Rorug02G0118200

Salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000171
Physical Location & Seq
Forward (+)
10108123 .. 10110928
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0118200.1

Sequence Viewer

Length: 2409 bp
ATGAACGACACTCTCTCTCCTCCGCCGTCCCCCGGCGATGGCGACTACGCCGCCTGGTACGGCAACATCCAGTACCTCCTCAACATCTCCGCCATCGGCTCCTTCTTCTGCCTCTTCATCTTCCTCTTCGTCAAGCTCCGCTCCGACCACCGCCGCATGCCGGGACCCTCCGCCCTCGCCGCCAAGCTCCTCGCCGTCTGGCACGCCACTGGCCGCGAAATCGCCCGCCACTGCGGCGCCGACGCCGCCCAATTTCTCCTAATCGAAGGCGGCAGCTGCGGCCTGCTGCTCTCCCTCGCCGTCCTCGCCGTGCTGGTCATGCTTCCGTTGAATCTCTACGCCGGGACGGCCGTGCTCGGCGATCAATTCTCGCAGACGACGATAAACCATATCGAGAAAGGTTCAGCTTTACTTTGGGTGCATTTTGTTTTTCTGGTTGTGGTTGTTGTTTTCGTGCATTTTGGTATTTCTGCTATGGAGTCAAGGTTGAAGATCACTAGGATTAGGGATGGGAATGGGAATATGAGTGACCCAGGATCGGATTCCACAGCTCTTTTTACTATAATGGTGCAGGGGATACCGAAAACTATAGGGAGTGATGGGACTGTGTTGCATGAGTATTTTCAGCATAAGTATCCTGGTAAGGTTTATAAGGTTGTTTTGCCAATGGATTTGTGTGCATTGGAAGAATTAGCGTCGGAGTTGGTTAAGGTTAGGCATGAAATTGCTTGGTTGGTTGCGAAAATTGACTCCCGGCTCTTGCCTGAAGAGAGTATAGAAAATGGATATGGACCGGCTTTGTCAGAGGGAGCATGGGGCTGGGCTTTTAATCTATGGAGGAAGGTGAAGGATTTAGGGCATCATGCTGTGGCTAGCTTGGGTTACACGGATGATAGGAGATTAGGAGAGTTGCAGGAATTGAGAGCTGAATTGGAGACTGAACTGGCTGCTTATAAAGAAGGCCGTGCAGTGGGTGCTGGAGTTGCGTTTGTGGTGTTTAAGGATGTGTACACTGCTAATAAAGCGGTTCAGGACTTTCGACATGAGAAGAAGAGGCGGATAGGGAAGTTCTTTTCTCTCATGGAGTTGCGTTTACAGAGAAATCACTGGAAAGTTGAGCAGGCACCATTGGCAACAGACATTTATTGGAATCATTTGGGATCGTCCAAGGTCTCACTGAAGTTGCGGAGAGTTCTTGTGAATACATGCTTACTGTTGATTCTTCTATTTTTTAGCTCTCCTCTTGCAATTATCAGTGCTGTGAAAAGTGCTGGACGGATCATAAATGCAGAAGCTATGGACAATGCCGATTTGTGGTTAGCTTGGTTTCAGAGCTCAAGCTGGCTAGGGAGCCTCATCTTTCAGTTTATGCCCAATGTCATTATTTTTATAAGCATGTATATCGTAATTCCATCAGCGCTTTCCTATCTCTCCAAGTTTGAACGACATCTTACAGTTTCAGGAGAGCAGAGAGCCGCACTTCTTAAGATGGTTTGTTTCTTCCTGGTAAATCTTATTCTTCTTAAGGGTCTGGTTGAATCATCTTTAGAGAGTGCCCTGCTAAAAATGGGAAGGTGCTATTTGGATGGAGAAGATTGCAAGAGGATTGAGCAGTACATGAGTGCATCATTTTTGTCAAGATCCTGCCTTTCCTCTCTGGCATTTTTGATTACAAGTACGTTTTTGGGGATATCATTTGATCTATTGGCTCCGATCCCTTGGATAAAAAGGAAGATTCAGAAGTTCCGGAAGAATGACATGCTGCAGCTGGTCCCAGAACAAAGTGAAGAGTATCCATTAGAAACTCAGGAGCCTGATACTCTACAAAGACCCTTGATTGTGGAGAACACATATTATGATTCTCCTAGGCTGAATGGAATGGATATGCCGGGACATGATCTTTCTGAATACCCCATCAACAGAACCTCTACAGCCCCCAAGCAGACATTCGATTTTGCGCAATATTATGCATTCAATTTGACAATATTTGCCCTCACATTTATCTATTCTTCATTTGCTCCACTTGTGGTTCCGGTTGGTGCAATATATTTCGGGTACAGGTACGTGGTTGATAAGTACAACTTTCTGTTTGTCTATCGGGTCCGCGGCTTTCCTGCTGGAAATGATGGGAAGTTGATGGATACAGTCTTGAGCATCATGCGTTTCTGTGTTGATTTGTACCTGCTTGCAATGCTCTTGTTCTTTTCGGTTCATGGAGACTCAACAAAGCTGCAAGCAATATTCACACTTGGGGTGTTAGTAATGTACAAACTGTCGCCTTCTCACAATGATAGATTTCATCCTGCTGTTTTGGAAGGCATACAAACTGTAGACAGTTTTGTGGATGGACCCATTGATTATGAGGTCTTTTCACAGCCTAAGTTTGATTGGGATACGTATTACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

802

Amino Acids

90.32

Weight (kDa)

6.69

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 49 - 155 8.3e-12 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 186 - 385 5.3e-13 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 396 - 581 1.2e-11 Calcium-dependent channel, 7TM region, putative phosphate
RSN1_7TM PF02714 647 - 734 4.7e-12 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 651, 954, 1391
Acc16I TGCGCA 1 cut(s) 1959
Acc36I ACCTGC 1 cut(s) 2190
AccB1I GGYRCC 2 cut(s) 236, 1123
AccBSI CCGCTC 1 cut(s) 141
AccI GTMKAC 1 cut(s) 2331
AccII CGCG 2 cut(s) 216, 2106
AccIII TCCGGA 1 cut(s) 1746
AclWI GGATC 5 cut(s) 544, 1168, 1286, 1635, 1708
AcoI YGGCCR 2 cut(s) 211, 348
AcuI CTGAAG 2 cut(s) 786, 1199
AcyI GRCGYC 2 cut(s) 237, 243
AfeI AGCGCT 1 cut(s) 1419
AfiI CCNNNNNNNGG 6 cut(s) 32, 38, 160, 538, 970, 1314
AflII CTTAAG 2 cut(s) 1484, 1523
AgsI TTSAA 5 cut(s) 331, 490, 1442, 1538, 1975
AjnI CCWGG 4 cut(s) 53, 532, 637, 1503
AjuI GAANNNNNNNTTGG 2 cut(s) 1931, 1963
Alw21I GWGCWC 2 cut(s) 357, 1337
Alw26I GTCTC 3 cut(s) 929, 1177, 2211
AlwI GGATC 5 cut(s) 544, 1168, 1286, 1635, 1708
Aor13HI TCCGGA 1 cut(s) 1746
Aor51HI AGCGCT 1 cut(s) 1419
AoxI GGCC 4 cut(s) 211, 280, 348, 961
ApeKI GCWGC 7 cut(s) 273, 276, 286, 947, 1762, 1765, 2230
AspA2I CCTAGG 1 cut(s) 1865
AspLEI GCGC 3 cut(s) 239, 1420, 1960
AspS9I GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
AsuC2I CCSGG 5 cut(s) 33, 162, 343, 754, 1890
AsuHPI GGTGA 1 cut(s) 856
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
AvrII CCTAGG 1 cut(s) 1865
BaeGI GKGCMC 1 cut(s) 1558
BanI GGYRCC 2 cut(s) 236, 1123
BanII GRGCYC 1 cut(s) 1337
Bbv12I GWGCWC 2 cut(s) 357, 1337
BbvI GCAGC 7 cut(s) 263, 273, 285, 934, 1749, 1777, 2217
BceAI ACGGC 8 cut(s) 10, 76, 179, 284, 293, 335, 363, 948
BcgI CGANNNNNNTGC 2 cut(s) 1384, 1418
BciT130I CCWGG 4 cut(s) 55, 534, 639, 1505
BciVI GTATCC 5 cut(s) 570, 645, 1803, 2134, 2386
BcnI CCSGG 5 cut(s) 33, 162, 343, 754, 1890
BcoDI GTCTC 3 cut(s) 929, 1177, 2211
BfaI CTAG 4 cut(s) 498, 873, 1346, 1866
BfmI CTRYAG 4 cut(s) 588, 1763, 1929, 2328
BfoI RGCGCY 2 cut(s) 240, 1421
BfrI CTTAAG 2 cut(s) 1484, 1523
BfuAI ACCTGC 1 cut(s) 2190
BfuI GTATCC 5 cut(s) 570, 645, 1803, 2134, 2386
BglI GCCNNNNNGGC 2 cut(s) 234, 347
BlnI CCTAGG 1 cut(s) 1865
Bme1390I CCNGG 9 cut(s) 33, 55, 162, 343, 534, 639, 754, 1505, 1890
Bme18I GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
BmgT120I GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
BmrFI CCNGG 9 cut(s) 33, 55, 162, 343, 534, 639, 754, 1505, 1890
BmsI GCATC 3 cut(s) 868, 1634, 2163
BmtI GCTAGC 1 cut(s) 876
BpmI CTGGAG 1 cut(s) 999
BpuEI CTTGAG 2 cut(s) 1321, 2170
BpuMI CCSGG 5 cut(s) 33, 162, 343, 754, 1890
BsaAI YACGTR 2 cut(s) 2065, 2397
BsaHI GRCGYC 2 cut(s) 237, 243
BsaI GGTCTC 1 cut(s) 1177
BsaJI CCNNGG 6 cut(s) 31, 532, 1167, 1718, 1865, 2104
BsaWI WCCGGW 2 cut(s) 1746, 2032
BsaXI ACNNNNNCTCC 3 cut(s) 31, 470, 500
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 38, 160, 538, 970, 1314
Bse118I RCCGGY 1 cut(s) 793
Bse1I ACTGG 4 cut(s) 70, 214, 948, 1112
Bse3DI GCAATG 1 cut(s) 2196
BseAI TCCGGA 1 cut(s) 1746
BseBI CCWGG 4 cut(s) 55, 534, 639, 1505
BseDI CCNNGG 6 cut(s) 31, 532, 1167, 1718, 1865, 2104
BseGI GGATG 7 cut(s) 66, 514, 895, 1009, 1591, 2299, 2350
BseLI CCNNNNNNNGG 6 cut(s) 32, 38, 160, 538, 970, 1314
BseMI GCAATG 1 cut(s) 2196
BseMII CTCAG 1 cut(s) 1820
BseNI ACTGG 4 cut(s) 70, 214, 948, 1112
BseRI GAGGAG 4 cut(s) 9, 68, 179, 1230
BseSI GKGCMC 1 cut(s) 1558
BseX3I CGGCCG 1 cut(s) 348
BseXI GCAGC 7 cut(s) 263, 273, 285, 934, 1749, 1777, 2217
BseYI CCCAGC 1 cut(s) 819
BsgI GTGCAG 2 cut(s) 590, 987
Bsh1236I CGCG 2 cut(s) 216, 2106
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 4 cut(s) 213, 282, 350, 963
BshNI GGYRCC 2 cut(s) 236, 1123
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 2 cut(s) 357, 1337
BsiSI CCGG 8 cut(s) 33, 161, 342, 754, 794, 1747, 1889, 2033
BslFI GGGAC 6 cut(s) 13, 177, 358, 616, 1757, 1905
BslI CCNNNNNNNGG 6 cut(s) 32, 38, 160, 538, 970, 1314
BsmAI GTCTC 3 cut(s) 929, 1177, 2211
BsmFI GGGAC 6 cut(s) 13, 177, 358, 616, 1757, 1905
BsmI GAATGC 1 cut(s) 1970
BsnI GGCC 4 cut(s) 213, 282, 350, 963
Bso31I GGTCTC 1 cut(s) 1177
Bsp1286I GDGCHC 3 cut(s) 357, 1337, 1558
Bsp13I TCCGGA 1 cut(s) 1746
Bsp1407I TGTACA 2 cut(s) 1008, 2265
Bsp143I GATC 9 cut(s) 361, 492, 536, 1160, 1278, 1640, 1699, 1713, 1897
BspANI GGCC 4 cut(s) 213, 282, 350, 963
BspCNI CTCAG 1 cut(s) 1819
BspEI TCCGGA 1 cut(s) 1746
BspFNI CGCG 2 cut(s) 216, 2106
BspMAI CTGCAG 1 cut(s) 1767
BspMI ACCTGC 1 cut(s) 2190
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 5 cut(s) 544, 1168, 1286, 1635, 1708
BspT107I GGYRCC 2 cut(s) 236, 1123
BspTI CTTAAG 2 cut(s) 1484, 1523
BspTNI GGTCTC 1 cut(s) 1177
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 2196
BsrFI RCCGGY 1 cut(s) 793
BsrGI TGTACA 2 cut(s) 1008, 2265
BsrI ACTGG 4 cut(s) 70, 214, 948, 1112
BssAI RCCGGY 1 cut(s) 793
BssECI CCNNGG 6 cut(s) 31, 532, 1167, 1718, 1865, 2104
BssMI GATC 9 cut(s) 361, 492, 536, 1160, 1278, 1640, 1699, 1713, 1897
BssNI GRCGYC 2 cut(s) 237, 243
BssT1I CCWWGG 3 cut(s) 1167, 1718, 1865
Bst2UI CCWGG 4 cut(s) 55, 534, 639, 1505
Bst4CI ACNGT 7 cut(s) 607, 1215, 1456, 2146, 2274, 2329, 2336
Bst6I CTCTTC 5 cut(s) 119, 131, 762, 1046, 1782
BstACI GRCGYC 2 cut(s) 237, 243
BstAFI CTTAAG 2 cut(s) 1484, 1523
BstAPI GCANNNNNTGC 1 cut(s) 974
BstAUI TGTACA 2 cut(s) 1008, 2265
BstBAI YACGTR 2 cut(s) 2065, 2397
BstC8I GCNNGC 9 cut(s) 158, 204, 226, 284, 874, 1122, 1343, 2187, 2235
BstDEI CTNAG 2 cut(s) 1806, 2379
BstDSI CCRYGG 1 cut(s) 2104
BstF5I GGATG 7 cut(s) 66, 514, 895, 1009, 1591, 2299, 2350
BstFNI CGCG 2 cut(s) 216, 2106
BstH2I RGCGCY 2 cut(s) 240, 1421
BstHHI GCGC 3 cut(s) 239, 1420, 1960
BstKTI GATC 9 cut(s) 364, 495, 539, 1163, 1281, 1643, 1702, 1716, 1900
BstMAI GTCTC 3 cut(s) 929, 1177, 2211
BstMBI GATC 9 cut(s) 361, 492, 536, 1160, 1278, 1640, 1699, 1713, 1897
BstMCI CGRYCG 1 cut(s) 351
BstNI CCWGG 4 cut(s) 55, 534, 639, 1505
BstNSI RCATGY 4 cut(s) 160, 1209, 1399, 1762
BstSCI CCNGG 9 cut(s) 31, 53, 160, 341, 532, 637, 752, 1503, 1888
BstSFI CTRYAG 4 cut(s) 588, 1763, 1929, 2328
BstSLI GKGCMC 1 cut(s) 1558
BstSNI TACGTA 1 cut(s) 2397
BstUI CGCG 2 cut(s) 216, 2106
BstV1I GCAGC 7 cut(s) 263, 273, 285, 934, 1749, 1777, 2217
BstX2I RGATCY 1 cut(s) 1640
BstYI RGATCY 1 cut(s) 1640
BstZI CGGCCG 1 cut(s) 348
BsuI GTATCC 5 cut(s) 570, 645, 1803, 2134, 2386
BsuRI GGCC 4 cut(s) 213, 282, 350, 963
BtgI CCRYGG 1 cut(s) 2104
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 7 cut(s) 66, 514, 895, 1009, 1591, 2299, 2350
BtsI GCAGTG 3 cut(s) 229, 975, 1011
BtsIMutI CAGTG 7 cut(s) 207, 229, 975, 1011, 1105, 1175, 1261
BveI ACCTGC 1 cut(s) 2190
Cac8I GCNNGC 9 cut(s) 158, 204, 226, 284, 874, 1122, 1343, 2187, 2235
CfoI GCGC 3 cut(s) 239, 1420, 1960
Cfr10I RCCGGY 1 cut(s) 793
Cfr13I GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
Cfr42I CCGCGG 1 cut(s) 2107
CseI GACGC 2 cut(s) 251, 684
DdeI CTNAG 2 cut(s) 1806, 2379
DinI GGCGCC 1 cut(s) 238
DpnI GATC 9 cut(s) 363, 494, 538, 1162, 1280, 1642, 1701, 1715, 1899
DpnII GATC 9 cut(s) 361, 492, 536, 1160, 1278, 1640, 1699, 1713, 1897
EaeI YGGCCR 2 cut(s) 211, 348
EagI CGGCCG 1 cut(s) 348
Eam1104I CTCTTC 5 cut(s) 119, 131, 762, 1046, 1782
EarI CTCTTC 5 cut(s) 119, 131, 762, 1046, 1782
EciI GGCGGA 4 cut(s) 12, 79, 160, 1072
Ecl136II GAGCTC 1 cut(s) 1335
EclXI CGGCCG 1 cut(s) 348
Eco105I TACGTA 1 cut(s) 2397
Eco130I CCWWGG 3 cut(s) 1167, 1718, 1865
Eco24I GRGCYC 1 cut(s) 1337
Eco31I GGTCTC 1 cut(s) 1177
Eco32I GATATC 1 cut(s) 1692
Eco47I GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
Eco47III AGCGCT 1 cut(s) 1419
Eco52I CGGCCG 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 1335
Eco57I CTGAAG 2 cut(s) 786, 1199
EcoICRI GAGCTC 1 cut(s) 1335
EcoO109I RGGNCCY 1 cut(s) 164
EcoRII CCWGG 4 cut(s) 53, 532, 637, 1503
EcoRV GATATC 1 cut(s) 1692
EcoT14I CCWWGG 3 cut(s) 1167, 1718, 1865
EcoT22I ATGCAT 1 cut(s) 1972
EcoT38I GRGCYC 1 cut(s) 1337
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
ErhI CCWWGG 3 cut(s) 1167, 1718, 1865
FalI AAGNNNNNCTT 2 cut(s) 2066, 2098
FaqI GGGAC 6 cut(s) 13, 177, 358, 616, 1757, 1905
FauI CCCGC 1 cut(s) 233
FblI GTMKAC 1 cut(s) 2331
FokI GGATG 7 cut(s) 53, 521, 902, 1016, 1598, 2286, 2357
FriOI GRGCYC 1 cut(s) 1337
FspBI CTAG 4 cut(s) 498, 873, 1346, 1866
FspI TGCGCA 1 cut(s) 1959
GlaI GCGC 3 cut(s) 238, 1419, 1959
GsaI CCCAGC 1 cut(s) 823
GsuI CTGGAG 1 cut(s) 999
HaeII RGCGCY 2 cut(s) 240, 1421
HaeIII GGCC 4 cut(s) 213, 282, 350, 963
HapII CCGG 8 cut(s) 33, 161, 342, 754, 794, 1747, 1889, 2033
HgaI GACGC 2 cut(s) 251, 684
HhaI GCGC 3 cut(s) 239, 1420, 1960
Hin1I GRCGYC 2 cut(s) 237, 243
Hin6I GCGC 3 cut(s) 237, 1418, 1958
HinP1I GCGC 3 cut(s) 237, 1418, 1958
HpaII CCGG 8 cut(s) 33, 161, 342, 754, 794, 1747, 1889, 2033
HphI GGTGA 1 cut(s) 856
Hpy166II GTNNAC 4 cut(s) 1009, 1011, 1094, 2332
Hpy188I TCNGA 8 cut(s) 145, 541, 700, 805, 1332, 1713, 1740, 1906
Hpy188III TCNNGA 7 cut(s) 394, 1031, 1461, 1638, 1747, 1808, 2149
Hpy8I GTNNAC 4 cut(s) 1009, 1011, 1094, 2332
Hpy99I CGWCG 3 cut(s) 245, 382, 700
HpyAV CCTTC 8 cut(s) 112, 260, 835, 841, 953, 1566, 2289, 2309
HpyCH4III ACNGT 7 cut(s) 607, 1215, 1456, 2146, 2274, 2329, 2336
HpyCH4IV ACGT 3 cut(s) 1679, 2064, 2396
HpyF3I CTNAG 2 cut(s) 1806, 2379
HpySE526I ACGT 3 cut(s) 1679, 2064, 2396
Hsp92I GRCGYC 2 cut(s) 237, 243
HspAI GCGC 3 cut(s) 237, 1418, 1958
KasI GGCGCC 1 cut(s) 236
KflI GGGWCCC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 1746
KspI CCGCGG 1 cut(s) 2107
Kzo9I GATC 9 cut(s) 361, 492, 536, 1160, 1278, 1640, 1699, 1713, 1897
LmnI GCTCC 9 cut(s) 104, 141, 146, 192, 809, 1350, 1714, 1810, 2023
Lsp1109I GCAGC 7 cut(s) 263, 273, 285, 934, 1749, 1777, 2217
LweI GCATC 3 cut(s) 868, 1634, 2163
MaeI CTAG 4 cut(s) 498, 873, 1346, 1866
MaeII ACGT 3 cut(s) 1679, 2064, 2396
MaeIII GTNAC 2 cut(s) 527, 881
MalI GATC 9 cut(s) 363, 494, 538, 1162, 1280, 1642, 1701, 1715, 1899
MbiI CCGCTC 1 cut(s) 141
MboI GATC 9 cut(s) 361, 492, 536, 1160, 1278, 1640, 1699, 1713, 1897
MflI RGATCY 1 cut(s) 1640
MhlI GDGCHC 3 cut(s) 357, 1337, 1558
Mly113I GGCGCC 1 cut(s) 237
MlyI GAGTC 3 cut(s) 488, 743, 2213
MmeI TCCRAC 2 cut(s) 168, 678
Mph1103I ATGCAT 1 cut(s) 1972
MroI TCCGGA 1 cut(s) 1746
MseI TTAA 5 cut(s) 708, 828, 999, 1485, 1524
MspA1I CMGCKG 3 cut(s) 276, 1768, 2106
MspCI CTTAAG 2 cut(s) 1484, 1523
MspI CCGG 8 cut(s) 33, 161, 342, 754, 794, 1747, 1889, 2033
MspR9I CCNGG 9 cut(s) 33, 55, 162, 343, 534, 639, 754, 1505, 1890
Mva1269I GAATGC 1 cut(s) 1970
MvaI CCWGG 4 cut(s) 55, 534, 639, 1505
MvnI CGCG 2 cut(s) 216, 2106
NarI GGCGCC 1 cut(s) 237
NciI CCSGG 5 cut(s) 33, 162, 343, 754, 1890
NdeII GATC 9 cut(s) 361, 492, 536, 1160, 1278, 1640, 1699, 1713, 1897
NheI GCTAGC 1 cut(s) 872
NmeAIII GCCGAG 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 527
NsbI TGCGCA 1 cut(s) 1959
NsiI ATGCAT 1 cut(s) 1972
NspI RCATGY 4 cut(s) 160, 1209, 1399, 1762
PaeI GCATGC 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 377
PctI GAATGC 1 cut(s) 1970
PfeI GAWTC 7 cut(s) 331, 542, 1150, 1219, 1538, 1735, 1859
PleI GAGTC 3 cut(s) 487, 743, 2213
PluTI GGCGCC 1 cut(s) 240
PpsI GAGTC 3 cut(s) 487, 743, 2213
Ppu21I YACGTR 2 cut(s) 2065, 2397
PpuMI RGGWCCY 1 cut(s) 164
PsiI TTATAA 3 cut(s) 651, 954, 1391
Psp124BI GAGCTC 1 cut(s) 1337
Psp5II RGGWCCY 1 cut(s) 164
Psp6I CCWGG 4 cut(s) 53, 532, 637, 1503
PspFI CCCAGC 1 cut(s) 819
PspGI CCWGG 4 cut(s) 53, 532, 637, 1503
PspPI GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
PspPPI RGGWCCY 1 cut(s) 164
PstI CTGCAG 1 cut(s) 1767
PsuI RGATCY 1 cut(s) 1640
PvuII CAGCTG 2 cut(s) 276, 1768
SacI GAGCTC 1 cut(s) 1337
SacII CCGCGG 1 cut(s) 2107
SaqAI TTAA 5 cut(s) 708, 828, 999, 1485, 1524
Sau3AI GATC 9 cut(s) 361, 492, 536, 1160, 1278, 1640, 1699, 1713, 1897
Sau96I GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
SchI GAGTC 3 cut(s) 488, 743, 2213
ScrFI CCNGG 9 cut(s) 33, 55, 162, 343, 534, 639, 754, 1505, 1890
SduI GDGCHC 3 cut(s) 357, 1337, 1558
SfaNI GCATC 3 cut(s) 868, 1634, 2163
SfcI CTRYAG 4 cut(s) 588, 1763, 1929, 2328
SfoI GGCGCC 1 cut(s) 238
Sfr303I CCGCGG 1 cut(s) 2107
SgrBI CCGCGG 1 cut(s) 2107
SinI GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
SmlI CTYRAG 4 cut(s) 1336, 1484, 1523, 2149
SmoI CTYRAG 4 cut(s) 1336, 1484, 1523, 2149
SnaBI TACGTA 1 cut(s) 2397
SphI GCATGC 1 cut(s) 160
SspDI GGCGCC 1 cut(s) 236
SspI AATATT 3 cut(s) 1964, 1986, 2241
SspMI CTAG 4 cut(s) 498, 873, 1346, 1866
SstI GAGCTC 1 cut(s) 1337
StyD4I CCNGG 9 cut(s) 31, 53, 160, 341, 532, 637, 752, 1503, 1888
StyI CCWWGG 3 cut(s) 1167, 1718, 1865
TaaI ACNGT 7 cut(s) 607, 1215, 1456, 2146, 2274, 2329, 2336
TaiI ACGT 3 cut(s) 1682, 2067, 2399
TaqI TCGA 4 cut(s) 264, 393, 1039, 1950
TatI WGTACW 4 cut(s) 1008, 1614, 2076, 2265
TfiI GAWTC 7 cut(s) 331, 542, 1150, 1219, 1538, 1735, 1859
Tru1I TTAA 5 cut(s) 708, 828, 999, 1485, 1524
Tru9I TTAA 5 cut(s) 708, 828, 999, 1485, 1524
TscAI CASTG 7 cut(s) 214, 236, 975, 1018, 1112, 1182, 1261
TseFI GTSAC 1 cut(s) 527
TseI GCWGC 7 cut(s) 273, 276, 286, 947, 1762, 1765, 2230
Tsp45I GTSAC 1 cut(s) 527
TspDTI ATGAA 6 cut(s) 17, 106, 735, 2001, 2201, 2288
TspGWI ACGGA 3 cut(s) 315, 902, 1291
TspRI CASTG 7 cut(s) 214, 236, 975, 1018, 1112, 1182, 1261
Vha464I CTTAAG 2 cut(s) 1484, 1523
VpaK11BI GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
XceI RCATGY 4 cut(s) 160, 1209, 1399, 1762
XmaJI CCTAGG 1 cut(s) 1865
XmiI GTMKAC 1 cut(s) 2331
XspI CTAG 4 cut(s) 498, 873, 1346, 1866
Zsp2I ATGCAT 1 cut(s) 1972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.