RLG00000029853

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000171
Physical Location & Seq
Reverse (-)
50201502 .. 50202643
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029853

Sequence Viewer

Length: 855 bp
ATGGAGAATATGAGAGTGAAGCATTTTGTGCCTTTTCTTTTGCTTCTTTGCTTGGCAAGAATCTGTACTAATATTGTTGAAGCTGACCAAATCTCATCTGGGAAGAAGCATTTTGTGCTGGTTCATGGAGCTGGTCACGGAGCTTGGTGTTGGTATAAGCTGGCAACTCTATTGAACTCTACAGGTCACAATGTTACAACTATAGACCTTGCAGCATCCGGTATCAACCCGATCCAGGTACAGCAAGTTCATTCGTTCTCAGACTACGTTAAGCCATTGATCAAATTTCTGGGGTCTCTGTTACCAAAAGAGAGGGTTATCCTGGTTGGCCACAGCATGGGTGGGGCCGTGATATCAATTGCCATGGAGAGGTTCCCTGAGAAAATTGCTGCAGCTGTTTATGCCACTGCTTTGATGCCTGGTCCTGCTCTGAGTTACTTGACTATTTTTGAGAAGATTCAGGAAAGATCGGTGTTGACTGACACTCAGTATAGATATGATCAGGGGGCCAACAATCCTCCAACCTCGCTACTCTTTGGACCAAAACAATTGTCTTCGAGACTGTACCAGCTATCGCCACCAGAGGATCTAGAACTAGCATTGTCATTGGTGAGATTTTCCCCTCTATTTATTGAAGAAATAAAACTCACTCAGGAGAAGTACGGATTGGTTCCAAGAGTTTTCATCGTGTGTGACCAGGACCTTTCAATAGAGGAGGATCTGCAAACGTGGATGATCAGGAAAAATCCCCCAAATGAAGTGAAGGTGATAAATGGTTCCGATCACATGGTCATGTTCTCTAGACCATTGGAGCTGTTCTCCAACCTCCTGAACATTGCTGAGAAATATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

285

Amino Acids

31.86

Weight (kDa)

6.71

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 35 - 142 2.4e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 37 - 269 3.6e-21 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 38 - 273 2.2e-23 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 337
AclWI GGATC 3 cut(s) 226, 594, 726
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 1 cut(s) 284
AfaI GTAC 4 cut(s) 67, 240, 566, 662
AfiI CCNNNNNNNGG 3 cut(s) 235, 337, 369
AgsI TTSAA 4 cut(s) 80, 175, 635, 708
AjnI CCWGG 4 cut(s) 234, 321, 418, 696
AjuI GAANNNNNNNTTGG 2 cut(s) 650, 682
AluBI AGCT 7 cut(s) 83, 131, 143, 160, 395, 571, 814
AluI AGCT 7 cut(s) 83, 131, 143, 160, 395, 571, 814
Alw26I GTCTC 2 cut(s) 300, 553
AlwI GGATC 3 cut(s) 226, 594, 726
AoxI GGCC 3 cut(s) 328, 345, 507
ApeKI GCWGC 3 cut(s) 212, 389, 392
ApoI RAATTY 1 cut(s) 284
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AspS9I GGNCC 5 cut(s) 345, 422, 507, 539, 700
AsuHPI GGTGA 2 cut(s) 622, 778
AvaII GGWCC 3 cut(s) 422, 539, 700
BalI TGGCCA 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 546
BbvI GCAGC 3 cut(s) 224, 376, 404
BceAI ACGGC 1 cut(s) 332
BciT130I CCWGG 4 cut(s) 236, 323, 420, 698
BclI TGATCA 3 cut(s) 279, 499, 735
BcoDI GTCTC 2 cut(s) 300, 553
BfaI CTAG 3 cut(s) 590, 596, 801
BfmI CTRYAG 3 cut(s) 180, 201, 390
BisI GCNGC 3 cut(s) 213, 390, 393
BlsI GCNGC 3 cut(s) 214, 391, 394
Bme1390I CCNGG 4 cut(s) 236, 323, 420, 698
Bme18I GGWCC 3 cut(s) 422, 539, 700
BmgT120I GGNCC 5 cut(s) 345, 422, 507, 539, 700
BmiI GGNNCC 5 cut(s) 346, 374, 508, 672, 778
BmrFI CCNGG 4 cut(s) 236, 323, 420, 698
BmsI GCATC 2 cut(s) 224, 405
BpiI GAAGAC 1 cut(s) 546
BplI GAGNNNNNCTC 2 cut(s) 803, 835
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 363
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 337, 369
Bse3DI GCAATG 1 cut(s) 834
BseBI CCWGG 4 cut(s) 236, 323, 420, 698
BseDI CCNNGG 1 cut(s) 363
BseGI GGATG 2 cut(s) 215, 738
BseLI CCNNNNNNNGG 3 cut(s) 235, 337, 369
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 6 cut(s) 273, 369, 422, 500, 665, 831
BseRI GAGGAG 1 cut(s) 728
BseXI GCAGC 3 cut(s) 224, 376, 404
BshFI GGCC 3 cut(s) 330, 347, 509
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 3 cut(s) 235, 337, 369
BsmAI GTCTC 2 cut(s) 300, 553
BsnI GGCC 3 cut(s) 330, 347, 509
Bso31I GGTCTC 1 cut(s) 300
Bsp143I GATC 8 cut(s) 231, 279, 467, 499, 586, 718, 735, 781
Bsp19I CCATGG 1 cut(s) 363
BspANI GGCC 3 cut(s) 330, 347, 509
BspCNI CTCAG 6 cut(s) 272, 370, 423, 499, 664, 832
BspLI GGNNCC 5 cut(s) 346, 374, 508, 672, 778
BspMAI CTGCAG 1 cut(s) 394
BspPI GGATC 3 cut(s) 226, 594, 726
BspTNI GGTCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 834
BssECI CCNNGG 1 cut(s) 363
BssMI GATC 8 cut(s) 231, 279, 467, 499, 586, 718, 735, 781
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 4 cut(s) 236, 323, 420, 698
Bst4CI ACNGT 1 cut(s) 564
BstAPI GCANNNNNTGC 2 cut(s) 28, 115
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 6 cut(s) 259, 378, 431, 486, 651, 840
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 2 cut(s) 215, 738
BstKTI GATC 8 cut(s) 234, 282, 470, 502, 589, 721, 738, 784
BstMAI GTCTC 2 cut(s) 300, 553
BstMBI GATC 8 cut(s) 231, 279, 467, 499, 586, 718, 735, 781
BstMWI GCNNNNNNNGC 3 cut(s) 28, 115, 401
BstNI CCWGG 4 cut(s) 236, 323, 420, 698
BstSCI CCNGG 4 cut(s) 234, 321, 418, 696
BstSFI CTRYAG 3 cut(s) 180, 201, 390
BstV1I GCAGC 3 cut(s) 224, 376, 404
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 2 cut(s) 586, 718
BstYI RGATCY 2 cut(s) 586, 718
BsuRI GGCC 3 cut(s) 330, 347, 509
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 2 cut(s) 215, 738
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 1 cut(s) 162
Cfr13I GGNCC 5 cut(s) 345, 422, 507, 539, 700
Csp6I GTAC 4 cut(s) 66, 239, 565, 661
CviAII CATG 5 cut(s) 125, 337, 364, 787, 793
CviQI GTAC 4 cut(s) 66, 239, 565, 661
DdeI CTNAG 6 cut(s) 259, 378, 431, 486, 651, 840
DpnI GATC 8 cut(s) 233, 281, 469, 501, 588, 720, 737, 783
DpnII GATC 8 cut(s) 231, 279, 467, 499, 586, 718, 735, 781
EaeI YGGCCR 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 363
Eco31I GGTCTC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 354
Eco47I GGWCC 3 cut(s) 422, 539, 700
EcoO109I RGGNCCY 1 cut(s) 700
EcoRII CCWGG 4 cut(s) 234, 321, 418, 696
EcoRV GATATC 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 5 cut(s) 128, 340, 367, 790, 796
FatI CATG 5 cut(s) 124, 336, 363, 786, 792
FbaI TGATCA 3 cut(s) 279, 499, 735
Fnu4HI GCNGC 3 cut(s) 213, 390, 393
FokI GGATG 2 cut(s) 202, 745
Fsp4HI GCNGC 3 cut(s) 213, 390, 393
FspBI CTAG 3 cut(s) 590, 596, 801
GluI GCNGC 3 cut(s) 213, 390, 393
HaeIII GGCC 3 cut(s) 330, 347, 509
HapII CCGG 1 cut(s) 219
Hin1II CATG 5 cut(s) 128, 340, 367, 790, 796
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HinfI GANTC 2 cut(s) 60, 457
HpaII CCGG 1 cut(s) 219
HphI GGTGA 2 cut(s) 622, 778
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 3 cut(s) 262, 432, 781
Hpy188III TCNNGA 7 cut(s) 461, 558, 590, 653, 739, 801, 829
Hpy8I GTNNAC 1 cut(s) 477
HpyAV CCTTC 1 cut(s) 757
HpyCH4III ACNGT 1 cut(s) 564
HpyCH4IV ACGT 2 cut(s) 267, 728
HpyCH4V TGCA 3 cut(s) 212, 392, 724
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 115, 401
HpyF3I CTNAG 6 cut(s) 259, 378, 431, 486, 651, 840
HpySE526I ACGT 2 cut(s) 267, 728
Hsp92II CATG 5 cut(s) 128, 340, 367, 790, 796
Ksp22I TGATCA 3 cut(s) 279, 499, 735
Kzo9I GATC 8 cut(s) 231, 279, 467, 499, 586, 718, 735, 781
LmnI GCTCC 3 cut(s) 128, 140, 811
Lsp1109I GCAGC 3 cut(s) 224, 376, 404
LweI GCATC 2 cut(s) 224, 405
MaeI CTAG 3 cut(s) 590, 596, 801
MaeII ACGT 2 cut(s) 267, 728
MaeIII GTNAC 6 cut(s) 134, 185, 193, 300, 434, 692
MalI GATC 8 cut(s) 233, 281, 469, 501, 588, 720, 737, 783
MboI GATC 8 cut(s) 231, 279, 467, 499, 586, 718, 735, 781
MboII GAAGA 4 cut(s) 115, 466, 546, 647
MfeI CAATTG 2 cut(s) 357, 548
MflI RGATCY 2 cut(s) 586, 718
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 4 cut(s) 284, 357, 384, 548
MluNI TGGCCA 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 545, 846
MnlI CCTC 9 cut(s) 306, 363, 528, 535, 577, 633, 706, 709, 836
Mox20I TGGCCA 1 cut(s) 330
MscI TGGCCA 1 cut(s) 330
MseI TTAA 2 cut(s) 270, 853
MslI CAYNNNNRTG 1 cut(s) 791
Msp20I TGGCCA 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 4 cut(s) 236, 323, 420, 698
MunI CAATTG 2 cut(s) 357, 548
MvaI CCWGG 4 cut(s) 236, 323, 420, 698
MwoI GCNNNNNNNGC 3 cut(s) 28, 115, 401
NcoI CCATGG 1 cut(s) 363
NdeII GATC 8 cut(s) 231, 279, 467, 499, 586, 718, 735, 781
NlaIII CATG 5 cut(s) 128, 340, 367, 790, 796
NlaIV GGNNCC 5 cut(s) 346, 374, 508, 672, 778
NmuCI GTSAC 3 cut(s) 134, 185, 692
PfeI GAWTC 2 cut(s) 60, 457
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 3 cut(s) 214, 391, 394
PpuMI RGGWCCY 1 cut(s) 700
Psp5II RGGWCCY 1 cut(s) 700
Psp6I CCWGG 4 cut(s) 234, 321, 418, 696
PspGI CCWGG 4 cut(s) 234, 321, 418, 696
PspN4I GGNNCC 5 cut(s) 346, 374, 508, 672, 778
PspPI GGNCC 5 cut(s) 345, 422, 507, 539, 700
PspPPI RGGWCCY 1 cut(s) 700
PsrI GAACNNNNNNTAC 2 cut(s) 231, 263
PstI CTGCAG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 586, 718
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 4 cut(s) 67, 240, 566, 662
RsaNI GTAC 4 cut(s) 66, 239, 565, 661
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 2 cut(s) 270, 853
SatI GCNGC 3 cut(s) 213, 390, 393
Sau3AI GATC 8 cut(s) 231, 279, 467, 499, 586, 718, 735, 781
Sau96I GGNCC 5 cut(s) 345, 422, 507, 539, 700
ScrFI CCNGG 4 cut(s) 236, 323, 420, 698
SfaNI GCATC 2 cut(s) 224, 405
SfcI CTRYAG 3 cut(s) 180, 201, 390
SinI GGWCC 3 cut(s) 422, 539, 700
SmiMI CAYNNNNRTG 1 cut(s) 791
Sse9I AATT 4 cut(s) 284, 357, 384, 548
SspI AATATT 2 cut(s) 73, 848
SspMI CTAG 3 cut(s) 590, 596, 801
StyD4I CCNGG 4 cut(s) 234, 321, 418, 696
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 564
TaiI ACGT 2 cut(s) 270, 731
TaqI TCGA 1 cut(s) 557
TasI AATT 4 cut(s) 284, 357, 384, 548
TatI WGTACW 1 cut(s) 65
TfiI GAWTC 2 cut(s) 60, 457
Tru1I TTAA 2 cut(s) 270, 853
Tru9I TTAA 2 cut(s) 270, 853
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 3 cut(s) 134, 185, 692
TseI GCWGC 3 cut(s) 212, 389, 392
Tsp45I GTSAC 3 cut(s) 134, 185, 692
TspDTI ATGAA 4 cut(s) 113, 239, 673, 771
TspGWI ACGGA 2 cut(s) 153, 678
TspRI CASTG 1 cut(s) 412
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 3 cut(s) 422, 539, 700
XapI RAATTY 1 cut(s) 284
XbaI TCTAGA 2 cut(s) 589, 800
XcmI CCANNNNNNNNNTGG 2 cut(s) 95, 338
XspI CTAG 3 cut(s) 590, 596, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.