FvH4_1g14930

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000171
Physical Location & Seq
Forward (+)
8370252 .. 8372309
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14930.t1

Sequence Viewer

Length: 897 bp
ATGAGACCAGATGCAGGGGAAGCAAAAATGGAGATTAAAAATATGAGGGTGAAGAAGCATTTTGTGCCATTTCTTGTATTTCTTTGCTTGGCAACAGCTAGTACCAACAACGTAGAAAGCCAGAACCTCCCATTCGGGAAGAAGCATTTTGTGCTTGTTCACGGCGCTGGTCACGGAGCATGGTGTTGGTTCAAGGTGGCAACTCTACTGGAATCCGCCGGTCACAATGTTACAGCTCTAGACATGGCGGCATCCGGAATAAACCCAACGCAGGTACAAAAAGTCCATTCGTTCTCCGATTATGCTGAGCCGTTGATCGAATTTATGGCGTCTCTACCACCACTGCATAGGGTTATCCTAGTTGGCCACAGCATGGGTGGTGCCATCATATCTCTTGCCATGGAGATGTTCCCTCTGAGAATCTCAGCTGCTGTATTCGCCACCGCTGCCATGCCTGGCCCTGATCTCACTTACTCCGCTATAGTTGCAGCGATAAACAGTGGTGCGACGTCTAGTCTTATGGATTCTCAGTTCATATATGATGACGGGCAAAACAACCCTCCAACCTCGTTGGTGTTTGGGCCTAAATTCATGTCCTCATCGTTGTACCAGCTCTCGCCTCCAGAGGACTTAACATTAGCGTTCTCATTGGTGAGACCTTCCCCACTCTTCAATGAAGAAATGAAACTCACAAAGCTGAAATACGGATTGGTTCGTAGAGTACTCATAGTAAGTGACCAAGACCGTGGTTATACCGAGCAAGTAGTAAGGTTGATGATTAGTAAAAATCCGCCAAATGAAGTGAAAGTGATCAATGGTTCAGATCACATGGTTATGGTCTCCAGACCAGTAGAGCTGTTCTCCCATCTCCAGAAGATTGCTGAGATATACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

299

Amino Acids

32.74

Weight (kDa)

6.8

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 48 - 165 2e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 49 - 279 2.9e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 50 - 286 4.7e-23 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 380
AccB7I CCANNNNNTGG 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 254
AciI CCGC 5 cut(s) 216, 248, 444, 477, 791
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 2 cut(s) 320, 587
AcyI GRCGYC 2 cut(s) 329, 509
AfaI GTAC 4 cut(s) 103, 276, 608, 723
AfiI CCNNNNNNNGG 3 cut(s) 14, 271, 373
AgsI TTSAA 2 cut(s) 193, 673
AhdI GACNNNNNGTC 1 cut(s) 513
AjnI CCWGG 1 cut(s) 454
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AloI GAACNNNNNNTCC 2 cut(s) 515, 547
AluBI AGCT 6 cut(s) 98, 236, 428, 613, 697, 856
AluI AGCT 6 cut(s) 98, 236, 428, 613, 697, 856
Alw26I GTCTC 3 cut(s) 336, 649, 844
AlwNI CAGNNNCTG 1 cut(s) 431
Aor13HI TCCGGA 1 cut(s) 254
AoxI GGCC 3 cut(s) 364, 457, 581
ApeKI GCWGC 3 cut(s) 428, 446, 488
ApoI RAATTY 2 cut(s) 320, 587
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 458, 581
AsuHPI GGTGA 2 cut(s) 61, 664
BalI TGGCCA 1 cut(s) 366
BanI GGYRCC 1 cut(s) 380
BbvI GCAGC 3 cut(s) 415, 433, 500
BccI CCATC 2 cut(s) 392, 873
BceAI ACGGC 2 cut(s) 178, 295
BciT130I CCWGG 1 cut(s) 456
BclI TGATCA 1 cut(s) 810
BcoDI GTCTC 3 cut(s) 336, 649, 844
BfaI CTAG 4 cut(s) 99, 239, 359, 513
BfmI CTRYAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 4 cut(s) 249, 429, 447, 489
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 4 cut(s) 250, 430, 448, 490
BmcAI AGTACT 1 cut(s) 723
Bme1390I CCNGG 1 cut(s) 456
BmeRI GACNNNNNGTC 1 cut(s) 513
BmgT120I GGNCC 2 cut(s) 458, 581
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 456
BmsI GCATC 1 cut(s) 260
BplI GAGNNNNNCTC 2 cut(s) 845, 877
BpmI CTGGAG 3 cut(s) 606, 826, 854
Bpu1102I GCTNAGC 1 cut(s) 306
BsaHI GRCGYC 2 cut(s) 329, 509
BsaI GGTCTC 2 cut(s) 649, 844
BsaJI CCNNGG 2 cut(s) 399, 745
BsaWI WCCGGW 1 cut(s) 254
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 271, 373
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 2 cut(s) 213, 848
BseAI TCCGGA 1 cut(s) 254
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 2 cut(s) 399, 745
BseGI GGATG 1 cut(s) 251
BseLI CCNNNNNNNGG 3 cut(s) 14, 271, 373
BseMII CTCAG 5 cut(s) 297, 407, 438, 542, 873
BseNI ACTGG 2 cut(s) 213, 848
BseXI GCAGC 3 cut(s) 415, 433, 500
BshFI GGCC 3 cut(s) 366, 459, 583
BshNI GGYRCC 1 cut(s) 380
BsiSI CCGG 2 cut(s) 219, 255
BslI CCNNNNNNNGG 3 cut(s) 14, 271, 373
BsmAI GTCTC 3 cut(s) 336, 649, 844
BsmBI CGTCTC 1 cut(s) 336
BsnI GGCC 3 cut(s) 366, 459, 583
Bso31I GGTCTC 2 cut(s) 649, 844
Bsp13I TCCGGA 1 cut(s) 254
Bsp143I GATC 4 cut(s) 315, 463, 810, 823
Bsp1720I GCTNAGC 1 cut(s) 306
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 5 cut(s) 216, 248, 444, 477, 791
BspANI GGCC 3 cut(s) 366, 459, 583
BspCNI CTCAG 5 cut(s) 298, 408, 437, 541, 874
BspEI TCCGGA 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 2 cut(s) 649, 844
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 2 cut(s) 213, 848
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 2 cut(s) 399, 745
BssMI GATC 4 cut(s) 315, 463, 810, 823
BssNI GRCGYC 2 cut(s) 329, 509
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 2 cut(s) 500, 746
Bst6I CTCTTC 1 cut(s) 674
BstACI GRCGYC 2 cut(s) 329, 509
BstAPI GCANNNNNTGC 2 cut(s) 64, 151
BstDEI CTNAG 5 cut(s) 306, 416, 424, 528, 882
BstDSI CCRYGG 2 cut(s) 399, 745
BstF5I GGATG 1 cut(s) 251
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 4 cut(s) 318, 466, 813, 826
BstMAI GTCTC 3 cut(s) 336, 649, 844
BstMBI GATC 4 cut(s) 315, 463, 810, 823
BstMWI GCNNNNNNNGC 6 cut(s) 20, 64, 151, 437, 446, 485
BstNI CCWGG 1 cut(s) 456
BstSCI CCNGG 1 cut(s) 454
BstSFI CTRYAG 1 cut(s) 480
BstV1I GCAGC 3 cut(s) 415, 433, 500
BstXI CCANNNNNNTGG 1 cut(s) 746
BsuRI GGCC 3 cut(s) 366, 459, 583
BtgI CCRYGG 2 cut(s) 399, 745
BtsCI GGATG 1 cut(s) 251
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 2 cut(s) 341, 505
BveI ACCTGC 1 cut(s) 262
CaiI CAGNNNCTG 1 cut(s) 431
CfoI GCGC 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 458, 581
CseI GACGC 1 cut(s) 318
Csp6I GTAC 4 cut(s) 102, 275, 607, 722
CviAII CATG 7 cut(s) 180, 244, 373, 400, 451, 592, 829
CviQI GTAC 4 cut(s) 102, 275, 607, 722
DdeI CTNAG 5 cut(s) 306, 416, 424, 528, 882
DpnI GATC 4 cut(s) 317, 465, 812, 825
DpnII GATC 4 cut(s) 315, 463, 810, 823
DriI GACNNNNNGTC 1 cut(s) 513
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 674
Eam1105I GACNNNNNGTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 674
EciI GGCGGA 2 cut(s) 205, 780
Eco130I CCWWGG 1 cut(s) 399
Eco31I GGTCTC 2 cut(s) 649, 844
EcoRII CCWGG 1 cut(s) 454
EcoT14I CCWWGG 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 399
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 7 cut(s) 183, 247, 376, 403, 454, 595, 832
FatI CATG 7 cut(s) 179, 243, 372, 399, 450, 591, 828
FbaI TGATCA 1 cut(s) 810
Fnu4HI GCNGC 4 cut(s) 249, 429, 447, 489
FokI GGATG 1 cut(s) 238
Fsp4HI GCNGC 4 cut(s) 249, 429, 447, 489
FspBI CTAG 4 cut(s) 99, 239, 359, 513
GlaI GCGC 1 cut(s) 166
GluI GCNGC 4 cut(s) 249, 429, 447, 489
GsuI CTGGAG 3 cut(s) 606, 826, 854
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 3 cut(s) 366, 459, 583
HapII CCGG 2 cut(s) 219, 255
HgaI GACGC 1 cut(s) 318
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 2 cut(s) 329, 509
Hin1II CATG 7 cut(s) 183, 247, 376, 403, 454, 595, 832
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HinfI GANTC 3 cut(s) 212, 420, 524
HpaII CCGG 2 cut(s) 219, 255
HphI GGTGA 2 cut(s) 61, 664
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 3 cut(s) 298, 417, 823
Hpy188III TCNNGA 6 cut(s) 136, 239, 255, 623, 843, 871
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 1 cut(s) 669
HpyCH4III ACNGT 2 cut(s) 500, 746
HpyCH4IV ACGT 2 cut(s) 111, 509
HpyCH4V TGCA 3 cut(s) 14, 346, 488
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 64, 151, 437, 446, 485
HpyF3I CTNAG 5 cut(s) 306, 416, 424, 528, 882
HpySE526I ACGT 2 cut(s) 111, 509
Hsp92I GRCGYC 2 cut(s) 329, 509
Hsp92II CATG 7 cut(s) 183, 247, 376, 403, 454, 595, 832
HspAI GCGC 1 cut(s) 165
Kpn2I TCCGGA 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 810
Kzo9I GATC 4 cut(s) 315, 463, 810, 823
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 3 cut(s) 415, 433, 500
LweI GCATC 1 cut(s) 260
MaeI CTAG 4 cut(s) 99, 239, 359, 513
MaeII ACGT 2 cut(s) 111, 509
MaeIII GTNAC 4 cut(s) 170, 221, 229, 734
MalI GATC 4 cut(s) 317, 465, 812, 825
MboI GATC 4 cut(s) 315, 463, 810, 823
MboII GAAGA 5 cut(s) 64, 151, 661, 689, 886
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 2 cut(s) 320, 587
MluNI TGGCCA 1 cut(s) 366
MmeI TCCRAC 1 cut(s) 587
MnlI CCTC 8 cut(s) 39, 137, 423, 570, 577, 607, 619, 630
Mox20I TGGCCA 1 cut(s) 366
MroI TCCGGA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 366
MseI TTAA 2 cut(s) 36, 632
MslI CAYNNNNRTG 2 cut(s) 404, 833
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 2 cut(s) 428, 446
MspI CCGG 2 cut(s) 219, 255
MspR9I CCNGG 1 cut(s) 456
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 6 cut(s) 20, 64, 151, 437, 446, 485
NcoI CCATGG 1 cut(s) 399
NdeII GATC 4 cut(s) 315, 463, 810, 823
NlaIII CATG 7 cut(s) 183, 247, 376, 403, 454, 595, 832
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 3 cut(s) 170, 221, 734
PfeI GAWTC 3 cut(s) 212, 420, 524
PflMI CCANNNNNTGG 1 cut(s) 373
PkrI GCNGC 4 cut(s) 250, 430, 448, 490
Psp6I CCWGG 1 cut(s) 454
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 2 cut(s) 458, 581
PstNI CAGNNNCTG 1 cut(s) 431
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 4 cut(s) 103, 276, 608, 723
RsaNI GTAC 4 cut(s) 102, 275, 607, 722
RseI CAYNNNNRTG 2 cut(s) 404, 833
SaqAI TTAA 2 cut(s) 36, 632
SatI GCNGC 4 cut(s) 249, 429, 447, 489
Sau3AI GATC 4 cut(s) 315, 463, 810, 823
Sau96I GGNCC 2 cut(s) 458, 581
ScaI AGTACT 1 cut(s) 723
ScrFI CCNGG 1 cut(s) 456
SfaNI GCATC 1 cut(s) 260
SfcI CTRYAG 1 cut(s) 480
SmiMI CAYNNNNRTG 2 cut(s) 404, 833
Sse9I AATT 2 cut(s) 320, 587
SsiI CCGC 5 cut(s) 216, 248, 444, 477, 791
SspMI CTAG 4 cut(s) 99, 239, 359, 513
StyD4I CCNGG 1 cut(s) 454
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 500, 746
TaiI ACGT 2 cut(s) 114, 512
TaqI TCGA 1 cut(s) 318
TasI AATT 2 cut(s) 320, 587
TatI WGTACW 1 cut(s) 721
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 3 cut(s) 212, 420, 524
Tru1I TTAA 2 cut(s) 36, 632
Tru9I TTAA 2 cut(s) 36, 632
TscAI CASTG 2 cut(s) 348, 505
TseFI GTSAC 3 cut(s) 170, 221, 734
TseI GCWGC 3 cut(s) 428, 446, 488
Tsp45I GTSAC 3 cut(s) 170, 221, 734
TspDTI ATGAA 5 cut(s) 523, 580, 690, 698, 813
TspGWI ACGGA 2 cut(s) 189, 720
TspRI CASTG 2 cut(s) 348, 505
Van91I CCANNNNNTGG 1 cut(s) 373
XapI RAATTY 2 cut(s) 320, 587
XbaI TCTAGA 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XspI CTAG 4 cut(s) 99, 239, 359, 513
ZraI GACGTC 1 cut(s) 510
ZrmI AGTACT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.