Rmu_sc0009241.1_g000003

salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009241.1
OG0000171
Physical Location & Seq
Forward (+)
8471 .. 9626
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009241.1_g000003.1.cds

Sequence Viewer

Length: 852 bp
atgctgtttgtgttatttctttgcttattattaccaatctgtacctcatcatccccatctccaccccattcttacaaccctacccaaaacccggtcgccaagaagcatttcgtgctggttcacggagccggtcacggggcatggtgttggtacaaggtggcaactctgctgaaacagtcaggccacaacgttaccactctggaccttgcagcatcaggaatcgacccaactcagatacaacaagttcgttctttcgcggtttacattgaaccgttgacgaatttcatggagtctctcccgccaaaggagagggttgttctggttggccacagcttgggtggagtagccttatctattaccatggagaggttccctcagaaaatttctgttgcagtttttgccaccgctgccatgcctggtcctgatatcagtttcttgactatacttcaagagtttagcagtcatttggattttatggactctcaatatagatttgatgatggcgccaccaaaccagcaacctcggtactcttggggccgaaggtgctagcctcatcgttctaccagctatcgccaccagaggatttaacactagcggtcacactggtgagatttgcccctcttattacagaagaactgaaattcacaaagcatagatatggatcggttcgtaaagtatacattttgtgcgaccaagaccatgcaataaaggtggatcttgcaatgttgatgatcaacaaaaatcctccggacgaagtcaaagtgataaatggttctgatcacatggtcatgctctccagaccagtcgagcttttctccaacatcctacaaattgctgagaaatactcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

283

Amino Acids

31.3

Weight (kDa)

6.79

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 334
AccI GTMKAC 1 cut(s) 678
AccII CGCG 1 cut(s) 257
AccIII TCCGGA 1 cut(s) 748
AciI CCGC 4 cut(s) 257, 299, 405, 596
AclI AACGTT 1 cut(s) 189
AclWI GGATC 2 cut(s) 670, 723
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 3 cut(s) 280, 381, 641
AcyI GRCGYC 1 cut(s) 504
AfaI GTAC 3 cut(s) 43, 152, 528
AfiI CCNNNNNNNGG 5 cut(s) 91, 135, 304, 334, 366
AgsI TTSAA 2 cut(s) 269, 449
AjnI CCWGG 1 cut(s) 415
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AleI CACNNNNGTG 1 cut(s) 605
AluBI AGCT 3 cut(s) 333, 568, 811
AluI AGCT 3 cut(s) 333, 568, 811
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 2 cut(s) 670, 723
Aor13HI TCCGGA 1 cut(s) 748
AoxI GGCC 3 cut(s) 181, 325, 536
ApeKI GCWGC 2 cut(s) 209, 407
ApoI RAATTY 3 cut(s) 280, 381, 641
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 506
AspS9I GGNCC 3 cut(s) 202, 419, 536
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 619
AsuNHI GCTAGC 1 cut(s) 547
AvaII GGWCC 2 cut(s) 202, 419
BalI TGGCCA 1 cut(s) 327
BanI GGYRCC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 221, 394
BccI CCATC 2 cut(s) 64, 494
BciT130I CCWGG 1 cut(s) 417
BclI TGATCA 2 cut(s) 732, 778
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 2 cut(s) 548, 593
BfoI RGCGCY 1 cut(s) 507
BisI GCNGC 2 cut(s) 210, 408
BlsI GCNGC 2 cut(s) 211, 409
Bme1390I CCNGG 2 cut(s) 92, 417
Bme18I GGWCC 2 cut(s) 202, 419
BmgT120I GGNCC 3 cut(s) 202, 419, 536
BmiI GGNNCC 4 cut(s) 127, 371, 505, 537
BmrFI CCNGG 2 cut(s) 92, 417
BmsI GCATC 1 cut(s) 221
BmtI GCTAGC 1 cut(s) 551
BplI GAGNNNNNCTC 5 cut(s) 358, 390, 800, 832, 830
BpmI CTGGAG 1 cut(s) 781
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 504
BsaJI CCNNGG 2 cut(s) 360, 522
BsaWI WCCGGW 1 cut(s) 748
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 135, 304, 334, 366
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 2 cut(s) 609, 803
Bse3DI GCAATG 1 cut(s) 729
Bse8I GATNNNNATC 1 cut(s) 661
BseAI TCCGGA 1 cut(s) 748
BseBI CCWGG 1 cut(s) 417
BseDI CCNNGG 2 cut(s) 360, 522
BseGI GGATG 2 cut(s) 50, 822
BseJI GATNNNNATC 1 cut(s) 661
BseLI CCNNNNNNNGG 5 cut(s) 91, 135, 304, 334, 366
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 3 cut(s) 245, 389, 828
BseNI ACTGG 2 cut(s) 609, 803
BseXI GCAGC 2 cut(s) 221, 394
Bsh1236I CGCG 1 cut(s) 257
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 3 cut(s) 183, 327, 538
BshNI GGYRCC 1 cut(s) 503
BsiEI CGRYCG 1 cut(s) 96
BsiSI CCGG 3 cut(s) 92, 129, 749
BslI CCNNNNNNNGG 5 cut(s) 91, 135, 304, 334, 366
BsmAI GTCTC 1 cut(s) 297
BsnI GGCC 3 cut(s) 183, 327, 538
Bsp13I TCCGGA 1 cut(s) 748
Bsp143I GATC 4 cut(s) 662, 715, 732, 778
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 4 cut(s) 257, 299, 405, 596
BspANI GGCC 3 cut(s) 183, 327, 538
BspCNI CTCAG 3 cut(s) 244, 388, 829
BspEI TCCGGA 1 cut(s) 748
BspFNI CGCG 1 cut(s) 257
BspLI GGNNCC 4 cut(s) 127, 371, 505, 537
BspOI GCTAGC 1 cut(s) 551
BspPI GGATC 2 cut(s) 670, 723
BspT107I GGYRCC 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 2 cut(s) 609, 803
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 2 cut(s) 360, 522
BssMI GATC 4 cut(s) 662, 715, 732, 778
BssNAI GTATAC 1 cut(s) 679
BssNI GRCGYC 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 360
Bst1107I GTATAC 1 cut(s) 679
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 2 cut(s) 177, 273
BstACI GRCGYC 1 cut(s) 504
BstAPI GCANNNNNTGC 2 cut(s) 112, 398
BstC8I GCNNGC 1 cut(s) 549
BstDEI CTNAG 3 cut(s) 231, 375, 837
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 2 cut(s) 50, 822
BstFNI CGCG 1 cut(s) 257
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 1 cut(s) 506
BstKTI GATC 4 cut(s) 665, 718, 735, 781
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 4 cut(s) 662, 715, 732, 778
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 4 cut(s) 112, 398, 407, 544
BstNI CCWGG 1 cut(s) 417
BstSCI CCNGG 2 cut(s) 90, 415
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 2 cut(s) 221, 394
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BstZ17I GTATAC 1 cut(s) 679
BsuRI GGCC 3 cut(s) 183, 327, 538
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 2 cut(s) 50, 822
BtsIMutI CAGTG 1 cut(s) 602
Cac8I GCNNGC 1 cut(s) 549
CfoI GCGC 1 cut(s) 506
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 3 cut(s) 202, 419, 536
Csp6I GTAC 3 cut(s) 42, 151, 527
CspCI CAANNNNNGTGG 2 cut(s) 693, 728
CviAII CATG 7 cut(s) 141, 286, 361, 412, 701, 784, 790
CviJI RGCY 9 cut(s) 128, 183, 327, 333, 347, 538, 551, 568, 811
CviKI_1 RGCY 9 cut(s) 128, 183, 327, 333, 347, 538, 551, 568, 811
CviQI GTAC 3 cut(s) 42, 151, 527
DdeI CTNAG 3 cut(s) 231, 375, 837
DinI GGCGCC 1 cut(s) 505
DpnI GATC 4 cut(s) 664, 717, 734, 780
DpnII GATC 4 cut(s) 662, 715, 732, 778
EaeI YGGCCR 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 360
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 202, 419
EcoRII CCWGG 1 cut(s) 415
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 360
EgeI GGCGCC 1 cut(s) 505
EheI GGCGCC 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 7 cut(s) 144, 289, 364, 415, 704, 787, 793
FatI CATG 7 cut(s) 140, 285, 360, 411, 700, 783, 789
FauI CCCGC 1 cut(s) 306
FbaI TGATCA 2 cut(s) 732, 778
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 2 cut(s) 210, 408
FokI GGATG 2 cut(s) 37, 809
Fsp4HI GCNGC 2 cut(s) 210, 408
FspBI CTAG 2 cut(s) 548, 593
GlaI GCGC 1 cut(s) 505
GluI GCNGC 2 cut(s) 210, 408
GsuI CTGGAG 1 cut(s) 781
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 3 cut(s) 183, 327, 538
HapII CCGG 3 cut(s) 92, 129, 749
HhaI GCGC 1 cut(s) 506
Hin1I GRCGYC 1 cut(s) 504
Hin1II CATG 7 cut(s) 144, 289, 364, 415, 704, 787, 793
Hin6I GCGC 1 cut(s) 504
HinP1I GCGC 1 cut(s) 504
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 3 cut(s) 219, 290, 479
HpaII CCGG 3 cut(s) 92, 129, 749
HphI GGTGA 1 cut(s) 619
Hpy166II GTNNAC 4 cut(s) 121, 262, 276, 679
Hpy188I TCNGA 3 cut(s) 234, 378, 778
Hpy188III TCNNGA 7 cut(s) 200, 216, 422, 436, 449, 749, 798
Hpy8I GTNNAC 4 cut(s) 121, 262, 276, 679
HpyAV CCTTC 1 cut(s) 535
HpyCH4III ACNGT 2 cut(s) 177, 273
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 4 cut(s) 209, 392, 704, 722
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 398, 407, 544
HpyF3I CTNAG 3 cut(s) 231, 375, 837
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 504
Hsp92II CATG 7 cut(s) 144, 289, 364, 415, 704, 787, 793
HspAI GCGC 1 cut(s) 504
KasI GGCGCC 1 cut(s) 503
Kpn2I TCCGGA 1 cut(s) 748
Ksp22I TGATCA 2 cut(s) 732, 778
Kzo9I GATC 4 cut(s) 662, 715, 732, 778
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 2 cut(s) 221, 394
LweI GCATC 1 cut(s) 221
MaeI CTAG 2 cut(s) 548, 593
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 131, 190, 598
MalI GATC 4 cut(s) 664, 717, 734, 780
MboI GATC 4 cut(s) 662, 715, 732, 778
MboII GAAGA 1 cut(s) 644
MflI RGATCY 1 cut(s) 715
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 4 cut(s) 280, 381, 641, 831
MluNI TGGCCA 1 cut(s) 327
Mly113I GGCGCC 1 cut(s) 504
MlyI GAGTC 2 cut(s) 299, 473
MmeI TCCRAC 1 cut(s) 843
MnlI CCTC 9 cut(s) 55, 303, 360, 384, 532, 562, 574, 630, 756
Mox20I TGGCCA 1 cut(s) 327
MroI TCCGGA 1 cut(s) 748
MroXI GAANNNNTTC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 327
MseI TTAA 1 cut(s) 587
MslI CAYNNNNRTG 3 cut(s) 605, 657, 788
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 3 cut(s) 92, 129, 749
MspR9I CCNGG 2 cut(s) 92, 417
MvaI CCWGG 1 cut(s) 417
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 4 cut(s) 112, 398, 407, 544
NarI GGCGCC 1 cut(s) 504
NciI CCSGG 1 cut(s) 92
NcoI CCATGG 1 cut(s) 360
NdeII GATC 4 cut(s) 662, 715, 732, 778
NheI GCTAGC 1 cut(s) 547
NlaIII CATG 7 cut(s) 144, 289, 364, 415, 704, 787, 793
NlaIV GGNNCC 4 cut(s) 127, 371, 505, 537
NmuCI GTSAC 2 cut(s) 131, 598
OliI CACNNNNGTG 1 cut(s) 605
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 219
PflFI GACNNNGTC 1 cut(s) 755
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 2 cut(s) 211, 409
PleI GAGTC 2 cut(s) 298, 473
PluTI GGCGCC 1 cut(s) 507
PpsI GAGTC 2 cut(s) 298, 473
Psp1406I AACGTT 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 415
PspGI CCWGG 1 cut(s) 415
PspN4I GGNNCC 4 cut(s) 127, 371, 505, 537
PspPI GGNCC 3 cut(s) 202, 419, 536
PsuI RGATCY 1 cut(s) 715
PsyI GACNNNGTC 1 cut(s) 755
RsaI GTAC 3 cut(s) 43, 152, 528
RsaNI GTAC 3 cut(s) 42, 151, 527
RseI CAYNNNNRTG 3 cut(s) 605, 657, 788
SaqAI TTAA 1 cut(s) 587
SatI GCNGC 2 cut(s) 210, 408
Sau3AI GATC 4 cut(s) 662, 715, 732, 778
Sau96I GGNCC 3 cut(s) 202, 419, 536
SchI GAGTC 2 cut(s) 299, 473
ScrFI CCNGG 2 cut(s) 92, 417
SfaNI GCATC 1 cut(s) 221
SfoI GGCGCC 1 cut(s) 505
SinI GGWCC 2 cut(s) 202, 419
SmiMI CAYNNNNRTG 3 cut(s) 605, 657, 788
Sse9I AATT 4 cut(s) 280, 381, 641, 831
SsiI CCGC 4 cut(s) 257, 299, 405, 596
SspDI GGCGCC 1 cut(s) 503
SspMI CTAG 2 cut(s) 548, 593
StyD4I CCNGG 2 cut(s) 90, 415
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 2 cut(s) 177, 273
TaiI ACGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 222, 807
TasI AATT 4 cut(s) 280, 381, 641, 831
TfiI GAWTC 1 cut(s) 219
Tru1I TTAA 1 cut(s) 587
Tru9I TTAA 1 cut(s) 587
TscAI CASTG 1 cut(s) 609
TseFI GTSAC 2 cut(s) 131, 598
TseI GCWGC 2 cut(s) 209, 407
Tsp45I GTSAC 2 cut(s) 131, 598
TspDTI ATGAA 1 cut(s) 274
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 1 cut(s) 609
Tth111I GACNNNGTC 1 cut(s) 755
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 2 cut(s) 202, 419
XapI RAATTY 3 cut(s) 280, 381, 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 678
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 2 cut(s) 548, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.