MD02G1162000.v1.1

Salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000171
Physical Location & Seq
Forward (+)
13637113 .. 13637673
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1162000.v1.1.491

Sequence Viewer

Length: 561 bp
ATGATACAGAAAATATTATTGGAGAACATGACAGAGAAGCTCCTTTTGTTTTTCCTTTTATTTATTTGCTTGGCAAAAGCTAGCACCTCAACCCCATCCCCGCCCTCCAACATTCACAACCAAACTCAAAGTCCAAAACATTTTGTATTGATACATGGAGCTTGTCATGGAGCATGGAGCTGGTATAAGGTGGCAACTCTCCTCAAGGACTCAGGTCACAATGTCACAGCTCTAGACTTGGGAGCATCGGGGATCAACCCGATTCAGGTACAGCAGCTCCCTTCGTTATCGGAATTCGTCGAGCCCTTGACAAAGCTCATGGTGTCTCTACCACCAAATGAAAAGGTTATCCTTGTGGGTCACAGCTTGGGGGGCGCAGTCATATCTATTTTCATGGAGTGGTTCCCTCATAAAATTGTTGCTGCAGTATATGTCACGGCTTTTATGTATGGTCCTACTCTCAATTTCTCAACTACATATGCAGAGGTAATGAATTTCATATATACTAAAAGTGTAAACGTTCCGCTATATATCGTAAAACCTCAATACGTGAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

187

Amino Acids

20.62

Weight (kDa)

8.51

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 47 - 152 8.8e-14 alpha/beta hydrolase fold
Hydrolase_4 PF12146 47 - 154 1.2e-09 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 48 - 154 6.2e-13 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 101, 524
AclI AACGTT 1 cut(s) 519
AclWI GGATC 1 cut(s) 260
AcsI RAATTY 2 cut(s) 293, 493
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 265
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 8 cut(s) 40, 80, 161, 180, 230, 277, 316, 366
AluI AGCT 8 cut(s) 40, 80, 161, 180, 230, 277, 316, 366
Alw26I GTCTC 1 cut(s) 330
AlwI GGATC 1 cut(s) 260
ApeKI GCWGC 2 cut(s) 274, 422
ApoI RAATTY 2 cut(s) 293, 493
ArsI GACNNNNNNTTYG 2 cut(s) 115, 147
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 452
AsuNHI GCTAGC 1 cut(s) 80
AvaII GGWCC 1 cut(s) 452
BanII GRGCYC 1 cut(s) 306
BbvI GCAGC 2 cut(s) 286, 409
BccI CCATC 1 cut(s) 103
BceAI ACGGC 1 cut(s) 453
BcoDI GTCTC 1 cut(s) 330
BfaI CTAG 2 cut(s) 81, 233
BfmI CTRYAG 1 cut(s) 423
BisI GCNGC 2 cut(s) 275, 423
BlsI GCNGC 2 cut(s) 276, 424
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 404
BmsI GCATC 1 cut(s) 254
BmtI GCTAGC 1 cut(s) 84
BoxI GACNNNNGTC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 550
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 265
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 225
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 286, 409
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 330
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 1 cut(s) 252
BspACI CCGC 2 cut(s) 101, 524
BspCNI CTCAG 1 cut(s) 224
BspLI GGNNCC 1 cut(s) 404
BspMAI CTGCAG 1 cut(s) 427
BspOI GCTAGC 1 cut(s) 84
BspPI GGATC 1 cut(s) 260
BssMI GATC 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 1 cut(s) 211
BstF5I GGATG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 1 cut(s) 255
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 1 cut(s) 252
BstMWI GCNNNNNNNGC 1 cut(s) 372
BstPAI GACNNNNGTC 1 cut(s) 213
BstSFI CTRYAG 1 cut(s) 423
BstV1I GCAGC 2 cut(s) 286, 409
BtsCI GGATG 1 cut(s) 95
Cac8I GCNNGC 1 cut(s) 82
CfoI GCGC 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 452
Csp6I GTAC 1 cut(s) 269
CviAII CATG 6 cut(s) 28, 155, 167, 174, 319, 394
CviQI GTAC 1 cut(s) 269
DdeI CTNAG 1 cut(s) 211
DpnI GATC 1 cut(s) 254
DpnII GATC 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 306
FaeI CATG 6 cut(s) 31, 158, 170, 177, 322, 397
FatI CATG 6 cut(s) 27, 154, 166, 173, 318, 393
FauI CCCGC 1 cut(s) 108
FauNDI CATATG 1 cut(s) 478
Fnu4HI GCNGC 2 cut(s) 275, 423
FokI GGATG 1 cut(s) 82
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 2 cut(s) 275, 423
FspBI CTAG 2 cut(s) 81, 233
GlaI GCGC 1 cut(s) 376
GluI GCNGC 2 cut(s) 275, 423
HhaI GCGC 1 cut(s) 377
Hin1II CATG 6 cut(s) 31, 158, 170, 177, 322, 397
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HinfI GANTC 2 cut(s) 209, 262
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 291
HpyCH4IV ACGT 2 cut(s) 519, 549
HpyCH4V TGCA 2 cut(s) 425, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 372
HpyF3I CTNAG 1 cut(s) 211
HpySE526I ACGT 2 cut(s) 519, 549
Hsp92II CATG 6 cut(s) 31, 158, 170, 177, 322, 397
HspAI GCGC 1 cut(s) 375
Kzo9I GATC 1 cut(s) 252
LmnI GCTCC 6 cut(s) 45, 158, 170, 177, 242, 282
LpnPI CCDG 3 cut(s) 166, 198, 251
Lsp1109I GCAGC 2 cut(s) 286, 409
LweI GCATC 1 cut(s) 254
MaeI CTAG 2 cut(s) 81, 233
MaeII ACGT 2 cut(s) 519, 549
MaeIII GTNAC 4 cut(s) 215, 223, 359, 433
MalI GATC 1 cut(s) 254
MboI GATC 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 4 cut(s) 293, 414, 463, 493
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 6 cut(s) 97, 115, 212, 417, 478, 552
MwoI GCNNNNNNNGC 1 cut(s) 372
NdeI CATATG 1 cut(s) 478
NdeII GATC 1 cut(s) 252
NheI GCTAGC 1 cut(s) 80
NlaIII CATG 6 cut(s) 31, 158, 170, 177, 322, 397
NlaIV GGNNCC 1 cut(s) 404
NmuCI GTSAC 4 cut(s) 215, 223, 359, 433
PfeI GAWTC 1 cut(s) 262
PkrI GCNGC 2 cut(s) 276, 424
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
Ppu21I YACGTR 1 cut(s) 550
PshAI GACNNNNGTC 1 cut(s) 213
Psp1406I AACGTT 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 1 cut(s) 452
PstI CTGCAG 1 cut(s) 427
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
SatI GCNGC 2 cut(s) 275, 423
Sau3AI GATC 1 cut(s) 252
Sau96I GGNCC 1 cut(s) 452
SchI GAGTC 1 cut(s) 203
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 1 cut(s) 254
SfcI CTRYAG 1 cut(s) 423
SinI GGWCC 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 4 cut(s) 293, 414, 463, 493
SsiI CCGC 2 cut(s) 101, 524
SspI AATATT 1 cut(s) 15
SspMI CTAG 2 cut(s) 81, 233
TaiI ACGT 2 cut(s) 522, 552
TaqI TCGA 1 cut(s) 300
TasI AATT 4 cut(s) 293, 414, 463, 493
TfiI GAWTC 1 cut(s) 262
TseFI GTSAC 4 cut(s) 215, 223, 359, 433
TseI GCWGC 2 cut(s) 274, 422
Tsp45I GTSAC 4 cut(s) 215, 223, 359, 433
TspDTI ATGAA 4 cut(s) 354, 382, 487, 506
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 2 cut(s) 293, 493
XbaI TCTAGA 1 cut(s) 232
XspI CTAG 2 cut(s) 81, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.