Rh2BG174500

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000171
Physical Location & Seq
Forward (+)
15371394 .. 15384765
13372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG174500.1

Sequence Viewer

Length: 624 bp
ATGTTACAGGTGCAAGAAGTCCATTCGTTCTCGGATTATGCTGAGCCGTTGATCAAATTCATGGCTTCTCTACCACCACTACATAGGGTTATCCTAGTTGGCCACAGCATGGGTGGTGCCATCATATCTATTGCCATGGAGATGTTCCCTCAGAGGATTTCAGCTGCTGTATTCGCCACCGCTGCCATGCCTGGGCCTGCTCTAAATTACTCCACTATAGTTGCAACGATCAACAGTGGTGCGAGTACTCTTATGGACTCTCAGTTAATATATGATGATGGGCCAAACAACCCTCCGACCTCTTTTCTATTTGGGTCTAACTTCATGTCCTCATCGCTGTATCAGCTCTCACCACCAGAGGACTTAACATTAGCGTTCTCATTGGTGCGACCTTCCCCTCTCTTTAATGAAGAAATAAAACTCACAAAGCTGAAATATGGAATGGTTCGTAGAGTACTCATCGCATGTGACCAAGACCGTGGATATACCGAGCAAGTGATATTCAAAAACAATGCTCTATTTGTCTCATTTCAGCTCACAAAACAACCGATTCACCAAGCAAAGCAAAATGAAAAAATTTCACCGATAACAAACAGATCACGAACGAAACTAAATCCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

207

Amino Acids

22.94

Weight (kDa)

7.86

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 6 - 83 9.6e-07 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 10 - 163 6.6e-07 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 11 - 91 3.2e-08 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 109
AciI CCGC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 2 cut(s) 56, 576
AfaI GTAC 2 cut(s) 247, 456
AfiI CCNNNNNNNGG 2 cut(s) 109, 192
AgsI TTSAA 1 cut(s) 505
AjnI CCWGG 1 cut(s) 190
AluBI AGCT 4 cut(s) 164, 346, 430, 535
AluI AGCT 4 cut(s) 164, 346, 430, 535
Alw26I GTCTC 1 cut(s) 529
AlwNI CAGNNNCTG 1 cut(s) 167
AoxI GGCC 3 cut(s) 100, 194, 281
ApeKI GCWGC 2 cut(s) 164, 182
ApoI RAATTY 2 cut(s) 56, 576
AspS9I GGNCC 2 cut(s) 194, 281
AsuHPI GGTGA 3 cut(s) 342, 545, 573
BalI TGGCCA 1 cut(s) 102
BanI GGYRCC 1 cut(s) 116
BbvI GCAGC 2 cut(s) 151, 169
BccI CCATC 2 cut(s) 128, 272
BceAI ACGGC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 192
BclI TGATCA 1 cut(s) 51
BcoDI GTCTC 1 cut(s) 529
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 216
BisI GCNGC 2 cut(s) 165, 183
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 2 cut(s) 166, 184
BmcAI AGTACT 2 cut(s) 247, 456
Bme1390I CCNGG 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 194, 281
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 42
BsaJI CCNNGG 3 cut(s) 135, 191, 478
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 192
BseBI CCWGG 1 cut(s) 192
BseDI CCNNGG 3 cut(s) 135, 191, 478
BseLI CCNNNNNNNGG 2 cut(s) 109, 192
BseMII CTCAG 3 cut(s) 33, 164, 275
BseXI GCAGC 2 cut(s) 151, 169
BshFI GGCC 3 cut(s) 102, 196, 283
BshNI GGYRCC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 109, 192
BsmAI GTCTC 1 cut(s) 529
BsnI GGCC 3 cut(s) 102, 196, 283
Bsp143I GATC 3 cut(s) 51, 228, 596
Bsp1720I GCTNAGC 1 cut(s) 42
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 1 cut(s) 180
BspANI GGCC 3 cut(s) 102, 196, 283
BspCNI CTCAG 3 cut(s) 34, 163, 274
BspLI GGNNCC 1 cut(s) 118
BspT107I GGYRCC 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 135, 191, 478
BssMI GATC 3 cut(s) 51, 228, 596
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 236, 479
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 42, 150, 261
BstDSI CCRYGG 2 cut(s) 135, 478
BstKTI GATC 3 cut(s) 54, 231, 599
BstMAI GTCTC 1 cut(s) 529
BstMBI GATC 3 cut(s) 51, 228, 596
BstMWI GCNNNNNNNGC 3 cut(s) 173, 182, 343
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 468
BstSCI CCNGG 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 2 cut(s) 151, 169
BstXI CCANNNNNNTGG 1 cut(s) 479
BsuRI GGCC 3 cut(s) 102, 196, 283
BtgI CCRYGG 2 cut(s) 135, 478
BtgZI GCGATG 2 cut(s) 318, 445
BtsIMutI CAGTG 1 cut(s) 241
Cac8I GCNNGC 1 cut(s) 198
CaiI CAGNNNCTG 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 194, 281
Csp6I GTAC 2 cut(s) 246, 455
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviAII CATG 6 cut(s) 61, 109, 136, 187, 325, 465
CviJI RGCY 9 cut(s) 46, 65, 102, 164, 196, 283, 346, 430, 535
CviKI_1 RGCY 9 cut(s) 46, 65, 102, 164, 196, 283, 346, 430, 535
CviQI GTAC 2 cut(s) 246, 455
DdeI CTNAG 3 cut(s) 42, 150, 261
DpnI GATC 3 cut(s) 53, 230, 598
DpnII GATC 3 cut(s) 51, 228, 596
EaeI YGGCCR 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 6 cut(s) 64, 112, 139, 190, 328, 468
FatI CATG 6 cut(s) 60, 108, 135, 186, 324, 464
FbaI TGATCA 1 cut(s) 51
Fnu4HI GCNGC 2 cut(s) 165, 183
Fsp4HI GCNGC 2 cut(s) 165, 183
FspBI CTAG 1 cut(s) 95
GluI GCNGC 2 cut(s) 165, 183
HaeIII GGCC 3 cut(s) 102, 196, 283
Hin1II CATG 6 cut(s) 64, 112, 139, 190, 328, 468
HinfI GANTC 2 cut(s) 257, 550
HphI GGTGA 3 cut(s) 342, 545, 573
Hpy188I TCNGA 3 cut(s) 34, 153, 297
Hpy188III TCNNGA 2 cut(s) 600, 617
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 2 cut(s) 236, 479
HpyCH4V TGCA 2 cut(s) 13, 224
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 182, 343
HpyF3I CTNAG 3 cut(s) 42, 150, 261
Hsp92II CATG 6 cut(s) 64, 112, 139, 190, 328, 468
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 3 cut(s) 51, 228, 596
LpnPI CCDG 4 cut(s) 177, 204, 210, 369
Lsp1109I GCAGC 2 cut(s) 151, 169
MaeI CTAG 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 3, 467
MalI GATC 3 cut(s) 53, 230, 598
MboI GATC 3 cut(s) 51, 228, 596
MboII GAAGA 1 cut(s) 422
MlsI TGGCCA 1 cut(s) 102
MluCI AATT 3 cut(s) 56, 205, 576
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 7 cut(s) 147, 159, 303, 310, 340, 352, 408
Mox20I TGGCCA 1 cut(s) 102
MscI TGGCCA 1 cut(s) 102
MseI TTAA 3 cut(s) 266, 365, 405
MslI CAYNNNNRTG 1 cut(s) 140
Msp20I TGGCCA 1 cut(s) 102
MspA1I CMGCKG 2 cut(s) 164, 182
MspR9I CCNGG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 3 cut(s) 173, 182, 343
NcoI CCATGG 1 cut(s) 135
NdeII GATC 3 cut(s) 51, 228, 596
NlaIII CATG 6 cut(s) 64, 112, 139, 190, 328, 468
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 467
NspI RCATGY 1 cut(s) 468
PfeI GAWTC 1 cut(s) 550
PflMI CCANNNNNTGG 1 cut(s) 109
PkrI GCNGC 2 cut(s) 166, 184
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 2 cut(s) 194, 281
PstNI CAGNNNCTG 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 2 cut(s) 247, 456
RsaNI GTAC 2 cut(s) 246, 455
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 3 cut(s) 266, 365, 405
SatI GCNGC 2 cut(s) 165, 183
Sau3AI GATC 3 cut(s) 51, 228, 596
Sau96I GGNCC 2 cut(s) 194, 281
ScaI AGTACT 2 cut(s) 247, 456
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 192
SetI ASST 7 cut(s) 12, 166, 302, 348, 394, 432, 537
SfcI CTRYAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 140
Sse9I AATT 3 cut(s) 56, 205, 576
SsiI CCGC 1 cut(s) 180
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 190
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 2 cut(s) 236, 479
TasI AATT 3 cut(s) 56, 205, 576
TatI WGTACW 2 cut(s) 245, 454
TfiI GAWTC 1 cut(s) 550
Tru1I TTAA 3 cut(s) 266, 365, 405
Tru9I TTAA 3 cut(s) 266, 365, 405
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 2 cut(s) 164, 182
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 4 cut(s) 49, 313, 423, 585
TspRI CASTG 1 cut(s) 241
Van91I CCANNNNNTGG 1 cut(s) 109
XapI RAATTY 2 cut(s) 56, 576
XceI RCATGY 1 cut(s) 468
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XspI CTAG 1 cut(s) 95
ZrmI AGTACT 2 cut(s) 247, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.