cut(s) 351, 513
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 357, 457
Bse1I ACTGG 1 cut(s) 801
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 2 cut(s) 351, 513
BseGI GGATG 1 cut(s) 712
BseLI CCNNNNNNNGG 3 cut(s) 325, 357, 457
BseMII CTCAG 1 cut(s) 819
BseNI ACTGG 1 cut(s) 801
BseXI GCAGC 1 cut(s) 212
BsgI GTGCAG 1 cut(s) 53
BshFI GGCC 3 cut(s) 174, 318, 450
BsiHKAI GWGCWC 1 cut(s) 804
BsiSI CCGG 2 cut(s) 207, 569
BsiWI CGTACG 1 cut(s) 423
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 3 cut(s) 325, 357, 457
BsmAI GTCTC 2 cut(s) 154, 288
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 3 cut(s) 174, 318, 450
Bso31I GGTCTC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 804
Bsp143I GATC 7 cut(s) 80, 85, 415, 653, 682, 723, 769
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 3 cut(s) 248, 290, 383
BspANI GGCC 3 cut(s) 174, 318, 450
BspCNI CTCAG 1 cut(s) 820
BspLI GGNNCC 2 cut(s) 64, 362
BspMI ACCTGC 2 cut(s) 74, 514
BspPI GGATC 2 cut(s) 661, 718
BspQI GCTCTTC 1 cut(s) 809
BspTNI GGTCTC 1 cut(s) 154
BsrI ACTGG 1 cut(s) 801
BssECI CCNNGG 2 cut(s) 351, 513
BssMI GATC 7 cut(s) 80, 85, 415, 653, 682, 723, 769
BssNAI GTATAC 1 cut(s) 670
BssT1I CCWWGG 1 cut(s) 351
Bst1107I GTATAC 1 cut(s) 670
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 128, 168, 616
Bst6I CTCTTC 1 cut(s) 809
BstAPI GCANNNNNTGC 1 cut(s) 31
BstBAI YACGTR 1 cut(s) 423
BstDEI CTNAG 1 cut(s) 828
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 712
BstH2I RGCGCY 1 cut(s) 590
BstHHI GCGC 3 cut(s) 71, 589, 610
BstKTI GATC 7 cut(s) 83, 88, 418, 656, 685, 726, 772
BstMAI GTCTC 2 cut(s) 154, 288
BstMBI GATC 7 cut(s) 80, 85, 415, 653, 682, 723, 769
BstMWI GCNNNNNNNGC 2 cut(s) 31, 389
BstNI CCWGG 1 cut(s) 408
BstSCI CCNGG 1 cut(s) 406
BstV1I GCAGC 1 cut(s) 212
BstV2I GAAGAC 1 cut(s) 630
BstZ17I GTATAC 1 cut(s) 670
BsuRI GGCC 3 cut(s) 174, 318, 450
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 1 cut(s) 712
BtsIMutI CAGTG 1 cut(s) 794
BveI ACCTGC 2 cut(s) 74, 514
CfoI GCGC 3 cut(s) 71, 589, 610
Cfr13I GGNCC 1 cut(s) 410
Csp6I GTAC 4 cut(s) 94, 142, 424, 835
CviAII CATG 8 cut(s) 13, 113, 132, 277, 325, 352, 403, 781
CviQI GTAC 4 cut(s) 94, 142, 424, 835
DdeI CTNAG 1 cut(s) 828
DpnI GATC 7 cut(s) 82, 87, 417, 655, 684, 725, 771
DpnII GATC 7 cut(s) 80, 85, 415, 653, 682, 723, 769
EaeI YGGCCR 2 cut(s) 316, 448
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
Ecl136II GAGCTC 1 cut(s) 802
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 804
Eco31I GGTCTC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 410
Eco47III AGCGCT 1 cut(s) 588
Eco53kI GAGCTC 1 cut(s) 802
Eco57I CTGAAG 1 cut(s) 463
EcoICRI GAGCTC 1 cut(s) 802
EcoRII CCWGG 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 804
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 8 cut(s) 16, 116, 135, 280, 328, 355, 406, 784
FalI AAGNNNNNCTT 4 cut(s) 83, 115, 522, 554
FaqI GGGAC 1 cut(s) 61
FatI CATG 8 cut(s) 12, 112, 131, 276, 324, 351, 402, 780
FauI CCCGC 1 cut(s) 297
FauNDI CATATG 1 cut(s) 774
FbaI TGATCA 2 cut(s) 85, 769
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 719
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 2 cut(s) 191, 584
GlaI GCGC 3 cut(s) 70, 588, 609
GluI GCNGC 1 cut(s) 201
GsuI CTGGAG 3 cut(s) 723, 800, 818
HaeII RGCGCY 1 cut(s) 590
HaeIII GGCC 3 cut(s) 174, 318, 450
HapII CCGG 2 cut(s) 207, 569
HhaI GCGC 3 cut(s) 71, 589, 610
Hin1II CATG 8 cut(s) 16, 116, 135, 280, 328, 355, 406, 784
Hin6I GCGC 3 cut(s) 69, 587, 608
HinP1I GCGC 3 cut(s) 69, 587, 608
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 2 cut(s) 281, 470
HpaII CCGG 2 cut(s) 207, 569
Hpy166II GTNNAC 4 cut(s) 253, 259, 469, 670
Hpy188I TCNGA 2 cut(s) 85, 769
Hpy188III TCNNGA 8 cut(s) 78, 191, 413, 440, 530, 686, 740, 789
Hpy8I GTNNAC 4 cut(s) 253, 259, 469, 670
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 3 cut(s) 128, 168, 616
HpyCH4IV ACGT 4 cut(s) 180, 255, 422, 426
HpyCH4V TGCA 5 cut(s) 34, 131, 200, 509, 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 389
HpyF3I CTNAG 1 cut(s) 828
HpySE526I ACGT 4 cut(s) 180, 255, 422, 426
Hsp92II CATG 8 cut(s) 16, 116, 135, 280, 328, 355, 406, 784
HspAI GCGC 3 cut(s) 69, 587, 608
Ksp22I TGATCA 2 cut(s) 85, 769
Kzo9I GATC 7 cut(s) 80, 85, 415, 653, 682, 723, 769
LguI GCTCTTC 1 cut(s) 809
LmnI GCTCC 2 cut(s) 116, 799
Lsp1109I GCAGC 1 cut(s) 212
MaeI CTAG 2 cut(s) 191, 584
MaeII ACGT 4 cut(s) 180, 255, 422, 426
MaeIII GTNAC 2 cut(s) 181, 235
MalI GATC 7 cut(s) 82, 87, 417, 655, 684, 725, 771
MboI GATC 7 cut(s) 80, 85, 415, 653, 682, 723, 769
MboII GAAGA 4 cut(s) 541, 635, 655, 796
MhlI GDGCHC 1 cut(s) 804
MlsI TGGCCA 1 cut(s) 318
MluCI AATT 4 cut(s) 372, 632, 696, 822
MluNI TGGCCA 1 cut(s) 318
MlyI GAGTC 2 cut(s) 290, 464
MmeI TCCRAC 1 cut(s) 834
MnlI CCTC 7 cut(s) 9, 351, 375, 523, 565, 747, 824
Mox20I TGGCCA 1 cut(s) 318
MscI TGGCCA 1 cut(s) 318
MseI TTAA 1 cut(s) 699
MslI CAYNNNNRTG 1 cut(s) 596
Msp20I TGGCCA 1 cut(s) 318
MspI CCGG 2 cut(s) 207, 569
MspR9I CCNGG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 408
MwoI GCNNNNNNNGC 2 cut(s) 31, 389
NcoI CCATGG 1 cut(s) 351
NdeI CATATG 1 cut(s) 774
NdeII GATC 7 cut(s) 80, 85, 415, 653, 682, 723, 769
NlaIII CATG 8 cut(s) 16, 116, 135, 280, 328, 355, 406, 784
NlaIV GGNNCC 2 cut(s) 64, 362
OliI CACNNNNGTG 1 cut(s) 596
PciSI GCTCTTC 1 cut(s) 809
Pfl23II CGTACG 1 cut(s) 423
PflMI CCANNNNNTGG 1 cut(s) 325
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 2 cut(s) 289, 464
PpsI GAGTC 2 cut(s) 289, 464
Ppu21I YACGTR 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 804
Psp1406I AACGTT 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspLI CGTACG 1 cut(s) 423
PspN4I GGNNCC 2 cut(s) 64, 362
PspPI GGNCC 1 cut(s) 410
RsaI GTAC 4 cut(s) 95, 143, 425, 836
RsaNI GTAC 4 cut(s) 94, 142, 424, 835
RseI CAYNNNNRTG 1 cut(s) 596
SacI GAGCTC 1 cut(s) 804
SapI GCTCTTC 1 cut(s) 809
SaqAI TTAA 1 cut(s) 699
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 7 cut(s) 80, 85, 415, 653, 682, 723, 769
Sau96I GGNCC 1 cut(s) 410
ScaI AGTACT 1 cut(s) 836
SchI GAGTC 2 cut(s) 290, 464
ScrFI CCNGG 1 cut(s) 408
SduI GDGCHC 1 cut(s) 804
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 2 cut(s) 528, 787
SmoI CTYRAG 2 cut(s) 528, 787
Sse9I AATT 4 cut(s) 372, 632, 696, 822
SsiI CCGC 3 cut(s) 248, 290, 383
SspMI CTAG 2 cut(s) 191, 584
SstI GAGCTC 1 cut(s) 804
StyD4I CCNGG 1 cut(s) 406
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 3 cut(s) 128, 168, 616
TaiI ACGT 4 cut(s) 183, 258, 425, 429
TasI AATT 4 cut(s) 372, 632, 696, 822
TatI WGTACW 2 cut(s) 93, 834
Tru1I TTAA 1 cut(s) 699
Tru9I TTAA 1 cut(s) 699
TscAI CASTG 1 cut(s) 801
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 4 cut(s) 101, 265, 284, 759
TspRI CASTG 1 cut(s) 801
Van91I CCANNNNNTGG 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 410
XapI RAATTY 2 cut(s) 372, 632
XbaI TCTAGA 1 cut(s) 190
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 669
XspI CTAG 2 cut(s) 191, 584
ZrmI AGTACT 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.