Rw2G013080

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000171
Physical Location & Seq
Forward (+)
13660553 .. 13671521
10969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013080.1

Sequence Viewer

Length: 885 bp
ATGAAGGCGATAGCAAAAATGGAGATTAATAATATGAGGGTGAAGAAGCATTTTGTGCCATTTCTTGTATTTGTTTGCTTGGCAACAACCTGTAACAATATTGTAGAATGCCAAAACCACCCATTTGGGAAGAAGCATTTTGTGCTTGTTCACGGAGCTGGTCACGGAGCATGGTGTTGGTACAAGGTGACAACTCTATTGAAATCTGCCGGTCACAATGTTACAGCTCTAGACATGGCAGCATCCGGAATAAACCCGATGCAGGTGCAAGAAGTCCATTCGTTCTCGGATTATGCTGAGCCGTTGATCAAATTCATGGCTTCTCTACCACCACTACATAGGGTTATCCTAGTTGGCCACAGCATGGGTGGTGCCATCATATCTATTGCCATGGAGATGTTCCCTCAGAGGATTTCAGCTGCTGTATTCGCCACCGCTGCCATGCCTGGGCCTGCTCTAAATTACTCCACTATAGTTGCAACGATCAACGGTGGTGCGAGTACTCTTATGGACTCTCAGTTTATATATGATGATGGGCCAAACAACCCTCCGACCTCTTTACTATTTGGGTCTAACTTCATGTCCTCATCGCTGTATCAGCTCTCACCACCAGAGGATTTAACACTAGCGGTCACACTGGTGAGATTTGACCCTCTTATTACAGAAGAACTGAAATTCTCAAAGCATAGATATGGATCGGTTCGTAAAGTATACATTTTGTGCGACCAAGACCATGCAATAAAGGTGGATCTTGCAATGTTGATGATCAACAAAAATCCTCCCGATGAAGTCAAAGTGATAAATGGTTCTGATCACATGGTCATGTTCTCCAGACCACTCGAGCTTTTCTCCAACATCCTACAAATTGCTGAGAAATACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

294

Amino Acids

32.46

Weight (kDa)

7.19

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 45 - 160 9.6e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 46 - 279 1.1e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 47 - 283 5e-22 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 253
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 371
AccB7I CCANNNNNTGG 1 cut(s) 364
AccI GTMKAC 1 cut(s) 711
AccIII TCCGGA 1 cut(s) 245
AciI CCGC 2 cut(s) 435, 629
AclWI GGATC 2 cut(s) 703, 756
AcoI YGGCCR 1 cut(s) 355
AcsI RAATTY 2 cut(s) 311, 674
AfaI GTAC 2 cut(s) 182, 502
AfiI CCNNNNNNNGG 3 cut(s) 262, 364, 447
AgsI TTSAA 1 cut(s) 202
AjnI CCWGG 1 cut(s) 445
AleI CACNNNNGTG 1 cut(s) 638
AluBI AGCT 5 cut(s) 158, 227, 419, 601, 844
AluI AGCT 5 cut(s) 158, 227, 419, 601, 844
AlwI GGATC 2 cut(s) 703, 756
AlwNI CAGNNNCTG 1 cut(s) 422
Ama87I CYCGRG 1 cut(s) 839
Aor13HI TCCGGA 1 cut(s) 245
AoxI GGCC 3 cut(s) 355, 449, 536
ApeKI GCWGC 3 cut(s) 239, 419, 437
ApoI RAATTY 2 cut(s) 311, 674
AseI ATTAAT 1 cut(s) 27
AspS9I GGNCC 2 cut(s) 449, 536
AsuHPI GGTGA 4 cut(s) 52, 199, 597, 652
AvaI CYCGRG 1 cut(s) 839
BalI TGGCCA 1 cut(s) 357
BanI GGYRCC 1 cut(s) 371
BbvI GCAGC 3 cut(s) 251, 406, 424
BccI CCATC 2 cut(s) 383, 527
BceAI ACGGC 1 cut(s) 286
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 1 cut(s) 447
BclI TGATCA 3 cut(s) 306, 765, 811
BfaI CTAG 3 cut(s) 230, 350, 626
BfmI CTRYAG 1 cut(s) 471
BfuAI ACCTGC 1 cut(s) 253
BisI GCNGC 3 cut(s) 240, 420, 438
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 3 cut(s) 241, 421, 439
BmcAI AGTACT 1 cut(s) 502
Bme1390I CCNGG 1 cut(s) 447
BmeT110I CYCGRG 1 cut(s) 839
BmgT120I GGNCC 2 cut(s) 449, 536
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 1 cut(s) 447
BmsI GCATC 2 cut(s) 249, 251
BplI GAGNNNNNCTC 3 cut(s) 833, 865, 863
BpmI CTGGAG 1 cut(s) 814
Bpu1102I GCTNAGC 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 694
BsaJI CCNNGG 2 cut(s) 390, 446
BsaWI WCCGGW 1 cut(s) 245
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 364, 447
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 642
Bse3DI GCAATG 1 cut(s) 762
Bse8I GATNNNNATC 1 cut(s) 694
BseAI TCCGGA 1 cut(s) 245
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 390, 446
BseGI GGATG 2 cut(s) 242, 855
BseJI GATNNNNATC 1 cut(s) 694
BseLI CCNNNNNNNGG 3 cut(s) 262, 364, 447
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 4 cut(s) 288, 419, 530, 861
BseNI ACTGG 1 cut(s) 642
BseXI GCAGC 3 cut(s) 251, 406, 424
BshFI GGCC 3 cut(s) 357, 451, 538
BshNI GGYRCC 1 cut(s) 371
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 2 cut(s) 210, 246
BslI CCNNNNNNNGG 3 cut(s) 262, 364, 447
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 3 cut(s) 357, 451, 538
BsoBI CYCGRG 1 cut(s) 839
Bsp13I TCCGGA 1 cut(s) 245
Bsp143I GATC 6 cut(s) 306, 483, 695, 748, 765, 811
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 2 cut(s) 435, 629
BspANI GGCC 3 cut(s) 357, 451, 538
BspCNI CTCAG 4 cut(s) 289, 418, 529, 862
BspEI TCCGGA 1 cut(s) 245
BspLI GGNNCC 1 cut(s) 373
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 2 cut(s) 703, 756
BspT107I GGYRCC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 762
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 1 cut(s) 642
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 390, 446
BssMI GATC 6 cut(s) 306, 483, 695, 748, 765, 811
BssNAI GTATAC 1 cut(s) 712
BssT1I CCWWGG 1 cut(s) 390
Bst1107I GTATAC 1 cut(s) 712
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 1 cut(s) 491
BstAPI GCANNNNNTGC 2 cut(s) 55, 142
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 4 cut(s) 297, 405, 516, 870
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 2 cut(s) 242, 855
BstKTI GATC 6 cut(s) 309, 486, 698, 751, 768, 814
BstMBI GATC 6 cut(s) 306, 483, 695, 748, 765, 811
BstMWI GCNNNNNNNGC 5 cut(s) 55, 142, 428, 437, 598
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 1 cut(s) 445
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 3 cut(s) 251, 406, 424
BstX2I RGATCY 1 cut(s) 748
BstXI CCANNNNNNTGG 1 cut(s) 125
BstYI RGATCY 1 cut(s) 748
BstZ17I GTATAC 1 cut(s) 712
BsuRI GGCC 3 cut(s) 357, 451, 538
BtgI CCRYGG 1 cut(s) 390
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 2 cut(s) 242, 855
BtsIMutI CAGTG 1 cut(s) 635
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 453
CaiI CAGNNNCTG 1 cut(s) 422
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 449, 536
Csp6I GTAC 2 cut(s) 181, 501
CspCI CAANNNNNGTGG 4 cut(s) 457, 492, 726, 761
CviQI GTAC 2 cut(s) 181, 501
DdeI CTNAG 4 cut(s) 297, 405, 516, 870
DpnI GATC 6 cut(s) 308, 485, 697, 750, 767, 813
DpnII GATC 6 cut(s) 306, 483, 695, 748, 765, 811
EaeI YGGCCR 1 cut(s) 355
Eco130I CCWWGG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 839
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FbaI TGATCA 3 cut(s) 306, 765, 811
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 3 cut(s) 240, 420, 438
FokI GGATG 2 cut(s) 229, 842
Fsp4HI GCNGC 3 cut(s) 240, 420, 438
FspBI CTAG 3 cut(s) 230, 350, 626
GluI GCNGC 3 cut(s) 240, 420, 438
GsuI CTGGAG 1 cut(s) 814
HaeIII GGCC 3 cut(s) 357, 451, 538
HapII CCGG 2 cut(s) 210, 246
HinfI GANTC 1 cut(s) 512
HpaII CCGG 2 cut(s) 210, 246
HphI GGTGA 4 cut(s) 52, 199, 597, 652
Hpy166II GTNNAC 2 cut(s) 151, 712
Hpy188I TCNGA 4 cut(s) 289, 408, 552, 811
Hpy188III TCNNGA 4 cut(s) 230, 246, 782, 831
Hpy8I GTNNAC 2 cut(s) 151, 712
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4V TGCA 5 cut(s) 262, 268, 479, 737, 755
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 142, 428, 437, 598
HpyF3I CTNAG 4 cut(s) 297, 405, 516, 870
Kpn2I TCCGGA 1 cut(s) 245
Ksp22I TGATCA 3 cut(s) 306, 765, 811
Kzo9I GATC 6 cut(s) 306, 483, 695, 748, 765, 811
LmnI GCTCC 2 cut(s) 155, 167
Lsp1109I GCAGC 3 cut(s) 251, 406, 424
LweI GCATC 2 cut(s) 249, 251
MaeI CTAG 3 cut(s) 230, 350, 626
MaeIII GTNAC 6 cut(s) 92, 161, 187, 212, 220, 631
MalI GATC 6 cut(s) 308, 485, 697, 750, 767, 813
MboI GATC 6 cut(s) 306, 483, 695, 748, 765, 811
MboII GAAGA 3 cut(s) 55, 142, 677
MflI RGATCY 1 cut(s) 748
MlsI TGGCCA 1 cut(s) 357
MluCI AATT 4 cut(s) 311, 460, 674, 864
MluNI TGGCCA 1 cut(s) 357
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 2 cut(s) 575, 876
MnlI CCTC 9 cut(s) 30, 402, 414, 558, 565, 595, 607, 663, 789
Mox20I TGGCCA 1 cut(s) 357
MroI TCCGGA 1 cut(s) 245
MscI TGGCCA 1 cut(s) 357
MseI TTAA 2 cut(s) 27, 620
MslI CAYNNNNRTG 4 cut(s) 395, 638, 690, 821
Msp20I TGGCCA 1 cut(s) 357
MspA1I CMGCKG 2 cut(s) 419, 437
MspI CCGG 2 cut(s) 210, 246
MspR9I CCNGG 1 cut(s) 447
Mva1269I GAATGC 1 cut(s) 113
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 5 cut(s) 55, 142, 428, 437, 598
NcoI CCATGG 1 cut(s) 390
NdeII GATC 6 cut(s) 306, 483, 695, 748, 765, 811
NlaIV GGNNCC 1 cut(s) 373
NmuCI GTSAC 4 cut(s) 161, 187, 212, 631
OliI CACNNNNGTG 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 839
PaqCI CACCTGC 1 cut(s) 253
PctI GAATGC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 364
PkrI GCNGC 3 cut(s) 241, 421, 439
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PshBI ATTAAT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 2 cut(s) 449, 536
PspXI VCTCGAGB 1 cut(s) 839
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 748
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 2 cut(s) 182, 502
RsaNI GTAC 2 cut(s) 181, 501
RseI CAYNNNNRTG 4 cut(s) 395, 638, 690, 821
SaqAI TTAA 2 cut(s) 27, 620
SatI GCNGC 3 cut(s) 240, 420, 438
Sau3AI GATC 6 cut(s) 306, 483, 695, 748, 765, 811
Sau96I GGNCC 2 cut(s) 449, 536
ScaI AGTACT 1 cut(s) 502
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 447
SfaNI GCATC 2 cut(s) 249, 251
SfcI CTRYAG 1 cut(s) 471
Sfr274I CTCGAG 1 cut(s) 839
SlaI CTCGAG 1 cut(s) 839
SmiMI CAYNNNNRTG 4 cut(s) 395, 638, 690, 821
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 4 cut(s) 311, 460, 674, 864
SsiI CCGC 2 cut(s) 435, 629
SspI AATATT 1 cut(s) 100
SspMI CTAG 3 cut(s) 230, 350, 626
StyD4I CCNGG 1 cut(s) 445
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 1 cut(s) 491
TaqI TCGA 1 cut(s) 840
TasI AATT 4 cut(s) 311, 460, 674, 864
TatI WGTACW 1 cut(s) 500
Tru1I TTAA 2 cut(s) 27, 620
Tru9I TTAA 2 cut(s) 27, 620
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 4 cut(s) 161, 187, 212, 631
TseI GCWGC 3 cut(s) 239, 419, 437
Tsp45I GTSAC 4 cut(s) 161, 187, 212, 631
TspDTI ATGAA 4 cut(s) 17, 304, 568, 801
TspGWI ACGGA 2 cut(s) 168, 180
TspRI CASTG 1 cut(s) 642
Van91I CCANNNNNTGG 1 cut(s) 364
VspI ATTAAT 1 cut(s) 27
XapI RAATTY 2 cut(s) 311, 674
XbaI TCTAGA 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
XhoI CTCGAG 1 cut(s) 839
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 3 cut(s) 230, 350, 626
ZrmI AGTACT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.